Publications by authors named "Fan Li"

2,328 Publications

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[The Latest Research Progress on Myelodysplastic Syndrome Patient-derived Mesenchymal Stem Cell--Review].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Aug;30(4):1286-1290

Department of Hematology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Kunming 650500, Yunnan Province, China.

Myelodysplastic syndrome (MDS) are a heterogeneous group of hematological malignancies. Currently, in addition to demethylated chemotherapy and hematopoietic stem cell transplantation, MDS patient-derived mesenchymal stem cells (MDS-MSC) play an important role in understanding the pathogenesis of MDS and related therapeutic targets. For example, abnormal expression of DICER1 gene, abnormalities of PI3K/AKT and Wnt/β-catenin signaling pathways provide new therapeutic targets for MDS. In addition, MDS-MSC is also affected by abnormal microenvironment of the body, such as inflammatory factor S100A9, as well as hypercoagulation and iron overload. In this review, genes, signaling pathways, cytokines, hematopoietic microenvironment, and the effect of therapeutic drugs for MDS-MSC were briefly summarized.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.051DOI Listing
August 2022

Thoracic Imaging in China: Yesterday, Today, and Tomorrow.

J Thorac Imaging 2022 Aug 11. Epub 2022 Aug 11.

Second Affiliated Hospital, Naval Medical University.

Thoracic imaging has been revolutionized through advances in technology and research around the world, and so has China. Thoracic imaging in China has progressed from anatomic observation to quantitative and functional evaluation, from using traditional approaches to using artificial intelligence. This article will review the past, present, and future of thoracic imaging in China, in an attempt to establish new accepted strategies moving forward.
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http://dx.doi.org/10.1097/RTI.0000000000000670DOI Listing
August 2022

Assembly of a pangenome for global cattle reveals missing sequences and novel structural variations, providing new insights into their diversity and evolutionary history.

Genome Res 2022 Aug 17. Epub 2022 Aug 17.

Animal Genomics and Improvement Laboratory, BARC, USDA-ARS, Beltsville, Maryland 20705, USA.

A cattle pangenome representation was created based on the genome sequences of 898 cattle representing 57 breeds. The pangenome identified 83 Mb of sequence not found in the cattle reference genome, representing 3.1% novel sequence compared with the 2.71-Gb reference. A catalog of structural variants developed from this cattle population identified 3.3 million deletions, 0.12 million inversions, and 0.18 million duplications. Estimates of breed ancestry and hybridization between cattle breeds using insertion/deletions as markers were similar to those produced by single nucleotide polymorphism-based analysis. Hundreds of deletions were observed to have stratification based on subspecies and breed. For example, an insertion of a Bov-tA1 repeat element was identified in the first intron of the gene and correlated with cattle breed geographic distribution. This insertion falls within a segment overlapping predicted enhancer and promoter regions of the gene, and could affect important traits such as immune response, olfactory functions, cell proliferation, and glucose metabolism in muscle. The results indicate that pangenomes are a valuable resource for studying diversity and evolutionary history, and help to delineate how domestication, trait-based breeding, and adaptive introgression have shaped the cattle genome.
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http://dx.doi.org/10.1101/gr.276550.122DOI Listing
August 2022

A Retrospective Analysis of Outcome in MDA5-Related Interstitial Lung Disease Treated with Tofacitinib or Tacrolimus.

J Rheumatol 2022 Aug 15. Epub 2022 Aug 15.

Department of Pulmonary and Critical Care Medicine, Nanjing Drum Tower Hospital Clinical College of Jiangsu University, Nanjing, Jiangsu, China. Funding: The study was supported by the National Natural Science Foundation of China (81570058, 82170076), Jiangsu Provincial Medical Talent (ZDRCA2016058). Conflicts of interest: The authors have declared no conflicts of interest. Address Correspondence to: Prof. Yonglong Xiao, PhD, Department of Pulmonary and Critical Care Medicine, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing, 210008, China; E-mail: Prof. Jinghong Dai, PhD, Department of Pulmonary and Critical Care Medicine, Nanjing Drum Tower Hospital Clinical College of Jiangsu University, Nanjing, 210008, China; E-mail:

Objective: The efficacy of tofacitinib in early diagnosis of MDA5-ILD has been described. But whether tofacitinib exposure is associated with a reduced one-year mortality remains undetermined.

Methods: Patients diagnosed as MDA5-ILD receiving tofacitinib or tacrolimus treatment were included. Cox proportional hazards model adjusted for age, sex, smoking history, anti-MDA5 antibody titers, concurrent use of other steroids sparing agents was performed to compare all-cause mortality and to investigate the risk factors predicting 1-year mortality in the two treatment groups.

Results: During the study period, twenty-six patients were treated with tofacitinib and thirty-five with tacrolimus. The 6-month and 1-year mortality in tofacitinib group were significantly lower than those in tacrolimus group (38.5% vs. 62.9%, p=0.028; 44.0% vs. 65.7%, p=0.031, respectively). There were thirteen patients diagnosed as rapidly progressive-ILD (RPILD) in tofacitinib group and twenty-two in tacrolimus group. The majority of death occurred in patients with RPILD. The 6-month and 1-year mortality of patients with RPILD in tofacitinib group were also lower than those in tacrolimus group (76.9% and 95.5%, p=0.021; 84.6% and 100.0%, p=0.017). The adjusted model showed tofacitinib exposure was associated with a lower risk for 1-year mortality (HR0.438, 95% CI 0.200-0.960, p=0.039). While the incidence of adverse events and medication discontinuation rates between the two groups were similar (73.1% and 74.3%, p=1.000; 23.1% and 14.3%, p=0.504).

Conclusion: Our observational study showed tofacitinib use might have a potential impact on improving the outcomes of MDA5-ILD. Future clinical trials are needed to assess the long-term efficacy and tolerability of tofacitinib.
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http://dx.doi.org/10.3899/jrheum.220367DOI Listing
August 2022

Fujian cytoplasmic male sterility and the fertility restorer gene provide a promising breeding system for hybrid rice.

Proc Natl Acad Sci U S A 2022 Aug 15;119(34):e2208759119. Epub 2022 Aug 15.

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China.

Cytoplasmic male sterility (CMS) determined by mitochondrial genes and restorer of fertility () controlled by nuclear-encoded genes provide the breeding systems of many hybrid crops for the utilization of heterosis. Although several CMS/ systems have been widely exploited in rice, hybrid breeding using these systems has encountered difficulties due to either fertility instability or complications of two-locus inheritance or both. In this work, we characterized a type of CMS, Fujian Abortive cytoplasmic male sterility (CMS-FA), with stable sporophytic male sterility and a nuclear restorer gene that completely restores hybrid fertility. CMS is caused by the chimeric open reading frame that specifically occurs in the mitochondrial genome of CMS-FA rice. The restorer gene encodes a pentatricopeptide repeat (PPR) protein targeted to mitochondria, where it mediates the cleavage of transcripts, thus restoring male fertility. Comparative sequence analysis revealed that originated through a recent duplication in wild rice relatives, sharing a common ancestor with /, a fertility restorer gene for Boro II and Hong-Lian CMS. We developed six restorer lines by introgressing into parental lines of elite CMS-WA hybrids; hybrids produced from these lines showed equivalent or better agronomic performance relative to their counterparts based on the CMS-WA system. These results demonstrate that CMS-FA/ provides a highly promising system for future hybrid rice breeding.
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http://dx.doi.org/10.1073/pnas.2208759119DOI Listing
August 2022

Generalizing trial evidence to target populations in non-nested designs: Applications to AIDS clinical trials.

J R Stat Soc Ser C Appl Stat 2022 Jun 17;71(3):669-697. Epub 2022 Mar 17.

Department of Epidemiology, Gillings School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Comparative effectiveness evidence from randomized trials may not be directly generalizable to a target population of substantive interest when, as in most cases, trial participants are not randomly sampled from the target population. Motivated by the need to generalize evidence from two trials conducted in the AIDS Clinical Trials Group (ACTG), we consider weighting, regression and doubly robust estimators to estimate the causal effects of HIV interventions in a specified population of people living with HIV in the USA. We focus on a non-nested trial design and discuss strategies for both point and variance estimation of the target population average treatment effect. Specifically in the generalizability context, we demonstrate both analytically and empirically that estimating the known propensity score in trials does not increase the variance for each of the weighting, regression and doubly robust estimators. We apply these methods to generalize the average treatment effects from two ACTG trials to specified target populations and operationalize key practical considerations. Finally, we report on a simulation study that investigates the finite-sample operating characteristics of the generalizability estimators and their sandwich variance estimators.
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http://dx.doi.org/10.1111/rssc.12550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367209PMC
June 2022

The Effect of Routine Management Combined with Case Management Model on Social Support, Self-Efficacy, Self-Management Ability, and Psychological Flexibility of AIDS Patients.

Comput Math Methods Med 2022 4;2022:9213877. Epub 2022 Aug 4.

Department of Dermatology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, China.

Objective: To explore the influence of conventional management combined with case management on social support and self-efficacy of AIDS patients.

Methods: The clinical case data of 120 AIDS patients who were treated and nursed in our hospital from June 2019 to June 2021 were selected as the research objects and were divided into the control group and the observation group according to the digital table method, with 60 cases each. The control group implements routine management, and the observation group implements case-based nursing management on this basis and compares the effects of self-efficacy, self-management ability, nursing ability, social support, and psychological flexibility of the two groups of patients.

Results: Before the intervention, the quality of life scores of the two groups was not statistically significant ( > 0.05). After the intervention, the physical function score, pain management score, and symptom response score of the observation group were significantly higher than those of the control group, and statistics showed that the difference was statistically significant ( < 0.05). Before the intervention, the self-management ability of the two groups of patients was not statistically significant ( > 0.05). After the intervention, the observation group's symptom management, emotional cognition management, social support and assistance, daily life management, disease knowledge management, and treatment compliance management were significantly higher than those of the control group. Statistics show that this difference is statistically significant ( < 0.05). Before the intervention, there was no significant difference in the nursing ability and psychological flexibility between the two groups of patients ( > 0.05). After the intervention, the observation group's health knowledge level, self-care skills, self-care responsibility, self-concept, and mental flexibility (resilience, strength, optimism) indicators were higher than the control group, while the depression mood disorder score was significantly lower than the control group; statistics showed that this difference was statistically significant ( < 0.05).

Conclusion: Routine management combined with case-based nursing management can effectively improve the self-management ability and psychological flexibility of AIDS patients, improve patient care ability and self-efficacy, and provide certain reference value for effective management of AIDS patients.
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http://dx.doi.org/10.1155/2022/9213877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371859PMC
August 2022

Corrigendum: Effect of the Chinese New Year public holiday on the glycemic control of T1DM with intensive insulin therapy.

Front Endocrinol (Lausanne) 2022 25;13:985199. Epub 2022 Jul 25.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

[This corrects the article DOI: 10.3389/fendo.2022.915482.].
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http://dx.doi.org/10.3389/fendo.2022.985199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358356PMC
July 2022

Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials.

Nanomaterials (Basel) 2022 Aug 3;12(15). Epub 2022 Aug 3.

College of Textiles & Clothing, Qingdao University, Qingdao 266000, China.

Crude oil spills seriously harm the ocean environment and endanger the health of various animals and plants. In the present study, a totally biodegradable polymer, poly(L-lactic acid) (PLLA), was employed to fabricate highly porous oil absorbent nanofibrous materials by using a combination of electrospinning technique and subsequent acetone treatment. We systematically investigated how the electrospinning parameters affected formation of the porous structure of PLLA nanofibers and demonstrated that PLLA nanofibers with decreased and uniform diameter and improved porosity could be rapidly prepared by adjusting solution parameters and spinning parameters. We also demonstrated that the acetone treatment could obviously enhance the pore diameter and specific surface area of as-optimized electrospun PLLA nanofibers. The acetone treatment could also improve the hydrophobic property of as-treated PLLA nanofiber membranes. All these led to a significant increase in oil absorption performance. Through our research, it was found that the oil absorption of PLLA nanofiber membrane increased by more than double after being treated with acetone and the oil retention rate was also improved slightly.
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http://dx.doi.org/10.3390/nano12152670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370563PMC
August 2022

Production and characterization of novel thermo- and organic solvent-stable keratinase and aminopeptidase from Pseudomonas aeruginosa 4-3 for effective poultry feather degradation.

Environ Sci Pollut Res Int 2022 Aug 5. Epub 2022 Aug 5.

College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, People's Republic of China.

Feather biodegradation is an important premise for efficient resource development and utilization, in which keratinase plays an important role. However, there are few keratinases that combine the high activity, thermal stability, and organic solvent tolerance required for industrialization. This paper reported an efficient feather-degrading Pseudomonas aeruginosa 4-3 isolated from slaughterhouses. After 48 h of fermentation by P. aeruginosa 4-3 in a feather medium at 40 °C, pH 8.0, keratinase was efficiently produced (295.28 ± 5.42 U/mL) with complete feather degradation (95.3 ± 1.5%). Moreover, the keratinase from P. aeruginosa 4-3 showed high optimal temperature (55 °C), good thermal stability, wide pH tolerance, and excellent organic solvent resistance. In addition, P. aeruginosa 4-3-derived aminopeptidases also exhibit excellent thermal stability and organic solvent tolerance. Encouragingly, the reaction of crude keratinase and aminopeptidase with feathers for 8 h resulted in a 78% degradation rate of feathers. These properties make P. aeruginosa 4-3 keratinase and aminopeptidase ideal proteases for potential applications in keratin degradation, as well as provide ideas for the synergistic degradation of keratin by multiple enzymes.
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http://dx.doi.org/10.1007/s11356-022-22367-4DOI Listing
August 2022

Using Glycated Albumin and Stimulated C-Peptide to Define Partial Remission in Type 1 Diabetes.

Front Endocrinol (Lausanne) 2022 19;13:938059. Epub 2022 Jul 19.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Objective: To propose a new definition of partial remission (PR) for patients with type 1 diabetes (T1D) of all-ages using insulin dose and glycated albumin (GA), and find the optimal cut-off values for stimulated C-peptide to diagnose PR in different age-groups.

Research Design And Methods: Patients with newly diagnosed T1D (n=301) were included. GA/insulin dose was used to diagnose PR, and insulin dose-adjusted glycated albumin (IDAGA) was proposed to facilitate clinical application. The optimal diagnostic levels of IDAGA and stimulated C-peptide were determined in different age-groups (≤ 12y, 12-18y and ≥ 18y). Furthermore, the diagnostic consistency between different PR definitions was studied.

Results: GA≤ 23%/insulin dose ≤ 0.5u/kg/day was used to define PR, and IDAGA (GA (%) + 40 * insulin dose(u/kg/day)) ≤ 40 was feasible in all age-groups. Whereas, the optimal diagnostic level showed difference for stimulated C-peptide (265.5, 449.3 and 241.1 pmol/L for the ≤ 12y, 12-18y and ≥ 18y age-group, respectively). About 40% of patients met the PR definition by stimulated C-peptide but not GA/insulin dose or IDAGA, who showed dyslipidemia and higher insulin resistance.

Conclusions: A new definition of the PR phase is proposed using GA/insulin dose, and the calculated IDAGA≤ 40 applies to all age-groups. The stimulated C-peptide to diagnose PR is the highest in the 12-18y age-group, which reflects the effect of puberty on metabolism. For patients with insulin resistance, it is not recommended to use stimulated C-peptide alone to diagnose PR.
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http://dx.doi.org/10.3389/fendo.2022.938059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344919PMC
August 2022

Present and Future: Crosstalks Between Polycystic Ovary Syndrome and Gut Metabolites Relating to Gut Microbiota.

Front Endocrinol (Lausanne) 2022 19;13:933110. Epub 2022 Jul 19.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Polycystic ovary syndrome (PCOS) is a common disease, affecting 8%-13% of the females of reproductive age, thereby compromising their fertility and long-term health. However, the pathogenesis of PCOS is still unclear. It is not only a reproductive endocrine disease, dominated by hyperandrogenemia, but also is accompanied by different degrees of metabolic abnormalities and insulin resistance. With a deeper understanding of its pathogenesis, more small metabolic molecules, such as bile acids, amino acids, and short-chain fatty acids, have been reported to be involved in the pathological process of PCOS. Recently, the critical role of gut microbiota in metabolism has been focused on. The gut microbiota-related metabolic pathways can significantly affect inflammation levels, insulin signaling, glucose metabolism, lipid metabolism, and hormonal secretions. Although the abnormalities in gut microbiota and metabolites might not be the initial factors of PCOS, they may have a significant role in the pathological process of PCOS. The dysbiosis of gut microbiota and disturbance of gut metabolites can affect the progression of PCOS. Meanwhile, PCOS itself can adversely affect the function of gut, thereby contributing to the aggravation of the disease. Inhibiting this vicious cycle might alleviate the symptoms of PCOS. However, the role of gut microbiota in PCOS has not been fully explored yet. This review aims to summarize the potential effects and modulative mechanisms of the gut metabolites on PCOS and suggests its potential intervention targets, thus providing more possible treatment options for PCOS in the future.
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http://dx.doi.org/10.3389/fendo.2022.933110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343597PMC
August 2022

Robust muscle force prediction using NMFSEMD denoising and FOS identification.

PLoS One 2022 3;17(8):e0272118. Epub 2022 Aug 3.

Beijing Engineering Research Center of Industrial Spectrum Imaging, School of Automation and Electrical Engineering, University of Science and Technology Beijing, Beijing, China.

In this paper, an aliasing noise restraint technique and a system identification-based surface electromyography (sEMG)-force prediction model are proposed to realize a type of robust sEMG and muscle force prediction. For signal denoising, a novel non-negative matrix factorization screening empirical mode decomposition (NMFSEMD) and a fast orthogonal search (FOS)-based muscle force prediction model are developed. First, the NMFSEMD model is used to screen the empirical mode decomposition (EMD) results into the noisy intrinsic mode functions (IMF). Then, the noise matrix is computed using IMF translation and superposition, and the matrix is used as the input of NMF to obtain the denoised IMF. Furthermore, the reconstruction outcome of the NMFSEMD method can be used to estimate the denoised sEMG. Finally, a new sEMG muscle force prediction model, which considers a kind of candidate function in derivative form, is constructed, and a data-training-based linear weighted model is obtained. Extensive experimental results validate the suggested method's correction: after the NMFSEMD denoising of raw sEMG signal, the signal-noise ratio (SNR) can be improved by about 15.0 dB, and the energy percentage (EP) can be greater than 90.0%. Comparing with the muscle force prediction models using the traditional pretreatment and LSSVM, and the NMFSEMD plus LSSVM-based method, the mean square error (MSE) of our approach can be reduced by at least 1.2%.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272118PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348655PMC
August 2022

Is the product method more efficient than the difference method for assessing mediation?

Am J Epidemiol 2022 Aug 3. Epub 2022 Aug 3.

Department of Biostatistics, Yale School of Public Health, New Haven, CT.

Mediation analysis is widely used in biomedical research to quantify the extent to which a mediator may explain in exposure-outcome mechanisms. A traditional approach for quantifying mediation is through the difference method. The other popular approach uses a counterfactual framework from which the product method arises. However, there is little prior work to articulate which method is more efficient for estimating two key quantities in mediation analysis, the natural indirect effect (NIE) and mediation proportion (MP). To fill in this gap, we investigated the asymptotic relative efficiency for mediation measure estimators given by the product method and the difference method. We considered four data types characterized by continuous and binary mediatorsand outcomes. Under certain conditions, we show analytically that the product method is equally or more efficient than the difference method. However, our numerical studies demonstrate that the difference method is usually at least 90% efficient as the product method under realistic scenarios in epidemiological research, especially for estimating the MP. We demonstrate the efficiency results by analyzing the MaxART study, 2014-2017, which aims to evaluate the effectiveness of the early access to antiretroviral therapy among HIV-positive patients.
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http://dx.doi.org/10.1093/aje/kwac144DOI Listing
August 2022

Alcohol-soluble polysaccharides from Dendrobium officinale flowers as an antidepressant by regulating the gut-brain axis.

Int J Biol Macromol 2022 Sep 30;216:836-849. Epub 2022 Jul 30.

School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China. Electronic address:

This research aimed to explore the antidepressant components from Dendrobium officinale flowers (DOF) aqueous extract and their mechanism. Multiple depressive models were successfully used to show that alcohol-soluble polysaccharide-enriched fractions separated from DOF aqueous extract exhibited significant antidepressant effects. Subsequently, a novel alcohol-soluble polysaccharide named ASP was isolated, and its molecular mass was 3.10 × 10 Da. ASP was mainly composed of rhamnose, arabinose, fucose, mannose and glucose, and its glycosidic bond forms mainly included T-Manp, 1 → 2-linked Manp, T-Glcp, 1 → 3,6-linked Manp, 1 → 5-linked Araf, 1 → 6-linked Glcp. Microstructural observation of ASP displayed an irregular lamellar structure. Meanwhile, it was found that ASP could exhibit the significant antidepressant effect by modulating gut bacterial and fungal homeostasis and the levels of short-chain fatty acids to attenuate intestinal barrier disruption and excessive inflammatory responses, thus further achieving a protective effect on neuronal apoptosis and the maintenance of the 5-hydroxytryptamine system by activating the BDNF-TrkB-CREB pathway. Current findings outline using DOF as a rich source of a novel leading polysaccharide against depression.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.220DOI Listing
September 2022

Controllable Free Viewpoint Video Reconstruction Based on Neural Radiance Fields and Motion Graphs.

IEEE Trans Vis Comput Graph 2022 Aug 1;PP. Epub 2022 Aug 1.

In this paper, we propose a controllable high-quality free viewpoint video generation method based on the motion graph and neural radiance fields (NeRF). Different from existing pose-driven NeRF or time/structure conditioned NeRF works, we propose to first construct a directed motion graph of the captured sequence. Such a sequence-motion-parameterization strategy not only enables flexible pose control for free viewpoint video rendering but also avoids redundant calculation of similar poses and thus improves the overall reconstruction efficiency. Moreover, to support body shape control without losing the realistic free viewpoint rendering performance, we improve the vanilla NeRF by combining explicit surface deformation and implicit neural scene representations. Specifically, we train a local surface-guided NeRF for each valid frame on the motion graph, and the volumetric rendering was only performed in the local space around the real surface, thus enabling plausible shape control ability. As far as we know, our method is the first method that supports both realistic free viewpoint video reconstruction and motion graph-based user-guided motion traversal. The results and comparisons further demonstrate the effectiveness of the proposed method.
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http://dx.doi.org/10.1109/TVCG.2022.3192713DOI Listing
August 2022

Design and analysis of cluster randomized trials with time-to-event outcomes under the additive hazards mixed model.

Stat Med 2022 Jul 31. Epub 2022 Jul 31.

Department of Biostatistics, Yale University School of Public Health, New Haven, Connecticut, USA.

A primary focus of current methods for cluster randomized trials (CRTs) has been for continuous, binary, and count outcomes, with relatively less attention given to right-censored, time-to-event outcomes. In this article, we detail considerations for sample size requirement and statistical inference in CRTs with time-to-event outcomes when the intervention effect parameter is specified through the additive hazards mixed model (AHMM), which includes a frailty term to explicitly account for the dependency between the failure times. First, we discuss improved inference for the treatment effect parameter via bias-corrected sandwich variance estimators and randomization-based test under AHMM, addressing potential small-sample biases in CRTs. Next, we derive a new sample size formula for AHMM analysis of CRTs accommodating both equal and unequal cluster sizes. When the cluster sizes vary, our sample size formula depends on the mean and coefficient of variation of cluster sizes, based on which we articulate the impact of cluster size variation in CRTs with time-to-event outcomes. Furthermore, we obtain the insight that the classical variance inflation factor for CRTs with a non-censored outcome can in fact apply to CRTs with a time-to-event outcome, providing that an appropriate definition of the intraclass correlation coefficient is considered under AHMM. Simulation studies are carried out to illustrate key design and analysis considerations in CRTs with a small to moderate number of clusters. The proposed sample size procedure and analytical methods are further illustrated using the context of the STrategies to Reduce Injuries and Develop Confidence in Elders CRT.
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http://dx.doi.org/10.1002/sim.9541DOI Listing
July 2022

Electrospun kaolin-loaded chitosan/PEO nanofibers for rapid hemostasis and accelerated wound healing.

Int J Biol Macromol 2022 Jul 28;217:998-1011. Epub 2022 Jul 28.

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin 300457, PR China. Electronic address:

Development of chitosan-based hemostatic products and their application in wound healing has always been a research hotspot in the field of emergency treatment and biomedicine. In this study, the classic hemostatic chitosan and the most well-known inorganic hemostatic agent-kaolin were tried to combine to form a more excellent dressing. Together with the aid of non-toxic, harmless and good hydrophilic polyethylene oxide, chitosan/polyethylene oxide (PEO)/kaolin nanofiber membranes (CPKs) were prepared by electrospinning technology. Such membranes exhibited adjustable mechanical properties and good biocompatibility. Furthermore, a series of in vitro coagulation experiments proved that CPKs with 10 % ratio of kaolin (CPK10) has excellent hemostatic ability. Especially, in the whole blood coagulation time (WBCT) assay, the hemostatic time of CPK10 (43 ± 1.4 s) was significantly lower than that of chitosan/polyethylene oxide (CPK0) nanofiber membrane (61 ± 2.2 s) and QuikClot® Combat Gauze (55.7 ± 1.2 s). The further rat liver injury test reconfirmed that CPK10 can stop bleeding better and faster compared to other groups. In addition, CPKs could promote back wound healing in rats within 14 days without significant inflammatory response. This safe and effective hemostatic CPKs is expected to be a promising candidate hemostat in pre-hospital medical care.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.186DOI Listing
July 2022

Simple high-temperature annealing affords commercial carbon cloth with enhanced electrochemical performance for highly sensitive detection of imidacloprid.

J Pharm Biomed Anal 2022 Sep 25;219:114963. Epub 2022 Jul 25.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, PR China. Electronic address:

Imidacloprid (IDP) residue in modern agricultural production seriously endangers human health and environmental safety. The establishment of a rapid and efficient method for the detection of IDP residue can effectively prevent its harm to human health. Herein, we demonstrate the carbon cloth (CC) prepared by a high-temperature annealing strategy possesses enhanced electrochemical performance, which could be directly used in electrochemical IDP sensing. Annealed carbon cloth (ACC) is endowed with higher defects, rougher surfaces, more functional groups, more hydrophilic surface, and increased ion-accessible surface area. Furthermore, the ACC electrode shows superior electrocatalytic reduction activity towards IDP, possessing a wide linear range of 5-100 μM, a low detection limit of 0.04 μM, and high sensitivity of 35.58 μA mM cm. Meanwhile, this sensor can be applied for sensing IDP in grapes and apples with a good recovery of 96.8-104.1%. Compared with other modified electrodes, the ACC electrode has the advantages of no binder, no complicated modification, excellent detection effect, low cost, and easy large-scale production. Consequently, this work designs a self-supporting metal-free electrode with high electrochemical performance, providing a new idea for the development of environmentally friendly IDP sensors.
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http://dx.doi.org/10.1016/j.jpba.2022.114963DOI Listing
September 2022

Effects of translational motion on the Bjerknes forces of bubbles activated by strong acoustic waves.

Ultrasonics 2022 Jul 22;126:106809. Epub 2022 Jul 22.

Institute of Shaanxi Key Laboratory of Ultrasonics, Shaanxi Normal University, Xi'an 710119, China.

The motion of a small cavitation bubble is influenced by a neighboring large bubble, and these two bubbles can be mutually attractive or repulsive. The state of the bubble pair is strongly related to the secondary Bjerknes force in a strong acoustic field. Non-spherical oscillations are easily observed when a small cavitation bubble approaches a large bubble. The effects of translational motion and non-spherical oscillations of the small cavitation bubble on the secondary Bjerknes force are considered numerically in this paper, at large (greater than20 μm) and small (3 μm) bubble pair sizes. We show that the radial oscillation of the cavitation bubble is suppressed by the large bubble when the two are close to each other, and the magnitude and direction of the secondary Bjerknes force on the cavitation bubble are significantly different to those predicted results without considering translational motion. With the increase in size of the large bubble, the value of the secondary Bjerknes force varies from negative to positive, implying a transition between attraction and repulsion. The secondary Bjerknes force is sensitive to changes in the size of the large bubble, as there are many spikes in the curve in the range 20 μm < R < 100 μm. Moreover, the cavitation bubble is slightly repulsed when the large bubble is larger than 100 μm. There is a critical distance that can lead to a transition between mutual attraction and repulsion of bubbles. Cavitation bubbles can be driven toward the larger bubble by increasing acoustic pressure. The findings presented here will be helpful in understanding the complex behavior of cavitation bubbles in practical applications in which large bubbles exist and may interact with cavitation.
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http://dx.doi.org/10.1016/j.ultras.2022.106809DOI Listing
July 2022

Insights into the complicated networks contribute to adventitious rooting in transgenic MdWOX11 apple microshoots under nitrate treatments.

Plant Cell Environ 2022 Jul 28. Epub 2022 Jul 28.

College of Horticulture, Yangling Sub-Center of the National Center for Apple Improvement, Northwest A & F University, Yangling, Shaanxi, P. R. China.

Adventitious root formation is a bottleneck for the mass propagation of microshoots, and nitrate is an essential nutrient regulating adventitious roots. WOX11 is involved in adventitious rooting. But the crosstalk between nitrate and WOX11 is completely unknown. In this study, MdWOX11 transgenic apple microshoots were grown on different nitrate treatments. Low nitrate promotes adventitious rooting in overexpressed microshoots more than wild type and RNA interference microshoots. In contrast, medium nitrate significantly inhibits it in overexpressed and RNA interference microshoots compared with wild type microshoots. Stem anatomy indicated that medium nitrate delays root primordia formation compared with low nitrate. Methyl jasmonate and zeatin riboside played positive and negative roles in adventitious rooting, respectively. Transcriptomic analysis was conducted to understand the molecular mechanisms behind the phenotypes better. Hormone signalling, sugar metabolism, nitrogen metabolism, cell cycle and root development pathway-related genes were selected for their potential involvement in adventitious rooting. Results suggest that nitrogen signaling and MdWOX11 expression affect cytokinin accumulation and response to cytokinin through regulating the expression of genes related to cytokinin synthesis and transduction pathways, which ultimately affect adventitious rooting. This study provided important insights into the complicated networks involved in adventitious rooting in transgenic microshoots under nitrate treatments.
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http://dx.doi.org/10.1111/pce.14409DOI Listing
July 2022

The Thyroid Condition and Residual Clinical Signs in 31 Existing Endemic Neurological Cretins After 42 Years of Iodine Supplementation in China.

Front Endocrinol (Lausanne) 2022 8;13:911487. Epub 2022 Jul 8.

Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, China.

Backgroud: Endemic cretinism is the most severe manifestation among the iodine deficiency-related disorders. The clinical status of the cretins may be modified subsequently by the duration and severity of the disease. We aimed to reassess the clinical status and thyroid function of 31 surviving "neurological cretins" after 42 years of iodine supplementation in a historically severely iodine deficiency area of China.

Methods: It was a cross-sectional study in design and we investigated all 31 surviving neurological cretins and 85 controls. A detailed neurological examination was conducted on each patients. All the participants were given a questionnaire and underwent B-mode ultrasonography of the thyroid. The serum levels of thyroid hormones, thyroid antibodies, serum iodine concentration (SIC) and urine iodine concentration (UIC) were measured.

Results: The neurological cretins had shorter stature than that of the control. Neurological damage is still present in patients with cretinism. The prevalence of subclinical hypothyroidism and thyroid nodule in the cretins was significantly higher (4.766, =0.029 and 17.077, <0.0001, respectively) compared with the control. After adjusting for confounding factors, endemic neurocretinism was found to be an independent risk factor for subclinical hypothyroidism (OR=4.412; 95% CI: 1.358-14.334; =0.014) and thyroid nodule (OR=6.433; 95% CI: 2.323-17.816; <0.0001).

Conclusions: Iodine supplementation after birth does not reverse the neurological damage that results from maternal/foetal hypothyroidism and is subsequently manifested as neurological cretinism. There is a cross-sectional association between endemic neurocretinism and subclinical hypothyroidism and thyroid nodule.
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http://dx.doi.org/10.3389/fendo.2022.911487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309213PMC
July 2022

GRHL2 Expression Functions in Breast Cancer Aggressiveness and Could Serve as Prognostic and Diagnostic Biomarker for Breast Cancer.

Clin Med Insights Oncol 2022 21;16:11795549221109511. Epub 2022 Jul 21.

Department of Pathology, Tianjin Medical University, Tianjin, China.

Background: Breast cancer (BC) is the most frequent malignancy in women worldwide and the leading cause of female cancer-associated death in the world. Grainyhead-like 2 () is an important gene involved in human cancer progression. However, the role of GRHL2 in BC is unknown.

Methods: In this study, we used in vitro experiments to verify the role of expression in BC progression. We used 14 databases to analyse the expression level of GRHL2 in BC and its prognostic and diagnostic value. In addition, the correlation between GRHL2 expression and immune cell infiltration and DNA methylation was also analysed.

Results: At the cellular level, overexpression of induced E-cadherin expression in BC cells with a mesenchymal phenotype and resulted in a hybrid epithelial/mesenchymal (E/M) phenotype, which is more strongly correlated with tumour aggressiveness than a pure mesenchymal phenotype. Through analysis of various databases, we found that tumour tissue had a higher expression level of . High expression of was associated with worse prognosis of BC patients and indicated that GRHL2 had significant diagnostic value. Grainyhead-like 2 is also related to immune infiltration and regulated by DNA methylation. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses showed that -related signalling pathways in BC were related to tumour cell proliferation, invasion, and angiogenesis.

Conclusions: In summary, evidence indicates that can be used as a prognostic and diagnostic biomarker for BC.
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http://dx.doi.org/10.1177/11795549221109511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310218PMC
July 2022

A Secrecy Transmission Protocol with Energy Harvesting for Federated Learning.

Sensors (Basel) 2022 Jul 23;22(15). Epub 2022 Jul 23.

School of Information Engineering, Henan University of Science and Technology, Luoyang 471023, China.

In federated learning (FL), model parameters of deep learning are communicated between clients and the central server. To better train deep learning models, the spectrum resource and transmission security need to be guaranteed. Toward this end, we propose a secrecy transmission protocol based on energy harvesting and jammer selection for FL, in which the secondary transmitters can harvest energy from the primary source. Specifically, a secondary transmitter STi is first selected, which can offer the best transmission performance for the secondary users to access the primary frequency spectrum. Then, another secondary transmitter STn, which has the best channel for eavesdropping, is also chosen as a friendly jammer to provide secrecy service. Furthermore, we use outage probability (OP) and intercept probability (IP) as metrics to evaluate performance. Meanwhile, we also derive closed-form expressions of OP and IP of primary users and OP of secondary users for the proposed protocol, respectively. We also conduct a theoretical analysis of the optimal secondary transmission selection (OSTS) protocol. Finally, the performance of the proposed protocol is validated through numerical experiments. The results show that the secrecy performance of the proposed protocol is better than the OSTS and OCJS, respectively.
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http://dx.doi.org/10.3390/s22155506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371431PMC
July 2022

Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development.

Int J Mol Sci 2022 Jul 7;23(14). Epub 2022 Jul 7.

Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

It has been demonstrated that vascular endothelial growth factor B (VEGFB) and vascular endothelial growth factor receptor 1 (VEGFR1) play a vital role in regulating vascular biological function. However, the role of VEGFB and VEGFR1 in regulating fat deposition and skeletal muscle growth remains unclear. Therefore, this study was conducted to investigate the effects of VEGFB and VEGFR1 on fat deposition and skeletal muscle growth in mice. Our results showed that knockdown of VEGFB decreased body weight and iWAT index, stimulated the browning of mice iWAT with increased expression of UCP1, decreased the diameters of adipocytes, and elevated energy expenditure. In contrast, knockdown of VEGFB increased gastrocnemius (GAS) muscle index with increased proliferation of GAS muscle by expression of PCNA and Cyclin D1. Meanwhile, knockdown of endothelial VEGFR1 induced the browning of iWAT with increased expression of UCP1 and decreased diameters of adipocytes. By contrast, knockdown of endothelial VEGFR1 inhibited GAS muscle differentiation with decreased expression of MyoD. In conclusion, these results suggested that the loss of VEGFB/VEGFR1 signaling is associated with enhanced browning of inguinal white adipose tissue and skeletal muscle development. These results provided new insights into the regulation of skeletal muscle growth and regeneration, as well as fat deposition, suggesting the potential application of VEGFB/VEGFR1 as an intervention for the restriction of muscle diseases and obesity and related metabolic disorders.
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http://dx.doi.org/10.3390/ijms23147524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315609PMC
July 2022

Integrated analysis of phytochemical composition, pharmacokinetics, and network pharmacology to probe distinctions between the stems of and based on antidepressant activity.

Food Funct 2022 Aug 15;13(16):8542-8557. Epub 2022 Aug 15.

School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.

(CH), as a nutritional and functional supplement used in food and health care products for centuries, consists of the stems of and . Our previous studies confirmed that the stems of exerted advantageous antidepressant effect. However, whether the difference in the phytochemical compositions between the stems of and would lead to diverse bioavailability and accompanying antidepressant effects remain unclear, as well as their specific bioactive compounds and underlying mechanism. In this study, a series of comparative studies showed that the antidepressant activity of extract (CTE) was stronger than that of the extract (CDE), which was accompanied with the discovery of 10 differential markers from 52 identified compounds between CTE and CDE, and different pharmacokinetic behaviors of 9 prototype and 4 metabolites belonging to the glycosides between the CTE-treated and CDE-treated group in normal and depressive rats were simultaneously found by a validated UPLC-QTRAP-MS/MS method. Subsequently, network pharmacology prediction, and experiment verification from these differential markers further revealed that 7 compounds were confirmed to contribute to the antidepressant action of CH by inhibiting neuronal apoptosis mediated by mitochondrial function and activation of the AKT/GSK3β signaling pathway, synchronously indicating most of those, with higher bioavailability after CTE administration, that were responsible for the stronger antidepressant effect of CTE over CDE. Hence, the integrated analysis of phytochemical composition, pharmacokinetics, and network pharmacology provide new insights into the applications of CH from different botanical origins against depression.
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http://dx.doi.org/10.1039/d2fo01357fDOI Listing
August 2022

The genome of Eustoma grandiflorum reveals the whole-genome triplication event contributing to ornamental traits in cultivated lisianthus.

Plant Biotechnol J 2022 Jul 25. Epub 2022 Jul 25.

Floriculture Research Institute, Academy of Agricultural Sciences, National Engineering Research Center for Ornamental Horticulture, Key Laboratory for Flower Breeding of Yunnan Province, Yunnan, 650205, Kunming, China.

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http://dx.doi.org/10.1111/pbi.13899DOI Listing
July 2022

Noise-induced collective dynamics in the small-world network of photosensitive neurons.

J Biol Phys 2022 Jul 25. Epub 2022 Jul 25.

College of Energy Engineering, Yulin University, Yulin, 719000, People's Republic of China.

Photosensitive neurons can capture and convert external optical signals, and then realize the encoding signal. It is confirmed that a variety of firing modes could be induced under optical stimuli. As a result, it is interesting to explore the mode transitions of collective dynamics in the photosensitive neuron network under external stimuli. In this work, the collective dynamics of photosensitive neurons in a small-world network with non-synaptic coupling will be discussed with spatial diversity of noise and uniform noise applied on, respectively. The results prove that a variety of different collective electrical activities could be induced under different conditions. Under spatial diversity of noise applied on, a chimera state could be observed in the evolution, and steady cluster synchronization could be detected in the end; even the nodes in each cluster depend on the degree of each node. Under uniform noise applied on, the complete synchronization window could be observed alternately in the transient process, and steady complete synchronization could be detected finally. The potential mechanism is that continuous energy is pumped in the phototubes, and energy exchange and balance between neurons to form the resonance synchronization in the network with different noise applied on. Furthermore, it is confirmed that the evolution of collective dynamical behaviors in the network depends on the external stimuli on each node. Moreover, the bifurcation analysis for the single neuron model is calculated, and the results confirm that the electrical activities of single neuron are sensitive to different kinds of noise.
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http://dx.doi.org/10.1007/s10867-022-09610-2DOI Listing
July 2022

Relationships between the serum TPOAb and TGAb antibody distributions and water iodine concentrations, thyroid hormones and thyroid diseases: a cross-sectional study of 2503 adults in China.

Br J Nutr 2022 Jul 25:1-11. Epub 2022 Jul 25.

Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang150081, China.

The aim of this study was to explore the status of thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TGAb) in three areas with differing water iodine concentrations; and to discuss the relationships between these two thyroid antibodies and thyroid diseases in the three areas. We investigated 2503 adults from three areas. Urinary iodine concentrations, thyroid stimulating hormone (TSH), free thyroxine (FT), free triiodothyronine (FT), TPOAb, TGAb and thyroid volume (TV) were measured, and thyroid ultrasonography was performed. The positivity rates of TGAb(+), TPOAb(+) and TGAb(+) and TPOAb(+) or TGAb(+) were significantly higher in iodine fortification (IF) areas than iodine adequate (IA) areas (all < 0·05). In IF and iodine excess areas, the positivity rates of TPOAb(+), TGAb(+) and TPOAb(+) or TGAb(+) significantly increased with age (all for trend < 0·05). The levels of TSH, TV and the prevalence of overt hypothyroidism, subclinical hypothyroidism and goitre were significantly elevated in the thyroid antibody-positive groups in the three areas, but the FT was diminished (all < 0·010). Positivity for TPOAb and TGAb was associated with an increased risk of subclinical hypothyroidism in the three areas. In areas with different median water iodine, positivity for both TPOAb and TGAb was associated with elevated TSH values. Notably, with the increased levels of TPOAb, the frequency of abnormally elevated TSH increased dramatically in the three areas.
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http://dx.doi.org/10.1017/S0007114522002367DOI Listing
July 2022

Bowen disease of the nail unit associated with HPV-16 infection in a pemphigus patient following rituximab treatment

Eur J Dermatol 2022 04;32(2):270-272

Department of Dermatology, West China Hospital, Sichuan University, No. 37 Guoxue Street, Chengdu, Sichuan, 610041, China

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http://dx.doi.org/10.1684/ejd.2022.4226DOI Listing
April 2022
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