Publications by authors named "Fang Guo"

385 Publications

The anti-tumor effects of the combination of microwave hyperthermia and lobaplatin against breast cancer cells in vitro and in vivo.

Biosci Rep 2021 Jul 20. Epub 2021 Jul 20.

Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Background:  Breast cancer is the main lethal disease among females. The combination of lobaplatin and microwave hyperthermia plays a crucial role in several kinds of cancer in the clinic, but it's possible mechanism in breast cancer has remained indistinct. Methods: Mouse models were used to detect breast cancer progression. Cell growth was explored with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulphonyl)-2H-tetrazolium) and colony formation assays. Cell migration and invasion were investigated with a transwell assay. Cell apoptosis was probed with flow cytometry. The expression of apoptosis-associated proteins was examined with Western blots. Result: Combination treatment decreased breast cancer cell viability, colony formation, cell invasion and metastasis. In addition, the treatment induced breast cancer cell apoptosis and autophagy, activated the JNK signaling pathway, suppressed the AKT/mTOR signaling pathway, and downregulated IAP and Bcl-2 family protein expression. Conclusion: These results indicate that lobaplatin is an effective breast cancer antitumor agent. Microwave hyperthermia was a useful adjunctive treatment. Combination treatment was more efficient than any single therapy. The possible mechanism for this effect was mainly associated with activation of the JNK signaling pathway, inactivation of the AKT/mTOR signaling pathway and down regulation of the Bcl-2 and IAP families. Key words: lobaplatin, microwave hyperthermia, apoptosis, autophagy, mTOR, JNK.
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http://dx.doi.org/10.1042/BSR20190878DOI Listing
July 2021

[Retracted] miR‑22 inhibits proliferation and invasion in estrogen receptor α‑positive endometrial endometrioid carcinomas cells.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xinxiang Medical University,Weihui, Henan 453100, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the cell‑cycle data shown in Fig. 4A and Transwell cell migration data shown in Fig. 5A were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 9: 2393‑2399, 2014; DOI: 10.3892/mmr.2014.2123].
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http://dx.doi.org/10.3892/mmr.2021.12280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281490PMC
September 2021

Senescent Changes and Endoplasmic Reticulum Stress May Be Involved in the Pathogenesis of Missed Miscarriage.

Front Cell Dev Biol 2021 17;9:656549. Epub 2021 Jun 17.

Department of Obstetrics and Gynaecology, The University of Auckland, Auckland, New Zealand.

Background: Senescence is involved in many complications of pregnancy. However, whether senescent changes are also associated with missed miscarriage has not been fully investigated.

Methods: The levels of p16, p21, and γH2AX, markers of senescence, were measured in placentas collected from women with missed miscarriage by immunohistochemistry and Western blotting. Levels of misfolded proteins in missed miscarriage placentas or normal first-trimester placenta that had been treated with HO (100 μM) or extracellular vesicles (EVs) collected from missed miscarriage placental explant culture were measured by fluorescent compound, thioflavin-T. The production of reactive oxygen species (ROS) by missed miscarriage placentas was measured by CellROX Deep Red.

Results: Increased levels of p16, p21, and γH2AX were presented in missed miscarriage placentas compared to controls. Increased levels of misfolded proteins were shown in missed miscarriage placentas, but not in EVs that were collected from missed miscarriage placentas. The ROS production was significantly increased in missed miscarriage placental explant cultures. Increased levels of misfolded proteins were seen in the normal first-trimester placenta that had been treated with HO compared to untreated.

Conclusion: Our data demonstrate that there are increases in senescence and endoplasmic reticulum stress and ROS production in missed miscarriage placenta. Oxidative stress and an accumulation of misfolded proteins in missed miscarriage placentas may contribute to the changes of senescence and endoplasmic reticulum stress seen in missed miscarriage placentas.
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http://dx.doi.org/10.3389/fcell.2021.656549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247570PMC
June 2021

A Comparison of Treatment Options for Type 1 and Type 2 Caesarean Scar Pregnancy: A Retrospective Case Series Study.

Front Med (Lausanne) 2021 15;8:671035. Epub 2021 Jun 15.

Department of Obstetrics and Gynaecology, The University of Auckland, Auckland, New Zealand.

There is currently no agreement on the optimal management of caesarean scar pregnancy. Caesarean scar pregnancy is currently categorised into two subtypes according to the site of implantation. This may consequently result in the difference in treatment options. However, the comparison of the success rate of each treatment option according to the subtypes has not been fully investigated. 71 patients who were treated by uterine curettage (D and C), or uterine artery embolization with curettage (UAE) or hysteroscopy in conjunction with laparoscopy between January 2016 and March 2020 were included. Data on maternal age, gestational sac age, the sac diameter, the interval between two pregnancies, the number of previous caesarean sections, amount of bleeding and β-hCG levels were collected and analysed dependent on the subtypes. There was no difference in the clinical parameters of the cases who received different options of treatment, as well as no difference in the clinical parameters between type 1 and type 2 caesarean scar pregnancy. The primary success rate for type 1 caesarean scar pregnancy by D and C, or UAE, or hysteroscopy in conjunction with laparoscopy was 95, or 100 or 100%, respectively. The primary success rate for type 2 caesarean scar pregnancy by D and C, or UAE, or hysteroscopy in conjunction with laparoscopy was 27, or 67, or 95% respectively. Our data demonstrates that hysteroscopy in conjunction with laparoscopy for type 2 caesarean scar pregnancy was the most successful compared to other options, but for type 1 caesarean scar pregnancy, D and C could be the cost-effective option.
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http://dx.doi.org/10.3389/fmed.2021.671035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240870PMC
June 2021

177 Saponins, Including 11 New Compounds in Wild Ginseng Tentatively Identified via HPLC-IT-TOF-MS, and Differences among Wild Ginseng, Ginseng under Forest, and Cultivated Ginseng.

Molecules 2021 Jun 2;26(11). Epub 2021 Jun 2.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.

Wild ginseng (W-GS), ginseng under forest (F-GS, planted in mountain forest and growing in natural environment), and cultivated ginseng (C-GS) were compared via HPLC-DAD and HPLC-IT-TOF-MS. A total of 199 saponins, including 16 potential new compounds, were tentatively identified from 100 mg W-GS (177 saponins in W-GS with 11 new compounds), F-GS (56 saponins with 1 new compound), and C-GS (60 saponins with 6 new compounds). There were 21 saponins detected from all the W-GS, F-GS, and C-GS. Fifty saponins were only detected from W-GS, including 23 saponins found in ginseng for the first time. Contents of ginsenosides Re (12.36-13.91 mg/g), Rh (7.46-7.65 mg/g), Rd (12.94-12.98 mg/g), and the total contents (50.52-55.51 mg/g) of Rg, Re, Rf, Rb, Rg, Rh, and Rd in W-GS were remarkably higher than those in F-GS (Re 1.22-3.50 mg/g, Rh 0.15-1.49 mg/g, Rd 0.19-1.49 mg/g, total 5.69-18.74 mg/g), and C-GS (Re 0.30-3.45 mg/g, Rh 0.05-3.42 mg/g, Rd 0.17-1.68 mg/g, total 2.99-19.55 mg/g). Contents of Re and Rf were significantly higher in F-GS than those in C-GS ( < 0.05). Using the contents of Re, Rf, or Rb, approximately a half number of cultivated ginseng samples could be identified from ginseng under forest. Contents of Rg, Re, Rg, Rh, as well as the total contents of the seven ginsenosides were highest in ginseng older than 15 years, middle-high in ginseng between 10 to 15 years old, and lowest in ginseng younger than 10 years. Contents of Rg, Re, Rf, Rb, Rg, and the total of seven ginsenosides were significantly related to the growing ages of ginseng ( < 0.10). Similarities of chromatographic fingerprints to W-GS were significantly higher ( < 0.05) for F-GS (median: 0.824) than C-GS (median: 0.745). A characteristic peak pattern in fingerprint was also discovered for distinguishing three types of ginseng. Conclusively, wild ginseng was remarkably superior to ginseng under forest and cultivated ginseng, with ginseng under forest slightly closer to wild ginseng than cultivated ginseng. The differences among wild ginseng, ginseng under forest, and cultivated ginseng in saponin compositions and contents of ginsenosides were mainly attributed to their growing ages.
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http://dx.doi.org/10.3390/molecules26113371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199768PMC
June 2021

Host poly(A) polymerases PAPD5 and PAPD7 provide two layers of protection that ensure the integrity and stability of hepatitis B virus RNA.

J Virol 2021 Jun 30:JVI0057421. Epub 2021 Jun 30.

Arbutus Biopharma, Warminster, Pennsylvania, USA.

Noncanonical poly(A) polymerases PAPD5 and PAPD7 (PAPD5/7) stabilize HBV RNA via the interaction with the viral post-transcriptional regulatory element (PRE), representing new antiviral targets to control HBV RNA metabolism, HBsAg production and viral replication. Inhibitors targeting these proteins are being developed as antiviral therapies, therefore it is important to understand how PAPD5/7 coordinate to stabilize HBV RNA. Here, we utilized a potent small-molecule AB-452 as a chemical probe, along with genetic analyses to dissect the individual roles of PAPD5/7 in HBV RNA stability. AB-452 inhibits PAPD5/7 enzymatic activities and reduces HBsAg both (EC ranged from 1.4 to 6.8 nM) and by 0.93 log. Our genetic studies demonstrate that the stem-loop alpha sequence within PRE is essential for both maintaining HBV poly(A) tail integrity and determining sensitivity towards the inhibitory effect of AB-452. Although neither single knock-out (KO) of nor reduces HBsAg RNA and protein production, KO does impair poly(A) tail integrity and confers partial resistance to AB-452. In contrast, KO did not result in any measurable changes within the HBV poly(A) tails, but cells with both and KO show reduced HBsAg production and conferred complete resistance to AB-452 treatment. Our results indicate that PAPD5 plays a dominant role in stabilizing viral RNA by protecting the integrity of its poly(A) tail, while PAPD7 serves as a second line of protection. These findings inform PAPD5 targeted therapeutic strategies and open avenues for further investigating PAPD5/7 in HBV replication. Chronic hepatitis B affects more than 250 million patients and is a major public health concern worldwide. HBsAg plays a central role in maintaining HBV persistence and as such, therapies that aim at reducing HBsAg through destabilizing or degrading HBV RNA have been extensively investigated. Besides directly degrading HBV transcripts through antisense oligonucleotides or RNA silencing technologies, small-molecule compounds targeting host factors such as the noncanonical poly(A) polymerase PAPD5 and PAPD7 have been reported to interfere with HBV RNA metabolism. Herein, our antiviral and genetic studies using relevant HBV infection and replication models further characterize the interplays between the cis-element within the viral sequence and the trans-elements from the host factors. PAPD5/7 targeting inhibitors, with oral bioavailability, thus represent an opportunity to reduce HBsAg through destabilizing HBV RNA.
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http://dx.doi.org/10.1128/JVI.00574-21DOI Listing
June 2021

Synthesis and Evaluation of Chiral Rhodanine Derivatives Bearing Quinoxalinyl Imidazole Moiety as ALK5 Inhibitors.

Med Chem 2021 Jun 28. Epub 2021 Jun 28.

Molecular Medicine Research Center, College of Phamacy, Yanbian University, Yanji 133002, China.

Background: TGF-β signaling pathway inhibition is considered an effective way to prevent the development of several diseases. In the design and synthesis of TGF-β inhibitors, a rhodanine compound containing a quinoxalinyl imidazole moiety was found to have strong antimicrobial activity.

Objective: The purpose of this work was to investigate the antimicrobial activity of other chiral rhodanine TGF-β inhibitors synthesized.

Methods: Two series of 3-substituted-5-((5-(6-methylpyridin-2-yl)-4-(quinoxalinyl-6-yl)- 1H-imidazol-2-yl)methylene)-2-thioxothiazolin-4-ones (12a-h and 13a-e) were synthesized and evaluated for their ALK5 inhibitory and antimicrobial activity. The structures were confirmed by their 1H NMR, 13C NMR, and HRMS spectra. All the synthesized compounds were screened against Gram-positive strains, Gram-negative strains, and fungi.

Results: Among the synthesized compounds, compound 12h showed the highest activity (IC50 = 0.416 μM) against ALK5 kinase. Compound 12h exhibited a good selectivity index of > 24 against p38α MAP kinase and was 6.0-fold more selective than the clinical candidate, compound 2 (LY-2157299). Nearly all the compounds displayed high selectivity toward both Gram-positive and Gram-negative bacteria. They also showed similar or 2.0-fold greater antifungal activity (minimum inhibitory concentration [MIC] = 0.5 µg/mL) compared with the positive control compounds Gatifloxacin (MIC = 0.5 µg/mL) and fluconazole (MIC = 1 µg/mL).

Conclusion: The findings suggest that the synthesized rhodanine compounds have good ALK5 inhibitory activity and can be used for further research and development as potential antifungal drugs.
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http://dx.doi.org/10.2174/1573406417666210628144849DOI Listing
June 2021

Tissue Transglutaminase Impairs HTR-8/SVneo Trophoblast Cell Invasion via the PI3K/AKT Signaling Pathway.

Gynecol Obstet Invest 2021 17;86(3):264-272. Epub 2021 Jun 17.

Department of Obstetrics, Guangzhou Medical University Affiliated Guangzhou Women and Children's Medical Center, Guangzhou, China.

Objectives: The pathogenesis of preeclampsia (PE) is associated with impaired trophoblast invasion, which results in placental insufficiency. Our earlier studies demonstrated that tissue transglutaminase (tTG) is highly expressed in human PE serum. However, whether tTG participates in trophoblast invasion remains unclear. The aim of the present study was to determine the role and mechanism of tTG in regulating matrix metalloproteinase (MMP)-2/MMP-9 expression to reduce trophoblast invasiveness in PE.

Methods: HTR-8/SVneo cells were transfected with a lentivirus vector and small interfering RNA targeting tTG. The protein level was detected by Western blotting. Cell proliferation and apoptosis were assessed by MTS and flow cytometry assays, respectively. Cell invasion was investigated by Transwell assay. In addition, the influence of tTG on PI3K and AKT mRNA levels in HTR-8/SVneo cells was evaluated using reverse transcription-quantitative PCR.

Results: tTG-overexpression inhibited HTR-8/SVneo cell proliferation and invasion and promoted apoptosis. In addition, upregulation of tTG induced an increase of PI3K and phosphorylated AKT and a decrease of MMP-2 and MMP-9 expression. tTG-knockdown significantly promoted the proliferation and invasion of HTR-8/SVneo cells and inhibited the apoptosis. Furthermore, the PI3K expression level was reduced, and the MMP-2/MMP-9 protein levels were increased.

Conclusion: Taken together, the present study demonstrated that tTG-overexpression inhibited HTR-8/SVneo cell invasion via reducing the expression of MMP-2 and MMP-9 by activating PI3K/AKT signaling pathway, which may lead to the occurrence or development of PE. The present data provide new insights into modulation of tTG expression as a potential therapeutic target for PE.
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http://dx.doi.org/10.1159/000515086DOI Listing
June 2021

Dietary potato intake and risks of type 2 diabetes and gestational diabetes mellitus.

Clin Nutr 2021 Jun 1;40(6):3754-3764. Epub 2021 May 1.

School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an, China. Electronic address:

Background & Aims: Evidence regarding associations between potato consumption and type 2 diabetes (T2D) and gestational diabetes mellitus (GDM) risks is accumulating. This study aims to synthesize the evidence by conducting a meta-analysis of available studies.

Methods: PubMed, Web of Science, EMBASE and Cochrane Library were searched (up to August 2020) to retrieve all eligible studies on the associations of interest. The risk estimates with 95% confidence intervals (CIs) were summarized using random- or fixed-effects model based on heterogeneity. Meta-analyses were performed for East and West regions separately. Dose-response relationship was assessed using data from all intake categories in each study.

Results: A total of 19 studies (13 for T2D; 6 for GDM) were identified, including 21,357 T2D cases among 323,475 participants and 1516 GDM cases among 29,288 pregnancies. Meta-analysis detected a significantly positive association with T2D risk for total potato (RR: 1.19 [1.06, 1.34]), baked/boiled/mashed potato (RR: 1.08 [1.00, 1.16]), and French fries/fried potato (RR: 1.33 [1.03, 1.70]) intakes among Western populations. Dose-response meta-analysis demonstrated a significantly increased T2D risk by 10% (95% CI: 1.07, 1.14; P for trend<0.001), 2% (95% CI: 1.00, 1.04; P for trend = 0.02) and 34% (95% CI: 1.24, 1.46; P for trend<0.001) for each 80 g/day (serving) increment in total potato, unfried potato, and fried potato intakes, respectively. As for GDM, summarized estimates also suggested a higher though non-significant GDM risk for total potato (RR: 1.19 [0.89, 1.58]), and French fries/fried potato (RR: 1.03 [0.97, 1.09]) intakes in Western countries. In the dose-response meta-analysis, a significantly increased GDM risk was revealed for each daily serving (80 g) intakes of total potato (RR: 1.22; 95% CI: 1.06, 1.42; P for trend = 0.007) and unfried potato (RR: 1.26; 95% CI: 1.07, 1.48; P for trend = 0.006).

Conclusions: This study suggests that higher potato intake is associated with higher T2D risk among Western populations. The positive relationship presents a significant dose-response manner. Wisely controlled potato consumption may confer potential glucometabolic benefits.
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http://dx.doi.org/10.1016/j.clnu.2021.04.039DOI Listing
June 2021

Promotion of Pre-natal Education Courses Is Associated With Reducing the Rates of Caesarean Section: A Case-Control Study.

Front Public Health 2021 28;9:666337. Epub 2021 May 28.

The Hospital of Obstetrics & Gynaecology of Fudan University, Shanghai, China.

The number of women having a caesarean section has significantly increased worldwide, in particular in China. Maternal requestion makes a moderate contribution to this increased rate in China. Reducing the caesarean section rate is now becoming a big challenge to midwives and obstetricians as well as health policymakers in China. Our recent survey found that pre-natal education course had some positive effects on the reduction of caesarean section on maternal request. However, pre-natal education course is relatively new in China. In this study, we investigated whether pre-natal education course influences delivery mode in the largest tertiary women's hospital in China. In this retrospective study, during the study period, 644 pregnant women attended a pre-natal education course and 4,134 pregnant women did not. Data on maternal age, parity, gravida, delivery mode, delivery weeks, birthweight, gestational age at attending pre-natal education course and maternal body mass index before pregnancy were collected and analysed. The numbers of women who attempted vaginal delivery were significantly higher in women who attended a pre-natal education course, compared to women who did not (87 vs. 60%). In addition, the rate of caesarean section on maternal request was 23% in women who attended a pre-natal education course. Attendance of a pre-natal education course influences the mode of delivery and reduces the unnecessary caesarean section in China. Our findings suggest that the promotion of pre-natal education courses is important to reduce the higher caesarean section rate in China, by midwives or obstetricians or health policy-makers as part of China's strategy.
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http://dx.doi.org/10.3389/fpubh.2021.666337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192822PMC
June 2021

Lateral Habenula Serves as a Potential Therapeutic Target for Neuropathic Pain.

Neurosci Bull 2021 Jun 4. Epub 2021 Jun 4.

Department of Pharmacology and Department of Anesthesiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

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http://dx.doi.org/10.1007/s12264-021-00728-2DOI Listing
June 2021

Dietary calcium, magnesium, and phosphorus intakes and risk of stroke in Chinese adults.

Sci Rep 2021 May 28;11(1):11270. Epub 2021 May 28.

School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, People's Republic of China.

Controversial results have been reported about the association of calcium, magnesium, and phosphorus and stroke risk, but none in China. To investigate the association between dietary calcium, magnesium, phosphorus, and stroke incidence in Chinese adults, we collected data from the China Health and Nutrition Survey (CHNS) from 2004 to 2011, including 6411 participants aged 45-79 years and free of stroke at baseline. Diet was assessed by interviews combining 3-d 24-h food recalls and household food inventory weighing at each survey round. The stroke incident was identified based on the validated self-report. Multivariate Cox regression models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). For 32,024 person-years of follow-up, 179 stroke cases were documented. After adjustment for major lifestyle and dietary risk factors, calcium intake was positively associated with reduced stroke risk, and the HR of stroke comparing extreme quartiles was 0.53 (95% CI 0.29-0.96, P = 0.03). In further stratified analyses, significant heterogeneity across sex strata was found (P = 0.03). Dietary calcium intake among men was more inversely related to stroke, with HRs being 0.33 (95% CI 0.15-0.76, P  = 0.02), compared to 1.24 (95% CI 0.46-3.35, P = 0.89) among women. However, no significant association between stroke and magnesium or phosphorus was revealed. Our findings suggest that higher dietary calcium intake was associated with a lower risk of stroke in Chinese adults, particularly in men.
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http://dx.doi.org/10.1038/s41598-021-90388-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163833PMC
May 2021

Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level.

Front Endocrinol (Lausanne) 2021 7;12:679582. Epub 2021 May 7.

Womens Hospital of Nanjing Medical University, Nanjing, China.

Gestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding of the human placenta remains limited. In this study, we generated a comprehensive transcriptomic profile of cellular signatures and transcriptomes in the human placenta in GDM using single-cell RNA sequencing (scRNA-seq), constructed a comprehensive cell atlas, and identified cell subtypes and subtype-specific marker genes. In addition, we investigated the placental cellular function and intercellular interactions in GDM. These findings help to elucidate the molecular mechanisms of GDM, and may facilitate the development of new approaches to GDM treatment and prevention.
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http://dx.doi.org/10.3389/fendo.2021.679582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139321PMC
May 2021

Diagnostic roles of proliferative markers in pathological Grade of T1 Urothelial Bladder Cancer.

J Cancer 2021 5;12(9):2498-2506. Epub 2021 Mar 5.

Department of Pathology, Guangxi Medical University Cancer Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China.

The stage T1 urothelial bladder cancer (T1 UBC) tumor grade classification is important for prognosis and clinical management. However, the reproducibility of this two-grade classification system is limited in regards to pathological diagnosis, and there is lack of ideal, objective and easily detected markers for pathological diagnosis. In our study, bladder urothelial lesions from a total of 124 patients diagnosed pathologically after transurethral resection of the bladder tumor (TURBT) were collected, including non-cancerous lesions from 33 patients and lesions from 91 T1 UBC patients. A series of previous studies have suggested some common and valuable factors in the diagnosis and prognosis of UBC, but there are still some controversial factors, such as the mitotic figure (MF) of tumor cell, cell proliferation index Ki-67, graded differentiation marker CK20, P53, P504S and carcinogenesis associated telomerase reverse transcriptase (TERT) promoter mutations. The purpose of this study was to evaluate the value of these factors in the pathological grading diagnosis of T1 UBC. The results showed that gender, lesion size, mitotic index (MI), CK20, P53, Ki-67, P504S and TERT promoter hot spot mutations (C228T and C250T) were correlated with T1 UBC diagnosis (P<0.05). The MI, Ki-67 and P504S were correlated with the pathological grade of T1 UBC (P<0.05). Logistic regression analysis showed that the MI and Ki-67 were independent risk factors for high-grade (HG) of T1 UBC (P<0.05). The combined detection of the MI, Ki-67 and P504S in a multivariate diagnostic model improved the diagnostic accuracy of assigning the T1 UBC pathological grade (AUC=0.904, 95%CI: 0.824~0.956, P<0.05). In conclusion, MI and Ki-67, as important markers of histopathology and cell proliferation, can be easily measured and have good reproducibility. These markers may be meaningful parameters for assigning the pathological grade of UBC.
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http://dx.doi.org/10.7150/jca.52336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040703PMC
March 2021

Design of a multifunction novel flexible fault current limiter for AC distribution network.

PLoS One 2021 8;16(4):e0245956. Epub 2021 Apr 8.

School of Electrical Engineering and Automation, Fuzhou University, Fuzhou, Fujian, China.

Based on the separation voltage type of cascaded H bridge-modular multilevel converters (CHB-MMC) and current predictive model control (CPMC) technology, a novel flexible fault-current limiter (NFFCL) is firstly proposed for restraining the negative impact of the distribution network's disturbance in this paper. When a disturbance occurs, the inner-loop CPMC of the multilevel converters establish the value function to offer the specific current, thus increasing the voltage deviation at both ends of the series capacitor or generating reverse harmonic compensation voltage. In that case, three single-phase MNFFCLs can be regarded as variable voltage sources to eliminate the negative effects of faults or harmonics. Owing to the multi-capacitance series structure, the maximum voltage drops of the single capacitance can be predetermined by the number of capacitors. And with the low voltage drop of single capacitance, the output current of the CHB-MMC can also be controlled within an acceptable range. Through the simulation results, the disturbance's negative impact on the non-fault area can be eliminated almost 100%.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245956PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031456PMC
April 2021

Retracted: Mukonal Inhibits Cell Proliferation, Alters Mitochondrial Membrane Potential and Induces Apoptosis and Autophagy in Human CNE1 Nasopharyngeal Carcinoma Cells.

Med Sci Monit 2021 Mar 25;27:e932337. Epub 2021 Mar 25.

Department of Otolaryngology, The Second Hospital of Jilin University, Changchun, Jilin, China (mainland).

Retracted, due to breach of publishing guidelines, following the identification of non-original content. Reference: Mukonal Inhibits Cell Proliferation, Alters Mitochondrial Membrane Potential and Induces Apoptosis and Autophagy in Human CNE1 Nasopharyngeal Carcinoma Cells Yingyuan Guo, Yanru Hao, Guofang Guan, Shuaishuai Ma, Zhiling Zhu, Fang Guo, Jie Bai Med Sci Monit 2019; 25:1976-1983 10.12659/MSM.913915.
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http://dx.doi.org/10.12659/MSM.932337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009267PMC
March 2021

Biomechanical evaluation of a customized 3D-printed polyetheretherketone condylar prosthesis.

Exp Ther Med 2021 Apr 11;21(4):348. Epub 2021 Feb 11.

Department of Oral and Maxillofacial Surgery, School of Stomatology, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. China.

The present study aimed to evaluate the biomechanical behavior of a custom 3D-printed polyetheretherketone (PEEK) condylar prosthesis using finite element analysis and mechanical testing. The Mimics software was used to create a 3D model of the mandible, which was then imported into Geomagic Studio software to perform osteotomy of the lesion area. A customized PEEK condyle prosthesis was then designed and the finite element model of the PEEK condyle prosthesis, mandible and fixation screw was established. The maximum stress of the prosthesis and screws, as well as stress and strain of the cortical and cancellous bones in the intercuspal position, incisal clench, left unilateral molar clench and right unilateral molar clench was analyzed. The biomechanical properties of the prosthesis were studied using two models with different lesion ranges. To simulate the actual clinical situation, a special fixture was designed. The compression performance was tested at 1 mm/min for the condyle prosthesis, prepared by fused deposition modeling (FDM). The results of a finite element analysis suggested that the maximum stress of the condyle was 10.733 MPa and the maximum stress of the screw was 9.7075 MPa; both were far less than the yield strength of the material. The maximum force that the two designed prostheses were able to withstand was 3,814.7±442.6 N (Model A) and 4,245.7±348.3 N (Model B). Overall, the customized PEEK condyle prostheses prepared by FDM exhibited a uniform stress distribution and good mechanical properties, providing a theoretical basis for PEEK as a reconstruction material for repairing the temporomandibular joint.
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http://dx.doi.org/10.3892/etm.2021.9779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903381PMC
April 2021

Anthraquinone derivative C10 inhibits proliferation and cell cycle progression in colon cancer cells via the Jak2/Stat3 signaling pathway.

Toxicol Appl Pharmacol 2021 05 13;418:115481. Epub 2021 Mar 13.

Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.

Since its discovery, anthraquinone has become very valuable as a lead compound in the development of anti-cancer drugs. Previously, we designed and synthesized a new type of amide anthraquinone derivative (1-nitro-2-acylanthraquinone glycine, C10) with good activity against colon cancer. However, its effect and the underlying mechanism are unclear. In this study, C10 significantly inhibited the proliferation of HCT116 and HT29 colon cancer cells by blocking the cell cycle at the G2/M phase. C10 also plays a role in cell cycle arrest by reducing the protein and gene expression levels of cyclin B1 and its downstream signaling molecule cyclin-dependent kinase (CDK1). In addition, molecular docking studies showed that C10 has high affinity for Jak2, the first target in the cell cycle-related Jak2/Stat3 signaling pathway. Furthermore, C10 downregulated the expression of Jak2/Stat3 signaling pathway-related signaling molecules proteins and genes, and up-regulated the expression of PIAS-3, the upstream signaling molecule of Stat3, thereby down-regulating Stat3 phosphorylation. C10 reversed the expression of Jak2/Stat3 signaling pathway-related molecules activated by IL-6. Overall, our results indicate for the first time that C10 induces cell cycle arrest and inhibits cell proliferation by inhibiting the Jak2/Stat3 signaling pathway. This study provides new insights into the potential role of Jak2/Stat3 in the regulating cell cycle-related signaling pathways that mediate the inhibitory effects of C10 on colon cancer cell proliferation.
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http://dx.doi.org/10.1016/j.taap.2021.115481DOI Listing
May 2021

A novel missense mutation in causes a rare form of X-linked postlingual sensorineural hearing loss in a Chinese family.

Transl Pediatr 2021 Feb;10(2):378-387

Department of Otolaryngology, The Second Hospital of Jilin University, Changchun, China.

Background: X-linked deafness-4 (DFNX4) caused by the functional loss of the gene is one form of nonsyndromic hearing loss with postlingual onset. This study aimed to investigate the cause of X-linked inherited sensorineural nonsyndromic hearing loss in a four-generation Chinese family and to explain the reason for extremely different hearing phenotypes between the proband and other family members.

Methods: Whole-exome sequencing (WES) and co-segregation analysis were used to identify the pathogenic variants. Furthermore, methylation differences among the androgen receptor genes were utilized to investigate whether the severe phenotype of the proband is related to X-chromosome inactivation (Xi).

Results: We described in detail the clinical characteristics of the family and identified a novel missense mutation (c.262C>G: p.Gln88Glu) in by WES. This variant was co-segregated with the postlingual hearing loss phenotype and was absent in 300 normal controls. Also, we found that the proband, a 4-year-old female, carries two new compound heterozygous mutations (c.9259G>A: p.Val3087Ile and c.8576G>A: p.Arg2859His) in the gene, but to date without any other symptoms except profound sensorineural hearing loss. Additionally, analysis of X-chromosome inactivation indicated moderate skewing in the proband, which is probably related to the heterogeneity of clinical characteristics.

Conclusions: This is the first study to report a missense mutation of in a Chinese family. Our findings have enriched the mutation and phenotypic spectrum of the gene.
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http://dx.doi.org/10.21037/tp-20-435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944167PMC
February 2021

A supramolecular complex of hydrazide-pillar[5]arene and bisdemethoxycurcumin with potential anti-cancer activity.

Bioorg Chem 2021 May 24;110:104764. Epub 2021 Feb 24.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, China. Electronic address:

Pillar[5]arene complexes of the naturally occurring compound bisdemethoxycurcumin (BDMC) were acquired for improving the water solubility and stability of BDMC. As a family member of curcuminoid compounds, BDMC has many interesting therapeutic properties. However, its low aqueous solubility and stability resulted in poor availability and restricted the clinical efficacy. Pillar[5]arenes with hydrophilic ends and a hydrophobic cavity could include with BDMC based on size matching. The synthesized hydrazide-pillar[5]arene (HP5A) and BDMC had a strong host-guest interaction with a 1:1 binding stoichiometry. Furthermore, the HP5A ⊃ BDMC complex could self-assemble into well-defined fibers in water/ethanol solution. This supramolecular complex worked well in vitro for inhibiting the proliferation of hepatoma carcinoma cells HepG2. Remarkably, this method of complexation with pillar[5]arenes visibly reduced the undesirable side effects on normal cells without weakening the anti-cancer activity of the drugs. We expected that the obtained host-guest complex and fibrous assembly would provide a promising platform for delivering drugs with low water solubility.
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http://dx.doi.org/10.1016/j.bioorg.2021.104764DOI Listing
May 2021

Correlation of placenta previa type with cesarean section blood loss and predictors of hysterectomy.

Chin Med J (Engl) 2021 Jan 27;134(4):501-502. Epub 2021 Jan 27.

Department of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai Institute of Ultrasound in Medicine, Shanghai 200233, China.

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http://dx.doi.org/10.1097/CM9.0000000000001210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909324PMC
January 2021

Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination.

Nat Commun 2021 02 19;12(1):1171. Epub 2021 Feb 19.

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Direct cloning represents the most efficient strategy to access the vast number of uncharacterized natural product biosynthetic gene clusters (BGCs) for the discovery of novel bioactive compounds. However, due to their large size, repetitive nature, or high GC-content, large-scale cloning of these BGCs remains an overwhelming challenge. Here, we report a scalable direct cloning method named Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination (CAPTURE) which consists of Cas12a digestion, a DNA assembly approach termed T4 polymerase exo + fill-in DNA assembly, and Cre-lox in vivo DNA circularization. We apply this method to clone 47 BGCs ranging from 10 to 113 kb from both Actinomycetes and Bacilli with ~100% efficiency. Heterologous expression of cloned BGCs leads to the discovery of 15 previously uncharacterized natural products including six cyclic head-to-tail heterodimers with a unique 5/6/6/6/5 pentacyclic carbon skeleton, designated as bipentaromycins A-F. Four of the bipentaromycins show strong antimicrobial activity to both Gram-positive and Gram-negative bacteria such as methicillin-resistant Staphylococcus aureus, vancomycinresistant Enterococcus faecium, and bioweapon Bacillus anthracis. Due to its robustness and efficiency, our direct cloning method coupled with heterologous expression provides an effective strategy for large-scale discovery of novel natural products.
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http://dx.doi.org/10.1038/s41467-021-21275-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896053PMC
February 2021

Co-benefits of peaking carbon dioxide emissions on air quality and health, a case of Guangzhou, China.

J Environ Manage 2021 Mar 18;282:111796. Epub 2021 Jan 18.

Environmental Defense Fund, Beijing, 100007, China.

Cities play a key role in making carbon emission reduction targets achievable and tackling air pollution. Using Guangzhou city as a case, this paper explored the air quality and health co-benefits of peaking carbon dioxide emissions under three scenarios and developed an integrated assessment framework by combining a local air pollutant emission inventory, an atmospheric chemistry transport model, and a health assessment model. The results showed that SO, PM, and PM could achieve larger emission reductions than NH, VOCs, and NOx among all the scenarios we examined. Under the enhanced peaking scenario with the most stringent mitigation strategies, Guangzhou could meet the local ambient air quality standard for PM (34 μg/m), with the most reduction observed in the annual average PM concentration (28.4%) and related premature deaths (17.08%), compared with the base year 2015. We also identified hotspot grids, which were areas with high concentrations of carbon emissions, high concentrations of air pollution and poor air quality in Guangzhou. Our analysis highlighted the importance of promoting peaking carbon dioxide emission for the improvement of air quality and public health at the city level.
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http://dx.doi.org/10.1016/j.jenvman.2020.111796DOI Listing
March 2021

Trends of temperature variability: Which variability and what health implications?

Sci Total Environ 2021 May 6;768:144487. Epub 2021 Jan 6.

School of Physics, Peking University, Beijing, China.

A large majority of climate change studies carried out to date are on changes in mean climate, which have comparatively downplayed variability. In terms of trend analysis or forecast, the scientific output and common knowledge for global warming are much more robust than for changes in temperature variability. Quantification of temperature variability adds another dimension of temporal scale, requiring immense labor and presenting great uncertainty. Regardless, this endeavor is necessary since changes in ambient temperature variabilities could also contribute to current and future human health burden besides changes in mean quantities. Here, we review the current literature on trends of surface air temperature variability defined at a range of timescales, aiming to tease out the welter of evidence and thus improving the scientific recognition of changes in air temperature variability in the context of climate change. The findings of reviewed studies from numerous regions differ substantially over various temporal scales. In general, the ambient temperature variability on short time scales (e.g., diurnal or inter-day) shows a downward trend, while it is increasing on longer time scales (e.g., inter-annual). We then move beyond the review and deliver an extended discussion of potential implications for future research related to ambient temperature variability. We highlight the need to consider the methodological choices, especially timescales of interest, in the trend analysis as well as health impact studies. Continued research focusing on temperature variability at multiple timescales, with concerted efforts from scientists of all relevant stripes, is meaningful in synthesizing knowledge and reducing uncertainties surrounding air temperature variability.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144487DOI Listing
May 2021

Fever-range hyperthermia promotes cGAS-STING pathway and synergizes DMXAA-induced antiviral immunity.

Int J Hyperthermia 2021 ;38(1):30-37

Key Laboratory of System Biomedicine of the Ministry of Education, Shanghai Jiao Tong University, Shanghai, PR China.

Fever-range hyperthermia or fever-range temperature (hereafter FRT) improves survival and shortens disease duration in microbial infections. However, the mechanisms of these beneficial effects still remain elusive. We hypothesized that FRT might enhance cell responsiveness to infections by promoting cGAS-STING signaling to cause enhanced production of IFN-β. To investigate the effect fever-range hyperthermia on cGAS-STING pathway. RAW 264.7 and cGAS RAW 264.7 cells, stimulated with 5μg/ml herring testis DNA (htDNA), were heated to 39.5°C and analyzed for the expression of cGAS, STING, IFN-β, and the synthesis of cGAMP and IRF3 phosphorylation. In vivo, wild type C57BL/6J mice were subjected to whole body hyperthermia (WBH) at 39.5°C. The mice were then challenged with influenza virus and analyzed for antiviral response in term of IFN-β expression, body weight and survival. We found that 39.5°C FRT upregulated the expression of cGAS and STING, and induced the synthesis of cGAMP and production of IFN-β in htDNA-transfected RAW 264.7 cells more potently as compared to 37°C. Moreover, FRT+DMXAA-treated cells were better protected from vesicular stomatitis virus (VSV)-induced cytotoxicity in vitro in contrast to the nonprotected control (no FRT and DMXAA) or DMXAA treatment alone. In vivo, FRT at 39.5°C, co-administered with DMXAA, significantly induced the expression of IFN-β, showed reduced weight loss mice and exhibited 25% more survival over the course of 14 days as compared to DMXAA treated mice 37°C. We conclude that fever-range hyperthermia promotes cGAS-STING pathway to cause increased expression of IFN-β and mediate its antiviral effects.
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http://dx.doi.org/10.1080/02656736.2020.1868582DOI Listing
July 2021

Associations between first-trimester intrauterine hematoma and twin pregnancy outcomes: a retrospective cohort study.

BMC Pregnancy Childbirth 2021 Jan 11;21(1):46. Epub 2021 Jan 11.

Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, Guangdong Province, China.

Background: In recent years, first-trimester intrauterine hematoma (IUH) has become increasingly common in twin pregnancy. The majority of studies on IUH have excluded twin pregnancies, and others did not differentiate between singleton and twin pregnancies. The impact of IUH on twin pregnancy is unclear. Therefore, the primary objective of our study was to examine associations between first-trimester IUH and pregnancy outcomes in twin pregnancies.

Methods: The data of 1020 twin pregnancies in women who received a routine examination from January 2014 to December 2018 were reviewed. We compared baseline data and pregnancy outcomes between those with and without IUH. Multivariable logistic regression analysis was used to adjust for possible confounding factors.

Results: A total of 209 patients (21.3%) developed IUH in the first trimester. First-trimester IUH was significantly associated with increased odds of miscarriage (adjusted odds ratio 14.27, 95% CI 8.25-24.70) and vanishing twin syndrome (adjusted odds ratio 3.26, 95% CI 1.11-4.61). However, there were no differences in the rates of stillbirth, preeclampsia, preterm labor (< 34 weeks), low birth weight, postpartum hemorrhage or fetal distress between the two groups. Maternal age, previous preterm birth, chorionicity in twins and the gestational week at first ultrasound did not differ between the two groups. The women with IUH had high rates of previous miscarriage (46.73% vs 38.37%, p = 0.01), assisted conception (48.56% vs 32.60%, p < 0.001) and accompanied vaginal bleeding (67.46% vs 13.43%, p < 0.001). According to the logistic regression analyses, these characteristics were not associated with pregnancy loss or vanishing twin syndrome. No IUH characteristics, including volume, largest diameter, or the presence of vaginal bleeding, were associated with pregnancy loss or vanishing twin syndrome before 20 weeks of gestation (P > 0.05).

Conclusion: In women with twin pregnancy, the presence of IUH in the first trimester was associated with the loss of one or both fetuses before 20 weeks of gestation. However, previous miscarriage, the conception method, the IUH size and the presence of vaginal bleeding were not independently associated with miscarriage or vanishing twin syndrome.
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http://dx.doi.org/10.1186/s12884-020-03528-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802326PMC
January 2021

Laboratory Management and Quality Control Practice of SARS-CoV-2 Nucleic Acid Detection.

Lab Med 2021 Jan;52(1):e8-e14

Department of Pathology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: A positive result of SARS-CoV-2 nucleic acid detection provides critical laboratory evidence for clinical confirmed diagnosis, pandemic status evaluation, a pandemic prevention plan, treatment of infected people with symptoms, and protection of uninfected people. This study aims to provide a practical reference for SARS-CoV-2 nucleic acid-related research and detection.

Methods: Our laboratory has established policies combining personnel management and quality control practices for SARS-CoV-2 nucleic acid detection during the pandemic.

Results: In this article, we describe cross-department personnel management and key points of personal protection and quality control in the testing process. We also report on the differences in detection and the compatibility between different brand kits.

Conclusion: It is critical to maintain a standard and accurate laboratory operation for nucleic acid testing.
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http://dx.doi.org/10.1093/labmed/lmaa077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665693PMC
January 2021

Long-term exposure to fine particulate matter and dementia incidence: A cohort study in Hong Kong.

Environ Pollut 2021 Feb 25;271:116303. Epub 2020 Dec 25.

School of Public Health, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China.

Recent studies suggested that long-term exposure to fine particulate matter (PM) was related to a higher risk of dementia incidence or hospitalizations in western populations, but the evidence is limited in Asian cities. Here we explored the link between long-term PM exposure and dementia incidence in the Hong Kong population and whether it varied by population sub-group. We utilized a Hong Kong Chinese cohort of 66,820 people aged ≥65 years who were voluntarily enrolled during 1998-2001 and were followed up to 2011. Prevalent dementia cases were excluded based on the face-to-face interview at baseline. We ascertained the first occurrence of hospitalization for all-cause dementia and major subtypes during the follow-up period. We assessed PM concentrations using a satellite data-based model with a 1 × 1 km resolution on the residential address. Cox proportional hazards models were adopted to estimate associations of annual mean PM exposure with dementia incidence, adjusting for potential confounders. We identified 1183 incident cases of all-cause dementia during the follow-up period, of which 655 (55.4%) were cases of Alzheimer's disease, and 334 (28.2%) were those of vascular dementia. We found a positive association between annual mean PM exposure and all-cause dementia incidence in the fully adjusted model. The estimated hazard ratio was 1.06 (95% confidence interval (CI): 1.00, 1.13) per every 3.8 μg/m increase in annual mean PM exposure. And the estimated HRs for Alzheimer's disease and vascular dementia were 1.03 (95% CI: 0.94, 1.12) and 1.09 (95% CI: 0.98, 1.22), respectively. We did not find effect modifications by age, sex, BMI, hypertension, diabetes, or heart disease on the associations. Results suggest that long-term exposure to PM is associated with a higher risk of dementia incidence in the Asian population.
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http://dx.doi.org/10.1016/j.envpol.2020.116303DOI Listing
February 2021

[Application and analysis of medicinal plant resources of six major ethnic minorities living in Xishuangbanna].

Zhongguo Zhong Yao Za Zhi 2020 Oct;45(20):5034-5041

Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Jinghong 666100, China.

The study aims at exploring the composition and characteristics of the medicinal plant resources of six major ethnic minorities living in Xishuangbanna, as well as the characteristics of traditional utilization of ethnic medicine, and promote the rational protection and inheritance and utilization of ethnic medicine resources and traditional knowledge. Based on the fourth survey of traditional Chinese medicine resources in Xishuangbanna, Yunnan province, through the methods of literature collection, interviewing and field investigation, this paper analyzed the data of the types, medicinal parts and attending diseases of medicinal plant resources of Dai, Hani, Jinuo, Wa, Lahu and Bulang nationalities. The results showed that there were 1 048 medicinal plant resources of the six ethnic minorities, and the medicinal plants were 735, 497, 272, 295, 280 and 49 in the six nationalities, respectively. The cross-use of medicinal plants among the six nationalities accounted for 62.45%, 78.07%, 88.60%, 87.46%, 82.25% and 95.92%, respectively. The main medicinal plants used by the six major ethnic minorities are root and rhizome, whole grass, leaf and stem. Various ethnic medicinal plants are mainly used in the treatment of diseases of digestive system, respiratory system, rheumatic disease, trauma, snake and insect bite and other external diseases. This study showed that Xishuangbanna has rich resources of ethnic medicinal plants. The ethnic medicine culture with Dai medicine as the main part has a long history and diversified theories, forming a unique ethnic characteristic culture. However, all ethnic medicine resources and the application of traditional knowledge are faced with the risk of gradually decreasing, it is suggested to strengthen the mining, Xishuangbanna folk medicine of ethnic culture protection and inheri-tance, and to develop regional economy and the international community has a positive influence.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200628.101DOI Listing
October 2020

Study on the Synthesized Rosin Glyceride over LaZSM-5 Zeolite Catalyst Synthesized by the in Situ Method.

ACS Omega 2020 Dec 7;5(49):31543-31550. Epub 2020 Dec 7.

Taiyuan University of Science and Technology, College of Environment and Safety, Taiyuan 030024, PR China.

LaZSM-5 zeolite was synthesized by the in situ method and used as catalysts to catalyze the synthesis of rosin glyceride. As a comparison, ZSM-5 was also synthesized and used as catalysts to catalyze the synthesis of rosin glyceride. The synthesized ZSM-5 and LaZSM-5 zeolite catalysts were characterized and analyzed. The experimental results showed that the in situ synthesis of LaZSM-5 made La into the skeleton of ZSM-5 zeolite and increased the amount of Lewis acid on the LaZSM-5 zeolite. Also, Lewis acid was the key to liquid-phase esterification reaction. Compared with ZSM-5 zeolite, LaZSM-5 zeolite contributed to a higher yield and better stability as a catalyst for the synthesis of rosin glycerides.
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http://dx.doi.org/10.1021/acsomega.0c03427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745225PMC
December 2020
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