Publications by authors named "F L Oliveira"

2,063 Publications

  • Page 1 of 1

Caudal Extension Graft of the Lower Lateral Cartilage: Technique and Aesthetic and Functional Results.

Facial Plast Surg 2021 Apr 14. Epub 2021 Apr 14.

Pontificia Universidade Catolica de Minas Gerais, Medical School, Belo Horizonte, Minas Gerais, Brazil.

Here we describe a new technique to deal with alar retraction, a highly undesirable imperfection of the nose. The procedure involves placing a caudal extension graft below the vestibular portion of the lower lateral cartilage (LLC) after its detachment from the vestibular skin. The graft is fixed to the cartilage and, subsequently, to the vestibular tissue. The present retrospective study included 20 patients, 11 females and 9 males, with a mean age of 28.90 years. Follow-up ranged from 1 to 18 months. Surgery improved alar notching to a smoother dome shape and nostril exposure was reduced in every patient. The caudal extension graft of the LLC contributed to rise in overall patient satisfaction, as revealed by the postoperative increase of the Rhinoplasty Outcomes Evaluation (ROE) mean score from 40.0 to 79.17 ( < 0.0001). It also contributed to and improved functional outcomes, as indicated by the decrease of the Nasal Obstruction Symptom Evaluation (NOSE) mean score from 52.75 to 13.25 ( = 0.0001). Sex did not affect the mean ROE and NOSE scores. Thus, increased patient satisfaction measured by the ROE is present in both sexes and at both age groups but it is better detected in the first year after surgery. Functional improvements analyzed with NOSE are best detected in patients aged ≥ 30 years and in follow-ups of 11 months. The caudal extension graft of the LLC technique described herein effectively and safely corrects alar retraction and the collapse of the nasal valve while filling the soft triangle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0041-1726025DOI Listing
April 2021

Two cases of neural tube defects with dolutegravir use at conception in south Brazil.

Braz J Infect Dis 2021 Apr 11:101572. Epub 2021 Apr 11.

Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil; Federal University of Rio Grande do Sul, School of Medicine, Porto Alegre, RS, Brazil. Electronic address:

Dolutegravir (DTG) is amongst the most prescribed antiretrovirals worldwide and is recommended as first line regimen in most HIV treatment guidelines. Its use, although infrequently, had been associated to an increased chance of neural tube defects (NTD) in Botswana, Africa. Herein we describe two cases of NTD in women who conceived while taking DTG as part of their antiretroviral treatment in the city of Porto Alegre, Brazil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bjid.2021.101572DOI Listing
April 2021

Automatic classification of idiopathic Parkinson's disease and atypical parkinsonian syndromes combining [C]raclopride PET uptake and MRI grey matter morphometry.

J Neural Eng 2021 Apr 13. Epub 2021 Apr 13.

Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT), ICNAS Polo 3, Coimbra, 3000-548, PORTUGAL.

Objective: To explore the viability of developing a computer-aided diagnostic system for Parkinsonian syndromes using dynamic [11C]raclopride PET and T1-weighted MRI data.

Approach: The biological heterogeneity of Parkinsonian syndromes renders their statistical classification a challenge. The unique combination of structural and molecular imaging data allowed different classifier designs to be tested. Datasets from dynamic [11C]raclopride PET and T1-weighted MRI scans were acquired from six groups of participants: healthy controls (CTRL n=15), patients with Parkinson's disease (PD n=27), multiple system atrophy (MSA n=8), corticobasal degeneration (CBD n=6), and dementia with Lewy bodies (DLB n=5). MSA, CBD, and DLB patients were classified into one category designated as atypical parkinsonism (AP). The distribution volume ratio (DVR) kinetic parameters obtained from PET data were used to quantify the reversible tracer binding to D2/D3 receptors in the subcortical regions of interest (ROI). Grey matter (GM) volumes obtained from the MRI data were used to quantify GM atrophy across cortical, subcortical, and cerebellar ROI.

Results: The classifiers CTRL vs PD and CTRL vs AP achieved the highest balanced accuracy combining DVR and GM (DVR-GM) features (96.7%, 92.1%, respectively), followed by the classifiers designed with DVR features (93.3%, 88.8%, respectively), and GM features (69.6%, 86.1%, respectively). In contrast, the classifier PD vs AP showed the highest balanced accuracy (78.9%) using DVR features only. The integration of DVR-GM (77.9%) and GM features (72.7%) produced inferior performances. The classifier CTRL vs PD vs AP showed high weighted balanced accuracy when DVR (80.5%) or DVR-GM features (79.9%) were integrated. GM features revealed poorer performance (59.5%).

Significance: This work was unique in its combination of structural and molecular imaging features in binary and triple category classifications. We were able to demonstrate improved binary classification of healthy/diseased status (concerning both PD and AP) and equate performance to DVR features in multiclass classifications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1741-2552/abf772DOI Listing
April 2021

Use of robotic assistance to reduce proximity and air-sharing during percutaneous cardiovascular intervention.

Future Cardiol 2021 Apr 9. Epub 2021 Apr 9.

Hospital Israelita Albert Einstein, Sao Paulo-SP, Brazil.

Traditional percutaneous cardiovascular interventions require close physical proximity between the patients and the healthcare team, posing occupational hazards that range from radiation exposure to interpersonal air contamination. Prospective single-arm pilot study (n = 10) to investigate robotic-assisted intervention as a strategy to reduce proximity during the procedure. Primary end point: composite of angiographic success, intervention performed with the team positioned >2 meters from the patient for ≥50% procedure duration, and absence of in-hospital death or acute target lesion occlusion. The composite primary end point was achieved in 100% of cases. Robotic-assisted percutaneous intervention provided successful invasive treatment while reducing proximity and shared air space between the care-delivery team and the patient during the procedure. Trial registration number: NCT04379453 (Clinicaltrials.gov).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2217/fca-2021-0024DOI Listing
April 2021

Streptococcus mutans in atherosclerotic plaque: molecular and immunohistochemical evaluations.

Oral Dis 2021 Apr 7. Epub 2021 Apr 7.

Department of Stomatology and Oral Pathology, School of Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.

Objectives: To verify the presence of Streptococcus mutans (SM) in atherosclerotic plaque (AP) using techniques with different sensitivities, correlating with histological changes in plaque and immunoexpression of inflammatory markers.

Materials And Methods: Thirteen AP samples were subjected to real-time polymerase chain reaction (qRT-PCR), histopathological analyses, histochemical analysis by Giemsa staining (GS), and immunohistochemical analysis for SM, IL-1β, and TNF-α (streptavidin-biotin-peroxidase method). Ten necropsy samples of healthy vessels were used as controls.

Results: All AP samples showed histopathological characteristics of severe atherosclerosis and were positive for SM (100.0%) in qRT-PCR and immunohistochemical analyses. GS showed that Streptococcus sp. colonized the lipid-rich core regions and fibrous tissue, while the control group was negative for Streptococcus sp. IL-1β and TNF-α were expressed in 100% and 92.3% of the AP tested, respectively. The control samples were positive for SM in qRT-PCR analysis, but negative for SM, IL-1β, and TNF-α in immunohistochemical analyses.

Conclusion: The detection of SM in AP and the visualization of Streptococcus sp. suggested a possible association between SM and atherosclerosis. The results obtained from the control samples suggested the presence of DNA fragments or innocuous bacteria that were not associated with tissue alteration. However, future studies are necessary to provide more information.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/odi.13869DOI Listing
April 2021

CO2 Capture by Hydroxylated Azine-Based Covalent Organic Frameworks.

Chemistry 2021 Apr 3. Epub 2021 Apr 3.

Federal University of Rio de Janeiro: Universidade Federal do Rio de Janeiro, Instituto de Quimica, BRAZIL.

Covalent Organic Frameworks RIO-13, RIO-12, RIO-11, and RIO-11m were investigated towards their CO2 capture properties by thermogravimetric analysis at 1 atm and 40 °C. These microporous COFs bear in common the azine backbone composed of hydroxy-benzene moieties but differ in the relative number of hydroxyl groups present in each material. Thus, their sorption capacities were studied as a function of their textural and chemical properties. Their maximum CO2 uptake values showed a strong correlation with an increasing specific surface area, but that property alone could not fully explain the CO2 uptake data. Hence, the specific CO2 uptake, combined with DFT calculations, indicated that the relative number of hydroxyl groups in the COF backbone acts as an adsorption threshold, as the hydroxyl groups were indeed identified as relevant adsorption sites in all the studied COFs. Additionally, the best performing COF was thoroughly investigated, experimentally and theoretically, for its CO2 capture properties in a variety of CO2 concentrations and temperatures, and showed excellent isothermal recyclability up to 3 cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.202100478DOI Listing
April 2021

First report of costus stripe mosaic virus infecting Tradescantia spathacea plants in Brazil.

Plant Dis 2021 Mar 31. Epub 2021 Mar 31.

Universidade de São Paulo - ESALQ, Fitopatologia e Nematologia, Av. Padua Dias 11, Piracicaba, São Paulo, Brazil, 13418-900;

Tradescantia spathacea (family Commelinaceae) is cultivated worldwide as an ornamental (Golczyk et al., 2013) and as medicinal plant (Tan et al., 2020). In 2019, 90 of ~180 plants of T. spathacea, grown in two beds of 4 m2 and exhibiting leaf mosaic were found in an experimental area at ESALQ/USP (Piracicaba municipality, São Paulo state, Brazil). Potyvirus-like flexuous filamentous particles were observed by transmission electron microscopy in foliar extracts of two symptomatic plants stained with 1% uranyl acetate. Total RNA was extracted using the Purelink viral RNA/DNA kit (Thermo Fisher Scientific) from leaves of two symptomatic plants and separately subjected to a reverse transcription polymerase chain reaction (RT-PCR). The potyviruses degenerate pairs of primers CIFor/CIRev (Ha et al. 2008), which amplifies a fragment corresponding to part of the cylindrical inclusion protein gene, and WCIEN/PV1 (Maciel et al. 2011), which amplifies a fragment containing part of the capsid protein gene and the 3' untranslated region, were used. The expected amplicons (~700bp) were obtained from both total RNA extracts. Two amplicons from one sample were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) and directly sequenced in both directions at Macrogen Inc (Seoul, South Korea). The obtained nucleotide sequences (GenBank MW430005 and MW503934) shared 95.32% and 97.79% nucleotide identity, respectively, with the corresponding sequences of the Brazilian isolate of the potyvirus costus stripe mosaic virus (CoSMV, MK286375) (Alexandre et al. 2020). Extract from an infected plant of T. spathacea was mechanically inoculated in 10 healthy plants of T. spathacea and two plants each of the following species: Capsicum annuum, Chenopodium amaranticolor, Commelina benghalensis, Datura stramonium, Gomphrena globosa, Nicandra physaloides, Nicotiana tabacum cvs. Turkish and Samsun, Solanum lycopersicum, T. palida, and T. zebrina. All T. spathacea plants exhibited mosaic and severe leaf malformation. C. benghalensis plants developed mild mosaic, whereas infected T. zebrina plants were asymptomatic. The plants of other species were not infected. RT-PCR with specific CoSMV primers CoSMVHC-F and CoSMVHC-R (Alexandre et al. 2020) confirmed the infection. Nucleotide sequences of amplicons obtained from experimentally inoculated T. spathacea and T. zebrina (MW430007 and MW430008) shared 94.56% and 94.94% identity with the corresponding sequence of a Brazilian CoSMV isolate (MK286375). None of eight virus-free plants of T. spathacea inoculated with CoSMV using Aphis craccivora exhibited symptoms, nor was CoSMV detected by RT-PCR. Lack of CoSMV transmission by A. solanella, Myzus persicae, and Uroleucon sonchi was previously reported (Alexandre et al. 2020). T. spathacea plants are commonly propagated vegetatively, and by seeds. Virus-free seeds, if available, can provide an efficient and easy way to obtain healthy plants. Only three viruses were reported in plants of the genus Tradescantia: Commelina mosaic virus, tradescantia mild mosaic virus, and a not fully characterized potyvirus (Baker and Zettler, 1988; Ciuffo et al., 2006; Kitajima 2020). CoSMV was recently reported infecting Costus spiralis and C. comosus (Alexandre et al. 2020). As far as we know, this is the first report of CoSMV infecting T. spathacea plants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-02-21-0342-PDNDOI Listing
March 2021

Potential autoimmune encephalitis following yellow fever vaccination: A report of three cases.

J Neuroimmunol 2021 Mar 17;355:577548. Epub 2021 Mar 17.

Departmento de Neurologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.

Meningoencephalitis following yellow fever vaccination is considered a viral neuroinvasive disease. We describe three patients with typical autoimmune encephalitis syndromes that developed 1-27 days following yellow fever vaccination. Anti-N-methyl-d-aspartate-r antibodies were identified in the CSF and serum of two patients and the other case was associated with anti-neurexin-3 antibodies. One case was confirmed as vaccine-associated neurotropic disease due to reactive CSF yellow fever IgM, which suggested an infectious-autoimmune overlap mechanism. Two aditional cases of Anti-N-methyl-d-aspartate-r encephalitis were identified in the literature review. Antibody-positive autoimmune encephalitis should be included in the differential diagnosis of neurologic adverse events following yellow fever vaccination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jneuroim.2021.577548DOI Listing
March 2021

Pharmacological approaches to induce follicular growth and ovulation for fixed-time artificial insemination treatment regimens in ewes.

Anim Reprod Sci 2021 Mar 9;228:106734. Epub 2021 Mar 9.

ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil; Faculdade de Veterinária, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil. Electronic address:

In Experiment I, during the non-breeding season, after intravaginal devices containing progesterone (P) were withdrawn (n = 28), estrous rates were greater with treatment with 400 IU eCG (P <  0.05) than with FSH (10 and 15 mg) and no treatment. During the breeding season (n = 147), estrous and pregnancy rates after fixed-time artificial inseminations (FTAI) were similar among groups: 300 IU eCG; 10 mg FSH; and control (P >  0.05). In Experiment II (non-breeding season), ewes of one group were treated with 300 IU eCG (n = 8) and of two groups were treated with 10 mg FSH. In one FSH group, 250 μg estradiol benzoate (EB) were administered after 24 h (n = 9); in the other, 4 μg GnRH were administered after 36 h (n = 10). Serum P concentrations were greater in eCG-treated ewes (P < 0.05). Estrous rates were similar for the eCG- and FSH plus EB-treated ewes (P >  0.05). In Experiment III (breeding season), the treatments were: 300 IU eCG; 250 μg estradiol cypionate; 250 μg EB; or control (n = 22). Follicular growth was greater for eCG-treated ewes within 0-24 h and for control ewes within 48-72 h (P =  0.001). Although estrous and ovulation rates did not differ (P >  0.05), all eCG-treated ewes had ovulations. During the non-breeding season, FSH treatment promoted follicular growth but did not induce ovulations. For FTAI regimens, eCG was more effective than FSH plus GnRH and estradiol esters in inducing estrus and ovulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.anireprosci.2021.106734DOI Listing
March 2021

Case Report: A Severe SARS-CoV-2 Infection in a Teenager With Angelman Syndrome.

Front Med (Lausanne) 2021 12;8:629112. Epub 2021 Mar 12.

Hospital Sírio-Libanês, São Paulo, Brazil.

Teenagers generally present mild to no symptoms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present report, we present the case of a 14-year-old boy with Angelman syndrome (AS) who presented with severe COVID-19 symptoms. He spent 20 days in the ICU with elevated inflammatory biomarkers (C-reactive protein and D-dimer) and increased peaks of neutrophil-to-lymphocyte ratio, which is uncommon for teenagers diagnosed with COVID-19. Although he showed physiological instability, he was able to produce neutralizing antibodies, suggesting a functional immune response. The literature concerning the immune response to infections in patients with AS is still poor, and to our knowledge, this was the first report of a patient with AS diagnosed with COVID-19. As such, the present study may alert other patients with AS or other rare diseases that they lack a competent immune response and could suffer severe consequences of SARS-CoV-2 infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.629112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994262PMC
March 2021

Immunoinformatics Design of Multi-Epitope Peptide-Based Vaccine Against Using Transmembrane Proteins as a Target.

Front Immunol 2021 2;12:621706. Epub 2021 Mar 2.

Laboratório de Biologia Molecular, Universidade Federal de São João del-Rei, Divinópolis, Brazil.

Schistosomiasis remains a serious health issue nowadays for an estimated one billion people in 79 countries around the world. Great efforts have been made to identify good vaccine candidates during the last decades, but only three molecules reached clinical trials so far. The reverse vaccinology approach has become an attractive option for vaccine design, especially regarding parasites like spp. that present limitations for culture maintenance. This strategy also has prompted the construction of multi-epitope based vaccines, with great immunological foreseen properties as well as being less prone to contamination, autoimmunity, and allergenic responses. Therefore, in this study we applied a robust immunoinformatics approach, targeting transmembrane proteins, in order to construct a chimeric antigen. Initially, the search for all hypothetical transmembrane proteins in GeneDB provided a total of 584 sequences. Using the PSORT II and CCTOP servers we reduced this to 37 plasma membrane proteins, from which extracellular domains were used for epitope prediction. Nineteen common MHC-I and MHC-II binding epitopes, from eight proteins, comprised the final multi-epitope construct, along with suitable adjuvants. The final chimeric multi-epitope vaccine was predicted as prone to induce B-cell and IFN-γ based immunity, as well as presented itself as stable and non-allergenic molecule. Finally, molecular docking and molecular dynamics foresee stable interactions between the putative antigen and the immune receptor TLR 4. Our results indicate that the multi-epitope vaccine might stimulate humoral and cellular immune responses and could be a potential vaccine candidate against schistosomiasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.621706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961083PMC
March 2021

Diversity of Fungi Present in Permafrost in the South Shetland Islands, Maritime Antarctic.

Microb Ecol 2021 Mar 17. Epub 2021 Mar 17.

Laboratório de Microbiologia Polar e Conexões Tropicais, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, P. O. Box 486, Belo Horizonte, MG, CEP 31270-901, Brazil.

We assess the fungal diversity present in permafrost from different islands in the South Shetland Islands archipelago, maritime Antarctic, using next-generation sequencing (NGS). We detected 1,003,637 fungal DNA reads representing, in rank abundance order, the phyla Ascomycota, Mortierellomycota, Basidiomycota, Chytridiomycota, Rozellomycota, Mucoromycota, Calcarisporiellomycota and Zoopagomycota. Ten taxa were dominant these being, in order of abundance, Pseudogymnoascus appendiculatus, Penicillium sp., Pseudogymnoascus roseus, Penicillium herquei, Curvularia lunata, Leotiomycetes sp., Mortierella sp. 1, Mortierella fimbricystis, Fungal sp. 1 and Fungal sp. 2. A further 38 taxa had intermediate abundance and 345 were classified as rare. The total fungal community detected in the permafrost showed high indices of diversity, richness and dominance, although these varied between the sampling locations. The use of a metabarcoding approach revealed the presence of DNA of a complex fungal assemblage in the permafrost of the South Shetland Islands including taxa with a range of ecological functions among which were multiple animal, human and plant pathogenic fungi. Further studies are required to determine whether the taxa identified are present in the form of viable cells or propagules and which might be released from melting permafrost to other Antarctic habitats and potentially dispersed more widely.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00248-021-01735-6DOI Listing
March 2021

Inhibition of nitric oxide production of activated mice peritoneal macrophages is independent of the Toxoplasma gondii strain.

Mem Inst Oswaldo Cruz 2021 10;116:e200417. Epub 2021 Mar 10.

Universidade Estadual do Norte Fluminense, Centro de Biociências e Biotecnologia, Laboratório de Biologia Celular e Tecidual, Campos dos Goytacazes, RJ, Brasil.

Background: Toxoplasma gondii causes toxoplasmosis and is controlled by activated macrophages. However, infection of macrophages by tachyzoites induces TGF-β signaling (TGF-s) inhibiting nitric oxide (NO) production. NO inhibition may be a general escape mechanism of distinct T. gondii strains.

Objectives: To evaluate in activated macrophages the capacity of T. gondii strains of different virulence and genetics (RH, type I; ME-49, type II; VEG, type III; P-Br, recombinant) to evade the NO microbicidal defense system and determine LC3 loading to the parasitophorous vacuole.

Methods: Activated peritoneal macrophages were infected with the different T. gondii strains, NO-production was evaluated by the Griess reagent, and inducible nitric oxide synthase expression, TGF-s, and LC3 localisation assayed by immunofluorescence.

Findings: Only RH persisted in macrophages, while VEG was more resistant than P-Br and ME-49. All strains induced TGF-s, degradation of inducible nitric oxide synthase, and NO-production inhibition from 2 to 24 h of infection, but only RH sustained these alterations for 48 h. By 24 h of infection, TGF-s lowered in macrophages infected by ME-49, and P-Br, and NO-production recovered, while VEG sustained TGF-s and NO-production inhibition longer. LC3 loading to parasitophorous vacuole was strain-dependent: higher for ME-49, P-Br and VEG, lower for RH. All strains inhibited NO-production, but only RH sustained this effect probably because it persisted in macrophages due to additional evasive mechanisms as lower LC3 loading to parasitophorous vacuole.

Main Conclusions: These results support that T. gondii can escape the NO microbicidal defense system at the initial phase of the infection, but only the virulent strain sustain this evasion mechanism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/0074-02760200417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949196PMC
March 2021

Kidney transplantation in the time of COVID-19: Dilemmas, experiences, and perspectives.

Transpl Infect Dis 2021 Mar 17:e13600. Epub 2021 Mar 17.

Transplant Division, Hospital do Rim, UNIFESP, São Paulo, Brazil.

Introduction: Hospital do Rim is a high-volume kidney transplant (KT) center located in São Paulo, a city with 12.2 million inhabitants. Over the last 18 years, we performed 11 436 KT, 70% of which from deceased donors. To mitigate the effects of reduction in the number of transplants on the waiting list, sequential measures were implemented when COVID-19 was declared pandemic.

Methods: The first step was to provide SARS-COV-2 RT-PCR testing for all symptomatic employees and patients and the compulsory use of personal protective equipment in the hospital facilities. Living donor KT were postponed, and all deceased donors and recipients were tested before the transplantation. The immunosuppressive protocols were maintained, and telehealth strategies were developed.

Results: Among the 1013 employees, there were 214 cases of COVID-19, nine required ward hospitalization, and no deaths occurred. In 26%, the probable source of contamination was occupational. From the first patient diagnosed with COVID-19 in 03/20/2020 till 10/21/2020, 523 deceased KT were performed, a 21% increase compared with 2019, with no confirmed donor-derived SARS-CoV-2 infection. Four patients were transplanted with a positive pretransplant SARS-CoV-2 test, but none of them developed the disease. Overall, of 11 875 KT followed in our center, 674 developed COVID-19. Among the hospitalized, 53% required mechanical ventilation, and 45% required hemodialysis. Their overall mortality rate was 27.5%.

Conclusion: This experience shows the challenges that transplant centers faced as the pandemic unfolded and illustrates the effectiveness of the sequential measures implemented to provide a safe environment for transplantation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tid.13600DOI Listing
March 2021

Antidiarrheal Effect of Vogel (Leguminosae) on Mice Involves Adrenergic Pathway Activation.

Evid Based Complement Alternat Med 2021 28;2021:1385606. Epub 2021 Feb 28.

Programa de Pós-graduação Em Produtos Naturais e Sintéticos Bioativos/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.

Several secondary metabolites have been isolated from (Leguminosae), mainly flavonoids. These compounds are known for many pharmacological actions, such as antispasmodic and antidiarrheal. Therefore, we evaluated the antidiarrheal effect of the ethanolic extract obtained from aerial parts (ZB-EtOH), as well as its underlying mechanisms. Castor-oil-induced diarrhea, fluid accumulation, and intestinal transit (normal and castor oil induced) were performed to assess the antidiarrheal, antisecretory, and antipropulsive activities of the extract. The involvement of opioid and adrenergic pathways was also investigated. ZB-EtOH inhibited, in a dose-dependent manner, both total defecation frequency and the number of watery stools. The extract showed no effect on fluid accumulation or normal intestinal transit. On the other hand, when the animals were pretreated with castor oil, the extract decreased the distance traveled by the marker in the small intestine. Investigation of the involvement of opioid and adrenergic systems showed that the pharmacological potency of the extract did not change in the presence of naloxone, but it was reduced in the presence of yohimbine. The data indicate that has an antidiarrheal effect due to inhibition of the intestinal motility through adrenergic pathway activation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/1385606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939745PMC
February 2021

Effects of reservoir cascades on diversity, distribution, and abundance of fish assemblages in three Neotropical basins.

Sci Total Environ 2021 Mar 5;778:146246. Epub 2021 Mar 5.

Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Departamento de Biologia, Centro de Ciências Biológicas, Universidade Estadual de Maringá, Paraná, Brazil; Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá (UEM), Paraná, Brazil.

River systems are characterized by the existence of longitudinal processes structuring fish assemblages. However, the construction of dams, many of them built in cascades, are disrupting these processes worldwide. Here, we analyzed the fish assemblages across reservoir cascades in three Brazilian river basins (Iguaçu, Paranapanema, and São Francisco) to identify whether there is a spatial convergent pattern and to infer the mechanisms structuring metacommunities in these Neotropical rivers. Linear models were used to assess the effect of reservoir cascades, and the associated morphological, spatial and environmental variables, on the species richness and diversity along them. We analyzed if reservoir cascades produce similar species distribution patterns using the elements of metacommunity structure framework and beta diversity and its components. Finally, super-organizing maps were used to find common trends in species abundances and the environmental, morphological, and spatial variables along cascades. The negative relationship between species richness and diversity and the position in the cascade indicated diversity declines along cascades. However, the resulting metacommunities varied in each river basin. They conformed a quasi-Gleasonian structure, a Clementsian structure, and a nested structure with stochastic species loss in the Iguaçu, Paranapanema, and São Francisco River basins, respectively. Generally, total beta-diversity (βsor) and species turnover (βsim) between pairs of reservoirs increased along reservoir cascades, especially at the downstream end, whereas nestedness (βsne) depicted distinct trends in each river basin. By contrast, there were general decreases in species abundances along cascades, especially downstream the fourth reservoir, with very few species benefiting from such situation. In general, species present in the downstream reservoirs were subsets of the species present in the upstream reservoirs (particularly in the São Francisco River Basin), while some had singular responses to the environmental gradient and appeared or disappeared at random. Although the cascade has an effect on fish assemblages, reservoir characteristics and operation also influence them. Our study highlights the impact of such structures and shows general patterns of fish assemblages that should help to mitigate the resulting ecological impacts and assist the process of infrastructure planning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.146246DOI Listing
March 2021

Biochemical and blood gas alterations in buffalo (Bubalus bubalis) whole blood stored in CPDA-1 and CPD/SAG-M bags.

J Vet Emerg Crit Care (San Antonio) 2021 Mar 12;31(2):269-273. Epub 2021 Mar 12.

Laboratory of Animal Health (LARSANA), Federal University of Western Pará (UFOPA), Santarém, Brazil.

Objective: The objective of this study was to evaluate the biochemical and blood gas alterations of whole blood of buffaloes that was stored in citrate-phosphate-dextrose with adenine (CPDA-1) and CPD/SAG-M blood bags for 42 days.

Design: Prospective study.

Interventions: Ten male buffaloes were used in this study. A total volume of 900 mL of blood was collected from each buffalo so that 450 mL was stored in CPDA-1 and 450 mL was stored in CPD/SAG-M bags at 2-6°C for 42 days. The stored blood was evaluated at 7 time points (D): D0 (immediately after blood collection) and 7 (D7), 14 (D14), 21 (D21), 28 (D28), 35 (D35), and 42 (D42) days after collection. Blood gas, biochemical, and microbiological parameters were monitored.

Measurements And Main Results: The overall blood pH decreased from 6.997 ± 0.05 at D0 to 6.784 ± 0.09 at D42, differing from baseline from D14 onward (P < 0.05). There were increases in partial pressure of oxygen (pO ), partial pressure of carbon dioxide (pCO ), lactate, and potassium (K) and decreases in the concentrations of sodium, bicarbonate, glucose, and pH (P < 0.05) during storage in both bags but no alterations in total protein concentration. Most of the variables were consistently similar between the 2 types of blood bags (P > 0.05) evaluated, with the exception of pCO , HCO cholesterol, and total protein, which had higher values in the CPDA-1 bag (P < 0.05). The K, pO , and lactate had the highest alterations during storage, with increases from baseline to D42 of 563%, 317%, and 169%, respectively.

Conclusion: In general, no significant changes of clinical importance were observed after storage of whole blood samples from buffaloes for 42 days in the 2 types of blood bags that are indicated for use with this species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/vec.13053DOI Listing
March 2021

The Emerging Role of Iron Acquisition in Biofilm-Associated Infections.

Trends Microbiol 2021 Mar 8. Epub 2021 Mar 8.

Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal. Electronic address:

A possible association between iron and biofilm formation has been explored for a long time. Here, we focus on major recent advances that shed light on the mechanisms behind this relationship and discuss how siderophore-mediated iron acquisition may impact the virulence of important nosocomial pathogens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tim.2021.02.009DOI Listing
March 2021

Swallowing in behavioral variant frontotemporal dementia.

Arq Neuropsiquiatr 2021 01;79(1):8-14

Universidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Neurologia e Neurocirurgia, São Paulo SP, Brazil.

Background: Swallowing and feeding problems may occur with the progression of behavioral variant frontotemporal dementia (bvFTD) and can impair the anticipatory and oral preparatory phases of swallowing.

Objective: To characterize swallowing problems and the feeding situation of patients with bvFTD and to correlate the swallowing problems with functionality, executive functions, cognitive and behavioral features.

Methods: Consecutive outpatients with bvFTD in mild, moderate and severe dementia stages were recruited along with their caregivers. Patients and caregivers were screened with the following scales: "Mini-Mental State Examination", "Severe Mini-Mental State Examination", "FTLD-modified Clinical Dementia Rating", "Neuropsychiatric Inventory", "Frontal Assessment Battery", "Index of Independence in Activities of Daily Living", "Swallowing Rating Scale" and "Assessment of Feeding and Swallowing Difficulties in Dementia".

Results: Overall, thirty patients with bvFTD were included along with their caregivers. Patients with bvFTD showed feeding and swallowing difficulties such as: messy to eat, passivity, coughing and choking, difficulty with some food consistencies and with specific food. Swallowing problems in bvFTD correlated with impaired functionality (p<0.05) and cognition (p<0.05), executive dysfunction (p<0.01) and behavioral features (p<0.01). Caregivers had great difficulty in managing the feeding situation during mealtime, with different characteristics in each dementia stage.

Conclusion: Patients with bvFTD had inappropriate speed eating, passivity, coughing and choking starting in the mild dementia stage, and these problems worsen in the severe stage. Such difficulties affected caregiver performance during mealtime. The correlations indicated that swallowing difficulties tend to follow cognitive and behavioral decline in patients with bvFTD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/0004-282X20200060DOI Listing
January 2021

Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation.

Bio Protoc 2020 Feb 20;10(4):e3534. Epub 2020 Feb 20.

Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of abdominal and inguinal adipose tissues, MSCs are selected by their adherence to the plastic tissue culture dish. Within 7 days, MSCs reach 70% confluence and are ready to be used in subsequent experiments. The protocols described here are easy to perform, cost-efficient, require minimal time, and yield a cell population rich in MSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21769/BioProtoc.3534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842647PMC
February 2020

Efficiency of lysine utilization by growing meat quail.

Poult Sci 2021 Apr 23;100(4):101012. Epub 2021 Jan 23.

Center of Agrarian and Environmental Sciences, Federal University of Maranhão, Campus Chapadinha, Chapadinha, Maranhão, Brazil.

The objective of present study was to estimate the efficiency of lysine utilization by meat quail of 21 to 35 d of age. A total of 500 meat quails were distributed in a completely randomized design in a 2 × 5 factorial arrangement, with 2 sexes (male and female) and 5 digestible lysine levels (0.714, 0.816, 0.918, 1.020, and 1.122%) and 5 replications of 10 birds each. The variables studied were feather-free body weight (FFBW), feed intake (FI), lysine intake (LysI), feather-free body protein deposition (FFBPD), feather-free body lysine deposition (FFBLysD), feather-free body fat deposition (FFBFatD), feather weight (FW), feather protein deposition, feather lysine deposition, and feather fat deposition. The FFBW, FFBPD, FFBLysD, and FFBFatD were regressed as a function of LysI for each sex to estimate the efficiency of lysine utilization in the feather-free body (ELysFFB), and the individual equations were compared. In addition, a multiple regression without intercept was also used to estimate the ELysFFB and in feathers (ELysF) individually. To compare the ELysFFB obtained by the different methods, the t-statistic was used. There was no effect on sex × lysine level interactions for any variable. The females showed higher FFBW (5.07%) and FFBFatD (26.23%) than males. All variables increased with the level of dietary lysine, with the exception of FI, FW, and the deposition of nutrients within them. The ELysFFB values obtained by simple linear regression and multiple linear regression were 48.0 and 44.6%, respectively. As there was no difference in the efficiencies estimated by the different methodologies, the best estimate of ELysFFB was 46.3%, that is, the average. The best estimate of ELysF was 18.1%, obtained by multiple linear regression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.psj.2021.01.034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921006PMC
April 2021

Fungal diversity present on rocks from a polar desert in continental Antarctica assessed using DNA metabarcoding.

Extremophiles 2021 Mar 2;25(2):193-202. Epub 2021 Mar 2.

Laboratório de Microbiologia Polar e Conexões Tropicais, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, PO Box 486, Belo Horizonte, MG, CEP 31270-901, Brazil.

We evaluated the fungal diversity associated with carbonate veins and two types of salt encrustation in rocks in a polar desert region of continental Antarctica using DNA a metabarcoding approach. We detected 262,268 reads grouped into 517 amplicon sequence variants (ASVs) assigned to the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Fourteen ASVs belonging to the genera Trichosporon, Mortierella, Penicillium, Aspergillus, Cladosporium, Coprinellus, Pleurotus and Pseudogymnoascus were assessed to be dominant taxa. The fungal communities of the three habitats sampled displayed high diversity indices when compared with other habitats of Antarctica, although differing in detail, with the highest diversity indices in the gypsum crust type 2. Only 48 of the 517 ASVs (9.28%) were detected in all three habitats, including dominant, intermediate and minor components. In contrast with previous studies of fungal communities living in the ultra-extreme conditions of continental Antarctica, application of metabarcoding revealed the DNA of a rich and complex resident fungal community. The ASVs detected included fungi with different ecological roles, with xerophilic, human- and animal-associated, phytopathogenic, saprotrophic, mutualistic, psychrotolerant and cosmopolitan taxa. This sequence diversity may be the result of deposition of fungal propagules over time driven by air currents, precipitation or human activities in the region.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00792-021-01221-4DOI Listing
March 2021

Plastid role in phytomelanin synthesis in Piptocarpha axillaris (Less.) Baker stems (Asteraceae, Vernonieae).

Protoplasma 2021 Mar 2. Epub 2021 Mar 2.

Programa de Pós-Graduação em Biologia de Fungos, Algas e Plantas (PPGFAP), Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, 88040-900, Brazil.

Phytomelanin is a brown to black pigment found in plant tissues, mainly in Asparagales and Asteraceae species. However, few studies deal with the processes of its synthesis, and there are still many questions to be answered regarding the organelles involved in this process and their functions, especially in vegetative organs. In a previous study with stems and leaves of 77 Vernonieae (Asteraceae) species, phytomelanin was demonstrated to always be associated with sclereids, which suggests the involvement of these cells in the pigment synthesis. Thus, we selected another species of tribe Vernonieae, Piptocarpha axillaris (Less.) Baker, which produces abundant phytomelanin secretion in stem tissues, to investigate which cells and organelles are involved in the synthesis and release of this pigment, as well as its distribution in the tissues. To achieve this goal, stems in different developmental phases were analyzed under light and transmission electron microscopy. Anatomical analysis showed that the polymerization of phytomelanin in P. axillaris starts at the second stem node, in the pith region, and occurs simultaneously with sclereid differentiation. The plastids of cells that will differentiate into sclereids actively participate in the phenolic material synthesis, following the "tannosome" and the "pearl necklace" models, giving rise to the main precursor of phytomelanin, which is then polymerized in the intercellular spaces during the sclerification process of sclereids. In stems with an established secondary structure, the pigment can be observed more frequently in the cortex, pericycle, primary phloem, secondary phloem, and pith.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00709-021-01615-3DOI Listing
March 2021

The isolated or combined effects of dynamic controlled atmosphere (DCA) and 1-MCP on the chemical composition of cuticular wax and metabolism of 'Maxi Gala' apples after long-term storage.

Food Res Int 2021 Feb 10;140:109900. Epub 2020 Nov 10.

Department of Technology and Food Science, Federal University of Santa Maria (UFSM), 97015-900 Santa Maria, RS, Brazil. Electronic address:

Apples have a continuous hydrophobic layer that covers the surface of the fruit, which is called the cuticle. The effects of 1-methylcyclopropene (1-MCP) on the cuticular wax layer of apples were reported after cold storage, although the interaction between 1-MCP and dynamic controlled atmosphere (DCA) is not yet known. Therefore, this study aimed to analyze the effects of 1-MCP on the wax composition and metabolism of 'Maxi Gala' apples after storage in a controlled atmosphere (CA) and dynamic controlled atmosphere based on chlorophyll fluorescence (DCA-CF) and respiratory quotient (DCA-RQ; RQ = 1.3 and 1.5). The 1-MCP treatment effectively decreased ethylene production for CA and DCA-CF treatments, while in DCA-RQ treatments produced no effect. The average extracted cuticular wax content of 'Maxi Gala' apples was 16.65 g m and no differences in storage conditions or 1-MCP application were observed. Alkanes, alcohols, fatty acids, aldehydes, and terpenoids were identified in the chemical composition of the cuticular waxes, being alkanes and fatty acids the predominant ones. Moreover, 1-MCP decreased fatty acid and 10-nonacosanol concentrations in the fruit. Fruit with the 1-MCP application and stored in DCA had lower α-farnesene concentrations. The wax compositions of the DCA-stored apples with and without 1-MCP were similar. However, 1-MCP treatment resulted in a greater mass loss in fruit stored in DCA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2020.109900DOI Listing
February 2021

Fabrication of Quasi-2D Shape-Tailored Microparticles using Wettability Contrast-Based Platforms.

Adv Mater 2021 Apr 28;33(14):e2007695. Epub 2021 Feb 28.

Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal.

The ability to fabricate materials with ultrathin architectures enables the breakthrough of low-dimensional structures with high surface area that showcase distinctive properties from their bulk counterparts. They are exploited in a wide range of fields, including energy harvesting, catalysis, and biomedicine. Despite such versatility, the fine tuning of the lateral dimensions and geometry of these structures remains challenging. Prepatterned platforms gain significant attention as enabling technologies to process materials with highly controlled shapes and dimensions. Herein, different nanometer-thick particles of various lateral sizes and geometries (e.g., squares, circles, triangles, hexagons) are processed with high precision and definition, taking advantage of the wettability contrast of oleophilic-oleophobic patterned surfaces. Quasi-2D polymeric microparticles with high shape- and size-fidelity can be retrieved as freestanding objects in a single step. These structures show cell-mediated pliability, and their integration in gravity-enforced human adipose-derived stem cell spheroids leads to an enhanced metabolic activity and a modulated secretion of proangiogenic factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202007695DOI Listing
April 2021

Dementia in Latin America: Paving the way toward a regional action plan.

Authors:
Mario Alfredo Parra Sandra Baez Lucas Sedeño Cecilia Gonzalez Campo Hernando Santamaría-García Ivan Aprahamian Paulo Hf Bertolucci Julian Bustin Maria Aparecida Camargos Bicalho Carlos Cano-Gutierrez Paulo Caramelli Marcia L F Chaves Patricia Cogram Bárbara Costa Beber Felipe A Court Leonardo Cruz de Souza Nilton Custodio Andres Damian Myriam de la Cruz Roberta Diehl Rodriguez Sonia Maria Dozzi Brucki Lais Fajersztajn Gonzalo A Farías Fernanda G De Felice Raffaele Ferrari Fabricio Ferreira de Oliveira Sergio T Ferreira Ceres Ferretti Marcio Luiz Figueredo Balthazar Norberto Anizio Ferreira Frota Patricio Fuentes Adolfo M García Patricia J Garcia Fábio Henrique de Gobbi Porto Lissette Duque Peñailillo Henry Willy Engler Irene Maier Ignacio F Mata Christian Gonzalez-Billault Oscar L Lopez Laura Morelli Ricardo Nitrini Yakeel T Quiroz Alejandra Guerrero Barragan David Huepe Fabricio Joao Pio Claudia Kimie Suemoto Renata Kochhann Silvia Kochen Fiona Kumfor Serggio Lanata Bruce Miller Leticia Lessa Mansur Mirna Lie Hosogi Patricia Lillo Jorge Llibre Guerra David Lira Francisco Lopera Adelina Comas José Alberto Avila-Funes Ana Luisa Sosa Claudia Ramos Elisa de Paula França Resende Heather M Snyder Ioannis Tarnanas Jenifer Yokoyama Juan Llibre Juan Felipe Cardona Kate Possin Kenneth S Kosik Rosa Montesinos Sebastian Moguilner Patricia Cristina Lourdes Solis Renata Eloah de Lucena Ferretti-Rebustini Jeronimo Martin Ramirez Diana Matallana Lingani Mbakile-Mahlanza Alyne Mendonça Marques Ton Ronnielly Melo Tavares Eliane C Miotto Graciela Muniz-Terrera Luis Arnoldo Muñoz-Nevárez David Orozco Maira Okada de Oliveira Olivier Piguet Maritza Pintado Caipa Stefanie Danielle Piña Escudero Lucas Porcello Schilling André Luiz Rodrigues Palmeira Mônica Sanches Yassuda Jose Manuel Santacruz-Escudero Rodrigo Bernardo Serafim Jerusa Smid Andrea Slachevsky Cecilia Serrano Marcio Soto-Añari Leonel Tadao Takada Lea Tenenholz Grinberg Antonio Lucio Teixeira Maira Tonidandel Barbosa Dominic Trépel Agustin Ibanez

Alzheimers Dement 2021 02 20;17(2):295-313. Epub 2020 Nov 20.

Cognitive Neuroscience Center (CNC) Buenos Aires, Argentina; Universidad Autonoma del Caribe, Barranquilla, Colombia; Global Brain Health Institute (GBHI), US, Universidad de San Andres, CONICET, Universidad Autonoma del Caribe, Universidad Adolfo Ibanez, UCSF, USA.

Across Latin American and Caribbean countries (LACs), the fight against dementia faces pressing challenges, such as heterogeneity, diversity, political instability, and socioeconomic disparities. These can be addressed more effectively in a collaborative setting that fosters open exchange of knowledge. In this work, the Latin American and Caribbean Consortium on Dementia (LAC-CD) proposes an agenda for integration to deliver a Knowledge to Action Framework (KtAF). First, we summarize evidence-based strategies (epidemiology, genetics, biomarkers, clinical trials, nonpharmacological interventions, networking, and translational research) and align them to current global strategies to translate regional knowledge into transformative actions. Then we characterize key sources of complexity (genetic isolates, admixture in populations, environmental factors, and barriers to effective interventions), map them to the above challenges, and provide the basic mosaics of knowledge toward a KtAF. Finally, we describe strategies supporting the knowledge creation stage that underpins the translational impact of KtAF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/alz.12202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984223PMC
February 2021

Salivary Protease Inhibitors as Potential Anti-Tick Vaccines.

Front Immunol 2020 4;11:611104. Epub 2021 Feb 4.

Laboratory of Physiology of Hematophagous Insects, Department of Parasitology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

is the main tick associated with human bites in Brazil and the main vector of , the causative agent of the most severe form of Brazilian spotted fever. Molecules produced in the salivary glands are directly related to feeding success and vector competence. In the present study, we identified sequences of salivary proteins that may be involved in hematophagy and selected three proteins that underwent functional characterization and evaluation as vaccine antigens. Among the three proteins selected, one contained a Kunitz_bovine pancreatic trypsin inhibitor domain (named AsKunitz) and the other two belonged to the 8.9 kDa and basic tail families of tick salivary proteins (named As8.9kDa and AsBasicTail). Expression of the messenger RNA (mRNA) encoding all three proteins was detected in the larvae, nymphs, and females at basal levels in unfed ticks and the expression levels increased after the start of feeding. Recombinant proteins rAs8.9kDa and rAsBasicTail inhibited the enzymatic activity of factor Xa, thrombin, and trypsin, whereas rAsKunitz inhibited only thrombin activity. All three recombinant proteins inhibited the hemolysis of both the classical and alternative pathways; this is the first description of tick members of the Kunitz and 8.9kDa families being inhibitors of the classical complement pathway. Mice immunization with recombinant proteins caused efficacies against A. females from 59.4% with rAsBasicTail immunization to more than 85% by immunization with rAsKunitz and rAs8.9kDa. The mortality of nymphs fed on immunized mice reached 70-100%. Therefore, all three proteins are potential antigens with the possibility of becoming a new tool in the control of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.611104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901972PMC
February 2021

Can sucrose-substitutes increase the antagonistic activity against foodborne pathogens, and improve the technological and functional properties of sheep milk kefir?

Food Chem 2021 Jul 16;351:129290. Epub 2021 Feb 16.

Federal Fluminense University, Faculty of Veterinary, 24230-340 Niterói, RJ, Brazil. Electronic address:

The effect of different types of sugar (sucrose, demerara, brown, fructose, coconut sugar, and honey) on sheep milk kefir was evaluated. Microbial counts (Lactobacillus, Lactococcus, Leuconostoc, yeast), antagonistic activity against foodborne pathogens, microstructure (scanning electron microscopy), and antiproliferative activity of cancer cells were evaluated. Furthermore, the antioxidant activity (DPPH), inhibitory activity of angiotensin-converting enzyme (ACE), α-amylase, and α-glucosidase, lactose content, lactic and acetic acids and ethanol, fatty acid profile and volatile organic compounds were determined. The addition of sugars increased the Lactobacillus population (up to 2.24 log CFU/mL), metabolites concentration, antagonistic activity against pathogens, antioxidant activity (11.1 to 24.1%), ACE inhibitory activity (27.5 to 37.6%), α-amylase inhibition (18 to 37.4%), and anti-proliferative activity. Furthermore, it improved the fatty acid profile and volatile compounds. The results suggest that the replacement of sucrose with different types of sugar constitute an interesting option to be used in sheep milk kefir formulations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.129290DOI Listing
July 2021

Technological Advancements in Monoclonal Antibodies.

ScientificWorldJournal 2021 10;2021:6663708. Epub 2021 Feb 10.

University Center SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.

Biopharmaceuticals are innovative solutions that have revolutionized the treatment of important chronic diseases and malignancies. The approval of biosimilar products has become a complex and balanced process, and there are versions of drugs with established biosimilarity that can offer a more accessible treatment option to patients. The objective of this work was to identify the advancement of these technologies by means of patent and article analysis based on technological and scientific prospection. In patent document recovery, Derwent Innovation Index (DWPI) and PatentInspiration databases were used. The research was based on the search of the selected terms in the title, summary, and claims of the documents through a search strategy containing IPC code and keywords. In articles recovery, the Web of Science tool was used in the search of scientific publications dated from the last 5 years. The search resulted in a total of 2295 individual patent documents and 467 families using DWPI database, 769 individual patents and 205 families using PatentInspiration, and 2602 articles using Web of Science database. Additionally, this work describes the number of organizations that contribute to this area, where they are, how much development they have undergone, and the inventors/authors involved. Based on the number of publications registered, there is an important prominence for scientific research in mAbs. In terms of innovation, it is expected that several therapeutic drugs are already under regulatory review, which will allow drugs to be approved over the next few years and will thus generate a continuous flow of new products based on immunotherapies, mAbs, and biosimilar drugs. These drugs have become essential weapons for the treatment of significant diseases, and the increasing trend in the number of related scientific and technological publications contributes to making these therapies available to the greatest number of people.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6663708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892242PMC
February 2021