Publications by authors named "Fan Wu"

1,152 Publications

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Homochiral Ferromagnetic Coupling Dy Single-Molecule Magnets with Strong Magneto-Optical Faraday Effects at Room Temperature.

Inorg Chem 2021 Aug 4. Epub 2021 Aug 4.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

By the bridging action of the 6-chloro-2-hydroxypyridine (Hchp) ligand and the terminal coordination role of the homochiral ligand, (-)/(+)-3-trifluoroacetyl camphor (l-Htfc/d-Htfc), a pair of enantiomerically pure dysprosium(III) dinuclear complexes, [Dy(l-tfc)(chp)(MeOH)] (l-) and [Dy(d-tfc)(chp)(MeOH)] (d-), was obtained. Their circular dichroism (CD) spectra verified their enantiomeric nature. Magnetic investigation indicated that they exhibit ferromagnetic interaction and good zero field single-molecule magnet (SMM) properties. The / values of l- and d- at 0 Oe are 180.5 and 181.3 K, respectively, which are large values for homochiral Dy(III) SMMs. A reasonable explanation for the magnetic properties of l- and d- was supplied by calculations. Remarkably, magnetic circular dichroism (MCD) investigation revealed that the chiral Dy enantiomers show a strong magneto-optical Faraday effect at room temperature, suggesting potential applications in magneto-optical devices.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01218DOI Listing
August 2021

Revealing ammonia quantification minefield in photo/electrocatalysis.

Angew Chem Int Ed Engl 2021 Jul 30. Epub 2021 Jul 30.

Technical Institute of Physics and Chemistry (TIPC), Chinese Academy of Sciences (CAS), Key Laboratory of Photochemical Conversion and Optoelectronic Materials, No.29 Zhongguancun East Road, Haidian District,, 100190, Beijing, CHINA.

Photo/electrocatalytic ammonia synthesis-a low-carbon and sustainable process has developed fast over the past decade while the ammonia yields over state-of-the-art photo/electrocatalysts are still very moderate. Such low concentration of synthesized NH 3 brings about a challenge to the reliable quantification of the product in photo/electrocatalysis. Notably, we found that the quantitative detection of ammonia concentration below 0.2 ppm is error-prone, which is likely the case happening in the majority of photo/electrocatalytic NH 3 synthesis, thus arising concerns about the rationality and accuracy for low-concentration ammonia quantification in these processes. Herein, we discuss the methodology used and analyze the reliability of various detection methods for the detection of trace ammonia in aqueous media. By regulating the parameters of detection methods, the experimental detection limitation can be expanded from 0.2 ppm to 0.1 ppm, even lower. The challenges facing the detection of low concentration of ammonia in photo/electrocatalysis can be overcome by integration with multiple detection methods. According to the data presented, we also propose an effective criterion for precise quantification of ammonia, avoiding the unreasonable comparisons in photo/electrocatalytic ammonia synthesis.
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http://dx.doi.org/10.1002/anie.202108769DOI Listing
July 2021

NIR-triggered intracellular H+ transients for lamellipodia-collapsed antimetastasis and enhanced chemodynamic therapy.

Angew Chem Int Ed Engl 2021 Jul 28. Epub 2021 Jul 28.

Fudan University, Department of Materials Science, 220 Handan Road, Shanghai 200433, P.R. China, 200433, Shanghai, CHINA.

In solid tumours, tumour invasion and metastasis account for 90% of cancer-related deaths. Cell migration is steered by the lamellipodia formed at the leading edge. These lamellipodia can drive the cell body forward via its mechanical deformation regulated by cofilin. Inhibiting cofilin activity can cause significant defects in directional lamellipodia formation and the locomotory capacity of cell invasion, thus contributing to antimetastatic treatment. Here, a near infrared light (NIR)-controlled nanoscale proton supplier was designed with upconversion nanoparticles (UCNPs) with a core coated in MIL-88B for interior photoacids loading; this photoacids loading can boost H+ transients in cells, which converts the cofilin to an inactive form. Strikingly, inactive cofilin loses the ability to mediate lamellipodia deformation for cell migration. Additionally, the iron, which serves as a catalytic active centre in MIL-88B, initiates an enhanced Fenton reaction due to the increased H+ in the tumour, ultimately achieving intensive chemodynamic therapy (CDT). This work provides new insight into H+ transients in cells, which not only regulates cofilin protonation for antimetastatic treatment but also improves chemodynamic therapy.
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http://dx.doi.org/10.1002/anie.202107588DOI Listing
July 2021

Baicalin Alleviates Oxidative Stress and Inflammation in Diabetic Nephropathy via Nrf2 and MAPK Signaling Pathway.

Drug Des Devel Ther 2021 21;15:3207-3221. Epub 2021 Jul 21.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.

Background: Oxidative stress and inflammation play essential roles in the development and progression of diabetic nephropathy (DN). Baicalin (BAI), a natural flavonoid, has been showed to have a renoprotective effect in various renal diseases. However, its underlying mechanisms in DN remain unclear. In this study, we explored the potential effects and underlying mechanisms of BAI on DN using a spontaneous DN model.

Methods: The protective effects of BAI on DN have been evaluated by detecting DN-related biochemical indicators, kidney histopathology and cell apoptosis. After that, we examined the level of renal oxidative stress and inflammation to explain BAI's renoprotective effects. Then, Nrf2 pathway was tested to clarify its antioxidant activity, and kidney transcriptomics was conducted to elucidate its anti-inflammatory activity. Finally, Western blot was applied for final mechanism verification.

Results: Our results found that BAI effectively ameliorated diabetic conditions, proteinuria, renal histopathological changes and cell apoptosis in DN. BAI significantly improved the kidney levels of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and catalase (CAT), and reduced malondialdehyde (MDA) level. Meanwhile, the infiltration of inflammatory cells including T-lymphocytes, T-helper cells, neutrophils and macrophages, and the mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, MCP-1 and TNFα) were also obviously inhibited by BAI. Afterward, Western blot found that BAI significantly activated Nrf2 signaling and increased the expression of downstream antioxidant enzymes (HO-1, NQO-1). Kidney transcriptomics revealed that the inhibition of MAPK signaling pathway may contribute to BAI's anti-inflammatory activity, which has also been verified in later experiment. BAI treatment did obviously inhibit the activation of canonical pro-inflammatory signaling pathway MAPK family, such as Erk1/2, JNK and P38.

Conclusion: In summary, our data demonstrated that BAI can treat DN by alleviating oxidative stress and inflammation, and its underlying mechanisms were associated with the activation of Nrf2-mediated antioxidant signaling pathway and the inhibition of MAPK-mediated inflammatory signaling pathway.
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http://dx.doi.org/10.2147/DDDT.S319260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313380PMC
July 2021

Characteristics of in-situ hydrogen biomethanation at mesophilic and thermophilic temperatures.

Bioresour Technol 2021 Oct 24;337:125455. Epub 2021 Jun 24.

State Key Laboratory of Heavy Oil Processing, Beijing Key Laboratory of Biogas Upgrading Utilization, College of New Energy and Materials, China University of Petroleum Beijing (CUPB), Beijing 102249, PR China. Electronic address:

In-situ hydrogen biomethanation is a promising technology to upgrade biogas. The efficiency of biomethanation relies on various parameters, e.g. gas supplement, temperature and hydrogenotrophic methanogens. Therefore, it is important to investigate the characteristics of in-situ hydrogen biomethanation under different conditions. In this study, two experiments (lasted for 91 days and 105 days) were carried out to investigate the impacts of feeding gas and operating conditions on performances of reactors and microorganisms. During the whole experiment, no obvious fluctuation of pH and limitation of gas-liquid mass transfer were found. Results showed that the hydrogenotrophic methanogenesis performed better at thermophilic condition, while the dominant archaea genera at mesophilic and thermophilic temperature was determined to be Methanobacterium and Methanothermobacter, respectively. The highest CH content (greater than 90%) was obtained when H and CO was feeding at ratio of 4:1 and Methanothermobacter was dominant. These findings can provide useful information for promoting hydrogen biomethanation.
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http://dx.doi.org/10.1016/j.biortech.2021.125455DOI Listing
October 2021

Social isolation and health-promoting behaviors among older adults living with different health statuses: A cross-sectional study.

Authors:
Fan Wu Yu Sheng

Int J Nurs Sci 2021 Jul 3;8(3):304-309. Epub 2021 Jun 3.

School of Nursing, Peking Union Medical College, Beijing, China.

Objectives: To describe the characteristics and relationships of social isolation and health promoting behaviors of Chinese older adults with different health statuses.

Methods: Totally 485 older adults were recruited. They were divided into three groups based on their health status: healthy group ( = 72), group with one disease ( = 183) and group with multimorbidity ( = 230). Lubben Social Network Scale-6 and Health-Promoting Lifestyle Profile II were used to measure their social isolation and health-promoting behaviors, respectively.

Results: Among the aged with one disease or multimorbidity, 30.6% (56/183) and 31.7% (73/230) were socially isolated, respectively, based on the score of the Lubben Social Network Scale-6. The three groups differed significantly in health-promoting behaviors and scores on several subscales. The group with multimorbidity exhibited more social isolation and fewer health-promoting behaviors than other groups. Social isolation was among the factors adversely affecting health-promoting behaviors in older adults.

Conclusions: Health care providers should help older adults with multimorbidity preserve the remaining abilities to improve health-promoting behaviors. Meanwhile, it is important to support healthy older adults to increase self-responsibility to achieve healthy aging.
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http://dx.doi.org/10.1016/j.ijnss.2021.05.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283714PMC
July 2021

Electrochemical Radical δ-H Sulfonylation Reaction for the Synthesis of 4-((Aryl,Arylsul fonyl)methylene)-2,5-Cyclohexadiene Derivatives.

J Org Chem 2021 Jul 22. Epub 2021 Jul 22.

Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People's Republic of China.

A novel and efficient electrochemical radical δ-H sulfonylation reaction of -quinone methides (-QMs) and sodium sulfinates has been achieved under common laboratory conditions. In this strategy, a new C(sp)-S bond was constructed for the synthesis of 4-((aryl,arylsulfonyl)methylene)-2,5-cyclohexadiene derivatives with a broad substrate scope, good functional group tolerance, and mild conditions. Further studies showed that the reaction had an excellent regional selectivity.
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http://dx.doi.org/10.1021/acs.joc.1c01213DOI Listing
July 2021

A new RHD variant allele caused by an RHD c.1228-1G>C mutation in a Chinese family.

Transfusion 2021 Jul 22. Epub 2021 Jul 22.

Department of Blood Transfusion, The First Affiliated Hospital of Shenzhen University School of Medicine, The Second People's Hospital of Shenzhen, Shenzhen, China.

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http://dx.doi.org/10.1111/trf.16510DOI Listing
July 2021

The role mA RNA methylation is CNS development and glioma pathogenesis.

Mol Brain 2021 Jul 19;14(1):119. Epub 2021 Jul 19.

Department of Neurosurgery, Cancer Hospital of University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, 310022, People's Republic of China.

Epigenetic abnormalities play a crucial role in many tumors, including glioma. RNA methylation occurs as an epigenetic modification similar to DNA methylation and histone modification. mA methylation is the most common and most intensively studied RNA methylation, which can be found throughout the RNA life cycle and exert biological functions by affecting RNA metabolism. The mA modification is primarily associated with three types of protease, which are encoded by the writer, eraser and reader genes, respectively. It has been shown that the mA methylation has close connections with the occurrence and development of many tumors, including glioma. In this study, the concept and the research progress of mA methylation are reviewed, especially the role of mA methylation in glioma. Moreover, we will discuss how glioma is paving the way to the development of new therapeutic options based on the inhibition of mA deposition.
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http://dx.doi.org/10.1186/s13041-021-00831-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290532PMC
July 2021

The Effects of Overhang Forming Direction on Thermal Behaviors during Additive Manufacturing Ti-6Al-4V Alloy.

Materials (Basel) 2021 Jul 5;14(13). Epub 2021 Jul 5.

Tech Institute for Advanced Material, Nanjing Tech University, Nanjing 210009, China.

Selective laser melting was recently introduced to fabricate complex parts that are likely to contain overhangs. Process parameters, scanning strategies, support structures, and fast prediction techniques are being frequently studied, but little information about overhang forming direction has been reported. In this study, the effects of overhang forming direction in the working plane on temperature evolution and distortion processes during selective laser melting of Ti-6Al-4V alloy were examined by means of numerical simulation and experimental verification. We found that forming from different directions can lead to significant differences in the early stage of the overhang building process, which were verified by both the simulations and the experiment. Some analyses were performed when enough layers had been built and suggestions are also given.
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http://dx.doi.org/10.3390/ma14133749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269800PMC
July 2021

Mitigation of acidogenic product inhibition and elevated mass transfer by biochar during anaerobic digestion of food waste.

Bioresour Technol 2021 Oct 13;338:125531. Epub 2021 Jul 13.

Hunan International Scientific and Technological Cooperation Base of Agricultural Typical Pollution Remediation and Wetland Protection, College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China. Electronic address:

Anaerobic digestion (AD) of food waste is widely accepted as a promising technology for both waste disposal and resource recovery. With the advancing of AD technology, to exploit the capacity of organic waste for maximum energy/resource recovery becomes the new focus and hence, improve the viability of this technology for practical application. Product inhibition and mass transfer are the common limitations encountered during AD of putrescible organic waste. Biochar materials have been widely used to promote AD process in recent years. This review summarizes the mechanism and regulation strategies of biochar and its modified derivatives in promoting AD of solid waste (mainly food waste) from the three aspects of hydrolysis, syntrophic acetogenesis, and methane production. At the same time, the relationship between carbon materials and electron transfer among anaerobic microbes is summarized from the perspective of microbial community. In addition, the market application of this technology was evaluated.
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http://dx.doi.org/10.1016/j.biortech.2021.125531DOI Listing
October 2021

A meta-analysis of risk factors for lymph node posterior to the right recurrent laryngeal nerve metastasis in papillary thyroid carcinoma.

Gland Surg 2021 Jun;10(6):1841-1851

Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Whether the lymph node posterior to the right recurrent laryngeal nerve (LN-prRLN) should be dissected is still controversial. This meta-analysis aimed to assess the risk factors for LN-prRLN metastasis in papillary thyroid carcinoma (PTC).

Methods: We retrieved relevant studies published before May 2020 from the Wanfang Data, CNKI, PubMed, Embase, Web of Science and Cochrane Library databases. Heterogeneity was assessed with the Q-test and inconsistency index and sensitivity analysis and subgroup analysis were then used to find the source of heterogeneity. Begg test and generate a funnel chart to assess publication bias.

Results: We retrieved 236 articles, 14 articles were selected as the subjects of our research. Fourteen studies involving 10,580 patients were analysed in this study. The LN-prRLN metastasis rate was 9.22% (975/10,580). The results of the meta-analysis showed that sex (P<0.001), age (P<0.001), tumour size (P<0.001), multifocality (P<0.001), capsular invasion (P=0.04), extrathyroidal extension (P<0.001), superficial central lymph node (VIa-LN) metastasis (P<0.001), and lateral lymph node (LLN) metastasis (P<0.001) were correlated with LN-prRLN metastasis.

Discussion: Male sex, age ≤45 years, tumour size >1 cm, multifocality, capsular invasion or extrathyroidal extension, and VIa-LN metastasis or LLN metastasis in PTC patients were significant risk factors for predicting LN-prRLN metastasis. B ultrasound and CT scans are expected to predict LN-prRLN metastasis in the future.

Registration: This research is registered on the PROSPERO website (registration number: CRD42020200898).
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http://dx.doi.org/10.21037/gs-21-177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258886PMC
June 2021

[Enteral feeding in extremely low birth weight infants with a gestational age of <28 weeks versus ≥ 28 weeks].

Authors:
Ying Li Fan Wu

Zhongguo Dang Dai Er Ke Za Zhi 2021 Jul;23(7):690-695

Department of Neonatology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China.

Objective: To investigate the condition of enteral feeding and the incidence of feeding intolerance (FI) in extremely low birth weight (ELBW) infants with a gestational age (GA) of < 28 weeks versus ≥ 28 weeks.

Methods: A retrospective analysis was performed for the ELBW infants who were admitted to the Department of Neonatology, The Third Affiliated Hospital of Guangzhou Medical University, from January 2018 to January 2020. According to GA, they were divided into two groups:GA < 28 weeks (=41) and GA ≥ 28 weeks (=38). The two groups were compared in terms of enteral feeding and incidence rate of FI during hospitalization.

Results: Among the 41 infants in the GA < 28 weeks group, 2 (5%) were small-for-gestational-age (SGA) infants, while among the 38 infants in the GA ≥ 28 weeks group, 24 (63%) were SGA infants. Compared with the GA ≥ 28 weeks group, the GA < 28 weeks group had significantly longer initial and final time to full enteral feeding ( < 0.05). The GA < 28 weeks group had a significantly higher recurrence rate of FI than the GA ≥ 28 weeks group (54% 32%, < 0.05). Compared with the GA < 28 weeks group, the GA ≥ 28 weeks group had a significantly lower incidence rate of FI within 1-2 weeks after birth (5% 29%, < 0.05) and a significantly shorter duration of FI ( < 0.05). The ELBW infants in the GA ≥ 28 weeks group had a higher velocity of weight gain, a shorter length of hospital stay, and a lower incidence rate of bronchopulmonary dysplasia, but a higher incidence rate of extrauterine growth retardation (EUGR) than the GA < 28 weeks group at discharge ( < 0.05) Conclusions: Compared with the ELBW infants with a GA of < 28 weeks, the ELBW infants with a GA of ≥ 28 weeks have a lower recurrence rate of FI and can achieve full enteral feeding earlier, but with a higher incidence rate of EUGR, suggesting that enteral feeding needs to be more active for the ELBW infants with a GA of ≥ 28 weeks.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292661PMC
July 2021

Structural insights into hormone recognition by the human glucose-dependent insulinotropic polypeptide receptor.

Elife 2021 Jul 13;10. Epub 2021 Jul 13.

School of Pharmacy, Fudan University, Shanghai, China.

Glucose-dependent insulinotropic polypeptide (GIP) is a peptide hormone that exerts crucial metabolic functions by binding and activating its cognate receptor, GIPR. As an important therapeutic target, GIPR has been subjected to intensive structural studies without success. Here, we report the cryo-EM structure of the human GIPR in complex with GIP and a G heterotrimer at a global resolution of 2.9 Å. GIP adopts a single straight helix with its N terminus dipped into the receptor transmembrane domain (TMD), while the C terminus is closely associated with the extracellular domain and extracellular loop 1. GIPR employs conserved residues in the lower half of the TMD pocket to recognize the common segments shared by GIP homologous peptides, while uses non-conserved residues in the upper half of the TMD pocket to interact with residues specific for GIP. These results provide a structural framework of hormone recognition and GIPR activation.
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http://dx.doi.org/10.7554/eLife.68719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298097PMC
July 2021

Low-level laser irradiation enhances the proliferation and osteogenic differentiation of PDLSCs via BMP signaling.

Lasers Med Sci 2021 Jul 10. Epub 2021 Jul 10.

Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, 145 West Changle Road, Xi'an, 710032, Shaanxi, China.

The aim of this in vitro study was to evaluate the effects of low-level laser therapy (LLLT) at different energy intensities on proliferation and osteogenesis of periodontal ligament stem cells (PDLSCs). We designed one control group, without irradiation and four testing groups, treated with LLLT (Nd:YAG;1064 nm) at 2, 4, 6, and 8 J/cm for human PDLSCs. Cell proliferation was measured using colony-forming unit fibroblast assay and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay. Osteogenic capacity of cells was determined by alkaline phosphatase (ALP) staining, ALP activity assay, Alizarin Red S staining, and the gene levels of runt-related transcription factor 2 (Runx2), ALP, osteocalcin, and bone morphogenetic protein 2 (BMP2). The effects of LLLT on secretion of TNF-α and IL-1β in PDLSCs were measured by enzyme-linked immunosorbent assay. BMP/Smad pathway was measured through the expression of Smad1/5/8 phosphorylation (P-Smad1/5/8). LDN-193189, an inhibitor of the BMP/Smad pathway, was used to explore the underlying effects of BMP/Smad signaling on the process of LLLT regulating the proliferation and osteogenesis of PDLSCs. Our results demonstrated LLLT could promote the proliferation and osteogenesis of PDLSCs at 2-6 J/cm and LLLT at 8 J/cm significantly suppress osteogenic differentiation of PDLSCs. Moreover, LLLT stimulated the secretion of TNFα and IL-β1. Finally, we found the irradiation positively modulates the P-Smad1/5/8 level. When the cells were treated with LDN-193189, the proliferation and osteogenic effects of LLLT on PDLSCs were attenuated. In conclusion, LLLT may upregulate the proliferation and bone formation ability of PDLSCs via the BMP/Smad signaling.
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http://dx.doi.org/10.1007/s10103-021-03338-6DOI Listing
July 2021

The spatial distribution of the photosynthetic picoeukaryotes community structure in Lake Hongze.

FEMS Microbiol Ecol 2021 07;97(8)

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, 73 East Beijing Road, Nanjing 210008, China.

The spatial dynamics of picophytoplankton were investigated by flow cytometry and high-throughput sequencing in Lake Hongze, a large river-connecting lake. Picophytoplankton were mainly composed of phycocyanin-rich picocyanobacteria (PCY) and photosynthetic picoeukaryotes (PPEs). Picocyanobacteria was the dominant picophytoplankton group since the contribution of PPEs to total picophytoplankton was only 11.78%. However, PPEs were highly diverse and were composed of Chlorophyta, Bacillariophyta and Chrysophyceae. Environmental factors showed spatial differences, particularly in total phosphorus (TP), suspended solids (SS) and chemical oxygen demand (CODMn), which showed relatively high concentrations around the river channel. The abundances of PPEs and PCY showed similar spatial patterns, which were relatively low in the river course since they were negatively related to SS and CODMn. The top 10 OTUs contributed 79.18% of the total PPEs reads and affiliated with main species in PPEs. CCA results showed that, PPEs community composition was significantly impacted by temperature and DO at sites away from river channel, while was only influenced by nitrite at sites around the river channel.
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http://dx.doi.org/10.1093/femsec/fiab100DOI Listing
July 2021

HtrA serine proteases in cancers: A target of interest for cancer therapy.

Biomed Pharmacother 2021 Jul 8;139:111603. Epub 2021 May 8.

Hepatobiliary/Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Transplantation, Chinese Academy of Medical Sciences, Nanjing, China. Electronic address:

The HtrA protein family is composed by evolutionally-conserved serine proteases, which are homologous to the HtrA protein of the model bacterium Escherichia coli. They are widely distributed in organisms including humans, prokaryotes and eukaryotes. Moreover, HtrA family proteins are important regulators of a variety of human physiological processes, which contains the maintenance of mitochondrial homeostasis, cellular signal transduction and apoptosis regulation. The HtrA family has been found to be associated with cancer and could be used as a target for future cancer treatments. The purpose of this article is to review the relationship between these HtrA and cancer and to summarize the latest researches on HtrA and cancer.
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http://dx.doi.org/10.1016/j.biopha.2021.111603DOI Listing
July 2021

Chiral Hypervalent Iodine Catalysis Enables an Unusual Regiodivergent Intermolecular Olefin Aminooxygenation.

JACS Au 2021 Jun 29;1(6):734-741. Epub 2021 Apr 29.

Department of Chemistry and Biochemistry, School of Green Chemistry and Engineering, The University of Toledo, 2801 West Bancroft Street, Toledo, Ohio 43606, United States.

A novel iodide-catalyzed intermolecular aminooxygenation strategy is described here. Amide is used as the O- and N- source to probe for regiocontrol strategies. Notably, simple additives can be selectively introduced to achieve regiodivergent oxyamination processes for electronically activated alkenes while being regio-complementary for unactivated alkenes. Our preliminary data demonstrates that this regiocontrol strategy based on nucleophile can also be applied in asymmetric processes using chiral hypervalent iodine catalysis.
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http://dx.doi.org/10.1021/jacsau.1c00103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243328PMC
June 2021

Prostaglandins for Postpartum Hemorrhage: Pharmacology, Application, and Current Opinion.

Pharmacology 2021 Jul 8:1-11. Epub 2021 Jul 8.

Women's Hospital, Medicine of School, Zhejiang University, Hangzhou, China.

Background: Postpartum hemorrhage (PPH) remains a common cause of maternal mortality worldwide. Medical intervention plays an important role in the prevention and treatment of PPH. Prostaglandins (PGs) are currently recommended as second-line uterotonics, which are applied in cases of persistent bleeding despite oxytocin treatment.

Summary: PG agents that are constantly used in clinical practice include carboprost, sulprostone, and misoprostol, representing the analogs of PGF2α, PGE2, and PGE1, respectively. Injectable PGs, when used to treat PPH, are effective in reducing blood loss but probably induce cardiovascular or respiratory side effects. Misoprostol is characterized by oral administration, low cost, stability in storage, broad availability, and minimal side effects. It remains a treatment option for uterine atony in low-resource settings, but its effectiveness as a uterotonic for independent application may be limited. Key Messages: The present review article discusses the physiological roles of various natural PGs, evaluates the existing evidence of PG analogs in the prevention and treatment of PPH, and finally provides a reference to assist obstetricians in selecting appropriate uterotonics.
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http://dx.doi.org/10.1159/000516631DOI Listing
July 2021

Synthesis and evaluation of acetylferulic paeonol ester and ferulic paeonol ester as potential antioxidants to inhibit fish oil oxidation.

Food Chem 2021 Jun 16;365:130384. Epub 2021 Jun 16.

Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

Acetylferulic paeonol ester (APE) and ferulic paeonol ester (FPE) were synthesized, and their structures were confirmed by NMR, mass spectra, IR and UV-vis data. The antioxidant properties of the synthesized compounds were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and [(2-azino-bis (3-ethylbenzthiazoline)-6 -sulfonic acid] (ABTS) assay as well as the production of oxidation products (peroxides, conjugated dienes, thiobarbituric acid-reactive substances, free fatty acids and total aldehydes) in an elevated temperature (60 °C) storage trial of fish oil extracted from anchovy. Furthermore, the changes in fatty acid composition were monitored by gas chromatography-mass spectrometry. The results showed that APE was more effective in restraining fish oil oxidation compared to FPE, ferulic acid, paeonol and the commercial antioxidant-butylated hydroxytoluene (BHT). This study demonstrated molecular combinations obtained by covalent bonding two antioxidant active molecules can result in novel compounds with enhanced antioxidant activities.
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http://dx.doi.org/10.1016/j.foodchem.2021.130384DOI Listing
June 2021

Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase.

ACS Cent Sci 2021 Jun 28;7(6):973-979. Epub 2021 Apr 28.

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072 Hubei, China.

Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a "base substitution", which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification.
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http://dx.doi.org/10.1021/acscentsci.0c01504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227591PMC
June 2021

Current Perspectives on B Lymphocytes in the Immunobiology of Hepatocellular Carcinoma.

Front Oncol 2021 21;11:647854. Epub 2021 Jun 21.

Department of Anesthesiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Immune cells infiltrating tumors are capable of significantly impacting carcinogenesis through cancer promotion and anticancer responses. There are many aspects of hepatocellular carcinoma (HCC) related T lymphocytes that are undergoing extensive studies, whereas the effect exerted by B lymphocytes remains a less researched area. In this study, the latest research on the effect of B lymphocytes as they infiltrate tumors in relation to HCC is presented. Their prognosis-related importance is analyzed, along with their function in the tumor microenvironment (TME), as well as the way that B cell biology can be employed to help create a B cell therapy strategy for HCC.
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http://dx.doi.org/10.3389/fonc.2021.647854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256159PMC
June 2021

Effects of transjugular intrahepatic portosystemic shunt treatment of patients with liver cirrhosis and portal hypertension: Case series.

Medicine (Baltimore) 2021 Jul;100(27):e26610

Department of Gastroenterology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, China.

Rationale: Transjugular intrahepatic portosystemic shunt (TIPS) is well established as an effective treatment tool for portal hypertension. However, the effects of TIPS in patients with liver cirrhosis and portal hypertension have not been adequately verified in clinical trials.

Patient Concerns: To evaluate the effects of TIPS in patients with liver cirrhosis and portal hypertension with or without portal vein thrombosis (PVT).

Interventions: A total of 55 patients with liver cirrhosis and portal hypertension received TIPS treatment from December 2014 to April 2018 were enrolled. Clinical data, including portal pressure, Child-Pugh score, and relevant complications were recorded.

Outcomes: TIPS was successfully performed in 54 patients. The overall technical success rate was 98.19% without serious technical complications. After TIPS treatment, portal pressure was significantly reduced from 38.13 ± 4.00 cmH2O to 24.14 ± 3.84 cmH2O (P < 0.05). In addition, symptoms including gastrointestinal bleeding and ascites were improved after TIPS treatment. During the 6 to 21-month follow up, hepatic encephalopathy in 15 patients (27.8%), shunt dysfunction in 5 patients (9.3%), rebleeding in 12 patients (22.2%) and deterioration of liver function in 2 patients (3.7%) were recorded. Moreover, there were no significant differences in the rates of rebleeding and hepatic encephalopathy between patients with PVT and the non-PVT group, whereas the occurrence rate of TIPS dysfunction was higher in the PVT group.

Lessons: TIPS treatment could alleviate the symptoms of liver cirrhosis and portal hypertension in individuals with or without PVT. However, complications during follow-up should be appropriately noted and addressed with corresponding treatments.
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http://dx.doi.org/10.1097/MD.0000000000026610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270572PMC
July 2021

Intraoperative Quantitative Measurements for Bradykinesia Evaluation during Deep Brain Stimulation Surgery Using Leap Motion Controller: A Pilot Study.

Parkinsons Dis 2021 15;2021:6639762. Epub 2021 Jun 15.

Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, China.

Deep brain stimulation (DBS) has shown a remarkably high effectiveness for Parkinson's disease (PD). In many PD patients during DBS surgery, the therapeutic effects of the stimulation test are estimated by assessing changes in bradykinesia as the stimulation voltage is increased. In this study, we evaluated the potential of the leap motion controller (LMC) to quantify the motor component of bradykinesia in PD during DBS surgery, as this could make the intraoperative assessment of bradykinesia more accurate. Seven participants with idiopathic PD receiving chronic bilateral subthalamic nucleus deep brain stimulation (DBS) therapy were recruited. The motor tasks of finger tapping (FT), hand opening and closing (OC), and hand pronation and supination (PS) were selected pre- and intraoperatively in accordance with the Movement Disorder Society revision of the Unified Parkinson's Disease Rating Scale. During the test, participants performed these tasks in sequence while being simultaneously monitored by the LMC and two professional clinicians. Key kinematic parameters differed between the preoperative and intraoperative conditions. We suggest that the average velocity ( ) and average amplitude ( ) of PS isolate the bradykinetic feature from that movement to provide a measure of the intraoperative state of the motor system. The LMC achieved promising results in evaluating PD patients' hand and finger bradykinesia during DBS surgery.
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http://dx.doi.org/10.1155/2021/6639762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221890PMC
June 2021

Superior All-Solid-State Batteries Enabled by a Gas-Phase-Synthesized Sulfide Electrolyte with Ultrahigh Moisture Stability and Ionic Conductivity.

Adv Mater 2021 Jul 3:e2100921. Epub 2021 Jul 3.

Beijing Advanced Innovation Center for Materials Genome Engineering, Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China.

Sulfide solid electrolytes (SEs) are recognized as one of the most promising candidates for all-solid-state batteries (ASSBs), due to their superior ionic conductivity and remarkable ductility. However, poor air stability, complex synthesis process, low yield, and high production cost obstruct the large-scale application of sulfide SEs. Herein, a one-step gas-phase synthesis method for sulfide SEs with oxide raw materials in ambient air, completely getting rid of the glovebox and thus making large-scale production possible, is reported. By adjusting substituted elements and concentrations, the ionic conductivity of Li Sn M S can reach 2.45 mS cm , which represents the highest value among all reported moist-air-stable and recoverable lithium-ion sulfide SEs reported. Furthermore, ASSBs with air/water-exposed and moderate-temperature-treated Li Sn As S even maintains superior performances with the highest reversible capacity (188.4 mAh g ) and the longest cycle life (210 cycles), which also breaks the record. Therefore, it may become one of the most critical breakthroughs during the development of sulfide ASSBs toward its practical application and commercialization.
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http://dx.doi.org/10.1002/adma.202100921DOI Listing
July 2021

Serum epidermal growth factor-like domain 7 serves as a novel diagnostic marker for early hepatocellular carcinoma.

BMC Cancer 2021 Jul 3;21(1):772. Epub 2021 Jul 3.

Department of Surgery, Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: Epidermal growth factor-like domain 7 (Egfl7), a recently identified secreted protein, was significantly increased in patients with HCC by our previous studies. However, its efficacy in the diagnosis of early HCC remains unknown. In this study, we therefore evaluate the efficacy of serum Egfl7 for early HCC diagnosis and compare it with alpha-fetoprotein (AFP).

Methods: Serum Egfl7 levels in testing cohort (1081 participants) and validation cohort (476 participants) were measured by a sandwich enzyme-linked immunoassay (ELISA). The cut-off value of Egfl7 was determined by Youden's index and the efficacies of Egfl7 and AFP in diagnosing early HCC were estimated by receiver operating characteristic (ROC).

Results: Serum Egfl7 was significantly elevated in patients with early HCC than all non-HCC controls in whatever Testing Cohort or Validation Cohort. In the Testing Cohort, ROC curves showed the optimum cut-off value of Egfl7 was 2610 ng/mL and Egfl7 showed a significantly higher sensitivity than AFP in discriminating early HCC from healthy individuals (77.4% vs. 65.3%, P = 0.0013) but the area under ROC (AUROC) and accuracy of Egfl7 and AFP were similar (0.860 vs. 0.868, P = 0.704; 80.2% vs. 83.8%, P = 0.184). In distinguishing patients with early HCC from patients with chronic liver disease (CLD), the AUROC, sensitivity, specificity and accuracy of Egfl7 were 0.800, 75.2, 71.7 and 73.5%, which were all significantly higher than AFP (0.675, 61.8, 62.0 and 61.9% in order). Egfl7 also showed a significant higher sensitivity and accuracy than AFP (76.6% vs. 64.0%, P = 0.0031; 79.9% vs. 66.1%, P < 0.0001) in differentiating early HCC patients from non-HCC individuals. Additionally, 70.8% of early HCC patients with negative AFP could be diagnosed by Egfl7 and the combined use of Egfl7 and AFP increased the sensitivity to 91.0%. These results were confirmed by a validation cohort.

Conclusion: Egfl7 is a valuable serum marker in the diagnosis of early HCC and could complement the efficacy of AFP, especially in distinguishing early HCC from CLD and identifying patients with AFP-negative early HCC.
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http://dx.doi.org/10.1186/s12885-021-08491-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255001PMC
July 2021

Efficacy and safety of surfactant administration via thin catheter in preterm infants with neonatal respiratory distress syndrome: A systematic review and meta-analysis.

Pediatr Pulmonol 2021 Jul 2. Epub 2021 Jul 2.

Department of Pediatrics, The Third Affifiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Objective: The efficacy and safety of surfactant administration via thin catheter in preterm infants with neonatal respiratory distress syndrome (NRDS) was investigated.

Methods: PubMed, Embase, Cochrane Library, and Web of Science databases were searched to identify randomized controlled trials (RCTs) that comparing thin catheter technique with intubation for surfactant delivery in preterm infants with NRDS.

Results: Thirteen RCTs (1931 infants) were included in the meta-analysis. The use of thin catheter technique decreased the incidences of bronchopulmonary dysplasia (BPD), pneumothorax, and hemodynamically significant patent ductus arteriosus (hsPDA) (risk ratio [RR]: 0.59, 95% confidence interval [CI]: 0.46-0.75, p < .0001; RR: 0.60, 95% CI: 0.39-0.93, p = .02 and RR: 0.88, 95% CI: 0.78-1.00, p = .04, respectively). In addition, infants in the intervention group required less mechanical ventilation within 72 h of life or during hospitalization (RR: 0.60, 95% CI: 0.48-0.75, p < .00001 and RR: 0.64, 95% CI: 0.49-0.82, p = .0005, respectively) compared with infants in the control group. However, the rate of surfactant reflux was higher in the intervention group than that in the control group (RR: 2.12, 95% CI: 1.37-3.29, p = .0008). There were no significant differences in mortality and other outcomes between the two groups.

Conclusion: The administration of surfactant via thin catheter could lower the requirement for mechanical ventilation, and decrease the incidence of BPD, pneumothorax, and hsPDA.
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http://dx.doi.org/10.1002/ppul.25545DOI Listing
July 2021

Prophylactic Norepinephrine Infusion Reduces Postoperative Complications and Hospitalization Time in Elderly Patients Undergoing Posterior Lumbar Spinal Fusion.

Biomed Res Int 2021 3;2021:2161036. Epub 2021 Jun 3.

Department of Anesthesiology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China.

This single-center prospective randomized controlled trial explores the effect of prophylactic norepinephrine infusion on the incidence of complications and hospitalization time in elderly patients (60-85 years old) undergoing posterior lumbar spinal fusion. In total, 129 elderly patients were randomized into two groups: a group that received norepinephrine during general anesthesia and a control group not receiving norepinephrine. The primary outcomes were in-hospital complications and 90-day postoperative complications and hospitalization time. The results show that in-hospital complications occurred in 24 of 60 patients (40%) in the control group versus 11 of 60 patients (18.3%) in the norepinephrine group (RR, 2.182; 95% CI, 1.177-4.045; = 0.015). Cardiac events occurred significantly more frequently in the control than in the norepinephrine group. Total number of patients experiencing complications within 90 days postoperatively was lower in the norepinephrine (11 of 60; 18.3%) than in the control group (26 of 60; 43.3%; RR, 2.364; 95% CI, 1.288-4.339; = 0.005). The median length of hospital stay was 17 days (11-27) in the control group and 15 days (10- 23) in the norepinephrine group ( = 0.01). The secondary outcomes were serum levels of syndecan-1, hyaluronic acid, heparan sulfate, and brain natriuretic peptide. Logistic regression analysis is used to describe the relationship between selected independent variables and in-hospital complications. Intraoperative total fluid, crystalloid, and colloid volumes were significantly higher in the control than in the norepinephrine group. The patients in the norepinephrine group had a higher MAP but a lower heart rate than those in the control group after the induction of anesthesia and intraoperatively. Syndecan-1, hyaluronic acid, and heparan sulfate serum levels showed a different course in the two groups. In conclusion, prophylactic norepinephrine infusion during posterior lumbar spinal fusion is preferable for elderly patients undergoing lumbar spinal fusion under general anesthesia. It can reduce postoperative complications and hospitalization time by reducing the injury to the vascular endothelium. This trial is registered with Clinical Trial Registration http://www.chictr.org.cn/showproj.aspx?proj=33660, identifier ChiCTR-1900021309.
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http://dx.doi.org/10.1155/2021/2161036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195642PMC
June 2021

Epigenetics: Roles and therapeutic implications of non-coding RNA modifications in human cancers.

Mol Ther Nucleic Acids 2021 Sep 1;25:67-82. Epub 2021 May 1.

Hepatobiliary/Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Transplantation, Chinese Academy of Medical Sciences, Nanjing, Jiangsu, China.

As next-generation sequencing (NGS) is leaping forward, more than 160 covalent RNA modification processes have been reported, and they are widely present in every organism and overall RNA type. Many modification processes of RNA introduce a new layer to the gene regulation process, resulting in novel RNA epigenetics. The commonest RNA modification includes pseudouridine (Ψ), -methylguanosine (m7G), 5-hydroxymethylcytosine (hm5C), 5-methylcytosine (m5C), -methyladenosine (m1A), -methyladenosine (m6A), and others. In this study, we focus on non-coding RNAs (ncRNAs) to summarize the epigenetic consequences of RNA modifications, and the pathogenesis of cancer, as diagnostic markers and therapeutic targets for cancer, as well as the mechanisms affecting the immune environment of cancer. In addition, we summarize the current status of epigenetic drugs for tumor therapy based on ncRNA modifications and the progress of bioinformatics methods in elucidating RNA modifications in recent years.
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http://dx.doi.org/10.1016/j.omtn.2021.04.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217334PMC
September 2021

Perspectives on Immunotherapy of Metastatic Colorectal Cancer.

Front Oncol 2021 9;11:659964. Epub 2021 Jun 9.

Hepatobiliary/Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Colorectal cancer, especially liver metastasis, is still a challenge worldwide. Traditional treatment such as surgery, chemotherapy and radiotherapy have been difficult to be further advanced. We need to develop new treatment methods to further improve the poor prognosis of these patients. The emergence of immunotherapy has brought light to mCRC patients, especially those with dMMR. Based on several large trials, some drugs (pembrolizumab, nivolumab) have been approved by US Food and Drug Administration to treat the patients diagnosed with dMMR tumors. However, immunotherapy has reached a bottleneck for other MSS tumors, with low response rate and poor PFS and OS. Therefore, more clinical trials are underway toward mCRC patients, especially those with MSS. This review is intended to summarize the existing clinical trials to illustrate the development of immunotherapy in mCRC patients, and to provide a new thinking for the direction and experimental design of immunotherapy in the future.
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http://dx.doi.org/10.3389/fonc.2021.659964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219967PMC
June 2021
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