Elsayed Metwally - Suez Canal University Faculty of Veterinary Medicine - Lecturer

Elsayed Metwally

Suez Canal University Faculty of Veterinary Medicine

Lecturer

Ismailia | Egypt

ORCID logohttps://orcid.org/0000-0002-7471-3148

Elsayed Metwally - Suez Canal University Faculty of Veterinary Medicine - Lecturer

Elsayed Metwally

Introduction

Primary Affiliation: Suez Canal University Faculty of Veterinary Medicine - Ismailia , Egypt

Experience

Sep 2012 - Oct 2017
Suez Canal University Faculty of Veterinary Medicine
Assistant Lecturer
Cytology and Histology Department
Dec 2009 - Sep 2012
Suez Canal University Faculty of Veterinary Medicine
Demonstrator
Cytology and Histology Department
Oct 2017
Suez Canal University Faculty of Veterinary Medicine
Lecturer
Cytology and Histology Department
Jul 2017
Institute of Genetics and Developmental Biology Chinese Academy of Sciences
Postdoctor
Laboratory of Molecular Developmental Biology

Publications

2Publications

13Reads

26Profile Views

Calcium-Activated Calpain Specifically Cleaves Glutamate Receptor IIA But Not IIB at the Neuromuscular Junction.

J Neurosci 2019 Apr 31;39(15):2776-2791. Epub 2019 Jan 31.

State Key Laboratory for Molecular and Developmental Biology, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China, and

Calpains are calcium-dependent, cytosolic proteinases active at neutral pH. They do not degrade but cleave substrates at limited sites. Calpains are implicated in various pathologies, such as ischemia, injuries, muscular dystrophy, and neurodegeneration. Despite so, the physiological function of calpains remains to be clearly defined. Using the neuromuscular junction of of both sexes as a model, we performed RNAi screening and uncovered that calpains negatively regulated protein levels of the glutamate receptor GluRIIA but not GluRIIB. We then showed that calpains enrich at the postsynaptic area, and the calcium-dependent activation of calpains induced cleavage of GluRIIA at Q788 of its C terminus. Further genetic and biochemical experiments revealed that different calpains genetically and physically interact to form a protein complex. The protein complex was required for the proteinase activation to downregulate GluRIIA. Our data provide a novel insight into the mechanisms by which different calpains act together as a complex to specifically control GluRIIA levels and consequently synaptic function. Calpain has been implicated in neural insults and neurodegeneration. However, the physiological function of calpains in the nervous system remains to be defined. Here, we show that calpain enriches at the postsynaptic area and negatively and specifically regulates GluRIIA, but not IIB, level during development. Calcium-dependent activation of calpain cleaves GluRIIA at Q788 of its C terminus. Different calpains constitute an active protease complex to cleave its target. This study reveals a critical role of calpains during development to specifically cleave GluRIIA at synapses and consequently regulate synaptic function.

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Source
http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.2213-1
Publisher Site
http://dx.doi.org/10.1523/JNEUROSCI.2213-17.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462441PMC
April 2019
12 Reads
6.344 Impact Factor

Expression of glial cells molecules in the optic nerve of adult dromedary camel (Camelus dromedarius): A histological and immunohistochemical analysis.

Anat Histol Embryol 2019 Jan 18;48(1):74-86. Epub 2018 Nov 18.

Jessore University of Science and Technology, Jessore, Bangladesh.

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Source
http://doi.wiley.com/10.1111/ahe.12413
Publisher Site
http://dx.doi.org/10.1111/ahe.12413DOI Listing
January 2019
1 Read
0.672 Impact Factor

Top co-authors

Sameh M Farouk
Sameh M Farouk

Suez Canal University

1
Yong Q Zhang
Yong Q Zhang

Institute of Genetics and Developmental Biology

1
Wenhua Li
Wenhua Li

Wuhan University

1
Qifu Wang
Qifu Wang

Institute of Genetics and Developmental Biology

1
Guoli Zhao
Guoli Zhao

Institute of Life Sciences

1