Publications by authors named "Erica Georgina Wilson"

3 Publications

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inhibitory effect of Lam. extracts over and on different stages of the chlamydial life cycle.

Heliyon 2021 May 18;7(5):e06947. Epub 2021 May 18.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Microbiología Clínica, Ciudad Autónoma de Buenos Aires, Argentina.

Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both and can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over and growth. The inhibitory activity over different stages of the chlamydial life cycle on cell culture was assessed: the entry, the inclusion developing after entry, and the exponential growth stage. Also, the capability of rendering the cell refractory to chlamydial infection by pre-incubation with the extracts was assayed. Inhibitory activity of water-based and organic-based extracts obtained from Lam. (Araliaceae), (Vell.) Engl. (Anacardiaceae) and (Mutis ex L.f.) Baill. (Violaceae) were tested against five strains of (L2/434/BU and four clinical isolates form both neonatal conjunctivitis and adult genital infections, genotypes D, E, and K) and against AR39. The dichloromethane extract showed a broad inhibitory activity over the exponential growth stage of and independently from the chlamydial strain and the cell line. These results suggest a high inhibitory potential on both species. In order to characterize the dichloromethane active extract, an H-NMR was performed. The H-NMR characterization showed a spectrum with characteristic signals of the fatty acid moiety of lipids or cerebrosides, volatile phenolics, phytosterols, methyl triterpenes signals, and glucose moiety of the cerebrosides.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141889PMC
May 2021

Metabolic fingerprinting of banana passion fruits and its correlation with quorum quenching activity.

Phytochemistry 2020 Apr 4;172:112272. Epub 2020 Feb 4.

Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, the Netherlands; College of Pharmacy, Kyung Hee University, 02447, Seoul, Republic of Korea.

Banana passion fruit of the Passiflora genus, are commercially cultivated on a small to medium scale, mainly as edible fruits or as components of traditional herbal medicines. This subgenus comprises several species and hybrid specimens that grow readily in the wild. Due to their taxonomical complexity, many of these species have recently been reclassified (Ocampo Pérez and Coppens d'Eeckenbrugge, 2017), and their chemical profile has still to be determined. In this study, an H NMR-based platform was applied to the chemical profiling of seven wild species of the Passiflora subgenus, and UHPLC-DAD-MS was additionally used for the identification of phenolic compounds. A total of 59 compounds were detected including 26 O- and C-glycosidated flavonoids and polyphenols, nine organic acids, seven amino acids, GABA, sucrose, glucose, myo-inositol, and five other non-identified compounds. Two of the identified compounds are the previously undescribed C-glycosyl flavonoids, apigenin-4'-O-β-glucopyranosyl, 8-C-β-(6″acetyl)-glucopyranoside and apigenin-4-O-β-glucopyranosyl-8-C-β-neohesperidoside. These C-glycosyl flavonoids were isolated to confirm their proposed structures by NMR and LCMS analysis. The PCA score plots obtained from the H NMR data of the studied Passiflora samples showed P. cumbalensis and P. uribei as the species with the most distinguishable chemical profile. In addition, a correlation analysis using OPLS-DA was conducted between H-NMR data and the quorum quenching activity (QQ) of Chromobacterium violaceum ATCC 31532. This analysis revealed P. lehmannii, and P. uribei extracts to be the most active, and apigenin-4'-O-β-glucopyranosyl, 8-C-β-(6″acetyl)-glucopyranoside and apigenin-4-O-β-glucopyranosyl-8-C-β-neohesperidoside were identified as possibly responsible for the QQ activity. To confirm this, QQ activity of both compounds was tested against C. violaceum ATCC 3153. An inhibition of violacein production of 0.135 mM (100 μg/mL) and 0.472 mM (300 μg/mL) was observed for apigenin-4'-O-β-glucopyranosyl,8-C-β-(6″acetyl)-glucopyranoside and apigenin-4-O-β-glucopyranosyl-8-C-β-neohesperidoside respectively, while bacterial growth was unaffected in both cases. Furthermore, both compounds showed the ability to inhibit the production of the toxoflavin of the phytopathogen Burkholderia glumae ATCC 33617.
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http://dx.doi.org/10.1016/j.phytochem.2020.112272DOI Listing
April 2020

Investigation of chemomarkers of astragali radix of different ages and geographical origin by NMR profiling.

Molecules 2015 Feb 17;20(2):3389-405. Epub 2015 Feb 17.

Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.

Astragalus roots from Astragalus membranaceus Bunge or Astragalus membranaceus var. mongholicus (Bunge) Hsiao are among the most popular traditional medicinal plants due to their diverse therapeutic uses based on their tonic, antinephritic, immunostimulant, hepatoprotectant, diuretic, antidiabetic, analgesic, expectorant and sedative properties. Currently, the herb is produced or cultivated in various sites, including 10 different locations in China with very diverse environmental conditions. These differences affect their metabolic pools and consequently their medicinal properties. The comparative metabolic profiling of plants of different geographical origins or ages could contribute to detect biomarkers for their quality control and thus guarantee the efficacy of the herbal medicines produced with this drug. In this paper nuclear magnetic resonance spectroscopy (NMR)-based metabolomics was applied for to plants of different origins and age for this purpose. The results of this study show that in the set of samples evaluated, age is more discriminating than geographical location. The quantity of individual flavonoids and some primary metabolites contributed most to this age differentiation. On the other hand, based on the analysis of orthogonal partial least square (OPLS) modeling, the marker metabolites for the geographical origin were saponins and isoflavonoids.
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http://dx.doi.org/10.3390/molecules20023389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272454PMC
February 2015