Publications by authors named "Fábio Alexandre Chinalia"

15 Publications

  • Page 1 of 1

Screening and testing potential inhibitors of sulphide gas production by sulphate-reducing bacteria.

J Mol Model 2021 May 27;27(6):189. Epub 2021 May 27.

Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s/n - Vale do Canela, Salvador, BA, 40.231-300, Brazil.

Sulphate-reducing bacteria are commonly associated with biological causes of oil well souring. Biosulphetogenesis can directly affect oil quality and storage due to the accumulation of sulphides. In addition, these microorganisms can create bio-incrustation that can clog pipes. Sulphite reductase (SIR) is the enzyme responsible for converting ion sulphite into sulphide and several substances may interfere or control such activity. This interference can hinder growth of the sulphate-reducing bacteria and, consequently, it reduces sulphide accumulation in situ. This work focuses on molecular modelling techniques along with in vitro experiments in order to investigate the potential of two essential oils and one vegetable oil as main inhibitors of sulphite reductase activity. Docking simulation identified several substances present in Rosmarinus officinalis, Tea tree and Neem extractable oils as potential inhibitors of SIR. Substances present in Neem vegetable oil are the most potent inhibitors, followed by Rosmarinus officinalis and Tea tree essential oils. The Neem oil mixture showed a superior effectiveness in intracellular SIR inhibitory effects.
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http://dx.doi.org/10.1007/s00894-021-04801-5DOI Listing
May 2021

Essential and Potentially Toxic Elements from Brazilian Geopropolis Produced by the Stingless Bee Melipona quadrifasciata anthidioides Using ICP OES.

Biol Trace Elem Res 2021 Sep 23;199(9):3527-3539. Epub 2020 Oct 23.

Institute of Health Sciences, Universidade Federal da Bahia, Salvador, BA, 40110-100, Brazil.

Melipona quadrifasciata anthidioides is a species of stingless bee popularly known in Brazil as "mandaçaia". Products derived from bees for food and therapeutic uses, have stimulated the evaluation of the chemical composition of geopropolis. Concentrations of 24 essential and potentially toxic elements were determined in geopropolis samples, using a sequential optical emission spectrometer with inductively coupled plasma (ICP OES) after microwave-assisted acid digestion. Principal component analysis (PCA) and Hierarchical cluster analysis (HCA) were used to carry out an exploratory analysis of the samples. The following elements were quantified (in mg Kg): Al (320,414.40-36,911.1), As (
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http://dx.doi.org/10.1007/s12011-020-02455-7DOI Listing
September 2021

Bacterial xanthan and ramnolipid simultaneous production using industrial oil produced water.

Environ Technol 2020 Sep 14:1-8. Epub 2020 Sep 14.

Biointeraction Sciences Department, Federal University of Bahia, Salvador, Brazil.

The present work aimed to give an economical destiny to the produced water, a residue generated by the oil and gas industry by means of producing bioactives such as xanthan gum and ramnolipid. These compounds are often used in combination during enhanced oil recovery strategies. On the other hand, reports on co-culture of bacterial strains that are responsible for their production are rare. This research shows a factorial design method associated with surface response analysis to optimize carbon sources, sucrose and crude glycerin, and fermentation agents for culturing and using the described conditions. After the critical point validation resulting in xanthan and ramnolipid production of 8.69 and 4.80 g L, quality tests showed an apparent viscosity of 1006 cP with an emulsifying activity abouve 50% for 94 h.
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http://dx.doi.org/10.1080/09593330.2020.1812729DOI Listing
September 2020

Sunscreen protection factor enhancement through supplementation with Rambutan (Nephelium lappaceum L) ethanolic extract.

J Photochem Photobiol B 2020 Apr 21;205:111837. Epub 2020 Feb 21.

Postgraduate Program in Biotechnology, Health Sciences Institute, Federal University of Bahia, Principal Miguel Calmon Avenue, Canela Valley, Zip code 40110-100 Salvador, Bahia, Brazil. Electronic address:

The use of plant bioactives in cosmetic formulations are increasing due to the search for new sustainable sources of raw materials, greater concern for safety and possible social impacts caused by the incorrect use of natural resources. Plant extracts have been the subject of several studies in several industrial areas. However, the potential use of tropical fruits extracts in cosmetic formulations remains largely unexplored. Considering the impact on public health and the possibility of using wastes from fruit processing, the aim of this work is to identify, evaluate and develop a sunscreen based on rambutan peel extracts (Nephelium lappaceum L) as a natural additive for enhancing the final product sun protection factor (SPF). The phytochemical screening revealed the presence of tannins and flavonoids and the absence of coumarins. Even presenting a low sunscreen factor when used alone (SPF value 0.4), rambutan extract at 1.00% FTP concentration improved the photoprotective result (11.2) of the formulation containing 7.5% of ethylhexyl metoxycinnamate (EHMC) by 134%. The addition of rambutan extract in the formulation shows the potential to reduce the use of synthetic photoprotectors by about 64% of the total synthetic organic filters used to achieve the SPF value of 26.3. In addition, the sunscreen formulation supplemented with rambutan extract containing 1.00% RTP shows the potential to minimize the risk of synthetic agent toxicity and a 45% reduction in the cost of sunscreen production.
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http://dx.doi.org/10.1016/j.jphotobiol.2020.111837DOI Listing
April 2020

Guava-fruit extract can improve the UV-protection efficiency of synthetic filters in sun cream formulations.

J Photochem Photobiol B 2019 Dec 19;201:111639. Epub 2019 Oct 19.

Postgraduate Program in Biotechnology, Health Sciences Institute, Federal University of Bahia, Principal Miguel Calmon Avenue, Canela Valley, CEP 40110-100 Salvador, Bahia, Brazil. Electronic address:

Increasing concerns about health safety, social impacts and fair trade have intensified the industrial interest for using natural products in commercial cosmetic formulations. Several studies are currently focusing on plant extracts, but tropical fruits, such as guava, remain yet unexplored and, consequently, underutilized. This research aims to evaluate the potential for using guava-fruit extract as a photoprotective additive agent for sun cream formulations. The phytochemical screening revealed the presence of flavonoids and tannins and the absence of coumarins. Although the Psidium guajava extract showed a low sun protection factor (SPF) value (1.0), it improved in about 134% the photoprotective result (8.1) of 7.5% 2-ethyl-hexyl methoxycinnamate formulation. Therefore, guava-fruit extract supplementation in the formulation shows the potential to reduce the use of synthetic photoprotectors in about 78.9% from the total synthetic organic filters used to achieve the SPF value of 18. Thus, sun cream supplemented with guava-fruit extract show the potential for minimizing the risk of synthetic agent toxicity, and a 65.8% reduction in the cost of the sunscreen production.
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http://dx.doi.org/10.1016/j.jphotobiol.2019.111639DOI Listing
December 2019

Glucose and Nitrogen Amendments Can Mitigate Wastewater-Borne Bacteria Competition Effect Against Algal Growth in Wastewater-Based Systems.

J Phycol 2019 10 28;55(5):1050-1058. Epub 2019 Aug 28.

Departmento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. Reitor Miguel Calmon s/n Vale do Canela, Salvador, Bahia, 40110-100, Brazil.

The reuse of wastewater is important for reducing costs involved with algal lipid production. However, nutrient limitations, wastewater-borne microbes, and mixotrophic growth can significantly affect biomass yields and lipid/biomass ratios. This research compared the growth performances of both Chlorella vulgaris and Pseudokirchneriella subcapitata on domestic wastewater effluent. The experiments were conducted in the presence and absence of wastewater-borne bacteria, while additionally assessing the impact of distinct nitrate and glucose supplementations. When compared to the sterilized controls, the presence of wastewater-borne bacteria in the effluent reduced C. vulgaris and P. subcapitata total biomass production by 37% and 46%, respectively. In the corresponding treatments supplemented with glucose and nitrate, total biomass production increased by 12% and 61%, respectively. The highest biomass production of 1.11 and 0.72 g · L was, however, observed in the sterilized treatments with both glucose and nitrate supplementations for C. vulgaris and P. subcapitata, respectively. Lipid to biomass ratios were, on average, threefold higher when only nitrate was introduced in the sterilized treatments for both species (0.4 and 0.5, respectively). Therefore, the combination of nitrate and glucose supplementation is shown to be an important strategy for enhancing algal lipid and biomass production when those algae are grown in the presence of wastewater-borne bacteria. On the other hand, in the absence of wastewater-borne bacteria, only nitrate supplementation can significantly improve lipid/biomass ratios.
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http://dx.doi.org/10.1111/jpy.12902DOI Listing
October 2019

Photobiological effect of Laser or LED light in a thermophilic microbial consortium.

J Photochem Photobiol B 2018 Apr 8;181:115-121. Epub 2018 Mar 8.

Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA 40110-150, Brazil; National Institute Basic Optics and Applied to Life Science, 400, Trabalhador São-Carlense Ave, Parque Arnold Schimidt, São Carlos, SP 13566-590, Brazil; Biomedical Engineering PhD Program, Brazil University, 235, Carolina Fonseca St, Itaquera, São Paulo, SP 08230-030, Brazil. Electronic address:

Cellulose has a highly diversified architecture and its enzymatic complexes are studied for achieving an efficient conversion and a high level of efficiency in the deconstruction of cellulolytic biomass into sugars. The aim of this investigation was to evaluate the effect of Laser or LED light in the cellulolytic activity (CMCase) and on the proliferation of the thermophilic microbial consortium used on the degradation process of a lignocellulosic biomass of green coconut shell. The irradiation protocol consisted of six Laser irradiations (λ660 ηm, 40 mW, 270 s, 13 J/cm) or LED (λ632 ± 2 ηm, 145 mW, 44 s, 13 J/cm) with 12- h time intervals in nutrient deprivation conditions. After irradiation, the consortium was inoculated into a lignocellulosic biomass (coconut fibers). Non- irradiated consortium was also inoculated and acted as control. Cell proliferation and endoglucanase activity were quantified during the experimental time. Experiments were carried out in triplicate. The results showed an increase of 250 % of thermo-cellulolytic microorganisms for the LED group and 200% for the Laser group when compared to the control. The enzymatic index (red Congo method), showed a statistically significant difference in the process of degradation of the lignocellulosic biomass between the Laser and LED groups compared to the control group [p < 0.0029; p < 0.029, respectively] 48-hs after the inoculation of the microorganisms. At the end of 72-h, this significant difference was maintained for both irradiated groups (p < 0.0212). Based upon the protocol used on the present study, it is possible to concluded that LED light enhanced cell proliferation of the thermophilic microbial consortium while the Laser light increase the enzymatic index of the lignocellulosic biomass of green coconut shell.
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http://dx.doi.org/10.1016/j.jphotobiol.2018.03.006DOI Listing
April 2018

The potential impact of using a surfactant and an alcoholic co-surfactant on SRB activity during EOR.

Environ Technol 2019 Jul 15;40(16):2100-2106. Epub 2018 Feb 15.

a Departamento de Biointeração, Instituto de Ciências da Saúde (ICS), Universidade Federal da Bahia , Salvador , Brazil.

Surfactants and co-surfactants play an important role in enhanced oil recovery for they improve petroleum solubility and reduce interfacial tensions between oil, water and the rock formation. Ethanol is receiving renewed attention as potential co-surfactant because of the negative results obtained with the use of salts and alkaline substances. Sulphate-reducing bacteria (SRB) can use surfactants and co-surfactants as carbon sources and, consequently, this can increase the biological accumulation of sulphide (souring). The aim of this research is to correlate SRB activity with different concentrations of co-surfactant (ethanol) as an attempt to quantifying in which concentration such compound can potentially increase or inhibit souring. The results show that the combination of surfactant (lauryl glucoside) and co-surfactant (ethanol) can increase SRB activity to about 2.3-fold. The highest sulphate consumption rate of 591 μg l h was observed in experiments with 0.03% and 1.5% (v/v) of surfactant and ethanol, respectively. The experiments indicated that SRB activity is only controlled by ethanol concentrations above 6.5% (v/v). Ethanol can potentially decrease costs with the use of biocides and significantly increase oil recovery ratios. Tests with the model were not comparable with the results obtained with the SRB consortium.
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http://dx.doi.org/10.1080/09593330.2018.1437780DOI Listing
July 2019

Characterization of isolates of equine infectious anemia virus in Brazil.

Arch Virol 2017 Mar 28;162(3):873-877. Epub 2016 Nov 28.

Laboratory of Virology, Institute of Health Science, Universidade Federal da Bahia (UFBA), Salvador, Brazil.

Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
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http://dx.doi.org/10.1007/s00705-016-3172-5DOI Listing
March 2017

Human bocavirus in acute gastroenteritis in children in Brazil.

J Med Virol 2016 Jan 25;88(1):166-70. Epub 2015 Jun 25.

Laboratory of Virology-Health Science Institute, Federal University of Bahia, Salvador, Bahia, Brazil.

Epidemiological surveillance for Human Bocavirus (HBoV) was conducted on 105 fecal specimens from children with acute gastroenteritis in Bahia, Brazil. Among of a total 105 stool samples, 44 samples were positive for HBoV as detected by nested-PCR. Of the 44 positive samples, co-infections with other enteric viruses (Norovirus, Adenovirus, and Rotavirus) were found in 12 pediatric patients. Mixed infections among HBoV with Norovirus were frequently observed in this population. The phylogenetic analysis identified the presence of HBoV-1, and HBoV 2A species. This study shows that HBoV is another viral pathogen in the etiology of acute gastroenteritis in children in Bahia, Brazil.
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http://dx.doi.org/10.1002/jmv.24293DOI Listing
January 2016

Growth of Chlorella vulgaris on sugarcane vinasse: the effect of anaerobic digestion pretreatment.

Appl Biochem Biotechnol 2013 Dec 7;171(8):1933-43. Epub 2013 Sep 7.

Laboratório de Biologia Marinha Instituto de Biologia (IB), Universidade Federal da Bahia/Brasil, Salvador, Brazil.

Microalgae farming has been identified as the most eco-sustainable solution for producing biodiesel. However, the operation of full-scale plants is still limited by costs and the utilization of industrial and/or domestic wastes can significantly improve economic profits. Several waste effluents are valuable sources of nutrients for the cultivation of microalgae. Ethanol production from sugarcane, for instance, generates significant amounts of organically rich effluent, the vinasse. After anaerobic digestion treatment, nutrient remaining in such an effluent can be used to grow microalgae. This research aimed to testing the potential of the anaerobic treated vinasse as an alternative source of nutrients for culturing microalgae with the goal of supplying the biodiesel industrial chain with algal biomass and oil. The anaerobic process treating vinasse reached a steady state at about 17 batch cycles of 24 h producing about 0.116 m(3)CH4 kgCODvinasse (-1). The highest productivity of Chlorella vulgaris biomass (70 mg l(-1) day(-1)) was observed when using medium prepared with the anaerobic digester effluent. Lipid productivity varied from 0.5 to 17 mg l(-1) day(-1). Thus, the results show that it is possible to integrate the culturing of microalgae with the sugarcane industry by means of anaerobic digestion of the vinasse. There is also the advantageous possibility of using by-products of the anaerobic digestion such as methane and CO2 for sustaining the system with energy and carbon source, respectively.
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http://dx.doi.org/10.1007/s12010-013-0481-yDOI Listing
December 2013

Comparing the use of different domestic wastewaters for coupling microalgal production and nutrient removal.

Bioresour Technol 2013 Mar 4;131:429-36. Epub 2013 Jan 4.

Environmental Technologies Department, University of Cádiz, Centro Andaluz de Ciencia y Tecnología Marinas (CACYTMAR), Spain.

The streams from municipal wastewater treatment plants (WWTP) have been considered a valuable medium for mass cultivation of algal biomass. The aim of this work is to test and compare the performance of Chlorella vulgaris on several streams from five stages, from two different WWTP. The results showed biomass yields ranging from 39 to 195mg dry-weightl(-1)days(-1). The best performance as biomass production was obtained with the centrate (effluent from drying the anaerobic sludge). After testing a wide range of N/P ratios with centrate, the highest productivity and growth rates were obtained with the original N/P ratio (2.0) of this stream. The highest removal rates were of 9.8 (N) and 3.0 (P) mgl(-1)days(-1), in the centrate. Finally, this research also suggests that microalgal production seems to be a promising process when coupled to wastewater treatment.
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http://dx.doi.org/10.1016/j.biortech.2012.12.152DOI Listing
March 2013

Unusual bacterial populations observed in a full-scale municipal sludge digester affected by intermittent seawater inputs.

J Ind Microbiol Biotechnol 2009 May 27;36(5):769-73. Epub 2009 Mar 27.

Department of Microbiology and Environmental Change Institute, Microbial Ecology Laboratory, National University of Ireland, Galway, Ireland.

This study investigated the bacterial community of a full-scale anaerobic digester, which suffers from intermittent seawater contaminations, using 16S rRNA gene clone analysis over different seasons. Bacteroidetes, Proteobacteria, and unclassifiable bacteria were the three major bacterial groups within the clone library (a total of 290 clones). A significant portion of the total clones (29.3%) was not affiliated to any previously reported phylum, and 55.3% of the unclassifiable clones (16.9% of the total clones) showed potential relations to the species of Thermotogae, rarely present under normal mesophilic anaerobic conditions. These results suggested that the novel populations may have the potential to play an important role in anaerobic processes, particularly under abnormal environmental conditions. Additionally, statistical analysis supported that seasonal variations in influent characteristics, and potential competitions among different populations, may be related to the unusual bacterial diversity and community dynamics observed over the study period.
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http://dx.doi.org/10.1007/s10295-009-0557-4DOI Listing
May 2009

Removal of ammonium via simultaneous nitrification-denitrification nitrite-shortcut in a single packed-bed batch reactor.

Bioresour Technol 2009 Feb 14;100(3):1100-7. Epub 2008 Sep 14.

Departmento of Hidráulica e Saneamento, Escola de Engenharia e São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.

A polyurethane packed-bed-biofilm sequential batch reactor was fed with synthetic substrate simulating the composition of UASB reactor effluents. Two distinct ammonia nitrogen concentrations (125 and 250 mg l(-1)) were supplied during two sequential long-term experiments of 160 days each (320 total). Cycles of 24h under intermittent aeration for periods of 1h were applied, and ethanol was added as a carbon source at the beginning of each anoxic period. Nitrite was the main oxidized nitrogen compound which accumulated only during the aerated phases of the batch cycle. A consistent decrease of nitrite concentration started always immediately after the interruption of oxygen supply and addition of the electron donor. Removal to below detection limits of all nitrogen soluble forms was always observed at the end of the 24h cycles for both initial concentrations. Polyurethane packed-bed matrices and ethanol amendments conferred high process stability. Microbial investigation by cloning suggested that nitrification was carried out by Nitrosomonas-like species whereas denitrification was mediated by unclassified species commonly observed in denitrifying environments. The packed-bed batch bioreactor favored the simultaneous colonization of distinct microbial groups within the immobilized microbial biomass. The biofilm was capable of actively oxidizing ammonium and denitrification at high ratios in intermittent intervals within 24h cycles.
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http://dx.doi.org/10.1016/j.biortech.2008.08.003DOI Listing
February 2009

Performance of a reactor containing denitrifying immobilized biomass in removing ethanol and aromatic hydrocarbons (BTEX) in a short operating period.

J Hazard Mater 2007 Jan 15;139(2):301-9. Epub 2006 Jun 15.

Laboratory of Biological Processes, Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo (USP), Av. Trabalhador São-carlense 400, São Carlos 13566-590, SP, Brazil.

A horizontal-flow anaerobic immobilized biomass reactor (HAIB) containing denitrifying biomass was evaluated with respect to its ability to remove, separately and in a short operating period (30 days), organic matter, nitrate, and the hydrocarbons benzene (41.4 mg L-1), toluene (27.8 mg L-1), ethylbenzene (31.1 mg L-1), o-xylene (28.5 mg L-1), m-xylene (28.4 mg L-1) and p-xylene (32.1 mg L-1). The purified culture, which was grown in the presence of the specific hydrocarbon, was used as the source of cells to be immobilized in the polyurethane foam. After 30 days of operation, the foam was removed and a new immobilized biomass was grown in the presence of another hydrocarbon. The average hydrocarbon removal efficiency attained was 97%. The organic matter, especially ethanol, was removed with an average efficiency of 83% at a mean influent concentration of 1185.0 mg L-1. A concomitant removal of 97% of nitrate was observed for a mean influent concentration of 423.4 mg L-1. The independent removal of each hydrocarbon demonstrated that these contaminants can be biodegraded separately, without the need for a compound to be the primary substrate for the degradation of another. This study proposes the application of the system for treatment of areas contaminated with these compounds, with substitution and formation of a biofilm in a 30-day period.
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http://dx.doi.org/10.1016/j.jhazmat.2006.06.028DOI Listing
January 2007
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