Publications by authors named "Fang Deng"

174 Publications

Analysis of the characteristic compounds of Citri Sarcodactylis Fructus from different geographical origins.

Phytochem Anal 2021 Jun 10. Epub 2021 Jun 10.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Introduction: Citri Sarcodactylis Fructus (CSF) is widely used as a food ingredient and a traditional Chinese medicine. In China, CSF is cultivated in many places, including Sichuan, Guangdong, Zhejiang, and Fujian provinces. The types and chemical contents of CSF from different origins may vary greatly due to the difference in climate and environmental conditions. Therefore, comparing the chemical composition of CSF from various places is vital.

Objective: To rapidly select potential characteristic compounds for differentiating CSF from different origins.

Material And Methods: Thirty-one batches of CSF samples from different regions were analysed using ultra-performance liquid chromatography with hybrid quadrupole-orbitrap high-resolution mass spectrometry. Thereafter, chemometric methods, including principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA), were employed to find differential metabolites among the CSF samples from various origins.

Results: PCA revealed 77.9% of the total variance and divided all CSF samples into three categories corresponding to their origins. OPLS-DA displayed better discrimination of CSF from different sources, with R X, R Y, and Q of 0.801, 0.985, and 0.849, respectively. Finally, 203 differential metabolites were obtained from CSF from different origins using the variable importance in projection of the OPLS-DA model, 30 of which were identified, and five coumarin compounds were selected as marker compounds discriminating CSF from different origins.

Conclusion: This work provides a practical strategy for classifying CSF from different origins and offers a research foundation for the quality control of CSF.
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http://dx.doi.org/10.1002/pca.3069DOI Listing
June 2021

RNF34 ablation promotes cerebrovascular remodeling and hypertension by increasing NADPH-derived ROS generation.

Neurobiol Dis 2021 May 18;156:105396. Epub 2021 May 18.

Department of Neurology, The First Teaching Hospital of Jilin University, Changchun 130021, Jilin, China. Electronic address:

Cerebrovascular remodeling is the most common cause of hypertension and stroke. Ubiquitin E3 ligase RING finger protein 34 (RNF34) is suggested to be associated with the development of multiple neurological diseases. However, the importance of RNF34 in cerebrovascular remodeling and hypertension is poorly understood. Herein, we used mice with a global RNF34 knockout as well as RNF34 floxed mice to delete RNF34 in endothelial cells and smooth muscle cells (SMCs). Our results showed that global RNF34 knockout mice substantially promoted angiotensin II (AngII)-induced middle cerebral artery (MCA) remodeling, hypertension, and neurological dysfunction. Endothelial cell RNF34 did not regulate the development of hypertension. Rather, SMC RNF34 expression is a critical regulator of hypertension and MCA remodeling. Loss of RNF34 enhanced AngII-induced mouse brain vascular SMCs (MBVSMCs) proliferation, migration and invasion. Furthermore, MCA and MBVSMCs from SMC RNF34-deficient mice showed increased superoxide anion and reactive oxygen species (ROS) generation as well as nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, but exhibited no marked effect on mitochondria-derived ROS. Knockout of RNF34 promoted p22 expression, leading to increased binding of p22/p47 and p22/NOX2, and eventually NADPH oxidase complex formation. Immunoprecipitation assay identified that RNF34 interacted with p22. RNF34 deletion increased p22 protein stability by inhibiting ubiquitin-mediated degradation. Blockade of NADPH oxidase activity or knockdown of p22phox significantly abolished the effects of RNF34 deletion on cerebrovascular remodeling and hypertension. Collectively, our study demonstrates that SMC RNF34 deficiency promotes cerebrovascular SMC hyperplasia and remodeling by increased NADPH-derived ROS generation via reducing p22 ubiquitin-dependent degradation.
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http://dx.doi.org/10.1016/j.nbd.2021.105396DOI Listing
May 2021

Overexpression of IL-8 promotes cell migration via PI3K-Akt signaling pathway and EMT in triple-negative breast cancer.

Pathol Res Pract 2021 Jul 20;223:152824. Epub 2020 Jun 20.

Department of Laboratory Medicine, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong, Chongqing 400016, PR China. Electronic address:

Triple-negative breast cancer (TNBC) is a type of malignant and heterogeneous tumor in premenopausal females with ineffective therapeutic targets. IL-8 is one of the earliest discovered chemotaxis cytokines which expression is closely related to the progress of various cancers. Previous studies show that IL-8 determines the prognosis of TNBC patients, nevertheless how IL-8 influence the progress of TNBC is unclear. In our studies, we discovered that overexpression of IL-8 promotes TNBC cells (TNBCs) migration and tumor growth via the PI3K-Akt and MAPK signaling pathway. Cell-cycle of TNBCs arrest at S phase by overexpression of IL-8, however, there is no significant difference on the cell viability and cell apoptosis of TNBCs. Besides, overexpression of IL-8 result in the downregulation of E-cadherin and the upregulation of Cyclin B1 in MDA-MB-231 cells. Taken together, our results suggest that IL-8 plays a crucial role in the progress of TNBC, and it could be a novel therapeutic target of TNBC.
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http://dx.doi.org/10.1016/j.prp.2020.152824DOI Listing
July 2021

A multi-tissue multi-omics analysis reveals distinct kineztics in entrainment of diurnal transcriptomes by inverted feeding.

iScience 2021 Apr 19;24(4):102335. Epub 2021 Mar 19.

Department of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Time of eating synchronizes circadian rhythms of metabolism and physiology. Inverted feeding can uncouple peripheral circadian clocks from the central clock located in the suprachiasmatic nucleus. However, system-wide changes of circadian metabolism and physiology entrained to inverted feeding in peripheral tissues remain largely unexplored. Here, we performed a 24-h global profiling of transcripts and metabolites in mouse peripheral tissues to study the transition kinetics during inverted feeding, and revealed distinct kinetics in phase entrainment of diurnal transcriptomes by inverted feeding, which graded from fat tissue (near-completely entrained), liver, kidney, to heart. Phase kinetics of tissue clocks tracked with those of transcriptomes and were gated by light-related cues. Integrated analysis of transcripts and metabolites demonstrated that fatty acid oxidation entrained completely to inverted feeding in heart despite the slow kinetics/resistance of the heart clock to entrainment by feeding. This multi-omics resource defines circadian signatures of inverted feeding in peripheral tissues (www.CircaMetDB.org.cn).
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http://dx.doi.org/10.1016/j.isci.2021.102335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050734PMC
April 2021

Improving feature selection performance for classification of gene expression data using Harris Hawks optimizer with variable neighborhood learning.

Brief Bioinform 2021 Apr 20. Epub 2021 Apr 20.

Department of Pathology and Pathophysiology, Hunan Normal University School of Medicine, Hunan Normal University, China.

Gene expression profiling has played a significant role in the identification and classification of tumor molecules. In gene expression data, only a few feature genes are closely related to tumors. It is a challenging task to select highly discriminative feature genes, and existing methods fail to deal with this problem efficiently. This article proposes a novel metaheuristic approach for gene feature extraction, called variable neighborhood learning Harris Hawks optimizer (VNLHHO). First, the F-score is used for a primary selection of the genes in gene expression data to narrow down the selection range of the feature genes. Subsequently, a variable neighborhood learning strategy is constructed to balance the global exploration and local exploitation of the Harris Hawks optimization. Finally, mutation operations are employed to increase the diversity of the population, so as to prevent the algorithm from falling into a local optimum. In addition, a novel activation function is used to convert the continuous solution of the VNLHHO into binary values, and a naive Bayesian classifier is utilized as a fitness function to select feature genes that can help classify biological tissues of binary and multi-class cancers. An experiment is conducted on gene expression profile data of eight types of tumors. The results show that the classification accuracy of the VNLHHO is greater than 96.128% for tumors in the colon, nervous system and lungs and 100% for the rest. We compare seven other algorithms and demonstrate the superiority of the VNLHHO in terms of the classification accuracy, fitness value and AUC value in feature selection for gene expression data.
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http://dx.doi.org/10.1093/bib/bbab097DOI Listing
April 2021

Compromised Dynamic Cerebral Autoregulation in Patients With Central Disorders of Hypersomnolence.

Front Neurol 2021 11;12:634660. Epub 2021 Mar 11.

Department of Neurology, Clinical Trial and Research Center for Stroke, The First Hospital of Jilin University, Changchun, China.

We aimed to investigate the dynamic cerebral autoregulation (dCA) in patients with central disorders of hypersomnolence during wakefulness. Thirty-six patients with central disorders of hypersomnolence were divided into three groups according to polysomnography and multiple sleep latency test results: the idiopathic hypersomnia group (IH), narcolepsy type 1 without rapid-eye-movement sleep behavior disorder group (NT1-RBD), and narcolepsy type 1 with rapid-eye-movement sleep behavior disorder group (NT1 + RBD), with 12 patients in each group. Twelve sex- and age-matched healthy controls were recruited. We assessed the Epworth sleepiness scale (ESS) and dCA of all subjects. dCA was assessed by analyzing the phase difference (PD) using transfer function analysis. The ESS and dCA were analyzed before and after standardized treatment in 24 patients with narcolepsy type 1. The overall PD of the IH, NT1-RBD, and NT1 + RBD groups were lower than that of the control group ( < 0.001). There were no significant differences between the overall PD of the NT1-RBD and NT1 + RBD group ( > 0.05). The ESS scores decreased and the overall PD increased after treatment in 24 patients with narcolepsy type 1 ( < 0.001). Multivariable analysis showed that mean sleep latency in multiple sleep latency test was independently associated with impaired overall PD ( < 0.05). The dCA is impaired in patients with central disorders of hypersomnolence. The impairment of dCA occurs irrespective of NT1-RBD/+RBD. The ESS score and dCA improved in patients with narcolepsy type 1 after medication treatment. The mean sleep latency in multiple sleep latency test was independently associated with impaired dCA. www.ClinicalTrials.gov, identifier: NCT02752139.
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http://dx.doi.org/10.3389/fneur.2021.634660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991911PMC
March 2021

Single Dose of Rituximab in Children with Steroid-Dependent / Frequently Relapsing Nephrotic Syndrome, Clinical Efficacy and Evaluation of Health-Related Quality of Life.

Iran J Kidney Dis 2021 Mar;1(2):109-115

Department of Nephrology, Children's Hospital of Anhui Medical University, Anhui, China.

Introduction: Steroid-dependent (SD)/frequently relapsing (FR) nephrotic syndrome (NS) follows a relapsing and remitting course. It is also characterized by proteinuria and edema, which can significantly affect health-related quality of life (HRQOL) in children. This study evaluated the effectiveness and safety of a single dose of rituximab (RTX) as well as the impact of RTX on HRQOL in children with SDFRNS.

Methods: Sixteen children with SDFRNS were enrolled in the study. Each patient was administered a single intravenous dose of RTX (375 mg/m2). Effectiveness was defined as remission of proteinuria. The side effects of RTX were monitored. HRQOL was assessed using PedsQL™ 4.0 Generic Core Scales.

Results: All the patients completed the study. Three SDNS patients and three FRNS patients discontinued treatment over 1 to 3.25 years of follow-up. Additionally, three SDNS patients and three FRNS patients experienced 1 to 2 relapses. The mean relapse-free period was 79.0 ± 77.6 days. The mean dosages of prednisolone and other immunosuppressants required were significantly lower (P < .05, < .001) six months after treatment with RTX compared with six months before treatment. Relapse rate was significantly reduced (P < .001) after treatment with RTX. Skin rash, hypotension, and fever were observed in one child. Total health score and physical, emotional, and school functioning were significantly higher six months after treatment with RTX (P < .001).

Conclusion: A single dose of RTX is effective and safe for children with SDFRNS and can improve HRQOL, especially physical, emotional, and school functioning.
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March 2021

TSH adenoma and syndrome of resistance to thyroid hormones-Two cases report of syndrome of inappropriate secretion of thyrotropin.

Brain Behav 2021 May 10;11(5):e02081. Epub 2021 Mar 10.

Breast and Thyroid Surgical Department, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, China.

SITSH (syndrome of inappropriate secretion of thyrotropin) is a rare clinical state defined as uninhibited serum thyroid stimulating hormone in the presence of elevated thyroid hormone. This state is complicated and mainly caused by the abnormal feedback of hypothalamus-pituitary thyroid axis. The TSH adenoma (TSH-oma) and resistance to thyroid hormones (RTH) are the main etiologies of SITSH. As is well known that the treatment strategies of RTH and TSH-oma are apparently different, thus identifying the difference between RTH and TSH-oma is of great significance for the diagnosis and treatment of SITSH. CASE DESCRIPTION: A 62-year-old man with a state of elevated thyroid hormones and inappropriate elevated serum TSH level was hospitalized in 2016. Results of the pituitary enhanced magnetic resonance imaging and the somatostatin test respectively demonstrated a space-occupying lesion of pituitary and an elevated serum sex hormone binding globulin (SHBG) and inhibited TSH secretion, which indicated the occurrence of TSH-oma. In 2019, a 23-year-old girl with a state of elevated thyroid hormones and inappropriate normal serum TSH was hospitalized. Interestingly, whole exome sequencing detection suggested a pathogenic mutation in thyroid hormone receptor β (THRB) gene, which has been shown to be associated with RTH. CONCLUSIONS: The difference between TSH-oma and RTH ought to be clarified for their accurate diagnose and treatment. The clinical experiences of the two cases reported here suggest that more detail information such as family medical history, serum SHBG level, and THRB gene test is helpful for the diagnose and treatment of TSH-oma and RTH. Additionally, we also summarized the identification points, diagnosis process, and treatment strategies for these two rare diseases.
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http://dx.doi.org/10.1002/brb3.2081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119795PMC
May 2021

Assembly and comparative analysis of the complete mitochondrial genome of Suaeda glauca.

BMC Genomics 2021 Mar 9;22(1):167. Epub 2021 Mar 9.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: Suaeda glauca (S. glauca) is a halophyte widely distributed in saline and sandy beaches, with strong saline-alkali tolerance. It is also admired as a landscape plant with high development prospects and scientific research value. The S. glauca chloroplast (cp) genome has recently been reported; however, the mitochondria (mt) genome is still unexplored.

Results: The mt genome of S. glauca were assembled based on the reads from Pacbio and Illumina sequencing platforms. The circular mt genome of S. glauca has a length of 474,330 bp. The base composition of the S. glauca mt genome showed A (28.00%), T (27.93%), C (21.62%), and G (22.45%). S. glauca mt genome contains 61 genes, including 27 protein-coding genes, 29 tRNA genes, and 5 rRNA genes. The sequence repeats, RNA editing, and gene migration from cp to mt were observed in S. glauca mt genome. Phylogenetic analysis based on the mt genomes of S. glauca and other 28 taxa reflects an exact evolutionary and taxonomic status of S. glauca. Furthermore, the investigation on mt genome characteristics, including genome size, GC contents, genome organization, and gene repeats of S. gulaca genome, was investigated compared to other land plants, indicating the variation of the mt genome in plants. However, the subsequently Ka/Ks analysis revealed that most of the protein-coding genes in mt genome had undergone negative selections, reflecting the importance of those genes in the mt genomes.

Conclusions: In this study, we reported the mt genome assembly and annotation of a halophytic model plant S. glauca. The subsequent analysis provided us a comprehensive understanding of the S. glauca mt genome, which might facilitate the research on the salt-tolerant plant species.
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http://dx.doi.org/10.1186/s12864-021-07490-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941912PMC
March 2021

Angiotensin (1-7) Attenuates Sepsis-Induced Acute Kidney Injury by Regulating the NF-κB Pathway.

Front Pharmacol 2021 4;12:601909. Epub 2021 Mar 4.

Department of Pathology, Anhui Provincial Children's Hospital, Hefei, China.

This study explores the protective mechanism of angiotensin (1-7) [Ang-(1-7)] on kidneys by examining its effects on renal histomorphology, inflammatory response, oxidative stress, and NF-κB signaling in mice suffering from sepsis-induced acute kidney injury. A sepsis-induced acute kidney injury mouse model was established by intracervically injecting lipopolysaccharides (LPS group), followed by the administration of Ang-(1-7) [LPS + Ang-(1-7) group]. The serum levels of urea nitrogen, creatinine and cystatin. c were measured with an automatic biochemical analyzer, and changes in proinflammatory cytokines and angiotensin II (Ang II) in the serum and kidneys were quantified by enzyme-linked immunosorbent assays. Changes in oxidative stress indices in the renal cortex were detected by colorimetry. The localization of Ang II in kidneys was examined by immunohistochemistry. Western blotting was used to examine phosphorylated NF-κB-p65 and IκBα levels in kidneys. Compared with the control group, the serum levels of urea nitrogen, creatinine and cystatin. c were increased, whereas the levels of Ang II, TNFα, IL-1β, IL-6, and malondialdehyde (mda) were increased significantly. The levels of Ang II and phosphorylated NF-κB-p65 were elevated in kidneys, whereas the levels of superoxide dismutase (sod), Total antioxidative capacity (TAOC), and inhibitor of NF-κB (IκBα) were reduced in the LPS group ( < 0.05). Pathological damage was also observed in kidneys of LPS-group mice. In Pearson correlation analysis, there was a positive correlation between Ang II and phosphorylated NF-κB-p65 levels, and a negative correlation between Ang II and IκBα levels ( < 0.05). After the application of Ang-(1-7), the levels of urea nitrogen, creatinine, cystatin. c, Ang II, TNFα, IL-1β, IL-6, and mda, as well as the expression of Ang II and phosphorylated NF-κB-p65 in kidneys of LPS + Ang-(1-7)-group mice, were lower than those in kidneys of LPS-group mice, but the levels of sod, TAOC, and IκBα were higher than those of LPS-group mice ( < 0.05). Pathological changes were less severe in mice of the LPS + Ang-(1-7) group. Overall, Ang-(1-7) can decrease the Ang II level, inhibit NF-κB signaling, reduce the inflammatory response, decrease oxidative stress, and mitigate sepsis-associated acute kidney injury.
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http://dx.doi.org/10.3389/fphar.2021.601909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970314PMC
March 2021

Thrombotic microangiopathy led to acute kidney injury in an infant with hemophagocytic lymphohistiocytosis: A case report.

Exp Ther Med 2021 Apr 24;21(4):396. Epub 2021 Feb 24.

Department of Nephrology, Anhui Provincial Children's Hospital, Hefei, Anhui 230022, P.R. China.

Hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal clinical syndrome frequently complicated by acute kidney injury (AKI) and acute tubular necrosis. Renal thrombotic microangiopathy (TMA) is a specific pathological feature of childhood HLH and few cases have been reported among infants. The present study presents a rare case of HLH with TMA in an infant. A 15-month-old infant with a week-long history of fever was admitted to hospital. The infant presented with AKI and subsequently a reduction in platelet and hemoglobin levels. TMA was diagnosed by kidney biopsy and the clinical, laboratory and bone marrow biopsy findings met the criteria of HLH. Due to a progressive increase in serum creatinine levels, hemodialysis was initiated on the second day following admission. Dexamethasone was administered to treat both the fever and HLH. The patient's body temperature returned to a normal range and platelet and hemoglobin levels were stable after 14 days of admission. Renal function stabilized on day 21. The results of genetic testing did not identify any disease-related variations. Childhood HLH is a severe condition and mortality can be reduced by early diagnosis and correct treatment. For patients with HLH and AKI, the possible role of TMA should be considered. Renal biopsy can help to identify the cause of AKI and can be performed when the patient's condition is stable.
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http://dx.doi.org/10.3892/etm.2021.9827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918195PMC
April 2021

A Novel Prognostic Model of Endometrial Carcinoma Based on Clinical Variables and Oncogenomic Gene Signature.

Front Mol Biosci 2020 7;7:587822. Epub 2021 Jan 7.

School of Mathematics and Statistics, Hunan Normal University, Changsha, China.

Due to the difficulty in predicting the prognosis of endometrial carcinoma (EC) patients by clinical variables alone, this study aims to build a new EC prognosis model integrating clinical and molecular information, so as to improve the accuracy of predicting the prognosis of EC. The clinical and gene expression data of 496 EC patients in the TCGA database were used to establish and validate this model. General Cox regression was applied to analyze clinical variables and RNAs. Elastic net-penalized Cox proportional hazard regression was employed to select the best EC prognosis-related RNAs, and ridge regression was used to construct the EC prognostic model. The predictive ability of the prognostic model was evaluated by the Kaplan-Meier curve and the area under the receiver operating characteristic curve (AUC-ROC). A clinical-RNA prognostic model integrating two clinical variables and 28 RNAs was established. The 5-year AUC of the clinical-RNA prognostic model was 0.932, which is higher than that of the clinical-alone (0.897) or RNA-alone prognostic model (0.836). This clinical-RNA prognostic model can better classify the prognosis risk of EC patients. In the training group (396 patients), the overall survival of EC patients was lower in the high-risk group than in the low-risk group [HR = 32.263, (95% CI, 7.707-135.058), = 8e-14]. The same comparison result was also observed for the validation group. A novel EC prognosis model integrating clinical variables and RNAs was established, which can better predict the prognosis and help to improve the clinical management of EC patients.
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http://dx.doi.org/10.3389/fmolb.2020.587822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817972PMC
January 2021

Attention-based parallel networks (APNet) for PM spatiotemporal prediction.

Sci Total Environ 2021 May 12;769:145082. Epub 2021 Jan 12.

School of Automation, Beijing Institute of Technology, Beijing 100081, China.

Urban particulate matter forecast is an important part of air pollution early warning and control management, especially the forecast of fine particulate matter (PM). However, the existing PM concentration prediction methods cannot effectively capture the complex nonlinearity of PM concentration, and most of them cannot accurately simulate the temporal and spatial dependence of PM concentration at the same time. In this paper, we propose an attention-based parallel network (APNet), which can extract short-term and long-term temporal features simultaneously based on the attention-based CNN-LSTM multilayer structure to predict PM concentration in the next 72 h. Firstly, the Maximum Information Coefficient (MIC) is designed for spatiotemporal correlation analysis, fully considering the linearity, non-linearity and non-functionality between the data of each monitoring station. The potential inherent features of the input data are effectively extracted through the convolutional neural network (CNN). Then, an optimized long short-term memroy (LSTM) network captures the short-term mutations of the time series. An attention mechanism is further designed for the proposed model, which automatically assigns different weights to different feature states at different time stages to distinguish their importance, and can achieve precise temporal and spatial interpretability. In order to further explore the long-term time features, we propose a Bi-LSTM parallel module to extract the periodic characteristics of PM concentration from both previous and posterior directions. Experimental results based on a real-world dataset indicates that the proposed model outperforms other existing state-of-the-art methods. Moreover, evaluations of recall (0.790), precision (0.848) (threshold: 151 μg/m) for 72 h prediction also verify the feasibility of our proposed model. The methodology can be used for predicting other multivariate time series data in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145082DOI Listing
May 2021

Effects of Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Lung Diseases: Current Status and Future Perspectives.

Stem Cell Rev Rep 2021 Apr 19;17(2):440-458. Epub 2020 Nov 19.

Department of Nephrology, Anhui Provincial Children's Hospital, Hefei City, Anhui Province, 230022, People's Republic of China.

Mesenchymal stromal cells (MSCs) as a kind of pluripotent adult stem cell have shown great therapeutic potential in relation to many diseases in anti-inflammation and regeneration. The results of preclinical experiments and clinical trials have demonstrated that MSC-derived secretome possesses immunoregulatory and reparative abilities and that this secretome is capable of modulating innate and adaptive immunity and reprograming the metabolism of recipient cells via paracrine mechanisms. It has been recognized that MSC-derived secretome, including soluble proteins (cytokines, chemokines, growth factors, proteases), extracellular vesicles (EVs) and organelles, plays a key role in tissue repair and regeneration in bronchopulmonary dysplasia, acute respiratory distress syndrome (ARDS), bronchial asthma, chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), pulmonary arterial hypertension, and silicosis. This review summarizes the known functions of MSC-EV modulation in lung diseases, coupled with the future challenges of MSC-EVs as a new pharmaceutical agent. The identification of underlying mechanisms for MSC-EV might provide a new direction for MSC-centered treatment in lung diseases.Graphical abstract.
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http://dx.doi.org/10.1007/s12015-020-10085-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675022PMC
April 2021

Phenolic glycosides from and their anti-inflammatory effects.

Nat Prod Res 2020 Nov 18:1-8. Epub 2020 Nov 18.

The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, State Key Laboratory, Breeding Base of Systematic Research Development and Utilization of Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Two new phenolic glycosides 7,8-threo-4,7,9,9'-tetrahydroxy-3-methoxy-8--4'-neolignan-3'--(3''-α-L-arabinofuranosyl)--D-glucopyranoside. (), 4-(4'-hydroxyphenyl)-2-butanone-4''--(6--D-xylosyl)-β--glucopyranoside (), along with two known related analogues 7,8-threo-4,7,9,9'-tetrahydroxy-3-methoxy-8--4'-neolignan-3'--β--glucopyranoside (), 4-(4'-hydroxyphenyl)-2-butanone-4'--β--glucopyranoside () were obtained from the roots of ffi. Combined with acid hydrolysis derivatization, the absolute configurations of these new compounds were elucidated by comprehensive analyses of spectroscopic data including nuclear magnetic resonance (NMR), electrospray ionization high resolution mass (HRESIMS) as well as circular dichroism (CD). Compounds - exhibited anti-inflammatory properties in vitro by attenuating the production of inflammatory mediators, such as nitric oxide (NO) as well as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).
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http://dx.doi.org/10.1080/14786419.2020.1849202DOI Listing
November 2020

Event/Self-Triggered Consensus Control of Multiagent Systems With Undesirable Sensor Signals.

IEEE Trans Cybern 2020 Oct 29;PP. Epub 2020 Oct 29.

This article focuses on event-triggered consensus control for multiagent systems subject to sensor faults or noises. First, a descriptor state observer with a low-pass filtering characteristic being developed for each agent using output information. The convergence regions of estimation errors can be reduced by a nonsingular suppression matrix. Leader-follower event-triggered consensus protocols with continuous-time communication are designed for multiagent systems based on the estimated states. By virtue of the Jordan form of the Laplacian matrix, the stability conditions are derived by using the Lyapunov analysis. Then, new self-triggered consensus protocols are designed for the multiagent systems to remove the requirement of the continuous monitoring triggering condition and continuous communication simultaneously. The triggering interval is proved greater than 0, and the Zeno behavior is excluded for all agents. Finally, numerical simulations are conducted to demonstrate the effectiveness of the proposed design.
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http://dx.doi.org/10.1109/TCYB.2020.3026215DOI Listing
October 2020

Genomes of the Banyan Tree and Pollinator Wasp Provide Insights into Fig-Wasp Coevolution.

Cell 2020 11 8;183(4):875-889.e17. Epub 2020 Oct 8.

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address:

Banyan trees are distinguished by their extraordinary aerial roots. The Ficus genus includes species that have evolved a species-specific mutualism system with wasp pollinators. We sequenced genomes of the Chinese banyan tree, F. microcarpa, and a species lacking aerial roots, F. hispida, and one wasp genome coevolving with F. microcarpa, Eupristina verticillata. Comparative analysis of the two Ficus genomes revealed dynamic karyotype variation associated with adaptive evolution. Copy number expansion of auxin-related genes from duplications and elevated auxin production are associated with aerial root development in F. microcarpa. A male-specific AGAMOUS paralog, FhAG2, was identified as a candidate gene for sex determination in F. hispida. Population genomic analyses of Ficus species revealed genomic signatures of morphological and physiological coadaptation with their pollinators involving terpenoid- and benzenoid-derived compounds. These three genomes offer insights into and genomic resources for investigating the geneses of aerial roots, monoecy and dioecy, and codiversification in a symbiotic system.
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http://dx.doi.org/10.1016/j.cell.2020.09.043DOI Listing
November 2020

A Pooled Study of Angiotensin-Converting Enzyme Insertion/Deletion Gene Polymorphism in Relation to Risk, Pathology and Prognosis of Childhood Immunoglobulin A Vasculitis Nephritis.

Biochem Genet 2021 Feb 26;59(1):202-218. Epub 2020 Sep 26.

Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, 230022, Anhui, China.

The angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism has been inconsistently reported to be a risk factor for Childhood immunoglobulin A vasculitis (IgAV) nephritis. We comprehensively searched electronic databases as of Jan 2020. Nineteen studies with 1104 cases and 1589 controls were included. Sensitivity analyses based on different subgroups were performed. Further analyses were conducted for association of ACE polymorphism with disease severity and prognosis. Significant associations were found between ACE I/D polymorphism and childhood IgAV nephritis, with the strongest association in DD vs. II comparison (OR 1.72, 95% CI 1.21-2.46). Subgroup analyses generally showed significant results. Besides, ACE polymorphism was significantly associated with proteinuria (DD + DI vs. II: OR 2.22, 95% CI 1.14-4.33; DI + II vs. DD: OR 0.49, 95% CI 0.30-0.81) and worse prognosis (the strongest effect in DD + DI vs. II: OR 4.43, 95% CI 1.84-10.71) among children with IgAV nephritis. The ACE polymorphism seemed not to be associated with hematuria, hypertension, and renal pathology. This study suggested significant association of ACE gene polymorphism with the risk of IgAV nephritis in children. D allele in the ACE genotype could be a useful genetic marker to predict proteinuria and worse prognosis for childhood IgAV nephritis.
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http://dx.doi.org/10.1007/s10528-020-09999-2DOI Listing
February 2021

Niclosamide: drug repurposing for human chondrosarcoma treatment via the caspase-dependent mitochondrial apoptotic pathway.

Am J Transl Res 2020 15;12(7):3688-3701. Epub 2020 Jul 15.

Department of Orthopedic Surgery, The Second Affiliated Hospital of Chongqing Medical University Chongqing 400010, China.

Poor sensitivity to chemotherapy drugs and high recurrence rates are the bottlenecks to successful chondrosarcoma treatment. Notably, niclosamide has been identified as a potential anti-cancer agent. To investigate the effects and mechanisms of niclosamide in the context of human chondrosarcoma treatment, SW1353 and CAL78 human chondrosarcoma cells were treated with various concentrations of niclosamide. The CKK-8 assay was performed to quantify cell viability. Cell proliferation was determined with crystal violet staining and colony forming assays. TUNEL and annexin V-FITC flow cytometry assays were performed to detect cell apoptosis. Wound healing and Transwell assays were conducted to evaluate migratory and invasive cell behaviors. The effect of niclosamide on the mitochondria was evaluated with the JC-1 and Seahorse Cell Mito Stress Assays. The expression of caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, and β-tubulin levels were investigated by western blotting. Collectively, the data demonstrated that niclosamide inhibited cell growth and proliferation, attenuated migratory and invasive cell behaviors, and promoted apoptosis. Niclosamide is as a potent chondrosarcoma tumor inhibitor that activates the caspase-dependent mitochondrial apoptotic pathway and could be a novel therapeutic approach to treat chondrosarcoma.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407720PMC
July 2020

ClC-3 induction protects against cerebral ischemia/reperfusion injury through promoting Beclin1/Vps34-mediated autophagy.

Hum Cell 2020 Oct 9;33(4):1046-1055. Epub 2020 Aug 9.

Department of Neurology, The First Teaching Hospital of Jilin University, No. 71 Xinmin Street, Chaoyang District, Changchun, 130021, Jilin Province, People's Republic of China.

Acute ischemic stroke is a devastating disease with very limited therapeutics. Growing appreciation of dysregulated autophagy contributes to the progression of brain ischemic injury, making it to be an appealing intervention target. In terms of its well-characterized consequences, the signal molecules required for autophagy activation are rather poorly defined. Here, we found the induction of chloride channel-3 (ClC-3) directly activated autophagy, which played an important role in limiting cerebral ischemia/reperfusion (I/R) injury. Further mechanism exploration discovered that the up-regulation of ClC-3 was critical for the interaction of Beclin1 and Vps34. After ClC-3 knockdown using adeno-associated virus vectors in vivo, the autophagy activation was partially inhibited through disrupting the formation of Beclin1 and Vps34 complex. Consistent with these observations, ClC-3 knockdown could also significantly aggravated cerebral I/R injury through suppressing autophagy in vivo, which further confirmed the neuroprotective roles of ClC-3. Collectively, we provided an novel evidence for ClC-3 serving as a crucial regulator of autophagy; and our results indicated that the induction of ClC-3 may serve as a self-protective mechanism against cerebral I/R injury.
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http://dx.doi.org/10.1007/s13577-020-00406-xDOI Listing
October 2020

Discriminant Geometrical and Statistical Alignment With Density Peaks for Domain Adaptation.

IEEE Trans Cybern 2020 Jun 11;PP. Epub 2020 Jun 11.

Unsupervised domain adaptation (DA) aims to perform classification tasks on the target domain by leveraging rich labeled data in the existing source domain. The key insight of DA is to reduce domain divergence by learning domain-invariant features or transferable instances. Despite its rapid development, there still exist several challenges to explore. At the feature level, aligning both domains only in a single way (i.e., geometrical or statistical) has limited ability to reduce the domain divergence. At the instance level, interfering instances often obstruct learning a discriminant subspace when performing the geometrical alignment. At the classifier level, only minimizing the empirical risk on the source domain may result in a negative transfer. To tackle these challenges, this article proposes a novel DA method, called discriminant geometrical and statistical alignment (DGSA). DGSA first aligns the geometrical structure of both domains by projecting original space into a Grassmann manifold, then matches the statistical distributions of both domains by minimizing their maximum mean discrepancy on the manifold. In the former step, DGSA only selects the density peaks to learn the Grassmann manifold and so to reduce the influences of interfering instances. In addition, DGSA exploits the high-confidence soft labels of target landmarks to learn a more discriminant manifold. In the latter step, a structural risk minimization (SRM) classifier is learned to match the distributions (both marginal and conditional) and predict the target labels at the same time. Extensive experiments on objection recognition and human activity recognition tasks demonstrate that DGSA can achieve better performance than the comparison methods.
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http://dx.doi.org/10.1109/TCYB.2020.2994875DOI Listing
June 2020

Coexisting overexpression of STOML1 and STOML2 proteins may be associated with pathology of oral squamous cell carcinoma.

Oral Surg Oral Med Oral Pathol Oral Radiol 2020 Jun 8;129(6):591-599.e3. Epub 2020 May 8.

Center for Research and Technology of Precision Medicine, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China. Electronic address:

Objective: The present study aimed to investigate the expression and co-localization of stomatin-like protein-1 (STOML1) and stomatin-like protein-2 (STOML2) in oral squamous cell carcinoma (OSCC) tissues in situ and evaluate their pathologic roles in OSCC.

Study Design: STOML1 and STOML2 in human OSCC tissues (n = 109) and normal oral/paracancerous tissues (n = 19) were detected by using multiple immunohistochemistry (IHC) staining. Positive staining scores and clinicopathologic features during the OSCC process were analyzed.

Results: STOML1 and STOML2 were significantly overexpressed in OSCC tissues compared with normal oral tissue/paracancerous tissues (P < .0001 and P < .0001, respectively). Furthermore, both STOML1 and STOML2 were positively associated with pathologic tumor (T) stages. Positive signals of both STOML1 and STOML2 were mainly localized to the cell membrane and the cytoplasm, whereas those of STOML1 were also expressed in the cell nucleus.

Conclusions: Our results indicated that overexpression of STOML1 and STOML2 was significantly associated with T1 and T2 stages of OSCC. STOML1 and STOML2 were mainly co-localized at the cell membrane and the cytoplasm. These findings suggested that either STOML1 or STOML2 may play critical roles in OSCC development and may serve as potential diagnostic biomarkers and therapeutic targets.
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http://dx.doi.org/10.1016/j.oooo.2020.01.011DOI Listing
June 2020

Synthesis and Biological Evaluation of HDAC Inhibitors With a Novel Zinc Binding Group.

Front Chem 2020 15;8:256. Epub 2020 Apr 15.

College of Pharmacy, Hebei Medical University, Shijiazhuang, China.

Vorinostat (SAHA) with great therapeutic potential has been approved by the FDA for the treatment of cutaneous T-cell lymphoma as the first HDACs inhibitor, but the drawbacks associated with hydroxamic acid group (poor stability, easy metabolism, weak binding ability to class IIa isozymes, and poor selectivity) have been exposed during the continuous clinical application. Based on the pharmacophore of HDAC inhibitors, two series of compounds with novel zinc binding group (ZBG) were designed and synthesized, and the antitumor bioactivities were evaluated in four human cancer cell lines (A549, Hela, HepG2, and MCF-7). Among the synthesized compounds, compounds , , , , and exhibited promising inhibitory activities against the selected tumor cell lines, especially compounds and on Hela's cytostatic activity (: IC = 11.15 ± 3.24 μM; : IC = 13.68 ± 1.31 μM). The enzyme inhibition assay against Hela extracts and HDAC1&6 subtypes showed that compound had a certain broad-spectrum inhibitory activity, while compound had selective inhibitory activity against HDAC6, which was consistent with Western blot results. In addition, the inhibitory mechanism of compounds and in HDAC1&6 were both compared through computational approaches, and the binding interactions between the compounds and the enzymes target were analyzed from the perspective of energy profile and conformation. In summary, the compounds with novel ZBG exhibited certain antitumor activities, providing valuable hints for the discovery of novel HDAC inhibitors.
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http://dx.doi.org/10.3389/fchem.2020.00256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174758PMC
April 2020

Unprecedented Quassinoids from : Biogenetic Evidence and Antifeedant Effects.

J Nat Prod 2020 05 20;83(5):1674-1683. Epub 2020 Apr 20.

Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, People's Republic of China.

Six new quassinoids (-) were isolated from the roots of , and their structures with absolute configurations were determined unambiguously by spectroscopic analyses and single-crystal X-ray crystallographic experiments. Compounds and are the first members of a new class of quassinoids with an unusual C carbon skeleton. Compound features a C cage-like scaffold with an unprecedented densely functionalized 2,5-dioxatricyclo[5.2.2.0]undecane core. The discovery of the two C quassinoids and has provided firm evidence for the better understanding the biogenetic process from C triterpenoid precursors to quassinoids. Compound exhibited significant antifeedant activity on the diamondback moth (DBM) larvae and excellent systemic absorption and accumulated properties in .
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http://dx.doi.org/10.1021/acs.jnatprod.0c00244DOI Listing
May 2020

Long-Range Binocular Vision Target Geolocation Using Handheld Electronic Devices in Outdoor Environment.

IEEE Trans Image Process 2020 Apr 7. Epub 2020 Apr 7.

Binocular vision is a passive method of simulating the human visual principle to perceive the distance to a target. Traditional binocular vision applied to target localization is usually suitable for short-range area and indoor environment. This paper presents a novel vision-based geolocation method for long-range targets in outdoor environment, using handheld electronic devices such as smart phones and tablets. This method solves the problems in long-range localization and determining geographic coordinates of the targets in outdoor environment. It is noted that these sensors necessary for binocular vision geolocation such as the camera, GPS, and inertial measurement unit (IMU), are intergrated in these handheld electronic devices. This method, employing binocular localization model and coordinate transformations, is provided for these handheld electronic devices to obtain the GPS coordinates of the targets. Finally, two types of handheld electronic devices are used to conduct the experiments for targets in long range up to 500m. The experimental results show that this method yields the target geolocation accuracy along horizontal direction with nearly 20m, achieving comparable or even better performance than monocular vision methods.
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http://dx.doi.org/10.1109/TIP.2020.2984898DOI Listing
April 2020

Fatal congenital tuberculosis owing to late diagnosis of maternal tuberculosis: case report and review of congenital tuberculosis in China.

Paediatr Int Child Health 2020 08 20;40(3):194-198. Epub 2020 Mar 20.

Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University , Anhui, China.

Congenital tuberculosis (TB) is rare and the prognosis is poor if not detected early. The diagnosis is often delayed owing to non-specific clinical presentation, misdiagnosis and undiagnosed maternal TB during pregnancy. A 12-day-old girl presented with a 5-day history of fever, cough, poor feeding and respiratory distress. Her mother had a cough and fever at 30 weeks gestation which was managed empirically as community-acquired pneumonia without a TB workup. Immediately postpartum, her mother developed a high fever and shortness of breath and required admission to the intensive care unit. The infant was separated from her mother after delivery. The infant's chest radiograph showed bilateral miliary nodules. Thoracic and abdominal computed tomography (CT) showed multiple enlarged lymph nodes and congenital TB was suspected. Early morning gastric aspirate and sputum (obtained through a suction tube) were positive for acid-fast bacilli on smear microscopy and subsequently was cultured from both specimens. Lumbar puncture was performed and cerebrospinal fluid (CSF) was compatible with TB meningitis. TB-polymerase chain reaction (TB-PCR) was positive. Her mother was diagnosed with miliary TB on postpartum day 17. Both were given anti-TB chemotherapy. Unfortunately, despite the treatment, the infant died from multiple organ dysfunction syndrome (MODS) caused by congenital TB at the age of 14 days. This case highlights the importance of screening pregnant women for TB in regions where it is highly prevalent. A high index of suspicion of maternal and congenital TB is critical to early diagnosis, especially in such regions.
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http://dx.doi.org/10.1080/20469047.2020.1743932DOI Listing
August 2020

Inwardly rectifying potassium channel 5.1: Structure, function, and possible roles in diseases.

Genes Dis 2021 May 21;8(3):272-278. Epub 2020 Mar 21.

Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, PR China.

Inwardly rectifying potassium (Kir) channels make it easier for K to enter into a cell and subsequently regulate cellular biological functions. Kir5.1 (encoded by ) alone can form a homotetramer and can form heterotetramers with Kir4.1 (encoded by ) or Kir4.2 (encoded by ). In most cases, homomeric Kir5.1 is non-functional, while heteromeric Kir5.1 on the cell membrane contributes to the inward flow of K ions, which can be regulated by intracellular pH and a variety of signaling mechanisms. In the form of a heterotetramer, Kir5.1 regulates Kir4.1/4.2 activity and is involved in the maintenance of nephron function. Actually, homomeric Kir5.1 may also play a very important role in diseases, including in the ventilatory response to hypoxia and hypercapnia, hearing impairment, cardiovascular disease and cancer. With an increase in the number of studies into the roles of Kir channels, researchers are paying more attention to the pathophysiological functions of Kir5.1. This minireview provides an overview regarding these Kir5.1 roles.
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http://dx.doi.org/10.1016/j.gendis.2020.03.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093645PMC
May 2021

Overexpression of IL-8 promotes cell migration via PI3K-Akt signaling pathway and EMT in triple-negative breast cancer.

Pathol Res Pract 2020 Apr 26;216(4):152902. Epub 2020 Feb 26.

Department of Laboratory Medicine, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong, Chongqing, 400016, PR China. Electronic address:

Triple-negative breast cancer (TNBC) is a type of malignant and heterogeneous tumor in premenopausal females with ineffective therapeutic targets. IL-8 is one of the earliest discovered chemotaxis cytokines which expression is closely related to the progress of various cancers. Previous studies showed that IL-8 determines the prognosis of TNBC patients, nevertheless how IL-8 influences the progress of TNBC is unclear. In our studies, we discovered that overexpression of IL-8 promotes TNBC cells (TNBCs) migration and tumor growth via the PI3K-Akt and MAPK signaling pathway. Cell-cycle of TNBCs arrest at S phase by overexpression of IL-8, however, there is no significant variation on the cell viability and cell apoptosis of TNBCs. Besides, overexpression of IL-8 result in the downregulation of E-cadherin and the upregulation of Cyclin B1 in MDA-MB-231 cells. Taken together, our results suggest that IL-8 performs a crucial role in the progress of TNBC, and it could be a novel therapeutic target of TNBC.
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http://dx.doi.org/10.1016/j.prp.2020.152902DOI Listing
April 2020

Prognostic value of miR-21 in gliomas: comprehensive study based on meta-analysis and TCGA dataset validation.

Sci Rep 2020 03 6;10(1):4220. Epub 2020 Mar 6.

Department of Epidemiology and Health Statistics, Xiangya Public Health School, Central South University, Changsha, 410078, Hunan, P.R. China.

Recent studies have highlighted the value of microRNA-21 (miR-21) as a prognostic biomarker in gliomas. However, the role of miR-21 in predicting prognosis remains controversial. We performed a comprehensive study based upon a meta-analysis and The Cancer Genome Atlas (TCGA) glioma dataset validation to clarify the prognostic significance of miR-21 in glioma patients. In this study, we searched Embase, PubMed, Web of science, CNKI, SinoMed, and Wanfang databases for records up to May 2018. Relevant data were extracted to assess the correlation between miR-21 expression and survival in glioma patients. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were used to describe association strength. We further used multivariate Cox regression analysis to assess miR-21 expression in the TCGA glioma dataset to validate the relationship between miR-21 expression and survival. Nine studies were included in the meta-analysis. Among them, eight studies provided data on overall survival (OS) with a pooled HR of 1.91 (95% CI: 1.34, 2.73), indicating that higher expression of miR-21 was significantly associated with worse OS in glioma patients; for the other study, which provided data on progression-free survival (PFS), no statistically significant HR was reported for PFS in the glioma patients (HR = 1.23, 95% CI: 0.41, 3.72). A multivariate Cox regression analysis of the miR-21 expression in the TCGA glioma dataset revealed that overexpression of miR-21 was a potential independent prognostic biomarker of poorer OS (HR = 1.27, 95% CI: 1.01, 1.59) and poorer PFS (HR = 1.46, 95% CI: 1.17, 1.82). Our findings suggest that higher expression of miR-21 is correlated with poorer glioma prognosis.
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http://dx.doi.org/10.1038/s41598-020-61155-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060265PMC
March 2020

Compromised Dynamic Cerebral Autoregulation in Patients With Idiopathic Rapid Eye Movement Behavior Disorder: A Case-Control Study Using Transcranial Doppler.

Front Psychiatry 2020 19;11:51. Epub 2020 Feb 19.

Department of Neurology, The First Hospital of Jilin University, Changchun, China.

Background: Patients with idiopathic rapid eye movement behavior disorder (IRBD) have been suggested to exhibit altered cerebral perfusion and abnormal cerebral blood flow, which imply a possibility of cerebral autoregulation (CA) impairment. We aimed to investigate the dynamic CA (dCA) in patients with IRBD during wakefulness and to explore the correlations between dCA parameters and clinical measurements.

Methods: We assessed the dCA capability of 30 patients with IRBD and 36 sex- and age-matched healthy controls by using transcranial Doppler and finger plethysmography. CA function was evaluated by transfer function analysis based on spontaneous oscillation of cerebral blood flow and arterial blood pressure. Transfer function parameters (phase difference and gain) were used to quantify the CA.

Results: No significant differences were observed between the right and left middle cerebral artery dCA parameters (phase difference and gain) of both groups. Patients with IRBD had significantly lower phase difference than the healthy controls, indicating their impaired CA capability. Besides, the value of gain in patients with IRBD was higher than the healthy controls, but the difference did not reach statistical level.

Conclusions: CA function is compromised in patients with IRBD during wakefulness, which might be an intermediate link between IRBD and neurological symptoms.
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http://dx.doi.org/10.3389/fpsyt.2020.00051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042385PMC
February 2020