Publications by authors named "Fan Liu"

784 Publications

RNAi silencing of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene inhibits vitellogenesis in Chinese mitten crab Eriocheir sinensis.

Comp Biochem Physiol A Mol Integr Physiol 2021 Sep 15:111078. Epub 2021 Sep 15.

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. Electronic address:

The sesquiterpenoid methyl farnesoate (MF), a de-epoxide form of insect juvenile hormone III (JH III), plays an essential role in regulating many crucial physiological processes in crustaceans including vitellogenesis and reproduction. 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is an important rate-limiting enzyme in the mevalonate pathway, which is critical for the synthesis of JH III and MF. In the present study, a full-length cDNA encoding HMGR (EsHMGR) in Eriocheir sinensis was isolated and characterised. Sequence analysis of EsHMGR revealed that it belongs to Class I HMGR family proteins with HMG-CoA-binding and NADPH-binding domains, both important for HMGR activity. In addition to its ubiquitous tissue expression, expression of EsHMGR was highly specific to the ovary, the main site of Vg synthesis. During ovarian development, EsHMGR expression in ovary displayed a stage-specific pattern, and was correlated with expression of vitellogenin (EsVg) in hepatopancreas, which suggests that EsHMGR possibly involved in vitellogenesis. To further investigate the functional role of EsHMGR in vitellogenin biosynthesis in E. sinensis, RNA interference-mediated gene silencing was carried out both in vitro and in vivo. Quantitative PCR results showed that injection of EsHMGR double-stranded RNA (dsRNA) led to a significant decrease in EsVg expression levels in ovary and hepatopancreas both in vitro and in vivo. Taken together, the results suggest that EsHMGR is involved in vitellogenin biosynthesis in female E. sinensis, which may provide a new resource for HMGR enzymes participating in reproduction in crustaceans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpa.2021.111078DOI Listing
September 2021

High-performance anti-haze window screen based on multiscale structured polyvinylidene fluoride nanofibers.

J Colloid Interface Sci 2021 Sep 9;607(Pt 1):711-719. Epub 2021 Sep 9.

Textile and Garment Industry of Research Institute, Zhongyuan University of Technology, Zhengzhou 450007, People's Republic of China; International Joint Laboratory of New Textile Materials and Textiles of Henan Province, Zhengzhou 450007, People's Republic of China. Electronic address:

Indoor air quality (IAQ) has assumed new significance given the extensive amount of time spent indoor due to the coronavirus pandemic and particulate matter (PM) pollution. Accordingly, the development of window air filters to effectively intercept PM from outdoor air under natural ventilation conditions is an important research topic. However, most existing filters inevitably suffer from the compromise among filtration capability, transparency, and air permeability. In this study, we fabricate a high-performance transparent air filter to improve IAQ via natural ventilation. polyvinylidene fluoride (PVDF) superfine nanofibers of size 20-35 nm are prepared using extremely dilute solution electrospinning; a multi-scale nanofiber structure is then designed. By adjusting the ratio of PVDF superfine nanofibers (SNs) to PVDF coarse fibers (CNs), we balance the structure-performance relationship. Benefiting from the multiscale structural features that include a small pore size (0.72 μm) and high porosity (92.22%), the resulting filters exhibit excellent performance including high interception efficiency (99.92%) for PM, low air resistance (69 Pa), high transparency (∼80%) and stable filtration after 100 h of UV irradiation. This work describes a new strategy for the fabrication of nanofibers with true-nanoscale diameters and the design of high-performance air filters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.09.040DOI Listing
September 2021

Identification of novel loci influencing refractive error in East Asian populations using an extreme phenotype design.

J Genet Genomics 2021 Sep 11. Epub 2021 Sep 11.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510000, China. Electronic address:

The global "myopia boom" has raised significant international concerns. Despite a higher myopia prevalence in Asia, previous large-scale genome-wide association studies (GWASs) were mostly based on European descendants. Here, we report a GWAS of spherical equivalent (SE) in 1852 Chinese Han individuals with extreme SE from Guangzhou (631 < -6D and 574 > 0D) and Wenzhou (593 < -6D and 54 > -1.75 D), followed by a replication study in two independent cohorts with totaling 3538 East Asian individuals. The discovery GWAS and meta-analysis identify three novel loci which show genome-wide significant associations with SE, including 1q25.2 FAM163A, 10p11.22 NRP1/PRAD3, and 10p11.21 ANKRD30A/MTRNR2L7, together explaining 3.34% of SE variance. 10p11.21 was successfully replicated. The allele frequencies of all three loci show significant differences between major continental groups (P < 0.001). The SE reducing (more myopic) allele of rs10913877 (1q25.2 FAM163A) demonstrates the highest frequency in East Asians and much lower frequencies in Europeans and Africans (EAS = 0.60, EUR = 0.20, AFR = 0.18). The gene-based analysis additionally identifies three novel genes associated with SE, including EI24, LHX5 and ARPP19. These results provide new insights into myopia pathogenesis, and indicate the role of genetic heterogeneity in myopia epidemiology among different ethnicities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2021.08.011DOI Listing
September 2021

A Novel Dual-Stage Phase Separation Process for CO Absorption into a Biphasic Solvent with Low Energy Penalty.

Environ Sci Technol 2021 Sep 13. Epub 2021 Sep 13.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.

An amine-based biphasic solvent is promising to cut down the energy penalty of CO capture. However, the high viscosity of the CO-enriched solvent retards its industrial application. This work proposed a novel dual-stage phase separation process using a triethylenetetramine and 2-(diethylamino)ethanol blend as a biphasic solvent, which separates a certain proportion of CO-enriched phase during CO absorption to reduce its viscosity. Experimental results showed that the proposed dual-stage phase separation process improved the phase separation behavior and effectively enhanced the absorption rate by 49% at 50 °C, when 50 vol % CO-enriched phase was separated at 0.3 mol mol. Kinetic analysis showed that the absorption rate was mainly controlled by liquid-side mass transfer. The regeneration heat of the dual-stage phase separation process cut down the energy penalty by 33% compared with the monoethanolamine-based process. Compared with the conventional biphasic solvent-based process, the heat duty was further declined by 8%. The H nuclear magnetic resonance analysis showed that the dual-stage phase separation process could effectively control the generation of absorption products and intensify the interphase migration of tertiary amines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.1c01622DOI Listing
September 2021

Smoothened is a therapeutic target for reducing glutamate toxicity in ischemic stroke.

Sci Transl Med 2021 Sep 8;13(610):eaba3444. Epub 2021 Sep 8.

The Brain Science Center, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.

[Figure: see text].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/scitranslmed.aba3444DOI Listing
September 2021

Retraction Note: Predicting adult height from DNA variants in a European-Asian admixed population.

Int J Legal Med 2021 Sep 7. Epub 2021 Sep 7.

National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics of Ministry of Public Security, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00414-021-02692-yDOI Listing
September 2021

Biodegradable and high-performance multiscale structured nanofiber membrane as mask filter media via poly(lactic acid) electrospinning.

J Colloid Interface Sci 2021 Aug 17;606(Pt 2):961-970. Epub 2021 Aug 17.

Textile and Garment Industry of Research Institute, Zhongyuan University of Technology, Zhengzhou 450007, People's Republic of China; International Joint Laboratory of New Textile Materials and Textiles of Henan Province, Zhengzhou 450007, People's Republic of China.

The usage of single-use face masks (SFMs) has increased since the outbreak of the coronavirus pandemic. However, non-degradability and mismanagement of SFMs have raised serious environmental concerns. Moreover, both melt-blown and nanofiber-based mask filters inevitably suffer from poor filtration performance, like a continuous decrease in the removal efficiency for particulate matter (PM) and weak breathability. Herein, we report a new method to create biodegradable and reusable fibrous mask filters. The filter consists of a true nanoscale bio-based poly(lactic acid) (PLA) fiber (an average size of 37 ± 4 nm) that is fabricated via electrospinning of an extremely dilute solution. Furthermore, we designed a multiscale structure with integrated features, such as low basis weight (0.91 g m), small pore size (0.73 μm), and high porosity (91.72%), formed by electrospinning deposition of true nanoscale fibers on large pore of 3D scaffold nanofiber membranes. The resultant mask filter exhibited a high filtration efficiency (PM-99.996%) and low pressure drop (104 Pa) superior to the commercial N95 filter. Importantly, this filter has a durable filtering efficiency for PM and natural biodegradability based on PLA. Therefore, this study offers an innovative strategy for the preparation of PLA nanofibers and provides a new design for high-performance nanofiber filters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.08.079DOI Listing
August 2021

Long Non-coding RNA N1LR Protects Against Myocardial Ischemic/Reperfusion Injury Through Regulating the TGF-β Signaling Pathway.

Front Cardiovasc Med 2021 13;8:654969. Epub 2021 Aug 13.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.

Long non-coding RNAs (lncRNAs) have been shown to play critical roles in various cell biological processes. However, the mechanism of lncRNAs in acute myocardial infarction (AMI) is not fully understood. Previous studies showed that lncRNA N1LR was down-regulated in ischemic cerebral stroke and its up-regulation was protective. The current study was designed to assess the protective effect of N1LR and further to explore potential mechanisms of N1LR in ischemic/reperfusion (I/R) injury after AMI. Male C57BL/6J mice and H9c2 cardiomyocytes were selected to construct and pathological models. In H9c2 cell line, N1LR expression was markedly decreased after HO and CoCl treatments and N1LR overexpression alleviated apoptosis, inflammation reaction, and LDH release in cardiomyocytes treated with HO and CoCl. Mouse study showed that overexpression of N1LR enhanced cardiac function and suppressed inflammatory response and fibrosis. Mechanistically, we found that the expression of transforming growth factor (TGF)-β1 and smads were significantly decreased in the N1LR overexpression group exposed to HO. In a summary, our study indicated that N1LR can act as a protective factor against cardiac ischemic-reperfusion injury through regulating the TGF-β/Smads signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.654969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414635PMC
August 2021

Detection of an anti-angina therapeutic module in the effective population treated by a multi-target drug Danhong injection: a randomized trial.

Signal Transduct Target Ther 2021 Sep 1;6(1):329. Epub 2021 Sep 1.

Department of Cardiology, Affiliated Hospital of Shanxi University of Chinese Medicine, Xianyang, Shanxi, China.

It's a challenge for detecting the therapeutic targets of a polypharmacological drug from variations in the responsed networks in the differentiated populations with complex diseases, as stable coronary heart disease. Here, in an adaptive, 31-center, randomized, double-blind trial involving 920 patients with moderate symptomatic stable angina treated by 14-day Danhong injection(DHI), a kind of polypharmacological drug with high quality control, or placebo (0.9% saline), with 76-day following-up, we firstly confirmed that DHI could increase the proportion of patients with clinically significant changes on angina-frequency assessed by Seattle Angina Questionnaire (ΔSAQ-AF ≥ 20) (12.78% at Day 30, 95% confidence interval [CI] 5.86-19.71%, P = 0.0003, 13.82% at Day 60, 95% CI 6.82-20.82%, P = 0.0001 and 8.95% at Day 90, 95% CI 2.06-15.85%, P = 0.01). We also found that there were no significant differences in new-onset major vascular events (P = 0.8502) and serious adverse events (P = 0.9105) between DHI and placebo. After performing the RNA sequencing in 62 selected patients, we developed a systemic modular approach to identify differentially expressed modules (DEMs) of DHI with the Z value less than 0 compared with the control group, calculated by weighted gene co-expression network analysis (WGCNA), and sketched out the basic framework on a modular map with 25 functional modules targeted by DHI. Furthermore, the effective therapeutic module (ETM), defined as the highest correlation value with the phenotype alteration (ΔSAQ-AF, the change in SAQ-AF at Day 30 from baseline) calculated by WGCNA, was identified in the population with the best effect (ΔSAQ-AF ≥ 40), which is related to anticoagulation and regulation of cholesterol metabolism. We assessed the modular flexibility of this ETM using the global topological D value based on Euclidean distance, which is correlated with phenotype alteration (r: 0.8204, P = 0.019) by linear regression. Our study identified the anti-angina therapeutic module in the effective population treated by the multi-target drug. Modular methods facilitate the discovery of network pharmacological mechanisms and the advancement of precision medicine. (ClinicalTrials.gov identifier: NCT01681316).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-021-00741-xDOI Listing
September 2021

Retraction Note: EDAR, LYPLAL1, PRDM16, PAX3, DKK1, TNFSF12, CACNA2D3, and SUPT3H gene variants influence facial morphology in a Eurasian population.

Hum Genet 2021 Aug 30. Epub 2021 Aug 30.

Key Laboratory of Forensic Genetics, National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00439-021-02352-6DOI Listing
August 2021

Perceptions of orthodontists, laypersons, and patients regarding buccal corridors and facial types.

Am J Orthod Dentofacial Orthop 2021 Aug 24. Epub 2021 Aug 24.

Department of Orthodontics and Prosthodontics, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Introduction: The space on each side of the mouth, between the commissure of the mouth and the buccal surface of the last visible tooth, is called the buccal corridor. The size of the buccal corridor can affect perceptions of facial esthetics. We analyzed the perceptions of orthodontists, laypersons, and orthodontic patients regarding the size of buccal corridors in people with brachyfacial, mesofacial, and dolichofacial types.

Methods: Photographs of 1 male and 1 female model were modified digitally into brachyfacial, mesofacial, and dolichofacial types. Each facial type was further modified to create 5 sizes of buccal corridors. Orthodontists, adult orthodontic patients, and laypersons were invited to rate the photographs.

Results: Orthodontists rated lower than the other groups of raters when they evaluated the female model with the buccal corridors of 20% (P <0.05). Laypersons could not distinguish the changes of the buccal corridors when evaluating the male model with brachyfacial and mesofacial (P >0.05). Orthodontic patients rated significantly higher than the orthodontists and the laypersons when evaluating the dolichofacial type of both models (P <0.05).

Conclusions: Orthodontists are more sensitive to the buccal corridors than are orthodontic patients and laypersons. It is only necessary to consider eliminating the patient's buccal corridors when the buccal corridor area ratio is over 15%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajodo.2020.06.051DOI Listing
August 2021

Acupuncture treatment on attention deficit hyperactivity disorder: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Aug;100(34):e27033

Yunnan University of Chinese Medicine, 88 Baita Road, Panlong District, Kunming city, Yunnan Province, China.

Background: Attention Deficit Hyperactivity Disorder (ADHD) is the most common behavioral disorder in childhood. Acupuncture treatment of ADHD has formed a relatively systematic theoretical and clinical treatment system which achieved satisfactory results. However, there has been no systematic evaluation of its effectiveness and safety. The purpose of this study was to evaluate the efficacy and safety of acupuncture in the treatment of ADHD.

Methods: A systematic search of literature will be conducted in PubMed, Cochrane Library, the Web of Science, Excerpt Medica Database, Chinese Biomedical Literature Database, VIP, Wanfang database, China National Knowledge Infrastructure database for articles published up to September, 2019. The searching terms include "attention deficit", "hyperactivity", "mild brain dysfunction", "acupuncture", "electroacupuncture". The search is limited to studies published in Chinese and English. Two reviewers will extract and evaluate the information independently. Cochrane Collaboration tool and Jadad scale will be used to evaluate the quality of the studies. Review Manager Version 5.3 (Cochrane Collaboration's software) will be used to carry out the meta-analysis.

Results: High-quality synthesis and/or descriptive analysis of current evidence will be provided from effective rate, total score of traditional Chinese medicines syndromes, conners child hyperactivity-diagnosis rating scale, conners index of hyperactivity, the recurrence rate, and adverse events.

Conclusion: This study will provide the evidence of whether acupuncture is an effective and safe intervention to ADHD.

Inplasy Registration Number: INPLASY202140022.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000027033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389897PMC
August 2021

Clinical impact of craniopharyngioma classification based on location origin: a multicenter retrospective study.

Ann Transl Med 2021 Jul;9(14):1164

Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: An assessment of the clinical impact for craniopharyngiomas (CPs) classification based on origin location has not been reported. The aim of this study was to determine the clinical impact of the site of tumor origin in primary CPs.

Methods: Patients from six national institutions who had undergone resection for primary CP were enrolled. Based on the point of origin and surrounding membranous structures, the location of the tumor origin was labelled as Q, S, or T, where Type Q CPs originated below the diaphragmatic area; Type S CPs originated from Rathke's pouch precursor cells; and Type T CPs originated from the Rathke's pouch precursor cells located above the pars tuberalis. Clinical characteristics, surgical approach, and outcome were evaluated according to the location of the tumor origin.

Results: Among the 529 patients with primary CP, symptoms, age, histopathology type, tumor size, the incidence of hydrocephalus, survival rates, and recurrence-free survival rates were significantly different among tumors originating in different locations. Patients with type T CPs had higher symptom rates of intracranial hypertension and hypothalamic dysfunction, while those with type Q CPs had higher rates of hormone deficits during pre-and post-operative management. Type S CPs were correlated with better outcomes and lower recurrence rates. The location of origin and primary therapy with survival and recurrence in CP were independent factors for survival and recurrence in multivariate analysis.

Conclusions: The identification of the different location of origin of CPs is of great significance in understanding the relationship between tumors and peripheral tissues. The origin of tumors effects the choice of surgical approach and prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-2924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350632PMC
July 2021

Extended short-range airborne transmission of respiratory infections.

J Hazard Mater 2021 Aug 6;422:126837. Epub 2021 Aug 6.

Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China. Electronic address:

Debate and scientific inquiries regarding airborne transmission of respiratory infections such as COVID-19 and influenza continue. Health authorities including the WHO and the US CDC have recognized the airborne transmission of COVID-19 in specific settings, although the ventilation requirements remain to be determined. In this work we consider the long-range airborne transmission as an extended short-range airborne route, which reconciles the link between short- and long-range airborne routes. The effective short-range distance is defined as the distance in short range at which long-range route has the same volumetric exposure value as that due to short-range route. Our data show that a decrease in ventilation rate or room volume per person, or an increase in the ratio of the number of infected to susceptible people reduces the effective short-range distance. In a normal breathing scenario with one out of five people infected and a room volume of 12 m per person to ensure an effective short-range distance of 1.5 m, a ventilation rate of 10 L/s per person is needed for a duration of 2 h. Our results suggest that effective environmental prevention strategies for respiratory infections require appropriate increases in the ventilation rate while maintaining a sufficiently low occupancy. PRACTICAL IMPLICATIONS: Demonstration of the long-range airborne route as an extended short-range airborne route suggests the significant role played by building ventilation in respiratory infection exposure. The reconciliation of short- and long-range airborne transmission suggests that the commonly observed dominance of close-contact transmission is a probable evidence of short-range airborne transmission, following a separate earlier study that revealed the relative insignificance of large droplet transmission in comparison with the short-range airborne-route. Existing ventilation standards do not account for respiratory infection control, and this study presents a possible approach to account for infection under new ventilation standards.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342890PMC
August 2021

Identification and characterization of early Fusarium wilt responsive mRNAs and long non-coding RNAs in banana root using high-throughput sequencing.

Sci Rep 2021 Aug 11;11(1):16363. Epub 2021 Aug 11.

Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Fusarium wilt disease, caused by Fusarium oxysporum f.sp. cubense (Foc), has been recognized as the most devastating disease to banana. The regulatory role of long non-coding RNAs (lncRNAs) in plant defense has been verified in many plant species. However, the understanding of their role during early FocTR4 (Foc tropical race 4) infection stage is very limited. In this study, lncRNA sequencing was used to reveal banana root transcriptome profile changes during early FocTR4 infection stages. Quantitative real time PCR (qRT-PCR) was performed to confirm the expression of eight differentially expressed (DE) lncRNAs (DELs) and their predicted target genes (DETs), and three DE genes (DEGs). Totally, 12,109 lncRNAs, 36,519 mRNAs and 2642 novel genes were obtained, of which 1398 (including 78 DELs, 1220 DE known genes and 100 DE novel genes) were identified as FocTR4 responsive DE transcripts. Gene function analysis revealed that most DEGs were involved in biosynthesis of secondary metabolites, plant-pathogen interaction, plant hormone signal transduction, phenylalanine metabolism, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and so on. Coincidently, many DETs have been identified as DEGs in previous transcriptome studies. Moreover, many DETs were found to be involved in ribosome, oxidative phosphorylation, lipoic acid metabolism, ubiquitin mediated proteolysis, N-glycan biosynthesis, protein processing in endoplasmic reticulum and DNA damage response pathways. QRT-PCR result showed the expression patterns of the selected transcripts were mostly consistent with our lncRNA sequencing data. Our present study showed the regulatory role of lncRNAs on known biotic and abiotic stress responsive genes and some new-found FocTR4 responsive genes, which can provide new insights into FocTR4-induced changes in the banana root transcriptome during the early pathogen infection stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-95832-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358008PMC
August 2021

Linkage of nanosecond protein motion with enzymatic methyl transfer by nicotinamide N-methyltransferase.

Turk J Biol 2021 23;45(3):333-341. Epub 2021 Jun 23.

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin China.

Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N-methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking. Here, wild-type nicotinamide N-methyltransferase together with the mutation at position 20 with Y20F, Y20G, and free tryptophan were carried out to explore the connection between protein dynamics and catalysis using time-resolved fluorescence lifetimes. The results show that wild-type nicotinamide N-methyltransferase prefers to adapt a less flexible protein conformation to achieve enzyme catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3906/biy-2101-54DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313939PMC
June 2021

Cadmium Isotope Fractionation during Adsorption and Substitution with Iron (Oxyhydr)oxides.

Environ Sci Technol 2021 Sep 9;55(17):11601-11611. Epub 2021 Aug 9.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtse River), Ministry of Agriculture and Rural Affairs, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.

Cadmium (Cd) isotopes have great potential for understanding Cd geochemical cycling in soil and aquatic systems. Iron (oxyhydr)oxides can sequester Cd via adsorption and isomorphous substitution, but how these interactions affect Cd isotope fractionation remains unknown. Here, we show that adsorption preferentially enriches lighter Cd isotopes on iron (oxyhydr)oxide surfaces through equilibrium fractionation, with a similar fractionation magnitude (ΔCd) for goethite (Goe) (-0.51 ± 0.04‰), hematite (Hem) (-0.54 ± 0.10‰), and ferrihydrite (Fh) (-0.55 ± 0.03‰). Neither the initial Cd concentration or ionic strength nor the pH influence the fractionation magnitude. The enrichment of the light isotope is attributed to the adsorption of highly distorted [CdO] on solids, as indicated by Cd K-edge extended X-ray absorption fine-structure analysis. In contrast, Cd incorporation into Goe by substitution for lattice Fe at a Cd/Fe molar ratio of 0.05 preferentially sequesters heavy Cd isotopes, with a ΔCd of 0.22 ± 0.01‰. The fractionation probably occurs during the transformation of Fh into Goe via dissolution and reprecipitation. These results improve the understanding of the Cd isotope fractionation behavior being affected by iron (oxyhydr)oxides in Earth's critical zone and demonstrate that interactions with minerals can obscure anthropogenic and natural Cd isotope characteristics, which should be carefully considered when applying Cd isotopes as environmental tracers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c06927DOI Listing
September 2021

Literary runaway: Increasingly more references cited per academic research article from 1980 to 2019.

PLoS One 2021 6;16(8):e0255849. Epub 2021 Aug 6.

Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.

References are employed in most academic research papers to give credits and to reflect scholarliness. With the upsurge in academic publications in recent decades, we are curious to know how the number of references cited per research article has changed across different disciplines over that time. The results of our study showed significant linear growth in reference density in eight disciplinary categories between 1980 and 2019 indexed in Web of Science. It appears that reference saturation is not yet in sight. Overall, the general increase in the number of publications and the advanced accessibility of the Internet and digitized documents may have promoted the growth in references in certain fields. However, the seemingly runaway tendency should be well appreciated and objectively assessed. We suggest that authors focus on their research itself rather than on political considerations during the process of writing, especially the selection of important references to cite.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255849PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345841PMC
August 2021

Compensatory feeding during early gestation for sows with a high weight loss after a summer lactation increased piglet birth weight but reduced litter size.

J Anim Sci 2021 Sep;99(9)

Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.

Sows mated in summer produce a greater proportion of born-light piglets (<1.1 kg) which contributes to increased carcass fatness in the progeny population. The reasons for the low birth weight of these piglets remain unclear, and there have been few successful mitigation strategies identified. We hypothesized that: 1) the low birth weight of progeny born to sows mated in summer may be associated with weight loss during the previous summer lactation; and 2) increasing early gestation feed allowance for the sows with high lactational weight loss in summer can help weight recovery and improve progeny birth weight. Sows were classified as having either low (av. 1%) or high (av. 7%) lactational weight loss in their summer lactation. All the sows with low lactational weight loss (LLStd) and half of the sows with high lactational weight loss received a standard gestation feeding regime (HLStd) (2.6 kg/d; day 0-30 gestation), whereas the rest of the sows with high lactational weight loss received a compensatory feed allowance (HLComp) (3.5 kg/d; day 0-30 gestation). A comparison of LLStd (n = 75) versus HLStd sows (n = 78) showed that this magnitude of weight loss over summer lactation did not affect the average piglet or litter birth weight, but such results may be influenced by the higher litter size (P = 0.030) observed in LLStd sows. A comparison of HLStd versus HLComp (n = 81) sows showed that the compensatory feeding increased (P = 0.021) weight gain of gestating sows by 6 kg, increased (P = 0.009) average piglet birth weight by 0.12 kg, tended to reduce (P = 0.054) the percentage of born-light piglets from 23.5% to 17.1% but reduced the litter size by 1.4 (P = 0.014). A subgroup of progeny stratified as born-light (0.8-1.1 kg) or -normal (1.3-1.7 kg) from each sow treatment were monitored for growth performance from weaning until 100 kg weight. The growth performance and carcass backfat of progeny were not affected by sow treatments. Born-light progeny had lower feed intake, lower growth rate, higher G:F, and higher carcass backfat than born-normal progeny (all P < 0.05). In summary, compensatory feeding from day 0 to 30 gestation in the sows with high weight loss during summer lactation reduced the percentage of born-light progeny at the cost of a lower litter size, which should improve growth rate and carcass leanness in the progeny population born to sows with high lactational weight loss.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jas/skab228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418633PMC
September 2021

A little consideration for "Rare presentations of COVID-19: PRES-like leukoencephalopathy and carotid thrombosis".

Clin Imaging 2021 Jul 24;80:148-149. Epub 2021 Jul 24.

Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410008, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinimag.2021.06.038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302474PMC
July 2021

Modern honey bees disrupt the pollination of an ancient gymnosperm, Gnetum luofuense.

Ecology 2021 Jul 27:e03497. Epub 2021 Jul 27.

State Key Laboratory of Hybrid Rice, Department of Ecology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ecy.3497DOI Listing
July 2021

Manipulated Crystallization and Passivated Defects for Efficient Perovskite Solar Cells via Addition of Ammonium Iodide.

ACS Appl Mater Interfaces 2021 Jul 13;13(29):34053-34063. Epub 2021 Jul 13.

Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin, Engineering Research Center of Thin Film Optoelectronics Technology, Ministry of Education, Institute of Photoelectronic Thin Film Devices and Technology of Nankai University, Solar Energy Research Center of Nankai University, #38 Tongyan Road, Jinnan District, Tianjin 300350, China.

Organic-inorganic metal halide perovskite materials have been widely studied as the light absorber for efficient photovoltaics. However, perovskite layers with defective nature are typically prepared with an uncontrollable crystallization process, intrinsically limiting further advance in device performance, and thus require delicate manipulation of crystallization processes and defect density. Here, we demonstrate an ammonium-assisted crystallization of perovskite absorbers during a two-step deposition to fabricate efficient solar cells. Addition of ammonium iodide (NHI) is devised to manipulate the nucleation and crystal growth of perovskite, wherein the formation and transition of intermediate [NH]•[PbI] enables high-quality perovskite layers with an enlarged grain and reduced defect density. As a result, the perovskite solar cells (PSCs) achieve an average efficiency of 21.36% with a champion efficiency of 22.15% and improved environmental stability over 30 days in ambient conditions with varied relative humidity. These results with addition of NHI provide an available and ingenious way to construct high-quality perovskite layers for efficient solar cells and will advance the commercial application of perovskite-based photovoltaics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c05903DOI Listing
July 2021

The Welwitschia genome reveals a unique biology underpinning extreme longevity in deserts.

Nat Commun 2021 07 12;12(1):4247. Epub 2021 Jul 12.

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

The gymnosperm Welwitschia mirabilis belongs to the ancient, enigmatic gnetophyte lineage. It is a unique desert plant with extreme longevity and two ever-elongating leaves. We present a chromosome-level assembly of its genome (6.8 Gb/1 C) together with methylome and transcriptome data to explore its astonishing biology. We also present a refined, high-quality assembly of Gnetum montanum to enhance our understanding of gnetophyte genome evolution. The Welwitschia genome has been shaped by a lineage-specific ancient, whole genome duplication (~86 million years ago) and more recently (1-2 million years) by bursts of retrotransposon activity. High levels of cytosine methylation (particularly at CHH motifs) are associated with retrotransposons, whilst long-term deamination has resulted in an exceptionally GC-poor genome. Changes in copy number and/or expression of gene families and transcription factors (e.g. R2R3MYB, SAUR) controlling cell growth, differentiation and metabolism underpin the plant's longevity and tolerance to temperature, nutrient and water stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-24528-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275611PMC
July 2021

Periostin deficiency reduces diethylnitrosamine-induced liver cancer in mice by decreasing hepatic stellate cell activation and cancer cell proliferation.

J Pathol 2021 Oct 5;255(2):212-223. Epub 2021 Aug 5.

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, PR China.

Periostin is a critical extracellular regulator in the pathogenesis of liver disorders such as hepatosteatosis, non-alcoholic steatohepatitis, inflammation, and fibrosis. Periostin is also involved in the progression of hepatocellular carcinoma (HCC). However, the molecular mechanisms of periostin in hepatic stellate cell (HSC) activation and tumor cell proliferation in the pathogenesis of HCC remain largely unknown. We demonstrate that periostin is markedly upregulated in diethylnitrosamine (DEN)-induced mouse HCC tissues and that periostin knockout impairs DEN-induced HCC development. Periostin is predominantly derived from activated HSCs and periostin deficiency in HSCs impairs HSC activation and inhibits HSC-promoted HCC cell proliferation in vitro and tumor growth in vivo. Mechanistically, periostin promotes HSC activation through the integrin-FAK-STAT3-periostin pathway and augments HCC cell proliferation by activating ERK. There are positive correlations between periostin and HSC activation and cell proliferation in HCC clinical samples. Collectively, our findings demonstrate that HSC-derived periostin promotes HCC development by enhancing HSC activation through an autocrine periostin-integrin-FAK-STAT3-periostin circuit and by augmenting HCC cell proliferation via the ERK pathway in a paracrine manner. Thus, periostin is a multifaceted extracellular regulator in the development of HCC. © 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/path.5756DOI Listing
October 2021

Isolation and characterization of a strain with high microbial attachment in aerobic granular sludge.

J Environ Sci (China) 2021 Aug 5;106:194-203. Epub 2021 Feb 5.

School of Environment, Beijing Normal University, Beijing 100875, China; R & D Centre of Aerobic Granule Technology, Beijing 100875, China. Electronic address:

Aerobic granule is a special microbial aggregate associated with biofilm structure. The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties. In this experiment, a strain with high microbial attachment was isolated from aerobic granular sludge, and the detailed characteristics were examined. Its high attachment ability could reach 2.34 (OD), while other low attachment values were only around 0.06-0.32, which indicated a big variation among the different bacteria. The strain exhibited a very special morphology with many fibric fingers under SEM observation. A distinctive behaviour was to form a spherical particle by themselves, which would be very beneficial for the formation and development of granular sludge. The EPS measurement showed that its PN content was higher than low attachment bacteria, and 3D-EEM confirmed that there were some different components. Based on the 16S rRNA analysis, it was identified to mostly belong to Stenotrophomonas. Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule. Conclusively, it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2021.01.019DOI Listing
August 2021

SnRK1.1-mediated resistance of Arabidopsis thaliana to clubroot disease is inhibited by the novel Plasmodiophora brassicae effector PBZF1.

Mol Plant Pathol 2021 Sep 24;22(9):1057-1069. Epub 2021 Jun 24.

Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetics Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, China.

Plants have evolved a series of strategies to combat pathogen infection. Plant SnRK1 is probably involved in shifting carbon and energy use from growth-associated processes to survival and defence upon pathogen attack, enhancing the resistance to many plant pathogens. The present study demonstrated that SnRK1.1 enhanced the resistance of Arabidopsis thaliana to clubroot disease caused by the plant-pathogenic protozoan Plasmodiophora brassicae. Through a yeast two-hybrid assay, glutathione S-transferase pull-down assay, and bimolecular fluorescence complementation assay, a P. brassicae RxLR effector, PBZF1, was shown to interact with SnRK1.1. Further expression level analysis of SnRK1.1-regulated genes showed that PBZF1 inhibited the biological function of SnRK1.1 as indicated by the disequilibration of the expression level of SnRK1.1-regulated genes in heterogeneous PBZF1-expressing A. thaliana. Moreover, heterogeneous expression of PBZF1 in A. thaliana promoted plant susceptibility to clubroot disease. In addition, PBZF1 was found to be P. brassicae-specific and conserved. This gene was significantly highly expressed in resting spores. Taken together, our results provide new insights into how the plant-pathogenic protist P. brassicae employs an effector to overcome plant resistance, and they offer new insights into the genetic improvement of plant resistance against clubroot disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/mpp.13095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358996PMC
September 2021

Two Novel Mutations ( and ) Causing Coagulation Factor XII Deficiency in a Patient with Acute Inferior Myocardial Infarction.

Ann Clin Lab Sci 2021 May;51(3):426-429

Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

Objective: To identify the gene mutation of the coagulation factor XII (FXII) in a patient with FXII deficiency and acute inferior myocardial infarction.

Methods: The proband was a 51-year-old Chinese man who was diagnosed with acute inferior myocardial infarction and had a history of FXII deficiency. The patient presented with a prolonged activated partial thromboplastin time (160 s) and decreased FXII activity (2.3%) and FXII antigen (1%). DNA sequence analysis of the FXII gene was performed by next generation sequencing. The mutant FXII cDNAs were constructed in an expression plasmid vector and transfected into 293T cells. The expression of FXII antigen was detected by western blot.

Results: Sequencing of the FXII gene revealed two novel heterozygous mutations, one at exon 8 (G774A; p: W258X) and the other at exon 14 (A1685G; p: D562G). Western blot showed that the FXII antigens were detected only in the supernatant and whole cell lysate of the wild-type and A1685G mutant type, but not in G774A or G774A plus the A1685G mutant type. In addition, the results showed that secretion but not synthesis of A1685G mutant protein was markedly reduced compared to the wild type.

Conclusion: The present study indicated that the mutation might impair the secretion and synthesis of FXII protein, while the mutation only influences the secretion of FXII protein. The definition of these new mutations could be useful tools for analyzing the intracellular protein transport and structure-function relationship of FXII protein transport in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2021

Bi and Sb Codoped CsAgNaInCl Double Perovskite with Excitation-Wavelength-Dependent Dual-Emission for Anti-Counterfeiting Application.

ACS Appl Mater Interfaces 2021 Jul 23;13(26):31031-31037. Epub 2021 Jun 23.

Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, People's Republic of China.

The growing demands for optical anti-counterfeiting technology require the development of new environmentally friendly materials with single component, multimodal fluorescence and high stability. Herein, the Bi/Sb codoped CsAgNaInCl lead-free double perovskite material is reported as an efficient multimodal luminescence material with excitation-wavelength-dependent emission. When excited by 360 nm UV light, dual-emission is observed at 455 and 560 nm, which comes from the P-S transition of Sb ions and self-trapped excitons (STEs), respectively. Under the 320 nm UV lamp, the microcrystals show only a blue emission centered at 455 nm. Therefore, the Bi/Sb codoped CsAgNaInCl double perovskite emits blue and yellow lights under the 320 and 360 nm UV lamp, respectively. Moreover, the obtained double perovskite shows a high PLQY up to 41% and excellent stability against both air and high temperature, which make it a promising anti-counterfeiting material.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c07809DOI Listing
July 2021

Global-Local Multiple Granularity Learning for Cross-Modality Visible-Infrared Person Reidentification.

IEEE Trans Neural Netw Learn Syst 2021 Jun 17;PP. Epub 2021 Jun 17.

Cross-modality visible-infrared person reidentification (VI-ReID), which aims to retrieve pedestrian images captured by both visible and infrared cameras, is a challenging but essential task for smart surveillance systems. The huge barrier between visible and infrared images has led to the large cross-modality discrepancy and intraclass variations. Most existing VI-ReID methods tend to learn discriminative modality-sharable features based on either global or part-based representations, lacking effective optimization objectives. In this article, we propose a novel global-local multichannel (GLMC) network for VI-ReID, which can learn multigranularity representations based on both global and local features. The coarse- and fine-grained information can complement each other to form a more discriminative feature descriptor. Besides, we also propose a novel center loss function that aims to simultaneously improve the intraclass cross-modality similarity and enlarge the interclass discrepancy to explicitly handle the cross-modality discrepancy issue and avoid the model fluctuating problem. Experimental results on two public datasets have demonstrated the superiority of the proposed method compared with state-of-the-art approaches in terms of effectiveness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3085978DOI Listing
June 2021

Laser Synthesis and Microfabrication of Micro/Nanostructured Materials Toward Energy Conversion and Storage.

Nanomicro Lett 2021 Jan 4;13(1):49. Epub 2021 Jan 4.

Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, Institute for Advanced Interdisciplinary Research (iAIR), University of Jinan, Jinan, 250022, People's Republic of China.

Nanomaterials are known to exhibit a number of interesting physical and chemical properties for various applications, including energy conversion and storage, nanoscale electronics, sensors and actuators, photonics devices and even for biomedical purposes. In the past decade, laser as a synthetic technique and laser as a microfabrication technique facilitated nanomaterial preparation and nanostructure construction, including the laser processing-induced carbon and non-carbon nanomaterials, hierarchical structure construction, patterning, heteroatom doping, sputtering etching, and so on. The laser-induced nanomaterials and nanostructures have extended broad applications in electronic devices, such as light-thermal conversion, batteries, supercapacitors, sensor devices, actuators and electrocatalytic electrodes. Here, the recent developments in the laser synthesis of carbon-based and non-carbon-based nanomaterials are comprehensively summarized. An extensive overview on laser-enabled electronic devices for various applications is depicted. With the rapid progress made in the research on nanomaterial preparation through laser synthesis and laser microfabrication technologies, laser synthesis and microfabrication toward energy conversion and storage will undergo fast development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40820-020-00577-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187667PMC
January 2021
-->