Dr. Elenice Deffune, PhD - Botucatu Medical School - Professor

Dr. Elenice Deffune

PhD

Botucatu Medical School

Professor

Botucatu, São Paulo | Brazil

Main Specialties: Allergy & Immunology, Biology, Biotechnology, Blood Banking - Transfusion Medicine, Hematology & Oncology

Additional Specialties: Cellular Therapy/Tissue Engineering/Clinical Transfusion

ORCID logohttps://orcid.org/0000-0002-0533-3248


Top Author

Dr. Elenice Deffune, PhD - Botucatu Medical School - Professor

Dr. Elenice Deffune

PhD

Introduction

Elenice Deffune completed her Ph.D. in Immunology - University of Paris VI (Pierre et Marie Curie) in 1992. She is currently an Assistant Professor at the Paulista State University Júlio de Mesquita Filho in the course of Hemotherapy. It is responsible for the Cell Engineering Laboratory at the Botucatu Hemocenter having 4 active research lines: 1) production of murine monoclonal antibodies, 2) culture and differentiation of stem cells of different species, 3) cell therapy and 4) Bioactive Cure Production Biocuratives). He has published 50 articles in specialized journals and 77 papers in annals of events. It has 24 books published. It has 15 technological products and 3 processes or techniques. Participated in 5 events abroad and 129 in Brazil. He supervised 28 master's theses and co-supervised 4, co-supervised 4 doctoral theses, besides having guided 21 scientific initiation and CBT work in the areas of Medicine, Nursing and Physiology. Received 10 awards and / or honors. Between 1992 and 2006 he coordinated 16 research projects. Currently participates in 7 research projects, and coordinates 6 of these. He works in the area of ??Medicine and Biomedicine. In his professional activities he interacted with 328 collaborators in co-authorship of scientific works. In his Lattes curriculum the most frequent terms in the contextualization of scientific, technological and artistic-cultural production are hemotherapy, biosafety, monoclonal antibodies, flow cytometry, stem cells, quality control, biological risk and blood donation.

Primary Affiliation: Botucatu Medical School - Botucatu, São Paulo , Brazil

Specialties:

Additional Specialties:


View Dr. Elenice Deffune’s Resume / CV

Education

Nov 2018
Biotechnology and Cellular Therapy
master
Jun 2018
Regenerative Medecine
high scholl
May 2018
Cellular Culture
high school

Experience

Jul 1992
Universidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatu
Docente da disciplina de Hemoterapia e Responsável pelo Laboratório de Engenharia Celular
Urologia

Publications

60Publications

1386Reads

14Profile Views

130PubMed Central Citations

Adipose-derived stem cells and adipose-derived stem cell-conditioned medium modulate in situ imbalance between collagen I- and collagen V-mediated IL-17 immune response recovering bleomycin pulmonary fibrosis.

Histol Histopathol 2020 Mar 18;35(3):289-301. Epub 2019 Jul 18.

Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.

The immunogenic collagen V (Col V) and the proinflammatory cytokine interleukin (IL)-17 have been implicated in the pathogenesis of multiple autoimmune diseases. Col V is also up-regulated during adipogenesis and can stimulate adipocyte differentiation in vitro. Conditioned medium (CM) generated from adipose-derived mesenchymal stem cells (MSCs) reduces bleomycin (BLM)-induced lung injury in rats, suggesting a crucial role in situ of immunomodulatory factors secreted by MSCs in these beneficial effects. In the present work, we investigated this hypothesis, analyzing levels of plasma inflammatory mediators and inflammatory and fibrotic mediators in the lung tissue of BLM-injured rats after treatment with MSCs and CM. Pulmonary fibrosis was intratracheally induced by BLM. After 10 days, BLM animals were further randomized into subgroups receiving saline, MSCs, or CM intravenously. On days 14 and 21, the animals were euthanized, and the lungs were examined through protein expression of nitric oxide synthase (NOS), IL-17, transforming growth factor-? (TGF-?), vascular endothelial growth factor, endothelin-1, and the immunogenic Col V through histological quantitative evaluation and plasma levels of fibrinogen, Von Willebrand factor, and platelet-derived growth factor (PDGF). Rats that had been injected with MSCs and CM showed a significant increase in weight and significant improvements at 14 and 21 days after intravenous injection at both time points of analysis of plasma fibrinogen, PDGF, and Von Willebrand factor and NOS-2 expression, supporting an early anti-inflammatory action, thus reducing TGF-? and collagen I fibers. In contrast, intravenous injection of CM was able to significantly increase the deposition of Col V fibers and IL-17 on both day 14 and day 21 as compared with the amount observed in rats from the BLM group and MSC groups. In conclusion, this study reinforces previous observations on the therapeutic properties of MSCs and CM and is the first report to demonstrate the association of its actions with immunomodulatory biomarkers on lung tissue. We concluded that adipose-derived stem cells and adipose-derived stem cells-CM modulate an in situ imbalance between collagen I- and Col V-mediated IL-17 immune response, emerging as a promising therapeutic option for recovering from BLM pulmonary fibrosis.

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http://dx.doi.org/10.14670/HH-18-152DOI Listing
March 2020
5 Reads
2.096 Impact Factor

Photodynamic inactivation for in vitro decontamination of Staphylococcus aureus in whole blood.

Photodiagnosis Photodyn Ther 2019 Dec 11;28:58-64. Epub 2019 Aug 11.

São Carlos Institute of Physics, University of São Paulo, PO Box 369, 13560-970, São Carlos, São Paulo, Brazil.

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http://dx.doi.org/10.1016/j.pdpdt.2019.08.013DOI Listing
December 2019
1 Read
2.014 Impact Factor

Immunosensor for the Diagnostics of Autoimmune Hemolytic Anemia (AIHA) Based on Immobilization of a Monoclonal Antibody on a Layer of Silk Fibroin.

J Nanosci Nanotechnol 2019 Jul;19(7):3772-3776

Universidade Estadual Paulista, Instituto de Química, Araraquara, SP 14800-060, Brazil.

The diagnostics of the autoimmune hemolytic anemia (AIHA), a rare disease caused by autoantibody-induced hemolysis, is still prone to false positives for it is based on visual observation in the so-called Direct Coombs test. In this study, we developed a specific IgG hemolysis immunosensor produced with layer-by-layer (LbL) films containing a monoclonal antibody against human immunoglobulin (mAbIMUG) deposited along with a layer of silk fibroin (SF) derived from cocoons. Adsorption of mAbIMUG on a SF layer was confirmed by the fluorescence emission band at 326 nm. Immunosensors were prepared with LbL films deposited on interdigitated gold electrodes for impedance spectroscopy and on screen printed carbon electrodes for electrochemical measurements. When the SF/mAbIMUGLbL film was exposed to healthy red blood cells (RBCs), no cell binding was observed by the optical microscopy images. In addition, no major changes were observed in the signals of the square wave voltammogram and in the impedance spectra. In contrast, the electrochemical signal was significantly increased and the dielectric loss curve shifted for the sensing units containing RBCs with the antibody attached on the surface (""). Furthermore, cell attachment was so strong that optical images still showed covered electrodes even after washing in PBS buffer. The detection with two distinct methods seems promising for an effective diagnosis of AIHA.

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http://dx.doi.org/10.1166/jnn.2019.16305DOI Listing
July 2019
8 Reads
1.556 Impact Factor

Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.

Res Vet Sci 2019 Jun 25;124:38-45. Epub 2019 Feb 25.

Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil. Electronic address:

Osteoarthritis is an incapacitating disease characterized by pain and a progressive decrease in joint mobility. The implantation of mesenchymal stem cells (MSCs) has shown promising results for its treatment. The challenge remains to keep the cells longer at the site of action, increasing their therapeutic potential. The aim of this study was to evaluate the effectiveness of the Qtracker® 655 nanocrystal marking on allogeneic synovial membrane (SM) MSCs, encapsulated in alginate hydrogel, evaluating the migration of these cells. The 10 radiocarpal joints were submitted to arthroscopic surgery (D0), divided into two groups. The chondral defect was treated according to the group: GA free-labelled MSC and GB labelled MSC microcapsules. Seven days after lesion induction and implantation of labelled cells, biopsies of the lesion site were performed in two animals, and fragments of SM and joint capsule also collected, which were frozen and later processed for fluorescence microscopy. The synovial fluid of the three animals was analyzed by flow cytometry three times - 3, 7 and 21?days after application. The cellular marking with the nanocrystals allowed the visualization of the cells in cartilage, synovial membrane, synovial fluid and articular capsule, but with a predilection for the synovial membrane and the lesion site was scarce. The labelled MSC in microcapsules were scarce in the synovial fluid and could be related to the small quantity of MSCs leaving the pores of the microcapsules, also favorable results, as the cells release paracrine effects acting for a long period until the cellular differentiation.

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http://dx.doi.org/10.1016/j.rvsc.2019.02.005DOI Listing
June 2019
21 Reads
1.409 Impact Factor

Applicability of papain solutions in immunohematology.

Einstein (Sao Paulo) 2019 May;17(2):eAO4328

Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, SP, Brazil.

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http://dx.doi.org/10.31744/einstein_journal/2019AO4328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497125PMC
May 2019
5 Reads

Explanation for the signs and symptoms of tooth eruption: mast cells.

Dental Press J Orthod 2019 May;24(2):20-31

Universidade de São Paulo, Faculdade de Odontologia de Bauru (Bauru/SP, Brazil).

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http://dx.doi.org/10.1590/2177-6709.24.2.020-031.oinDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526767PMC
May 2019
2 Reads

Sonic hedgehog drives layered double hydroxides-induced acute inflammatory landscape.

Colloids Surf B Biointerfaces 2019 Feb 22;174:467-475. Epub 2018 Nov 22.

Lab. de Bioensaios e Dinâmica Celular, Departamento de Química e Bioquímica, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus Botucatu, São Paulo, CEP 18618-970, Brazil. Electronic address:

Although layered double hydroxides (LDH) have been listed as promising nanomaterials in human healthcare, very little has been achieved on osteoblast inflammatory signaling. Thus, osteoblasts were challenged with two LDHs (MgAl-Cl and ZnAl-Cl, at 0.002?mg/mL) up to 24?h, establishing an acute inflammatory mechanism, as well as identifying whether Sonic hedgehog (Shh) signaling has an influence. Functional experiments were performed by previously treating (2?h) semiconfluent osteoblast cultures with cyclopamine molecule (cyc), a widely used Shh inhibitor. Considering inflammasome complex, the asc1 gene was significantly up-expressed in response to ZnAl-Cl - LDHs, as well as the nrlp3 gene. By treating the osteoblast with cyc, the asc1 gene presented an even higher profile. Our results found a down-modulation of major pro-inflammatory cytokines-related genes, when tnf? and il1ß were significantly down-modulated in response to LDHs. Conversely, anti-inflammatory cytokines were up-modulated considering the same experimental procedures. Except the il6, the other il13, il10, and tgfß genes were up modulated. Additionally, Shh signaling seems to modulate this repertory as both the il13 and il10 genes were significantly up-modulated when the Shh signaling was inhibited. Altogether, our results reveal for the first time the exigency of Shh-dependent anti-inflammatory signals in LDH-induced osteoblast responses.

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http://dx.doi.org/10.1016/j.colsurfb.2018.11.051DOI Listing
February 2019
5 Reads
4.152 Impact Factor

Canine Adipose-Derived Mesenchymal Stromal Cells Enhance Neuroregeneration in a Rat Model of Sciatic Nerve Crush Injury.

Cell Transplant 2019 01 28;28(1):47-54. Epub 2018 Oct 28.

1 Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), São Paulo, Brazil.

Crush injuries in peripheral nerves are frequent and induce long-term disability with motor and sensory deficits. Due to axonal and myelin sheath disruptions, strategies for optimized axonal regeneration are needed. Multipotent mesenchymal stromal cells (MSC) are promising because of their anti-inflammatory properties and secretion of neurotrophins. The present study investigated the effect of canine adipose tissue MSC (Ad-MSC) transplantation in an experimental sciatic nerve crush injury. Wistar rats were divided into three groups: sham ( n = 8); Crush+PBS ( n = 8); Crush+MSC ( n = 8). Measurements of sciatic nerve functional index (SFI), muscle mass, and electromyography (EMG) were performed. Canine Ad-MSC showed mesodermal characteristics (CD34-, CD45-, CD44+, CD90+ and CD105+) and multipotentiality due to chondrogenic, adipogenic, and osteogenic differentiation. SFI during weeks 3 and 4 was significantly higher in the Crush+MSC group ( p < 0.001). During week 4, the EMG latency in the Crush+MSC groups had better near normality ( p < 0.05). The EMG amplitude showed results close to normality during week 4 in the Crush+MSC group ( p < 0.04). There were no statistical differences in muscle weight between the groups ( p > 0.05), but there was a tendency toward weight gain in the Crush+MSC groups. Better motor functional recovery after crush and perineural canine Ad-MSC transplantation was observed during week 2. This was maintained till week 4. In conclusion, the canine Ad-MSC transplantation showed early pro-regenerative effects between 2-4 weeks in the rat model of sciatic nerve crush injury.

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http://dx.doi.org/10.1177/0963689718809045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322136PMC
January 2019
13 Reads
3.570 Impact Factor

Correction to: Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules.

Stem Cell Res Ther 2018 10 7;9(1):259. Epub 2018 Oct 7.

Department of Veterinary Surgery and Anesthesiology, University of Veterinary Medicine and Animal Science UNESP, District of Rubião Júnior, s / n, Botucatu, São Paulo, Brazil.

The original article [1] contained a minor error regarding the mean diameter of the alginate microcapsules described in relation to Fig. 4 in the Results section. The microcapsules had an actual mean diameter of 3000 ?m instead of 1000 ?m as mistakenly mentioned in the original article.

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https://stemcellres.biomedcentral.com/articles/10.1186/s1328
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http://dx.doi.org/10.1186/s13287-018-0999-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174064PMC
October 2018
4 Reads
3.368 Impact Factor

Bisphenol a and mesenchymal stem cells: Recent insights.

Life Sci 2018 08 18;206:22-28. Epub 2018 May 18.

Department of Morphology, Institute of Biosciences, Univ Estadual Paulista - UNESP, Botucatu, Sao Paulo, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.lfs.2018.05.023DOI Listing
August 2018
3 Reads
2.702 Impact Factor

Preeclamptic plasma stimulates the expression of miRNAs, leading to a decrease in endothelin-1 production in endothelial cells.

Pregnancy Hypertens 2018 Apr 27;12:75-81. Epub 2018 Mar 27.

Department of Pharmacology, Institute of Biosciences of Botucatu, São Paulo State University (UNESP), Botucatu, SP, Brazil. Electronic address:

Preeclampsia is a major cause of maternal and fetal morbidity and mortality worldwide. It is a multisystem pregnancy syndrome characterized by general endothelial dysfunction caused mainly by plasma factors and debris in endothelial cells. It is widely accepted that endothelin-1 (ET-1) is involved in the pathophysiology of preeclampsia, and so it is of interest to ascertain whether the ET-1 gene (EDN1) can be targeted with tools such as miRNAs. Therefore, we investigated the relationship between the expression of miRNAs that putatively target EDN1 (and so affect ET-1 levels) in HUVECs incubated with plasma from preeclamptic women. EDN1 expression and ET-1 levels in HUVECs incubated with plasma from women with preeclampsia were similar to those in plasma from healthy pregnant women. Expression of miRNAs let-7a, -7b, and -7c, and to a lesser degree 125a and 125b, was increased in preeclampsia. Expression of miRNAs of the let-7 family was significantly negatively correlated with ET-1 levels in preeclampsia. Transfection of the preeclampsia cultures with mimic miRNA let-7 decreased ET-1 levels. Our findings show that preeclamptic plasma stimulates the expression of miRNAs in HUVECs, leading to a decrease in ET-1levels, which suggests that therapeutic miRNAs may aid in the management of preeclampsia.

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http://dx.doi.org/10.1016/j.preghy.2018.03.001DOI Listing
April 2018
6 Reads

Multiple Tolerization Reduces Antibody Binding Against Tolerogen Cells: Implications for the Production of Monoclonal Antibodies.

Monoclon Antib Immunodiagn Immunother 2018 Apr;37(2):100-104

1 Monoclonal Antibody Laboratory, Proteomics Center , Univ Estadual Paulista-UNESP, School of Pharmaceutical Sciences, Araraquara, Brazil .

We report an immunization technique that can update the production of monoclonal antibodies (mAbs): the multiple tolerization subtractive immunization (MTSI). A total of 10 BALB/C mice were used. Animals in group 1 received one inoculation of RWPE-1 cells (nontumoral), followed by cyclophosphamide, and then received serial inoculations of nonirradiated PC3 cells (tumoral). Animals in group 2 received our MTSI protocol, as follows: one inoculation of RWPE-1 cells, followed by cyclophosphamide (Cy). This whole tolerization step was repeated three other times, with 14-day intervals between the last Cy exposure and the next RWPE-1 cell inoculation. Finally, the animals received the same nonirradiated PC3 cell exposure as group 1. Blood was taken from each animal, and their polyclonal sera individually tested against the nontumoral RWPE-1 cells in flow cytometry. We found out that, after the MTSI was employed, the serum of the immunized animals, in group 2, contained considerably less antibodies that reacted against the tolerogenic cells, compared with the serum of the animals that underwent regular subtractive immunization. We showed that, by repeating the tolerization cycles, the polyclonal antibodies produced by mice have a reduced specificity toward common/immunodominant epitopes present at nontumoral cells, and thus this technique can be readily used by others in studies involving murine mAb protocols.

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http://dx.doi.org/10.1089/mab.2017.0055DOI Listing
April 2018
6 Reads

Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules.

BMC Vet Res 2018 Mar 27;14(1):114. Epub 2018 Mar 27.

Department of Veterinary Surgery and Anesthesiology, University of Veterinary Medicine and Animal Science UNESP, District of Rubião Júnior, s / n, Botucatu, São Paulo, Brazil.

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http://dx.doi.org/10.1186/s12917-018-1425-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870504PMC
March 2018
22 Reads
1.777 Impact Factor

Bleeding Risk with Long-Term Low-Dose Aspirin: A Systematic Review of Observational Studies.

PLoS One 2016 4;11(8):e0160046. Epub 2016 Aug 4.

University of Zaragoza, University Clinic Hospital, IIS Aragón, CIBERehd, Zaragoza, Spain.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0160046PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4973997PMC
August 2017
84 Reads
7 Citations
3.234 Impact Factor

Parenting a child with metabolic diseases: Impact on health related quality of life of parents.

Diabetes Metab Syndr 2017 Jan - Mar;11(1):25-29. Epub 2016 Jul 25.

College of Nursing, Sultan Qaboos University, Oman. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S18714021163011
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http://dx.doi.org/10.1016/j.dsx.2016.07.002DOI Listing
March 2017
14 Reads

Toxicity of squamocin on Aedes aegypti larvae, its predators and human cells.

Pest Manag Sci 2017 Mar 4;73(3):636-640. Epub 2016 Aug 4.

Laboratory of Ultrastructural Cell, Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.

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http://dx.doi.org/10.1002/ps.4350DOI Listing
March 2017
18 Reads
2.694 Impact Factor

Postoperative pain control in cats: clinical trials with pre-emptive lidocaine epidural co-administered with morphine or methadone.

J Feline Med Surg 2016 11 10;18(11):882-888. Epub 2016 Jul 10.

3 Department of Veterinary Medicine, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.

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http://dx.doi.org/10.1177/1098612X15602738DOI Listing
November 2016
34 Reads
1.160 Impact Factor

Comparison of lidocaine, levobupivacaine or ropivacaine for distal paravertebral thoracolumbar anesthesia in ewes.

Vet Anaesth Analg 2016 Nov 22;43(6):670-674. Epub 2016 Feb 22.

Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.

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http://dx.doi.org/10.1111/vaa.12353DOI Listing
November 2016
15 Reads
1 Citation
1.720 Impact Factor

Kostmann syndrome: oral aspects and 10-year follow-up case report.

Spec Care Dentist 2016 Nov 3;36(6):339-344. Epub 2016 Aug 3.

Head and Full Professor, Special Care Dentistry Center, Dental School, University of São Paulo, São Paulo, Brazil.

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http://dx.doi.org/10.1111/scd.12192DOI Listing
November 2016
7 Reads

Histologic characterization of regenerated tissues after pulp revascularization of immature dog teeth with apical periodontitis using tri-antibiotic paste and platelet-rich plasma.

Arch Oral Biol 2016 Nov 26;71:122-128. Epub 2016 Jul 26.

Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain. Electronic address:

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http://dx.doi.org/10.1016/j.archoralbio.2016.07.007DOI Listing
November 2016
32 Reads
3 Citations
1.880 Impact Factor

Cancer risk in siblings of children with congenital malformations.

Cancer Epidemiol 2016 10 1;44:59-64. Epub 2016 Aug 1.

Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark; Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.

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http://dx.doi.org/10.1016/j.canep.2016.07.011DOI Listing
October 2016
7 Reads
1 Citation
2.560 Impact Factor

Lentinan protects pancreatic β cells from STZ-induced damage.

J Cell Mol Med 2016 10 22;20(10):1803-12. Epub 2016 Jul 22.

Department of Biochemistry and Molecular Biology, Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu, China.

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http://dx.doi.org/10.1111/jcmm.12865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020630PMC
October 2016
36 Reads
6 Citations

C-Type Natriuretic Peptide Induces Anti-contractile Effect Dependent on Nitric Oxide, Oxidative Stress, and NPR-B Activation in Sepsis.

Front Physiol 2016 23;7:226. Epub 2016 Jun 23.

Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo Ribeirão Preto, Brazil.

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http://dx.doi.org/10.3389/fphys.2016.00226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917550PMC
July 2016
25 Reads

Yacon (Smallanthus sonchifolius) as a Food Supplement: Health-Promoting Benefits of Fructooligosaccharides.

Nutrients 2016 Jul 21;8(7). Epub 2016 Jul 21.

Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu 18618-689, Brazil.

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http://dx.doi.org/10.3390/nu8070436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963912PMC
July 2016
54 Reads
2 Citations
3.150 Impact Factor

Antihypertensive therapy in pre-eclampsia: effects of plasma from nonresponsive patients on endothelial gene expression.

Pharmacogenomics 2016 07 27;17(10):1121-1127. Epub 2016 Jun 27.

Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, Brazil.

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http://dx.doi.org/10.2217/pgs-2016-0033DOI Listing
July 2016
22 Reads
1 Citation
3.220 Impact Factor

4-Aminoantipyrine reduces toxic and genotoxic effects of doxorubicin, cisplatin, and cyclophosphamide in male mice.

Mutat Res Genet Toxicol Environ Mutagen 2016 Jul 20;805:19-24. Epub 2016 May 20.

Center for Stem Cells, Cell Therapy and Genetic Toxicology Studies, Maria Aparecida Pedrossian University Hospital, EBSERH, Campo Grande, Mato Grosso do Sul, Brazil; Postgraduate Program in Pharmacy, Center for Biological and Health Sciences, UFMS, Campo Grande, Mato Grosso do Sul, Brazil; Postgraduate Program in Health and Development in the Midwestern Region, Medical School, UFMS, Campo Grande, Mato Grosso do Sul, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.mrgentox.2016.05.009DOI Listing
July 2016
27 Reads
1 Citation
2.420 Impact Factor

Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells.

J Cell Mol Med 2016 06 24;20(6):1198-200. Epub 2016 Mar 24.

Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.

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http://dx.doi.org/10.1111/jcmm.12767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882987PMC
June 2016
15 Reads
3 Citations

Mesenchymal stem cell therapy promotes the improvement and recovery of renal function in a preclinical model.

Genet Mol Biol 2016 Jun;39(2):290-9

Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário "Maria Aparecida Pedrossian", Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brazil.

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http://dx.doi.org/10.1590/1678-4685-GMB-2015-0178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910560PMC
June 2016
27 Reads
1 Citation
1.202 Impact Factor

Cardanol: toxicogenetic assessment and its effects when combined with cyclophosphamide.

Genet Mol Biol 2016 Apr-Jun;39(2):279-89

Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta", Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.

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http://dx.doi.org/10.1590/1678-4685-GMB-2015-0170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910563PMC
June 2016
13 Reads
1.202 Impact Factor

Multiple Apical Periodontitis Influences Serum Levels of Cytokines and Nitric Oxide.

J Endod 2016 May 5;42(5):747-51. Epub 2016 Apr 5.

Department of Endodontics, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil.

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http://dx.doi.org/10.1016/j.joen.2016.01.022DOI Listing
May 2016
21 Reads
2 Citations
3.380 Impact Factor

Proteins involved on TGF-β pathway are up-regulated during the acute phase of experimental Chagas disease.

Immunobiology 2016 May 29;221(5):587-94. Epub 2016 Jan 29.

Laboratório de Genômica Funcional e Bioinformática-Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro RJ, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.imbio.2016.01.009DOI Listing
May 2016
32 Reads
5 Citations
3.044 Impact Factor

MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression in the prostatic tissue of two ethanol-preferring rat models.

Anal Cell Pathol (Amst) 2015 15;2015:954548. Epub 2015 Jul 15.

Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil.

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http://dx.doi.org/10.1155/2015/954548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518171PMC
March 2016
27 Reads
1.760 Impact Factor

Is Doppler ultrasound useful for evaluating gestational trophoblastic disease?

Clinics (Sao Paulo) 2015 Dec;70(12):810-5

Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.

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http://dx.doi.org/10.6061/clinics/2015(12)08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676324PMC
December 2015
13 Reads
1 Citation
1.422 Impact Factor

Preliminary analysis on the MD-4(®) plasma-sprayed titanium acetabular component.

Rev Bras Ortop 2015 Mar-Apr;50(2):206-13. Epub 2015 Mar 19.

Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.

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http://dx.doi.org/10.1016/j.rboe.2015.03.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519615PMC
August 2015
9 Reads

Platelet-rich plasma (PRP) applied during total knee arthroplasty.

Rev Bras Ortop 2015 Mar-Apr;50(2):186-94. Epub 2015 Mar 12.

Hemocenter of Botucatu, Faculdade de Medicina de Botucatu (UNESP), Botucatu, SP, Brazil.

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http://dx.doi.org/10.1016/j.rboe.2015.02.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519620PMC
August 2015
26 Reads
3 Citations

Platelet and plasma bioactive scaffolds for stem cell differentiation: what are we missing?

Platelets 2014 31;25(7):556-7. Epub 2013 Oct 31.

Cell Engineering Lab, Blood Transfusion Center, Botucatu Medical School, Univ Estadual Paulista , UNESP, Botucatu, SP , Brazil and.

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http://www.tandfonline.com/doi/full/10.3109/09537104.2013.83
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http://dx.doi.org/10.3109/09537104.2013.836748DOI Listing
June 2015
82 Reads
1 Citation
2.630 Impact Factor

Finasteride inhibits human prostate cancer cell invasion through MMP2 and MMP9 downregulation.

PLoS One 2013 30;8(12):e84757. Epub 2013 Dec 30.

Univ Estadual Paulista - UNESP, Institute of Biosciences, Department of Morphology, Extracellular Matrix Lab, Botucatu, São Paulo, Brazil.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084757PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875555PMC
February 2015
16 Reads
7 Citations
3.234 Impact Factor

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay.

Genet Mol Res 2015 Jan 23;14(1):339-48. Epub 2015 Jan 23.

Programa de Pós Graduação em Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.

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http://dx.doi.org/10.4238/2015.January.23.7DOI Listing
January 2015
14 Reads
3 Citations
0.780 Impact Factor

Role of fibroblast growth factor-23 in the pathogenesis of atherosclerosis in peritoneal dialysis patients.

Genet Mol Res 2015 Jan 30;14(1):719-29. Epub 2015 Jan 30.

Department of Nephrology, Second Affiliated Hospital of Soochow University, Suzhou, China

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http://dx.doi.org/10.4238/2015.January.30.15DOI Listing
January 2015
8 Reads
2 Citations
0.780 Impact Factor

Treatment of osteochondral injuries with platelet gel.

Clinics (Sao Paulo) 2014 Dec;69(10):694-8

Faculty of Medicine of Botucatu, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, SP, Brazil.

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http://dx.doi.org/10.6061/clinics/2014(10)08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221310PMC
December 2014
25 Reads
1.422 Impact Factor

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds.

Exp Cell Res 2014 Aug 12;326(1):103-11. Epub 2014 Jun 12.

Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil; Department of Urology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.

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http://dx.doi.org/10.1016/j.yexcr.2014.05.023DOI Listing
August 2014
52 Reads
1 Citation
3.250 Impact Factor

Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors.

J Vasc Surg 2014 Jun 2;59(6):1677-85. Epub 2013 Jul 2.

Cell Engineering Laboratory, Blood Transfusion Center, Botucatu Medical School, UNESP-Paulista State University, Botucatu, Brazil; Department of Urology, Botucatu Medical School, UNESP-Paulista State University, Botucatu, Brazil.

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http://dx.doi.org/10.1016/j.jvs.2013.05.032DOI Listing
June 2014
98 Reads
5 Citations
3.021 Impact Factor

Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro.

Arch Oral Biol 2014 Mar 7;59(3):268-76. Epub 2013 Dec 7.

Department of Basic Sciences, School of Dentistry, UNESP - Univ. Estadual Paulista, São Paulo, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.archoralbio.2013.11.017DOI Listing
March 2014
87 Reads
3 Citations
1.880 Impact Factor

How to improve your PubMed/MEDLINE searches: 1. background and basic searching.

J Telemed Telecare 2013 Dec 6;19(8):479-86. Epub 2013 Nov 6.

School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1177/1357633X13512061DOI Listing
December 2013
25 Reads
1 Citation
1.740 Impact Factor

Platelet-rich plasma and chronic wounds: remaining fibronectin may influence matrix remodeling and regeneration success.

Cytotherapy 2013 Nov 30;15(11):1436-9. Epub 2013 Aug 30.

Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, Universidade Estadual Paulista-UNESP, Botucatu, SP, Brazil; Department of Morphology, Extracellular Matrix Laboratory, Botucatu Biosciences Institute, Universidade Estadual Paulista-UNESP, Botucatu, SP, Brazil. Electronic address:

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http://dx.doi.org/10.1016/j.jcyt.2013.05.019DOI Listing
November 2013
12 Reads
4 Citations
3.293 Impact Factor

Platelet lysate 3D scaffold supports mesenchymal stem cell chondrogenesis: an improved approach in cartilage tissue engineering.

Platelets 2013 30;24(3):219-25. Epub 2012 May 30.

Extracellular Matrix Laboratory, Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista-UNESP, District of Rubião Júnior S/N, 18618-970, Botucatu, SP, Brazil.

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http://dx.doi.org/10.3109/09537104.2012.686255DOI Listing
September 2013
56 Reads
6 Citations
2.630 Impact Factor

Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.

Carvalho Ade M, Badial PR, Álvarez LE, Yamada AL, Borges AS, Deffune E, Hussni CA, Garcia Alves AL, Stem cell research & therapy, 2013, vol. 4, no. 4, pp. 85

http://europepmc.org/abstract/med/23876512

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July 2013
12 Reads

Organosilylated complex [Eu(TTA)3(Bpy-Si)]: a bifunctional moiety for the engeneering of luminescent silica-based nanoparticles for bioimaging.

Langmuir 2013 May 3;29(19):5878-88. Epub 2013 May 3.

Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux, Université de Toulouse, UPS-CNRS 5085, Toulouse, France.

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http://dx.doi.org/10.1021/la400365cDOI Listing
May 2013
33 Reads
1 Citation
4.460 Impact Factor

The pernicious cycle of VLUs in Brazil: epidemiology, pathogeny and auxiliary healing methods.

J Wound Care 2013 Apr;22(4):186-8, 190, 192-3

Pontifical University of Campinas, Sao Paulo, Brazil.

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http://dx.doi.org/10.12968/jowc.2013.22.4.186DOI Listing
April 2013
1.110 Impact Factor

Fibronectin induces MMP2 expression in human prostate cancer cells.

Biochem Biophys Res Commun 2013 Jan 19;430(4):1319-21. Epub 2012 Dec 19.

Univ Estadual Paulista - UNESP, Institute of Biosciences, Department of Morphology, Botucatu, SP, Brazil.

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http://dx.doi.org/10.1016/j.bbrc.2012.12.031DOI Listing
January 2013
19 Reads
17 Citations
2.300 Impact Factor

Periodontal tissue engineering after tooth replantation.

J Periodontol 2011 May 8;82(5):758-66. Epub 2010 Nov 8.

School of Dentistry, Federal University of Pará, UFPA, Pará, Brazil.

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http://dx.doi.org/10.1902/jop.2010.100448DOI Listing
May 2011
28 Reads
3 Citations
2.710 Impact Factor

Taenia saginata: production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens.

Exp Parasitol 2010 Dec 11;126(4):621-5. Epub 2010 Jun 11.

UNESP, Univ. Estadual Paulista, Campus Botucatu, Graduation Program on Veterinary Medicine, School of Veterinary Medicine and Zootechny, Botucatu, SP, Brazil.

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http://dx.doi.org/10.1016/j.exppara.2010.06.010DOI Listing
December 2010
12 Reads
1.860 Impact Factor

Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.

Vet Immunol Immunopathol 2009 Dec 30;132(2-4):303-6. Epub 2009 Jun 30.

Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil.

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http://dx.doi.org/10.1016/j.vetimm.2009.06.014DOI Listing
December 2009
14 Reads
20 Citations
1.535 Impact Factor

Remifentanil, isoflurane, and preconditioning attenuate renal ischemia/reperfusion injury in rats.

Transplant Proc. 2009 Dec;41(10):4080-2. doi: 10.1016/j.transproceed.2009.09.078.

Transplant Proc

Abstract BACKGROUND: The purpose of this investigation was to examine the effect of isoflurane, remifentanil, and preconditioning in renal ischemia/reperfusion injury (IRI). METHODS: All 52 male Wistar rats were anesthetized with isoflurane, intubated and mechanically ventilated. The animals were randomly divided into: S group (sham; n = 11) that underwent only right nephrectomy; as well as the I group of right nephrectomy and ischemia for 45 minutes by clamping of left renal artery. (n = 11); the IP (n = 9), the R (n = 10), and the RP (n = 11) groups. In addition, the R and RP animals received remifentanil (2 microg.kg(-1).min(-1)) during the entire experiment. The IP and RP group underwent ischemic preconditioning (IPC = three cycles of 5 minutes). Serum creatinine values were determined before and after IRI, as well as 24 hours later. In addition to an Histological study, cells from the left kidney were evaluated for apoptosis by flow cytometry (FCM). RESULTS: The Creatinine value of 0.8 +/- 0.2 mg/dl in the S group was significantly lower at 24 hours than the I 3.9 +/- 1.5 mg/dl; IP 2.6 +/- 1.7 mg/dl; R 3.3 +/- 2.8 mg/dl; or RP 1.8 +/- 0.5 mg/dl groups. The RP group value was significantly lower than those of the I, IP, and R groups (p < 0.05). The S group showed less proximal tubular cell damage than the I, IP, R, and RP groups (p < 0.05). The percentages of apoptotic cells (FITC(+)/PI(-)) were: S group = 11.6 +/- 6.5; I = 16.7 +/- 7.3; IP = 37.0 +/- 28.4; R = 11.7 +/- 6.6, and RP = 8.8 +/- 1.5. The difference between the IP vs RP group was significant. Similar percentages of necrotic cells (FITC(+)/PI(+)) and intact cells (FITC(-)/PI(-)) were observed among the groups. CONCLUSIONS: Ischemic preconditioning showed no protective effect in the isoflurane group (IP) but when isoflurane was administered associated with remifentanil (RP), there was a beneficial effect on the kidney, as demonstrated by flow cytometry and serum creatinine values.

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December 2009
11 Reads

Top co-authors

Andrei Moroz
Andrei Moroz

Botucatu Medical School

12
Sergio Luis Felisbino
Sergio Luis Felisbino

São Paulo State University (UNESP)

4
Sergio L Felisbino
Sergio L Felisbino

Institute of Biosciences

4
Carlos Alberto Hussni
Carlos Alberto Hussni

School of Veterinary Medicine and Animal Science

3
Rodrigo Juliano Oliveira
Rodrigo Juliano Oliveira

Centro Universitário Filadélfia (UniFil)

3
Matheus Bertanha
Matheus Bertanha

School of Medicine

3
Ricardo C Cavalli
Ricardo C Cavalli

University of Sao Paulo

3