Publications by authors named "Emanuel Celice Castilho"

8 Publications

  • Page 1 of 1

Follicular Lesions with Papillary Nuclear Characteristics: Differences in Chromatin Detected by Computerized Image Analysis.

Arch Endocrinol Metab 2021 May;64(5):630-635

Departamento de Medicina Interna, Faculdade de Medicina de Botucatu, Universidade Estadual de São Paulo (Unesp), Botucatu, SP, Brasil,

Objective: Follicular lesions of the thyroid with papillary carcinoma nuclear characteristics are classified as infiltrative follicular variant of papillary thyroid carcinoma-FVPTC (IFVPTC), encapsulated/well demarcated FVPTC with tumour capsular invasion (IEFVPTC), and the newly described category "non-invasive follicular thyroid neoplasm with papillary-like nuclear features" (NIFTP) formerly known as non-invasive encapsulated FVPTC. This study evaluated whether computerized image analysis can detect nuclear differences between these three tumour subtypes.

Methods: Slides with histological material from 15 cases of NIFTP and 33 cases of FVPTC subtypes (22 IEFVPTC, and 11 IFVPTC) were analyzed using the image processing program. Tumour cells were compared for both nuclear morphometry and chromatin textural characteristics.

Results: Nuclei from NIFTP and IFVPTC tumours differed in terms of chromatin textural features (grey intensity): mean (92.37 ± 21.01 72.99 ± 14.73, p = 0.02), median (84.93 ± 21.17 65.18 ± 17.08, p = 0.02), standard deviation (47.77 ± 9.55 39.39 ± 7.18; p = 0.02), and coefficient of variation of standard deviation (19.96 ± 4.01 24.75 ± 3.31; p = 0.003). No differences were found in relation to IEFVPTC.

Conclusion: Computerized image analysis revealed differences in nuclear texture between NIFTP and IFVPTC, but not for IEFVPTC.
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May 2021

Thyroglobulin levels and thyroglobulin/thyrotropin ratio could predict the success of the ablative/therapeutic I in the differentiated thyroid cancers.

Endocr Res 2017 02 4;42(1):42-48. Epub 2016 May 4.

a Internal Medicine Department , Botucatu Medical School, São Paulo State University , Unesp, Botucatu , SP , Brazil.

Background: Stimulated thyroglobulin (STg) levels in patients with differentiated thyroid carcinomas (DTCs) after total thyroidectomy (TT) and before radioactive iodine (I) ablation/therapy (RIT) are predictive of therapeutic success but can be influenced by the thyroid-stimulating hormone (TSH) level.

Objectives: This study compared the reliability of the STg/TSH ratio and STg measurement in predicting the success of RIT.

Methods: Sixty-three DTC patients submitted to TT were assessed retrospectively to compare the ability of STg level and the STg/TSH ratio to predict successful RIT.

Results: In this study 48 (76.2%) patients had successful RIT. The successful and unsuccessful groups received different I doses and had different STg levels and STg/TSH ratios. The STg and STg/TSH ratio cutoff values that predicted successful RIT were 4.41 ng/mL (sensitivity of 86.7% and specificity of 77%) and 0.093 (sensitivity of 80% and specificity of 79.2%), respectively. Age, STg level, STg/TSH ratio, and I dose were associated with successful RIT, but after multivariate analysis only STg remained associated (p < 0.05).

Conclusion: In conclusion, our data suggest that the STg/TSH ratio and measurement of STg are equally reliable in predicting successful RIT in DTC patients.
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February 2017

Pharyngoesophageal diverticulum: evaluation of treatment results.

Rev Col Bras Cir 2013 Mar-Apr;40(2):104-9

Department of Surgery and Orthopedics, Medical School of Botucatu-Paulista State University, Botucatu, São Paulo State, Brazil.

Objective: To evaluate the postoperative outcome of patients with pharyngoesophageal diverticulum submitted to surgical and endoscopic treatments.

Methods: We retrospectively analyzed 36 patients with pharyngo-esophageal diverticulum treated at the Hospital of the Medical School of Botucatu - UNESP. Patients were divided into two groups, depending on the treatment: group 1 (n = 24): diverticulectomy associated myotomy through a left cervicotomy; group 2 (n = 12): endoscopic diverticulostomy with linear stapler.

Results: Operative mortality was zero in both groups. Early complications: group 1 - two patients developed cervical fistula and two, hoarseness; group 2 - none. Late complications: group 1 - none; group 2: recurrence of dysphagia in four patients (p = .01). Mean follow-up was 33 months for group 1 and 28 months for group 2.

Conclusion: Both procedures were effective in remission of dysphagia. Surgical treatment showed superiority to endoscopy, with resolution of dysphagia with a single procedure. Endoscopic treatment should be reserved for the elderly and those with comorbidities.
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April 2014

Comparative study of the behavior of p53 immunoexpression in smoking associated lesions: Reinke's edema and laryngeal carcinoma.

Inhal Toxicol 2013 Jan;25(1):17-20

Botucatu Medical School, UNESP, Sao Paulo, Brazil.

Objective: To assess the behavior of the immunoexpression of protein p53 in Reinke's edema and laryngeal squamous cell carcinoma.

Study Design: retrospective.

Methods: we recovered the histological paraffin blocks of patients who were subjected to Reinke's edema and laryngeal squamous cell carcinoma surgery in 2000-2011. The paraffin blocks were cut into 3-µm sections; the specimens were prepared in silanized slides (one slide for each paraffin block) and subjected to immunohistochemical reaction according to the Avidin Biotin Peroxidase method. Monoclonal primary anti-p53 antibodies were used at 1:50 dilution. Slides were examined under a light microscope at different magnitudes and results were interpreted based on the degree of brown staining in the nuclei of epithelial cells and in the extent of the fragment by using a semi-quantitative score from 0 to 3.

Results: 67 slides of Reinke's edema and 60 slides of laryngeal squamous cell carcinoma were included. Scores 2 and 3 for staining of the nuclei of epithelial cells were recorded for 46 slides of Reinke's edema (68.65%) and for 57 slides of laryngeal squamous cell carcinoma (95%). As to the extent of the fragment, scores 2 and 3 were recorded for 74% slides of Reinke's edema and for 95% slides of carcinomas.

Conclusion: the positive immunoexpression for protein p53, positive in 95% carcinomas and 74% Reinke's edemas, makes us aware of the possible preneoplastic condition of the latter lesion. Further studies are needed to identify and reveal the genetic changes that lead to these results.
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January 2013

Second primary tumors in patients with head and neck cancer.

Curr Oncol Rep 2011 Apr;13(2):132-7

Department of Medicine, Taubaté University, Av. Tiradentes, 241, Taubaté, Brazil.

This is a review on second primary tumors in patients with head and neck cancer. These patients have a high risk of developing other cancers simultaneously or subsequently. The incidence of multiple primary tumors in this population can be as high as 27%. Recurrences are the most common cause of treatment failure within the first 2 years of follow-up. After the third year the diagnosis of a second primary tumor becomes the most important cause of morbimortality in head and neck cancer patients, especially in those treated for cancers early diagnosed. Most second primary tumors occur in the upper aerodigestive tract (40%-59%), lung (31%-37.5%), and esophagus (9%-44%). Patients who develop second primary tumor have a significant reduction of survival expectancy.
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April 2011

Retropharyngeal abscess and stridor in infants.

Braz J Otorhinolaryngol 2009 Mar-Apr;75(2):319

Disciplina de Otorrinolaringologia, Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço da Faculdade de Medicina de Botucatu, Distrito de Rubião Junior, Botucatu SP 18618-970.

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April 2010

[Hoarseness after tracheal intubation.].

Rev Bras Anestesiol 2006 Apr;56(2):189-99


Background And Objectives: To describe the main causes of hoarseness after undergoing tracheal intubation.

Contents: Hoarseness is one of the most common postoperatory symptoms after tracheal intubation and the effects vary in terms of duration, depending on the factors that caused it and on how severe the damage to the laryngeal structures. This study performed a brief check-up of the anatomical structures of the larynx, describing the main traumatic lesions in the region following tracheal intubation. It also emphasized the importance of caring for the larynx, as well as undergoing early diagnosis and treatment.

Conclusions: Traumatic lesions of the laryngeal structures that occur during intubation are the most common causes of hoarseness. As such, it is important to perform an early diagnosis and adopt preventive measures.
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April 2006

[Effects of tracheal tube cuff limit pressure (25 cmH2O) and "seal" pressure on tracheal mucosa of dogs.].

Rev Bras Anestesiol 2003 Dec;53(6):743-55

Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, FMB, UNESP.

Background And Objectives: Injuries of tracheal mucosa in contact with tracheal tube cuff is a function of cuff pressure and exposure time. This study aimed at analyzing injuries of tracheal mucosa in contact with tracheal tube cuff inflated to reach "seal" pressure or limit 25 cmH2O pressure, below critical 30 cmH2O, to prevent tracheal damage.

Methods: This study involved 16 dogs submitted to intravenous anesthesia and artificial ventilation. Dogs were randomly distributed into two experimental groups according to tracheal tube cuff pressure (Portex Blue Line, UK): Gseal (n = 8) cuff with minimum "seal" pressure to prevent air leakage during artificial ventilation; G25 (n=8) cuff inflated to 25 cmH2O. Cuff pressure was measured with a digital manometer at the beginning of the experiment (control) and 60, 120 and 180 minutes later. Animals were sacrificed and tracheal mucosa areas adjacent to the tracheal tube cuff were biopsed by scanning electronic microscopy (SEM).

Results: Mean cuff pressure was maintained between 24.8 and 25 cmH2O in G25 and between 11.9 and 12.5 cmH2O in Gseal. SEM changes were mild and not significantly different between groups (p > 0.30), with more severe injuries to tracheal areas in contact with the cuff as compared to areas adjacent or not to tracheal tube (p < 0.05).

Conclusions: In dogs under our experimental conditions, tracheal tube cuff inflation to 25 cmH2O limit or to "seal" pressure to prevent air leakage has determined minor injuries to the tracheal mucosa in contact with tracheal tube cuff, without significant differences between groups.
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December 2003