Publications by authors named "Emanuele Cozzani"

153 Publications

Considerations on SARS-Cov-2 vaccines in patients with autoimmune blistering diseases.

Eur J Dermatol 2021 Jun;31(3):415-417

Section of Dermatology, Department of Health Sciences (DISSAL), University of Genoa, Genoa, Italy, Dermatology Unit, San Martino Policlinic Hospital IRCCS, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1684/ejd.2021.4043DOI Listing
June 2021

Pruritic Erythematous Macules in a Patient with Systemic Sarcoidosis: A Quiz.

Acta Derm Venereol 2021 Jul 15. Epub 2021 Jul 15.

Department of Dermatology, Di.S.Sal., University of Genoa, San Martino Polyclinic Hospital IRCCS, Largo Rosanna Benzi 10, IT-16132 Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2340/00015555-3878DOI Listing
July 2021

Characterizing the proteome of bullous pemphigoid blister fluid utilizing tandem mass tag labeling coupled with LC-MS/MS.

Arch Dermatol Res 2021 Jun 21. Epub 2021 Jun 21.

Division of Dermatology, Rush University Medical Center, 707 S Wood St. Suite 220, Chicago, IL, USA.

Bullous pemphigoid is an autoimmune blistering disease caused by autoantibodies against components of the cutaneous basement membrane zone. Autoantibodies lead to complement-dependent and -independent inflammation and blistering. Blister fluid is a valuable biologic resource, as it provides insight into both systemic and local microenvironment responses. Here, we utilized liquid chromatography with tandem mass spectrometry to characterize the bullous pemphigoid blister fluid proteome. We then depleted exosomes to better understand the exosomal versus non-exosomal proteome. We identified 339 proteins in the blister fluid of bullous pemphigoid patients. Gene ontology demonstrated enrichment of several key biologic processes including innate immune response, neutrophil degranulation, platelet degranulation, and complement activation. Exosome depletion resulted in a significant decrease in normalized reporter intensities of 192 proteins, consistent with our observation of a large number of exosomal proteins found in the blister fluid. We then compared the bullous pemphigoid blister fluid proteome to prior proteomic datasets in suction blister fluid, snake bites, and thermal burns, identifying 76 proteins unique to bullous pemphigoid. These include major basic protein, eosinophil peroxidase, galectin-10, and the immunoglobulin epsilon heavy constant region, consistent with tissue eosinophilia. We lastly validated several previously reported blister fluid exosomal components. Blister fluid in bullous pemphigoid contains a mixture of numerous biologic processes. While many of these processes are shared with blistering from alternative causes, we have identified several notable features unique to bullous pemphigoid.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00403-021-02253-8DOI Listing
June 2021

Correlation Between Skin and Affected Organs in 52 Sclerodermic Patients Followed in a Diseases Management Team: Development of a Risk Prediction Model of Organ-Specific Complications.

Front Immunol 2021 2;12:588753. Epub 2021 Jun 2.

Dermatologic Unit, University of Genoa, DiSSal, Ospedale-Policlinico San Martino, IRCCS, Genova, Italy.

Objective: To identify the existence of a correlation among the various organs affected, focusing primarily on immuno-dermatological aspects, and to create a risk prediction model of organ-specific complications.

Material And Methods: Fifty-two patients with stable scleroderma, followed between 2015 and 2019, were investigated through an extensive multidisciplinary evaluation in the last year.

Results: Patients with lung involvement presented a worse degree of skin fibrosis than patients without it (p <0.001). No relationship was observed for the heart, kidney, and esophagus. Patients with pulmonary involvement had a lower pressure of the low esophagus sphincter and a higher Warrick score than patients without it (p <0.05). Age was significantly higher in patients with kidney involvement. Diffuse scleroderma patients had a worse pulmonary impairment than limited scleroderma patients (p <0.05). The manometric "sclerodermic" pattern was observed to be the most frequent (55.6%, p <0.05) in dcSSc patients while the sclerodermic and normal pattern were equally represented (41.2 and 32.4% respectively, p <0.05) in lcSSc patients. When compared to the negative serological groups, anti-Scl-70 positive patients presented a worse lung involvement while anti-centromere patients presented a better lung outcome (p <0.05). PM-Scl 100/75 positive patients presented mostly a pulmonary fibrotic pattern (p <0.05) and, also, heart complications were more likely associated with anti PM-Scl 100/75 positivity (p <0.05). The risk prediction model for organ-specific complications had an accuracy of 84.4% (95%CI 78, 89) in complication-site prediction, AUC of 0.871, 86% of sensitivity, and 83% of specificity, Cohen's Kappa (k) of 0.68.

Conclusions: Out of all the organs studied, the skin is the one that correlates with the lung. Patients with a diffuse form of disease presented more frequently the anti Scl-70 antibody and had a worse lung and esophageal involvement (scleroderma pattern) than the negative group. Conversely, patients with limited disease presented all positive for the anti-centromere antibody with a better lung involvement than the negative group, without any difference among the esophageal manometric pattern. Anti PM-Scl 100/75 antibody patients were associated with pulmonary fibrosis and presented cardiac involvement. The model created has demonstrated excellent values of sensitivity, specificity, and accuracy, but further studies are needed for validation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.588753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207468PMC
June 2021

Treatment of moderate-to-severe plaque psoriasis with tildrakizumab in the real-life setting.

Drugs Context 2021 21;10. Epub 2021 May 21.

Division of Dermatology, Department of Health and Science (DissaL), Policlinico San Martino Hospital, IRCCS, Genova, Italy.

Background: Tildrakizumab is a high-affinity, humanized IgG1κ monoclonal antibody targeting the p19 subunit of IL-23, which is a key regulatory cytokine in psoriasis. Based on evidence from clinical trials, tildrakizumab is approved for the treatment of moderate-to-severe plaque psoriasis in patients eligible for systemic therapy.

Methods: We report our clinical experience with 26 patients followed up to 24 weeks.

Results: No adverse event was observed and no patient discontinued tildrakizumab. Whilst no patient had a Psoriasis Area and Severity Index (PASI) score of <5 at baseline, at week 4, 8 (32%) patients had a PASI score of <3 and 6 of these had a PASI score of 0. At week 12, 19 (79%) patients had a PASI score of <5 and, among these, 18 had a PASI score of <3 of whom 16 had a PASI score of 0. At week 24, 22 (96%) patients had a PASI score of <3 and 20 (87%) had a PASI score of 0. Quality of life was improved after 4 weeks and through the whole period of observation.

Conclusion: Our experience in the real-life setting confirms the efficacy and safety of tildrakizumab as demonstrated by clinical trials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7573/dic.2021-2-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152773PMC
May 2021

Atypical and Rare Forms of Cutaneous Lupus Erythematosus: The Importance of the Diagnosis for the Best Management of Patients.

Dermatology 2021 Jun 3:1-10. Epub 2021 Jun 3.

Section of Dermatology, Department of Health Sciences, University of Genoa, Genoa, Italy.

Lupus erythematosus (LE) is an autoimmune disease with a wide range of clinical and cutaneous manifestations. Along with the well-known typical cutaneous manifestations of LE, some cutaneous manifestations are rarer, but still characteristic, enabling the dermatologist and the general practitioner who know them to suspect cutaneous LE (CLE) and investigate a possible underlying systemic involvement. Indeed, not infrequently a skin manifestation is the first presentation of systemic LE (SLE), and >75% of SLE patients show signs of skin disease during the course of the illness. Especially, SLE involvement occurs in cases of acute CLE, while it is uncommon in subacute CLE and rare in chronic CLE. This review aims to concentrate especially on atypical cutaneous manifestations of LE to enable the clinician to diagnose even the rarest forms of CLE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000515766DOI Listing
June 2021

Bulky Condyloma Acuminata following Ustekinumab Treatment for Plaque Psoriasis: A Case Report.

Case Rep Dermatol 2021 Jan-Apr;13(1):244-247. Epub 2021 Apr 21.

Section of Dermatology, DISSAL, San Martino-IST Polyclinic Hospital, University of Genoa, Genoa, Italy.

Ustekinumab is a fully human monoclonal antibody targeting the pro-inflammatory cytokines interleukin (IL)-12 and IL-23 approved for the treatment of psoriasis and psoriatic arthritis. We report a case of a patient treated for chronic plaque psoriasis with ustekinumab who developed bulky condyloma acuminata shortly after initiating the treatment. Although ustekinumab has already been described in literature associated with other forms of human papilloma virus skin infections, this is the first case to our knowledge in which ustekinumab is associated with genital warts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000509178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138217PMC
April 2021

Giant nevus comedonicus of the entire lower left limb protecting from the associated bullous pemphigoid: A hypothetical role of NEK9 mutation?

J Dermatol 2021 May 17. Epub 2021 May 17.

Section of Dermatology, DiSSal, San Martino Polyclinic Hospital IRCCS, University of Genova, Genova, Italy.

We present the case of a 92-year-old woman, with bullous pemphigoid (BP) and a concomitant nevus comedonicus (NC) presenting as an asymptomatic, linear lesion of the entire lower left limb, formed by multiple comedones. Dermoscopy of the NC and histopathology confirmed the clinical and dermoscopic suspect of giant congenital nevus comedonicus. The two entities presented no overlap. In this article, we speculate that a mutation of the NEK9 gene, associated with NC, probably altering the normal follicular differentiation in NC lesions, may hypothetically also influence the expression of BPAG2 in NC. This might possibly influence a protective role of NC lesions towards BP. Undoubtedly, genetic studies would be needed to confirm or reject the proposed hypothesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1346-8138.15879DOI Listing
May 2021

A case of pseudochromhidrosis in a whole family during COVID-19 lockdown.

Ital J Dermatol Venerol 2021 Apr;156(2):249-250

Unit of Dermatology, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S2784-8671.21.06933-9DOI Listing
April 2021

COVID-19 "second wave" and vaccines: the dermatologists' perspective.

Int J Dermatol 2021 Jul 25;60(7):889-890. Epub 2021 Mar 25.

Di.S.Sal. Section of Dermatology, University of Genoa, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/ijd.15547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251220PMC
July 2021

Considerations on inhibition of IL-23 in psoriatic women of childbearing potential.

Dermatol Ther 2021 05 17;34(3):e14931. Epub 2021 Mar 17.

Di.S.Sal. Section of Dermatology, University of Genoa, Genoa, Italy.

Lack of safety data on pregnant women determines difficulty in choosing the correct biologic agent to treat psoriasis in women of childbearing potential. Studies have postulated a role of IL-23 in unexplained recurrent spontaneous abortions. This gives rise to consideration about use of anti-IL-23 drugs in treatment of psoriasis in women of childbearing potential.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dth.14931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244100PMC
May 2021

Spastic quadriplegia following intradermal use of hydrogen peroxide in the tardive curettage procedure for the treatment of a giant congenital nevus.

G Ital Dermatol Venereol 2020 Dec;155(6):780-782

Department of Legal Medicine, IRCCS San Martino University Hospital, Genoa, Italy.

The possible transformation of a giant congenital melanocytic nevi (GCMN) in malignant melanoma estimated from 0.05% to 40% depend on the size of the lesions. Many are the surgical procedures proposed, including: full or partial-thickness excisions, dermabrasion, curettage in the first weeks of life and laser treatment. The curettage technique has been proposed in the literature for the treatment of GCMN in the first few weeks of life and defined as a relatively atraumatic surgery procedure without general complications. The authors report the first case in the literature of embolization due to use of subcutaneous peroxide infiltration before a tardive curettage procedure in a newborn case of GCMN resulting in spastic quadriplegia with dystonic reaction. Consequently, a lawsuit, due to this medical malpractice, has been opened.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0392-0488.16.05231-7DOI Listing
December 2020

Managing Telemedicine for Wounds in the COVID-19 Era: A New Proposal.

Adv Skin Wound Care 2021 03;34(3):120

University of Genoa, San Martino Polyclinic Hospital IRCCS, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/01.ASW.0000733208.81005.ddDOI Listing
March 2021

Neoadjuvant imatinib as treatment preceding surgery for vulvar dermatofibrosarcoma protuberans.

Dermatol Ther 2021 03 20;34(2):e14860. Epub 2021 Feb 20.

Di.S.Sal. Section of Dermatology, University of Genoa, San Martino Polyclinic Hospital IRCCS, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dth.14860DOI Listing
March 2021

Multiple rubbery nodules on the scalp.

JAAD Case Rep 2021 Feb 5;8:80-82. Epub 2021 Jan 5.

DiSSal Section of Dermatology, San Martino Polyclinic Hospital IRCCS, University of Genoa, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdcr.2020.12.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829102PMC
February 2021

Italian expert-based recommendations on the use of photo(chemo)therapy in the management of mycosis fungoides: Results of an e-Delphi consensus.

Photodermatol Photoimmunol Photomed 2021 Jul 1;37(4):334-342. Epub 2021 Feb 1.

SOD Clinica di Dermatologia, Azienza Ospedaliero Universitaria Ospedali Riuniti di Ancona, Ancona, Italy.

Background: Phototherapy is a mainstay for the treatment of MF. However, there is scarce evidence for its use, mostly due to the lack of a unified schedule.

Aims: The primary aim of this study was to establish the first structured, expert-based consensus regarding the indications and technical schedules of NB-UVB and PUVA for MF. The secondary aim was to determine the consensus level for each specific item.

Materials & Methods: E-delphi study. Item-specific expert consensus was defined as the number of "Totally Agree" results to ≥80% of the panelists. Cronbach alpha index ≥0.7 was used as a measure of homogeneity in the responses among questions related to the same topic.

Results: Overall, there was a high homogeneity among responders (0.78). On specific topics, the highest grade was observed for technical items (0.8) followed by indications for early (0.73) and advanced stages (0.7).

Conclusions: Items related to the most canonical indications of phototherapy and to treatment schedules showed the highest agreements rates. There is consensus about the use of standardized treatment schedules for the induction and consolidation phases for NB-UVB and PUVA in MF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/phpp.12658DOI Listing
July 2021

Is ultraviolet radiation avoidance affecting bone health in melanoma patients?

Photodermatol Photoimmunol Photomed 2021 Jul 28;37(4):329-333. Epub 2021 Jan 28.

Section of Dermatology, Department of Health Sciences (DISSAL), University of Genoa, Genoa, Italy.

Background: Ultraviolet (UV) radiation has numerous beneficial effects on human health, including stimulating vitamin D and serotonin production and immuno-regulatory activities. Conversely, UV radiation is also classified as a group one carcinogen by the International Agency for Research on Cancer.

Purpose: To investigated the effects of UV radiation avoidance in melanoma patients in terms of vitamin D levels but also of bone mineral density and trabecular bone microarchitecture.

Methods: We conducted an observational study investigating the effects of UV radiation avoidance in 31 melanoma patients in terms of vitamin D levels but also of bone mineral density and trabecular bone microarchitecture by using dual-energy X-ray absorptiometry scan. Data were compared with two control groups of healthy subjects, who were chronically exposed or not exposed to UV radiation during their lifetime.

Results: Melanoma patients had on average slightly lower levels of vitamin D, without reaching statistical significance (P = .135). No significant difference was found across the three groups on T-scores of femoral neck (P = .544), of total hip (P = .617) and of lumbar spine P = .155). No significant difference was found on and trabecular bone score across exposure groups (P = .895).

Conclusion: UV radiation avoidance does not seem to significantly impact vitamin D levels nor bone health in melanoma patients. Thus, UV protective behavior is advisable for all melanoma patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/phpp.12657DOI Listing
July 2021

Lichen planus pigmentosus inversus concomitant with two internal malignancies. A paraneoplastic cutaneous sign?

G Ital Dermatol Venereol 2021 Jan 11. Epub 2021 Jan 11.

Di.S.SAL Section of Dermatology, University of Genoa, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0392-0488.20.06851-0DOI Listing
January 2021

Can Methotrexate be a successful treatment for unresponsive generalized annular elastolytic giant cell granuloma? Case report and review of the literature.

Dermatol Ther 2021 01 30;34(1):e14705. Epub 2020 Dec 30.

DISSAL Department of Dermatology, University of Genova Ospedale Policlinico San Martino IRCCS, Genoa, Italy.

Annular elastolytic giant cell granuloma (AEGCG) is a rare granulomatous skin disorder, characterized by erythematous plaques with elevated borders and hypopigmented center, occurring mainly on sun exposed-skin. Histologically it presents with elastophagocytosis and elastolysis. There is no established first line treatment for AEGCG, especially for the generalized form. In a small number of cases, antimalarial drugs and tranilast, associated to topical or oral steroids, have been proposed to treat generalized AEGCG with partial benefits. We herein present the case of a patient with AEGCG aged 74 years, who was unresponsive to classical therapies, and then successfully treated with methotrexate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dth.14705DOI Listing
January 2021

Six months into the pandemic. A review of skin manifestations in SARS-CoV-2 infection.

Dermatol Ther 2021 Jan 14;34(1):e14641. Epub 2020 Dec 14.

Department of Dermatology, University of Genoa, Genoa, Italy.

During the COVID-19 pandemic, various cutaneous manifestations have been described as associated with SARS-CoV2 infection. It is debated if skin lesions could represent a diagnostic or prognostic indicator. Specifically, it is unclear whether skin lesions may be used to perform an early diagnosis and/or to predict worse outcomes. In this review, we described the cutaneous signs so far reported as COVID-19-related and discussed their incidence, clinico-pathological features, and diagnostic and prognostic value.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dth.14641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883133PMC
January 2021

Unilateral nevoid telangiectasia: a rare and underdiagnosed skin disease.

Eur J Dermatol 2020 Oct;30(5):601-602

Department of Dermatology, Di.S.Sal., University of Genoa, San Martino Polyclinic Hospital IRCCS, Largo Rosanna Benzi 10, 16132 Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1684/ejd.2020.3867DOI Listing
October 2020

H Syndrome retrospectively diagnosed: The importance of recognizing cutaneous signs.

Clin Case Rep 2020 Oct 14;8(10):2046-2048. Epub 2020 Jul 14.

DiSSal Section of Dermatology San Martino Polyclinic Hospital University of Genoa Genoa Italy.

We present a case of a retrospectively diagnosed H syndrome in a man who died of a probable heart infarction. We highlight the importance of recognizing cutaneous hallmarks of this syndrome for better clinical management and prevention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ccr3.3082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7562838PMC
October 2020

The HLA-Cw6 Dilemma: Is It Really an Outcome Predictor in Psoriasis Patients under Biologic Therapy? A Monocentric Retrospective Analysis.

J Clin Med 2020 Sep 28;9(10). Epub 2020 Sep 28.

Di.S.Sal. Section of Dermatology, University of Genoa, Via Pastore 1, 16132 Genoa, Italy.

HLA-Cw6 is one of the most strongly associated psoriasis susceptibility alleles. Data regarding correlation between HLA-Cw6 status and biologic treatment outcomes are divergent. The aim of our study in our cohort of psoriatic patients was to explore if the HLA-Cw6 status influences the response rate to biologic therapies at 16 and 48 weeks. One hundred and one psoriatic patients eligible for biologic therapies were enrolled. HLA-C*06 alleles were detected from their blood samples. The effectiveness of antipsoriatic treatments was reported as 90% Psoriasis Area and Severity Index reduction (PASI90). All biologics showed efficacy at week 16, without significant differences between one another. HLA-Cw6 status did not seem to affect baseline characteristics, or treatment response at week 16. At week 48, IL-12/23 and IL-17 targeting drugs were more effective on Cw6-positive patients than on Cw6-negative patients. Conversely, TNF-targeting drugs seemed to be more effective on Cw6- negative patients than on Cw6-positive patients. The HLA-Cw6 test could well deserve to be integrated into the clinical laboratory work-up supporting the choice of the correct biologic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm9103140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600180PMC
September 2020

Soft, Erythematous Nodule of the Ankle with a String of Pearls Vascular Pattern: A Quiz.

Acta Derm Venereol 2020 Oct 6;100(17):adv00288. Epub 2020 Oct 6.

Section of Dermatology, Department of Health Sciences (Di.S.Sal.), University of Genoa, IT-16132 Genoa, Italy. E-mail:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2340/00015555-3651DOI Listing
October 2020

Annular and arciform lesions of the palms as unique manifestations of secondary syphilis.

Int J STD AIDS 2020 11 27;31(13):1323-1326. Epub 2020 Sep 27.

Dermatology Unit, Ospedale Policlinico San Martino, Genoa, Italy.

We describe a patient presenting with annular-arciform symmetric lesions of the palms as the unique manifestation of secondary syphilis. The polymorphism of skin lesions in secondary syphilis depends entirely on the degree of inflammatory infiltrate, the level of vascular involvement and the resulting ischemia of the skin. Besides the transient and diffuse maculo-papules (roseola syphilitica), secondary syphilis skin lesions may have different morphology and shape. It has already been reported that in cases of reinfections by the skin lesions may be arciform, as in our patient, and asymmetric. Indeed, previous syphilis may attenuate the clinical and laboratory manifestations of a new infection with , that occurs with less severe skin manifestations or presents as latent syphilis. However, to our knowledge, annular-arciform lesions of the palms as unique manifestations of secondary syphilis have never been described. Clinicians should be well trained to recognize the atypical presentations of syphilis, especially in patients who have already received a syphilis diagnosis, since its incidence is steadily increasing in Europe, especially in men having sex with men.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0956462420948354DOI Listing
November 2020

Comedonal variant of chronic cutaneous lupus erythematosus causing mutilation of the earlobe.

JAAD Case Rep 2020 Sep 11;6(9):843-844. Epub 2020 Jun 11.

University of Genoa, DiSSal Section of Dermatology, San Martino Polyclinic Hospital IRCCS, Genoa, Italy.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdcr.2020.06.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452277PMC
September 2020

Spontaneous Ecchymotic Lesions with Systemic Symptoms: A Quiz.

Acta Derm Venereol 2020 Aug 18;100(15):adv00246. Epub 2020 Aug 18.

DiSSal Section of Dermatology, University of Genoa, IRCCS San Martino Policlinic Hospital, IT-16132 Genoa, Italy.

is missing (Quiz).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2340/00015555-3606DOI Listing
August 2020

Apremilast interferes with the TGFβ1-induced transition of human skin fibroblasts into profibrotic myofibroblasts: in vitro study.

Rheumatology (Oxford) 2020 12;59(12):3927-3938

Research Laboratory and Academic Division of Clinical Rheumatology, Department of Internal Medicine, University of Genova, IRCCS Polyclinic San Martino Hospital, Genoa.

Objectives: Fibroblast-to-myofibroblast transition and extracellular matrix overproduction represent progressive events in chronic inflammatory and fibrotic diseases, in which TGFβ1 is one of the key mediators. Phosphodiesterase 4 (PDE4) acts as a proinflammatory enzyme through the degradation of cyclic adenosine monophosphate and it is overexpressed in skin fibroblasts. The study investigated how apremilast (a PDE4 inhibitor) interferes with the intracellular signalling pathways responsible for the TGFβ1-induced fibroblast-to-myofibroblast transition and profibrotic extracellular matrix protein synthesis.

Methods: Cultured human skin fibroblasts were stimulated with TGFβ1 (10 ng/ml) alone or combined with apremilast (1 and 10 μM) for 4, 16 and 24 h. Other aliquots of the same cells were previously stimulated with TGFβ1 and then treated with apremilast (1 and 10 μM) for 4, 16 and 24 h, always under stimulation with TGFβ1. Gene and protein expression of αSMA, type I collagen (COL1) and fibronectin were evaluated, together with the activation of small mothers against decapentaplegic 2 and 3 (Smad2/3) and extracellular signal-regulated kinase (Erk1/2) proteins.

Results: Apremilast reduced the TGFβ1-induced increase in αSMA, COL1 and fibronectin gene expression at 4 and 16 h, and protein synthesis at 24 h of treatment in cultured fibroblasts, even for cells already differentiated into myofibroblasts by way of a previous stimulation with TGFβ1. Apremilast inhibited the TGFβ1-induced Smad2/3 and Erk1/2 phosphorylation at 15 and 30 min.

Conclusion: Apremilast seems to inhibit in vitro the fibroblast-to-myofibroblast transition and the profibrotic activity induced by TGFβ1 in cultured human skin fibroblasts by downregulating Smad2/3 and Erk1/2 intracellular signalling pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/rheumatology/keaa249DOI Listing
December 2020

Coronavirus disease 2019 (COVID 19) and Malaria: Have anti glycoprotein antibodies a role?

Med Hypotheses 2020 10 25;143:110036. Epub 2020 Jun 25.

Dermatologic Unit, University of Genoa, DiSSal, Ospedale-Policlinico San Martino, IRCCS, Largo R. Benzi 10, 16132 Genova, Italy. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mehy.2020.110036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314692PMC
October 2020

P53 staining index and zonal staining patterns in actinic keratoses.

Arch Dermatol Res 2021 May 8;313(4):275-279. Epub 2020 Jul 8.

Dermatology Unit, Department of Health Sciences (DISSAL), University of Genoa, Genoa, Italy.

Actinic keratoses (AKs) are common dysplastic lesions resulting from chronic excessive ultraviolet exposure. Neither the clinical grade of thickness nor the histological grade of dysplasia seems valid predictors of aggressive potential of AKs. Instead, the mutational status in AKs appears to predict well the clinical course. TP53 gene mutations result in a non-functional protein resistant to degradation, thus immunohistochemical staining for p53 can suggest mutation status. Increased p53 was associated with progression from AK to squamous cell carcinoma. To investigate how the intensity of p53 staining (p53 staining index) varies according to body site, histological subtype and grade dysplasia of AKs. Secondly, we sought to investigate the distribution in the epidermal layers of non-functional p53 (zonal staining patterns). p53 staining index was greater than 50% in 90.7% of AKs. p53 staining index was significantly higher in older age (p < 0.0093) and in facial AKs compared to other body areas (p = 0.03). A significant correlation between p53 staining index and grade of dysplasia was observed (p = 0.006) and between p53 staining index and zonal p53 staining pattern (p = 0.003). No significant differences in p53 staining index among the various histological AK types were observed. No correlation between clinical and histological grade. All AKs, independently from their clinical appearance, should be treated but special attention is required for AKs on severely photodamaged skin on the face and in older patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00403-020-02104-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043885PMC
May 2021
-->