Publications by authors named "Fang Xu"

842 Publications

Deep learning-driven scaffold hopping in the discovery of Akt kinase inhibitors.

Chem Commun (Camb) 2021 Sep 24. Epub 2021 Sep 24.

College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.

Scaffold hopping has been widely used in drug discovery and is a topic of high interest. Here a deep conditional transformer neural network, SyntaLinker, was applied for the scaffold hopping of a phase III clinical Akt inhibitor, AZD5363. A number of novel scaffolds were generated and compound 1a as a proof-of-concept was synthesized and validated by biochemical assay. Further structure-based optimization of 1a led to a novel Akt inhibitor with high potency (Akt1 IC = 88 nM) and antitumor activities.
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http://dx.doi.org/10.1039/d1cc03392aDOI Listing
September 2021

Activation of TIR signalling boosts pattern-triggered immunity.

Nature 2021 Sep 20. Epub 2021 Sep 20.

Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.

Plant immune responses are mainly activated by two types of receptor. Plasma membrane-localized pattern recognition receptors (PRRs) perceive extracellular microbial features, and nucleotide-binding leucine-rich repeat receptors (NLRs) recognize intracellular effector proteins from pathogens. NLRs possessing N-terminal Toll/interleukin-1 receptor (TIR) domains activate defence responses via the NADase activity of the TIR domain. Here we report that activation of TIR signalling plays a key role in PRR-mediated pattern-triggered immunity (PTI). TIR signalling mutants exhibit attenuated PTI responses and decreased resistance against pathogens. Consistently, PTI is compromised in plants with reduced NLR levels. Treatment with PTI elicitor flg22 or nlp20 rapidly induces many genes encoding TIR domain-containing proteins, which is likely to be responsible for activating TIR signalling during PTI. Overall, our study reveals that TIR signalling activation is an important mechanism for boosting plant defence during PTI.
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http://dx.doi.org/10.1038/s41586-021-03987-1DOI Listing
September 2021

Discovery of potential biomarkers for human atherosclerotic abdominal aortic aneurysm through untargeted metabolomics and transcriptomics.

J Zhejiang Univ Sci B 2021 Sept 15;22(9):733-745

Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Abdominal aortic aneurysm (AAA) and atherosclerosis (AS) have considerable similarities in clinical risk factors and molecular pathogenesis. The aim of our study was to investigate the differences between AAA and AS from the perspective of metabolomics, and to explore the potential mechanisms of differential metabolites via integration analysis with transcriptomics. Plasma samples from 32 AAA and 32 AS patients were applied to characterize the metabolite profiles using untargeted liquid chromatography-mass spectrometry (LC-MS). A total of 18 remarkably different metabolites were identified, and a combination of seven metabolites could potentially serve as a biomarker to distinguish AAA and AS, with an area under the curve (AUC) of 0.93. Subsequently, we analyzed both the metabolomics and transcriptomics data and found that seven metabolites, especially 2'-deoxy-D-ribose (2dDR), were significantly correlated with differentially expressed genes. In conclusion, our study presents a comprehensive landscape of plasma metabolites in AAA and AS patients, and provides a research direction for pathogenetic mechanisms in atherosclerotic AAA.
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http://dx.doi.org/10.1631/jzus.B2000713DOI Listing
September 2021

Materials in Advanced Design of Personal Protective Equipment (PPE): A Review.

Mater Today Adv 2021 Sep 8:100171. Epub 2021 Sep 8.

Research Center for Smart Wearable Technology, Institute of Textiles and Clothing, The Hong Kong Polytechnic University, 999077, Hong Kong, China.

The outbreak of Covid-19 pandemic has aroused tremendous attention towards personal protective equipment (PPE) in both scientific research and industrial manufacture. Despite decades of development in PPE design and fabrication, there's still much room for further optimization, in terms of both protection performance and wear comfort. Interdisciplinary efforts have been devoted in this research field in recent years. Significantly, the innovation of materials, which brings about improved performance and versatile new functions for PPEs, has been widely adopted in PPE design. In this minireview, recent progress in the development of novel materials and structural designs for PPE application are presented in detail, with the introduction of various material-based strategies for different PPE type, as well as the examples which applies auxiliary components into face masks to enrich the functionalities and improve the personal feelings in pandemic period.
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http://dx.doi.org/10.1016/j.mtadv.2021.100171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423993PMC
September 2021

Molybdenum oxide-iron, cobalt, copper alloy hybrid as efficient bifunctional catalyst for alkali water electrolysis.

J Colloid Interface Sci 2021 Aug 28;606(Pt 2):1662-1672. Epub 2021 Aug 28.

School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Henan Xinxiang 453007, PR China. Electronic address:

Efficient and durable non-precious catalyst for both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is pivotal for practical water electrolysis toward clean hydrogen fuel. Herein, a molybdenum oxide-FeCoCu alloy hybrid (MoO-FeCoCu) catalyst was designed by polyoxometallate (POM) molecular cluster mediated solvothermal alcoholysis and ammonolysis of metal salts followed by pyrolytic reduction treatment. The HER efficiency is substantially enhanced by the ternary alloy component, which is more close to the benchmark Pt/C catalyst, and the HER catalytic stability is also superior to Pt/C catalyst. Moreover, the MoO-FeCoCu demonstrates high catalytic efficiency and rather good durability for OER. Benefitted by the bifunctional catalytic behaviors for HER and OER, the symmetric water electrolyzer based on the MoO-FeCoCu electrode requires a low driving voltage of 1.69 V to deliver a response current density of 10 mA cm, which is comparable to that based on the benchmark Pt/C HER cathode and RuO OER anode. The current work offers a feasible way to design efficient bifunctional catalyst for water electrolysis via POM mediated co-assembly and calcination treatment.
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http://dx.doi.org/10.1016/j.jcis.2021.08.174DOI Listing
August 2021

Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues.

Biol Chem 2021 Sep 9. Epub 2021 Sep 9.

School of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou510632, China.

Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols and , and can be further developed to support a thiol-quantification assay.
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http://dx.doi.org/10.1515/hsz-2021-0189DOI Listing
September 2021

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway.

Front Pharmacol 2021 23;12:693982. Epub 2021 Aug 23.

Department of Pharmacy, The First People's Hospital of Yongkang Affiliated to Hangzhou Medical College, Jinhua, China.

Ulcerative colitis (UC) is a chronic inflammatory disease that affects the colon, and its incidence is on the rise worldwide. Resveratrol (RSV), a polyphenolic compound, was recently indicated to exert anti-inflammatory effects on UC. Consequently, the current study was conducted to investigate the mechanism of RSV on alleviating UC in mice by mediating intestinal microflora homeostasis. First, potential targets that RSV may regulate UC were screened using the TCMSP database. Next, mice were treated differently, specifically subjected to sham-operation and dextran sulfate sodium (DSS) induction, and then treated or untreated with RSV. Disease Activity Index (DAI) and Hematoxylin-Eosin (HE) staining were employed to analyze the pathological changes of mice colon. In addition, the expression patterns of inflammatory factors in spleen tissues were detected using ELISA, while the protein expression patterns of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and vascular endothelial growth factor A (VEGFA) in colon tissues were determined by means of immunohistochemistry (IHC) and Western blot analysis. Moreover, changes in intestinal flora and metabolite diversity in UC were analyzed by metabonomics. It was found that RSV played inhibitory roles in the PI3K/Akt pathway in mice. Meanwhile, the administration of RSV induced downregulated the expressions of TNF-α, IFN-γ, IL-1β, IL-6, and IL-4. The six floras of and were significantly enriched in UC, while , , , and were found to be enriched in control samples. Lastly, it was noted that could regulate the intestinal flora structure of UC mice through triacylglycerol biosynthesis, glycerol phosphate shuttle, cardiolipin biosynthesis, and other metabolic pathways to improve UC in mice. Altogether, our findings indicate that RSV suppressed the activation of the PI3K/Akt pathway and reduced the VEGFA gene expression to alleviate UC in mice.
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http://dx.doi.org/10.3389/fphar.2021.693982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419259PMC
August 2021

Driving mechanism of drift-step-recovery diodes.

Rev Sci Instrum 2021 Aug;92(8):084702

Science and Technology on Plasma Laboratory, Air Force Engineering University, Xi'an 710038, People's Republic of China.

Parameters governing forward and reverse pumping of a device mechanism have an important influence on the cutoff and output characteristics of a drift-step-recovery diode (DSRD). Therefore, we analyzed the driving circuit for the DSRD that was used to adjust pumping parameter values. With multiple modules in parallel, a driving method exploiting narrow-pulse pumping is proposed in developing a high-repetition-frequency DSRD pulse generator. In experiments and simulations, one key factor affecting the working stability of this pulse generator operating at repetition frequencies in the megahertz range was found that helped in further optimizing the parameter settings of our 2 kV DSRD driving circuit. From this insight, a 2 kV-5 MHz all-solid-state high-repetition-frequency pulse generator based on the DSRD was developed.
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http://dx.doi.org/10.1063/5.0048768DOI Listing
August 2021

Urinary Proteomics Identifying Novel Biomarkers for the Diagnosis and Phenotyping of Carotid Artery Stenosis.

Front Mol Biosci 2021 10;8:714706. Epub 2021 Aug 10.

State Key Laboratory of Complex Severe and Rare Disease, Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Carotid artery stenosis (CAS) is caused by the formation of atherosclerotic plaques inside the arterial wall and accounts for 20-30% of all strokes. The development of an early, noninvasive diagnostic method and the identification of high-risk patients for ischemic stroke is essential to the management of CAS in clinical practice. We used the data-independent acquisition (DIA) technique to conduct a urinary proteomic study in patients with CAS and healthy controls. We identified the potential diagnosis and risk stratification biomarkers of CAS. And Ingenuity pathway analysis was used for functional annotation of differentially expressed proteins (DEPs). Furthermore, receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic values of DEPs. A total of 194 DEPs were identified between CAS patients and healthy controls by DIA quantification. The bioinformatics analysis showed that these DEPs were correlated with the pathogenesis of CAS. We further identified 32 DEPs in symptomatic CAS compared to asymptomatic CAS, and biological function analysis revealed that these proteins are mainly related to immune/inflammatory pathways. Finally, a biomarker panel of six proteins (ACP2, PLD3, HLA-C, GGH, CALML3, and IL2RB) exhibited potential diagnostic value in CAS and good discriminative power for differentiating symptomatic and asymptomatic CAS with high sensitivity and specificity. Our study identified novel potential urinary biomarkers for noninvasive early screening and risk stratification of CAS.
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http://dx.doi.org/10.3389/fmolb.2021.714706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383446PMC
August 2021

Global Evolution of Skeletal Muscle Tissue Engineering: A Scientometric Research.

Tissue Eng Part C Methods 2021 Sep;27(9):497-511

School of Physical Education, China University of Geosciences, Wuhan, P.R. China.

Skeletal muscle tissue engineering (SMTE) is of great significance in the study of skeletal muscle physiology and pathology, which could be used in skeletal muscle graft. The scientometric analysis of SMTE can help researchers to quickly understand the evolutive history, status, novelties, and trend of this field. In this study, we performed a scientometric study that can be used to construct and visualize networks of SMTE using VOSviewer. A total of 1384 documents published between 1994 and 2020 were retrieved and analyzed. Our results showed that number of publications in SMTE has increased slowly from 1994 to 2014 and has increased rapidly from 2015 to 2020. The geographical distribution of publications in terms of total publications about SMTE is concentrated in Europe and the United States. The most productive institution was University of Michigan, while Harvard University and the University of Pittsburgh were ranked the second and third places. SMTE influenced a wide spectrum of disciplines, including and . In addition, the research hotspot of SMTE was expanding from seed cells to the combination with advanced strategies (electrostatic spinning, bioprinting, and materials) for emulating the highly bionic engineered skeletal muscle tissues. This study provided a unique perspective for understanding the history and trends of SMTE, which could help to promote the rapid development of the field. Impact statement Skeletal muscle tissue engineering (SMTE), which acts as an important branch of tissue engineering, hold a great promise in the study of skeletal muscle physiology and pathology. The field of SMTE has developed rapidly in recent decades while still lacking studies based on scientometric methods. This article provided the first scientometric study of SMTE from development trends and evolution of the field. The results indicated that the field of SMTE was experiencing rapid growth and had a significant impact on multiple fields, particularly in and .
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http://dx.doi.org/10.1089/ten.TEC.2021.0156DOI Listing
September 2021

Two-year and multi-site field trials to evaluate soil amendments for controlling cadmium accumulation in rice grain.

Environ Pollut 2021 Aug 8;289:117918. Epub 2021 Aug 8.

Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing, 210095, China.

Representing the staple crop for half of the world population, rice can accumulate high levels of cadmium (Cd) in its grain, posing concerns on food safety. Different soil amendments have been proposed to decrease Cd accumulation in rice grain by either decreasing soil Cd availability, introducing competitive ions on Cd uptake, or down-regulating the expression of transporters for Cd uptake. However, the effectiveness of soil amendments applied alone or in combinations needs to be tested under field conditions. Here, we present results of field trials with two rice cultivars differing in Cd accumulation grown at three field sites in southern China in two years, to investigate the effects of two Mn-containing soil amendments (MnO, Mn-loaded biochar (MB)), Si fertilizer (Si), limestone, and KSO, as well as interactions among MnO, Si, and limestone on decreasing Cd accumulation in rice grain. We found that single applications of MnO or MB to acidic soils low in Mn decreased grain Cd concentrations by 44-53 % or 78-82 %, respectively, over two years without decrease in performance. These effects were comparable to or greater than those induced by limestone liming alone (45-62 %). Strong interactions between MnO and limestone resulting from their influence on soil extractable Cd and Mn led to non-additive effects on lowering grain Cd. MB addition minimized grain Cd concentrations, primarily by increasing extractable and dissolved Mn concentrations, but also by decreasing Cd extractability in soil. In comparison, Si and KSO amendments affected grain Cd levels only weakly. We conclude that the amendments that decrease labile Cd and increase labile Mn in soils are most effective at reducing Cd accumulation in rice grain, thus contributing to food safety.
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http://dx.doi.org/10.1016/j.envpol.2021.117918DOI Listing
August 2021

Computed tomography nomogram to predict a high-risk intraductal papillary mucinous neoplasm of the pancreas.

Abdom Radiol (NY) 2021 Aug 18. Epub 2021 Aug 18.

Department of Radiology, Changhai Hospital, Naval Medical University, Changhai Road 168, Shanghai, 200434, China.

Objective: The intraductal papillary mucinous neoplasm (IPMN) of the pancreas is regarded as a precursor to pancreatic cancer; this study aimed to develop and validate a model based on CT characteristics for the non-invasive prediction of the high-risk IPMN of the pancreas.

Materials And Methods: In this retrospective study, all patients underwent multidetector CT and surgical resection. A prediction model was developed based on a training set consisting of 136 patients with low-risk IPMN and 85 patients with high-risk IPMN between October 2012 and April 2019, and a multivariable logistic regression model was adopted to establish a nomogram. The nomogram performance was determined by its discrimination, calibration, and clinical usefulness. The model was validated in 80 consecutive patients between May 2019 and April 2020, of which 47 and 33 patients had low-risk and high-risk IPMNs, respectively.

Results: The multivariable logistic regression model of CT characteristics included the enhancing mural nodule size, the main pancreatic duct (MPD) diameter, the abrupt change in caliber of the MPD with distal pancreatic atrophy, cyst size, thickened enhancing cyst wall, and the presence of lymphadenopathy. The individualized prediction nomogram using these predictors of the high-risk IPMN achieved an area under the curve (AUC) of 0.92 (95% CI 0.88-0.95) in the training set and 0.87 (95% CI 0.79-0.95) in the validation set. The decision curve analysis demonstrated that the nomogram was clinically useful.

Conclusion: The CT nomogram, which is a non-invasive predictive tool, can preoperatively predict the risk of malignant IPMN and help identify patients who require a surgical procedure.
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http://dx.doi.org/10.1007/s00261-021-03247-wDOI Listing
August 2021

A Porphyrin-Based Covalent Organic Framework for Metal-Free Photocatalytic Aerobic Oxidative Coupling of Amines.

Chemistry 2021 Aug 12. Epub 2021 Aug 12.

Institute of Molecular Sciences and Engineering, Institute of Frontier and Interdisciplinary Science, Shandong University, Qingdao, 266237, P. R. China.

Imines are important intermediates in drug synthesis. Photocatalytic aerobic oxidative coupling of amines has been considered as a clean and promising way to produce imines and has attracted great attention. Herein, we designed and synthesized a novel two-dimensional porphyrin-based covalent organic framework (Por-BC-COF) which adopts an AA stacking mode with excellent crystallinity, high Brunauer-Emmett-Teller surface areas (1200 m  g ), wide light absorption range (200-1300 nm) and good stability in a variety of organic solvents. Por-BC-COF can be used as a metal-free heterogeneous photocatalyst for the photocatalytic oxidation of amines to imines under visible light and red light with a high yield (97 %). This work presents a novel and efficient COF photocatalyst in the application of light-driven organic synthesis.
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http://dx.doi.org/10.1002/chem.202102239DOI Listing
August 2021

Preoperative Radiomics Approach to Evaluating Tumor-Infiltrating CD8 T Cells in Patients With Pancreatic Ductal Adenocarcinoma Using Noncontrast Magnetic Resonance Imaging.

J Magn Reson Imaging 2021 Aug 6. Epub 2021 Aug 6.

Department of Radiology, Changhai Hospital, Navy Medical University, Shanghai, China.

Background: CD8 T cell in pancreatic ductal adenocarcinoma (PDAC) is closely related to the prognosis and treatment response of patients. Accurate preoperative CD8 T-cell expression can better identify the population benefitting from immunotherapy.

Purpose: To develop and validate a machine learning classifier based on noncontrast magnetic resonance imaging (MRI) for the preoperative prediction of CD8 T-cell expression in patients with PDAC.

Study Type: Retrospective cohort study.

Population: Overall, 114 patients with PDAC undergoing MR scan and surgical resection; 97 and 47 patients in the training and validation cohorts. FIELD STRENGTH/SEQUENCE/3 T: Breath-hold single-shot fast-spin echo T2-weighted sequence and noncontrast T1-weighted fat-suppressed sequences.

Assessment: CD8 T-cell expression was quantified using immunohistochemistry. For each patient, 2232 radiomics features were extracted from noncontrast T1- and T2-weighted images and reduced using the Wilcoxon rank-sum test and least absolute shrinkage and selection operator method. Linear discriminative analysis was used to construct radiomics and mixed models. Model performance was determined by its discriminative ability, calibration, and clinical utility.

Statistical Tests: Kaplan-Meier estimates, Student's t-test, the Kruskal-Wallis H test, and the chi-square test, receiver operating characteristic curve, and decision curve analysis.

Results: A log-rank test showed that the survival duration in the CD8-high group (25.51 months) was significantly longer than that in the CD8-low group (22.92 months). The mixed model included all MRI characteristics and 13 selected radiomics features, and the area under the curve (AUC) was 0.89 (95% confidence interval [CI], 0.77-0.92) and 0.69 (95% CI, 0.53-0.82) in the training and validation cohorts. The radiomics model included 13 radiomics features, which showed good discrimination in the training cohort (AUC, 0.85; 95% CI, 0.77-0.92) and the validation cohort (AUC, 0.76; 95% CI, 0.61-0.87).

Data Conclusions: This study developed a noncontrast MRI-based radiomics model that can preoperatively determine CD8 T-cell expression in patients with PDAC and potentially immunotherapy planning.

Evidence Level: 5 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.27871DOI Listing
August 2021

Identification of proteins associated with development of psoriatic arthritis in peripheral blood mononuclear cells: a quantitative iTRAQ-based proteomics study.

J Transl Med 2021 08 3;19(1):331. Epub 2021 Aug 3.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Biomarkers for distinguishing psoriatic arthritis (PsA) from psoriasis without arthritis (PsO) are still lacking.

Methods: We applied isobaric tags for relative and absolute quantification (iTRAQ) and LC-MS/MS to analyze the proteome profile of peripheral blood mononuclear cells (PBMCs) collected from patients with PsO, patients with PsA, and healthy controls. Bioinformatics analysis and western blotting were performed to identify and validate differentially expressed proteins.

Results: We identified 389, 199, 291, and 60 significantly differentially expressed proteins (adj.p < 0.05) in the comparison of all psoriatic patients versus healthy controls, PsO group versus healthy controls, PsA group versus healthy controls, and PsA group versus PsO group, respectively. Among these proteins, 14 proteins may represent promising biomarkers for PsA: SIRT2, NAA50, ARF6, ADPRHL2, SF3B6, SH3KBP1, UBA3, SCP2, RPS5, NUDT5, NCBP1, SYNE1, NDUFB7, HTATSF1. Furthermore, western blotting confirmed that SIRT2 expression was significantly higher in PBMCs from PsA patients than PsO and healthy controls, and was negatively correlated with the phosphorylation of p38 mitogen-activated protein kinase (p-p38MAPK; p = 0.006, r = - 0.582).

Conclusions: This pilot study provided a broad characterization of the proteome of PBMCs in PsA as compared to PsO and healthy controls, which may help to provide prospective strategies for PsA diagnosis.
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http://dx.doi.org/10.1186/s12967-021-03006-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336315PMC
August 2021

Marine-Derived Xyloketal Compound Ameliorates MPP-Induced Neuronal Injury through Regulating of the IRE1/XBP1 Signaling Pathway.

ACS Chem Neurosci 2021 08 2;12(16):3101-3111. Epub 2021 Aug 2.

School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

The IRE1/XBP1 signaling pathway is the most conserved component of the endoplasmic reticulum unfolded protein response (UPR). Activating this branch to correct defects in ER proteostasis is regarded as a promising anti-Parkinson's disease (PD) strategy. is a marine-derived xyloketal B analog which exhibited potential neuroprotective activities in previous research studies; however, the molecular mechanism underneath its protective effect remains unknown. Herein, a transcriptomic approach was introduced to explore the protective mechanism of . RNA microarray profiling was conducted based on an MPP-induced PD model, and bioinformatics analyses including GO enrichment and PPI network analysis were subsequently performed. In particular, the recovery of the impaired UPR was highlighted as a main physiological change caused by , and a cluster of genes including and family genes which are involved in the IRE1/XBP1 branch of the UPR were identified as the key genes related to its neuroprotective effect. The transcription levels of these key genes were validated by RT-qPCR assays. Further results showed that enhanced the phosphorylation of IRE1, the splicing of xbp-1 mRNA, and the translation of XBP1S and boosted the expression level of the downstream targets of the IRE1/XBP1 signaling pathway. Moreover, it was also demonstrated that accelerated the scavenging of misfolded α-synuclein and attenuated the correlative mitochondrial dysfunction. Finally, the protective effect of against MPP-induced dopaminergic neuronal loss was assessed. Taken together, these results revealed that plays its neuroprotective role through regulating of the IRE1/XBP1 signaling pathway and laid the foundation for its further development as an ER proteostasis-regulating agent.
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http://dx.doi.org/10.1021/acschemneuro.1c00362DOI Listing
August 2021

Single-Center Experience in the Endovascular Management of the Combination of Isolated Common and Internal Iliac Artery Aneurysms.

Front Surg 2021 15;8:693233. Epub 2021 Jul 15.

Department of Vascular Surgery, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The combination of isolated common and internal iliac artery aneurysms (CIIAA) are rare, life-threatening, abnormal conditions with relatively complex treatment. This study aimed to evaluate the clinical characteristics and treatment outcomes of CIIAA. We retrospectively reviewed 26 patients with CIIAA consecutively treated between January 2010 and August 2020 at Peking Union Medical College Hospital. Demographic, clinical characteristic, treatment strategy and outcome data were collected and analyzed. Twenty-six patients (24 men and 2 women) with a mean age of 70 years were included. There was a total of 72 aneurysms, and the mean diameters of the common iliac artery aneurysms (CIAA) and internal iliac artery aneurysms (IIAA) were 36 and 38 mm, respectively. Ten patients (38%) presented with bilateral CIAA and unilateral IIAA, and eight (31%) had CIAA with ipsilateral IIAA. All patients were treated with endovascular repair, and the overall primary technical success rate was 100%. The surgical techniques mainly included combined bifurcated stent grafting and embolization ( = 11), combined straight stent grafting and embolization ( = 8), and internal iliac artery (IIA) reconstruction ( = 7). There were no patient deaths or reintervention during hospitalization. The mean follow-up time was 43 months, and the patency rate of the stent was 96.2%. The overall reintervention rate was 7.7%. During the follow-up period, there were no aneurysm-related deaths. Endovascular repair involving stent graft placement, coil embolization and IIA revascularization is a safe and effective treatment for isolated CIIAA.
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http://dx.doi.org/10.3389/fsurg.2021.693233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319564PMC
July 2021

A novel homozygous mutation of the PCNT gene in a Chinese patient with microcephalic osteodysplastic primordial dwarfism type II.

Mol Genet Genomic Med 2021 Sep 31;9(9):e1761. Epub 2021 Jul 31.

Kunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Medical Center for Pediatric Diseases, Yunnan Institute of Pediatrics, Kunming Children's Hospital, Kunming, Yunnan, China.

Background: Microcephalic osteodysplastic primordial dwarfism type II (MOPD II) is a rare autosomal recessive disorder characterized by severe pre- and postnatal growth restrictions, microcephaly, skeletal dysplasia, severe teeth deformities, and typical facial features. Previous studies have shown that MOPD II is associated with mutations in the pericentrin (PCNT) gene.

Methods: We evaluated the clinical features of a 10-year and 7-month-old Chinese girl with MOPD II. Subsequently, next-generation sequencing and flow cytometry were performed to investigate genetic characteristics and the expression of PCNT protein respectively.

Results: The patient presented with short stature, microcephaly, typical craniofacial features, teeth deformity, thrombocytosis, and a delayed bone age (approximately 7 years). No abnormality in growth hormone or insulin-like growth factor 1 was detected. Notably, the patient was found to carry a novel homozygous PCNT mutation (c.6157G>T, p.Glu2053Ter), which was inherited from her healthy heterozygous parents. Meanwhile, significant deficiency of PCNT expression was identified in the patient.

Conclusion: Our study identified a novel PCNT mutation associated with MOPD II, expanded the mutation spectrum of the PCNT gene and improved our understanding of the molecular basis of MOPD II.
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http://dx.doi.org/10.1002/mgg3.1761DOI Listing
September 2021

High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.

Nat Biotechnol 2021 Jul 26. Epub 2021 Jul 26.

Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, China.

Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and the relatively low throughput of available microscopy methods. Here, we present an approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed fluorescence microscopy implementing improved volumetric imaging with synchronized on-the-fly-scan and readout technique, and is capable of completing whole-brain imaging of a rhesus monkey at 1 × 1 × 2.5 µm voxel resolution within 100 h. We also developed a highly efficient method for long-range tracing of sparse axonal fibers in datasets numbering hundreds of terabytes. This pipeline, which we call serial sectioning and clearing, three-dimensional microscopy with semiautomated reconstruction and tracing (SMART), enables effective connectome-scale mapping of large primate brains. With SMART, we were able to construct a cortical projection map of the mediodorsal nucleus of the thalamus and identify distinct turning and routing patterns of individual axons in the cortical folds while approaching their arborization destinations.
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http://dx.doi.org/10.1038/s41587-021-00986-5DOI Listing
July 2021

Oxaliplatin promotes siMAD2L2‑induced apoptosis in colon cancer cells.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.

The clinical efficacy of colorectal tumor treatment is restricted due to platinum agent resistance. Translesion DNA synthesis (TLS) has been shown to contribute to this resistance; however, the exact molecular mechanism remains unknown. The present study aimed to investigate the possible function of the core of the TLS polymerase mitotic arrest deficient 2 like 2 (MAD2L2) in drug sensitivity, in order to provide a treatment rationale for platinum‑based chemotherapy in colon cancer. In the present study, MAD2L2 was knocked down using MAD2L2‑specific small interfering (si)RNA. HCT116 and SW620 cells were treated with oxaliplatin and MG132; oxaliplatin is a platinum compound that induces DNA damage and MG132 is a potent proteasome inhibitor. Cell viability was determined using an MTT assay. Cell apoptosis was examined via flow cytometry and TUNEL assay. The activity of proteasome 26S subunit, non‑ATPase 13 (PSMD13) was detected using ELISA, while the expression levels of apoptotic‑related proteins were detected via western blotting. The results demonstrated that cells treated with oxaliplatin or MG132 alone had decreased viability, but a synergistic effect was not observed after co‑treatment. In addition, the knockdown of MAD2L2 caused by siMAD2L2 or oxaliplatin treatment increased the expression levels of the pro‑apoptotic proteins Bax and Bak and decreased the expression levels of the anti‑apoptotic protein Bcl‑2, compared with the negative control group. Moreover, MG132 alleviated the decrease in MAD2L2 expression, while reducing siMAD2L2‑induced cell apoptosis. These results indicate that oxaliplatin promotes siMAD2L2‑induced apoptosis in colon cancer cells. This process was associated with the Bcl‑2 and ubiquitin‑proteasome pathway. Overall, the present study provides a theoretical basis for improving the clinical efficacy of colon cancer by combining chemotherapy and gene therapy.
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http://dx.doi.org/10.3892/mmr.2021.12268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281267PMC
September 2021

Directional conversion of a THz propagating wave into surface waves in deformable metagratings.

Opt Express 2021 Jul;29(14):21749-21762

Controllable conversion between propagating light waves and surface waves (SWs) has recently attracted significant research interests. This paper demonstrates, via numerical simulation, for the first time all-dielectric SW converters that possess a tunable and directional SW conversion efficiency. The SW converters contain multiple metagratings of Si pillars embedded in a deformable substrate. In the analysis, an infinitely large, bi-periodic metagrating under the illumination of linearly polarized light is considered first. The SW conversion efficiency of this metagrating can be modulated between 4.3% and 51.0% for incident light frequency at 0.8 THz by stretching the deformable substrate along the direction of SW propagation. Subsequently, two SW converters under circularly polarized light illumination are analyzed, where a similar level of efficiency modulation is retained in finite-sized metagratings. In these converters, only the metagrating channels along the stretch direction have a strong SW conversion efficiency, which can reach 40.4% after normalization against the effective grating area. The directivity, a parameter defined here to reveal the energy contrast among the output channels, reaches 38.6 in one of the converters. Due to its high tunability, high directivity and compact size, the SW converters may be used as tunable optical sensors and light couplers in the THz regime.
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http://dx.doi.org/10.1364/OE.431817DOI Listing
July 2021

Genome assembly of the Chinese maize elite inbred line RP125 and its EMS mutant collection provide new resources for maize genetics research and crop improvement.

Plant J 2021 Jul 12. Epub 2021 Jul 12.

Maize Research Institute, Sichuan Agricultural University, ChengDu, 611130, China.

Maize is an important crop worldwide, as well as a valuable model with vast genetic diversity. Accurate genome and annotation information for a wide range of inbred lines would provide valuable resources for crop improvement and pan-genome characterization. In this study, we generated a high-quality de novo genome assembly (contig N50 of 15.43 Mb) of the Chinese elite inbred line RP125 using Nanopore long-read sequencing and Hi-C scaffolding, which yield highly contiguous, chromosome-length scaffolds. Global comparison of the RP125 genome with those of B73, W22, and Mo17 revealed a large number of structural variations. To create new germplasm for maize research and crop improvement, we carried out an EMS mutagenesis screen on RP125. In total, we obtained 5818 independent M2 families, with 946 mutants showing heritable phenotypes. Taking advantage of the high-quality RP125 genome, we successfully cloned 10 mutants from the EMS library, including the novel kernel mutant qk1 (quekou: "missing a small part" in Chinese), which exhibited partial loss of endosperm and a starch accumulation defect. QK1 encodes a predicted metal tolerance protein, which is specifically required for Fe transport. Increased accumulation of Fe and reactive oxygen species as well as ferroptosis-like cell death were detected in qk1 endosperm. Our study provides the community with a high-quality genome sequence and a large collection of mutant germplasm.
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http://dx.doi.org/10.1111/tpj.15421DOI Listing
July 2021

Mortalin maintains breast cancer stem cells stemness via activation of Wnt/GSK3β/β-catenin signaling pathway.

Am J Cancer Res 2021 15;11(6):2696-2716. Epub 2021 Jun 15.

The General Hospital of Ningxia Medical University Yinchuan 750004, China.

Previous research indicated that mortalin overexpressed in breast cancer and contributed to carcinogenesis. Mortalin was also demonstrated to promote Epithelial-mesenchymal transition (EMT) and was considered as a factor for maintaining the stemness of the cancer stem cells. However, the underlying mechanisms about mortalin maintaining the stemness of breast cancer stem cells (BCSCs) remain unclear. Here, we identified that increased expression of mortalin in breast cancer was associated with poorer overall survival rate. Mortalin was elevated in breast cancer cell lines and BCSC-enriched populations. Additionally, knockdown of mortalin significantly inhibited the cell proliferation, migration and EMT, as well as sphere forming capacity and stemness genes expression. Further study revealed that mortalin promoted EMT and maintained BCSCs stemness via activating the Wnt/GSK3β/β-catenin signaling pathway and . Taken together, these findings unveiled the mechanism of mortalin in maintaining and regulating the stemness of BCSCs, and may offer novel therapeutic strategies for breast cancer treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263651PMC
June 2021

BCAT1 knockdown-mediated suppression of melanoma cell proliferation and migration is associated with reduced oxidative phosphorylation.

Am J Cancer Res 2021 15;11(6):2670-2683. Epub 2021 Jun 15.

Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University Henan, China.

Malignant melanoma has a high mutational rate. As a result, resistance to current therapies is common. Consequently, there is an unmet medical need to develop novel therapies. Recent data suggest that branched-chain amino acid transaminase 1 (BCAT1) is overexpressed in multiple cancers, and such overexpressed BCAT1 is necessary for individual cancer progression. Therefore, BCAT1 appears to be a good target in cancer treatment. Additionally, because its expression in healthy tissues is highly restricted in adults and is limited to the brain, ovary, and placenta, BCAT1 is especially an ideal target in cancer therapies. Currently, the function of BCAT1 in malignant melanoma has not been demonstrated. Therefore, we investigated the role of BCAT1 in the proliferation and migration of malignant melanomas using human samples and mouse malignant B16 melanoma cell line. Our data showed that BCAT1 was overexpressed in malignant melanoma tissues both in humans and mice. Besides, BCAT1 knockdown suppressed melanoma cell proliferation and migration, which was associated with reduced oxidative phosphorylation. Collectively, our data indicate that BCAT1 is a promising therapeutic target for the treatment of malignant melanomas.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263658PMC
June 2021

Luteolin activates Tregs to promote IL-10 expression and alleviating caspase-11-dependent pyroptosis in sepsis-induced lung injury.

Int Immunopharmacol 2021 Oct 8;99:107914. Epub 2021 Jul 8.

Department of Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Objectives: Acute respiratory distress syndrome (ARDS) is characterized by an excessive pulmonary inflammatory response. Pyroptosis is a newly form of programmed inflammatory cell death that is triggered by inflammatory caspases. Studies have shown that Luteolin has powerful anti-inflammation effects through activating the function of regulatory T cells (Tregs). The study aimed at investigating the effects of Luteolin on CLP-induced ALI.

Methods: In our study, we employed the mouse cecal ligation and puncture (CLP) model to explore whether Luteolin contributed to alleviated lung injury in vivo. H&E staining and wet/dry (W/D) weight ratios were used to evaluate the severity of lung injury. The serum and BALF of cytokines were assessed by ELISA. The number of neutrophils in the BALF was counted. Immunohistochemistry of IL-10 and MPO in lung tissue was detected. The ROS level in lung was tested by ROS Assay Kit and expression of Gpx4 in lung tissue was detected by qRT-PCR and Western blotting. The regulatory T cells (Treg) population was analyzed in spleen and Peripheral blood mononuclear cells (PBMCs). The levels of caspase-11 protein, caspase-1 protein, GSDMD protein, IL-1α and IL-1β protein in the lung tissue was evaluated by Western blotting.

Results: We found Luteolin significantly inhibits inflammation and attenuated CLP-induced lung injury in vivo, and the levels of, caspase-11, caspase-1, GSDMD, IL-1α and IL-1β protein in the lungs of CLP mice decreased significantly after pretreatment with Luteolin. Furthermore, the results showed that Luteolin could increase Treg frequencies and IL-10 levels in serum and BALF of CLP mice. It is noteworthy that depleting Tregs reverse Luteolin ameliorated lung injury, and IL-10 neutralizing antibodies treatment aggravated lung pyroptosis.

Conclusions: Our study illustrated that Luteolin contributed to alleviated lung injury, and attenuated caspase-11-dependent pyroptosis in the lung tissue of the CLP-induced ALI mouse model. The mechanisms could be related to regulating the frequency of Tregs and the levels of Treg derived IL-10. Treg cells were show to produce IL-10 and could alleviating caspase-11-dependent lung pyroptosis.
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http://dx.doi.org/10.1016/j.intimp.2021.107914DOI Listing
October 2021

Influences Gasdermin-D-Dependent Pyroptosis of the Lung via Regulating Toll-Like Receptor 2-Mediated Regulatory T Cell Differentiation.

J Immunol Res 2021 14;2021:5538612. Epub 2021 Jun 14.

Department of Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: , as an opportunistic fungus, has developed a series of escape mechanisms under the host's immune response to obtain nutrients and promote fungal growth in the hostile environment. The immune escape of pathogens may be through suppressing the inflammatory response mediated by regulatory T cells (Tregs). The aim of this study was to explore whether influences Gasdermin-D-dependent pyroptosis of the lung by regulating Toll-like receptor 2-mediated regulatory T cell differentiation.

Methods: Collect peripheral blood from patients with . ELISA kits we used to detect the expression levels of IL-1, IL-6, IL-2R, and IL-10 in the serum and flow cytometry to detect the percentage of CD4CD25Foxp3 Tregs in the patients' peripheral blood mononuclear cells (PBMCs). The mouse model of infection was constructed by tracheal instillation. The pathological changes in the lungs of the mice were observed under a microscope. The fungal load in the lung tissue was determined by the plate colony count. ELISA kit was used to detect the lung tissue homogenate proinflammatory cytokines TNF-, IL-6, CCL2, and VEGF. Q-PCR was used for the detection of the expression of Foxp3 and TLR2 genes in the lung. Western blot was used for the detection of the expression of TLR2, Gasdermin-D (GSDMD), IL-1, and IL-1 in the lung. Flow cytometry was used to detect splenic CD4CD25FOXP3 Tregs. Using magnetic beads to extract CD4 T cells from mice spleen, the effects of conidia or TLR2 inhibitor (C29) to differentiate CD4 T cells in vitro were tested.

Results: The expression of Foxp3 and TLR2 in the lung tissue of mice infected with increased, and we observed that the proportion of Tregs in both infection patients and mice was upregulated. After using the CD25 neutralizing antibody, the number of Tregs in the mice spleen was significantly reduced. However, lung damage was reduced and the ability to clear lung fungi was enhanced. We found that the Tregs in TLR2 mice were significantly reduced and the nonlethal dose of conidia did not cause severe lung damage in TLR2 mice. Compared with that of wild-type mice, the fungal burden in the lung of TLR2-deficient mice was reduced and the knockout of TLR2 changed the expression of GSDMD, IL-1, and IL-1 in . In in vitro experiments, we found that the inhibition of TLR2 can reduce Treg differentiation.

Conclusions: triggers CD4CD25FOXP3 Treg proliferation and differentiation by activating the TLR2 pathway, which may be a potential mechanism for evading host defenses in . This effect can modulate GSDMD-dependent pyroptosis and may partly involve TRL2 signaling.
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http://dx.doi.org/10.1155/2021/5538612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219420PMC
June 2021

A Kalman Filter-Based Kernelized Correlation Filter Algorithm for Pose Measurement of a Micro-Robot.

Micromachines (Basel) 2021 Jun 30;12(7). Epub 2021 Jun 30.

Mechanical Engineering College, Zhejiang University of Technology, Hangzhou 310023, China.

This paper proposes a moving-target tracking algorithm that measures the pose of a micro-robot with high precision and high speed using the Kalman filter-based kernelized correlation filter (K2CF) algorithm. The adaptive Kalman filter can predict the state of linearly and nonlinearly fast-moving targets. The kernelized correlation filter algorithm then accurately detects the positions of the moving targets and uses the detection results to modify the moving states of the targets. This paper verifies the performance of the algorithm on a monocular vision measurement platform and using a pose measurement method. The K2CF algorithm was embedded in the micro-robot's attitude measurement system, and the tracking performances of three different trackers were compared under different motion conditions. Our tracker improved the positioning accuracy and maintained real-time operation. In a comparison study of K2CF and many other algorithms on Object Tracking Benchmark-50 and Object Tracking Benchmark-100 video sequences, the K2CF algorithm achieved the highest accuracy. In the 400 mm × 300 mm field of view, when the target radius is about 3 mm and the inter-frame acceleration displacement does not exceed 5.6 mm, the root-mean-square error of position and attitude angle can satisfy the precision requirements of the system.
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http://dx.doi.org/10.3390/mi12070774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306500PMC
June 2021

Machine learning for MRI radiomics: a study predicting tumor-infiltrating lymphocytes in patients with pancreatic ductal adenocarcinoma.

Abdom Radiol (NY) 2021 10 29;46(10):4800-4816. Epub 2021 Jun 29.

Department of Radiology, Changhai Hospital, Navy Medical University, Shanghai, 200434, China.

Objective: To develop and validate a machine learning classifier based on magnetic resonance imaging (MRI), for the preoperative prediction of tumor-infiltrating lymphocytes (TILs) in patients with pancreatic ductal adenocarcinoma (PDAC).

Materials And Methods: In this retrospective study, 156 patients with PDAC underwent MR scan and surgical resection. The expression of CD4, CD8 and CD20 was detected and quantified using immunohistochemistry, and TILs score was achieved by Cox regression model. All patients were divided into TILs score-low and TILs score-high groups. The least absolute shrinkage and selection operator method and the extreme gradient boosting (XGBoost) were used to select the features and to construct a prediction model. The performance of the models was assessed using the training cohort (116 patients) and the validation cohort (40 patients), and decision curve analysis (DCA) was applied for clinical use.

Results: The XGBoost prediction model showed good discrimination in the training (AUC 0.86; 95% CI 0.79-0.93) and validation sets (AUC 0.79; 95% CI 0.64-0.93). The sensitivity, specificity, and accuracy for the training set were 86.67%, 75.00%, and 0.81, respectively, whereas those for the validation set were 84.21%, 66.67%, and 0.75, respectively. Decision curve analysis indicated the clinical usefulness of the XGBoost classifier.

Conclusion: The model constructed by XGBoost could predict PDAC TILs and may aid clinical decision making for immune therapy.
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http://dx.doi.org/10.1007/s00261-021-03159-9DOI Listing
October 2021

Targeting microRNAs to Regulate the Integrity of the Blood-Brain Barrier.

Front Bioeng Biotechnol 2021 11;9:673415. Epub 2021 Jun 11.

Centre for Motor Neuron Disease, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia.

The blood-brain barrier (BBB) is a highly specialized neurovascular unit that protects the brain from potentially harmful substances. In addition, the BBB also engages in the exchange of essential nutrients between the vasculature and brain parenchyma, which is critical for brain homeostasis. Brain diseases, including neurological disorders and cerebrovascular diseases, are often associated with disrupted BBB integrity, evidenced by increased permeability. Therefore, defining the mechanisms underlying the regulation of BBB integrity is crucial for the development of novel therapeutics targeting brain diseases. MicroRNAs (miRNA), a type of small non-coding RNAs, are emerging as an important regulator of BBB integrity. Here we review recent developments related to the role of miRNAs in regulating BBB integrity.
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http://dx.doi.org/10.3389/fbioe.2021.673415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226126PMC
June 2021

Preoperative Prediction of G1 and G2/3 Grades in Patients With Nonfunctional Pancreatic Neuroendocrine Tumors Using Multimodality Imaging.

Acad Radiol 2021 Jun 23. Epub 2021 Jun 23.

Department of Radiology, Changhai Hospital, Naval Medical University, Changhai road 168, Shanghai 200434, China. Electronic address:

Objectives: We aimed to develop and validate a multimodality radiomics model for the preoperative prediction of nonfunctional pancreatic neuroendocrine tumor (NF-pNET) grade (G).

Methods: This retrospective study assessed 123 patients with surgically resected, pathologically confirmed NF-pNETs who underwent multidetector computed tomography and MRI scans between December 2012 and May 2020. Radiomic features were extracted from multidetector computed tomography and MRI. Wilcoxon rank-sum test and Max-Relevance and Min-Redundancy tests were used to select the features. The linear discriminative analysis (LDA) was used to construct the four models including a clinical model, MRI radiomics model, computed tomography radiomics model, and mixed radiomics model. The performance of the models was assessed using a training cohort (82 patients) and a validation cohort (41 patients), and decision curve analysis was applied for clinical use.

Results: We successfully constructed 4 models to predict the tumor grade of NF- pNETs. Model 4 combined 6 features of T2-weighted imaging radiomics features and 1 arterial-phase computed tomography radiomics feature, and showed better discrimination in the training cohort (AUC = 0.92) and validation cohort (AUC = 0.85) relative to the other models. In the decision curves, if the threshold probability was 0.07-0.87, the use of the radiomics score to distinguish NF-pNET G1 and G2/3 offered more benefit than did the use of a "treat all patients" or a "treat none" scheme in the training cohort of the MRI radiomics model.

Conclusion: The LDA classifier combining multimodality images may be a valuable noninvasive tool for distinguishing NF-pNET grades and avoid unnecessary surgery.
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http://dx.doi.org/10.1016/j.acra.2021.05.017DOI Listing
June 2021
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