Publications by authors named "Elif Yilmaz"

44 Publications

Survival after autologous versus allogeneic transplantation in patients with relapsed and refractory Hodgkin lymphoma.

Leuk Lymphoma 2021 May 14:1-8. Epub 2021 May 14.

Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

For relapsed Hodgkin lymphoma, salvage chemotherapy followed by auto-HCT is the standard of care. It is important to identify subpopulations who could benefit from allo-HCT. This retrospective analysis included 277 patients with rrHL who underwent first transplant with auto-HCT or allo-HCT between 2007-2017. Patients in the auto-HCT cohort ( = 218) were older, more likely to be in CR at the time of transplant and receive maintenance therapy post-transplant. Patients who underwent allo-HCT ( = 59) had a higher MSKCC relapse score. Factors associated with an inferior PFS and OS included early relapse, advanced stage, extranodal involvement and not achieving CR following salvage chemotherapy. After controlling for these 4 risk factors and MSKCC score, PFS ( = 0.112) or OS ( = 0.256) was not affected by the choice of transplant. In patients with ≥ 3 high risk features, the 4-year PFS was 51% in the allo-HCT vs. 39% ( = 0.107) in the auto-HCT cohort.
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http://dx.doi.org/10.1080/10428194.2021.1927016DOI Listing
May 2021

Testing the performance of European diatom indices for evaluating the ecological status in the Kızılırmak basin, Turkey: flowing waters.

Environ Sci Pollut Res Int 2021 Apr 9. Epub 2021 Apr 9.

Faculty of Arts and Sciences, Department of Biology, Dumlupınar University, Kütahya, Turkey.

The Kızılırmak River is the longest river (1.355 km) in Turkey and flows into the Black Sea. Main pressures in the basin are waste water discharges, widespread agriculture, livestock activities, and excessive salinity due to the natural formation. In this study, the performance of the most widely used European diatom indices (IPS, IDG, IBD, EPI-D, TDI, and TI) was tested statistically by comparing them with the indicators of organic pollution (biological oxygen demand and ammonium-nitrogen), eutrophication (orthophosphate and nitrate-nitrogen) and salinization (electrical conductivity) in the Kızılırmak basin, Turkey. For this purpose, a total of 97 diatom samples from 33 rivers were collected seasonally in 2014 and 215 species were identified. Principal component analysis was performed to evaluate the "general degradation" in the basin, and linear regression was used to calculate the correlations with diatom indices. As a result, EPI-D, IPS, IBD, and TI were strongly correlated indices, respectively, while IDG and TDI had the lowest correlations with general degradation. Only the EPI-D index was significantly correlated with all environmental variables. Our results showed that the EPI-D index can be used for the ecological status assessment in terms of phytobenthos in the Kızılırmak basin in line with the Water Framework Directive.
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http://dx.doi.org/10.1007/s11356-021-13282-1DOI Listing
April 2021

Comparative Assessment of Sera from Individuals after S-Gene RNA-Based SARS-CoV-2 Vaccination with Spike-Protein-Based and Nucleocapsid-Based Serological Assays.

Diagnostics (Basel) 2021 Mar 3;11(3). Epub 2021 Mar 3.

Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.

Due to the beginning of vaccination against COVID-19, serological discrimination between vaccine-associated humoral response and serology-based surveillance of natural SARS-CoV-2 infections as well as breakthrough infections becomes an issue of relevance. Here, we assessed the differentiated effects of the application of an RNA vaccine using SARS-CoV-2 spike protein epitopes on the results of both anti-spike protein-based serology (EUROIMMUN) and anti-nucleocapsid-based serology (VIROTECH). A total of 80 serum samples from vaccinees acquired at different time points after vaccination was assessed. While positive or borderline serological response in the anti-spike protein assay was observed for all samples (90% both IgG and IgA, 6.3% IgA only, 3.8% borderline IgG only), only a single case of a falsely positive IgM was observed for the anti-nucleocapsid assay as expected due to this assay's specificity. Positive anti-spike protein antibodies were already detectable in the second week after the first dose of vaccination, with higher titers after the second dose of the vaccine. In conclusion, the combined application of anti-spike protein-based serology and anti-nucleocapsid-based serology will provide a useful option for the discrimination of vaccination response and natural infection.
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http://dx.doi.org/10.3390/diagnostics11030426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998789PMC
March 2021

Nasopharyngeal carriage of Streptococcus pneumoniae in healthy children aged less than five years.

Vaccine 2021 04 17;39(15):2041-2047. Epub 2021 Mar 17.

Ege University School of Medicine, Department of Pediatric Infectious Diseases, İzmir, Turkey.

Purpose: In Turkey, pneumococcal conjugate vaccine (PCV) was introduced to the national immunization program as PCV7 in 2008, and was replaced with PCV13 in 2011. The aim of the study was to demonstrate the pneumococcal carriage rate and the serotype distribution in healthy children under 5 years in Turkey who were vaccinated with PCV13.

Methods: We conducted a cross-sectional study including the collection of questionnaire data and nasopharyngeal (NP) specimens among children aged <5 years from five centers from March 2019 to March 2020. Pneumococcal isolates were identified using optochin sensitivity and bile solubility. Serotyping was performed using a latex agglutination kit and Quellung reaction.

Results: NP swab samples were collected from 580 healthy children. The observed overall carriage rate was 17.8%. None of the hypothesised predictors of S. pneumoniae carriage, except maternal education level was statistically significant (p = 0.017). High maternal education level appeared to decrease the risk (lower vs. higher maternal education OR: 1.992 [95% CI; 1.089-3.643], p = 0.025). The overall NP S. pneumoniae carriage prevalence for the PCV13-vaccinated children was 17.8% (103/580). The most common serotypes detected were serotype 15B (n = 10, 9.7%), serotype 23F (n = 9, 8.7%), serotype 23A (n = 9, 8.7%), serotype 11A (n = 7, 6.7%), serotype 19F (n = 5, 4.8%) and serotype 15F (n = 5, 4.8%). Of the isolates, 28 (27.2%) were in PCV13 vaccine strains (VSs), and 75 (72.8%) strains were non-VS. The serotype coverage rate was 27.2% for PCV13.

Conclusion: The overall S. pneumoniae carriage rate was higher than in earlier studies from Turkey. Post-vaccine era studies from around the world have reported a decrease in VS serotypes and a 'serotype replacement' to non-VS serotypes, as we determined in our study.
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http://dx.doi.org/10.1016/j.vaccine.2021.03.028DOI Listing
April 2021

Seminal oxidation-reduction potential as a possible indicator of impaired sperm parameters in Turkish population.

Andrologia 2021 Mar 30;53(2):e13956. Epub 2020 Dec 30.

Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey.

Oxidative stress (OS) has been shown to have a key role in male infertility. Recently, a new measurement method has been developed to measure the overall oxidation-reduction potential (ORP) in a semen sample known as the MiOXSYS system. The aim of this study was to investigate the correlation of sperm parameters with oxidative stress levels determined by ORP and to evaluate whether the current limit is able to distinguish abnormal sperm parameters from normal ones in Turkish population. Semen samples of 121 patients who applied for infertility investigation were divided into two groups as (OS +; n:39) and (OS -; n:82). Semen parameters were compared between groups. Sperm concentration, total motility and progressive motility were found to be significantly lower in OS (+) patients compared to those OS (-), while immotile sperm count was significantly higher in OS (+) patients. Oxidative stress determined by MiOXSYS system was found to be related to reduced sperm parameters in Turkish population, which may be used as an indicator of poor sperm parameters and a support to routine semen analysis. In addition, recommended reference value was found to be reliable in distinguishing normal from impaired semen parameters.
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http://dx.doi.org/10.1111/and.13956DOI Listing
March 2021

Neuroprotective Effects of Curcumin-Loaded Emulsomes in a Laser Axotomy-Induced CNS Injury Model.

Int J Nanomedicine 2020 20;15:9211-9229. Epub 2020 Nov 20.

Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Beykoz, Istanbul, Turkey.

Purpose: Curcumin, a polyphenol isolated from the rhizomes of turmeric, holds great potential as a neuroprotective agent in addition to its anti-inflammatory and antioxidant characteristics. The poor bioavailability and low stability of curcumin are the greatest barriers to its clinical use. This study aims to investigate the neuroprotective effect of curcumin on axonal injury, by delivering the lipophilic polyphenol to a primary hippocampal neuron culture by means of a lipid-based drug delivery system, named emulsomes.

Methods: To study neuroregeneration ex vivo, an injury model was established through single-cell laser axotomy on hippocampal neurites. Upon treatment with curcumin-loaded emulsomes (CurcuEmulsomes), curcumin and CurcuEmulsome uptake into neurons was verified by three-dimensional Z-stack images acquired with confocal microscopy. Neuron survival after axonal injury was tracked by propidium iodide (PI) and Hoechst staining. Alterations in expression levels of physiological markers, such as anti-apoptotic marker Bcl2, apoptotic marker cleaved caspase 3, neuroprotective marker Wnt3a and the neuronal survival marker mTOR, were investigated by immunocytochemistry analyses.

Results: The results indicated significant improvement in the survival rate of injured neurons upon CurcuEmulsome treatment. Bcl2 expression was significantly higher for injured neurons treated with curcumin or CurcuEmulsome. Reduction in caspase 3 expression was seen in both curcumin and CurcuEmulsome treatment, whereas there were no significant changes in Wnt3a and mTOR expression.

Conclusion: The established laser-axotomy model was proven as a reliable methodology to study neurodegenerative models ex vivo. CurcuEmulsomes delivered curcumin to primary hippocampal neurons successfully. Treated with CurcuEmulsomes, injured hippocampal neurons benefit from the neuroprotective effects of curcumin, exhibiting a higher survival rate and increased anti-apoptotic marker levels.
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http://dx.doi.org/10.2147/IJN.S272931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685369PMC
January 2021

High levels of global genome methylation in patients with retinoblastoma.

Oncol Lett 2020 Jul 13;20(1):715-723. Epub 2020 May 13.

Department of Environmental Health Sciences, Mailman School of Public Health of Columbia University, New York, NY 10032, USA.

Retinoblastoma is a tumor of the embryonic neural retina in young children. The DNA methyltransferase 1 () gene has been demonstrated to be transcriptionally activated in cells lacking retinoblastoma 1 (). Thus, there is a direct interaction between and . The present study hypothesized that uncontrolled and expression may lead to a high level of global genome methylation causing a second hit or where both alleles are altered, in and/or inactivation of other genes in retinal cells. To test this, the global genome methylation levels were analyzed in 69 patients with retinoblastoma, as well as 26 healthy siblings and 18 healthy unrelated children as the control groups. Peripheral blood and tumor tissue samples were obtained from 32 patients. The expression levels of genes were also determined in cell lines. Based on the median levels of global genome methylation in patients, higher genome-wide methylation levels in peripheral blood were associated with a 3.33-fold increased risk for retinoblastoma in patients compared with all healthy controls (95% confidence interval, 0.98-11.35; P<0.0001). The level of global genome methylation and the expression of genes were increased in the WERI-RB-1 cell line, which has a mutated gene, compared with a wild-type -expressing cell line. These results supported the hypothesis that epigenetic alterations, as well as mutations in , may be associated with the oncogenesis and inheritance of retinoblastoma. The repression of genes that interact with , such as the gene family, may be important in patients with retinoblastoma with alterations in , and may serve a role in the treatment and regression of retinoblastoma.
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http://dx.doi.org/10.3892/ol.2020.11613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286142PMC
July 2020

Electrospun Polymers in Cartilage Engineering-State of Play.

Front Bioeng Biotechnol 2020 18;8:77. Epub 2020 Feb 18.

Regenerative, Modular & Developmental Engineering Laboratory, National University of Ireland Galway, Galway, Ireland.

Articular cartilage defects remain a clinical challenge. Articular cartilage defects progress to osteoarthritis, which negatively (e.g., remarkable pain, decreased mobility, distress) affects millions of people worldwide and is associated with excessive healthcare costs. Surgical procedures and cell-based therapies have failed to deliver a functional therapy. To this end, tissue engineering therapies provide a promise to deliver a functional cartilage substitute. Among the various scaffold fabrication technologies available, electrospinning is continuously gaining pace, as it can produce nano- to micro- fibrous scaffolds that imitate architectural features of native extracellular matrix supramolecular assemblies and can deliver variable cell populations and bioactive molecules. Herein, we comprehensively review advancements and shortfalls of various electrospun scaffolds in cartilage engineering.
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http://dx.doi.org/10.3389/fbioe.2020.00077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039817PMC
February 2020

Curcumin- and Piperine-Loaded Emulsomes as Combinational Treatment Approach Enhance the Anticancer Activity of Curcumin on HCT116 Colorectal Cancer Model.

Front Bioeng Biotechnol 2020 11;8:50. Epub 2020 Feb 11.

Regenerative and Restorative Medicine Research Center (REMER), Istanbul Medipol University, Istanbul, Turkey.

Combination chemotherapy, administrating two chemotherapeutic agents concurrently, comes into prominence, as the heterogeneity or the level of the disease necessitates a collaborative action. Curcumin, isolated from turmeric, and piperine, isolated from black long pepper, are two dietary polyphenols studied for their intrinsic anti-cancer properties against various cancer types including colorectal cancer (CRC). Furthermore, piperine improves the therapeutic effect of curcumin. Addressing this mutual behavior, this study combines curcumin and piperine within emulsome nanoformulations. Curcumin- (CurcuEmulsomes) and piperine-loaded emulsomes (PiperineEmulsomes) have established a uniform, stable, spherical dispersion with average diameters of 184.21 and 248.76 nm, respectively. The solid tripalmitin inner core achieved encapsulation capacities of up to 0.10 mg/ml curcumin and 0.09 mg/ml piperine content. While piperine treatment alone - in its both free and emulsome forms - showed no inhibition in the proliferation of HCT116 cells , its presence as the second drug agent enhanced curcumin's effect. Combination of 7 μM PiperineEmulsome and 25 μM CurcuEmulsome concentrations was found to be most effective with an inhibition of cell proliferation of about 50% viability. Cell cycle arrest at G2/M phase and induced apoptosis verified the improved anti-cancer characteristics of the therapy. While CurcuEmulsomes achieved a fourfold increase in Caspase 3 level, combination of treatment with PiperineEulsomes achieved a sixfold increase in the level of this apoptotic marker. Combinational treatment of HCT116 cells with CurcuEmulsomes and PiperineEmulsomes improved the anticancer activity of the compounds and highlighted the potential of the approach for further studies.
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http://dx.doi.org/10.3389/fbioe.2020.00050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026030PMC
February 2020

The quality of ECG data acquisition, and diagnostic performance of a novel adhesive patch for ambulatory cardiac rhythm monitoring in arrhythmia detection.

J Electrocardiol 2019 May - Jun;54:28-35. Epub 2019 Feb 28.

Innovation Department, Arçelik A.Ş., Istanbul, Turkey.

Background: Short and long ambulatory electrocardiographic monitoring with different systems is a widely used method to detect cardiac arrhythmias. In this study, we aimed to evaluate the effectiveness of a novel monitoring device on cardiac arrhythmia detection.

Methods: We used two different protocols to evaluate device performance. For the first one, 36 healthy subjects were enrolled. The standard 12‑lead, 24-h Holter monitoring and the novel single lead electrocardiogram (ECG) Patch Monitor (EPM) device (BeyondCare®, Rooti Labs Ltd., Taipei, Taiwan) were simultaneously applied to all subjects for 24 h. The quality of ECG data acquisition of novel system was compared to that of standard Holter. The second phase included 73 patients that were referred from our outpatient arrhythmia clinic for evaluation of their symptoms relevant to the cardiac arrhythmias. Advanced algorithms, statistical methods (cross-correlation method, Pearson's correlation coefficient, Bland-Altman plots) were used to process and verify the acquired data.

Results: The overall average beat per minute correlation between BeyondCare® and standard 12‑lead Holter was found 98% in 33 healthy subjects. The mean percentage of invalid measurements in BeyondCare® was 1.6% while the Holter's was 1.7%. In the second protocol of the study, prospective data from 67 patients who were referred for evaluation of their symptoms relevant to cardiac arrhythmias, showed that the mean BeyondCare® wear time was 4.7 ± 0.5 days out of five total days per protocol. The mean analyzable wear time was 93.6%. The water-resistant design enabled 73.5% of the participants to take a shower. 7.3% of participants had minor skin irritations related to the electrodes. Among the patients with detected arrhythmia (40.2% of all patients), 29.6% had their first arrhythmia after the initial two days period. A clinically significant pause was detected in one patient, ventricular tachycardia was detected in four patients, and supraventricular tachycardia was detected in 15 patients. Paroxysmal atrial fibrillation was identified in seven patients. Three of them had their first episodes after the second day of monitoring.

Conclusion: BeyondCare® Patch was well-tolerated and allowed prolonged time periods for continuous ECG monitoring, may result in an improvement in clinical accuracy and detection of arrhythmias by cloud-based artificial intelligence operating system.
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http://dx.doi.org/10.1016/j.jelectrocard.2019.02.012DOI Listing
September 2020

Molecular signatures of chronic periodontitis in gingiva: A genomic and proteomic analysis.

J Periodontol 2019 06 20;90(6):663-673. Epub 2019 Feb 20.

Department of Medical Genetics, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey.

Background: To elucidate molecular signatures of chronic periodontitis (CP) using gingival tissue samples through omics-based whole-genome transcriptomic and whole protein profiling.

Methods: Gingival tissues from 18 CP and 25 controls were analyzed using gene expression microarrays to identify gene expression patterns and the proteins isolated from these samples were subjected to comparative proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The data from transcriptomics and proteomics were integrated to reveal common shared genes and proteins.

Results: The most upregulated genes in CP compared with controls were found as MZB1, BMS1P20, IGLL1/IGLL5, TNFRSF17, ALDH1A1, KIAA0125, MMP7, PRL, MGC16025, ADAM11, and the most upregulated proteins in CP compared with controls were BPI, ITGAM, CAP37, PCM1, MMP-9, MZB1, UGTT1, PLG, RAB1B, HSP90B1. Functions of the identified genes were involved cell death/survival, DNA replication, recombination/repair, gene expression, organismal development, cell-to-cell signaling/interaction, cellular development, cellular growth/proliferation, cellular assembly/organization, cellular function/maintenance, cellular movement, B-cell development, and identified proteins were involved in protein folding, response to stress, single-organism catabolic process, regulation of peptidase activity, and negative regulation of cell death. The integration and validation analysis of the transcriptomics and proteomics data revealed two common shared genes and proteins, MZB1 and ECH1.

Conclusion: Integrative data from transcriptomics and proteomics revealed MZB1 as a potent candidate for chronic periodontitis.
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http://dx.doi.org/10.1002/JPER.18-0477DOI Listing
June 2019

Hepatoprotective Role of Thymol in Drug-Induced Gastric Ulcer Model.

Ann Hepatol 2018 Oct;17(6):980-991

Department of Biology, Faculty of Science, Ataturk University, Erzurum, TURKEY.

Introduction And Aim: Indo is widely one of the non-steroidal anti-inflammatory drugs and one of the common toxic effects of this drug is hepatic failure. Thymol is a monoterpene phenol with many different pharmacological activities. However, up to now its hepatoprotective effects on Indo-induced gastric ulcer model in rats have not been explored yet.

Material And Methods: Thirty five Sprague-Dawley rats were divided into seven groups: control, ulcer control (30 mg/kg Indo), Indo + reference standard (50 mg/kg Rantidine), Indo + Thymol (75, 100, 250 and 500 mg/kg) groups. 10 minutes after the induction of ulcer with Indo; Thymol was orally administered to the rats. Liver function enzymes (AST, ALT and LDH) were measured from serum samples. TOS/TAC, TNF-α and PGE2 levels, eNOS and Caspase-3 activity were assessed from tissue homogenate samples. In addition, histopathologic analysis on liver sections was performed.

Results: Indo significantly increased the levels of hepatic enzymes, TNF-α and eNOS, and caspase-3 activation, while decreased PGE2 levels. Furthermore, it induced oxidative stress as evidenced by elevated TOS and decreased TAC levels. However, Thymol treatment induced a significant improvement in these parameters, especially in 250 mg/kg dose. On the other hand, treatment with Thymol 500 mg/kg dramatically affected the parameters much worse than the Indo treated group.

Conclusion: The findings of the current study demonstrated that Thymol administration significantly ameliorated liver injury due to Indo toxicity. This effect of Thymol (250 mg/kg) may be mediated by its anti-oxidative or anti-inflammatory effect, and up-regulation the synthesis of PGE2.
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http://dx.doi.org/10.5604/01.3001.0012.7198DOI Listing
October 2018

Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients.

Zygote 2018 Aug 17;26(4):308-313. Epub 2018 Sep 17.

1Istanbul Medipol University,International School of Medicine,İstanbul,Turkey.

SummaryThe negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis of the process. The aim of the present study was to investigate the possible effects of sperm cryopreservation on main apoptotic signs including DNA fragmentation and caspase-3 activation and to determine if these effects vary according to sperm parameters. Sperm samples of 72 patients were cryopreserved. The patients were sub-grouped as normozoospermic or non-normozoospermic patients according to their semen parameters. DNA fragmentation rates and caspase-3 activation levels were analyzed before and after cryopreservation in both groups. Mean DNA fragmentation rate was increased significantly from 23.98% in neat semen samples to 27.34% after cryopreservation (P = 0.03). DNA fragmentation rates were slightly higher in non-normozoospermic patients compared with the normozoospermic patients in both the neat semen and after cryopreservation (23.25 and 24.71% vs. 26.32 and 28.36%, respectively) although the difference obtained were not statistically significant. An increasing trend for caspase-3 activations (0.093 vs. 0.116) was observed after cryopreservation but the differences were not statistically significant. Caspase-3 activation was found to be slightly higher in non-normozoospermic patients both in the neat semen and after cryopreservation compared with the normozoospermic patients but the differences were not statistically significant. Caspase-3 expression was also shown using immunocytochemistry in both fresh ejaculated sperm and thawed sperm after cryopreservation but at different localizations. The cryopreservation process had detrimental effects on sperm quality but the quality of the sperm samples was not adversely effective for the apoptotic markers including DNA fragmentation and caspase-3 activation patterns. In fact, it was the cryopreservation process itself that adversely effected the above apoptotic markers and apoptosis. It was concluded therefore that sperm cell cryopreservation triggers apoptosis after thawing and this process adversely affects semen parameters.
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http://dx.doi.org/10.1017/S0967199418000254DOI Listing
August 2018

Sugammadex-Induced Hypersensitivity Reaction in a Pediatric Patient.

Turk J Anaesthesiol Reanim 2018 Feb 1;46(1):66-68. Epub 2018 Feb 1.

Department of Anaesthesiology, Trakya University School of Medicine, Edirne, Turkey.

We report a case of a 3-year-old boy who administered sugammadex and developed an allergic reaction several minutes after the administration. He developed an increase in airway pressures and a decrease in peripheral oxygen saturation; auscultation revealed widespread wheezing in the lungs. He was successfully treated with immediate administration of methylprednisolone, pheniramine, and theophylline. We assumed an allergic reaction to sugammadex based on the clinical condition of the patient.
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http://dx.doi.org/10.5152/TJAR.2018.04696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858893PMC
February 2018

Early initiation and exclusive breastfeeding: Factors influencing the attitudes of mothers who gave birth in a baby-friendly hospital.

Turk J Obstet Gynecol 2017 Mar 15;14(1):1-9. Epub 2017 Mar 15.

Dr. Sami Ulus Maternity and Child Health and Diseases Training and Research Hospital, Clinic of Obstetrics and Gynecology, Ankara, Turkey.

Objective: To investigate the initation time of breastfeeding, exclusive breastfeeding rates, and complementary feeding practices during the first six months of life among mothers who gave birth in a baby-friendly hospital.

Materials And Methods: A cross-sectional study was conducted with 350 mothers. Demographic characteristics, obstetric history and information about breastfeeding initiation were collected at the hospital. Information about factors affecting breastfeeding duration and feeding practices of the infants were obtained at the end of six months.

Results: Some 97.4% of the mothers initiated breastfeeding, 60.1% within the first hour. Exclusive breastfeeding was maintained for six months in 38.9%. Low education levels of mother/father, random breastfeeding, rare breastfeeding at night, nipple problems, bottle/pacifier use, and lack of social support were found associated with early cessation. Planned pregnancy [odds ratio (OR=2.02)] and vaginal delivery (OR=0.3) were found as the most important factors in early initiation, whereas antepartum breastfeeding education (OR=7.17) was the most important factor for exclusive breastfeeding duration in the logistic analysis. More than half (61.1%) of the infants were partially/bottle fed for six months; the most common reason was the belief that breast milk was insufficient.

Conclusion: Efforts to encourage mothers and society to breastfeed exclusively should be made as part of a primary public health strategy to prevent early cessation of breastfeeding.
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http://dx.doi.org/10.4274/tjod.90018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558311PMC
March 2017

Elevated red blood cell distribution width is associated with intrahepatic cholestasis of pregnancy.

Ginekol Pol 2017 ;88(2):75-80

Zekai Tahir Burak Women's Health Care, Training and Research Hospital, Ankara, Turkey.

Objectives: Intrahepatic cholestasis of pregnancy is the most common pregnancy specific liver disease and related with adverse maternal and perinatal outcome. Red blood cell distribution width, an anisocytosis marker in a complete blood count, has been used as an inflammation marker in various diseases. However the association of red blood cell distribution width with intrahepatic cholestasis of pregnancy is unknown. We aimed to evaluate the relationship between red blood cell distribution width and intrahepatic cholestasis of pregnancy.

Material And Methods: Ninety pregnant women with intrahepatic cholestasis of pregnancy and ninety healthy pregnant women were included in the study. Their clinical and laboratory characteristics including red blood cell distribution width, liver function tests, fasting and postprandial bile acid concentrations were analyzed.

Results: Serum red blood cell distribution width cell levels were significantly higher in pregnants with intrahepatic cholestasis of pregnancy than healthy pregnants. We also demonstrated that red blood cell distribution Width levels were higher in severe disease than mild disease and was significantly correlated with fasting and postprandial bile acid concentration in intrahepatic cholestasis of pregnancy group.

Conclusions: Our study showed that red blood cell distribution width, an easy and inexpensive marker; were associated with intrahepatic cholestasis of pregnancy and can be used as a diagnostic and prognostic marker in intrahepatic cholestasis of pregnancy.
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http://dx.doi.org/10.5603/GP.a2017.0015DOI Listing
July 2018

Influence of Behçet's disease on first and second trimester serum screening markers.

J Obstet Gynaecol Res 2017 Mar 17;43(3):511-515. Epub 2016 Dec 17.

Department of Perinatology, Zekai Tahir Burak Women's Health Education and Training Hospital, Ankara, Turkey.

Aim: Behçet's disease (BD) is a rare and multisystemic vasculitis disease. In this study, we investigated whether BD had any effect on the biochemical components of first and second trimester aneuploidy screening tests.

Methods: A case-control retrospective study was conducted with 32 pregnant women with BD and 60 healthy pregnant women as controls. All pregnant womens' first trimester maternal serum pregnancy-associated plasma protein-A, free β-human chorionic gonadotropin and second trimester serum alpha-fetoprotein, unconjugated estriol and total human chorionic gonadotropin levels were examined from medical records. First and second serum screening markers were compared between pregnancies with BD and without.

Results: There was no difference in age, body mass index and obstetric history between the groups. No significant difference was observed between the groups in terms of first and second trimester serum screening test results in the absence of aneuploidy or neural tube defect. Gestational age at birth, birth weight and neonatal intensive care admission rate were also similar between the groups.

Conclusion: Both first and second serum screening tests for Down syndrome may be recommended to pregnant women with BD without the need to readjust these markers. Pregnancy with BD was not associated with adverse perinatal outcome with respect to gestational age at birth or birth weight.
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http://dx.doi.org/10.1111/jog.13237DOI Listing
March 2017

Red blood cell distribution width: A simple parameter in preeclampsia.

Pregnancy Hypertens 2016 Oct 6;6(4):285-287. Epub 2016 May 6.

Dr. Sami Ulus Women's and Children's Health Training and Research Hospital, Department of Obstetrics and Gynaecology Ankara, Turkey.

Objective: Preeclampsia is a serious disease and a leading cause of maternal and perinatal mortality and morbidity. Red blood cell distribution width (RDW), a measure of anisocytosis, is used as an inflammation marker in hypertension and cardiovascular diseases. Although the relationship between RDW and hypertension has been well documented, the association between preeclampsia and RDW is not clear. We aimed to investigate the relationship between RDW and preeclampsia and its severity.

Materials And Methods: One hundred eighteen pregnant women with preeclampsia and one hundred twenty uncomplicated pregnant women were included in the study. Blood samples for routine CBC and RDW levels were analyzed.

Results: The RDW values were significantly higher in preeclampsia group compared with the control group (15.23±1.96 vs 14.48±1.70, p<0.05). We also confirmed that RDW levels were significantly higher in severe preeclampsia group than mild preeclampsia group in subgroup analyses (15.08±2.07, 15.92±1.99, p<0.05).

Conclusion: Our study showed that there is an association between RDW and preeclampsia and also its severity. RDW, a marker which is easy, inexpensive and calculated as a part of blood cell count, can be used as a significant diagnostic and prognostic marker in patient with preeclampsia like the other cardiovascular diseases.
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http://dx.doi.org/10.1016/j.preghy.2016.05.001DOI Listing
October 2016

Comparison of elastosonography and digital examination of cervix for consistency to predict successful vaginal delivery after induction of labor with oxytocin.

J Matern Fetal Neonatal Med 2017 Dec 1;30(23):2795-2799. Epub 2016 Dec 1.

b Department of Obstetrics and Gynecology , Dr. Sami Ulus Women's Health Training and Research Hospital , Ankara , Turkey.

Objective: To compare elastosonography and digital examination of cervix for consistency in the prediction of successful vaginal delivery.

Methods: A total of 64 pregnant women with the indication of induction of labor (IOL) were enrolled to the study. The uterine cervix is evaluated before and after the IOL with elastosonography and digital examination for consistency and sonography for length. Methods were compared in regard to the prediction of successful vaginal delivery.

Results: The median of gestational age was 41.00 (IQR = 2.32). Out of 64 participants, 40 (62.5%) had vaginal delivery and 24 (37.5%) had cesarean delivery. The preinduction and postinduction elastosonographic indices were insignificant in delivery groups. The preinduction and postinduction evaluations of cervical consistency with digital examination were significant within vaginal delivery group (p = 0.046), whereas it was insignificant within cesarean delivery group and between the delivery groups. The preinduction and postinduction Bishop scores were significant within vaginal delivery group (p = 0.005), whereas it was insignificant within cesarean delivery group and between the delivery groups. Postinduction Bishop score was significant between the delivery groups.

Conclusion: Evaluation of cervix for consistency with either elastosonography or digital examination was found to be insignificant in prediction of successful vaginal delivery after IOL with oxytocin.
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http://dx.doi.org/10.1080/14767058.2016.1263296DOI Listing
December 2017

Relationship between anemia and depressive mood in the last trimester of pregnancy.

J Matern Fetal Neonatal Med 2017 Apr 17;30(8):977-982. Epub 2016 Jun 17.

a Department of Obstetrics and Gynaecology , Dr. Sami Ulus Maternity and Womens' Health Training and Research Hospital , Ankara , Turkey.

Objective: To compare the relationship between the severity of anemia and depressive mood in the last trimester of pregnancy.

Methods: A cross-sectional study, enrolled a total of 450 pregnant women who attended the antenatal clinics in their third trimester for their routine antenatal follow-up. Depressive symptoms were assessed by the Edinburgh Postnatal Depression Scale. The study group was divided into two groups according to presence of anemia; anemic group (Hb < 11 gr/L; n = 150) and non-anemic group (Hb ≥ 11 gr/L; n = 300) and depression scores were compared.

Results: One hundred and fourteen (25.3%) women scored ≥13 points which were considered the cutoff value for depression on the EPDS. Anemia frequency was found as 33.3%. The total EPDS score was significantly higher in the anemic group (EPDS score 11 [min-max 0-29]) compared with the non-anemic group (EPDS score 7 [min-max 0-21]) (p = 0.000). Multiple regression analysis also revealed that serum Hb level was an independent factor for antenatal depressive mood.

Conclusions: As anemia is associated with higher depressive symptom levels, it should be carefully considered during pregnancy. Prospective studies are needed to confirm our results.
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http://dx.doi.org/10.1080/14767058.2016.1194389DOI Listing
April 2017

Factors Associated with Breastfeeding Initiation and Exclusive Breastfeeding Rates in Turkish Adolescent Mothers.

Breastfeed Med 2016 08 16;11(6):315-320. Epub 2016 May 16.

1 Department of Obstetrics and Gynecology, Sami Ulus Women's Health/Children Education and Research Hospital , Ankara, Turkey .

Objective: This study aimed to determine the early initiation time of breastfeeding and exclusive breastfeeding (EBF) rates during the first 6 months and the main factors affecting EBF practice in Turkish adolescent mothers.

Study Design And Methods: A cross-sectional study was conducted with 200 adolescent mothers who were raising 6 to 24 month-old children. A face-to-face interview was conducted for sociodemographic characteristics, obstetric history, information about the baby, information about breastfeeding, and the factors affecting breastfeeding. We detected breastfeeding prevalence as well as its initiation time and duration.

Results: The mean age of the patients was 17.9 ± 1.15 years. The percentage of patients who started breastfeeding within 1 hour was 45.5%. Approximately, 74% of the adolescent mothers provided breastfeeding before any type of formula as the baby's first food. Women who initiated breastfeeding earlier had planned pregnancies, educated for breastfeeding, delivered vaginally, and had boy infants. The mothers who started breastfeeding earlier fed their babies significantly more frequently at night, had longer EBF times, and had longer total breastfeeding times than the mothers who started breastfeeding later than 2 hours. Planned pregnancy, vaginal delivery, and having a boy infant were significantly associated with early breastfeeding. Age, planned pregnancy, postpartum education, frequent breastfeeding at night, and formula initiation time were predictors of EBF.

Conclusions: Adolescent pregnants may start breastfeeding earlier when had planned pregnancy, educated and encouraged for breastfeeding from the family and medical staff. Therefore, strategies should be formed to improve breastfeeding programs.
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http://dx.doi.org/10.1089/bfm.2016.0012DOI Listing
August 2016

Molecular etiology of arthrogryposis in multiple families of mostly Turkish origin.

J Clin Invest 2016 Feb 11;126(2):762-78. Epub 2016 Jan 11.

Background: Arthrogryposis, defined as congenital joint contractures in 2 or more body areas, is a clinical sign rather than a specific disease diagnosis. To date, more than 400 different disorders have been described that present with arthrogryposis, and variants of more than 220 genes have been associated with these disorders; however, the underlying molecular etiology remains unknown in the considerable majority of these cases.

Methods: We performed whole exome sequencing (WES) of 52 patients with clinical presentation of arthrogryposis from 48 different families.

Results: Affected individuals from 17 families (35.4%) had variants in known arthrogryposis-associated genes, including homozygous variants of cholinergic γ nicotinic receptor (CHRNG, 6 subjects) and endothelin converting enzyme-like 1 (ECEL1, 4 subjects). Deleterious variants in candidate arthrogryposis-causing genes (fibrillin 3 [FBN3], myosin IXA [MYO9A], and pleckstrin and Sec7 domain containing 3 [PSD3]) were identified in 3 families (6.2%). Moreover, in 8 families with a homozygous mutation in an arthrogryposis-associated gene, we identified a second locus with either a homozygous or compound heterozygous variant in a candidate gene (myosin binding protein C, fast type [MYBPC2] and vacuolar protein sorting 8 [VPS8], 2 families, 4.2%) or in another disease-associated genes (6 families, 12.5%), indicating a potential mutational burden contributing to disease expression.

Conclusion: In 58.3% of families, the arthrogryposis manifestation could be explained by a molecular diagnosis; however, the molecular etiology in subjects from 20 families remained unsolved by WES. Only 5 of these 20 unrelated subjects had a clinical presentation consistent with amyoplasia; a phenotype not thought to be of genetic origin. Our results indicate that increased use of genome-wide technologies will provide opportunities to better understand genetic models for diseases and molecular mechanisms of genetically heterogeneous disorders, such as arthrogryposis.

Funding: This work was supported in part by US National Human Genome Research Institute (NHGRI)/National Heart, Lung, and Blood Institute (NHLBI) grant U54HG006542 to the Baylor-Hopkins Center for Mendelian Genomics, and US National Institute of Neurological Disorders and Stroke (NINDS) grant R01NS058529 to J.R. Lupski.
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http://dx.doi.org/10.1172/JCI84457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731160PMC
February 2016

Nausea and Vomiting in Early Pregnancy of Adolescents: Relationship with Depressive Symptoms.

J Pediatr Adolesc Gynecol 2016 Feb 2;29(1):65-8. Epub 2015 Jul 2.

Dr Sami Ulus Women's and Children's Health Teaching and Research Hospital, Department of Obstetrics and Gynecology, Ankara, Turkey.

Study Objective: To determine the relationship between severity of nausea and vomiting during pregnancy (NVP) and depressive symptoms in pregnant adolescents.

Design: Prospective cross-sectional study.

Setting: A maternity research hospital outpatient clinic, Ankara, Turkey.

Participants: A total of 200 pregnant adolescents.

Interventions And Main Outcome Measures: Demographic features and obstetric histories of the participants were assessed. The Rhodes test was performed to determine nausea and vomiting severity in a face-to-face interview, and the self-reported Edinburgh Postnatal Depression Scale was administered with supervision.

Results: The Rhodes test results showed that 52/200 patients (26%) were classified with none, 83/200 patients (41.5%) with mild, 48/200 patients (24.0%) with moderate, and 17/200 patients (8.5%) with severe symptoms. The mean depression score in the severe vomiting group was significantly higher than that in the no NVP and mild NVP groups (P = .028 and .041, respectively). No differences were found between the other groups.

Conclusion: Severe nausea and vomiting was associated with greater depressive symptom severity in pregnant adolescents.
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http://dx.doi.org/10.1016/j.jpag.2015.06.010DOI Listing
February 2016

Gene-Expression Profiles in Generalized Aggressive Periodontitis: A Gene Network-Based Microarray Analysis.

J Periodontol 2016 Jan 2;87(1):58-65. Epub 2015 Jul 2.

Department of Medical Genetics, Faculty of Medicine, Kocaeli University.

Background: In this study, molecular biomarkers that play a role in the development of generalized aggressive periodontitis (GAgP) are investigated using gingival tissue samples through omics-based whole-genome transcriptomics while using healthy individuals as background controls.

Methods: Gingival tissue biopsies from 23 patients with GAgP and 25 healthy individuals were analyzed using gene-expression microarrays with network and pathway analyses to identify gene-expression patterns. To substantiate the results of the microarray studies, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to assess the messenger RNA (mRNA) expression of MZB1 and DSC1. The microarrays and qRT-PCR resulted in similar gene-expression changes, confirming the reliability of the microarray results at the mRNA level.

Results: As a result of the gene-expression microarray studies, four significant gene networks were identified. The most upregulated genes were found as MZB1, TNFRSF17, PNOC, FCRL5, LAX1, BMS1P20, IGLL5, MMP7, SPAG4, and MEI1; the most downregulated genes were found as LOR, LAMB4, AADACL2, MAPT, ARG1, NPR3, AADAC, DSC1, LRRC4, and CHP2.

Conclusions: Functions of the identified genes that were involved in gene networks were cellular development, cell growth and proliferation, cellular movement, cell-cell signaling and interaction, humoral immune response, protein synthesis, cell death and survival, cell population and organization, organismal injury and abnormalities, molecular transport, and small-molecule biochemistry. The data suggest new networks that have important functions as humoral immune response and organismal injury/abnormalities. Future analyses may facilitate proteomic profiling analyses to identify gene-expression patterns related to clinical outcome.
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http://dx.doi.org/10.1902/jop.2015.150175DOI Listing
January 2016

uPAR, IL-33, and ST2 values as a predictor of subclinical chorioamnionitis in preterm premature rupture of membranes.

J Interferon Cytokine Res 2013 Dec;33(12):778-82

1 Department of Obstetrics and Gynecology, Sami Ulus Medical and Research Hospital , Ankara, Turkey .

Preterm premature rupture of membranes (PPROM) is defined as rupture of membrane that happens before the onset of labor and 37 weeks. Subclinical intrauterine infection is major etiological factor in the pathogenesis that increases mortality and morbidity associated with PPROM. This study was performed to evaluate the levels of maternal serum urokinase plasminogen activator receptor (uPAR), ST2 and interleukin (IL)-33 in PPROM and its relation with maternofetal infectious and morbidity. A total of 74 pregnant women, of which 49 with PPROM between 24 and 34 weeks gestation, and 25 normal pregnant women without PPROM were included in the study. Study group was seperated into 2 subgroups as PPROM and PPROM-histological chorioamnionitis (PPROM-HC). The blood samples were taken before the any medication. The mean serum IL-33, ST2, and uPAR values in the PPROM-HC group were significantly higher than PPROM and control group. The cut-off values of IL-33, ST2, and uPAR were determined as 5.2, 2 and 6.4 ng/mL, respectively. A cut-off value of IL-33 of 5.2 ng/mL, the cut-off of ST2 of 2 ng/mL and the cut-off of uPAR of 6.4 ng/mL showed similar sensitivity, specificity to IL-6 and the better sensitivity and specificity as compared to C-reactive protein and total leucocyte count in predicting infection in PPROM. We evaluated the predictive value of uPAR, ST2 and IL-33 in PPROM and we concluded that all of them can be used as reliable biomarkers to determine infection without any clinical signs but it is necessary to be studied in different cohort groups and infectious diseases.
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http://dx.doi.org/10.1089/jir.2012.0151DOI Listing
December 2013

The relationship between depressive symptoms and obstructive sleep apnea in pediatric populations: a meta-analysis.

J Clin Sleep Med 2013 Nov 15;9(11):1213-20. Epub 2013 Nov 15.

Department of Child and Adolescent Psychiatry, Drexel University College of Medicine, Philadelphia, PA.

Background: A higher incidence of depressive disorders and symptoms has been suggested among children suffering from obstructive sleep apnea (OSA). Yet, the extent to which OSA is related to increased depression is unclear.

Objectives: To evaluate (a) the relationship between depressive symptoms and OSA in pediatric populations, and (b) the efficacy of adenotonsillectomy (AT) for decreasing depressive symptoms among children with OSA.

Methods: A meta-analysis was conducted to assess the relationship between depressive symptoms and OSA, and the efficacy of AT for decreasing depressive symptoms. Studies reporting depressive symptoms of children with OSA through January 2013 were included.

Results: Eleven studies assessed depressive symptoms in both children diagnosed with OSA (n = 894) and a comparison group (n = 1,096). A medium relationship was found between depressive symptoms and OSA (Hedges' g = 0.43, 95% CI: 0.22-0.64; p = 0.0005). Addressing the second question, 9 studies (n = 379 children) examined depressive symptoms pre- and post-AT. A medium improvement in depressive symptoms was found at follow-up (Hedge's g = 0.41, 95% CI: 0.20-0.62; p ≤ 0.001).

Conclusion: Our findings suggest that depressive symptoms are higher among children with OSA. Therefore, patients with depressive symptomatology should receive screening for sleep disordered breathing. Treatment of OSA with AT might decrease clinical symptoms of depression, reduce pharmacotherapy, improve sleep patterns, and promote better health.
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http://dx.doi.org/10.5664/jcsm.3178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805811PMC
November 2013

Proadrenomedullin and serum amyloid A as a predictor of subclinical chorioamnionitis in preterm premature rupture of membranes.

J Interferon Cytokine Res 2013 Nov 6;33(11):694-9. Epub 2013 Sep 6.

1 Department of Obstetrics and Gynecology, Sami Ulus Medical and Research Hospital , Ankara, Turkey .

Preterm premature rupture of membranes (PPROM) is defined as a spontaneous membrane rupture that occurs before the onset of labor and 37 weeks gestation. Subclinical intrauterine infection has been suggested as a very important etiological factor in the pathogenesis and subsequent morbidity related with PPROM. This study was performed to assess the levels of maternal proadrenomedullin (pro-ADM) and serum amyloid A (AA) in PPROM and its association with fetomaternal infectious morbidity. A total of 63 pregnant women, of which 43 with PPROM between 24 and 34 weeks gestation and 20 normal pregnant women without PPROM were included in the study. The study group was separated into 2 subgroups as PPROM and PPROM-histological chorioamnionitis (PPROM-HC). The blood samples were taken before the administration of any medication. The mean serum interleukin-6 (IL-6), AA, and pro-ADM values in the PPROM-HC group were significantly higher than the PPROM and control group. The cutoff values of pro-ADM and AA were determined as 4.2 nM and 69 μg/mL, respectively. Both of them showed similar sensitivity, specificity to IL-6 and a better sensitivity and specificity as compared to C-reactive protein and white blood cell count. We determined the predictive value of pro-ADM and serum AA measurements in PPROM and PPROM with histological chorioamnionitis. We suggest using pro-ADM and serum AA biomarkers for detecting the histological chorioamnionitis at an earlier stage in PPROM without any clinical signs.
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http://dx.doi.org/10.1089/jir.2012.0134DOI Listing
November 2013

Parental knowledge and attitudes regarding the emergency treatment of avulsed permanent teeth.

Eur J Dent 2012 Oct;6(4):370-5

Department of Pediatric Dentistry, Faculty of Dentistry, Ondokuz Mayis University, Samsun, TURKIYE.

Objective: The aim of this study was to examine parental knowledge and attitudes about avulsed permanent teeth and their emergency treatment in children.

Methods: A total of 289 parents of children aged 6-12 years receiving care at the Ondokuz Mayis University Pediatric Dentistry Clinic were included in the study. Questionnaires were used to collect data on parents' demographic characteristics, previous training in traumatic dental injury and treatment and knowledge of avulsed permanent teeth and their emergency management. Chi-square tests were used to identify differences in responses for different variables.

Results: More than half of the participating parents were female (69.6%) and between the age of 30-39 years (64.4%). Most (90.7%) reported that they would not replant the avulsed tooth in its socket, and most (68.2%) gave correct answers regarding the optimum time for which an avulsed tooth should be left out of the mouth prior to replantation. However, most parents were not sufficiently informed about the appropriate method for cleaning a dirty avulsed tooth or transporting it to a dentist or doctor. None of the demographic characteristics or training status was found to have a statistically significant effect on the number of correct responses regarding appropriate management of avulsion injuries (P>.05)

Conclusion: This study revealed that the parental level of knowledge about the emergency treatment of avulsion in children is inadequate and that educational campaigns are necessary to improve proper emergency management of dental avulsion.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3474550PMC
October 2012

Use of a perichondrial autograft on the peritendinous adhesion: an experimental study in rabbits.

Acta Orthop Traumatol Turc 2012 ;46(3):208-14

Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey.

Objective: The aim of this experimental study was to evaluate the use of a clinically available perichondrium graft as an adjunct to surgical tendon repair.

Methods: Eight male New Zealand white rabbits of similar height and weight were used in this study. The left and right Achilles tendons were used as the experimental and control group, respectively. Perichondrium grafts were harvested from the right ears of the rabbits. Both Achilles tendons were clearly cut and repaired. After the repair, the perichondrium graft was wrapped around the tendon repair sites of the left Achilles tendons. Rabbits were sacrificed after six weeks and the tendons were examined macroscopically and histopathologically.

Results: Macroscopically and histopathologically, less adhesion occurred when the perichondrium graft was wrapped around the tendon repair site compared to the control group.

Conclusion: Perichondrium graft may isolate the repaired tendon and may reduce scar formation and adhesions during the healing period.
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http://dx.doi.org/10.3944/aott.2012.2668DOI Listing
October 2012

Enhancement of the activity and enantioselectivity of lipase by sol-gel encapsulation immobilization onto β-cyclodextrin-based polymer.

Appl Biochem Biotechnol 2012 Apr 1;166(8):1927-40. Epub 2012 Mar 1.

Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey.

Candida rugosa lipase was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of β-cyclodextrin-based polymer. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yield of the encapsulated lipase was 65 U/g, which is 7.5 times higher than that of the covalently immobilized lipase. The β-cyclodextrin-based encapsulated lipases had higher conversion and enantioselectivity compared with covalently immobilized lipase. The study confirms an excellent enantioselectivity (E >300) for the encapsulated lipase with an enantiomeric excess value of 98% for S-naproxen.
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http://dx.doi.org/10.1007/s12010-012-9621-zDOI Listing
April 2012