Publications by authors named "Eun Joo Park"

121 Publications

Thaumatin-like genes function in the control of both biotic stress signaling and ABA signaling pathways.

Biochem Biophys Res Commun 2021 Aug 12;567:17-21. Epub 2021 Jun 12.

Department of Bio-Health Convergence, Duksung Women's University, Seoul, 01369, Republic of Korea; Department of Biotechnology, Duksung Women's University, Seoul, 01369, Republic of Korea. Electronic address:

Thaumatin was isolated as a sweet-tasting protein. Arabidopsis has over 20 Thaumatin-Like Protein (TLP)/Osmoti-Like Protein (OLP) genes that belong to the PR5 family. Although biotic stress-related functions of TLPs have been reported from transgenic lines expressing TLPs, it is nonetheless necessary to investigate genetic phenotypes produced by defects in the TLP genes. In this report, four TLP genes were selected based on sequence similarities (Thau1/2/3/4), and the corresponding mutant thau1/2/3/4 was examined for biotic and abiotic stress responses. The thau1/2/3/4 mutant showed increased susceptibility to the Pseudomonas syringae pv. tomato DC3000 infection, and reduced sensitivity to the ABA and drought stress treatments. Each of the four thaumatin genes showed different gene expression patterns for ABA treatment. Moreover, ABA-inductions of Thau1/2/3/4 were largely dependent on the intact ABA signaling pathway mediated by PYR/PYL receptors. Among the many ABA-responsive genes affected by the defects of Thau1/2/3/4, reduced expression of P5CS1 with decreased accumulation phenotype of prolines indicates that compromised proline synthesis may be associated with the stress phenotypes of thau1/2/3/4. Our data suggest that Thau1/2/3/4 have a function in both biotic stress and abiotic stress signal transduction through the regulation of proline synthesis.
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http://dx.doi.org/10.1016/j.bbrc.2021.06.012DOI Listing
August 2021

A Case Report of Secondary Syphilis with Eosinophilic Spongiosis in an Human Immunodeficiency Virus Patient.

Ann Dermatol 2021 Jun 4;33(3):302-303. Epub 2021 May 4.

Department of Dermatology, Hallym University Sacred Heart Hospital, Anyang, Korea.

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http://dx.doi.org/10.5021/ad.2021.33.3.302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137326PMC
June 2021

A Case of Herpes Zoster Granulomatous Dermatitis: Report of Wolf's Isotopic Response.

Ann Dermatol 2021 Apr 8;33(2):186-189. Epub 2021 Mar 8.

Department of Dermatology, Hallym University Sacred Heart Hospital, Anyang, Korea.

Wolf's isotopic response refers to the occurrence of a novel skin disease at the site of a preceding treated or untreated skin disease. Although the most common preceding skin disease was found to be herpes zoster (HZ), HZ-related dermatological phenomena are not well known in the literature. We report a case of HZ granulomatous dermatitis in a 77-year-old female with a previous history of hypertension, diabetes mellitus, chronic kidney disease, and HZ. She presented with a 3-month history of a pruritic skin lesion on her right thigh. The location of the lesion was consistent with a previous HZ site. Histopathological examination revealed lympho- histiocytic infiltration in the superficial dermis, forming a granulomatous structure. Based on clinical and histopathological findings, we made a diagnosis of granulomatous dermatitis at a previous HZ site. We assumed that the lesion arose from an isotopic response of Wolf. The patient was treated with topical steroids for 3 months and showed clearance of the lesion and symptom. We suggest that treatment should be based on the individual disease, which in our case was topical steroid.
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http://dx.doi.org/10.5021/ad.2021.33.2.186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081995PMC
April 2021

A Rare Case of Cutaneous T-Cell Lymphoma Accompanied by Acute Monoblastic Leukemia and Diffuse Large B-Cell Lymphoma.

Ann Dermatol 2021 Apr 8;33(2):178-181. Epub 2021 Mar 8.

Department of Dermatology, Hallym University Sacred Heart Hospital, Anyang, Korea.

A 70-year-old female was referred for brown-to-gray colored papules and nodules on her lower legs. She had been diagnosed with diffuse large B-cell lymphoma (DLBCL) in her stomach, and myelodysplastic syndrome (MDS) by bone marrow biopsy. Three years after complete remission of DLBCL, she experienced DLBCL recurrence in her small bowel and was hospitalized. MDS had been stationary, but during the treatment of DLBCL, her laboratory findings suggested signs of leukemia. Bone marrow biopsy was done, and acute monoblastic leukemia (AMoL) was diagnosed. After 1 cycle of chemotherapy for AMoL, skin lesions developed, and her skin biopsy showed cutaneous T-cell lymphoma (CTCL). Terminal deoxynucleotidyl transferase staining and CD123 staining were negative, and bone marrow re-biopsy conducted after the skin lesion developed still showed monoblastic proliferation. Whether the CTCL represented with an AMoL lineage switch could not be completely proved due to the absence of molecular or clonal marker evaluations, but the possibility of coexistence of three different malignancies was higher. During treatment, a neutropenic fever developed, and the patient died due to sepsis. We herein report a rare case of CTCL accompanied by AmoL and DLBCL.
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http://dx.doi.org/10.5021/ad.2021.33.2.178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082011PMC
April 2021

A Case of Generalized Lichen Sclerosus et Atrophicus.

Ann Dermatol 2020 Aug 30;32(4):327-330. Epub 2020 Jun 30.

Department of Dermatology, College of Medicine, Hallym University, Anyang, Korea.

A 62-year-old female, with previous history of asthma and hypertension, presented with generalized hyperpigmented skin lesion, found a year ago. Physical examination revealed brown colored lichenified and sclerotic patches on the lower abdomen and flexural areas of extremities. Punch biopsy was performed and histopathological examination revealed hyperkeratosis, follicular plugging and thinning in epidermis. In dermoepidermal junction, cleft like space separating atrophic epidermis and dermis was seen. Also, lichenoid lymphocytic infiltration was observed in mid-dermis. Based on clinical and histopathological findings, a diagnosis of generlaized lichen sclerosus et atrophicus (LSA) was made. Other laboratory examinations were unremarkable. As there is no standard treatment for LSA, the patient received various treatments including topical steroid, tacrolimus and narrow-band ultraviolet B therapy. The skin lesion has softened and its color improved after treatment. LSA is defined as infrequent chronic inflammatory dermatosis with anogenital and extragenital manifestations. Generalized type is rare and genital involvement is the most frequent and often the only site of involvement. We report this case as it is an uncommon type of LSA with generalized hyperpigmented and sclerotic skin lesion in a postmenopausal female patient.
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http://dx.doi.org/10.5021/ad.2020.32.4.327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992656PMC
August 2020

A Case of Solitary Fibrous Tumor of Subungual Region.

Ann Dermatol 2020 Apr 11;32(2):146-150. Epub 2020 Mar 11.

Department of Dermatology, Hallym University Sacred Heart Hospital, Anyang, Korea.

Solitary fibrous tumor (SFT) is a relatively uncommon mesenchymal neoplasm that usually arises in the pleura, but also has been reported in numerous extrapleural locations, including cutaneous site. The skin lesion presents as a circumscribed nodule or tumor, mainly on the head and neck. A 41-year-old male presented with 6 months history of nail lesion without symptom on the left third finger. The lesion is slightly yellowish discoloration with subungual erythematous nodule and distal onycholysis. Biopsy specimen from the nail lesion showed the spindle cells form patternless pattern with hypercellular and hypocellular area. And small blood vessels and dilated vascular spaces were present. The result of special stain for specimen showed that positive for CD34, Bcl-2, and CD99 but negative for S-100, FactorXIIIa, and smooth muscle action. Recognition of this uncommon location of SFT is important because of possible confusion with other subungual tumors, including glomus tumor, fibroma and other fibrohistiocytic tumors like dermatofibrosarcoma protuberans, superficial acral fibromyxoma and cellular digital fibroma. Here in, we report a case of SFT of subungual region. We think this case is interesting because of uncommon location and may be helpful to more understand the character of this disease.
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http://dx.doi.org/10.5021/ad.2020.32.2.146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992551PMC
April 2020

Synergistically integrated phosphonated poly(pentafluorostyrene) for fuel cells.

Nat Mater 2021 Mar 7;20(3):370-377. Epub 2020 Dec 7.

MPA-11: Materials Synthesis and Integrated Devices, Los Alamos National Laboratory, Los Alamos, NM, USA.

Modern electrochemical energy conversion devices require more advanced proton conductors for their broad applications. Phosphonated polymers have been proposed as anhydrous proton conductors for fuel cells. However, the anhydride formation of phosphonic acid functional groups lowers proton conductivity and this prevents the use of phosphonated polymers in fuel cell applications. Here, we report a poly(2,3,5,6-tetrafluorostyrene-4-phosphonic acid) that does not undergo anhydride formation and thus maintains protonic conductivity above 200 °C. We use the phosphonated polymer in fuel cell electrodes with an ion-pair coordinated membrane in a membrane electrode assembly. This synergistically integrated fuel cell reached peak power densities of 1,130 mW cm at 160 °C and 1,740 mW cm at 240 °C under H/O conditions, substantially outperforming polybenzimidazole- and metal phosphate-based fuel cells. Our result indicates a pathway towards using phosphonated polymers in high-performance fuel cells under hot and dry operating conditions.
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http://dx.doi.org/10.1038/s41563-020-00841-zDOI Listing
March 2021

Differential histopathological and immunohistochemical findings between palmar psoriasis and chronic hand eczema.

Eur J Dermatol 2020 Dec;30(6):710-715

Department of Dermatology.

Background: The differential diagnosis between palmar psoriasis (PP), chronic hand dermatitis (CHE), and hyperkeratotic hand dermatitis (HHD) is challenging.

Objectives: We sought to distinguish the histopathological and immunohistochemical characteristics between PP, CHD, and HHD.

Materials & Methods: Hands, clinically diagnosed with PP, CHD, or HHD, were further evaluated using skin biopsy sections based on haematoxylin and eosin staining and immunohistochemical analysis for β-defensin 2 and interleukin-36γ.

Results: Confluent parakeratosis, absent granular layer, and psoriasiform epidermal hyperplasia were more common in PP and HHD relative to CHE. The level of β-defensin 2 expression in the stratum corneum and interleukin-36γ in the stratum granulosum was higher in PP and HHD relative to CHD.

Conclusion: Considering the similarities of histopathological and immunohistochemical findings, HHD may be an inflammatory disorder with a pathogenesis similar to that of PP, rather than CHD.
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http://dx.doi.org/10.1684/ejd.2020.3769DOI Listing
December 2020

Histological and immunohistopathological differentiation between guttate psoriasis and pityriasis rosea.

Australas J Dermatol 2020 Nov 6;61(4):e481-e484. Epub 2020 Jul 6.

Department of Dermatology, Hallym University Sacred Heart Hospital, Anyang, Korea.

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http://dx.doi.org/10.1111/ajd.13370DOI Listing
November 2020

Improvement of glymphatic-lymphatic drainage of beta-amyloid by focused ultrasound in Alzheimer's disease model.

Sci Rep 2020 09 30;10(1):16144. Epub 2020 Sep 30.

Department of Nuclear Medicine, College of Medicine, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 03080, South Korea.

Drainage of parenchymal waste through the lymphatic system maintains brain homeostasis. Age-related changes of glymphatic-lymphatic clearance lead to the accumulation beta-amyloid (Aβ) in dementia models. In this study, focused ultrasound treatment in combination with microbubbles (FUS-MB) improved Aβ drainage in early dementia model mice, 5XFAD. FUS-MB enhanced solute Aβ clearance from brain, but not plaques, to cerebrospinal fluid (CSF) space and then deep cervical lymph node (dCLN). dCLN ligation exaggerated memory impairment and progress of plaque formation and also the beneficial effects of FUS-MB upon Aβ removal through CSF-lymphatic routes. In this ligation model, FUS-MB improved memory despite accumulation of Aβ in CSF. In conclusion, FUS-MB enhances glymphatic-lymphatic clearance of Aβ mainly by increasing brain-to-CSF Aβ drainage. We suggest that FUS-MB can delay dementia progress in early period and benefits of FUS-MB depend on the effect of Aβ disposal through CSF-lymphatics.
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http://dx.doi.org/10.1038/s41598-020-73151-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527457PMC
September 2020

Clinical significance of high on-treatment platelet reactivity in patients with prolonged clopidogrel therapy.

Korean J Intern Med 2021 03 25;36(Suppl 1):S80-S89. Epub 2021 Feb 25.

Division of Cardiology, Department of Internal Medicine, Hallym University Kangnam Sacred Heart Hospital, Seoul, Korea.

Background/aims: Prolonged dual antiplatelet therapy (DAPT) with aspirin and clopidogrel beyond 1 year has been shown to reduce ischemic events at the expense of increased bleeding. However, limited data are available on the clinical significance of platelet reactivity (PR) at 1 year.

Methods: We retrospectively identified 331 patients who underwent percutaneous coronary intervention (PCI) and assessed the on-clopidogrel PR using VerifyNow P2Y12 assay at 1 year in a single center. Two hundred eleven patients were on DAPT for > 1 year. The relationship between high on-treatment platelet reactivity (HPR) at 1 year and clinical outcomes beyond 1 year, as well as the longitudinal change in PR was analyzed.

Results: At 1 year, 135 (64%) patients showed HPR and 76 (36%) did not. There was a significant increase in ischemic endpoint events, including cardiovascular death, non-fatal myocardial infarction, and stroke/transient ischemic attack in patients with compared to without HPR at 1 year (hazard ratio [HR], 2.68; 95% confidence interval [CI], 1.06 to 6.77; p = 0.036). However, the incidence of any Bleeding Academic Research Consortium bleeding was significantly lower in the HPR group (HR, 0.11; 95% CI, 0.02 to 0.65; p = 0.015). In the longitudinal analysis, PR significantly decreased from post-load to 1 year after index PCI in the non-HPR group. Conversely, the HPR group showed high PR from baseline through 1 year.

Conclusion: HPR at 1 year may be a useful surrogate for predicting ischemic and bleeding events in patients on prolonged DAPT. Patients with and without HPR at 1 year showed different patterns of longitudinal change in PR.
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http://dx.doi.org/10.3904/kjim.2020.085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009166PMC
March 2021

Comparison of the Diagnostic Performance of Strain Elastography and Shear Wave Elastography for the Diagnosis of Carpal Tunnel Syndrome.

J Ultrasound Med 2021 May 27;40(5):1011-1021. Epub 2020 Aug 27.

Department of Radiology, Inje University College of Medicine, Haeundae Paik Hospital, 875 Haeundae-ro, Haeundae-gu, Busan, 48108, Republic of Korea.

Objectives: To compare the diagnostic performance between strain elastography and shear wave elastography (SWE) for the diagnosis of carpal tunnel syndrome (CTS).

Methods: Between July 2018 and June 2019, 66 consecutive patients with 95 imaged wrists underwent wrist ultrasound, including grayscale ultrasound, strain elastography, and SWE, because of the suspicion of CTS. During wrist ultrasound, the cross-sectional area (CSA), strain ratio, elasticity, and shear wave velocity of each median nerve were measured at the proximal carpal bone level (scaphoid to pisiform). The variables were compared between the normal and CTS groups by using the independent t test, and subgroup analyses were performed using one-way analysis of variance. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of each variable.

Results: CSA, elasticity, and shear wave velocity showed significant intergroup differences (P < 0.001, P < 0.001, and P = 0.002, respectively). However, the strain ratio showed no statistically significant intergroup difference (P = 0.639). In the subgroup analyses, elasticity showed significantly higher values in the severe group than in the mild and moderate groups (P < 0.001 and P = 0.001, respectively). Other parameters showed no significant differences among the different subgroups. The areas under the ROC curve were 0.823 for CSA, 0.772 for elasticity, and 0.779 for shear wave velocity. The differences in the areas under the ROC curve among CSA, elasticity, and shear wave velocity were not statistically significant (all P > 0.05).

Conclusions: SWE has a good diagnostic value in CTS. In particular, elasticity can discriminate the severe group from the other groups.
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http://dx.doi.org/10.1002/jum.15478DOI Listing
May 2021

Synergistic Effects of Pulsed Focused Ultrasound and a Doxorubicin-Loaded Microparticle-Microbubble Complex in a Pancreatic Cancer Xenograft Mouse Model.

Ultrasound Med Biol 2020 11 20;46(11):3046-3058. Epub 2020 Aug 20.

Department of Radiology and Institute of Radiation Medicine, Seoul National University Hospital, Seoul, Korea; Department of Radiology, Seoul National University College of Medicine, Seoul, Korea.

The synergistic effects of a doxorubicin (Dox)-loaded microparticle-microbubble complex (DMMC) and focused ultrasound (FUS) with a short duty cycle (5%) were evaluated in a pancreatic cancer xenograft model established by inoculating immunodeficient mice with CFPAC-1 cells. The efficacy of the DMMC with FUS (study 1), the effect of conjugating the particles as opposed to mixing them (study 2) and the levels of tumor apoptosis and intracellular Dox (study 3) were evaluated. The DMMC with FUS exhibited the lowest tumor growth rate (30.8 mm/wk) and the highest intracellular Dox uptake (8.8%) and tumor cell apoptosis rate (58.7%) among all treatments. DMMC had a significantly lower growth rate than the mixture of Dox-loaded microparticles and microbubbles (44.2 mm/wk, p < 0.01) when they were combined with FUS. In conclusion, DMMC with short-duty-cycle FUS holds promise for tumor growth suppression, which may be attributed to high intracellular Dox uptake.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2020.07.019DOI Listing
November 2020

Texture analysis on bi-parametric MRI for evaluation of aggressiveness in patients with prostate cancer.

Abdom Radiol (NY) 2020 12 1;45(12):4214-4222. Epub 2020 Aug 1.

Department of Radiology, Haeundae Paik Hospital, Inje University College of Medicine, Haeundae-ro 875, Haeundae-gu, Busan, 48108, Korea.

Purpose: To evaluate the association between texture parameters based on bi-parametric MRI and Gleason score (GS) in patients with prostate cancer (PCa) and to evaluate diagnostic performance of any significant parameter for discriminating clinically significant cancer (CSC, GS ≥ 7) from non-CSC.

Methods: A total of 116 patients who had been confirmed as prostate adenocarcinoma by radical prostatectomy or biopsy were divided into a training (n = 65) and a validation dataset (n = 51). All of the patients underwent preoperative 3T-MRI. Texture analysis was performed on axial T2WI and ADC maps (generated from b values, 0 and 1000 s/mm) using dedicated software to cover the whole tumor volume. The correlation coefficient was calculated to evaluate the association between texture parameters and GS, and subsequent multiple regression analyses were applied for the significant parameters. To extract an optimal cut-off value for prediction of CSC, ROC curve analysis was performed.

Results: In the training dataset, gray-level co-occurrence matrix (GLCM) entropy on ADC map was the only significant indicator for GS (coefficient of determination R, 0.4227, P = 0.0034). The AUC of GLCM entropy on ADC map was 0.825 (95% CI 0.711-0.907) with a maximum accuracy of 82%, a sensitivity of 86%, a specificity of 71%. When a cut-off value of 2.92 was applied to the validation dataset, it showed an accuracy of 92%, a sensitivity of 98%, and a specificity of 70%.

Conclusion: GLCM entropy on ADC map was associated with GS in patients with PCa and its estimated accuracy for discriminating CSC from non-CSC was 82%.
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http://dx.doi.org/10.1007/s00261-020-02683-4DOI Listing
December 2020

Efficacy and Safety of Treatment with Oral Alitretinoin and Oral Cyclosporine for Chronic Hand Eczema: A Retrospective Review of 118 Cases.

Acta Derm Venereol 2020 Jan 30;100(1):adv00043. Epub 2020 Jan 30.

Department of Dermatology, Hallym University Sacred Heart Hospital, 14068 Anyang, Korea.

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http://dx.doi.org/10.2340/00015555-3392DOI Listing
January 2020

Portable ultrasound-guided high-intensity focused ultrasound with functions for safe and rapid ablation: prospective clinical trial for uterine fibroids-short-term and long-term results.

Eur Radiol 2020 Mar 8;30(3):1554-1563. Epub 2019 Nov 8.

Department of Radiology and the Institute of Radiation Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.

Objectives: To investigate the efficacy and safety of a new portable ultrasound-guided high-intensity focused ultrasound system (USgHIFU) with advanced targeting and beam steering technology for the treatment of uterine fibroids.

Methods: Fifty-nine uterine fibroids from 36 participants (mean age, 44.9 ± 4.1 years) were included from November 2013 to November 2015. All participants were treated with HIFU, with 3D electronic steering. MR imaging studies were performed before HIFU, immediately after HIFU, and 1 month and 3 (or 5) months after the HIFU treatment. The non-perfused volume ratio (NPVR), fibroid volume shrinkage (FVS), symptom improvement, quantified life quality assessment, and safety were analyzed. A long-term follow-up was conducted in July to December 2017 (mean, 32.2 months).

Results: The volume of the treated uterine fibroids ranged from 7.5 to 274.4 cm (mean, 69.8 cm; SD, 64.3 cm). The mean NPVR on the immediate post-HIFU MR imaging was 74.8 ± 25.2%. The mean FVS was 17.3% at 1 month, 33.3% at 3 months, and 45.1% at 5 months after HIFU treatment. The mean treatment time was 44.6 ± 28.2 min per fibroid and 72.9 ± 31.4 min per participant. Uterine fibroid-related symptoms and quality of life showed statistically significant improvement after the HIFU treatment. No significant symptoms related to safety or complications occurred. In the long-term follow-up, 78.8% of those surveyed were satisfied with their HIFU treatment.

Conclusion: This clinical trial showed that a portable USgHIFU with advanced functions may safely and effectively treat uterine fibroids.

Key Points: • A portable compact ultrasound-guided high-intensity focused ultrasound (HIFU) can effectively and safely treat uterine fibroids. • Advanced functions, such as portability, targeted forecasting, electronic beam steering, and interleaved scanning, might be helpful in enhancing the clinical applicability of ultrasound-guided high-intensity focused ultrasound. • In the long-term follow-up of more than 2 years, approximately 80% of those surveyed were satisfied with their HIFU treatment.
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http://dx.doi.org/10.1007/s00330-019-06468-2DOI Listing
March 2020

Appropriate Timing of Early Postoperative Botulinum Toxin Type A Injection for Thyroidectomy Scar Management: A Split-Scar Study.

Plast Reconstr Surg 2019 10;144(4):659e-668e

From the Department of Dermatology and the Division of Breast and Endocrine Surgery, College of Medicine, Hallym University.

Background: Botulinum toxin type A (BTxA) injection is effective for surgical scar prevention. Although some studies have aimed to confirm the efficacy of BTxA injection at different time points, none has been conducted to determine the most appropriate timing of injection for scar management. The authors predicted that the injection of BTxA at different times during the wound healing process would cause differing scar quality improvement and clarify unknown molecular mechanisms.

Methods: The study included adults who underwent thyroidectomy. All patients received paralesional BTxA injections on the day of the surgery on either the right or left side of the operative site. The same dose was injected on the noninjected side by means of the same method after 2 weeks. At 2, 4, 12, and 24 weeks postoperatively, the modified Stony Brook Scar Evaluation Scale, visual analogue scale, and erythema index were used for objective, subjective, and quantitative evaluations of the scar. At week 24 postoperatively, a quantitative scar assessment was performed with respect to the erythema index, skin elasticity, melanin index, and friction.

Results: On objective evaluation of the scar and patient satisfaction at 24 weeks postoperatively, the operation-day injection side showed better outcomes than the 2-week-postoperative injection side. These differences were significant from postoperative week 4. In the final quantitative scar assessment at postoperative week 24, significant improvements were observed in the erythema index and skin elasticity.

Conclusion: These results suggest that immediate postoperative BTxA injection is more effective for thyroidectomy scar management in terms of erythema, skin elasticity, and patient satisfaction.

Clinical Question/level Of Evidence: Therapeutic, II.
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http://dx.doi.org/10.1097/PRS.0000000000006064DOI Listing
October 2019

Botulinum toxin type A suppresses pro-fibrotic effects via the JNK signaling pathway in hypertrophic scar fibroblasts.

Arch Dermatol Res 2019 Dec 9;311(10):807-814. Epub 2019 Sep 9.

Department of Dermatology, Hallym University Sacred Heart Hospital, 22 Gwanpyeong-ro 170 Beon-gil, Dongan-gu, Anyang, Gyeonggi-do, 14068, South Korea.

Hypertrophic scar is a dermal fibroproliferative disease characterized by the overproduction and deposition of extracellular matrix, and the hyperproliferation and enhanced angiogenesis of fibroblasts, along with their enhanced differentiation to myofibroblasts. Botulinum toxin type A shows potential for prevention of hypertrophic scar formation; however, its effectiveness in attenuating skin fibrosis and the related mechanism are unclear. In this study, human scar fibroblasts were cultured and stimulated with botulinum toxin type A, and the changes in fibroblast proliferation, migration, and protein expression of pro-fibrotic factors were evaluated with colorimetric, scratch, and enzyme-linked immunosorbent assays and western blotting, respectively. Botulinum toxin type A treatment decreased the proliferation and migration of human scar fibroblasts compared with those of untreated controls. Protein expression levels of pro-fibrotic factors (transforming growth factor β1, interleukin-6, and connective tissue growth factor) were also inhibited by botulinum toxin type A, whereas the JNK phosphorylation level was increased. Activation of the JNK pathway demonstrated the inhibitory effects of the toxin on human scar fibroblast proliferation and production of pro-fibrotic factors, suggesting that the suppressive effects of botulinum toxin type A are closely associated with JNK phosphorylation. Overall, this study showed that botulinum toxin type A has a suppressive effect on extracellular matrix production and scar-related factors in human scar fibroblasts in vitro, and that regulation of JNK signaling plays an important role in this process. Our results provide a theoretical basis, at the cellular level, for the therapeutic use of botulinum toxin type A.
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http://dx.doi.org/10.1007/s00403-019-01975-0DOI Listing
December 2019

Clinicopathological differentiation between Pityrosporum folliculitis and acneiform eruption.

J Dermatol 2019 Nov 5;46(11):978-984. Epub 2019 Sep 5.

Department of Dermatology, College of Medicine, Hallym University, Anyang, Korea.

Distinguishing between Malassezia folliculitis (Pityrosporum folliculitis [P. folliculitis]) and acneiform eruption, based on clinicopathological features, is challenging for clinicians. In the literature, the histopathological differences between P. folliculitis and acneiform eruption lesions have been poorly described. We aimed to determine the clinicopathologic distinctions between P. folliculitis and acneiform eruption by retrospectively analyzing the histology of hematoxylin and eosin stained tissue sections obtained from 52 patients diagnosed with these lesions. The presence of fungal spores in the follicular lumen was most consistent with a P. folliculitis diagnosis (P < 0.001). However, intrafollicular inflammation (P = 0.009), irregular patterns of keratin plugging (P = 0.008), and nuclear dust in the follicular lumen (P < 0.001) favored an acneiform eruption diagnosis. These intrafollicular characteristics and inflammatory differences are believed to be caused by necrotic keratinocytes that lead to vacuolar changes in the follicular wall (P = 0.013). We did not observe any difference between P. folliculitis and acneiform eruption lesions in terms of perifollicular inflammatory cell infiltration. Our study demonstrated that significant differences exist between P. folliculitis and acneiform eruption lesions relative to the presence of necrotic keratinocytes in the follicular wall, intrafollicular characteristics, and inflammatory cell infiltrations. Necrotic keratinocytes are believed to have a key role in these differences. These findings may contribute to an improved understanding of the pathogenesis and differential diagnosis of P. folliculitis and acneiform eruption.
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http://dx.doi.org/10.1111/1346-8138.15070DOI Listing
November 2019

Correlation of constitutive photomorphogenic 1 (COP1) and p27 tumor suppressor protein expression in ovarian cancer.

Genes Genomics 2019 08 26;41(8):879-884. Epub 2019 Apr 26.

Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea.

Background: Constitutive photomorphogenic 1 (COP1) is an E3 ubiquitin ligase that regulates important target proteins for cell growth including p27. The tumor suppressor p27 negatively regulates the cell cycle by inhibiting cyclin-dependent kinase. COP1 negatively regulates p27 stability by mediating its nuclear export and degradation.

Objective: Even if COP1 and p27 are tightly related and have significant roles in tumor progression, the expression patterns and relationship of both proteins in cancer have not yet been studied.

Method: We analyzed the expression patterns and relationship between COP1 and p27 using an ovarian cancer tissue microarray by dual immunofluorescence analysis.

Results: The expression levels of COP1 and p27 proteins were not significantly different between ovarian cancer tissue and normal control tissue. Other clinical data including age, tumor type, tumor grade, and stage were not significantly related to expression of the two proteins. The co-relationship between COP1 and p27 proteins was significantly high (Pearson correlation coefficient 0.79, p = 8.65 × 10).

Conclusions: Our results demonstrate that while the expression levels of COP1 and p27 are highly correlated, they are not significantly related to cancer progression in ovarian cancer.
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http://dx.doi.org/10.1007/s13258-019-00818-6DOI Listing
August 2019

Structural determinants for pyrabactin recognition in ABA receptors in Oryza sativa.

Plant Mol Biol 2019 Jun 2;100(3):319-333. Epub 2019 Apr 2.

Department of Biological Sciences, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

Key Message: We determined the structure of OsPYL/RCAR3:OsPP2C50 complex with pyrabactin. Our results suggest that a less-conserved phenylalanine of OsPYL/RCAR subfamily I is one of considerations of ABA agonist development for Oryza sativa. Pyrabactin is a synthetic chemical mimicking abscisic acid (ABA), a naturally occurring phytohormone orchestrating abiotic stress responses. ABA and pyrabactin share the same pocket in the ABA receptors but pyrabactin modulates ABA signaling differently, exhibiting both agonistic and antagonistic effects. To explore structural determinants of differential functionality of pyrabactin, we determined the crystal structure of OsPYL/RCAR3:pyrabactin:OsPP2C50, the first rice ABA receptor:co-receptor complex structure with a synthetic ABA mimicry. The water-mediated interaction between the wedging Trp-259 of OsPP2C50 and pyrabactin is lost, undermining the structural integrity of the ABA receptor:co-receptor. The loss of the interaction of the wedging tryptophan of OsPP2C with pyrabactin appears to contribute to the weaker functionality of pyrabactin. Pyrabactin in the OsPYL/RCAR3:OsPP2C50 complex adopts a conformation different from that in ABA receptors from Arabidopsis. Phe125, specific to the subfamily I of OsPYL/RCARs in the ABA binding pocket, appears to be the culprit for the differential conformation of pyrabactin. Although the gate closure essential for the integrity of ABA receptor:co-receptor is preserved in the presence of pyrabactin, Phe125 apparently restricts accessibility of pyrabactin, leading to decreased affinity for OsPYL/RCAR3 evidenced by phosphatase assay. However, Phe125 does not affect conformation and accessibility of ABA. Yeast two-hybrid, germination and gene transcription analyses in rice also support that pyrabactin imposes a weak effect on the control of ABA signaling. Taken together, our results suggest that phenylalanine substitution of OsPYL/RCARs subfamily I may be one of considerations for ABA synthetic agonist development.
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http://dx.doi.org/10.1007/s11103-019-00862-6DOI Listing
June 2019

Phenyl Oxidation Impacts the Durability of Alkaline Membrane Water Electrolyzer.

ACS Appl Mater Interfaces 2019 Mar 4;11(10):9696-9701. Epub 2019 Mar 4.

MPA-11: Materials Synthesis & Integrated Devices , Los Alamos National Laboratory , Los Alamos , New Mexico 87545 , United States.

The durability of alkaline anion exchange membrane (AEM) electrolyzers is a critical requirement for implementing this technology in cost-effective hydrogen production. Here, we report that the electrochemical oxidation of the adsorbed phenyl group (found in the ionomer) on oxygen evolution catalysts produces phenol, which may cause performance deterioration in AEM electrolyzers. In-line H NMR kinetic analyses of phenyl oxidation in a model organic cation electrolyte shows that catalyst type significantly impacts the phenyl oxidation rate at an oxygen evolution potential. Density functional theory calculations show that the phenyl adsorption is a critical factor determining the phenyl oxidation. This research provides a path for the development of more durable AEM electrolyzers with components that can minimize the adverse impact induced by the phenyl group oxidation, such as the development of novel ionomers with fewer phenyl moieties or catalysts with less phenyl-adsorbing character.
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http://dx.doi.org/10.1021/acsami.9b00711DOI Listing
March 2019

Two Cases of Infective Endocarditis in Patients with Atopic Dermatitis.

Ann Dermatol 2019 Feb 2;31(1):70-74. Epub 2019 Jan 2.

Department of Dermatology, Hallym University Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, Korea.

Patients with atopic dermatitis have high rates of skin surface colonization of . At the same time, is the major causative organism in infective endocarditis, approximately accounting for 30%~50% cases of infective endocarditis. A 22-year-old male with severe atopic dermatitis presented with fever and myalgia. He was diagnosed with active infective endocarditis causing multiple cerebral infarction, splenic infarction, and septic shoulder requiring synovectomy. Blood culture proved methicillinsensitive bacteremia, and the culture from the skin revealed same bacteria. After treated with intravenous antibiotics for 6 weeks, patient was improved. Another 42-year-old female with severe atopic dermatitis who presented with fever and chilling was hospitalized due to acute infective endocarditis. She also had left flank pain and visual disturbance, due to splenic infarction and acute cerebral infarction, respectively. As blood culture revealed methicillin-sensitive bacteremia, she treated with intravenous antibiotics for 6 weeks. The route of entry of two patients was attributed to the patient eczematous scratching lesion of poorly controlled atopic dermatitis. Infective endocarditis can result in the context of acute deterioration of atopic dermatitis. Dermatologists need to pay attention to this risk and actively manage such conditions in order to decrease the risk of infective endocarditis arising from skin lesions in atopic patients. For these reasons, we herein report two cases of infective endocarditis in patients with atopic dermatitis.
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http://dx.doi.org/10.5021/ad.2019.31.1.70DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992700PMC
February 2019

Increasing Prevalence of the Sensitization to Cat/Dog Allergens in Korea.

Ann Dermatol 2018 Dec 26;30(6):662-667. Epub 2018 Oct 26.

Department of Dermatology, Hallym University Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, Korea.

Background: Recently, the number of domestic pets has increased. As a consequence, sensitization to animal allergens, such as cat or dog allergens, has become a problem.

Objective: We studied the annual trends of sensitization to cats or dogs, and the characteristics of the patients.

Methods: We retrospectively analyzed the medical records of 7,469 patients who visited a dermatology clinic and underwent an allergic profile test, from January 2011 to December 2015. Specific immunoglobulin E (IgE) levels to cat or dog antibody greater than 3.50 IU/ml were regarded as positive results.

Results: In all, 274 patients showed significant increase in levels of specific IgE antibody to dog, and 307 revealed increase in levels of of specific IgE antibody to cat. The prevalence of these specific IgEs increased from 2011 to 2015. Independent risks for sensitization to cat allergens were sensitization to dog, but not to house dust, , and . Independent risks for sensitization to dog allergens were sensitization to cat, but not to house dust, , and . Total IgE level was not related to specific IgE level against either cats or dogs.

Conclusion: In conclusion, the prevalence of sensitization to cat or dog has increased. Sensitization to cat or dog is related to each other, but is irrelevant to the total IgE level.
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http://dx.doi.org/10.5021/ad.2018.30.6.662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992440PMC
December 2018

Effect of platelet-rich plasma on proliferation and migration in human dermal fibroblasts.

J Cosmet Dermatol 2019 Aug 2;18(4):1105-1112. Epub 2018 Oct 2.

Department of Dermatology, College of Medicine, Hallym University, Anyang, Korea.

Background: Platelet-rich plasma (PRP) is a blood fraction that contains high concentrations of several growth factors. PRP has been recently used in skin wound healing and rejuvenation. However, the precise molecular mechanisms underlying PRP-induced wound healing are unknown.

Aims: This study aimed to evaluate the effects of PRP on extracellular matrix remodeling, which requires the activation of dermal fibroblasts.

Methods: Cell proliferation and migration assay, enzyme-linked immunosorbent analysis, and Western blotting were performed on PRP-treated human skin fibroblasts.

Results: Platelet numbers were enhanced by 4.6-fold in PRP compared to that in whole blood. PRP stimulated the proliferation and migration of human dermal fibroblasts and increased the expression of human procollagen I alpha 1, elastin, MMP-1, and MMP-2 in human dermal fibroblasts. PRP-treated human dermal fibroblasts also showed a dramatic reduction in the phosphorylation of c-Jun N-terminal kinase (JNK), whereas total JNK levels were not significantly reduced.

Conclusions: Collectively, PRP induced increased expression of type I collagen, elastin, MMP-1, and MMP-2, thereby accelerating wound healing. Our findings reveal basic mechanisms underlying PRP-mediated tissue remodeling. Thus, these results could be exploited for clinical dermatology and skin rejuvenation.
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http://dx.doi.org/10.1111/jocd.12780DOI Listing
August 2019

Development and evaluation of a CEACAM6-targeting theranostic nanomedicine for photoacoustic-based diagnosis and chemotherapy of metastatic cancer.

Theranostics 2018 30;8(15):4247-4261. Epub 2018 Jul 30.

Department of Chemical & Biomolecular Engineering, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul, 121-742, Republic of Korea.

Metastasis is the leading cause of cancer-related deaths. A number of chemotherapeutic and early diagnosis strategies, including nanomedicine, have been developed to target metastatic tumor cells. However, simultaneous inhibition and imaging of metastasis is yet to be fully achieved. To overcome this limitation, we have developed human serum albumin-based nanoparticles (tHSA-NPs) with photoacoustic imaging capability, which target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6). CEACAM6 is highly expressed in metastatic anoikis-resistant tumor cells. , the CEACAM6-targeting tHSA-NPs efficiently targeted CEACAM6-overexpressing metastatic anoikis-resistant tumor cells. , CEACAM6-targeting tHSA-NPs administered intravenously to BALB/c nude mice efficiently inhibited lung metastasis in circulating anoikis-resistant tumor cells compared to the controls. In addition, anoikis-resistant tumor cells can be successfully detected by photoacoustic imaging, both and using the intrinsic indocyanine green-binding affinity of albumin. In summary, the CEACAM6-targeting albumin-based nanoparticles allowed the delivery of drugs and photoacoustic imaging to metastatic anoikis-resistant tumor cells and . Based on the expression of CEACAM6 in a variety of tumors, CEACAM6-targeting nanomedicine might be used to target various types of metastatic tumor cells.
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http://dx.doi.org/10.7150/thno.25131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096393PMC
September 2019

Relationship between Age at Menarche and Metabolic Syndrome in Premenopausal Women: Korea National Health and Nutrition Examination Survey 2013-2014.

Korean J Fam Med 2018 Sep 30;39(5):300-306. Epub 2018 Jul 30.

Department of Family Medicine, Inje University Haeundae Paik Hospital, Busan, Korea.

Background: Early menarche may be associated with increased risk of cardiovascular disease. The aim of this study was to evaluate the relationship between age at menarche and metabolic syndrome (MetS) in Korean premenopausal women.

Methods: We used nationally representative data from the Korea National Health and Nutrition Examination Survey from 2013 to 2014, and 3,023 premenopausal women aged 20-55 years were our subjects. We defined early menarche as age at first menstrual period less than 12 years. Multivariable logistic regression analysis was used to evaluate the relationship between age at menarche and MetS after adjusting for current age, and socioeconomic, lifestyle, and reproductive variables.

Results: MetS was much more common in women aged 40-55 years than in women aged 20-39 years (4.1% vs. 15.1%). Compared with women who experienced menarche at age 12-15 years, the risk of MetS in the early menarche group was not higher in either age group, after adjusting for current age, and socioeconomic, lifestyle, and reproductive variables (odds ratio [OR], 1.767; 95% confidence interval [CI], 0.718-4.351 in those aged 20-39 years; OR, 1.780; 95% CI, 0.775-4.085 in those aged 40-55 years). The risk of MetS in women with menarche at age ≥16 years was not higher than in women with menarche at age 12-15 years.

Conclusion: Early or late menarche was not associated with an increased risk of MetS in premenopausal Korean women. Even before menopause, current age has a major influence on the development of MetS.
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http://dx.doi.org/10.4082/kjfm.17.0022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166116PMC
September 2018

Relationship between Dry Eye Syndrome and Frequency of Coffee Consumption in Korean Adults: Korea National Health and Nutrition Examination Survey V, 2010-2012.

Korean J Fam Med 2018 Sep 27;39(5):290-294. Epub 2018 Jul 27.

Department of Family Medicine, Inje University Haeundae Paik Hospital, Busan, Korea.

Background: Dry eye syndrome is a common health problem in the adult population. Many risk factors including age, sex, prior eye surgery, various chronic diseases, and lifestyle factors can affect its development. We have evaluated the risk of dry eye syndrome based on the frequency of coffee consumption among Korean adult population.

Methods: A total of 9,752 adults with age 19 years and older were randomly selected between 2010 and 2012. They have all participated in the National Health and Nutrition Examination Survey V of Korea. Dry eye syndrome was being diagnosed by the physicians at some points in the participant's lifetime. The average daily coffee intake was divided into the following: less than 1 cup, 1 to 2 cups, and 3 cups or more. Various physio-environmental factors and medical conditions were used as correction variables to assess the risk of dry eye syndrome in relation to the frequency of coffee consumption.

Results: The prevalence of dry eye syndrome decreased to 9.2%, 8.8%, and 6.3% as coffee consumption increased from less than 1 cup to 1-2 cups and more than 3 cups, respectively. However, there was no significant relationship between the frequency of coffee consumption and the risk of dry eye syndrome after adjusting various risk factors.

Conclusion: There is no relationship between the frequency of coffee consumption and risk of dry eye syndrome.
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http://dx.doi.org/10.4082/kjfm.17.0047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166118PMC
September 2018

Longitudinal melanonychia in adults and children: a clinical and histopathological review of Korean patients.

Eur J Dermatol 2018 Jun;28(3):400-401

Department of Dermatology, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Korea.

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http://dx.doi.org/10.1684/ejd.2018.3274DOI Listing
June 2018
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