Publications by authors named "Emad Yahaghi"

53 Publications

Investigation of clinicopathological parameters in emergency colorectal cancer surgery: a study of 67 patients.

Arch Med Sci 2017 Oct 20;13(6):1394-1398. Epub 2016 Jul 20.

Department of Emergency Medicine, Alborz University of Medical Sciences, Karaj, Iran.

Introduction: The aim of the present study was to establish, having adjusted for case mix, the size of the differences in postoperative mortality and 5-year survival between patients presenting as an emergency with evidence of obstruction and perforation and the association of clinicopathological factors with mortality (bivariate analyses).

Material And Methods: The study included 67 patients who presented with colorectal cancer (CRC) between 2009 and 2013 in Iran. The mean age of the patients was 59.7 years. Of the 67 patients, 37 (55.22%) were male and 30 (44.77%) were female. Certain parameters that correlated with CRC and surgical treatment were investigated.

Results: Our results showed that 46 (68.65%) patients had obstruction, while perforation was observed in 21 (31.34%) cases. Among the patients with obstruction, obstruction of the right colon was observed in 29 (43.28%) cases. There was no significant difference in mortality rate between right and left colonic obstruction. Based on the bivariate analyses, our findings showed that death of patients was significantly related to tumor grade ( = 0.02) and TNM staging ( = 0.026), but no association was found between other parameters and death, including age, sex, and tumor site.

Conclusions: Compared with patients who undergo elective surgery for colon cancer, those who present as an emergency with evidence of obstruction or perforation have higher postoperative mortality rates and poorer cancer-specific survival. Also, colorectal cancer patients with emergency surgery showed aggressive histopathology and an advanced stage.
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http://dx.doi.org/10.5114/aoms.2016.61385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701685PMC
October 2017

Down-regulation of microRNA-182 and microRNA-183 predicts progression of osteosarcoma.

Arch Med Sci 2017 Oct 20;13(6):1352-1356. Epub 2016 May 20.

Department of Orthopedic Surgery, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Introduction: The aim of this study was to investigate the expression levels of microRNA-182 and microRNA-183 and their association with clinicopathological features in patients with osteosarcoma.

Material And Methods: Total RNA was purified from samples and noncancerous bone tissues and then quantitative real-time polymerase chain reaction was applied to evaluate the expression levels of microRNAs, and their relationship with clinicopathological features and survival in osteosarcoma patients.

Results: Our findings showed that expression of MiR-182 was clearly lower in osteosarcoma bone tissue (mean ± SD: 2.84 ±.07) compared with noncancerous bone tissues (6.23 ±1.72, = 0.004). On the other hand, lower expression of MiR-183 was seen in osteosarcoma bone tissue (1.43 ±0.59) when compared with normal tissues (4.36 ±2.47, = 0.036). Decreased expression of MiR-182 was clearly correlated with advanced clinical stage ( = 0.001), metastasis or recurrence ( = 0.024), and large tumor size ( = 0.032). Decreased expression of MiR-183 was associated with advanced TNM stage ( = 0.004), and metastasis or recurrence ( = 0.002). A multivariate Cox proportional hazards model revealed that low expression of MiR-182 and MiR-183 ( = 0.02; = 0.016), TNM stage ( = 0.04), and metastasis or recurrence ( = 0.03) were significantly associated with poor survival as independent prognostic factors.

Conclusions: These findings suggest that MiR-182 and MiR-183 may be associated with progression and metastasis of osteosarcoma.
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http://dx.doi.org/10.5114/aoms.2016.60091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701680PMC
October 2017

Diagnostic investigations of PLA2G16 and CDH11 expression levels as independent prognostic markers of human osteosarcoma.

Arch Med Sci 2017 Oct 5;13(6):1347-1351. Epub 2016 May 5.

Department of Orthopedic and Traumatology, Universitätsklinikum Bonn, Bonn, Germany.

Introduction: The aim of this study was to facilitate and deepen the understanding of the associations of the clinical significance of PLA2G16 and CDH11 in patients with osteosarcoma.

Material And Methods: We collected 50 paired osteosarcoma tissues and adjacent normal bone tissues and evaluated the expression of PLA2G16 and CDH11 by quantitavise reverse transcriptase real-time polymerase chain reaction.

Results: PLA2G16 expression was upregulated in osteosarcoma tissues when compared with adjacent normal bone tissues, and the difference was statistically significant (4.78 ±0.70 vs. 1.31 ±0.65; < 0.05). Our data indicated that high expression of PLA2G16 was significantly related to advanced TNM stage and metastasis or recurrence ( < 0.05). The expression level of CDH11 was lower in osteosarcoma tissues (median relative expression level ± SD: 6.29 ±1.43) than adjacent normal bone tissues (mean ± SD: 13.72 ±3.08, < 0.05). Our findings demonstrated that decreased expression of CDH11 was strongly linked to advanced TNM stage, and metastasis or recurrence ( < 0.05). Log-rank analysis showed that patients with high expression of PLA2G16 have shorter overall survival than those with low expression. Moreover, shorter overall survival was significantly correlated with decreased expression of CDH11. Multivariate Cox proportional hazards analysis showed that PLA2G16 ( = 0.028; HR = 2.621; 95% CI) and CDH11 ( = 0.023; HR = 2.81; 95% CI) expression and also metastasis or recurrence ( = 0.03; HR = 2.531; 95% CI) were independent prognostic factors for poor overall survival of osteosarcoma patients.

Conclusions: These findings suggest that PLA2G16 and CDH11 expression can be independent prognostic factors for poor overall survival of patients with osteosarcoma.
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http://dx.doi.org/10.5114/aoms.2016.59710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5701678PMC
October 2017

Retraction Note: Down-regulation of miR-133a and miR-539 are associated with unfavorable prognosis in patients suffering from osteosarcoma.

Cancer Cell Int 2016 4;16:84. Epub 2016 Nov 4.

Cellular & Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.

[This retracts the article DOI: 10.1186/s12935-015-0237-6.].
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http://dx.doi.org/10.1186/s12935-016-0359-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096315PMC
November 2016

Retraction Note to: Downregulation of miR-148b as biomarker for early detection of hepatocellular carcinoma and may serve as a prognostic marker.

Tumour Biol 2016 Nov 5. Epub 2016 Nov 5.

Department of Emergency Medicine, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

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http://dx.doi.org/10.1007/s13277-016-5477-0DOI Listing
November 2016

Retraction Note to: Evaluation of mRNA expression levels of IL-17A and IL-10 cytokines in cervical cancer.

Tumour Biol 2016 11 5. Epub 2016 Nov 5.

Department of Gynecology, Khanevadeh Hospital, AJA University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1007/s13277-016-5486-zDOI Listing
November 2016

Comparison of Manual Refraction Versus Autorefraction in 60 Diabetic Retinopathy Patients.

Med Arch 2016 Jul;70(4):280-283

Department of Ophthalmology, AJA University of Medical Sciences, Tehran, Iran.

Aim: The purpose of the study was to evaluate the comparison of manual refraction versus autorefraction in diabetic retinopathy patients.

Material And Methods: The study was conducted at the Be'sat Army Hospital from 2013-2015. In the present study differences between two common refractometry methods (manual refractometry and Auto refractometry) in diagnosis and follow up of retinopathy in patients affected with diabetes is investigated.

Results: Our results showed that there is a significant difference in visual acuity score of patients between manual and auto refractometry. Despite this fact, spherical equivalent scores of two methods of refractometry did not show a significant statistical difference in the patients.

Conclusion: Although use of manual refraction is comparable with autorefraction in evaluating spherical equivalent scores in diabetic patients affected with retinopathy, but in the case of visual acuity results from these two methods are not comparable.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5034982PMC
http://dx.doi.org/10.5455/medarh.2016.70.280-283DOI Listing
July 2016

The role and expression of miR-100 and miR-203 profile as prognostic markers in epithelial ovarian cancer.

Am J Transl Res 2016 15;8(5):2403-10. Epub 2016 May 15.

Department of Gynecology, Khanevadeh Hospital, AJA University of Medical Sciences Tehran, Iran.

Purpose: The present study was aimed to evaluate the clinical significance of miR-100 and miR-203 in epithelial ovarian cancer (EOC) patients.

Methods: The expression levels of miR-100/203 in EOC tissue and adjacent non-cancerous samples were determined by real-time RT-PCR. Associations between miRNAs expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier and statistically analyzed with the log-rank method between groups. Survival data were evaluated through multivariate. Cox regression analysis.

Findings: Our findings showed that miR-100 was significantly down-regulated in EOC tissue specimens than in adjacent non-cancerous tissues. The expression level of miR-203 was significantly higher in EOC tissues compared to adjacent non-cancerous tissues. Decreased expression of miR-100 was strongly associated with high FIGO stage (P=0.012). The high expression of miR-203 was significantly correlated with advanced FIGO stage (p=0.006), advanced histological grade (p=0.03). Kaplan-Meier analysis and log-rank test have suggested that EOC patients with down-regulated miR-100 expression and up-regulated miR-203 expression have shorter overall survival when compared with patients with other expression groups (log-rank test P<0.001). Multivariate Cox proportional hazards model indicated that the status of miR-100 and miR-203 expression levels were independent predictor of overall survival in patients with EOC.

Conclusion: Decreased expression and increased expression of miR-100 and miR-203 may be correlated with progression and poor prognosis of EOC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4891453PMC
June 2016

Investigation of serum levels and tissue expression of two genes IGFBP-2 and IGFBP-3 act as potential biomarker for predicting the progression and survival in patients with glioblastoma multiforme.

J Neurol Sci 2016 Jul 14;366:202-206. Epub 2016 May 14.

Department of Neurosurgery, AJA University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Identification of genetic copy number changes in glial tumors is of importance in the context of improved/refined diagnostic, prognostic procedures and therapeutic decision-making. Blood-derived biomarkers, therefore, would be useful as minimally invasive markers that could support diagnosis and enable monitoring of tumour growth and response to treatment.

Objective: The aim of this study was to evaluate the clinical significance of IGFBP-2/3 in glioblastoma multiforme (GBM) and their value as predictors of survival.

Methods: We examined the plasma levels of IGFBP-2 and IGFBP-3 using ELISA in patient suffering from GBM and controls groups. Furthermore, immunohistochemistry method was used to evaluate the expression levels of these markers.

Results: Preoperative plasma levels of IGFBP-2 and IGFBP-3 were markedly higher in glioblastoma patients (mean±SD: 521.5±164.2ng/ml; 402.4±126ng/ml) when compared with healthy controls (301.28±73.12; 244±89.5ng/ml; p<0.001). Immunohistochemical results indicated that the median H score for glioblastoma tissues was higher when compared with normal tissues. The mean scores for IGFBP-2 expression in glioblastoma was higher than normal tissues (p<0.001). Our result showed that the median H score for glioblastoma tissues was higher when compared with normal tissue for IGFBP-3 expression. The mean scores for glioblastoma tissues was higher than normal tissues (p<0.001). We also evaluated whether plasma IGFBP-2 and IGFBP-3 levels were related to clinical features. The plasma IGFBP-2 level was strongly linked to the patient's age (R=0.769, P=0.001) that were strongly increased in patients with older age (>65), (mean±SD: 594.36±33.3ng/ml). On the other hand, plasma IGFBP-3 level was not correlated with age (P=0.462), sex (P=0.532), and tumor size (P=0.245). Our findings indicated that the tissue IGFBP-2 level was also markedly correlated with the patient's age (R=0.612, P=0.015). On the other hand, tissue IGFBP-3 expression level was not correlated with age (P=0.472), sex (P=0.512), and tumor size (P=0.241). Kaplan-Meier survival and log-rank analysis suggested that patients with high plasma level of IGFBP-2 and tissue expression of IGFBP-2 had shorter overall survival than those with low levels (log-rank test P=0.027; P<0.001). Kaplan-Meier survival and log-rank analysis suggested that patients with high plasma level of IGFBP-3 and tissue expression of IGFBP-3 had shorter overall survival than those with low levels groups (log-rank test P=0.018; P<0.001).

Conclusion: These data suggest that plasma levels and tissue levels of IGFBP-2 and IGFBP-3 may be as potential biomarkers for predicting the progression and survival in patients with GBM.
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http://dx.doi.org/10.1016/j.jns.2016.05.018DOI Listing
July 2016

Immunohistochemical distinction of metastases of renal cell carcinoma with molecular analysis of overexpression of the chemokines CXCR2 and CXCR3 as independent positive prognostic factors for the tumorigenesis.

IUBMB Life 2016 08 6;68(8):629-33. Epub 2016 Jun 6.

Department of Pathology, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.

Renal cell carcinoma (RCC) represents, on average, over 90% of all malignancies of the kidney that occur in adults in both sexes. Chemokine receptors expression has been found in many kinds of cancer and at tumor metastasis site. We determined CXCR2 and CXCR3 expression in RCC by immunohistochemistry method and analyzed the prognostic value of these markers. Our finding demonstrated that CXCR3 were highly overexpressed in renal cancer tissues compared with those adjacent normal kidney tissues (P < 0.001). The results showed that high expression of CXCR3 was markedly correlated with metastasis (P = 0.021) and tumor stage (P = 0.031). CXCR2 were overexpressed in renal cancer tissues compared with those adjacent normal kidney tissues (P < 0.001). Our result showed that CXCR2 expression was correlated with high grade (P = 0.024), advanced stage (P = 0.029) and metastasis (P = 0.018). The log-rank test revealed that high CXCR2 and CXCR3 expressions are related to poorer overall survival (P < 0.001; P < 0.001). In conclusion, this study indicates the correlation of CXCR3 and CXCR3 with progression of RCC. In addition, high CXCR3 andCXCR2 expressions were correlated with shorter overall survival. © 2016 IUBMB Life, 68(8):629-633, 2016.
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http://dx.doi.org/10.1002/iub.1520DOI Listing
August 2016

Diagnostic investigations of DKK-1 and PDCD5 expression levels as independent prognostic markers of human chondrosarcoma.

IUBMB Life 2016 07 3;68(7):597-601. Epub 2016 Jun 3.

Department of Orthopedic and Trauma Surgery, Shahroud University of Medical Sciences, Shahroud, Iran.

In this study, we investigated the expression levels of Dickkopf-1 (DKK-1) and programmed cell death 5 (PDCD5) by using quantitative real-time PCR and immunohistochemistry in patients with chondrosarcoma. The DKK-1 mRNA levels were significantly higher in chondrosarcoma when compared with the corresponding nontumor tissues (mean ± SD: 4.23 ± 1.54; 1.54 ± 0.87; P = 0.001). PDCD5 mRNA levels were remarkably deceased in tumor tissues when compared with corresponding nontumor tissues (mean ± SD: 1.94 ± 0.73; 5.42 ± 1.73; P = 0.001). The high and moderate DKK-1 expressions were observed for 60% of chondrosarcoma samples in comparison with 27.5% of corresponding nontumor tissues (P  =  0.001). Moreover, low expression of PDCD5 was found in 67.5% of the tumor tissues when compared with the nontumor tissues (32.5%; P = 0.002). The results of this study showed that high DKK-1 expression levels were strongly related to MSTS stage (P = 0.011) and the advancement of histological grade (P < 0.001). Furthermore, the PDCD5 expression levels were correlated with histological grade (P < 0.001), MSTS stage (P = 0.016), and distant metastasis (P = 0.001). Kaplan-Meier survival and log-rank survival showed that patients with high DKK-1 levels and low PDCD5 levels were correlated with shorter overall survival (log-rank test P < 0.001). PDCD5 levels, histological grade, and tumor stage were independent predictors of overall survival. In conclusion, DKK-1 and PDCD5 can be independent predictors of overall survival in patients suffering from chondrosarcoma. © 2016 IUBMB Life, 68(7):597-601, 2016.
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http://dx.doi.org/10.1002/iub.1519DOI Listing
July 2016

Overexpression of ubiquitin-specific protease 2a (USP2a) and nuclear factor erythroid 2-related factor 2 (Nrf2) in human gliomas.

J Neurol Sci 2016 Apr 2;363:249-52. Epub 2016 Mar 2.

Department of Neurosurgery, AJA University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Gliomas are among the most frequent adult primary brain tumors. Recent studies have shown that there are novel opportunities for developing therapeutics by targeting the differentiation and self-renewal features of glioma.

Objective: The aim of this study was to evaluate the expression levels of USP2a an Nrf2 in patients with glioma and their association with prognosis of gliomas that was detected with immunohistochemical staining.

Methods: In this study, 40 patient's tissue samples with primary gliomas were collected between January 2009 and December 2013. MRI of patients was done before and within 24 h after surgery. USP2a and Nrf2 expression levels were examined by immunohistochemistry. Data were analyzed using the SPSS 16.0, X(2) test, log-rank test and Kaplan-Meier method.

Results: Immunohistochemistry indicated that USP2a expression was increased in glioma cells than normal brain tissues. The increased USP2a staining was markedly correlated with advanced tumor grade (P=0.02) and age (P=0.016). Our result showed that Nrf2 expression was significantly higher in glioma cells as compared to normal brain tissues. The high expression level of Nrf2 was markedly linked to age (P=0.007), and tumor grade (P=0.03). Kaplan-Meier survival and log-rank analysis indicated that patients with low expression of USP2a had longer overall survival than those with high levels (log-rank test P<0.001). Moreover, patients with high Nrf2 expression had shorter overall survival than those with low levels (log-rank test P<0.001). In the univariate analysis, the high expression of Nrf2 and USP2a (P=0.004; P=0.006), age (P=0.025), and tumor grade (P=0.001) were correlated with poor survival. Multivariate Cox proportional hazards model indicated that, high Nrf2 and USP2a staining (P=0.001; P=0.003), advanced tumor grade (P=0.01) and age (P=0.033) were independent predictor of overall survival.

Conclusion: In summary, the result of this study showed USP2a and Nrf2 may be as prognostic marker in patients with gliomas.
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http://dx.doi.org/10.1016/j.jns.2016.03.003DOI Listing
April 2016

Somatic CPEB4 and CPEB1 genes mutations spectrum on the prognostic predictive accuracy in patients with high-grade glioma and their clinical significance.

J Neurol Sci 2016 Apr 16;363:80-3. Epub 2016 Feb 16.

Department of Nuerosurgery, AJA University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Glioblastoma (grade IV glioma/GBM) is characterized by extremely aggressive invasion and proliferative nature.

Objective: The main goal of this study was to evaluate the expression patterns of CPEB1 and CPEB4 in glioma patients.

Methods: 41 paraffin-embedded tissue samples with glioma (WHO I-IV) were collected between January 2008 and December 2012 in Tehran, Iran. MRI of patients was done before and within 24 h after surgery and gliomas investigated using quantitative real-time PCR and immunohistochemistry. Kaplan-Meier survival and Cox regression were applied to assess the prognosis of patients.

Results: The mRNA level of CPEB4 was strongly increased in tumor tissues (0.67±3.154 vs. 1.671±0.51; P=0.001). Furthermore, CPEB1 mRNA was significantly decreased in tumor tissues compared to normal tissues (2.852±0.587 vs. 1.471±0.862; P=0.025). Our findings showed that CPEB4 levels was markedly increased in patients with advanced grade gliomas (P=0.003). In addition, CPEB1 mRNA levels were not associated with clinicopathological features. Of the 41 cases, high CPEB4 expression was found in 29 patients (70.73%), while 12 cases (29.26%) showed weak expression levels, while the protein expression of CPEB4 were remarkably weak in normal tissues (P=0.001). However, no correlation was found between expression levels of CPEB1 and clinicopathological characteristics. Kaplan-Meier survival and log-rank test indicated that high expression of CPEB4 was correlated with shorter overall survival (log-rank test P<0.001). Furthermore, low expression of CPEB1 was linked to shorter overall survival (log-rank test P=0.021). Multivariate Cox proportional hazards model showed that high CPEB1 (P=0.027), low CPEB4 expressions (P=0.021), and advanced tumor grade (P=0.036) were independent predictor of overall survival.

Conclusion: Our data indicated expressions levels of CPEB4 and CPEB1 are correlated with overall survival in patients with glioma.
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http://dx.doi.org/10.1016/j.jns.2016.02.032DOI Listing
April 2016

Evaluation of mRNA expression levels of IL-17A and IL-10 cytokines in cervical cancer.

Tumour Biol 2016 Aug 29;37(8):10149-53. Epub 2016 Jan 29.

Department of Gynecology, Khanevadeh Hospital, AJA University of Medical Sciences, Tehran, Iran.

In this study, we evaluated mRNA expression levels of interleukin (IL)-10/IL-17A by quantitative real-time PCR and their clinical importance in cervical cancer. The IL-10 mRNA levels were higher in cervical cancer tissues as compared with corresponding normal tissues (p < 0.05). Moreover, IL-17A mRNA was significantly increased in cervical cancer tissues than in normal tissues (p < 0.05). Moreover, the high expression level of IL-10 mRNA was markedly related to International League of Gynecology and Obstetrics (FIGO) stage (p = 0.001), but no significant association was found with other clinical factors including age, tumor size, histological grades, and lymph node metastasis. Moreover, high expression levels of IL-17A were not associated with patients' age, tumor size, FIGO stage, and histological grades while IL-17A expression was strongly linked to lymphatic metastasis (p = 0.001). These findings showed that IL-17A might have a crucial role in cervical cancer metastasis. Taken together, IL-17A expression was strongly linked to lymphatic metastasis, indicating that IL-17A might have a crucial role in cervical cancer metastasis. Moreover, our study suggested the association of IL-10 mRNA expression with clinical stage.
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http://dx.doi.org/10.1007/s13277-016-4882-8DOI Listing
August 2016

Evaluation of gene expression level of CDC5L and MACC1 in poor prognosis and progression of osteosarcoma.

Tumour Biol 2016 Jun 29;37(6):8153-7. Epub 2015 Dec 29.

Department of Emergency Medicine, Alborz University of Medical Sciences, Karaj, Iran.

Current evidences have indicated that osteosarcoma is strongly associated with abnormal genetic and epigenetic changes that lead to the abnormal expression of oncogenes or methylation of tumor suppressor genes. In the present study, MACC1 and CDC5L mRNA levels in the patients with osteosarcoma were evaluated using quantitative real-time PCR. Our results demonstrated that CDC5L mRNA levels were higher in tumor tissues than in adjacent normal tissues (2.713 ± 0.738 vs. 1.071 ± 0.629; P < 0.05). Moreover, MACC1 was upregulated in tumor bone tissues than in adjacent normal tissues (3.221 ± 0. 624 vs. 1.427 ± 0.456; P < 0.05). Our result demonstrated that high expression of CDC5L was significantly related to advanced TNM stage (P = 0.032). No significant difference was determined between CDC5L mRNA expression and other clinicopathological parameters including age, gender, tumor diameter, location, tumor grade, and histological type. In addition, overexpression of MACC1 was strongly correlated with advanced TNM stage (P = 0.027) and high tumor grade (P = 0.035). Our findings indicated that mRNA level of CDC5L is correlated with advanced TNM stage, and MACC1 may be involved in progression of osteosarcoma.
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http://dx.doi.org/10.1007/s13277-015-4726-yDOI Listing
June 2016

Expression and prognostic value of the aldehyde dehydrogenase 1 (ALDH1) and N-myc downstream regulated gene 2 (NDRG2) as potential markers in human astrocytomas.

Tumour Biol 2016 May 30;37(5):6261-5. Epub 2015 Nov 30.

Department of Neurosurgery, Bou Ali Hospital, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

In this study, immunohistochemical analysis was used to evaluate the expression of ALDH1 and NDRG2 in astrocytoma tissue samples and normal brain tissues. ALDH1 protein staining displayed that AlDH1 expression was not detectable in eight astrocytoma tissues (8/36) and in all of normal brain tissues. There was a significant difference between ALDH1 expression and WHO grades (P = 0.03). Furthermore, no correlation was determined between expression levels of ALDH1 and other clinicopathological characteristics including age, sex, and tumor size. Immunohistochemistry showed that a high level of NDRG2 protein expression was markedly detected in normal brain tissues and expression of NDRG2 protein was significantly decreased in astrocytoma tissues. There was a significant association between pathological grading and NDRG2 expression level (P < 0.001, Table 1), but no correlation was determined between expression levels of NDRG2 and other clinicopathological characteristics including age, sex, and tumor size. We also obtained detailed follow-up data and evaluated the association of ALDH1/NDRG2 expressions with overall survival. Kaplan-Meier survival and log-rank analysis indicated that the patients with high proportion of ALDH1-positive cells and low proportion of NDRG2-positive had shorter overall survival (P < 0.001; P = 0.001). Univariate analysis indicated that the high proportion of ALDH1-positive cells (P < 0.001), the low proportion of NDRG2-positive cells (P = 0.009), and the advanced grade (P < 0.005) were markedly linked to the prognosis in patients. Furthermore, in the multivariate analysis, ALDH1 cells' expression (P = 0.012), low proportion of NDRG2-positive cells (P = 0.025), and advanced grade (P < 0.03) were linked to poor overall survival. Our results suggest that NDRG2 expression is related to decreased survival rates and NDRG2 may be a potential marker in the astrocytoma prognosis. NDRG2 may be a potential marker in the astrocytoma prognosis. ALDH1 expression was related to advanced pathological grade and survival rate in astrocytoma patients.
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http://dx.doi.org/10.1007/s13277-015-4491-yDOI Listing
May 2016