Publications by authors named "Fang Teng"

48 Publications

The Regulation Network and Clinical Significance of Circular RNAs in Breast Cancer.

Front Oncol 2021 9;11:691317. Epub 2021 Jul 9.

Deparment of Gynecology, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, China.

Breast cancer is one of the most common malignant tumors in women worldwide. Circular RNA (circRNA) is a class of structurally stable non-coding RNA with a covalently closed circular structure. In recent years, with the development of high-throughput RNA sequencing, many circRNAs have been discovered and have proven to be clinically significant in the development and progression of breast cancer. Importantly, several regulators of circRNA biogenesis have been discovered. Here, we systematically summarize recent progress regarding the network of regulation governing the biogenesis, degradation, and distribution of circRNAs, and we comprehensively analyze the functions, mechanisms, and clinical significance of circRNA in breast cancer.
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http://dx.doi.org/10.3389/fonc.2021.691317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299466PMC
July 2021

A review of regulated self-organizing approaches for tissue regeneration.

Prog Biophys Mol Biol 2021 Jul 19. Epub 2021 Jul 19.

Department of Gynaecology and Obstetrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, 210004, China. Electronic address:

Tissue and organ regeneration is the dynamic process by which a population of cells rearranges into a specific form with specific functions. Traditional tissue regeneration utilizes tissue grafting, cell implantation, and structured scaffolds to achieve clinical efficacy. However, tissue grafting methods face a shortage of donor tissue, while cell implantation may involve leakage of the implanted cells without a supportive 3D matrix. Cell migration, proliferation, and differentiation in structured scaffolds may disorganize and frustrate the artificially pre-designed structures, and sometimes involve immunogenic reactions. To overcome this limitation, the self-organizing properties and innate regenerative capability of tissue/organism formation in the absence of guidance by structured scaffolds has been investigated. This review emphasizes the growing subfield of the regulated self-organizing approach for neotissue formation and describes advances in the subfield using diverse, cutting-edge, inter-disciplinarity technologies. We cohesively summarize the directed self-organization of cells in the micro-engineered cell-ECM system and 3D/4D cell printing. Mathematical modeling of cellular self-organization is also discussed for providing rational guidance to intractable problems in tissue regeneration. It is envisioned that future self-organization approaches integrating biomathematics, micro-nano engineering, and gene circuits developed from synthetic biology will continue to work in concert with self-organizing morphogenesis to enhance rational control during self-organizing in tissue and organ regeneration.
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http://dx.doi.org/10.1016/j.pbiomolbio.2021.07.006DOI Listing
July 2021

Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix-Receptor Interaction Pathway.

Cancer Biother Radiopharm 2021 Jul 8. Epub 2021 Jul 8.

Department of Gynecology, Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing, China.

The authors' previous study showed that the long noncoding RNA CTD-2589M5.4 was significantly upregulated in multidrug-resistant ovarian cancer cells. However, the role of CTD-2589M5.4 in the progression of ovarian cancer remains unclear. The purpose of this current study was to illuminate the biological function and possible mechanism of CTD-2589M5.4 in ovarian cancer development. The expression of CTD-2589M5.4 was examined via real-time quantitative PCR in primary ovarian cancer tissues (POCTs) and ovarian cancer cell lines. The biological function of CTD-2589M5.4 was analyzed via CCK-8 proliferation, wound healing, transwell, and flow cytometry assays in CTD-2589M5.4-overexpressed/silenced and control ovarian cancer cells. The mechanism of CTD-2589M5.4 function in ovarian cancer progression was analyzed utilizing high-throughput RNA-sequencing, Kyoto Encyclopedia of Genes and Genomes analysis, qRT-PCR, western blot, and rescue experiments. CTD-2589M5.4 expression was decreased in the POCTs and ovarian cancer cells compared with the normal ovarian tissues ( < 0.05) and normal ovarian epithelial cells ( < 0.05). Overexpression of CTD-2589M5.4 inhibited the proliferation, invasion, and migration of ovarian cancer cells, while knockdown of CTD-2589M5.4 had the opposite effect. Furthermore, a total of 750 and 233 genes were notably upregulated and downregulated, respectively, in the CTD-2589M5.4-overexpressed A2780 cells, while the extracellular matrix (ECM)-receptor interaction pathway was significantly downregulated. In addition, overexpression of fibronectin 1 significantly abrogated the tumor suppressive function of CTD-2589M5.4. This study demonstrated that CTD-2589M5.4 could inhibit ovarian cancer cell proliferation, invasion, and migration, at least partially by way of downregulating the ECM-receptor interaction pathway, therefore providing a potential therapeutic target for the prevention and/or treatment of ovarian cancer.
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http://dx.doi.org/10.1089/cbr.2020.4429DOI Listing
July 2021

Effect of PDCA-based nursing management model on the quality of life and complications of patients with acute leukemia undergoing chemotherapy.

Am J Transl Res 2021 15;13(4):3246-3253. Epub 2021 Apr 15.

Department of Emergency, Affiliated Hospital of Jiangnan University Wuxi, Jiangsu Province, China.

Objective: To assess the influence of PDCA-based nursing management model on the quality of life (QOL) and complications of patients with acute leukemia undergoing chemotherapy.

Methods: In this study, we randomly divided 118 patients with acute leukemia undergoing chemotherapy in our hospital into an observation group (n=59) and a control group (n=59). The control group was implemented routine nursing while the observation group was implemented PDCA-based nursing management. The anxiety, depression, QOL, cancer fatigue, total complication rate, sleep quality, and patients' satisfaction were compared between the two groups.

Results: The SAS and SDS scores of the observation group were significantly lower than those of the control group (P<0.05). The scores of physical function, general health, social function, emotional role, and mental health of the observation group were superior to those of the control group (all P<0.05). The 4 dimensions of behavior/severity, sense, emotion and cognition/mood in the observation group were significantly lower than those of the control group (all P<0.05). The incidence of complications such as nausea and vomiting, infection, bleeding, phlebitis, bone marrow suppression and mucositis in the observation group was significantly lower than that in the control group (P<0.05). The scores of sleep quality in the observation group were significantly lower than those of the control group (all P<0.05). The observation group's satisfaction with PDCA-based nursing management was markedly higher than that of the control group (P<0.05).

Conclusion: The PDCA-based nursing management model applied to patients with acute leukemia undergoing chemotherapy has shown good effects, which can improve depression, anxiety, cancer fatigue, sleep quality and QOL. It also significantly reduced the incidence of complications and improved patients' satisfaction, which was worth of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129232PMC
April 2021

Pathogenic Heteroplasmic Somatic Mitochondrial DNA Mutation Confers Platinum-Resistance and Recurrence of High-Grade Serous Ovarian Cancer.

Cancer Manag Res 2020 2;12:11085-11093. Epub 2020 Nov 2.

Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, People's Republic of China.

Purpose: Platinum resistance is a primary barrier to improving the survival rate of ovarian cancer. The relationship between mtDNA somatic mutations and response to platinum-based chemotherapy in ovarian cancer has not been well clarified.

Patients And Methods: Here, we employed the next-generation sequencing (NGS) platform to identify mtDNA mutations of the unrelated high-grade serous ovarian cancer (HGSOC) patients.

Results: We identified 569 germline variants and 28 mtDNA somatic mutations, and found the platinum-sensitive relapsed HGSOC patients had more synonymous mutations while the platinum-resistant relapsed HGSOC patients had more missense mutations in the mtDNA somatic mutations. Meanwhile, we found that the HGSOC patients who harbored heteroplasmic pathogenic mtDNA somatic mutations had significantly higher prevalence of both platinum-resistance and relapse than those without (80.0% versus 16.7%, p=0.035). Additionally, we observed that the tumor tissues had significantly higher lactate-to-pyruvate (L/P) ratio than the paired nontumor tissues (p<0.001), and L/P ratio of tumors with any heteroplasmic pathogenic mtDNA mutations was significantly higher than that of the tumors free of pathogenic mtDNA mutations (p=0.025).

Conclusion: Our findings indicate that these heteroplasmic pathogenic mtDNA somatic mutations may cause decreased respiratory chain activity and lead to the metabolism remodeling that seem to be beneficial for progression of both platinum-based chemotherapy resistance and relapse.
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http://dx.doi.org/10.2147/CMAR.S277724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646460PMC
November 2020

Comparison of tendon suture fixation and cortical screw fixation for treatment of distal tibiofibular syndesmosis injury: A case-control study.

Medicine (Baltimore) 2020 Aug;99(34):e21573

People's Hospital of Pengzhou City, Sichuan Province, People's Republic of China.

This study aimed to investigate the effectiveness of tendon suture fixation versus cortical screw fixation for the treatment of distal tibiofibular syndesmosis injury.This study recruited 42 patients with Danis-Weber type B, C1 and C2 fractures concomitant with lower tibiofibular syndesmosis injury, who were randomly assigned to 2 groups according to treatment with cortical screw fixation (n = 21) and tendon suture fixation (n = 21). Operation time, intraoperative blood loss, time to full weight-bearing activity, medical cost, ankle function, and ankle pain were compared between the 2 groups.The operation time was significantly less with cortical screw fixation (57.1 ± 5.3 min) than with tendon suture fixation (63.3 ± 6.3 min; p = 0.01), but there was no significant difference in intraoperative blood loss. The time until full weight-bearing was possible was significantly longer after cortical screw fixation (10.9 ± 2.7 weeks) than after tendon suture fixation (7.1 ± 1.9 weeks; P < .001). The medical cost was much greater for cortical screw fixation (1861.6 ± 187.3 USD) than for tendon suture fixation (1209.6 ± 97.6 USD; P < .01). The rate of excellent and good ankle function at 3 months after surgery was significantly higher with tendon suture fixation (71.4%) than with cortical screw fixation (33.3%; P = .03).Tendon suture fixation is associated with quicker recovery of ankle function, shorter time to full weight-bearing, and lower medical cost to the patient compared with screw fixation. Our findings suggest that tendon suture fixation is an effective method for the treatment of tibiofibular syndesmosis injury.
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http://dx.doi.org/10.1097/MD.0000000000021573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447365PMC
August 2020

Which sample type is better for Xpert MTB/RIF to diagnose adult and pediatric pulmonary tuberculosis?

Biosci Rep 2020 08;40(8)

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: This review aimed to identify proper respiratory-related sample types for adult and pediatric pulmonary tuberculosis (PTB), respectively, by comparing performance of Xpert MTB/RIF when using bronchoalveolar lavage (BAL), induced sputum (IS), expectorated sputum (ES), nasopharyngeal aspirates (NPAs), and gastric aspiration (GA) as sample.

Methods: Articles were searched in Web of Science, PubMed, and Ovid from inception up to 29 June 2020. Pooled sensitivity and specificity were calculated, each with a 95% confidence interval (CI). Quality assessment and heterogeneity evaluation across included studies were performed.

Results: A total of 50 articles were included. The respective sensitivity and specificity were 87% (95% CI: 0.84-0.89), 91% (95% CI: 0.90-0.92) and 95% (95% CI: 0.93-0.97) in the adult BAL group; 90% (95% CI: 0.88-0.91), 98% (95% CI: 0.97-0.98) and 97% (95% CI: 0.95-0.99) in the adult ES group; 86% (95% CI: 0.84-0.89) and 97% (95% CI: 0.96-0.98) in the adult IS group. Xpert MTB/RIF showed the sensitivity and specificity of 14% (95% CI: 0.10-0.19) and 99% (95% CI: 0.97-1.00) in the pediatric ES group; 80% (95% CI: 0.72-0.87) and 94% (95% CI: 0.92-0.95) in the pediatric GA group; 67% (95% CI: 0.62-0.72) and 99% (95% CI: 0.98-0.99) in the pediatric IS group; and 54% (95% CI: 0.43-0.64) and 99% (95% CI: 0.97-0.99) in the pediatric NPA group. The heterogeneity across included studies was deemed acceptable.

Conclusion: Considering diagnostic accuracy, cost and sampling process, ES was a better choice than other sample types for diagnosing adult PTB, especially HIV-associated PTB. GA might be more suitable than other sample types for diagnosing pediatric PTB. The actual choice of sample types should also consider the needs of specific situations.
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http://dx.doi.org/10.1042/BSR20200308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403955PMC
August 2020

Tuning Optimum Temperature Range of Bi Te -Based Thermoelectric Materials by Defect Engineering.

Chem Asian J 2020 Sep 7;15(18):2775-2792. Epub 2020 Aug 7.

State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Bi Te -based solid solutions, which have been widely used as thermoelectric (TE) materials for the room temperature TE refrigeration, are also the potential candidates for the power generators with medium and low-temperature heat sources. Therefore, depending on the applications, Bi Te -based materials are expected to exhibit excellent TE properties in different temperature ranges. Manipulating the point defects in Bi Te -based materials is an effective and important method to realize this purpose. In this review, we focus on how to optimize the TE properties of Bi Te -based TE materials in different temperature ranges by defect engineering. Our calculation results of two-band model revel that tuning the carrier concentration and band gap, which is easily realized by defects engineering, can obtain better TE properties at different temperatures. Then, the typical paradigms about optimizing the TE properties at different temperatures for n-type and p-type Bi Te -based ZM ingots and polycrystals are discussed in the perspective of defects engineering. This review can provide the guidance to improve the TE properties of Bi Te -based materials at different temperatures by defects engineering.
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http://dx.doi.org/10.1002/asia.202000793DOI Listing
September 2020

Single-Cell Multimodal Analytical Approach by Integrating Raman Optical Tweezers and RNA Sequencing.

Anal Chem 2020 08 23;92(15):10433-10441. Epub 2020 Jul 23.

Single-cell analysis has become a state-of-art approach to heterogeneity profiling in tumor cells. Herein, we realize a kind of single-cell multimodal analytical approach by combining single-cell RNA sequencing (scRNA-seq) with Raman optical tweezers (ROT), a label-free single-cell identification and isolation technique, and apply it to investigate drug sensitivity. The drug sensitivity of human BGC823 gastric cancer cells toward different drugs, paclitaxel and sodium dichloroacetate, was distinguished in the conjoint analytical way including morphology monitoring, Raman identification, and transcriptomic profiling. Each individual BGC823 cancer cell was measured by Raman spectroscopy, then nondestructively isolated out by ROT, and finally RNA-sequenced. Our results demonstrate each analytical mode can reflect cell response to the drugs from different perspectives and is consistent and complementary with each other. Therefore, we believe the multimodal analytical approach offers an access to comprehensive characterizations of the unicellular complexity, which especially makes sense for studying tumor heterogeneity or a desired special cell from a mixture cell sample such as whole blood.
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http://dx.doi.org/10.1021/acs.analchem.0c00912DOI Listing
August 2020

Rapid and accurate identification of marine microbes with single-cell Raman spectroscopy.

Analyst 2020 May 19;145(9):3297-3305. Epub 2020 Mar 19.

Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, School of Electronics Engineering and Computer Science, Peking University, No. 5 Yiheyuan Road, Beijing, P. R. China.

Rapid and accurate identification of individual microorganisms, such as pathogenic or unculturable microbes, is significant in microbiology. In this work, rapid identification of marine microorganisms by single-cell Raman spectroscopy (scRS) using one-dimensional convolutional neural networks (1DCNN) was explored. Here, single-cell Raman spectra of ten species of marine actinomycetes, two species of non-marine actinomycetes and E. coli (as a reference) were individually collected. Several common classification algorithms in chemometrics, including linear discriminant analysis with principal component analysis and a support vector machine, were applied to evaluate the 1DCNN performance based on the raw and pre-processed Raman spectra. 1DCNN showed superior performance on the raw data in terms of its accuracy and recall rate compared with other classification algorithms. Our investigation demonstrated that the scRS-integrating advanced 1DCNN classification algorithm provided a rapid and accurate approach for identifying individual microorganisms without time-consuming cell culture and sophisticated or specific techniques, which could be a useful methodology for discriminating the microbes that cannot be cultured under normal conditions, especially for 'biological risk'-related emergencies.
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http://dx.doi.org/10.1039/c9an02069aDOI Listing
May 2020

Effect of the Soluble Factors Released by Dental Apical Papilla-Derived Stem Cells on the Osteo/Odontogenic, Angiogenic, and Neurogenic Differentiation of Dental Pulp Cells.

Stem Cells Dev 2020 06 27;29(12):795-805. Epub 2020 Apr 27.

Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.

Stem cells derived from dental apical papilla (SCAPs) can secrete various soluble factors, which may stimulate tissue repair and regeneration in vivo. The aim of this study was to elucidate the effect of the soluble factors released by SCAPs on the proliferation and differentiation of dental pulp cells (DPCs). We compared the osteo/odontogenic, angiogenic, and neurogenic effects of soluble factors released from SCAPs and bone marrow mesenchymal stem cells (BMSCs) in vitro. Conditioned media (CM) were collected from human SCAPs and BMSCs cultures, and their effects on human DPCs proliferation and differentiation were evaluated. Cellular proliferation was unaffected by SCAPs-CM and was inhibited by BMSCs-CM. Cells treated with osteo/odontogenic inducing medium (OM) plus SCAPs-CM showed higher alkaline phosphatase activity than did cells in the OM group. The expression level of osteo/odontogenic markers were higher in the SCAPs-CM plus OM group than in the BMSCs-CM plus OM and OM groups. SCAPs-CM and BMSCs-CM significantly promoted DPCs migration. DPCs angiogenic differentiation was not affected by SCAPs-CM but was significantly enhanced by BMSCs-CM. In DPCs cultured in media optimized for neural stem cell growth for 2 weeks, the expression levels of neurogenic markers were significantly enhanced by the addition of SCAPs-CM. Neuronal markers expression was significantly reduced, while neurotrophic marker expression significantly increased by the addition of BMSCs-CM. In conclusion, SCAPs-CM significantly enhanced osteo/odontogenic differentiation, migration, and neurogenic differentiation potential of DPCs, but have no effect on DPCs proliferation and angiogenic differentiation in vitro. CM released from SCAPs have a greater osteo/odontogenic and neurogenic inductive effect on DPCs than BMSCs-CM. It indicates that SCAPs-CM can serve as additive to improve pulp tissue repair and regeneration.
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http://dx.doi.org/10.1089/scd.2019.0262DOI Listing
June 2020

A New Risk Index Combining d-Dimer, Fibrinogen, HE4, and CA199 Differentiates Suspecting Endometrial Cancer From Patients With Abnormal Vaginal Bleeding or Discharge.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033819901117

Department of Obstetrics and Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

Purpose: To establish an efficient new risk index for screening patients with endometrial cancer from patients with abnormal vaginal bleeding or discharge.

Method: A total of 254 patients with abnormal vaginal bleeding or discharge were included in this study. Several candidate markers, including HE4, CA125, CA199, CA153, AFP, CEA, d-dimer, and fibrinogen, were employed. A new risk index for endometrial cancer screening was established by binary logistic regression. The diagnostic value of the candidate markers and the new risk index were assessed by a receiver operating characteristic curve, sensitivity, and specificity.

Results: The most valuable diagnostic indicator for endometrial cancer was HE4, followed by d-dimer and then fibrinogen (area under the receiver operating characteristic curve: HE4 = 0.794, d-dimer = 0.717, fibrinogen = 0.690). The new risk index was superior to a single application of markers and a widely used combination (HE4 and CA125). At the ideal cutoff level, the sensitivity and specificity were 91.34% and 70.08%, respectively. In addition, only patients without organic disease served as controls, which further increase its performance (area under the receiver operating characteristic curve = 0.932, sensitivity = 94.49%, and specificity = 77.42%).

Conclusions: The new risk index combining HE4, d-dimer, fibrinogen, and CA199 was the ideal combination for the screening of endometrial cancer. As a simple, rapid, nondestructive detection method, the new risk index is worth promotion in clinical practice, especially in primary medical institutions.
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http://dx.doi.org/10.1177/1533033819901117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074482PMC
September 2020

Retraction notice to "miR-149 regulates the proliferation and apoptosis of cervical canc er cells by targeting GIT1" [Biomed. Pharmacother. 105 (2018) 1106-1116].

Biomed Pharmacother 2020 05 16;125:109971. Epub 2020 Feb 16.

Department of Gyneology, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, China. Electronic address:

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http://dx.doi.org/10.1016/j.biopha.2020.109971DOI Listing
May 2020

[Therapeatic Efficacy of Decitabine on Low and Moderate-Risk MDS Patients and the Prognostic Factors].

Authors:
Hua-E Shu Teng Fang

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Feb;28(1):214-217

Department of Hematology, The People's Hospital of Chongqing Kaizhou District, Chongqing 405400, China,E-mail:

Objective: To investigate the efficacy of decitabine in the treatment of patients with low-risk and moderate-risk myelodysplastic syndrome (MDS) and to analyze the prognostic factors.

Methods: A retrospective study was conducted on 47 patients with low and moderate risk MDS treated with decitabine in our hospital. The course of treatment was 20 mg/(m·d)×5 d injected subcutaneously for 28 d as a course of treatment. After 3 to 4 courses of treatment, the therapeutic response and survival prognostic factors of patients were evaluated.

Results: According to the revised IWG standard, 18 patients (38.3%) achieved CR, 13 patients (27.7%) achieved PR, 7 patients (14.9%) showed hematological improvement, 7 patients (14.9%) died of pulmonary infection or myelosuppression, 2 patients transformed into acute myeloid leukemia. The median survival time of MDS patients was 26 months. The overall effective rate of decitabine regimen was 80.9% (38/47). The independent poor prognostic factors for survival were high IPSS grade, long course of MDS, early treatment for MDS, and elder.

Conclusion: The long-term efficacy of decitabine in the treatment of low and moderate-risk MDS is definite. The clinical factors before treatment may predict the efficacy of decitabine in the treatment of MDS.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.01.036DOI Listing
February 2020

Evolution of the Intrinsic Point Defects in Bismuth Telluride-Based Thermoelectric Materials.

ACS Appl Mater Interfaces 2019 Nov 28;11(44):41424-41431. Epub 2019 Oct 28.

State Key Laboratory of Silicon Materials, and School of Materials Science and Engineering , Zhejiang University , Hangzhou 310027 , China.

In polycrystalline bismuth telluride-based thermoelectric materials, mechanical-deformation-induced donor-like effects can introduce a high concentration of electrons to change the thermoelectric properties through the evolution of intrinsic point defects. However, the evolution law of these point defects during sample preparation remains elusive. Herein, we systematically investigate the evolution of intrinsic point defects in -type BiTe-based materials from the perspective of thermodynamics and kinetics, in combination with positron annihilation measurement. It is found that not only the mechanical deformation but also the sintering temperature is vital to the donor-like effect. The mechanical deformation can promote the formation of cation vacancies and facilitate the donor-like effect, and the sintering process can provide excess energy for Bi antisite atoms to surmount the diffusion potential barrier. This work provides us a better understanding of the evolution law of intrinsic point defects in BiTe-based alloys and guides us to control the carrier concentration by manipulating intrinsic point defects.
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http://dx.doi.org/10.1021/acsami.9b15198DOI Listing
November 2019

Nondestructive Identification and Accurate Isolation of Single Cells through a Chip with Raman Optical Tweezers.

Anal Chem 2019 08 10;91(15):9932-9939. Epub 2019 Jul 10.

Raman optical tweezers (ROT) as a label-free technique plays an important role in single-cell study such as heterogeneity of tumor and microbial cells. Herein we designed a chip utilizing ROT to isolate a specific single cell. The chip was made from a polydimethylsiloxane (PDMS) slab and formed into a gourd-shaped reservoir with a connected channel on a cover glass. On the chip an individual cell could be isolated from a cell crowd and then extracted with ∼0.5 μL of phosphate-buffered saline (PBS) via pipet immediately after Raman spectral measurements of the same cell. As verification, we separated four different type of cells including BGC823 gastric cancer cells, erythrocytes, lymphocytes, and cells and quantifiably characterized the heterogeneity of the cancer cells, leukocyte subtype, and erythrocyte status, respectively. The average time of identifying and isolating a specific cell was 3 min. Cell morphology comparison and viability tests showed that the successful rate of single-cell isolation was about 90%. Thus, we believe our platform could further couple other single-cell techniques such as single-cell sequencing and become a multiperspective analytical approach at the level of a single cell.
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http://dx.doi.org/10.1021/acs.analchem.9b01604DOI Listing
August 2019

Comprehensive circular RNA expression profiles and the tumor-suppressive function of circHIPK3 in ovarian cancer.

Int J Biochem Cell Biol 2019 07 23;112:8-17. Epub 2019 Apr 23.

Department of Gynecology, Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing 210004, China. Electronic address:

Background: With the development of next-generation sequencing (NGS), thousands of circular RNAs (circRNAs) have been found. Many circRNAs have been verified to play vital roles in carcinogenesis. However, whether circRNAs engage in the development and progression of ovarian cancer remains to be clarified.

Methods: We analyzed circRNA expression profiling in epithelial ovarian cancer (EOC) and normal ovarian tissues (NOT) using NGS and validated six randomly selected circRNAs via quantitative real-time-PCR (qRT-PCR), reverse-transcription PCR (RT-PCR) and Sanger sequencing after RNase treatment. CircHIPK3, the most abundant circRNA in our sequencing data, was further knocked down by siRNA. The circHIPK3 function in proliferation, invasion, migration and apoptosis of ovarian cancer cells and normal ovarian epithelial cells was analyzed via cell counting-kit 8 (CCK8), wound healing, transwell and flow cytometry analyses after circHIPK3 was efficiently silenced.

Results: Altogether, we found 7333 circRNAs, of which 4505 (61.43%) were newly identified, 2431 were significantly upregulated and 3120 were remarkably downregulated. Six randomly selected differentially expressed circRNAs were examined in 18 EOC and 18 NOT. Furthermore, the results of RT-PCR and Sanger sequencing after RNase treatment confirmed head-to-tail back-splicing. Silencing of circHIPK3 promoted proliferation, migration, and invasion and inhibited apoptosis of ovarian cancer cells (A2780 and SKOV3) and normal ovarian epithelial cells (IOSE80). Additionally, the circHIPK3-miRNA-mRNA axis was predicted as the possible mechanism using bioinformatic approaches.

Conclusions: We identified the circRNA expression profile in ovarian cancer tissues and further verified the existence and expression of six randomly selected differentially expressed circRNAs. Besides, we also found that circHIPK3 is an important regulator of ovarian cancer progression.
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http://dx.doi.org/10.1016/j.biocel.2019.04.011DOI Listing
July 2019

Mass spectrometry-based peptidome profiling of human serous ovarian cancer tissues.

Int J Biochem Cell Biol 2019 02 11;107:53-61. Epub 2018 Dec 11.

Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing, 210004, China. Electronic address:

Background: Bioactive peptides existing in vivo have been considered as an important class of natural medicines for the treatment of diseases. Peptidome analysis of tissues and biofluids had provided important information about the differentially expressed bioactive peptides in vivo.

Methods: Here, we analyzed the peptidome of serous ovarian cancer tissue samples and normal ovarian epithelial tissue samples by mass spectrometry and further investigated the possible bioactive peptides that were differentially expressed.

Results: We identified 634 differentially expressed peptides, 508 of these peptides were highly abundant in serous ovarian cancer tissues, a result consistent with higher protease activity in ovarian cancer patients. The difference in preferred cleavage sites between the serous ovarian cancer tissues and normal ovarian epithelium indicated the characteristic peptidome of ovarian cancer and the nature of cancer-associated protease activity. Interestingly, KEGG pathway analysis of the peptide precursors indicated that the differentially regulated pathways in ovarian cancer are highly consistent with the pathways discovered in other cancers. Besides, we found that a proportion of the differentially expressed peptides are similar to the known immune-regulatory peptides and anti-bacterial peptides. Then we further investigated the function of the two down-regulated peptides in ovarian cancer cells and found that peptide P1DS significantly inhibited the invasion and migration of OVCAR3 and SKOV3 ovarian cancer cells.

Conclusions: Our results are the first to identify the differentially expressed peptides between the serous ovarian cancer tissue and the normal ovarian epithelium. Our results indicate that bioactive peptides involved in tumorigenesis are existed in vivo.
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http://dx.doi.org/10.1016/j.biocel.2018.12.004DOI Listing
February 2019

RETRACTED: miR-149 regulates the proliferation and apoptosis of cervical cancer cells by targeting GIT1.

Biomed Pharmacother 2018 09 20;105:1106-1116. Epub 2018 Jun 20.

Department of Gyneology, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, China. Electronic address:

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Authors. The authors have found that the Western Blot result of cyclin D1 in Figure 3C cannot be repeated using the same method and requested that their paper be retracted.
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http://dx.doi.org/10.1016/j.biopha.2018.06.075DOI Listing
September 2018

Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell-specific protein 1-associated protein X-1-induced survival, migration and proliferation.

Oncol Lett 2018 Aug 4;16(2):2629-2637. Epub 2018 Jun 4.

Department of Gynaecology, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, P.R. China.

The haematopoietic cell-specific protein 1-associated protein X-1 (HAX-1), as a mitochondrial membrane protein, induces cancer progression and metastasis. The present study aimed to investigate the role of HAX-1-induced survival, migration and proliferation of human cervical squamous carcinoma cells and to elucidate its potential molecular mechanisms. The level of HAX-1 was examined by quantitative polymerase chain reaction and western blot analyses. The survival, migration and proliferation of the human cervical squamous carcinoma SiHa cell line were measured by the water-soluble tetrazolium salt (WST-1) assay, Transwell assay and H-thymidine incorporation into DNA (H-TdR) assay, respectively. The intracellular reactive oxygen species (ROS) was estimated by the fluorescence of HDCFDA, and the mitochondrial membrane potential was tested using a JC-1 probe. The expression of the HAX-1 gene was significantly increased in human cervical carcinoma tissues relative to non-cancerous cervix tissues. Overexpression of HAX-1 increased the survival, migration and proliferation ability of SiHa cells, decreased the production of ROS, and maintained the integrity of the mitochondrial membrane and morphology. The effect brought on these cells could be abrogated by the addition of wild-type tumour protein P53 (p53) or carbonyl cyanide-p-trifluoro methoxyphenylhydrazone-induced mitochondrial dysfunction. In summary, these data support the notion that HAX-1 induced the survival, migration and proliferation of human cervical squamous carcinoma cells by inhibiting its downstream regulatory factor p53 in SiHa cells.
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http://dx.doi.org/10.3892/ol.2018.8886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036589PMC
August 2018

Band Structures and Transport Properties of High-Performance Half-Heusler Thermoelectric Materials by First Principles.

Materials (Basel) 2018 May 19;11(5). Epub 2018 May 19.

State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Half-Heusler (HH) compounds, with a valence electron count of 8 or 18, have gained popularity as promising high-temperature thermoelectric (TE) materials due to their excellent electrical properties, robust mechanical capabilities, and good high-temperature thermal stability. With the help of first-principles calculations, great progress has been made in half-Heusler thermoelectric materials. In this review, we summarize some representative theoretical work on band structures and transport properties of HH compounds. We introduce how basic band-structure calculations are used to investigate the atomic disorder in n-type NiSb ( = Ti, Zr, Hf) compounds and guide the band engineering to enhance TE performance in p-type FeSb ( = V, Nb) based systems. The calculations on electrical transport properties, especially the scattering time, and lattice thermal conductivities are also demonstrated. The outlook for future research directions of first-principles calculations on HH TE materials is also discussed.
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http://dx.doi.org/10.3390/ma11050847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978224PMC
May 2018

RBBP6 promotes human cervical carcinoma malignancy via JNK signaling pathway.

Biomed Pharmacother 2018 May 22;101:399-405. Epub 2018 Mar 22.

Department of Obstetrics and Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing 210004, China. Electronic address:

Previous studies have shown that retinoblastoma binding protein 6 (RBBP6) was overexpressed in malignant tumors and was correlated with poorer prognosis in various cancers. However, its role in cervical carcinoma has not been elucidated. This study was to investigate the relationship between RBBP6 and cervical carcinoma. Cervical carcinoma cell lines SiHa and C33a were used to assess the effect of RBBP6 on cell viability, migration, and proliferation. RBBP6 mRNA and protein levels in cervical cancer tissues increased at least three times as that in the adjacent non-cancerous tissues. Overexpression of RBBP6 in SiHa and C33a cell lines resulted in increased phosphorylated c-Jun NH-terminal kinase (p-JNK) as well as increased cell viability, migration, and proliferation. Moreover, this effect was suppressed by specific JNK inhibitor SP600125. RBBP6 might potentiate cervical carcinoma cell viability, migration and proliferation through JNK signaling pathway. RBBP6 and JNK inhibitor may be beneficial as a novel preventive and therapeutic target for cervical cancer.
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http://dx.doi.org/10.1016/j.biopha.2018.02.083DOI Listing
May 2018

Computational prediction of a high ZT of n-type MgSb-based compounds with isotropic thermoelectric conduction performance.

Phys Chem Chem Phys 2018 Mar;20(11):7686-7693

State Key Laboratory of Heavy Oil Processing, Department of Materials Science and Engineering, China University of Petroleum, Beijing 102249, People's Republic of China.

N-type MgSb-based Zintl compounds are proved to be high-performance thermoelectric materials with multiple degenerate valleys and low lattice thermal conductivity. Here, we investigate the electronic band structure and the thermoelectric properties of n-type MgSb using first-principles density functional theory. A high ZT of 3.1 at 725 K is obtained when the minimum lattice thermal conductivity and the optimal carrier concentration are reached. The calculated thermoelectric performance demonstrates that MgSb possesses an isotropic character in thermoelectric transport. Furthermore, the calculated lattice thermal conductivity κ reveals that the unusually low κ in MgSb predominantly originates from the large Grüneisen parameter γ.
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http://dx.doi.org/10.1039/c7cp08680fDOI Listing
March 2018

Organ-on-Chip Recapitulates Thrombosis Induced by an anti-CD154 Monoclonal Antibody: Translational Potential of Advanced Microengineered Systems.

Clin Pharmacol Ther 2018 12 30;104(6):1240-1248. Epub 2018 Apr 30.

Janssen Pharmaceutical Research and Development, Discovery & Manufacturing Sciences, Spring House, Pennsylvania, USA.

Clinical development of Hu5c8, a monoclonal antibody against CD40L intended for treatment of autoimmune disorders, was terminated due to unexpected thrombotic complications. These life-threatening side effects were not discovered during preclinical testing due to the lack of predictive models. In the present study, we describe the development of a microengineered system lined by human endothelium perfused with human whole blood, a "Vessel-Chip." The Vessel-Chip allowed us to evaluate key parameters in thrombosis, such as endothelial activation, platelet adhesion, platelet aggregation, fibrin clot formation, and thrombin anti-thrombin complexes in the Chip-effluent in response to Hu5c8 in the presence of soluble CD40L. Importantly, the observed prothrombotic effects were not observed with Hu5c8-IgG2σ designed with an Fc domain that does not bind the FcγRIIa receptor, suggesting that this approach may have a low potential risk for thrombosis. Our results demonstrate the translational potential of Organs-on-Chips, as advanced microengineered systems to better predict human response.
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http://dx.doi.org/10.1002/cpt.1054DOI Listing
December 2018

Multidrug resistant lncRNA profile in chemotherapeutic sensitive and resistant ovarian cancer cells.

J Cell Physiol 2018 06 2;233(6):5034-5043. Epub 2018 Jan 2.

Department of Obstetrics and Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

Most ovarian cancer patients are chemosensitive initially, but finally relapse with acquired chemoresistance. Multidrug-resistance is the extremely terrible situation. The mechanism for the acquired chemoresistance of ovarian cancer patients is still not clear. LncRNAs have been recognized as the important regulator of a variety of biological processes, including the multidrug-resistant process. Here, we carried out the lncRNA sequencing of the ovarian cancer cell line A2780 and the paxitaxel resistant cell line A2780/PTX which is also cross resistant to the cisplatin and epirubicin. Through integrating the published data with the cisplatin resistant lncRNAs in ovarian cancer cell line or ovarian cancer patients, 5 up-regulated and 21 down-regulated lncRNAs are considered as the multidrug-resistant lncRNAs. By real-time PCR analysis, we confirmed the 5 up-regulated and 4 down-regulated multidrug resistant lncRNAs were similarly changed in both the multidrug resistant ovarian cancer cell lines and the multidrug resistant colon cancer cell lines. Furthermore, we conducted the lncRNA-mRNA co-expression network to predict the potential multidrug resistant lncRNAs' targets. Interestingly, the multidrug resistant genes ABCB1, ABCB4, ABCC3, and ABCG2 are all co-expressed with lncRNA CTD-2589M5.4. Our results provide the valuable information for the understanding of the lncRNA function in the multidrug resistant process.
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http://dx.doi.org/10.1002/jcp.26369DOI Listing
June 2018

Adenosine A receptor mediates hypnotic effects of ethanol in mice.

Sci Rep 2017 10 4;7(1):12678. Epub 2017 Oct 4.

Department of Pharmacology and Shanghai Key Laboratory of Bioactive Small Molecules, School of Basic Medical Sciences, State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 200032, China.

Ethanol has extensive effects on sleep and daytime alertness, causing premature disability and death. Adenosine, as a potent sleep-promoting substance, is involved in many cellular and behavioral responses to ethanol. However, the mechanisms of hypnotic effects of ethanol remain unclear. In this study, we investigated the role of adenosine in ethanol-induced sleep using C57BL/6Slac mice, adenosine A receptor (AR) knockout mice, and their wild-type littermates. The results showed that intraperitoneal injection of ethanol (3.0 g/kg) at 21:00 decreased the latency to non-rapid eye movement (NREM) sleep and increased the duration of NREM sleep for 5 h. Ethanol dose-dependently increased NREM sleep, which was consistent with decreases in wakefulness in C57BL/6Slac mice compared with their own control. Caffeine (5, 10, or 15 mg/kg), a nonspecific adenosine receptor antagonist, dose-dependently and at high doses completely blocked ethanol-induced NREM sleep when administered 30 min prior to (but not after) ethanol injection. Moreover, ethanol-induced NREM sleep was completely abolished in AR knockout mice compared with wild-type mice. These findings strongly indicate that AR is a key receptor for the hypnotic effects of ethanol, and pretreatment of caffeine might be a strategy to counter the hypnotic effects of ethanol.
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http://dx.doi.org/10.1038/s41598-017-12689-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627250PMC
October 2017

The common promoter polymorphism rs11666254 downregulates FPR2/ALX expression and increases risk of sepsis in patients with severe trauma.

Crit Care 2017 Jul 6;21(1):171. Epub 2017 Jul 6.

Department of Blood Transfusion, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping District, Chongqing, 400042, China.

Background: Formyl peptide receptor 2-lipoxin receptor (FPR2/ALX) modulates the anti-inflammatory response and therefore may be a target for treating sepsis. The purpose of this study was to investigate the association between genetic variants of the FPR2/ALX gene and sepsis after severe trauma as well as to further analyze the functions of sepsis-related genetic polymorphisms.

Methods: Three tag single-nucleotide polymorphisms (tag SNPs) that captured all common alleles across the FPR2/ALX genomic region were genotyped using pyrosequencing in an initial sample consisting of 275 patients with severe trauma. The rs11666254 polymorphism, which had statistical significance, was genotyped in an additional 371 patients, and logistic regression analysis was performed to determine associations between the FPR2/ALX gene polymorphism and sepsis susceptibility after severe trauma. The messenger RNA (mRNA) and protein levels of FPR2/ALX in the lipopolysaccharide-stimulated white blood cells of trauma patients were determined by performing quantitative polymerase chain reactions and Western blot analysis. Tumor necrosis factor (TNF)-α production was measured by enzyme-linked immunosorbent assay. The effects of the promoter polymorphism rs11666254 on the transcription activity of FPR2/ALX were analyzed using a luciferase reporter assay.

Results: Among the three tag SNPs, only the rs11666254 polymorphism was found to be significantly associated with sepsis in trauma patients, and this association persisted after a pooled analysis of all 646 trauma patients, which showed that patients who carried the A allele of rs11666254 had a significantly higher risk of developing sepsis than individuals who carried the G allele. This SNP was also significantly associated with lower FPR2/ALX mRNA and protein expression as well as higher TNF-α production from the peripheral blood leukocyte response to bacterial lipoprotein stimulation. In addition, the rs11666254 polymorphism could significantly decrease the promoter activity of the FPR2/ALX gene.

Conclusions: The rs11666254 polymorphism in the FPR2/ALX gene is a functional SNP that increases sepsis susceptibility in patients after traumatic injury.
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http://dx.doi.org/10.1186/s13054-017-1757-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499024PMC
July 2017

Thermoelectric Performance of Se/Cd Codoped SnTe via Microwave Solvothermal Method.

ACS Appl Mater Interfaces 2017 Jul 29;9(27):22612-22619. Epub 2017 Jun 29.

Centre for Future Materials, University of Southern Queensland , Springfield, Queensland 4300, Australia.

Extensive attention has been focused on thermoelectric performance optimization of SnTe because of its potential in waste heat recovery. Here, we fabricate high thermoelectric performance Se/Cd codoped SnTe octahedral particles by microwave-stimulated solvothermal method. The SnTe-based octahedral particles have sizes ranging from several micrometers to hundreds of nanometers, forming dense bulks after spark plasma sintering. Combined with the strong point defect scattering by Se and Cd dopants, a low thermal conductivity of 1.8 W m K at 773 K is obtained in the Se/Cd codoped SnCdTeSe sample. Se and Cd dopants can optimize the band structure of SnTe and boost the power factors, resulting in a promising peak ZT of ∼0.78 at 773 K in the Se/Cd codoped SnCdTeSe sample, which is significantly higher than those of undoped SnTe and Se-doped SnTe samples.
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http://dx.doi.org/10.1021/acsami.7b06083DOI Listing
July 2017

Computational prediction of high thermoelectric performance in p-type half-Heusler compounds with low band effective mass.

Phys Chem Chem Phys 2017 Feb;19(6):4411-4417

School of Space Science and Physics, Shandong University, Weihai 264209, People's Republic of China.

Half-Heusler (HH) compounds are important high temperature thermoelectric (TE) materials and have gained ever-increasing popularity. In recent years, p-type FeNbSb-based heavy-band HH compounds have attracted considerable attention with the record-high zT value of 1.5. Here, we use first-principles based methods to predict a very high zT value of 1.54 at 1200 K in p-type RuTaSb alloys. The high band degeneracy and low band effective mass contribute to a high power factor. Although the electrical thermal conductivity is high due to the high carrier mobility and hence electrical conductivity, the total thermal conductivity is moderate because of the low lattice thermal conductivity. The predicted high zT demonstrates that the p-type RuTaSb HH alloys are promising as TE materials for high temperature power generation.
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http://dx.doi.org/10.1039/c6cp07897dDOI Listing
February 2017

Early Goal-Directed Therapy in Severe Sepsis and Septic Shock: A Meta-Analysis and Trial Sequential Analysis of Randomized Controlled Trials.

J Intensive Care Med 2018 May 22;33(5):296-309. Epub 2016 Oct 22.

1 Department of Blood Transfusion, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.

Introduction: The Surviving Sepsis Campaign guidelines recommend early goal-directed therapy (EGDT) for the resuscitation of patients with sepsis; however, the recent evidences quickly evolve and convey conflicting results. We performed a meta-analysis to evaluate the effect of EGDT on mortality in adults with severe sepsis and septic shock.

Methods: We searched electronic databases to identify randomized controlled trials that compared EGDT with usual care or lactate-guided therapy in adults with severe sepsis and septic shock. Predefined primary outcome was all-cause mortality at final follow-up.

Results: We included 13 trials enrolling 5268 patients. Compared with usual care, EGDT was associated with decreased mortality (risk ratio [RR]: 0.87, 95% CI: 0.77-0.98; 4664 patients, 8 trials; Grading of Recommendations Assessment, Development, and Evaluation [GRADE] quality of evidence was moderate). Compared with lactate clearance-guided therapy, EGDT was associated with increased mortality (RR: 1.60, 95% CI: 1.24-2.06; 604 patients, 5 trials; GRADE quality of evidence was low). Patients assigned to EGDT received more intravenous fluid, red cell transfusion, vasopressor infusion, and dobutamine use within the first 6 hours than those assigned to usual care (all P values < .00001).

Conclusion: Adults with severe sepsis and septic shock who received EGDT had a lower mortality than those given usual care, the benefit may mainly be attributed to treatments administered within the first 6 hours. However, the underlying mechanisms by which lactate clearance-guided therapy benefits these patients are yet to be investigated.
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http://dx.doi.org/10.1177/0885066616671710DOI Listing
May 2018
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