Publications by authors named "Fábio Rogério Brosci Garcia"

2 Publications

  • Page 1 of 1

A new experimental model to study preneoplastic lesions in achalasia of the esophagus.

Acta Cir Bras 2005 Nov-Dec;20(6):418-21. Epub 2005 Nov 8.

FMRP, University of São Paulo, Brazil.

Purpose: Develop an experimental model to study esophageal preneoplastic lesions induced by diethylnitrosamine in rats with achalasia.

Methods: Male Wistar rats were divided into four groups: control--C (n=8); rats with megaesophagus--B (n=8); rats treated with DEN--D (n=15) and rats with megaesophagus plus DEN--BD (n=15). Megaesophagus can be experimentally obtained in rats by topical application of benzalkonium chloride. The morphology and PCNA labeling index of the epithelium were evaluated.

Results: The morphometric analysis showed an increase in epithelial thickness in the animals of group BD (2166+/-1012 mm2) when compared to the other groups (C = 878+/-278 mm2; B = 1746+/-144 mm2 and D = 1691+/-697 mm2), mainly due to basal layer hyperplasia, besides an increase in the keratin of the superficial layer. The PCNA labeling index in the basal layer was significantly higher in the group BD (0.695+/-0.111) when compared to the other groups (C = 0.490+/-0.132; B = 0.512+/-0.215 and D = 0.477+/-0.198).

Conclusions: Our data confirm in an experimental model the previous observation in humans of increased epithelial cell proliferation during the esophageal carcinogenic process in achalasia and may be useful to further studies on the mechanisms of the esophageal carcinogenesis and the the design of follow-up endoscopic studies for patients with achalasia.
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July 2006

A retrospective study of histopathological findings in 894 cases of megacolon: what is the relationship between megacolon and colonic cancer?

Rev Inst Med Trop Sao Paulo 2003 Mar-Apr;45(2):91-3. Epub 2003 May 14.

Departament of Pathology, FMRP, USP, Ribeirão Preto, SP, Brazil.

Patients with megaesophagus (ME) have increased prevalence of cancer of the esophagus. In contrast, a higher incidence of colorectal cancer is not observed in patients with megacolon (MC). MC is very common in some regions of Brazil, where it is mainly associated with Chagas disease. We reviewed the pathology records of surgical specimens of all patients submitted for surgical resection of MC in the Hospital das Clínicas of the Faculty of Medicine of Ribeirão Preto (HC-FMRP), from the University of São Paulo. We found that 894 patients were operated from 1952 until 2001 for MC resection. Mucosal ulcers, hyperplasia and chronic inflammation were frequently found, while polyps were uncommon. No patients with MC presented any type of colonic neoplasm. This observation reinforces the hypothesis that MC has a negative association with cancer of the colon. This seems to contradict the traditional concept of carcinogenesis in the colon, since patients with MC presents important chronic constipation that is thought to cause an increase in risk for colon cancer. MC is also associated with other risk factors for cancer of colon, such as hyperplasia, mucosal ulcers and chronic inflammation. In ME these factors lead to a remarkable increase in cancer risk. The study of mucosal cell proliferation in MC may provide new insights and useful information about the role of constipation in colonic carcinogenesis.
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October 2003