Publications by authors named "Fang Li"

3,169 Publications

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CsAAP7.2 is involved in the uptake of amino acids from soil and the long-distance transport of theanine in tea plants (Camellia sinensis L.).

Tree Physiol 2022 Jun 28. Epub 2022 Jun 28.

College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

Tea plant roots can uptake both inorganic nitrogen (NH4+ and NO3-) and organic nitrogen (amino acids) from soil. These amino acids are subsequently assimilated into theanine and transported to young shoots through xylem. Our previous study showed that CsLHT1 and CsLHT6 transporters take up amino acids from soil, and CsAAPs participate in the transport of theanine. However, whether other amino acid transporters are involved in this process remains unknown. In this study, we identified two new CsAAPs homologous to CsAAP7, named CsAAP7.1 and CsAAP7.2. Heterologous expression of CsAAP7.1 and CsAAP7.2 in the yeast mutant 22Δ10α showed that CsAAP7.2 had the capacity to transport theanine and other amino acids, while CsAAP7.1 had no transport activity. Transient expression of the CsAAP7.2-GFP fusion protein in tobacco leaf epidermal cells confirmed its localization to the endoplasmic reticulum. Tissue-specific analysis showed that CsAAP7.2 was highly expressed in roots and stems. In addition, CsAAP7.2 overexpression lines were more sensitive to high concentrations of theanine due to the high accumulation of theanine in seedlings. Taken together, these findings suggested CsAAP7.2 plays an important role in the uptake of amino acids from soil and the long-distance transport of theanine. These results provide valuable tools for N nutrition studies and enrich our understanding of theanine transport in tea plants.
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http://dx.doi.org/10.1093/treephys/tpac071DOI Listing
June 2022

Risk Factors and Prognostic Follow-Up of Vasovagal Syncope Children With Seizure-Like Activities During Head-Up Tilt Test Induced-Syncope.

Front Cardiovasc Med 2022 10;9:916542. Epub 2022 Jun 10.

Department of Pediatric Cardiovasology, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.

Objectives: To analyze the risk factors associated with seizure-like activities during head up tilt test (HUTT)-induced syncope in children with vasovagal syncope (VVS) and assess the prognosis of these patients.

Methods: This is a retrospective study. VVS children with or without seizure-like activities during HUTT-induced syncope were included in convulsive or non-convulsive group. The clinical characteristics, hemodynamic parameters during HUTT-induced syncope and follow-up data were reviewed from the HUTT case report form and analyzed.

Results: 68 cases (25 males, mean age 11.86 ± 3.35 years) were enrolled in convulsive group and 65 cases in non-convulsive group (24 males, mean age 11.64 ± 2.11 years). There were statistical differences in history duration, response type, and asystole between the two groups (all < 0.05). Fully adjusted logistic regression showed that the risk of seizure-like activities was increased by 37.18 folds for patients with asystole compared with those without asystole ( = 0.005), by 308.25 and 6.08 folds for patients with cardioinhibitory type or mixed type compared with vasoinhibitory type ( < 0.01). No significant difference was exhibited in negative HUTT conversion rate and the proportion of re-syncope patients between the two groups at follow-up (both > 0.05). None of these convulsive patients underwent pacemaker implantation during follow-up.

Conclusions: Asystole and response type were independent risk factors associated with seizure-like activities. Patients with asystole and mixed or cardioinhibitory responses to HUTT should be closely concerned. However, VVS children with seizure-like activities did not have a poor prognosis at follow-up.
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http://dx.doi.org/10.3389/fcvm.2022.916542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226399PMC
June 2022

Acute SGLT-2i treatment improves cardiac efficiency during myocardial ischemia independent of Na+/H+ exchanger-1.

Int J Cardiol 2022 Jun 23. Epub 2022 Jun 23.

Department of Integrative Medical Sciences, Northeast Ohio Medical University, Rootstown, OH, United States of America. Electronic address:

Aims: Sodium glucose co-transporter 2 inhibitors (SGLT2i) demonstrate cardioprotective benefits independent of a glucose lowering effect including preservation of cardiac function during a myocardial ischemia. Sodium‑hydrogen exchanger-1 (NHE-1), has been hypothesized to contribute to the cardiac effects of SGLT2i. We characterized the beneficial effects of acute pre-ischemia exposure to SGLT2i and explored the possibility that these effects are explained by NHE-1 inhibition.

Methods And Results: Swine were anesthetized and instrumented for invasive hemodynamic measurements After baseline data collection, swine received a 15-30 min intravenous infusion of vehicle (DMSO), the SGLT2i canagliflozin (~1 mg/kg), or the NHE-1 inhibitor cariporide (~0.03 mg/kg) ending immediately prior to occlusion of the left circumflex artery. Measurements were obtained at baseline, during a 60-min complete occlusion of the circumflex coronary artery, and during a 2-h reperfusion period. Blood pressure, heart rate, left anterior descending artery flow, and associated myocardial oxygen consumption were unaffected by acute pre-treatment with canagliflozin or cariporide during ischemia and reperfusion. Acute pre-ischemic treatment with canagliflozin significantly increased diastolic filling and stroke work, producing a rightward shift in the Frank-Starling relationship, and also improved cardiac work efficiency relative to untreated control hearts during ischemia. Effects of NHE-1 inhibition with cariporide were modest and dissimilar. Examination of AP-1 cells transfected with wild-type NHE-1 and iPSC-derived cardiomyocytes confirmed dose-dependent-inhibition of NHE-1 activity by cariporide, while canagliflozin had no significant effect on NHE-1 activity.

Conclusion: Acute pre-treatment with SGLT2i produces cardioprotective effects during ischemia, including improved work efficiency. These effects are not explained by NHE-1 inhibition.

Translational Perspective: SGLT2 inhibitors have been shown to improve cardiac outcomes in patient including reducing myocardial infarction incidence and mortality. The mechanism(s) explaining this effect are not clear. This manuscript demonstrates a protective effect from acute SGLT2i exposure, as short as 15 min, prior to experimental infarction in swine. These effects were independent of NHE1 inhibition. These observations suggest that SGLT2 inhibitors can confer cardioprotective effects on a very short time scale. It is possible that such effects provide an ongoing contribution to ischemic protection even in the setting of chronic treatment.
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http://dx.doi.org/10.1016/j.ijcard.2022.06.054DOI Listing
June 2022

Can agricultural land use alter the responses of soil biota to antibiotic contamination?

J Hazard Mater 2022 Jun 11;437:129350. Epub 2022 Jun 11.

School of Ecology and Environmental Sciences, Yunnan University, Kunming 650500, China; State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Antibiotics accumulate in soils via various agricultural activities, endangering soil biota that play fundamental roles in maintaining agroecosystem function. However, the effects of land-use heterogeneity on soil biota tolerance to antibiotic stresses are not well understood. In this study, we explored the relationships between antibiotic residues, bacterial communities, and earthworm populations in areas with different land-use types (forest, maize, and peanut fields). The results showed that antibiotic levels were generally higher in maize and peanut fields than in forests. Furthermore, land use modulated the effects of antibiotics on soil bacterial communities and earthworm populations. Cumulative antibiotic concentrations in peanut fields were negatively correlated with bacterial diversity and earthworm abundance, whereas no significant correlations were detected in maize fields. In contrast, antibiotics improved bacterial diversity and richness in forest soils. Generally, earthworm populations showed stronger tolerance to antibiotics than did soil bacterial communities. Agricultural land use differentially modified the responses of the soil bacterial community and earthworm population to antibiotic contamination, and earthworms might provide an alternative for controlling antibiotic contamination.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129350DOI Listing
June 2022

First Report of Anthracnose Caused by on Spotted laurel in China.

Plant Dis 2022 Jun 24. Epub 2022 Jun 24.

Jiangxi Agricultural University, 91595, Key Laboratory of State Forestry Administration on Forest Ecosystem Protection and Restoration of Poyang Lake Watershed (JXAU), Nanchang, Jiangxi, China;

Spotted laurel (Aucuba japonica 'Variegata') is an evergreen shrub native to China, Korea and Japan, prized for its foliage of green and golden yellow mottled foliage (Fang and Hu 1990). In November 2020, about 50% of spotted laurel in Jiangxi Academy of Forestry (28°44'10''N, 115°49'1.62"E) at Jiangxi province were observed to have anthracnose-like symptoms. The typical symptoms were tended to coalesce to form initially dark brown specks on the leaves, which developed to nearly circular spots of the diameter no more than 1.2 cm and might join to large irregular spots. The spots were grayish white at the center, purple brown at the border and surrounded by a yellow halo. To isolate and identify the pathogen, 15 leaves with typical symptoms were sampled. Isolation and morphological analysis were performed following the method of Ding et al. (2021). Among 40 fungal isolates, 33 showed the same morphological characters. The colony on PDA was umbonate pink-gray in the center surrounding by white margin, the reverse was greyish-cream. The average mycelial growth rate was ca. 0.8 mm per day at 25±1°C on PDA. The conidia were hyaline, aseptate, straight, apex round and base round, mean ± SD = 16.50±3.75 μm × 7.50±2.50 μm. For further confirmation of the identity, six genes, including ITS, GAPDH, ACT, TUB2, CAL and CHS-1 (Damm et al. 2012) were amplified and sequenced. The sequences of rDNA-ITS, GAPDH, ACT, TUB2, CAL and CHS-1 of 1SJ5 were deposited in GenBank as: OM988385, ON009367-ON009371. Phylogenetic analysis based on the above six genes showed that isolates formed a single clade with the strains of Colletotrichum boninense. Pathogenicity tests of isolate 1SJ5 were carried out on the leaves in the field. The mycelial plugs of isolate 1SJ5 were applied on punctured leaves of A. japonica using a sterile needle in field. Inoculation with only a PDA plug served as controls. 14 -21 days, symptoms like those observed in the field, developed on the inoculated plants but not on the controls. The fungus was re-isolated from the margins of the leaf spots and identified by morphological and molecular characters. C. boninense has been reported as causing anthracnose on a broad range of hosts including strawberry (Bi et al. 2017), Eucalyptus robusta (Zhang and Zhu 2018), Alcantarea imperialis (Meneses et al. 2019), and so on. To our knowledge, this is the first report of leaf anthracnose on A. japonica caused by C. boninense in China and our findings will be useful for its management.
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http://dx.doi.org/10.1094/PDIS-03-22-0691-PDNDOI Listing
June 2022

Transesterification of Glycerol to Glycerol Carbonate over Mg-Zr Composite Oxide Prepared by Hydrothermal Process.

Nanomaterials (Basel) 2022 Jun 8;12(12). Epub 2022 Jun 8.

Key Laboratory of Green Chemical Technology and High Efficient Energy Saving of Hebei Province, Tianjin Key Laboratory of Chemical Process Safety, School of Chemical Engineering, Hebei University of Technology, Tianjin 300401, China.

A series of Mg-Zr composite oxide catalysts prepared by the hydrothermal process were used for the transesterification of glycerol (GL) with dimethyl carbonate (DMC) to produce glycerol carbonate (GC). The effects of the preparation method (co-precipitation, hydrothermal process) and Mg/Zr ratio on the catalytic performance were systematically investigated, and the deactivation of the catalyst was also explored. The Mg-Zr composite oxide catalysts were characterized by XRD, TEM, TPD, N adsorption-desorption, and XPS. The characterization results showed that compared with the co-precipitation process, the catalyst prepared by the hydrothermal process has a larger specific surface area, smaller grain size, and higher dispersion. Mg1Zr2-HT catalyst calcined at 600 °C in a nitrogen atmosphere exhibited the best catalytic performance. Under the conditions of reaction time of 90 min, reaction temperature of 90 °C, catalyst dosage of 3 wt% of GL, and GL/DMC molar ratio of 1/5, the GL conversion was 99% with 96.1% GC selectivity, and the yield of GC was 74.5% when it was reused for the fourth time.
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http://dx.doi.org/10.3390/nano12121972DOI Listing
June 2022

Morphometric Characteristic of Black Soldier Fly () · Wuhan Strain and Its Egg Production Improved by Selectively Inbreeding.

Life (Basel) 2022 Jun 10;12(6). Epub 2022 Jun 10.

State Key Laboratory of Agricultural Microbiology, National Engineering Research Centre of Microbial Pesticides, College of Life Science and Technology, College of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.

The use of black soldier fly (BSF) larvae to recycle various organic materials while producing biomass for use as feed is well established. Variety selection is important from the perspective of application. In the current study, morphometric and life-history traits of a Wuhan-domesticated BSF colony (Wuhan strain) were compared to those of a 'selectively inbred' population (inbred strain, inbred for 10 generations). In terms of morphological characteristics, the results showed that both strains had dichoptic compound eyes, club-shaped antennae, blue halters, and blue-green metallic luster wings with a hexagon discal cell. In both strains, the body and wing length of female adults were slightly larger than those of male adults. The first four larval stages of the BSF occurred rapidly (1-12 days) with transitions across stages resulting in doubling of size for both populations. Selective inbreeding did not alter the life-history traits of the larval exuviate stage in terms of age, size, weight, and feed reduction rate. Overall egg production for the inbred strain was significantly higher (1.5 times greater) than the Wuhan strain. This is explained by increased adult emergence and individual oviposition performance. It was speculated that inbreeding improved the reproductive success of inbred adult female offspring and selection process steadied it. The findings indicate that selective inbreeding could enhance overall oviposition performance and provide a strategy to selectively breed BSF with high egg production for future applications.
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http://dx.doi.org/10.3390/life12060873DOI Listing
June 2022

O-Fluorobenzoic Acid-Mediated Construction of Porous Graphitic Carbon Nitride with Nitrogen Defects for Multicolor Electrochemiluminescence Imaging Sensing.

Anal Chem 2022 Jun 23. Epub 2022 Jun 23.

CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

Graphitic carbon nitride (g-CN) is an attractive electrochemiluminescence (ECL) luminophore. However, g-CN with wavelength-tunable ECL emission is still limited, which limits its application in multicolor ECL sensing and imaging analysis. In this study, porous g-CN (PCN) with nitrogen defects was synthesized through the condensation of melamine by using -fluorobenzoic acid (-FBA) as an effective regulation reagent. A series of PCNs, including PCN-5%, PCN-10%, and PCN-30%, were obtained by changing the mass ratio of -FBA and melamine. The porous structure and tunable chemical composition change of the PCNs were carefully characterized. The nitrogen defects and porous structure of the synthesized PCNs can enlarge the specific surface area, facilitate electron transfer, and generate various surface states with gradually changed energy bands, leading to wavelength-tunable multicolor ECL emissions. Accordingly, g-CN, PCN-5%, PCN-10%, and PCN-30% can generate navy blue, turquoise blue, turquoise green, and olive green ECL emissions, respectively, with the peak ECL wavelength varied from 465 to 550 nm. Then, a multicolor ECL sensing array was proposed for the discrimination of polyphenols based on the prepared g-CN and PCNs by using a smartphone as a portable detector for the first time. Five polyphenol substances including vitamin P, resveratrol, phloretin, phlorizin, and caffeic acid were discriminated by using principal component analysis and hierarchical cluster analysis. The present work provides a simple strategy to adjust the ECL wavelength of g-CN and presents a simple way to fabricate multicolor ECL sensing array, which has great application potential for multiplexed analysis and multicolor ECL imaging sensing.
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http://dx.doi.org/10.1021/acs.analchem.2c00702DOI Listing
June 2022

Fasting Enhances the Acute Toxicity of Acrylonitrile in Mice via Induction of CYP2E1.

Toxics 2022 Jun 19;10(6). Epub 2022 Jun 19.

Department of Preventive Medicine and Public Health Laboratory Science, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.

Cytochrome P450 2E1 (CYP2E1) plays an essential role in the susceptibility to acute acrylonitrile (AN)-induced toxicity. Here, we investigated the toxicity and mechanism of AN in fasting mice and potential underlying mechanisms. Convulsions, loss of righting reflex, and death 4 h after AN treatment were observed and recorded for each group of mice. Relative to ad lib-fed mice, 48 h fasting significantly increased the acute toxicity of AN, as noted by a more rapid onset of convulsions and death. In addition, fasting significantly enhanced CYP2E1-mediated oxidative metabolism of AN, resulting in increased formation of CN (one of the end-metabolites of AN). Moreover, fasting decreased hepatic GSH content, abrogating the detoxification of GSH. However, trans-1,2-dichloroethylene (DCE), a CYP2E1 inhibitor, altered the level of hepatic CYP2E1 activity in response to fasting, reduced the acute toxic symptoms of AN and the content of CN in AN-treated mice. These data establish that fasting predisposes to AN toxicity, attributable to induced CYP2E1 and reduced hepatic GSH.
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http://dx.doi.org/10.3390/toxics10060337DOI Listing
June 2022

A Dynamic Nomogram for Predicting Survival among Diabetic Patients on Maintenance Hemodialysis.

Ther Apher Dial 2022 Jun 22. Epub 2022 Jun 22.

Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.

Introduction: Amongst maintenance hemodialysis (MHD) patients, ones with diabetes mellitus (DM) are known to have the worst outcome.

Methods: A total of 263 MHD patients were included,a dynamic nomogram was established based on multivariable Cox regression analysis.

Results: The median overall survival (OS) time was 46 months. The 1-, 3- and 5-year OS rates were 90.9%, 70.5% and 53.9%, respectively. The multivariable Cox regression analysis indicated that DM duration, cardiovascular complication, baseline values before starting MHD for estimated glomerular filtration rate and serum phosphate were independent risk factors. The C-index of the dynamic nomogram was 0.745 and the calibration curves showed optimal agreement between the model prediction and actual observation for predicting survival probabilities.

Conclusions: Our study was the first to establish dynamic nomogram among diabetic MHD patients, the fast and convenient online tool can be used for individual risk estimation at the point of prognosis prediction. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1744-9987.13901DOI Listing
June 2022

Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer.

Cancer Immunol Immunother 2022 Jun 20. Epub 2022 Jun 20.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 201240, China.

Immunosuppression induced by myeloid-derived suppressor cells (MDSCs) is one of the main obstacles to the efficacy of immunotherapy for cervical cancer. Recent studies on the immunosuppressive ability of MDSCs have primarily focused on T cells, but the effect of MDSCs on B cells function is still unclear. In a study of clinical specimens, we found that the accumulation of MDSCs in patients with cervical cancer was accompanied by high expression of B cell activating factor (BAFF) on the surface and high expression of interleukin (IL)-10-producing B cells (B10) in vivo. We found that the absence of BAFF could significantly inhibit tumor growth in a cervical cancer model using BAFF KO mice. Further studies showed that abundant MDSCs in cervical cancer induced B cells to differentiate into B10 cells by regulating BAFF which acted on the BAFF receptor (BAFF-R) of them. In this process, we found that a large amount of IL-10 secreted by B10 cells can activate STAT3 signaling pathway in MDSCs, and then form a positive feedback loop to promote the differentiation of B10 cells. Therefore, this study reveals a new mechanism of BAFF-mediated mutual immune regulation between MDSCs and B cells in the occurrence and development of cervical cancer.
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http://dx.doi.org/10.1007/s00262-022-03226-0DOI Listing
June 2022

Oxidative injury induced by drinking water disinfection by-products dibromoacetonitrile and dichloroacetonitrile in mouse hippocampal neuronal cells: the protective effect of N-acetyl-L-cysteine.

Toxicol Lett 2022 Jun 17. Epub 2022 Jun 17.

School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address:

Dibromoacetonitrile (DBAN) and dichloroacetonitrile (DCAN) are haloacetonitriles (HANs) produced as by-products of chloramine disinfection of drinking water and can cause neurotoxicity. The molecular pathways leading to HAN-induced neuronal cell death remain unclear. The nuclear factor erythroid 2-related factor 2 (Nrf2) is an important regulator of oxidation reactions. We explored the role of the sequestosome 1 (p62)-Kelch-like ECH-associated protein 1 (Keap1)-Nrf2 pathway in DBAN- and DCAN-induced mouse hippocampal neuronal (HT22) cell injury. DBAN and DCAN reduced cell viability, increased lactate dehydrogenase release rate, and promoted apoptosis. Over the same treatment time, DBAN at lower concentrations caused cell injury, suggesting that DBAN is more cytotoxic than DCAN. DBAN and DCAN triggered oxidative stress by reducing intracellular glutathione and increasing reactive oxygen species concentrations. DBAN and DCAN activated the Nrf2 pathway. Furthermore, Nrf2 inhibitors (all-trans retinoic acid) attenuated DBAN- and DCAN-induced toxicity, whereas Nrf2 activators (tert-Butylhydroquinone) achieved the opposite effect. This indicates that activation of the Nrf2 pathway mediates DBAN- and DCAN-induced cell injury. Notably, the expression of p62, a noncanonical pathway that mediates Nrf2 activation, increased, whereas the expression of Keap1, another regulator of Nrf2, decreased. We noted that high p62 expression activated the Nrf2 pathway, and p62 was regulated through Nrf2, forming a positive feedback loop. N-acetyl-L-cysteine, a mercaptan substance, protected against DBAN- and DCAN-induced toxicity and inhibited the Nrf2 pathway. In summary, Nrf2 pathway inhibition and mercaptan supplementation prevent DBAN- and DCAN-induced HT22 cell injury, accordingly, targeting them is a potential approach to preventing HAN-induced neurotoxicity.
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http://dx.doi.org/10.1016/j.toxlet.2022.06.005DOI Listing
June 2022

The Relationship Between Unexplained Chest Pain in Children and Head-Up Tilt Test.

Front Pediatr 2022 2;10:901919. Epub 2022 Jun 2.

Department of Pediatric Cardiovasology, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: To explore the relationship between unexplained chest pain in children and head-up tilt test (HUTT).

Materials And Methods: A total of 825 children with the main symptom of unexplained chest pain were admitted to the Specialist Outpatient Clinic of Children's Cardiovascular Disease from October 2000 to November 2021 at The Second Xiangya Hospital, Central South University. Among them, 473 were male and 352 were female, with a mean age of 10.61 ± 2.21 years. The control group included 58 cases, comprising 35 males and 23 females, with a mean age of 10.26 ± 2.66 years. The detailed history, physical examinations, conventional 12-lead electrocardiogram, chest X-ray, echocardiography, myocardial enzymes, electroencephalogram, and blood series were all examined. Disorders of the chest wall, lung, heart, mediastinum, and esophageal reflux, as well as drug effects, were ruled out. All the children underwent HUTT. Demographic description, univariate analysis, and multivariate logistic regression analysis were used to explore the possible linear or non-linear relationships between the children's unexplained chest pain and HUTT.

Results: Among the 825 chest pain cases, 301 (36.48%) were HUTT positive and 524 (63.52%) were HUTT negative. HUTT-positive patients were older than HUTT-negative patients (11.04 ± 2.03 vs. 10.34 ± 2.31 years, < 0.001). The logistic regression results showed that each year of age increased the probability of being HUTT positive by 17.90% ( < 0.000), and females were 91.30% more likely to be HUTT positive than males ( < 0.000).

Conclusion: Clinically unexplained chest pain in children is mainly caused by unbalanced autonomic nervous function. HUTT can help clear the cause of unexplained chest pain.
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http://dx.doi.org/10.3389/fped.2022.901919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203148PMC
June 2022

Potential Markers of Neurocognitive Disorders After Cardiac Surgery: A Bibliometric and Visual Analysis.

Authors:
Linna Ji Fang Li

Front Aging Neurosci 2022 1;14:868158. Epub 2022 Jun 1.

Department of Neurology, Fu Xing Hospital, Capital Medical University, Beijing, China.

Background: Identifying useful markers is essential for diagnosis and prevention of perioperative neurocognitive disorders (PNDs). Here, we attempt to understand the research basis and status, potential hotspots and trends of predictive markers associated with PNDs after cardiac surgery via bibliometric analysis.

Methods: A total of 4,609 original research articles and reviews that cited 290 articles between 2001 and 2021 were obtained from the Web of Science Core Collection (WoSCC) as the data source. We used the software CiteSpace to generate and analyze visual networks of bibliographic information, including published years and journals, collaborating institutions, co-cited references, and co-occurring keywords.

Results: The number of annual and cumulative publications from 2001 to 2021 has been increasing on the whole. The Harvard Medical School was a very prolific and important institution in this field. The journal of Ann Thorac Surg (IF 4.33) had the most publications, while New Engl J Med was the most cited journal. Neuron-specific enolase (NSE), S100b and kynurenic acid (KYNA) were frequently discussed as possible markers of PNDs in many references. Cardiopulmonary bypass (CPB) was a keyword with high frequency (430) and sigma (6.26), and inflammation was the most recent burst keyword.

Conclusion: Potential markers of PNDs has received growing attention across various disciplines for many years. The research basis mainly focuses on three classic biomarkers of S100b, NSE, and KYNA. The most active frontiers are the inflammation-related biomarkers (e.g., inflammatory cells, cytokines, or mediators) and surgery-related monitoring parameters (e.g., perfusion, oxygen saturation, and the depth of anesthesia).
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http://dx.doi.org/10.3389/fnagi.2022.868158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199578PMC
June 2022

PrecisionFDA Truth Challenge V2: Calling variants from short and long reads in difficult-to-map regions.

Cell Genom 2022 May 27;2(5). Epub 2022 Apr 27.

The Canadian Center for Computational Genomics (C3G), Montréal, QC, Canada.

The precisionFDA Truth Challenge V2 aimed to assess the state of the art of variant calling in challenging genomic regions. Starting with FASTQs, 20 challenge participants applied their variant-calling pipelines and submitted 64 variant call sets for one or more sequencing technologies (Illumina, PacBio HiFi, and Oxford Nanopore Technologies). Submissions were evaluated following best practices for benchmarking small variants with updated Genome in a Bottle benchmark sets and genome stratifications. Challenge submissions included numerous innovative methods, with graph-based and machine learning methods scoring best for short-read and long-read datasets, respectively. With machine learning approaches, combining multiple sequencing technologies performed particularly well. Recent developments in sequencing and variant calling have enabled benchmarking variants in challenging genomic regions, paving the way for the identification of previously unknown clinically relevant variants.
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http://dx.doi.org/10.1016/j.xgen.2022.100129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205427PMC
May 2022

The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury inducedB19 by LPS via NMMHC IIA/Wnt5a/β-catenin pathway.

Toxicol Appl Pharmacol 2022 Jun 15:116132. Epub 2022 Jun 15.

State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address:

Acute lung injury (ALI) or its most advanced form, acute respiratory distress syndrome (ARDS), is a severe inflammatory pulmonary process triggered by varieties of pathophysiological factors, among which endothelial barrier disruption plays a critical role in the progression of ALI/ARDS. As an inhibitor of myosin II, blebbistatin inhibits endothelial barrier damage. This study aimed to investigate the effect of blebbistatin on lung endothelial barrier dysfunction in LPS induced acute lung injury and its potential mechanism. Mice were challenged with LPS (5 mg/kg) by intratracheal instillation for 6 h to disrupt the pulmonary endothelial barrier in the model group. Blebbistatin (1 mg/kg, ip) was administrated 1 h before LPS challenge. The results showed that blebbistatin could significantly attenuate LPS-induced lung injury and pulmonary endothelial barrier dysfunction. And we observed that blebbistatin inhibited the activation of NMMHC IIA/Wnt5a/β-catenin pathway in pulmonary endothelium after LPS treatment. In murine lung vascular endothelial cells (MLECs) and human umbilical vein endothelial cells (HUVECs), we further confirmed that Blebbistatin (1 μmol/L) markedly ameliorated endothelial barrier dysfunction in MLECs and HUVECs by modulating NMMHC IIA/Wnt5a/β-catenin pathway. Our data demonstrated that blebbistatin could inhibit the development of pulmonary endothelial barrier dysfunction and ALI via NMMHC IIA/Wnt5a/β-catenin signaling pathway.
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http://dx.doi.org/10.1016/j.taap.2022.116132DOI Listing
June 2022

X-Linked Inhibitor of Apoptosis Protein (XIAP)-Loaded Magnetic Mesoporous Silica Nanoparticles Incorporated with miR-233 to Improve Radio Sensitization of Cervical Cancer Cells and Promote Apoptosis.

J Biomed Nanotechnol 2022 Mar;18(3):747-753

Department of Radiation Oncology, The Second Affiliated Hospital of Xian Jiaotong University, Xi'an City, Shanxi Province, 710003, China.

This study investigated the impact of magnetic mesoporous silica nanoparticles (MMSN)-encapsulated X-linked inhibitor of apoptosis protein (XIAP) and miR-233 on tumor microenvironment in cervical cancer, to provide targeted treatment and strategy, to improve radio sensitization of cancer cells. Cervical cancer cells were treated with normal saline (control group), XIAP-loaded metallic mesoporous silica nanoparticles (MMSNs), and miR-233-targeted material (XIAP group, XIAP+miR-233 group). Proliferation, apoptosis and colony forming ability of cancer cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method, flow cytometry and colony formation experiments. experiments were established to observe the impact of XIAP-loaded MMSNs and miR-233 on tumor growth. Administration of XIAP-loaded MMSNs suppressed tumor growth of cervical cancer, and presence of miR-233 targeted material further decreased tumor volume, increasing radio sensitization of cancer cells. experiments confirmed that, combined treatment of XIAP and miR-233 suppressed cancer cell proliferation and invasion when inducing apoptosis. XIAP MMSNs characterized by large unit surface area, high dispersion and adhesion, and prolonged circulation time, improving drug delivery and treatment selectivity of chemotherapeutic drugs. This study suggests that XIAP MMSNs with miR-233 material suppress cervical cancer cell progression and tumor growth when augmenting radiosensitization of cancer cells, providing evidence for targeted therapy for the disease.
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http://dx.doi.org/10.1166/jbn.2022.3281DOI Listing
March 2022

How does home ownership affect migrants' sense of relative deprivation? An investigation based on field theory.

Soc Sci Med 2022 Jun 1;306:115097. Epub 2022 Jun 1.

College of Public Administration, Nanjing Agricultural University, Nanjing, China. Electronic address:

Background: China is continuing to witness rising numbers of migrants (e.g., individuals migrating from rural to urban areas), and alongside this are the social restrictions and institutional barriers migrants face. Such restrictions and barriers are a consequence of the long-standing urban-rural dualist system and can create a sense of relative deprivation among migrants-that is, dissatisfaction when migrants perceive they are at a disadvantage compared with local residents of an area.

Objective And Method: Based on Pierre Bourdieu's field theory, the current study used data from the 2017 Chinese General Social Survey (N = 1849) to explore the mechanism through which migrants' home ownership or non-ownership in the migration process affects their sense of relative deprivation. To do so, a ranked regression and parallel multiple mediation model were developed. Additionally, a heterogeneity analysis was conducted to account for the region in which migrants lived and their age.

Results: The results revealed that home ownership significantly reduced migrants' relative deprivation. Moreover, the perception of economic and symbolic capital was found to play a role in the effects of wealth and class, respectively. From the heterogeneity analysis, the direct and mediated effects of housing attributes on migrants' relative deprivation were more significant for migrants in the eastern versus central and western regions of China, as well as among new-versus older-generation migrants.

Conclusion: To improve the feasibility of home ownership among migrants and, thus, alleviate their relative deprivation in the inflow area, relevant policies (e.g., improving the housing system pathway) should be developed and implemented.
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http://dx.doi.org/10.1016/j.socscimed.2022.115097DOI Listing
June 2022

Photocatalytic 1,2-oxo-alkylation reaction of styrenes with diazoacetates.

Chem Commun (Camb) 2022 Jun 15. Epub 2022 Jun 15.

RWTH Aachen University, Institute of Organic Chemistry, Landoltweg 1, D-52074 Aachen, Germany.

We report on the photocatalytic 1,2-difunctionalization reaction of styrenes with acceptor-only diazoalkanes. In the presence of DABCO and BuOOH, the carbene reactivity of diazoalkanes can be suppressed and a 1,2 oxo-alkylation reaction can be achieved (32 examples, up to 94% yield) without the formation of cyclopropane by-products the formation of radical intermediates from ethyl diazoacetate.
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http://dx.doi.org/10.1039/d2cc02414dDOI Listing
June 2022

Polysaccharides-based delivery system for efficient encapsulation and controlled release of food-derived active peptides.

Carbohydr Polym 2022 Sep 7;291:119580. Epub 2022 May 7.

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China. Electronic address:

A polysaccharides-based delivery system was designed to encapsulate and control the release of peanut peptide (PP). The PP-loaded polyelectrolyte complex (TMC-PP-SA) was fabricated based on the electrostatic self-assembly between n-trimethy chitosan (TMC) and sodium alginate (SA). The complex exhibited uniform spherical morphology, satisfactory stability and high encapsulation efficiency. In vitro release behavior indicated that TMC-PP-SA polyelectrolyte complex could inhibit the release of PP at simulated gastric medium and enhance the release of PP at simulated intestinal medium. Moreover, the antioxidant activity of PP after encapsulation was significantly improved compared with that of directly digested PP. Ex vivo intestinal permeation study confirmed that about 41.76 ± 1.43% PP in TMC-PP-SA could be absorbed in the intestinal. The cytotoxicity measurement indicated that the fabricated TMC-PP-SA polyelectrolyte complex was biocompatible and nontoxic. Therefore, these results indicated that the polysaccharides-based delivery system had great potential in protecting active peptides from degradation and facilitating their absorption.
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http://dx.doi.org/10.1016/j.carbpol.2022.119580DOI Listing
September 2022

Intraspecific Identification of Lingzhi Medicinal Mushroom, Ganoderma lingzhi (Agaricomycetes), Using ISSR Markers.

Int J Med Mushrooms 2022 ;24(4):43-52

Sichuan Key Laboratory of Quality and Innovation of Traditional Chinese Medicine, Institute of Fungus Medicinal Materials, Sichuan Academy of Chinese Medicine Science, Chengdu, 610041 Sichuan Province, People's Republic of China.

This study aimed to obtain a set of specific inter simple sequence repeat (ISSR) primers and establish a stable and accurate intraspecific identification method for Ganoderma lingzhi. A total of 117 G. lingzhi strains were identified using internal transcribed spacer sequences from 147 strains determined as G. lingzhi via simple morphological identification. Based on the sequences obtained, specific ISSR primers for G. lingzhi were screened and validated, and 15 specific ISSR primers showed polymorphic banding pattern with clear band resolution. Subsequently, ISSR PCRs of the 15 specific primers were performed for the 117 G. lingzhi strains. As expected, DNA analysis of the ISSR markers could distinguish G. lingzhi strains, with similarity coefficients ranging from 0.11 to 0.89. Thus, the 15 specific ISSR primers can be used for intraspecific identification and polymorphism analysis of G. lingzhi.
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http://dx.doi.org/10.1615/IntJMedMushrooms.2022043141DOI Listing
June 2022

Influence of atmospheric teleconnections on interannual variability of Arctic-boreal fires.

Sci Total Environ 2022 Sep 8;838(Pt 4):156550. Epub 2022 Jun 8.

Woods Hole Research Center, Falmouth, MA, USA.

Fires across the Arctic-boreal zone (ABZ) play an important role in the boreal forest succession, permafrost thaw, and the regional and global carbon cycle and climate. These fires occur mainly in summer with large interannual variability. Previous studies primarily focused on the impacts of local surface climate and tropical El Niño-Southern Oscillation (ENSO). This study, for the first time, comprehensively investigates the influence of summer leading large-scale atmospheric teleconnection patterns in the Northern Hemisphere extra-tropics on interannual variability of ABZ fires. We use correlation and regression analysis of 1997-2019 multiple satellite-based products of burned area and observed/reanalyzed climate data. Results show that eight leading teleconnection patterns significantly affect 63 ± 2 % of burned areas across the ABZ. Western North America is affected by the East Pacific/North Pacific pattern (EP/NP) and the West Pacific pattern (WP); boreal Europe by the Scandinavia pattern (SCA); eastern North America, western and central Siberia, and southeastern Siberia by the North Atlantic Oscillation (NAO); and eastern Siberia /Russian Far East by the East Atlantic pattern (EA). NAO/EA induces lower-tropospheric drier northwesterly/northerly airflow passing through the east of boreal North America/Eurasia, which decreases surface relative humidity. Other teleconnections trigger a high-pressure anomaly, forcing downward motion that suppresses cloud formation and increases solar radiation reaching the ground to warm the surface air as well as brings drier air downward to reduce surface relative humidity. The drier and/or warmer surface air can decrease fuel wetness and thus increase burned area. Our study highlights the important role of the extra-tropical teleconnection patterns on ABZ fires, which is much stronger than ENSO that was thought to control interannual variability of global fires. It also establishes a theoretical foundation for ABZ fire prediction based on extra-tropical teleconnections, and has the potential to facilitate ABZ fire prediction and management.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156550DOI Listing
September 2022

Interleukin-6 neutralizing antibody attenuates the hypersecretion of airway mucus via inducing the nuclear translocation of Nrf2 in chronic obstructive pulmonary disease.

Biomed Pharmacother 2022 Aug 7;152:113244. Epub 2022 Jun 7.

Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, PR China; Key Laboratory of Respiratory Diseases Research and Medical Transformation of Anhui Province, Hefei 230022, Anhui, PR China. Electronic address:

Airway mucus hypersecretion is a vital pathophysiologic feature in chronic obstructive pulmonary disease (COPD) patients in which airflow limitation result, and it is key to strategizing in the management of COPD. To investigate the mechanisms underlying the action of interleukin-6 neutralizing antibody (IL-6 Ab) in attenuating airway mucus hypersecretion in COPD, human and mouse primary bronchial epithelial cells from COPD patients and mice were isolated, human organoid model of trachea was established and all treated with IL-6 and/or IL-6 Ab. The differential expression of Muc5ac and Nrf2 were determined in pDHBE compared to pNHBE cells via high-throughput sequencing of transcriptome. The serum concentration of Muc5ac was significantly elevated and positively correlated with IL-6 in COPD patients using ELISA, and the excessive mucus secretion was observed in the trachea of COPD patients using HE, AB-PAS and IHC staining. The levels of Muc5ac were significantly elevated in the IL-6-treated group, and diminished with IL-6 Ab treatment, both in vitro and in the organoid model using qRT-PCR, WB and IF. The expression levels of protein Muc5ac were significantly reduced in cells transfected with the IL-6 small interfering RNA (siRNA-IL-6), which was in contrast to the levels of protein Nrf2, and the protective effects of IL-6 Ab were inhibited in cells transfected with Nrf2 short hairpin RNA (shRNA-Nrf2). IL-6 Ab significantly attenuated hypersecretion of airway mucus by inducing nuclear translocation of Nrf2 in COPD. These findings indicated that IL-6 Ab may constitute a novel therapeutic agent for IL-6-induced airway mucus hypersecretion by improving airflow limitation in COPD patients.
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http://dx.doi.org/10.1016/j.biopha.2022.113244DOI Listing
August 2022

Activation of the TREK-1 Potassium Channel Improved Cognitive Deficits in a Mouse Model of Alzheimer's Disease by Modulating Glutamate Metabolism.

Mol Neurobiol 2022 Jun 9. Epub 2022 Jun 9.

School of Basic Medical Sciences, Lanzhou University, Lanzhou, 73000, Gansu, China.

Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by cognitive dysfunction. The glutamate (Glu) metabolic pathway may be a major contributor to the memory dysfunction associated with AD. The TWIK-related potassium channel-1 (TREK-1) protects against brain ischemia, but any specific role for the channel in AD remains unknown. In this study, we used SAMP8 mice as an AD model and age-matched SAMR1 mice as controls. We explored the trends of changes in TREK-1 channel activity and the levels of AD-related molecules in the brains of SAMP8 mice. We found that the expression level of TREK-1 increased before 3 months of age and then began to decline. The levels of Tau and Glu increased with age whereas the acetylcholine level decreased with age. α-Linolenic acid (ALA), an activator of the TREK-1 channel, significantly increased the TREK-1 level, and improved the learning and memory deficits of SAMP8 mice aged 6 months. The mechanism in play may involve the Glu metabolic pathway. After activation of the TREK-1 channel, damaged neurons and astrocytes were rescued, the levels of Glu and the N-methyl-D-aspartate receptor were downregulated, and the level of glutamate transporter-1 was upregulated. These findings suggest that TREK-1 plays a crucial role in the pathological progression of AD; activation of the TREK-1 channel improved cognitive deficits in SAMP8 mice via a mechanism that involved Glu metabolism. The TREK-1 potassium channel may thus be a valuable therapeutic target in AD patients.
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http://dx.doi.org/10.1007/s12035-022-02776-9DOI Listing
June 2022

Acute kidney injury in peripheral blood haploidentical hematopoietic stem cell transplantation: should attract enough attention.

Bone Marrow Transplant 2022 Jun 7. Epub 2022 Jun 7.

Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, 210029, China.

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http://dx.doi.org/10.1038/s41409-022-01731-5DOI Listing
June 2022

Application of a Machine Learning Algorithm in Generating an Evapotranspiration Data Product From Coupled Thermal Infrared and Microwave Satellite Observations.

Front Big Data 2022 20;5:768676. Epub 2022 May 20.

Center for Satellite Applications and Research (STAR), National Environmental Satellite, Data, and Information Service (NESDIS), National Oceanic and Atmospheric Administration (NOAA), College Park, MD, United States.

Land surface evapotranspiration (ET) is one of the main energy sources for atmospheric dynamics and a critical component of the local, regional, and global water cycles. Consequently, accurate measurement or estimation of ET is one of the most active topics in hydro-climatology research. With massive and spatially distributed observational data sets of land surface properties and environmental conditions being collected from the ground, airborne or space-borne platforms daily over the past few decades, many research teams have started to use big data science to advance the ET estimation methods. The Geostationary satellite Evapotranspiration and Drought (GET-D) product system was developed at the National Oceanic and Atmospheric Administration (NOAA) in 2016 to generate daily ET and drought maps operationally. The primary inputs of the current GET-D system are the thermal infrared (TIR) observations from NOAA GOES satellite series. Because of the cloud contamination to the TIR observations, the spatial coverage of the daily GET-D ET product has been severely impacted. Based on the most recent advances, we have tested a machine learning algorithm to estimate all-weather land surface temperature (LST) from TIR and microwave (MW) combined satellite observations. With the regression tree machine learning approach, we can combine the high accuracy and high spatial resolution of GOES TIR data with the better spatial coverage of passive microwave observations and LST simulations from a land surface model (LSM). The regression tree model combines the three LST data sources for both clear and cloudy days, which enables the GET-D system to derive an all-weather ET product. This paper reports how the all-weather LST and ET are generated in the upgraded GET-D system and provides an evaluation of these LST and ET estimates with ground measurements. The results demonstrate that the regression tree machine learning method is feasible and effective for generating daily ET under all weather conditions with satisfactory accuracy from the big volume of satellite observations.
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http://dx.doi.org/10.3389/fdata.2022.768676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163788PMC
May 2022

Low molecular weight heparin and pregnancy outcomes in women with inherited thrombophilia: A systematic review and meta-analysis.

J Obstet Gynaecol Res 2022 Jun 3. Epub 2022 Jun 3.

Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Hunan, China.

Aim: To determine whether the use of low molecular weight heparin (LMWH) improves the pregnancy outcomes in women with inherited thrombophilia by conducting a meta-analysis of randomized controlled trials and observational studies.

Methods: A systematic literature search of several databases was conducted through September 19, 2020 to identify relevant studies. The outcomes of interest included live birth and adverse pregnancy outcomes (APOs). The overall risk estimates were pooled using random-effects meta-analysis.

Results: Ten randomized controlled trials and 12 cohort studies were included. In the meta-analyses of randomized controlled trials, the effectiveness of LMWH-treatment was found to be statistically significant in decreasing the risk of APOs (risk ratio [RR] = 0.76; 95% confidence interval [CI]: 0.61-0.95; p = 0.020), rather than in increasing the rate of live birth (RR = 1.12; 95% CI: 0.93-1.34; p = 0.230). In the meta-analyses of cohort studies, results showed that the use of LMWH was associated with a significantly higher proportion of live birth (RR = 1.86; 95% CI: 1.09-3.19; p = 0.020) as well as a significantly lower ratio of APOs (RR = 0.46; 95% CI: 0.31-0.69; p < 0.001) in women with inherited thrombophilia.

Conclusions: The use of LMWH may have a potentially beneficial effect on reducing the risk of APOs and even increasing the live birth rate in women with inherited thrombophilia. Further well-designed clinical trials with large samples are needed to address the role of LMWH in improving pregnancy outcomes among pregnant women with inherited thrombophilia.
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http://dx.doi.org/10.1111/jog.15319DOI Listing
June 2022

Development of a Core-Shell Heterojunction TiO /SrTiO Electrolyte with Improved Ionic Conductivity.

Chemphyschem 2022 Jun;23(11):e202200314

Jiangsu Provincial Key Laboratory of Solar Energy Science and Technology, School of Energy & Environment, Southeast University, Nanjing, 210096, China.

The front cover artwork is provided by Prof. Faze Wang's group at the Southeast University. The built-in electric field created by the semiconductor heterostructure confines the proton transport on the surface layer of the nanocomposite core-shell heterostructure imparting faster ion transport and lower activation energy. Read the full text of the Research Article at 10.1002/cphc.202200170.
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http://dx.doi.org/10.1002/cphc.202200314DOI Listing
June 2022

Cancer-Secreted Exosomal MiR-620 Inhibits ESCC Aerobic Glycolysis FOXM1/HER2 Pathway and Promotes Metastasis.

Front Oncol 2022 16;12:756109. Epub 2022 May 16.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Esophageal squamous cell carcinoma (ESCC) is a leading cause of cancer death worldwide. MicroRNAs (MiRNAs) have been reported to regulate cell functions through exosomes. Through the Gene Expression Omnibus (GEO) database, miR-620 was selected as a serum miRNA highly expressed in ESCC, but its detailed role in ESCC has not been explored. Tumor-secreted miRNAs have been reported to promote cancer metastasis through reprogramming the aerobic glycolysis of lung fibroblasts. Therefore, we intended to verify whether exosomal miR-620 secreted in ESCC cells may regulate the aerobic glycolysis of lung fibroblasts.

Methods: The effect of miR-620 on the aerobic glycolysis of ESCC cells was firstly verified through bioinformatics prediction and mechanism assays. Exosomes secreted from ESCC cells was detected, and the influence of exosomal miR-620 in regulating the aerobic glycolysis of lung fibroblasts was then verified both and .

Results: MiR-620 inhibited ESCC malignancy and suppressed the aerobic glycolysis of ESCC cells targeting Forkhead box M1 (FOXM1) and human epidermal growth factor receptor 2 (HER2). Moreover, exosomal miR-620 was highly secreted in ESCC and could regulate HFL1 aerobic glycolysis FOXM1/HER2 signaling. Furthermore, exosomal miR-620 could promote ESCC metastasis by reprogramming the aerobic glycolysis of lung fibroblasts (HFL1).

Conclusion: Exosomal miR-620 secreted by ESCC cells inhibited the aerobic glycolysis FOXM1/HER2 axis and promoted cancer metastasis.
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http://dx.doi.org/10.3389/fonc.2022.756109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148961PMC
May 2022

Insights into the hippocampus proteome and phosphorylation modification alterations in C57BL/6 revealed the memory improvement mechanisms of a walnut-derived peptide.

Food Res Int 2022 Jun 30;156:111311. Epub 2022 Apr 30.

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China. Electronic address:

This work aimed to explore the underlying mechanisms of memory improvement effects of a walnut derived peptide WNP-10. The morris water maze test, combined with ultrastructural observation, hematoxylin and eosin and Nissl staining showed that WNP-10 significantly improved the learning and memory capability of the scopolamine-injured mice. The four-dimensional label-free quantification proteomics analysis identified 88 differentially expressed proteins in the WNP-10-treated group compared with scopolamine-induced impairment group. Pathway enrichment analysis and western blotting demonstrated that the WNP-10 can regulate the phosphatidylinositol-3-phosphate 5-kinase, cathepsin L, N-acetylgalactosamine 6-sulfate sulfatase and AP-3 complex subunit mu-1 expression to affect inositol phosphate metabolism, thereby maintaining lysosome homeostasis in scopolamine-injured mice. Notably, the results of phosphoproteomics demonstrated that WNP-10 administration resulted in the increased phosphorylation of phosphatidylinositol-3-phosphate 5-kinase. These findings provide novel insights into the underlying mechanism of memory improvement of walnut peptides.
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http://dx.doi.org/10.1016/j.foodres.2022.111311DOI Listing
June 2022
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