Publications by authors named "Fabíola de Souza Rodrigues"

2 Publications

  • Page 1 of 1

Maintenance of venomous snakes in captivity for venom production at Butantan Institute from 1908 to the present: a scoping history.

J Venom Anim Toxins Incl Trop Dis 2021 22;27:e20200068. Epub 2021 Jan 22.

Laboratory of Herpetology, Butantan Institute, São Paulo, SP, Brazil.

Maintenance of snakes at Butantan Institute started in the last century, intending to produce a different antivenom serum to reduce death caused by snakebites. Through a successful campaign coordinated by Vital Brazil, farmers sent venomous snakes to Butantan Institute by the railway lines with no cost. From 1908 to 1962, the snakes were kept in an outdoor serpentarium, where venom extraction was performed every 15 days. During this period, the snake average survival was 15 days. In 1963, the snakes were transferred to an adapted building, currently called Laboratory of Herpetology (LH), to be maintained in an intensive system. Although the periodicity of venom extraction remained the same, animal average survival increased to two months. With the severe serum crisis in 1983, the Ministry of Health financed remodeling for the three public antivenom producers, and with this support, the LH could be improved. Air conditioning and exhausting systems were installed in the rooms, besides the settlement of critical hygienic-sanitary managements to increase the welfare of snakes. In the early 1990s, snake survival was ten months. Over the years to the present day, several improvements have been made in the intensive serpentarium, as the establishment of two quarantines, feeding with thawed rodents, an interval of two months between venom extraction routines, and monitoring of snake health through laboratory tests. With these new protocols, average snake survival increased significantly, being eight years for the genus , ten years for genus and and four years for the genus . Aiming the production of venoms of good quality, respect for good management practices is essential for the maintenance of snakes in captivity. New techniques and efficient management must always be sought to improve animal welfare, the quality of the venom produced, and the safety of those working directly with the venomous snakes.
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http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856910PMC
January 2021

Ontogenetic study of Bothrops jararacussu venom composition reveals distinct profiles.

Toxicon 2020 Oct 5;186:67-77. Epub 2020 Aug 5.

Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil; Interunidades em Biotecnologia, Universidade de São Paulo, Instituto de Pesquisas Tecnológicas, Instituto Butantan, São Paulo, Brazil. Electronic address:

Concerning snake venoms, numerous authors worked with different species of Bothrops focusing on the ontogeny of these animals. However, according to PubMed database, no results on studies related to Bothrops jararacussu ontogeny were displayed until now. This fact led us to develop a greater interest in the venom ontogenetic variability of this species, which is little explored so far. Among snakes of the genus Bothrops, B. jararacussu was previously described as the one with highest myotoxic activity. Another peculiarity was also observed in its venom: a low rate of immunogenicity. In addition, its activity is not efficiently neutralized by the specific antibothropic serum. Considering these particularities, we performed an ontogenetic study of B. jararacussu using venom samples from newborns of the same litter (<6 months) and adults (>24 months). Our results identified two distinct profiles in the venom of these animals: young individuals with little PLA K-49 and more proteases; and adults with a lot of the same myotoxic PLA but less proteases. The HPLC and SDS-PAGE profiles corroborated our findings. Adults showed more hemorrhagic activity in vivo than juveniles, while adult males showed less activity when compared to females. In vivo myotoxicity activity was higher in adults than in juveniles. Immune recognition assays showed different results for the distinct venom.
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http://dx.doi.org/10.1016/j.toxicon.2020.07.030DOI Listing
October 2020