Publications by authors named "Fang Liu"

4,574 Publications

  • Page 1 of 1

LncRNA LIFR-AS1 overexpression suppressed the progression of serous ovarian carcinoma.

J Clin Lab Anal 2022 Jul 2:e25470. Epub 2022 Jul 2.

Department of Gynecology, The Fourth People's Hospital of Tongxiang, Jiaxing, China.

Background: Serous ovarian carcinoma (SOC) is a common malignant tumor in female reproductive system. Long noncoding RNA (lncRNA) LIFR-AS1 is a tumor suppressor gene in colorectal cancer, but its effect and underlying mechanism in SOC are still unclear. Therefore, this study focuses on unveiling the regulatory mechanism of LIFR-AS1 in SOC.

Methods: The relationship between LIFR-AS1 expression and prognosis of SOC patients was analyzed by TCGA database and Starbase, and then, the LIFR-AS1 expression in SOC tissues and cells was detected by quantitative real-time PCR (qRT-PCR) and in situ hybridization (ISH). Besides, the relationship between LIFR-AS1 and clinical characteristics was analyzed. Also, the effects of LIFR-AS1 on the biological behaviors of SOC cells were measured by Cell Counting Kit-8, colony formation, and wound-healing and Transwell assays, respectively. Western blot and qRT-PCR were employed to determine the protein expressions of genes related to proliferation (PCNA), apoptosis (cleaved caspase-3), epithelial-mesenchymal transition (E-cadherin, N-cadherin, and Snail).

Results: LIFR-AS1 was lowly expressed in SOC, which was correlated with the poor prognosis of SOC patients. Low expression of LIFR-AS1 in SOC was associated with the tumor size, clinical stage, lymph node metastasis, and distant metastasis. LIFR-AS1 overexpression promoted the expressions of cleaved caspase-3 and E-cadherin while suppressing the malignant behaviors (proliferation, migration, and invasion) of SOC cells, the expressions of PCNA, N-cadherin, and Snail. Besides, silencing LIFR-AS1 exerted the effects opposite to overexpressed LIFR-AS1.

Conclusion: LIFR-AS1 overexpression inhibits biological behaviors of SOC cells, which may be a new therapeutic method.
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http://dx.doi.org/10.1002/jcla.24570DOI Listing
July 2022

-3-Oxo-Octanoyl Homoserine Lactone Primes Plant Resistance Against Necrotrophic Pathogen by Coordinating Jasmonic Acid and Auxin-Signaling Pathways.

Front Plant Sci 2022 14;13:886268. Epub 2022 Jun 14.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Many Gram-negative bacteria use small signal molecules, such as -acyl-homoserine lactones (AHLs), to communicate with each other and coordinate their collective behaviors. Recently, increasing evidence has demonstrated that long-chained quorum-sensing signals play roles in priming defense responses in plants. Our previous work indicated that a short-chained signal, -3-oxo-octanoyl homoserine lactone (3OC8-HSL), enhanced Arabidopsis resistance to the hemi-biotrophic bacteria pv. DC3000 through priming the salicylic acid (SA) pathway. Here, we found that 3OC8-HSL could also prime resistance to the necrotrophic bacterium ssp. () through the jasmonic acid (JA) pathway, and is dependent on auxin responses, in both Chinese cabbage and Arabidopsis. The subsequent invasion triggered JA accumulation and increased the down-stream genes' expressions of JA synthesis genes (, , and ) and JA response genes ( and ). The primed state was not observed in the Arabidopsis - and mutants, which indicated that the primed resistance to was dependent on the JA pathway. The 3OC8-HSL was not transmitted from roots to leaves and it induced indoleacetic acid (IAA) accumulation and the and auxin-responsive genes' expressions in seedlings. When Arabidopsis and Chinese cabbage roots were pretreated with exogenous IAA (10 μM), the plants had activated the JA pathway and enhanced resistance to , which implied that the JA pathway was involved in AHL priming by coordinating with the auxin pathway. Our findings provide a new strategy for the prevention and control of soft rot in Chinese cabbage and provide theoretical support for the use of the quorum-sensing AHL signal molecule as a new elicitor.
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http://dx.doi.org/10.3389/fpls.2022.886268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237615PMC
June 2022

Exploration of the Molecular Mechanism of Danzhi Xiaoyao Powder in Endometrial Cancer through Network Pharmacology.

Evid Based Complement Alternat Med 2022 13;2022:8330926. Epub 2022 Jun 13.

Department of Obstetrics and Gynecology, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, China.

Endometrial cancer (EC) is a common malignant tumor of the female reproductive system. Current treatments such as surgery and long-term hormone therapy are ineffective and have side effects. Danzhi Xiaoyao powder (DXP) can inhibit the growth of EC cells and induce apoptosis, but the pharmacological and molecular mechanisms of anticancer effects are still unclear. In this study, active components and potential targets of DXP were obtained from public databases. Protein effects and regulatory pathways of common targets were analyzed by protein-protein interaction (PPI), GO and KEGG. The results of network pharmacology showed that there are 87 common targets between EC and DXP. GO enrichment analysis showed that these targets were associated with response to oxidative stress, response to nutrient levels, hormone receptor binding and nuclear hormone receptor binding, etc. The results of KEGG analysis indicated that IL-17, TNF, PI3K/AKT, and RAS/RAF/MEK/ERK (ERK) signaling pathway were enriched in the anti-EC of DXP. Additionally, we cultured HEC-1B and KLE cells for validate experiments. DXP showed an inhibition of proliferation, migration, and cell cycle of both cells. Moreover, the expression of RAS, p-RAF, p-MEK, ERK, and p-ERK related proteins were downregulated. In conclusion, DXP might inhibit the proliferation of EC cells via apoptosis. Furthermore, DXP-induced inhibition of EC development might involve RAS/RAF/MEK/ERK pathway.
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http://dx.doi.org/10.1155/2022/8330926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239783PMC
June 2022

Government Environmental Governance and Enterprise Coordinated Green Development under the Goal of "Double Carbon".

J Environ Public Health 2022 21;2022:6605935. Epub 2022 Jun 21.

Law School & Intellectual Property School, Jinan University, Guangzhou 510632, China.

China's 13th five-year plan puts forward the development concept of "green development" and takes the construction of green financial system as the development strategy, which shows that the concept of environmental governance and green development is becoming more and more important for national economic development. It is a major strategic task for China to build an environmental governance system dominated by the government and dominated by enterprises and implement the main responsibility of enterprise environmental governance. At present, the environmental supervision of local governments needs to be improved urgently. It is necessary to strengthen the production regulation of enterprises and promote the emission of "three wastes" up to the standard based on the principle of "polluter pays." The green development of enterprises is not only conducive to the improvement of their own "three effects" and corporate image but also has the external spillover effect of giants and is conducive to the construction of ecological civilization. Therefore, this study will focus on the collaborative governance in the field of environment, explore the ideal collaborative governance framework in the process of environmental collaborative governance, as well as the difficulties and optimization paths of collaboration, and put forward corresponding suggestions for the current environmental governance.
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http://dx.doi.org/10.1155/2022/6605935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239759PMC
June 2022

The Acute Toxicities and Efficacy of Concurrent Chemotherapy With Docetaxel Plus Cisplatin, or Docetaxel, or Cisplatin and Helical Tomotherapy in Patients With Locoregionally Advanced Nasopharyngeal Carcinoma: A Randomized Single-Center Phase II Trial.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221109974

Department of Radiation Oncology, 104607First Medical Center of Chinese PLA General Hospital, Beijing, China.

The objective of this trial is to evaluate and compare the acute toxicity in patients with locoregionally advanced nasopharyngeal carcinoma (LA-NPC) treated with docetaxel plus cisplatin, or docetaxel, or cisplatin concurrently with helical tomotherapy during concurrent chemoradiotherapy (CCRT). In a prospective, single-center, open-label, randomized phase II study, after 2 cycles of induction chemotherapy with docetaxel plus cisplatin regimen, 125 patients with LA-NPC (stage III and IVA, UICC eighth) diagnosed pathologically from June 2017 to November 2019 were randomized into CCRT with docetaxel plus cisplatin group (25 patients), CCRT with docetaxel group (50 patients), and CCRT with cisplatin group (50 patients). The incidence of grade 3 or 4 acute toxicities and clinical efficacy were analyzed among the 3 groups. Safety evaluation was completed in all the 125 patients, during the CCRT period, 66.4% of patients completed 3 cycles of chemotherapy, 24.0% completed 2 cycles of chemotherapy, and 9.6% completed 1 cycle of chemotherapy according to the research plan. The incidence of grade 3 or 4 acute toxicity in CCRT with docetaxel plus cisplatin (DP), docetaxel (D), and cisplatin (P) groups was 88.0%, 72.0%, and 56.0%, respectively. The incidence of grade 3 or 4 acute toxicities in the DP group was significantly higher than that in the D and P groups ( = .015), no significant difference was detected between the D and P groups ( = .096). The most common toxicities were mucositis (40.0%), leukopenia (29.6%), neutropenia (26.4%), and pharyngo-esophagitis (12.0%); compared to D and P groups, DP group did not significantly improved the 3-year overall survival (96.0% vs 87.0% and 87.6%), progression-free survival (92.0% vs 79.7% and,76.9%), locoregional failure-free survival (96.0% vs 91.8% and 92.7%), and distant failure-free survival (100% vs 90.0% and 89.0%), there were no significant difference in survival data among the 3 groups (all > .05). Higher survival benefits did not achieve from intensified CCRT with DP, CCRT with P or D obtained similar short-term survival outcomes with similar acceptable toxicities in LA-NPC patients.
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http://dx.doi.org/10.1177/15330338221109974DOI Listing
July 2022

The Invasive Species Houtt. as a Promising Natural Agent for Cardiovascular and Digestive System Illness.

Front Pharmacol 2022 13;13:863707. Epub 2022 Jun 13.

State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Polygoni Cuspidati Rhizoma et Radix, the dry roots and stems of Houtt (called Huzhang, HZ in Chinese), is a traditional and popular chinese medicinal herb for thousands of years. As a widely used ethnomedicine in Asia including China, Japan, and Korea, HZ can invigorate the blood, cool heat, and resolve toxicity, which is commonly used in the treatment of favus, jaundice, scald, and constipation. However, HZ is now considered an invasive plant in the United States and many European countries. Therefore, in order to take advantage of HZ and solve the problem of biological invasion, scholars around the world have carried out abundant research studies on HZ. Until now, about 110 compounds have been isolated and identified from HZ, in which anthraquinones, stilbenes, and flavonoids would be the main bioactive ingredients for its pharmacological properties, such as microcirculation improvement, myocardial protective effects, endocrine regulation, anti-atherosclerotic activity, anti-oxidant activity, anti-tumor activity, anti-viral activity, and treatment of skin inflammation, burns, and scalds. HZ has a variety of active ingredients and broad pharmacological activities. It is widely used in health products, cosmetics, and even animal husbandry feed and has no obvious toxicity. Efforts should be made to develop more products such as effective drugs, health care products, cosmetics, and agricultural and animal husbandry products to benefit mankind.
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http://dx.doi.org/10.3389/fphar.2022.863707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234309PMC
June 2022

Plastid RNA editing reduction accompanied with genetic variations in , a genus with diverse lifestyle modes.

Plant Divers 2022 May 12;44(3):316-321. Epub 2021 Jul 12.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.

Recent sequencing efforts have broadly uncovered the evolutionary trajectory of plastid genomes (plastomes) of flowering plants in diverse habitats, yet our knowledge of the evolution of plastid posttranscriptional modifications is limited. In this study, we generated 11 complete plastomes and performed ultra-deep transcriptome sequencing to investigate the co-evolution of plastid RNA editing and genetic variation in , a genus with diverse trophic lifestyles. Genome size and gene content is reduced in terrestrial and green mycoheterotrophic orchids relative to their epiphytic relatives. This could be partly due to extensive losses and pseudogenization of genes for the plastid NADH dehydrogenase-like complex, but independent pseudogenization of genes has also occurred in the epiphyte , which was reported to use strong crassulacean acid metabolism photosynthesis. RNA editing sites are abundant but variable in number among plastomes. The nearly twofold variation in editing abundance is mainly due to extensive reduction of ancestral editing sites in transcripts of terrestrial, mycoheterotrophic, and plastomes. The co-occurrence of editing reduction and pseudogenization in genes suggests functional constraints on editing machinery may be relaxed, leading to nonrandom loss of ancestral edited sites via reduced editing efficiency. This study represents the first systematic examination of RNA editing evolution linked to plastid genome variation in a single genus. We also propose an explanation for how genomic and posttranscriptional variations might be affected by lifestyle-associated ecological adaptation strategies in .
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http://dx.doi.org/10.1016/j.pld.2021.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209865PMC
May 2022

R-spondin 3 inhibits high glucose-induced endothelial activation through LGR4/Wnt/β-catenin pathway.

J Cardiovasc Pharmacol 2022 May 10. Epub 2022 May 10.

Institute for Developmental and Regenerative Cardiovascular Medicine, Xinhua, Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China.

Abstract: High glucose-induced endothelial activation plays critical roles in the development of diabetic vascular complications. R-spondin 3 could inhibit inflammatory damage, and diabetic vascular inflammation is secondary to endothelial activation. Here we identify R-spondin 3 as a novel regulator of high glucose-induced endothelial activation. We found that the serum levels of R-spondin 3 were significantly reduced in type 2 diabetic patients and db/db mice. We observed that the increased expressions of vascular cell adhesion molecule (VCAM-1), intercellular cell adhesion molecule (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) (endothelial activation makers) in high glucose-stimulated HUVECs could be inhibited by overexpressing R-spondin 3 or human R-spondin 3 recombinant protein. Subsequently, high glucose-induced adhesion and migration of THP-1 cells to HUVECs were markedly suppressed by the overexpression of R-spondin 3 in HUVECs. Moreover, the inhibitory effect of R-spondin 3 on the expressions of VCAM-1, ICAM-1 and MCP-1 in high glucose-treated HUVECs could be blocked by knockdown of leucine-rich G protein coupled receptor 4 (LGR4, R-spondin 3 receptor) or the specific inhibitor of Wnt/β-catenin pathway. Taken together, R-spondin 3 could suppress high glucose-induced endothelial activation through LGR4/Wnt/β-catenin pathway.
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http://dx.doi.org/10.1097/FJC.0000000000001295DOI Listing
May 2022

Self-Supervised Self-Organizing Clustering Network: A Novel Unsupervised Representation Learning Method.

IEEE Trans Neural Netw Learn Syst 2022 Jun 29;PP. Epub 2022 Jun 29.

Deep learning-based clustering methods usually regard feature extraction and feature clustering as two independent steps. In this way, the features of all images need to be extracted before feature clustering, which consumes a lot of calculation. Inspired by the self-organizing map network, a self-supervised self-organizing clustering network (S OCNet) is proposed to jointly learn feature extraction and feature clustering, thus realizing a single-stage clustering method. In order to achieve joint learning, we propose a self-organizing clustering header (SOCH), which takes the weight of the self-organizing layer as the cluster centers, and the output of the self-organizing layer as the similarities between the feature and the cluster centers. In order to optimize our network, we first convert the similarities into probabilities which represents a soft cluster assignment, and then we obtain a target for self-supervised learning by transforming the soft cluster assignment into a hard cluster assignment, and finally we jointly optimize backbone and SOCH. By setting different feature dimensions, a Multilayer SOCHs strategy is further proposed by cascading SOCHs. This strategy achieves clustering features in multiple clustering spaces. S OCNet is evaluated on widely used image classification benchmarks such as Canadian Institute For Advanced Research (CIFAR)-10, CIFAR-100, Self-Taught Learning (STL)-10, and Tiny ImageNet. Experimental results show that our method significant improvement over other related methods. The visualization of features and images shows that our method can achieve good clustering results.
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http://dx.doi.org/10.1109/TNNLS.2022.3185638DOI Listing
June 2022

Cold-Starting All-Solid-State Batteries from Room Temperature by Thermally Modulated Current Collector in Sub-Minute.

Adv Mater 2022 Jun 27:e2202848. Epub 2022 Jun 27.

Department of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.

All-solid-state batteries (ASSBs) show great potential as high-energy and high-power energy storage devices but their attainable energy/power density at room temperature is severely reduced because of the sluggish kinetics of lithium-ion transport. Here we first reported a thermally modulated current collector (TMCC), which can rapidly cold-start ASSBs from room temperature to operating temperatures (70∼90 °C) in less than one minute, and simultaneously enhance the transient peak power density by 15-fold compared to one without heating. This TMCC is prepared by integrating a uniform, ultrathin (∼200 nm) nickel layer as a thermal modulator within an ultralight polymer-based current collector. By isolating the thermal modulator from the ion/electron pathway of ASSBs, it can provide fast, stable heat control yet does not interfere with regular battery operation. Moreover, this ultrathin (13.2 μm) TMCC effectively shortens the heat transfer pathway, minimizes heat losses, and mitigates the formation of local hot spots. The simulated heating energy consumption can be as low as ∼3.94% of total battery energy. This TMCC design with good tunability opens new frontiers towards smart energy storage devices in the future from the current collector perspective. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202202848DOI Listing
June 2022

PD-L2 Serves as a Potential Prognostic Biomarker That Correlates With Immune Infiltration and May Predict Therapeutic Sensitivity in Lower-Grade Gliomas.

Front Oncol 2022 8;12:860640. Epub 2022 Jun 8.

Department of Neurosurgery, The affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, China.

Although patients with lower-grade gliomas (LGGs; grades II and III) have a relatively favorable prognosis, patients frequently relapse and tend to progress to higher-grade gliomas, leading to treatment resistance, poor survival, and ultimately treatment failure. However, until now, thorough research has not yet been reported on the relationship between PD-L2 and immune infiltration and therapeutic sensitivity to immunotherapy and TMZ-based chemotherapy of LGGs. In this study, we found that the expression of PD-L2 is upregulated in glioma, with high PD-L2 expression predicting a worse prognosis. Univariate and multivariate Cox regression analysis both indicated that PD-L2 represented an independent prognostic factor with high accuracy in survival prediction for LGGs. A nomogram comprising of age, grade, IDH mutation, and PD-L2 was established for predicting OS. Additionally, PD-L2 was found to be remarkably correlated with immune infiltration and some anti-tumor immune functions. The degree of PD-L2 expression was also found to be strongly related to the prediction of therapeutic sensitivity to immunotherapy and TMZ-based chemotherapy. Furthermore, immunohistochemistry demonstrated that PD-L2 and the macrophage biomarker CD68 were both increased in glioma, with PD-L2 expression having a strong positive connection with CD68 expression. Taken together, PD-L2 is a prognostic biomarker for LGGs patients that may provide novel insights into glioma individualized therapeutic strategies and guide effective immunotherapy and chemotherapy.
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http://dx.doi.org/10.3389/fonc.2022.860640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213741PMC
June 2022

Correction to "Spatiotemporally dynamic therapy with shape-adaptive drug-gel for the improvement of tissue regeneration with ordered structure" [Bioact. Mater. 8 (2022) 165-176].

Bioact Mater 2023 Feb 9;20:318. Epub 2022 Jun 9.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.

[This corrects the article DOI: 10.1016/j.bioactmat.2021.06.015.].
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http://dx.doi.org/10.1016/j.bioactmat.2022.04.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192695PMC
February 2023

Genome-Wide Identification and Characterization of Genes in Reveals Their Potential Role in Trichome Development.

Front Genet 2022 8;13:921096. Epub 2022 Jun 8.

State Key Laboratory of Cotton Biology /Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, China.

Trichomes protect plants against insects, microbes, herbivores, and abiotic damages and assist seed dispersal. The function of genes have been found to be involved in the trichome development but the research on the underlying genetic and molecular mechanisms are extremely limited. Herein, genome wide identification and characterization of genes was performed. In total, 26 family members were identified in Gossypium species. Phylogenetic analysis, structural characteristics, and synteny analysis of s showed the conserved evolution relationships of . The promoter analysis of genes revealed hormone, stress, and development-related cis-elements. Gene ontology (GO) enrichment analysis showed that the genes were largely related to biological regulation, developmental process, multicellular organismal process. Protein-protein interaction analysis predicted several trichome development related proteins (SIM, LGO, and GRL) directly interacting with genes. Further, nine putative -miRNAs were also identified, targeting genes. RNA-Seq data of (with trichomes) and (with no trichomes) was used to perform the co-expression network analysis. was identified as a hub gene in a co-expression network analysis. RT-qPCR of gene in different tissues suggests that this gene has higher expressions in the petiole and might be a key candidate involved in the trichome development. Virus induced gene silencing of confirms its role in trichome development and elongation. Current results provide proofs of the possible role of genes and provide preliminary information for further studies of functions in trichome development.
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http://dx.doi.org/10.3389/fgene.2022.921096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213653PMC
June 2022

In Situ Atomic-Scale Observation of Electrochemical (De)potassiation in Te Nanowires.

Small 2022 Jun 24:e2200844. Epub 2022 Jun 24.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, 430070, P. R. China.

Potassium-ion batteries (PIBs) have great potential in energy storage due to their high abundance and low cost of potassium resources. Tellurium (Te) is a promising PIB cathode due to its high volumetric capacity and good electronic conductivity. However, the electrochemical (de)potassiation mechanism of Te remains elusive due to the lack of an effective method of directly observing the dynamic reaction at atomic resolution. Here, the phase transformations of single crystal Te on (de)potassiation are clearly revealed by in situ high-resolution transmission electron microscopy and electron diffraction. Te undergoes a consecutive phase transformation during potassiation: from Te to K Te in the initial potassiation, and then part of the K Te to K Te on further potassiation. The reaction has extremely high reversibility in the following depotassiation. By atomic-scale observation, an anisotropic reaction mechanism where K intercalates into Te crystalline lattice preferentially through the (001) plane (having a large d-spacing) is established during potassiation. While in the depotassiation process, K ions extract from the polycrystalline K Te along the same diffusion path to form single crystal Te, indicating the potassium storage is highly reversible. The strong orientation-dependent (de)potassiation mechanism revealed by this work provides implications for the future design of nanostructured cathodes for high-performance PIBs.
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http://dx.doi.org/10.1002/smll.202200844DOI Listing
June 2022

Ferulic Acid Prevents Nonalcoholic Fatty Liver Disease by Promoting Fatty Acid Oxidation and Energy Expenditure in C57BL/6 Mice Fed a High-Fat Diet.

Nutrients 2022 Jun 18;14(12). Epub 2022 Jun 18.

College of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang 712100, China.

There is a consensus that ferulic acid (FA), the most prominent phenolic acid in whole grains, displays a protective effect in non-alcoholic fatty liver disease (NAFLD), though its underlying mechanism not fully elucidated. This study aimed to investigate the protective effect of FA on high-fat diet (HFD)-induced NAFLD in mice and its potential mechanism. C57BL/6 mice were divided into the control diet (CON) group, the HFD group, and the treatment (HFD+FA) group, fed with an HFD and FA (100 mg/kg/day) by oral gavage for 12 weeks. Hematoxylin and eosin (H&E) staining and Oil Red O staining were used to evaluate liver tissue pathological changes and lipid accumulation respectively. It was demonstrated that FA supplementation prevented HFD-induced NAFLD, which was evidenced by the decreased accumulation of lipid and hepatic steatosis in the HFD+FA group. Specifically, FA supplementation decreased hepatic triacylglycerol (TG) content by 33.5% ( < 0.01). Metabolic cage studies reveal that FA-treated mice have elevated energy expenditure by 11.5% during dark phases. Mechanistically, FA treatment increases the expression of rate-limiting enzymes of fatty acid oxidation and ketone body biosynthesis CPT1A, ACOX1 and HMGCS2, which are the peroxisome proliferator-activated receptors α (PPARα) targets in liver. In conclusion, FA could effectively prevent HFD-induced NAFLD possibly by activating PPARα to increase energy expenditure and decrease the accumulation of triacylglycerol in the liver.
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http://dx.doi.org/10.3390/nu14122530DOI Listing
June 2022

Reactive Oxygen Species Distribution Involved in Stipe Gradient Elongation in the Mushroom .

Cells 2022 06 11;11(12). Epub 2022 Jun 11.

Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

The mushroom stipe raises the pileus above the substrate into a suitable position for dispersing spores. The stipe elongates at different speeds along its length, with the rate of elongation decreasing in a gradient from the top to the base. However, the molecular mechanisms underlying stipe gradient elongation are largely unknown. Here, we used the model basidiomycete mushroom to investigate the mechanism of mushroom stipe elongation and the role of reactive oxygen species (ROS) signaling in this process. Our results show that O and HO exhibit opposite gradient distributions in the stipe, with higher O levels in the elongation region (ER), and higher HO levels in the stable region (SR). Moreover, NADPH-oxidase-encoding genes are up-regulated in the ER, have a function in producing O, and positively regulate stipe elongation. Genes encoding manganese superoxide dismutase (MnSOD) are up-regulated in the SR, have a function in producing HO and negatively regulate stipe elongation. Altogether, our data demonstrate that ROS (O/HO) redistribution mediated by NADPH oxidase and MnSODs is linked to the gradient elongation of the stipe.
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http://dx.doi.org/10.3390/cells11121896DOI Listing
June 2022

Occurrence, distribution, source identification, and risk assessment of organophosphate esters in the coastal waters of Beibu Gulf, South China Sea: Impacts of riverine discharge and fishery.

J Hazard Mater 2022 08 26;436:129214. Epub 2022 May 26.

Guangxi Laboratory on the Study of Coral Reefs in the South China Sea; Coral Reef Research Center of China; School of Marine Sciences, Guangxi University, Nanning 530004, China.

As emerging pollutants, the environmental geochemistry of organophosphate esters (OPEs) in the coastal zone with multiple functional areas are still less recognized. This study investigated spatiotemporal distribution, sources and risks of 11 widely used OPEs in surface waters from seagoing rivers and multiple coastal functional areas of the Beibu Gulf. The results indicated that significantly higher ∑OPEs (total concentrations of 11 OPEs, ng/L) occurred in summer (34.2-1227) than in winter (20.6-840), as a result of the high emission caused by climate reasons. In general, higher ∑OPEs occurred in rivers (41.2-1227) than in the coast (34.2-809) in summer, especially in the urban rivers, while in winter, higher ∑OPEs occurred in the coast (23.4-840 vs 20.6-319 in rivers) because of obviously higher ∑OPEs in marine fishery areas (99-840). Source identification revealed that fishery activity, especially fishing vessels, and urban rivers were the main sources of OPEs in the Beibu Gulf. For the individual OPE, only tri-n-butyl phosphate (TNBP) may have ecological risks to aquatic organisms in a few sites, but if considering the additive effects, the OPEs mixtures would pose a high risk to algae and low to medium threats to crustaceans and fish.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129214DOI Listing
August 2022

Correction: Use of latent profile analysis and k-means clustering to identify student anxiety profiles.

BMC Psychiatry 2022 Jun 23;22(1):425. Epub 2022 Jun 23.

School of Health Management, China Medical University, No.77 Puhe Road, Shenyang North New District, Shenyang, 110122, Liaoning, China.

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http://dx.doi.org/10.1186/s12888-022-04066-zDOI Listing
June 2022

Peripherally restricted transthyretin-based delivery system for probes and therapeutics avoiding opioid-related side effects.

Nat Commun 2022 Jun 23;13(1):3590. Epub 2022 Jun 23.

Department of Pharmaceutics and Medicinal Chemistry, Thomas J. Long School of Pharmacy, University of the Pacific, Stockton, CA, 95211, US.

Several investigations into the sites of action of opioid analgesics have utilized peripherally acting mu-opioid receptor antagonists (PAMORAs), which have been incorrectly assumed to possess limited permeability across the blood-brain barrier. Unfortunately, the poor pharmacokinetic properties of current PAMORAs have resulted in misunderstandings of the role of central nervous system and gastrointestinal tract in precipitating side effects such as opioid-induced constipation. Here, we develop a drug delivery approach for restricting the passage of small molecules across the blood-brain barrier. This allows us to develop naloxone- and oxycodone-based conjugates that display superior potency, peripheral selectivity, pharmacokinetics, and efficacy in rats compared to other clinically used PAMORAs. These probes allow us to demonstrate that the mu-opioid receptors in the central nervous system have a fundamental role in precipitating opioid-induced constipation. Therefore, our conjugates have immediate use as pharmacological probes and potential therapeutic agents for treating constipation and other opioid-related side effects.
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http://dx.doi.org/10.1038/s41467-022-31342-zDOI Listing
June 2022

Mycoplasma genitalium and Chlamydia trachomatis infection among women in Southwest China: a retrospective study.

Epidemiol Infect 2022 Jun 23:1-19. Epub 2022 Jun 23.

Department of Laboratory Medicine, West China Second University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, P.R. China.

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http://dx.doi.org/10.1017/S0950268822001066DOI Listing
June 2022

Fragaria mitogenomes evolve rapidly in structure but slowly in sequence and incur frequent multinucleotide mutations mediated by micro-inversions.

New Phytol 2022 Jun 22. Epub 2022 Jun 22.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.

Plant mitochondrial DNA has been described as evolving rapidly in structure but slowly in sequence. However, many of the noncoding portions of plant mitogenomes are not homologous among species, raising questions about the rate and spectrum of mutations in noncoding regions. Recent studies suggested that the lack of homology in noncoding regions could be due to increased sequence divergence. We compared 30 kb of coding and 200 kb of noncoding DNA from 13 sequenced Fragaria mitogenomes, followed by analyzing the rate of sequence divergence, micro-inversion events and structural variations. Substitution rates in synonymous sites and nongenic sites are nearly identical, suggesting the genome-wide point mutation rate is generally consistent. A surprisingly high number of large multinucleotide substitutions were detected in Fragaria mitogenomes, which may have resulted from microinversion events and could affect phylogenetic signal and local rate estimates. Fragaria mitogenomes preferentially accumulate deletions relative to insertions, and substantial genomic arrangements, while mutation rates could positively associate with these sequence and structural changes among species. Together, these observations suggest that plant mitogenomes exhibit low point mutations genome-wide but exceptionally high structural variations, and our results favor a gain-and-loss model for the rapid loss of homology among plant mitogenomes.
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http://dx.doi.org/10.1111/nph.18334DOI Listing
June 2022

[Cumulative effects of -function in the research of point patterns].

Ying Yong Sheng Tai Xue Bao 2022 May;33(5):1275-1282

Inner Mongolia Meteorological Institute, Hohhot 010051, China.

The spatial pattern of plant population is one of primary issues in ecological research. Point pattern analy-sis is considered as an important method to study the spatial pattern of plant population. Ripley's function has been commonly used for point pattern analysis. However, the cumulative effect of Ripley's function may lead to specific spatial pattern charcteristics. To explore how the cumulative effect of Ripley's function affects population pattern, the data of clumped distribution, random distribution and regular distribution of were simulated by R software. All data generated by R software were analyzed by Ripley's function and the non-cumulative pairwise correlation function (). The results showed that for clumped distribution (or regular distribution), the cumulative effect of Ripley's function was manifested in two aspects. On the one hand, the scale of clumped distribution (or regular distribution) was increased due to Ripley's function. On the other hand, Ripley's function could detect the difference of the distribution of cluster (or negative interaction range) in the sampling space, exhibiting different pattern characteristics. For random distribution, Ripley's function had no cumulative effect. In conclusion, the combination of Ripley's function and pairwise correlation function by collecting replicate samples could better reveal the essential characteristics of the pattern in the study of population pattern.
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http://dx.doi.org/10.13287/j.1001-9332.202205.005DOI Listing
May 2022

Visible-Light-Promoted Cross-Coupling of -Aryl Oximes and Nitrostyrenes to Access Cyanoalkylated Alkenes.

Org Lett 2022 Jul 21;24(25):4640-4644. Epub 2022 Jun 21.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P.R. China.

A photoinduced, photocatalyst-free cyanoalkylation of nitrostyenes was explored, affording a series of cyanoalkylated alkenes in moderate to good yields. Mechanistic studies reveal that an electron donor-acceptor complex formed between -aryl oximes and DIPEA is presumably involved in this process. The excellent functional group compatibility of this newly designed synthetic protocol allows for cyanoalkylation of structurally varied substrates, which offers an eco-friendly pathway for the assembly of cyanoalkylated alkenes.
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http://dx.doi.org/10.1021/acs.orglett.2c01750DOI Listing
July 2022

Adsorption of acetone, ethyl acetate and toluene by beta zeolite/diatomite composites: preparation, characterization and adsorbability.

Environ Sci Pollut Res Int 2022 Jun 20. Epub 2022 Jun 20.

Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130021, China.

The hierarchical porous composites (Beta/Dt) were prepared by secondary growth method using natural diatomite and beta zeolite. Moreover, XRD, SEM, and BET characterize the composite's composition, surface structure, and pore structure. The adsorbability of Beta/Dt was evaluated by adsorption of three common volatile organic compounds (VOCs) of the printing industry: acetone, ethyl acetate, and toluene. The results show that under the optimum preparation condition, the adsorption capacities of the three VOCs on Beta/Dt were about 3.5 times those of pure beta zeolite and 4.7-35.3 times those of diatomite, respectively. It indicates the synergistic adsorption effect between beta zeolite and diatomite. The superior adsorption capacity of Beta/Dt can be attributed to the suitable micropore size, the increase of the diffusion channels, and the chemical adsorption on modification diatomite. The adsorption of acetone, ethyl acetate, and toluene on Beta/Dt conformed to the pseudo-second-order kinetic model. In contrast, adsorption isotherms conformed to the Langmuir model, meaning that both physical and chemical adsorption occurred simultaneously during the adsorption process, and the adsorption belonged to the monolayer adsorption. The chemical adsorption mechanism can be ascribed to the nucleophilic reaction between the three VOCs (acetone, ethyl acetate, and toluene) and Beta/Dt with positive charges resulting from the modification diatomite. Furthermore, the composite could still keep more than 90% of the adsorption capacity of the original adsorbent after five regeneration cycles.
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http://dx.doi.org/10.1007/s11356-022-21308-5DOI Listing
June 2022

Rapid and Ultrasensitive Detection of Methicillin-Resistant Based on CRISPR-Cas12a Combined With Recombinase-Aided Amplification.

Front Microbiol 2022 3;13:903298. Epub 2022 Jun 3.

Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, China.

is one of the main pathogens causing hospital and community-acquired infections, in particular, infections caused by methicillin-resistant (MRSA) cause a higher mortality rate than those caused by methicillin-sensitive strains, which poses a serious global public health problem. Therefore, rapid and ultrasensitive detection of patients with clinical MRSA infection and timely control of infection are essential. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) based on nucleic acid detection methods are well-known for its high specificity and sensitivity and programmability. Here, we successfully proposed a method based on CRISPR-Cas12a combined with recombinase-aided amplification (RAA) through fluorescent readout to achieve accurate identification and highly sensitive detection of MRSA in clinical samples. Results showed that the limit of detection (LoD) of the RAA-Cas12a method could reach 10 copies/μl at 60 min of reaction. Specificity tests showed that the method could distinguish MRSA from clinically common bacteria. The results of RAA-Cas12a were consistent with that of antimicrobial susceptibility tests (AST) and polymerase chain reaction (PCR) in 83 clinical samples. These results indicated that the detection method based on RAA-Cas12a has high sensitivity and specificity, and provides important value for rapid detection of MRSA.
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http://dx.doi.org/10.3389/fmicb.2022.903298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204182PMC
June 2022

Rational and practical considerations to guide a Target Product Profile for patient-centric drug product development with measurable patient outcomes - A proposed roadmap.

Eur J Pharm Biopharm 2022 Jun 16. Epub 2022 Jun 16.

Ecole de Biologie Industrielle, 49 avenue des Genottes, 95895 Cergy Cedex, France. Electronic address:

The increasing awareness of acceptability and usability of pharmaceutical drug products by the patient as a key quality requirement continues to drive need for integrating patient centric drug product design into the pharmaceutical development process. The complex matrix of multiple drug product related decisions during the early drug development process often limits patient-centric drug product (PCDP) design options in the final commercial drug product development phase. To integrate the specific needs and perspectives of patients into drug development and product design process, a rational approach integrated into the complex development matrix is required from the start and weighs product development decision options accordingly. The aim of this work was to develop a roadmap for PCDP design in a multidisciplinary approach that leads to better usability, adherence and acceptance of the drug by patients via early integration into the development matrix. The proposed rational approach is based upon regulatory requirements and lessons learned from pediatric and geriatric drug development.
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http://dx.doi.org/10.1016/j.ejpb.2022.06.006DOI Listing
June 2022

Expert consensus on personalized initiation of glucose-lowering therapy in adults with newly diagnosed type 2 diabetes without clinical cardiovascular disease or chronic kidney disease.

J Evid Based Med 2022 Jun 18. Epub 2022 Jun 18.

Division of Endocrinology and Metabolism, Center for Diabetes and Metabolism Research, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

Since it is difficult for clinicians to make a decision among the various types of antidiabetic medications due to their great discrepancy in mechanisms, pharmacological properties, and cardiovascular/renal protection, a relatively "precision" or personalized hypoglycemic treatment suggestion is practical for type 2 diabetes (T2D) management in adults. This expert consensus makes some recommendations based on the characteristics of adult T2D patients without clinical cardiovascular disease (CVD) or chronic kidney disease (CKD) by evidence from large-scale clinical trials. The main consideration for initiating antidiabetic medications is the safety and benefits for prevention of target organ damage, such as CVD and CKD. The choice of personalized glucose-lowering therapy regarding target organ protection is based on the various effects of antidiabetic medications, patients' clinical characteristics and their key risks, as well as the sociological factors. According to the effects on glucose reduction, cardiovascular protection, renal benefit, body weight change, hypoglycemic risk, and liver function impact, the antidiabetic medications are recategorized in this consensus. Combined with the glucose control target and the different effects of hypoglycemic agents, a significant body of recommendations have been developed for optimal T2D management according to the risk factors for atherosclerotic CVD, heart failure, CKD, primary fatty liver, and hypoglycemia. This consensus gives detailed guidance on personalized antidiabetic therapy initiation in newly diagnosed T2D adults, which attaches great importance to both glucose control and target organ protection.
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http://dx.doi.org/10.1111/jebm.12474DOI Listing
June 2022

Cre/ regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration.

Elife 2022 Jun 17;11. Epub 2022 Jun 17.

Department of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.

The ability to regulate gene activity spatially and temporally is essential to investigate cell type-specific gene function during development and in postembryonic processes and disease models. The Cre/ system has been widely used for performing cell and tissue-specific conditional analysis of gene function in zebrafish. However, simple and efficient methods for isolation of stable, Cre/ regulated zebrafish alleles are lacking. Here we applied our GeneWeld CRISPR-Cas9 targeted integration strategy to generate floxed alleles that provide robust conditional inactivation and rescue. A universal targeting vector, UFlip, with sites for cloning short homology arms flanking a floxed 2A-mRFP gene trap, was integrated into an intron in and . and integration alleles resulted in strong mRFP expression, >99% reduction of endogenous gene expression, and recapitulated known indel loss of function phenotypes. Introduction of Cre led to stable inversion of the floxed cassette, loss of mRFP expression, and phenotypic rescue. and integration alleles did not cause phenotypes in combination with a loss of function mutation. Addition of Cre led to conditional inactivation by stable inversion of the cassette, gene trapping and mRFP expression, and the expected mutant phenotype. Neural progenitor Cre drivers were used for conditional inactivation and phenotypic rescue to showcase how this approach can be used in specific cell populations. Together these results validate a simplified approach for efficient isolation of Cre/ responsive conditional alleles in zebrafish. Our strategy provides a new toolkit for generating genetic mosaics and represents a significant advance in zebrafish genetics.
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http://dx.doi.org/10.7554/eLife.71478DOI Listing
June 2022

The comprehensive and systematic identification of BLCA-specific SF-regulated, survival-related AS events.

Gene 2022 Jun 13;835:146657. Epub 2022 Jun 13.

Department of Computer Science, City University of Hong Kong, Hong Kong, China. Electronic address:

Bladder urothelial carcinoma (BLCA) is a complex disease with high morbidity and mortality. Changes in alternative splicing (AS) and splicing factor (SF) can affect gene expression, thus playing an essential role in tumorigenesis. This study downloaded 412 patients' clinical information and 433 samples of transcriptome profiling data from TCGA. And we collected 48 AS signatures from SpliceSeq. LASSO and Cox analyses were used for identifying survival-related AS events in BLCA. Finally, 1,645 OS-related AS events in 1,129 genes were validated by Kaplan-Meier (KM) survival analysis, ROC analysis, risk curve analysis, and independent prognostic analysis. Finally, our survey provides an AS-SF regulation network consisting of five SFs and 46 AS events. In the end, we profiled genes that AS occurred in pan-cancer and five SFs' expression in tumor and normal samples in BLCA. We selected CLIP-seq data for validation the interaction regulated by RBP. Our study paves the way for potential therapeutic targets of BLCA.
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http://dx.doi.org/10.1016/j.gene.2022.146657DOI Listing
June 2022

Identification of MMACHC and ZEB2 mutations causing coexistent cobalamin C disease and Mowat-Wilson syndrome in a 2-year-old girl.

Clin Chim Acta 2022 Jun 13;533:31-39. Epub 2022 Jun 13.

Department of Pediatrics, the 980th Hospital of the People's Liberation Army Joint Service Support Force (Bethune International Peace Hospital), Shijiazhuang 050082, Hebei, China.

Cobalamin C (cblC) disease and Mowat-Wilson syndrome (MWS) are rare hereditary diseases. To date, there have been no reports of people suffering from these two genetic diseases, or whether there is any correlation between the two diseases. We reported a 2-year-old girl with both cblC disease and MWS. The patient initially manifested as slow weight gain, hypotonia, broad nasal bridge, high forehead, high palate arch, ear crease, patent ductus arteriosus, atrial and ventricular septal defect and bilateral mild ventriculomegaly in the neonatal period. However, as the baby grew older, the typical facial features became more prominent, and overall developmental delays were noted at the subsequent follow-up, with the motor and cognitive development significantly lagging behind that of other children of the same age. At 26 days old, laboratory tests revealed remarkably elevated levels of serum homocysteine, C3/C2 and urine organic acid. Whole-exome sequencing detected compound heterozygous variants in MMACHC, including one previously reported mutation [c.609G > A (p.W203X) and a novel missense mutation[ c.643 T > C (p.Y215H)]. The computer simulations of the protein structure analysis of the novel missense mutation showed the variant p.Y215H replaced a neutral amino acid with a strongly basic lysine, which broken the local structure by changing the carbon chain skeleton and decreasing the interaction with adjacent amino acid. This is expected to damage the utilization of vitamin B12 and influence the synthesis of AdoCbl and MeCbl, contributing to its pathogenicity. Thus, clinical and genetic examinations confirmed the cblC disease. Another heterozygous variant in ZEB2 [NM_014795; loss1(exon:2-10)(all); 127901 bp] was detected by whole-exome sequencing. The heterozygous 3.04 Mb deletion in EB2 [GRCH37]del(2)(q22.2q22.3) (chr2:142237964-145274917) was also confirmed by genome-wide copy number variations (CNVs) scan, which was pathogenic and led to the diagnosis of Mowat-Wilson syndrome. The biochemical indicators associated with cblC disease in the patient were well controlled after treatment with vitamin B12 and betaine. Here, a patient with coexisting cblC disease and MWS caused by different pathogenic genes was reported, which enriched the clinical research on these two rare genetic diseases.
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http://dx.doi.org/10.1016/j.cca.2022.06.004DOI Listing
June 2022
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