Publications by authors named "Elizabeth M Prescott"

3 Publications

  • Page 1 of 1

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p.

Proc Natl Acad Sci U S A 2004 Apr 8;101(16):6068-73. Epub 2004 Apr 8.

Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, United Kingdom.

We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol I termination occurs close to the Reb1p-dependent terminator in wild-type strains. However, deletion mutants for the 3' end-processing enzyme Rnt1p or the Rpa12p subunit of Pol I both show Pol I transcription in the spacer. For Deltarpa12, these spacer polymerases are devoid of nascent transcripts, suggesting they are immediately degraded. The homology of Rpa12p to the small subunit Rpb9p of Pol II and Rpc11p of Pol III, both implicated in transcriptional termination, points to a common termination mechanism for all three classes of RNA polymerase.
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http://dx.doi.org/10.1073/pnas.0401393101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC395924PMC
April 2004

Transcriptional collision between convergent genes in budding yeast.

Proc Natl Acad Sci U S A 2002 Jun 19;99(13):8796-801. Epub 2002 Jun 19.

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.

Transcriptional interference between genes and the regulatory elements of simple eukaryotes such as Saccharomyces cerevisiae is an unavoidable consequence of their compressed genetic arrangement. We have shown previously that with the tandem arranged genes GAL10 and GAL7, inefficient transcriptional termination of the upstream gene inhibits initiation of transcription on the downstream gene. We now show that transcriptional interference can occur also with S. cerevisiae RNA polymerase II genes arranged convergently. We demonstrate that when the GAL10 and GAL7 genes are rearranged in a convergent orientation, transcriptional initiation occurs at full levels. However, as soon as the two transcripts begin to overlap, elongation is restricted, resulting in a severe reduction in steady-state mRNA accumulation. This effect is observed only in cis arrangement, arguing against RNA-interference effects acting on the potential generation of antisense transcripts. These data reinforce the necessity of separating adjacent RNA polymerase II transcription units by efficient termination signals.
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http://dx.doi.org/10.1073/pnas.132270899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124378PMC
June 2002