Publications by authors named "Elena G Lobanova"

3 Publications

  • Page 1 of 1

Novel Dimethylacetamide-Containing Formulation Improves Infraorbital Anaesthesia Efficacy in Rats with Periodontitis.

Adv Pharmacol Pharm Sci 2020 14;2020:3058735. Epub 2020 Apr 14.

Department of Operative Surgery and Clinical Anatomy, Department of Pathology, Department of Dentistry, Sechenov University, Moscow 119991, Russia.

Background: To evaluate acute toxicity and local anaesthetic activity of a formulation containing a novel dimethylacetamide derivative, antioxidant, and vasoconstrictor in rats with chronic periodontitis.

Methods: Novel anaesthetic dimethylacetamide-containing formulation LHT-15-32 was studied as 2% water solution. Its acute intravenous and subcutaneous toxicity was determined in mice. Pain sensitivity threshold of the upper second molar was determined in rats with experimental periodontitis. Oxidative stress activity and total antioxidant capacity were determined in rats' gingival mucosa by induced chemiluminescence. Local changes were evaluated in periodontal tissue by morphological examination. Tissue IL-1, IL-10, and TNF-α concentration was quantitatively assessed by an enzyme-linked immunosorbent assay. LHT-15-31 Na-blocking activity was studied on isolated neurons of ' parapharyngeal ganglion. Isolated sciatic nerve of was perfused with different concentrations of LHT-15-32 to assess its conductivity. Statistical analysis was used, and continuous variables were presented as mean ± square deviation. The normality of distribution was determined using ANOVA. Newman-Keuls parametric criterion was used for intergroup comparison. LD indexes were calculated by probit analysis.

Results: LHT-15-32 acute intravenous and subcutaneous toxicity was lower than that of its active substance. The formulation by infraorbital administration induced deep dental anaesthesia which lasted over 70 min and activated the local antioxidant defense system and decreased IL-1 level in gingival tissue. LHT-15-32 triggered tissue reparation around the impacted upper molar in rats assessed five days after administration. At 10 to 10 M concentration, LHT-15-32 inhibited sciatic nerve conductivity and blocked Na channels of isolated neurons in a dose-dependent manner.

Conclusions: The formulation may be considered as an effective and safe approach to anaesthetize upper molars with periodontitis.
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April 2020

The Mechanisms of the Regulation of Immune Response in Patients with Comorbidity of Chronic Obstructive Pulmonary Disease and Asthma.

Can Respir J 2016;2016:4503267. Epub 2016 Aug 31.

Institute of Medical Climatology and Rehabilitation Treatment, Vladivostok Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration, 73g Russkaya St., Vladivostok 690105, Russia.

. Comorbidity of chronic obstructive pulmonary disease (COPD) and asthma (asthma COPD overlap syndrome, ACOS) is a significant problem in pulmonary practice, whose pathogenetic issues are not clarified yet. . To study the features of the regulation of immune response in patients with comorbid COPD and asthma. . We assessed the levels of CD3, CD4, CD8, CD4/CD8, CD19, CD25, HLA-DR, total IgE, TNF-, IL-4, IFN-, TXB, and LTB in patients with comorbid COPD and asthma. . The study involved 44 people with COPD, 39 people with asthma, and 12 people with comorbid COPD and asthma. The specific features in comorbid COPD and asthma were lymphocytosis, increased absolute count of T-helper cells, increased cytotoxic T-lymphocytes in relative and absolute count, increased relative and absolute numbers of B-lymphocytes, and high levels of total IgE. The elevated levels of TNF- and IL-4 and inhibition of IFN- production were detected. The content of LTB was maximal; TXB levels were higher than in control group but lower than in COPD and asthma. . In comorbid COPD and asthma inflammation increased even during stable period. High levels of eicosanoids, low production of Th1-type cytokines, and active synthesis of opposition IL-4, along with increased IgE, indicate the activation of Th2-type immune response.
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August 2016

Composition of fatty acids in plasma and erythrocytes and eicosanoids level in patients with metabolic syndrome.

Lipids Health Dis 2011 May 19;10:82. Epub 2011 May 19.

Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration of SB RAMN-Institute of Medical Climatology and Rehabilitative Treatment, Russia.

Background: Disturbances of the fatty acids composition in plasma and red blood cells and eicosanoid synthesis play an important role in the metabolic syndrome (MS) formation.

Methods: The observation group included 61 people with metabolic syndrome (30 patients with MS and normal levels of insulin, 31 people with MS and insulin resistance--IR). The parameters of carbohydrate and lipid metabolism in blood serum were examined. The composition of nonesterified fatty acids (NEFA), fatty acid (FA) of red blood cells lipids was analyzed by gas-liquid chromatography. Eicosanoids level in MS patients blood serum was studied by enzyme immunoassay.

Results: In MS patients in the absence of glucose-insulin homeostasis disturbances and in patients with IR the accumulation of polyunsaturated fatty acids (18:2 n6, 18:3 n3, 22:4 n6) and lower pool of saturated FA (12:0, 14:0, 16: 0, 17:0) in plasma were discovered. A deficit of polyunsaturated FA (18:3 n3, 20:4 n6) with a predominance of on-saturated FA (14:0, 18:0) in erythrocyte membranes was revealed. In MS patients regardless of the carbohydrate metabolism status high levels of leukotriene B4 and 6-keto-prostaglandin-F1α in serum were found. The development of IR in MS patients leads to increased synthesis of thromboxane A2.

Conclusion: The results revealed a disturbance in nonesterified fatty acids of plasma lipids and red blood cells, eicosanoid synthesis in MS patients. The breach of the plasma and cell membranes fatty acids compositions, synthesis of vasoactive and proinflammatory eicosanoids is an important pathogenetic part of the MS development.
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May 2011