Publications by authors named "Fang Huang"

1,109 Publications

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Engineering living cells with cucurbit[7]uril-based supramolecular polymer chemistry: from cell surface engineering to manipulation of subcellular organelles.

Chem Sci 2022 Aug 6;13(30):8885-8894. Epub 2022 Jul 6.

South China Advanced Institute for Soft Matter Science and Technology, School of Emergent Soft Matter, South China University of Technology Guangzhou 510640 China

Supramolecular polymer chemistry, which closely integrates noncovalent interactions with polymeric structures, is a promising toolbox for living cell engineering. Here, we report our recent progress in exploring the applications of cucurbit[7]uril (CB[7])-based supramolecular polymer chemistry for engineering living cells. First, a modular polymer-analogous approach was established to prepare multifunctional polymers that contain CB[7]-based supramolecular recognition motifs. The supramolecular polymeric systems were successfully applied to cell surface engineering and subcellular organelle manipulation. By anchoring polymers on the cell membranes, cell-cell interactions were established by CB[7]-based host-guest recognition, which further facilitated heterogeneous cell fusion. In addition to cell surface engineering, placing the multifunctional polymers on specific subcellular organelles, including the mitochondria and endoplasmic reticulum, has led to enhanced physical contact between subcellular organelles. It is highly anticipated that the CB[7]-based supramolecular polymer chemistry will provide a new strategy for living cell engineering to advance the development of cell-based therapeutic materials.
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http://dx.doi.org/10.1039/d2sc02797fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350592PMC
August 2022

Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged H photoproduction in Chlamydomonas hpm91 mutant lacking proton gradient regulation 5 (PGR5).

Photosynth Res 2022 Aug 16. Epub 2022 Aug 16.

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Clean and sustainable H production is crucial to a carbon-neutral world. H generation by Chlamydomonas reinhardtii is an attractive approach for solar-H from HO. However, it is currently not large-scalable because of lacking desirable strains with both optimal H productivity and sufficient knowledge of underlying molecular mechanism. We hereby carried out extensive and in-depth investigations of H photoproduction of hpm91 mutant lacking PGR5 (Proton Gradient Regulation 5) toward its up-scaling and fundamental mechanism issues. We show that hpm91 is at least 100-fold scalable (up to 10 L) with continuous H collection of 7287 ml H/10L-HPBR in averagely 26 days under sulfur deprivation. Also, we show that hpm91 is robust and active during sustained H photoproduction, most likely due to decreased intracellular ROS relative to wild type. Moreover, we obtained quantitative proteomic profiles of wild type and hpm91 at four representing time points of H evolution, leading to 2229 and 1350 differentially expressed proteins, respectively. Compared to wild type, major proteome alterations of hpm91 include not only core subunits of photosystems and those related to anti-oxidative responses but also essential proteins in photosynthetic antenna, C/N metabolic balance, and sulfur assimilation toward both cysteine biosynthesis and sulfation of metabolites during sulfur-deprived H production. These results reveal not only new insights of cellular and molecular basis of enhanced H production in hpm91 but also provide additional candidate gene targets and modules for further genetic modifications and/or in artificial photosynthesis mimics toward basic and applied research aiming at advancing solar-H technology.
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http://dx.doi.org/10.1007/s11120-022-00945-4DOI Listing
August 2022

Alterations in the nasopharyngeal microbiota associated with active and latent tuberculosis.

Tuberculosis (Edinb) 2022 Jul 21;136:102231. Epub 2022 Jul 21.

Department of Clinical Laboratory, Air Force Medical Center, 30 Fucheng Road, Haidian District, Beijing, 100142, China. Electronic address:

Objective: To investigate the characteristic of nasopharyngeal microbiota at different states of Mycobacterium tuberculosis (MTB) infection.

Methods: Participants were recruited from a chest hospital and were divided into three groups: the active tuberculosis (ATB) group, the latent TB infection (LTBI) group and the healthy control (HC) group. Nasopharyngeal microbiota was analyzed by 16S rRNA sequencing and clinical laboratory test results of ATB patients were collected and statistically analyzed.

Results: Eleven ATB patients, 19 LTBI individuals and 18 healthy controls were included. Compared with LTBI group, Proteobacteria (P=0.04) and Gammaproteobacteria (P=0.01) increased in the ATB group. Compared with HC group, Pseudomonadales (P=0.03) and Moraxellaceae (P=0.04) increased, while Bacillales (P=0.04) and Lachnospiraceae (P=0.03) decreased in ATB group. Furthermore, Staphylococcus and Corynebacterium accounted for 70-80% in HC and LTBI groups. While in ATB group, they were less than 40%. Moreover, relative abundance of Corynebacterium, Corynebacteriaceae and Mycobacteriales was positively correlated with serum adenosine deaminase while negatively correlated with albumin, hemoglobin, and platelet counts in ATB patients.

Conclusions: The composition of nasopharyngeal microbiota changed significantly after MTB infection. The correlations between Corynebacterium and nutritional status (hemoglobin and albumin), immune-related molecules (adenosine deaminase) and inflammation-related indicators (platelet) in ATB patients deserve further exploration.
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http://dx.doi.org/10.1016/j.tube.2022.102231DOI Listing
July 2022

Enhancement of antibacterial and growth-promoting effects of Paenibacillus polymyxa by optimizing its fermentation process.

J Appl Microbiol 2022 Jul 30. Epub 2022 Jul 30.

Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Bioengineering, Wuhan Institute of Technology, Wuhan, China.

Aims: We aimed to enhance the antibacterial and growth-promoting effects of Paenibacillus polymyxa by improving the yield of spores, lipopeptides and indole-3-acetic acid (IAA) in the fermentation process.

Methods And Results: Through medium optimization by the response surface method and feeding fermentation, the number of spores reached 2.37 × 10  cfu ml with an increase of 38%, the content of lipopeptides reached 60.8 mg L with an increase of 89%, and the content of IAA reached 24.3 mg L with an increase of 176%, respectively, comparing with the original (un-optimized) culture conditions. The fermentation culture of P. polymyxa from the optimized medium and feeding fermentation resulted in higher colonization of P. polymyxa in soils than that from the original culture during the 49 days for testing. Comparing with the supernatant of the original culture, the supernatant of the P. polymyxa culture from the optimized medium and feeding fermentation showed enhanced antibacterial effects and plant growth-promoting effects. The enhanced antibacterial effect was shown as the increase of the inhibition zone by 59%, 45% and 26% against Ralstonia solanacearum, Erwinia carotovora and Xanthomonas campestris. The enhanced growth-promoting effects on tomato and strawberry plants were the increase of plant height by 47% and 5%, root length by 23% and 15% and root weight by 65% and 110%.

Conclusions: The combination of medium optimization and feeding fermentation effectively improved the yield of spores, lipopeptides and IAA. Lipopeptides and IAA lead to enhanced antibacterial and plant growth-promoting effects of the P. polymyxa product.

Significance And Impact Of This Study: The optimized fermentation method significantly improved the yield of spores, lipopeptides and IAA, thus providing theoretical and technical support for enhancing the antibacterial and growth-promoting effects of P. polymyxa products in agriculture.
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http://dx.doi.org/10.1111/jam.15750DOI Listing
July 2022

Identification and Characterization of a New Strain That Naturally Produces Significant Amount of Extracellular Laccase.

Front Microbiol 2022 18;13:878360. Epub 2022 Jul 18.

Aquatic and Crop Resource Development Research Centre, National Research Council, Ottawa, ON, Canada.

Natural biodegradation processes hold promises for the conversion of agro-industrial lignocellulosic biomaterials into biofuels and fine chemicals through lignin-degrading enzymes. The high cost and low stability of these enzymes remain a significant challenge to economic lignocellulosic biomass conversion. Wood-degrading microorganisms are a great source for novel enzyme discoveries. In this study, the decomposed wood samples were screened, and a promising γ-proteobacterial strain that naturally secreted a significant amount of laccase enzyme was isolated and identified as AORB19 based on its phenotypic and genotypic characteristics. The laccase activities in culture medium of strain AORB19 were confirmed both qualitatively and quantitatively. Significant cultural parameters for laccase production under submerged conditions were identified following a one-factor-at-a-time (OFAT) methodology: temperature 30°C, pH 9, yeast extract (2 g/l), Li, Cu, Ca, and Mn (0.5 mM), and acetone (5%). Under the selected conditions, a 6-fold increase (73.3 U/L) in laccase production was achieved when compared with the initial culturing conditions (12.18 U/L). Furthermore, laccase production was enhanced under alkaline and mesophilic growth conditions in the presence of metal ions and organic solvents. The results of the study suggest the promising potential of the identified strain and its enzymes in the valorization of lignocellulosic wastes. Further optimization of culturing conditions to enhance the AORB19 strain laccase secretion, identification and characterization of the purified enzyme, and heterologous expression of the specific enzyme may lead to practical industrial and environmental applications.
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http://dx.doi.org/10.3389/fmicb.2022.878360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339997PMC
July 2022

in the Elimination Phase - China, 2013-2020.

China CDC Wkly 2022 Jul;4(28):609-613

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, WHO Collaborating Center for Tropical Diseases, National Centre for International Research on Tropical Diseases, NHC Key Laboratory of Parasite and Vector Biology (National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention), Shanghai, China.

What Is Already Known About This Topic?: () was the most widely distributed and major human malaria parasite in China, considered the last parasite to be eliminated.

What Is Added By This Report?: The last domestic case was reported in 2016, while hundreds of imported cases were reported annually from 2013-2020, predominantly from Southeast Asia.

What Are The Implications For Public Health Practice?: In the post-elimination phase, adaptive and practical strategies focusing on imported cases should be updated and adopted to prevent malaria resurgence.
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http://dx.doi.org/10.46234/ccdcw2022.133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339366PMC
July 2022

Symptomatic Complete Discoid Medial Meniscus Completely Coalesced with the Anterior Cruciate Ligament: A Case Report and Literature Review.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

Center for Traumatology, Ninth People's Hospital of Chongqing, Chongqing, China.

Background: Complete discoid medial meniscus is an extremely rare abnormality of the knee joint whose meniscus has a discoid shape rather than a normal semilunar one. Several medial meniscus anomalies including anomalous insertion have been reported in the literature. This report presents a rare case of symptomatic complete discoid medial meniscus whose anterolateral (apical) portion was completely coalesced with the ACL. MRI, radiographic, and arthroscopic findings in the medial compartment are to be submitted.

Case Presentation: A 29-year-old male presented with intermittent pain and swelling of the right knee for 2 years. Based on radiographic, MRI, and physical examination findings, he was diagnosed with discoid medial meniscus tears. Arthroscopic saucerization was performed for the torn discoid medial meniscus of the right knee. Arthroscopic examination revealed a complete discoid medial meniscus and the anterolateral (apical) portion of which was completely coalesced with the ACL. Careful Probing of the meniscal surface revealed there was a longitudinal tear extending from the tibial spine to the midportion of the meniscus. Arthroscopic saucerization of the discoid meniscus was performed after closely cutting the meniscus around the ACL. The patient reported no symptoms, and he had returned to his daily and sports activities, including football, basketball, and jogging, at the 12-month follow-up.

Conclusion: Complete discoid medial meniscus is an extremely rare abnormality, and this case presents the third complete discoid medial meniscus whose anterolateral (apical) portion was completely coalesced with the ACL. The current case we present strongly supports the hypothesis that ACL and meniscus were differentiated from the same mesenchyme.
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http://dx.doi.org/10.1111/os.13377DOI Listing
August 2022

Temporal Dynamics of Subclinical Malaria in Different Transmission Zones of Myanmar.

Am J Trop Med Hyg 2022 Jul 25. Epub 2022 Jul 25.

University of Maryland School of Medicine, Baltimore, Maryland.

Countries in the Greater Mekong Subregion have committed to eliminate Plasmodium falciparum malaria by 2025. Subclinical malaria infections that can be detected by highly sensitive polymerase chain reaction (PCR) testing in asymptomatic individuals represent a potential impediment to this goal, although the extent to which these low-density infections contribute to transmission is unclear. To understand the temporal dynamics of subclinical malaria in this setting, a cohort of 2,705 participants from three epidemiologically distinct regions of Myanmar was screened for subclinical P. falciparum and P. vivax infection using ultrasensitive PCR (usPCR). Standard rapid diagnostic tests (RDTs) for P. falciparum were also performed. Individuals who tested positive for malaria by usPCR were followed for up to 12 weeks. Regression analysis was performed to estimate whether the baseline prevalence of infection and the count of repeated positive tests were associated with demographic, behavioral, and clinical factors. At enrollment, the prevalence of subclinical malaria infection measured by usPCR was 7.7% (1.5% P. falciparum monoinfection, 0.3% mixed P. falciparum and P. vivax, and 6.0% P. vivax monoinfection), while P. falciparum prevalence measured by RDT was just 0.2%. Prevalence varied by geography and was higher among older people and in those with outdoor exposure and travel. No difference was observed in either the prevalence or count of subclinical infection by time of year, indicating that even in low-endemicity areas, a reservoir of subclinical infection persists year-round. If low-density infections are shown to represent a significant source of transmission, identification of high-risk groups and locations may aid elimination efforts.
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http://dx.doi.org/10.4269/ajtmh.22-0027DOI Listing
July 2022

Tumor cell-released LC3-positive EVs promote lung metastasis of breast cancer through enhancing premetastatic niche formation.

Cancer Sci 2022 Jul 25. Epub 2022 Jul 25.

Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Immunology, Medical School of Southeast University, Nanjing, China.

Most breast cancer-related deaths are caused by metastasis in vital organs including the lungs. Development of supportive metastatic microenvironments, referred to as premetastatic niches (PMN), in certain distant organs before arrival of metastatic cells, is critical in metastasis. However, the mechanisms of PMN formation are not fully clear. Here, we demonstrated that chemoattractant C-C motif chemokine ligand 2 (CCL2) could be stimulated by heat shock protein 60 (HSP60) on the surface of murine 4T1 breast cancer cell-released LC3 extracellular vesicles (LC3 EVs) via the TLR2-MyD88-NF-κB signal cascade in lung fibroblasts, which subsequently promoted lung PMN formation through recruiting monocytes and suppressing T cell function. Consistently, reduction of LC3 EV release or HSP60 level, or neutralization of CCL2 markedly attenuated PMN formation and lung metastasis. Furthermore, the number of circulating LC3 EVs and HSP60 level on LC3 EVs in the plasma of breast cancer patients were positively correlated with disease progression and lung metastasis, which might have potential value as biomarkers of lung metastasis in breast cancer patients (AUC=0.898, 0.694, respectively). These findings illuminate a novel mechanism of PMN formation, and might provide therapeutic targets for anti-metastasis therapy for patients with breast cancer.
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http://dx.doi.org/10.1111/cas.15507DOI Listing
July 2022

SIRT1 deficiency increases O-GlcNAcylation of tau, mediating synaptic tauopathy.

Mol Psychiatry 2022 Jul 25. Epub 2022 Jul 25.

Department of Biochemistry and Molecular Biology, School of Medicine, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.

Hyperphosphorylation of the microtubule associated protein tau is associated with several neurodegenerative diseases including Alzheimer's Disease (AD), collectively referred to as tauopathies. However, the mechanisms by which tau is linked to synaptic dysfunction and memory impairment remain unclear. To address this question, we constructed a mouse model with brain-specific deficiency of SIRT1 (SIRT1 flox/Cre + ). Here, we show that increase of site-specific phosphorylation of tau is coupled with the strengthened O-GlcNAcylation of tau triggered by reduced O-GlcNAcase (OGA) and increased O-GlcNAc transferase (OGT) protein level in the brain of SIRT1 flox/Cre+ mice. SIRT1 deletion in mice brain changes the synaptosomal distribution of site-specific phospho-tau. Learning and memory deficiency induced by dendritic spine deficits and synaptic dysfunction are revealed via SIRT1 flox/Cre+ mice. Our results provide evidence for SIRT1 as a potential therapeutic target in clinical tauopathies.
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http://dx.doi.org/10.1038/s41380-022-01689-2DOI Listing
July 2022

Prospective surveillance of human adenovirus in acute respiratory infections reveals epidemiological features and the disappearance of species B during the COVID-19 pandemic in Beijing, China.

J Infect 2022 Jul 22. Epub 2022 Jul 22.

Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Institute for immunization and prevention, No.16 Hepingli Middle Street, Dongcheng, Beijing 100013, China; College of Public Health, Capital Medical University, Beijing 100069, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2022.07.015DOI Listing
July 2022

Development of Molecular Mechanisms and Their Application on Oncolytic Newcastle Disease Virus in Cancer Therapy.

Front Mol Biosci 2022 4;9:889403. Epub 2022 Jul 4.

Cancer Center, Department of Pathology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China.

Cancer is caused by the destruction or mutation of cellular genetic materials induced by environmental or genetic factors. It is defined by uncontrolled cell proliferation and abnormality of the apoptotic pathways. The majority of human malignancies are characterized by distant metastasis and dissemination. Currently, the most common means of cancer treatment include surgery, radiotherapy, and chemotherapy, which usually damage healthy cells and cause toxicity in patients. Targeted therapy is an effective tumor treatment method with few side effects. At present, some targeted therapeutic drugs have achieved encouraging results in clinical studies, but finding an effective solution to improve the targeting and delivery efficiency of these drugs remains a challenge. In recent years, oncolytic viruses (OVs) have been used to direct the tumor-targeted therapy or immunotherapy. Newcastle disease virus (NDV) is a solid oncolytic agent capable of directly killing tumor cells and increasing tumor antigen exposure. Simultaneously, NDV can trigger the proliferation of tumor-specific immune cells and thus improve the therapeutic efficacy of NDV in cancer. Based on NDV's inherent oncolytic activity and the stimulation of antitumor immune responses, the combination of NDV and other tumor therapy approaches can improve the antitumor efficacy while reducing drug toxicity, indicating a broad application potential. We discussed the biological properties of NDV, the antitumor molecular mechanisms of oncolytic NDV, and its application in the field of tumor therapy in this review. Furthermore, we presented new insights into the challenges that NDV will confront and suggestions for increasing NDV's therapeutic efficacy in cancer.
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http://dx.doi.org/10.3389/fmolb.2022.889403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289221PMC
July 2022

Mouse Models of Hepatocellular Carcinoma: Classification, Advancement, and Application.

Front Oncol 2022 30;12:902820. Epub 2022 Jun 30.

Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Hepatocellular carcinoma (HCC) is the subtype of liver cancer with the highest incidence, which is a heterogeneous malignancy with increasing incidence rate and high mortality. For ethical reasons, it is essential to validate medical clinical trials for HCC in animal models before further consideration on humans. Therefore, appropriate models for the study of the pathogenesis of the disease and related treatment methods are necessary. For tumor research, mouse models are the most commonly used and effective model, which is closer to the real-life environment, and the repeated experiments performed on it are closer to the real situation. Several mouse models of HCC have been developed with different mouse strains, cell lines, tumor sites, and tumor formation methods. In this review, we mainly introduce some mouse HCC models, including induced model, gene-edited model, HCC transplantation model, and other mouse HCC models, and discuss how to choose the appropriate model according to the purpose of the experiments.
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http://dx.doi.org/10.3389/fonc.2022.902820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279915PMC
June 2022

Application of metagenomic next-generation sequencing for the diagnosis of intracranial infection of .

Ann Transl Med 2022 Jun;10(12):672

Department of Intensive Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Intracranial infection of (LM) can lead to various manifestations, including meningitis, meningoencephalitis, brainstem encephalitis, and brain abscess, which often have a poor prognosis. Metagenomic next-generation sequencing (mNGS) is a promising new tool for the diagnosis of intracranial infection of LM. We describe the typical clinical manifestations of LM intracranial infection and highlight its rarity and severity to help physicians better understand the disease characteristics.

Methods: Six cases of severe LM intracranial infection were diagnosed by mNGS. We conducted a retrospective analysis of the data on disease progression, diagnostic tools, treatments, and outcomes, and summarized the findings. We compared the differences in diagnostic accuracy and timeliness between mNGS and etiological cultures.

Results: Among the 6 patients, 5 were males and 1 was female (age range 32-83). Three patients had a history of immunosuppressive therapy. Common symptoms included fever (100%) and a stiff neck (100%). Coma occurred early in severe patients (66%). Two healthy young patients had previously developed with meningitis, while coma occurred in 3 immunosuppressed patients and 1 elderly patient. Three immunosuppressed patients presented with brain abscess, brainstem encephalitis, and meningitis. 1 elderly patient presented with meningitis. Two patients developed septic shock complications early. Laboratory data showed normal or slightly increased leukocytes, neutrophils, and procalcitonin, and cerebrospinal fluid (CSF) tests were consistent with bacterial CSF infection. All 6 patients were examined for blood culture and CSF culture. The positive rate of blood culture and CSF culture was 50% and 16%. The average time from admission to positive culture findings was 91 h. All 6 patients were examined for CSF mNGS. Two were also examined for whole-blood mNGS. The positive rate for CSF mNGS and whole-blood mNGS results was 100%. The mean time from admission to positive mNGS report was 47 h. After diagnosis and treatment with sensitive antibiotics, 1 patient with brain abscess developed neurological sequelae, while the other 5 patients completely recovered.

Conclusions: mNGS can improve accuracy in the diagnosis of LM intracranial infection and reduce the delay in diagnosis. Intracranial infection of Listeria monocytogenes responds well to the timely use of appropriate antibiotics.
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http://dx.doi.org/10.21037/atm-22-2186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279819PMC
June 2022

Development of a novel and fast XRF instrument for large area heavy metal detection integrated with UAV.

Environ Res 2022 Jul 14;214(Pt 2):113841. Epub 2022 Jul 14.

Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, PR China. Electronic address:

The disadvantages of the current chemical and instrumental analysis methods for soil heavy metal pollution are that they have a high detection cost, long cycle times, and may cause secondary pollution. The aims of this study were to improve the rapid detection of soil heavy metal pollution over large areas. This study combined aircraft technology, embedded development, computer software, electronic information, and other technical methods to create a novel solution to the problem, i.e., an integrated unmanned aerial vehicle (UAV) based soil heavy metal pollution rapid detection system (UAV-SHMPRDS) was built. The key technologies required for a rapid detection system were developed, including the development of a hardware system based on a UAV and an X-ray fluorescence spectrum (XRF) analyzer, the design and implementation of a control system software system, and the implementation of a data inversion processing algorithm. Finally, a prototype UAV-SHMPRDS was constructed. Testing showed that the system improved regionalized soil heavy metal pollution detection efficiency. This study provides new solutions for the current problems encountered in the actual rapid detection of soil heavy metal pollution.
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http://dx.doi.org/10.1016/j.envres.2022.113841DOI Listing
July 2022

HDL-C levels added to the MELD score improves 30-day mortality prediction in Asian patients with cirrhosis.

J Int Med Res 2022 Jul;50(7):3000605221109385

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objectives: Lower high-density lipoprotein cholesterol (HDL-C) levels have been observed in chronic liver disease patients. The aim of this study was to develop a model that incorporates HDL-C levels and the Model for End-stage Liver Disease (MELD) score to predict 30-day mortality in Asian cirrhosis patients.

Methods: Cirrhosis patients were recruited from two hospitals in this retrospective observational study. Propensity score matching was used. The model's performance was evaluated, including its ability to predict 30-day mortality, accuracy, and clinical utility. Net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were calculated.

Results: The HDL-C + MELD model showed good ability to predict 30-day mortality (area under the curve, 0.784; sensitivity, 0.797; specificity, 0.632), which was better than that of the MELD score alone. It also showed good calibration and a net benefit for all patients, which was better than that of the MELD score, except at the threshold probability. NRI and IDI results confirmed that adding HDL-C levels to the MELD score improved the model's performance in predicting 30-day mortality.

Conclusion: We added HDL-C levels to the MELD score to predict 30-day mortality in Asian patients with cirrhosis. The HDLC + MELD model shows good ability to predict 30-day mortality and clinical utility.
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http://dx.doi.org/10.1177/03000605221109385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9290124PMC
July 2022

Integration of metabolomics and transcriptomics to reveal anti-chronic myocardial ischemia mechanism of Gualou Xiebai decoction.

J Ethnopharmacol 2022 Oct 10;297:115530. Epub 2022 Jul 10.

College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China. Electronic address:

Ethnopharmacological Relevance: Gualou Xiebai decoction (GLXB), a well-known classic traditional Chinese medicine formula, is a recorded and proven therapy for the management of cardiac diseases. However, its pharmacological characteristics and mechanism of action are unclear.

Materials And Methods: The effects of GLXB and its mechanism of action in an isoprenaline-induced rat model of chronic myocardial ischemia (CMI) were investigated by incorporating metabonomics and transcriptomics. Meanwhile, the echocardiographic evaluation, histopathological analysis, serum biochemistry assay, TUNEL assay and western blot analysis were detected to revealed the protective effects of GLXB on CMI.

Results: The results of echocardiographic evaluation, histopathological analysis and serum biochemistry assay revealed that GLXB had a significantly cardioprotective performance by reversing echocardiographic abnormalities, restoring pathological disorders and converting the serum biochemistry perturbations. Further, the omics analysis indicated that many genes and metabolites were regulated after modeling and GLXB administration, and maintained the marked "high-low" or "low-high" trends. Meanwhile, the results from integrated bioinformatics analysis suggested that the interaction network mainly consisted of amino acid and organic acid metabolism. The results of TUNEL assay and western blot analysis complemented the findings of integrated analysis of metabolomics and transcriptomics.

Conclusion: These findings suggested that GLXB has a curative effect in isoproterenol-induced CMI in rats. Integrated analysis based on transcriptomics and metabolomics studies revealed that the mechanism of GLXB in alleviating CMI was principally by the regulation of energy homeostasis and apoptosis, which was through a multi-component and multi-target treatment modality.
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http://dx.doi.org/10.1016/j.jep.2022.115530DOI Listing
October 2022

Identification of Nordic Berries with Beneficial Effects on Cognitive Outcomes and Gut Microbiota in High-Fat-Fed Middle-Aged C57BL/6J Mice.

Nutrients 2022 Jun 30;14(13). Epub 2022 Jun 30.

Berry Lab AB, Scheelevägen 22, 223 63 Lund, Sweden.

High-fat diets are associated with neuronal and memory dysfunction. Berries may be useful in improving age-related memory deficits in humans, as well as in mice receiving high-fat diets. Emerging research has also demonstrated that brain health and cognitive function may be related to the dynamic changes in the gut microbiota. In this study, the impact of Nordic berries on the brain and the gut microbiota was investigated in middle-aged C57BL/6J mice. The mice were fed high-fat diets (60%E fat) supplemented with freeze-dried powder (6% dwb) of bilberry, lingonberry, cloudberry, blueberry, blackcurrant, and sea buckthorn for 4 months. The results suggest that supplementation with bilberry, blackcurrant, blueberry, lingonberry, and (to some extent) cloudberry has beneficial effects on spatial cognition, as seen by the enhanced performance following the T-maze alternation test, as well as a greater proportion of DCX-expressing cells with prolongation in hippocampus. Furthermore, the proportion of the mucosa-associated symbiotic bacteria increased by 4-14 times in the cecal microbiota of mice fed diets supplemented with lingonberry, bilberry, sea buckthorn, and blueberry. These findings demonstrate the potential of Nordic berries to preserve memory and cognitive function, and to induce alterations of the gut microbiota composition.
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http://dx.doi.org/10.3390/nu14132734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269296PMC
June 2022

Fitness Promotion in a Jump Rope-Based Homework Intervention for Middle School Students: A Randomized Controlled Trial.

Front Psychol 2022 16;13:912635. Epub 2022 Jun 16.

School of Physical Education, Qingdao University, Qingdao, China.

Physical activity (PA) homework offers a promising approach for students to be physically active after school. The current study aims to provide holistic insights into PA homework design and the effects of implementation in practice. In total, ninety-three middle school students were randomly assigned to a homework group (HG) or control group (CG). Participants in HG ( = 47) were requested to complete jump rope homework three times per week for 12 weeks, while their counterparts in CG attended one health education class every week. A homework sheet was used to provide instructions and record information for exercise behaviors during homework completion. Physical fitness tests were conducted to investigate the effects of the jump rope homework on the physical fitness of middle school students. After the intervention, participants in HG reported moderate to vigorous PA during jump rope exercise. The average duration for each practice was approximately 48 min. The returned homework sheets accounted for 86.88% of all homework assignments, indicating a good completion rate. Compared with their counterparts in CG, participants performing jump rope exercise indicated greater improvement in speed, endurance, power, and core muscular endurance. Jump rope homework strengthened physical fitness for middle school students, which provided a valuable addition to comprehensive school PA practice.
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http://dx.doi.org/10.3389/fpsyg.2022.912635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244795PMC
June 2022

Correction: The hangman effect boosts hydrogen production by a manganese terpyridine complex.

Chem Commun (Camb) 2022 Jul 5;58(54):7579. Epub 2022 Jul 5.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, P. R. China.

Correction for 'The hangman effect boosts hydrogen production by a manganese terpyridine complex' by Qianqian Wu , , 2022, , 5128-5131, https://doi.org/10.1039/D2CC00757F.
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http://dx.doi.org/10.1039/d2cc90224aDOI Listing
July 2022

Single-Atom Ce-Modified α-FeO for Selective Catalytic Reduction of NO with NH.

Environ Sci Technol 2022 Jul 24;56(14):10442-10453. Epub 2022 Jun 24.

Jiangsu Key Laboratory of Vehicle Emissions Control, School of the Environment, Nanjing University, Nanjing 210093, P. R. China.

A single-atom Ce-modified α-FeO catalyst (FeCeO catalyst with 7% atomic percentage of Ce) was synthesized by a citric acid-assisted sol-gel method, which exhibited excellent performance for selective catalytic reduction of NO with NH (NH-SCR) over a wide operating temperature window. Remarkably, it maintained ∼93% NO conversion efficiency for 168 h in the presence of 200 ppm SO and 5 vol % HO at 250 °C. The structural characterizations suggested that the introduction of Ce leads to the generation of local Fe-O-Ce sites in the FeO matrix. Furthermore, it is critical to maintain the atomic dispersion of the Ce species to maximize the amounts of Fe-O-Ce sites in the Ce-doped FeO catalyst. The formation of CeO nanoparticles due to a high doping amount of Ce species leads to a decline in catalytic performance, indicating a size-dependent catalytic behavior. Density functional theory (DFT) calculation results indicate that the formation of oxygen vacancies in the Fe-O-Ce sites is more favorable than that in the Fe-O-Fe sites in the Ce-free α-FeO catalyst. The Fe-O-Ce sites can promote the oxidation of NO to NO on the FeCeO catalyst and further facilitate the reduction of NO by NH. In addition, the decomposition of NHHSO can occur at lower temperatures on the FeCeO catalyst containing atomically dispersed Ce species than on the α-FeO reference catalyst, resulting in the good SO/HO resistance ability in the NH-SCR reaction.
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http://dx.doi.org/10.1021/acs.est.2c02916DOI Listing
July 2022

Population Pharmacokinetics of Tigecycline: A Systematic Review.

Drug Des Devel Ther 2022 17;16:1885-1896. Epub 2022 Jun 17.

Department of Emergency Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, People's Republic of China.

Although tigecycline is widely used in clinical practice, its efficiency and optimal dosage regimens remain controversial. The purpose of this article was to help guide tigecycline dosing in different patient subpopulations through comparing the published population pharmacokinetic models of tigecycline, as well as summarizing and determining the potential covariates that markedly influence tigecycline pharmacokinetics. In this review, literature was systematically searched from the PubMed database from inception to March 2022. The articles focusing on population pharmacokinetics for tigecycline in healthy volunteers or patients were included; finally, a total of eight studies were included in this review. NONMEM methods were used in five studies to generate the population pharmacokinetic models. Tigecycline pharmacokinetics were mostly described by a two-compartment model in these included studies. Estimated clearance and volumes of distribution of tigecycline at steady state () varied widely in different target patient populations, with a range of 7.5-23.1 L/h and 212.7-1087.7 L, respectively. Body-weight and creatinine clearance were the most important predictors of clearance in these studies, while other predictors include age, gender, bilirubin and aspartate aminotransferase. In conclusion, this review showed the large variability of tigecycline population pharmacokinetics, which can provide guide dosing in different target populations. For clinicians, the individual dosing adjustment should be based not only on the indication and pathogen susceptibility but also on the potential important predictors. However, more studies were needed to confirm the necessity of modified dosage regimens in different patient subpopulations.
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http://dx.doi.org/10.2147/DDDT.S365512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211078PMC
June 2022

Calorie restriction on normal body weight mice prevents body weight regain on a follow-up high-fat diet by shaping an obesity-resistant-like gut microbiota profile.

Food Funct 2022 Jul 18;13(14):7684-7696. Epub 2022 Jul 18.

Department of Nutrition and Food Safety, School of Public Health, Fujian Medical University, FuZhou 350122, P.R. China.

Calorie restriction (CR) is one of the most common approaches for obesity treatment, but whether resuming feeding after CR in normal-weight mice can affect excessive weight regain remains poorly studied. To address this issue, male C57BL/6 mice were placed in three groups: a control group ( = 10), a group fed normal diet with 30% CR ( = 20); and a group fed a HF diet ( = 30). After four weeks, the CR group was fed either a normal diet (NDCR, = 10) or a high-fat diet (HFCR, = 10) for an additional eight weeks. At the end of the experiment, mice in the HF group ranked in the upper and lower thirds for weight gain were designated as obesity-prone (HFOP, = 10) and obesity-resistant (HFOR, = 10), respectively. CR delayed weight regain and visceral fat accumulation. Gut microbiota in the HFCR group were more similar to the HFOR group than the HFOP group, mainly due to reversion of the decreased level of Clostridiales induced by CR. Mediation analysis showed that Clostridiales may delay body weight regain by affecting the interconversion of succinate and fumarate. Random forest and structural equation analyses showed Christensenellaceae were the most important biomarker for alleviation of obesity. In conclusion, CR shapes an obesity-resistant-like gut microbiota profile that may attenuate body weight regain.
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http://dx.doi.org/10.1039/d1fo04358gDOI Listing
July 2022

Genome sequence of Gossypium anomalum facilitates interspecific introgression breeding.

Plant Commun 2022 Jun 22:100350. Epub 2022 Jun 22.

Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs, the Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Electronic address:

Crop wild relatives are an important reservoir of natural biodiversity. However, incorporating wild genetic diversity into breeding programs is often hampered by reproductive barriers and a lack of accurate genomic information. We assembled a high-quality, accurately centromere-anchored genome of Gossypium anomalum, a stress-tolerant wild cotton species. We provided a strategy to discover and transfer agronomically valuable genes from wild diploid species to tetraploid cotton cultivars. With a (Gossypium hirsutum × G. anomalum) hexaploid as a bridge parent, we developed a set of 74 diploid chromosome segment substitution lines (CSSLs) of the wild cotton species G. anomalum in the G. hirsutum background. This set of CSSLs included 70 homozygous substitutions and four heterozygous substitutions, and it collectively contained about 72.22% of the G. anomalum genome. Twenty-four quantitative trait loci associated with plant height, yield, and fiber qualities were detected on 15 substitution segments. Integrating the reference genome with agronomic trait evaluation of the CSSLs enabled location and cloning of two G. anomalum genes that encode peroxiredoxin and putative callose synthase 8, respectively, conferring drought tolerance and improving fiber strength. We have demonstrated the power of a high-quality wild-species reference genome for identifying agronomically valuable alleles to facilitate interspecific introgression breeding in crops.
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http://dx.doi.org/10.1016/j.xplc.2022.100350DOI Listing
June 2022

Transcriptional Alterations of Mouse Trigeminal Ganglion Neurons Following Orofacial Inflammation Revealed by Single-Cell Analysis.

Front Cell Neurosci 2022 2;16:885569. Epub 2022 Jun 2.

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.

Orofacial inflammation leads to transcriptional alterations in trigeminal ganglion (TG) neurons. However, diverse alterations and regulatory mechanisms following orofacial inflammatory pain in different types of TG neurons remain unclear. Here, orofacial inflammation was induced by injection of complete Freund's adjuvant (CFA) in mice. After 7 days, we performed single-cell RNA-sequencing on TG cells of mice from control and treatment groups. We identified primary sensory neurons, Schwann cells, satellite glial cells, oligodendrocyte-like cells, immune cells, fibroblasts, and endothelial cells in TG tissue. After principal component analysis and hierarchical clustering, we identified six TG neuronal subpopulations: peptidergic nociceptors (PEP1 and PEP2), non-peptidergic nociceptors (NP1 and NP2), C-fiber low-threshold mechanoreceptors (cLTMR) and myelinated neurons (-positive neurons, NF) based on annotated marker gene expression. We also performed differential gene expression analysis among TG neuronal subtypes, identifying several differential genes involved in the inflammatory response, neuronal excitability, neuroprotection, and metabolic processes. Notably, we identified several potential novel targets associated with pain modulation, including , , , and in PEP1, in PEP2, and in cLTMR. The established protein-protein interaction network identified some hub genes, implying their critical involvement in regulating orofacial inflammatory pain. Our study revealed the heterogeneity of TG neurons and their diverse neuronal transcriptomic responses to orofacial inflammation, providing a basis for the development of therapeutic strategies for orofacial inflammatory pain.
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http://dx.doi.org/10.3389/fncel.2022.885569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200971PMC
June 2022

The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control.

Plant Cell 2022 Jun 16. Epub 2022 Jun 16.

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

Photosynthesis and the biosynthesis of many important metabolites occur in chloroplasts. In these semi-autonomous organelles, the chloroplast genome encodes approximately 100 proteins. The remaining chloroplast proteins, close to 3000, are encoded by nuclear genes whose products are translated in the cytosol and imported into chloroplasts. However, there is still no consensus on the composition of the protein import machinery including its motor proteins and on how newly imported chloroplast proteins are refolded. In this study, we have examined the function of orf2971, the largest chloroplast gene of Chlamydomonas reinhardtii. Depletion of Orf2971 causes accumulation of protein precursors, partial proteolysis and aggregation of proteins, increased expression of chaperones and proteases, and autophagy. Orf2971 interacts with the TIC complex, catalyzes ATP hydrolysis and associates with chaperones and chaperonins. We propose that Orf2971 is intimately connected to the protein import machinery and plays an important role in chloroplast protein quality control.
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http://dx.doi.org/10.1093/plcell/koac180DOI Listing
June 2022

A preliminary study on the relationship between environmental endocrine disruptors and precocious puberty in girls.

J Pediatr Endocrinol Metab 2022 Aug 14;35(8):989-997. Epub 2022 Jun 14.

Traditional Chinese Medical Hospital of Zhuji, Zhuji, P.R. China.

Objectives: To explore the associations of environmental endocrine disruptors on precocious puberty in girls.

Methods: This was a case-control study in which 30 girls with precocious puberty and 46 age- and race-matched prepubertal females were enrolled. The concentrations of 10 environment endocrine disruptors (bisphenol A, bisphenol B, butylparaben, propylparaben, ethvlparaben, methylparaben, mono-butyl phthalate, mono-2-ethylhexyl phthalate, monoethyl phthalate, and monomethyl phthalate) in urine and 10 steroid hormones (dihydrotestosterone, corticosterone, hydrocortisone, 11-deoxycortisol, 17α-hydroxy progesterone, 4-androstene-3,17-dione, estrone, deoxycorticosterone, pregnenolone, and dehydroepiandrosterone) in serum were detected with the liquid chromatography-mass spectrometry (LC-MS).

Results: According to the Mann-Whitney U test, urinary levels of bisphenol A, monobutyl phthalate, and monomethyl phthalate were significantly higher in the precocious group than in the prepubertal group, and blood levels of hydrocortisone, 11-deoxycortisol, corticosterone, deoxycorticosterone, and pregnenolone were significantly lower in the precocious group than in the prepubertal group (p<0.05, VIP>1).

Conclusions: Our findings confirm the association between phthalate exposure and the incidence of precocious puberty in girls. Control and reduction of children exposure to phthalate esters should be considered as a health priority.
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http://dx.doi.org/10.1515/jpem-2021-0691DOI Listing
August 2022

Lignin-containing hydrogels with anti-freezing, excellent water retention and super-flexibility for sensor and supercapacitor applications.

Int J Biol Macromol 2022 Aug 9;214:77-90. Epub 2022 Jun 9.

College of Material Engineering, Fujian Agriculture and Forestry University, Fuzhou 350108, China; Department of Chemical Engineering, University of New Brunswick, Fredericton E3B 5A3, Canada. Electronic address:

We developed a highly conductive and flexible, anti-freezing sulfonated lignin (SL)-containing polyacrylic acid (PAA) (SL-g-PAA-Ni) hydrogel, with a high concentration of NiCl. Ni contributes multi-functions to the preparation of the hydrogel and its final properties, such as fast polymerization reaction as a result of the presence of redox pairs of Ni/Ni and hydroquinone/quinone, and anti-freezing properties of the hydrogel due to the salt effects of NiCl so that at -20 °C the hydrogel shows similar properties to those at the room temperature. Thanks to the effective coordinations of Ni with catecholic groups and carboxylic groups, as well as the rich hydrogen bonding capacity, the resultant hydrogel possesses excellent mechanical properties. High ionic conductivity (6.85 S·m) of the hydrogel is obtained due to the supply of high concentration of Ni. Moreover, the ionic solvation effect of NiCl in the hydrogel imparts excellent water retention ability, with water retention of ~93 % after 21-day storage. The SL-g-PAA-Ni hydrogel can accurately detect various human motions at -20 °C. The supercapacitor assembled from SL-g-PAA-Al hydrogel at -20 °C manifests a high specific capacitance of 252 F·g, with maximum energy density of 26.97 Wh·kg, power density of 2667 W·kg, and capacitance retention of 96.7 % after 3000 consecutive charge-discharge cycles.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.030DOI Listing
August 2022

Mechanism of Shiliu Buxue Syrup for anemia using integrated metabolomics and network pharmacology.

Anal Biochem 2022 09 8;653:114774. Epub 2022 Jun 8.

College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, China; Xinjiang Uygur Pharmaceutical Co., Ltd., Urumqi, Xinjiang, 830026, China. Electronic address:

For many years, Shiliu Buxue Syrup (SLBXS) has been used in the treatment of anemia in Xinjiang, China. However, the potential therapeutic mechanism of SLBXS in the treatment of anemia remains unclear. We qualitatively analyzed the ingredients of SLBXS and predicted the underlying mechanisms by network pharmacology. A mice model of anemia was established by subcutaneous injection of 1-Acetyl-2-phenylhydrazine (APH). Spleen metabolomics was performed to screen potential biomarkers and pathways related to anemia. Furthermore, core targets of crucial pathways were experimentally validated. Finally, molecular docking was used for predicting interactions between compositions and targets. Network pharmacology indicated that the 230 SLBXS ingredients may affect 141 target proteins to regulate the PI3K/AKT and HIF-1 signaling pathways. Metabolomics revealed that SLBXS could mediate 30 biomarkers, such as phosphoric acid, l-pyroglutamic acid, alpha-Tocopherol, 1-stearoyl-rac-glycerol, and dihydroxyacetone phosphate, to regulate drug metabolism-other enzymes, glutathione metabolism, glycolysis or gluconeogenesis, nicotinate and nicotinamide metabolism, nitrogen metabolism, and purine metabolism. Western blot indicated that SLBXS can regulate the protein expression levels of AKT1, Bcl2, Caspase3, HIF-1α, VEGF-A, and NOS2. The molecular docking revealed that most of the compositions had a good binding ability to the core targets. Based on these findings, we speculate that SLBXS treats anemia mainly by modulating the PI3K/AKT and HIF-1 pathways and glutathione and glycolytic metabolisms.
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http://dx.doi.org/10.1016/j.ab.2022.114774DOI Listing
September 2022

DCAF12 and HSPA1A May Serve as Potential Diagnostic Biomarkers for Myasthenia Gravis.

Biomed Res Int 2022 24;2022:8587273. Epub 2022 May 24.

Department of Neurology, First Affiliated Hospital, Guangxi Medical University, Nanning, China 530021.

Background: Myasthenia gravis (MG) is an autoimmune disease that severely affects the life quality of patients. This study explores the differences in immune cell types between MG and healthy control and the role of immune-related genes in the diagnosis of MG.

Methods: The GSE85452 dataset was downloaded from the Gene Expression Omnibus (GEO) database and analyzed using the limma package to determine differentially expressed genes (DEGs) between patients with MG and the control group. Differentially expressed immune cells were analyzed using single-sample gene set enrichment analysis (GSEA), while immune cell-associated modules were identified by weighted gene coexpression network analysis (WGCNA). Then, the expression of the identified hub genes was confirmed by RT-PCR in peripheral blood mononuclear cells (PBMCs) of MG patients. The R package pROC was used to plot the receiver operating characteristics (ROC) curves.

Results: The modules related to CD56 natural killer cells were identified by GSEA and WGCNA. The proportion of CD56 natural killer cells in the peripheral blood of MG patients is low. The results of RT-PCR showed that the levels of DDB1- and CUL4-associated factor 12 (DCAF12) and heat shock protein family A member 1A (HSPA1A) were significantly decreased in peripheral blood mononuclear cells of MG patients compared with healthy controls. The ROC curve results of DCAF12 and HSPA1A mRNA in MG diagnosis were 0.780 and 0.830, respectively.

Conclusions: CD56 NK cell is lower in MG patients and may affect MG occurrence. DCAF12 and HSPA1A are lowly expressed in PBMCs of MG patients and may serve as the diagnostic biomarkers of MG.
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http://dx.doi.org/10.1155/2022/8587273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9155969PMC
June 2022
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