Publications by authors named "Ehsan Ghasemi"

9 Publications

  • Page 1 of 1

Antibiotic resistance, biofilm formation, and biofilm-associated genes among Stenotrophomonas maltophilia clinical isolates.

BMC Res Notes 2021 Apr 20;14(1):151. Epub 2021 Apr 20.

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objective: The purpose of the present study was to investigate the antimicrobial susceptibility pattern, biofilm production, and the presence of biofilm genes among the S. maltophilia clinical isolates. A total of 85 clinical isolates of S. maltophilia were collected from patients referred to several hospitals. Susceptibility to antibiotics was investigated by disc diffusion method according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). By the crystal violet staining method, the capability of biofilm formation was examined. The genes associated with biofilm production were investigated by the PCR-sequencing techniques.

Results: All isolates were resistant to doripenem, imipenem, and meropenem. Minocycline, trimethoprim/sulfamethoxazole and levofloxacin exhibited the highest susceptibility of 100%, 97.65%, and 95.29%, respectively. The results of crystal violet staining assay showed that all isolates (100%) form biofilm. Moreover, 24 (28.23%), 32 (37.65%), and 29 (34.12%) of isolates were categorized as weak, moderate, and strong biofilm producers, respectively. Biofilm genes including rpfF, spgM and rmlA had an overall prevalence of 89.41% (76/85), 100% (85/85) and 84.71% (72/85), respectively. Rational prescribing of antibiotics and implementation of infection control protocols are necessary to prevent further infection and development of antimicrobial resistance. Combination strategies based on the appropriate antibiotics along with anti-biofilm agents can also be selected to eliminate biofilm-associated infections.
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April 2021

A sectional complete denture for microstomic patients.

Dent Res J (Isfahan) 2020 Mar-Apr;17(2):162-166. Epub 2020 Mar 17.

Dental Material Research Center and Department of Prosthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

Microstomic patients may experience a significant limitation in the mandibular opening. The prosthetic rehabilitation of microstomic patients presents difficulties at all stages, from preliminary impressions to prosthetic fabrication. This clinical report described the method of fabrication of sectional denture for patients with microstomia caused by burns. The denture was fabricated in two pieces, and patients inserted and removed the denture easily.
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March 2020

The effect of functional stretching exercises on functional outcomes in spastic stroke patients: A randomized controlled clinical trial.

J Bodyw Mov Ther 2018 Oct 28;22(4):1004-1012. Epub 2017 Sep 28.

Department of Neurology& Isfahan Neurosciences Research Center, Faculty of Medical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Stroke is the biggest cause of disability in adults. Spasticity is a primary impairment of stroke with a highly variable prevalence. In the present research, we aimed to determine the impact of functional stretching exercises on functional outcomes in stroke patients.

Methods: Thirty stroke patients were randomized into two groups-Experimental group and control group for the purposes of the study. The subjects in the experimental group participated in a functional stretching training program at the rehabilitation center thrice a week for four weeks. The subjects in both groups were evaluated in 3 intervals, once at baseline, once at the end of the program, and once at 2 months following the program. Clinical assessments, such as measuring spasticity, were conducted using the Modified Modified Ashworth Scale (MMAS). Functional outcomes were also evaluated, using the Timed Up and Go (TUG) test, as well as the Timed 10-Meter Walk Test (WTT). Friedman test in SPSS version 22.0 was used to analysis the response variables with respect to each stage of evaluation. Spearman rank correlation was also used to measure correlation among clinical assessments and functional outcomes.

Results: The comparison between two groups showed significant differences only in the Modified Modified Ashworth Scale and Visual Analogue Scale (VAS) post treatment. The experimental group showed significant differences in the MMAS (p = 0.002), WTT (p < 0.001), and TUG (p < 0.001) scores. Nevertheless, the scores of the control group were not significantly different in different stages of evaluation.

Conclusion: The findings of the study suggest that using functional stretching exercises can improve functional outcomes in chronic spastic stroke patients.
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October 2018

The Effect of Functional Stretching Exercises on Neural and Mechanical Properties of the Spastic Medial Gastrocnemius Muscle in Patients with Chronic Stroke: A Randomized Controlled Trial.

J Stroke Cerebrovasc Dis 2018 Jul 26;27(7):1733-1742. Epub 2018 Apr 26.

Department of Neurology & Isfahan Neurosciences Research Center, Faculty of Medical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Following spasticity, neural and mechanical changes of the paretic muscle often occur, which affect the muscle function. The aim of this study was to investigate the effect of functional stretching exercises on neural and mechanical properties of the spastic muscle in patients with stroke.

Materials And Methods: This study was a single-blinded, randomized control trial. Forty five patients with stroke (experimental group: n = 30; control group: n = 15) participated in this study. Subjects in the experimental group participated in a functional stretching program 3 times a week for 4 weeks. Subjects in both groups were evaluated before the training, at the end of training, and then during a 2-month follow-up. Neural properties, including H-reflex latency and Hmax/Mmax ratio, were acquired. Mechanical properties, including fascicle length, pennation angle, and muscle thickness in the spastic medial gastrocnemius muscle, were evaluated. Repeated measure analysis of variance was used in the analysis.

Results: Time by group interaction in the pennation angle (P = .006), and in muscle thickness (P = .030) was significant. The results indicated that the H-reflex latency (P = .006), pennation angle (P < .001), and muscle thickness (P = .001) were altered after stretching training program and these changes were at significant level after 2-month follow-up.

Conclusion: The results indicated that the use of functional stretching exercises can cause significant differences in neural and mechanical properties of spastic medial gastrocnemius muscle in patients with chronic stroke.
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July 2018

Effect of type of luting agents on stress distribution in the bone surrounding implants supporting a three-unit fixed dental prosthesis: 3D finite element analysis.

Dent Res J (Isfahan) 2015 Jan-Feb;12(1):57-63

School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Osseointegration of dental implants is influenced by many biomechanical factors that may be related to stress distribution. The aim of this study was to evaluate the effect of type of luting agent on stress distribution in the bone surrounding implants, which support a three-unit fixed dental prosthesis (FDP) using finite element (FE) analysis.

Materials And Methods: A 3D FE model of a three-unit FDP was designed replacing the maxillary first molar with maxillary second premolar and second molar as the abutments using CATIA V5R18 software and analyzed with ABAQUS/CAE 6.6 version. The model was consisted of 465108 nodes and 86296 elements and the luting agent thickness was considered 25 μm. Three load conditions were applied on eight points in each functional cusp in horizontal (57.0 N), vertical (200.0 N) and oblique (400.0 N, θ = 120°) directions. Five different luting agents were evaluated. All materials were assumed to be linear elastic, homogeneous, time independent and isotropic.

Results: For all luting agent types, the stress distribution pattern in the cortical bone, connectors, implant and abutment regions was almost uniform among the three loads. Furthermore, the maximum von Mises stress of the cortical bone was at the palatal side of second premolar. Likewise, the maximum von Mises stress in the connector region was in the top and bottom of this part.

Conclusion: Luting agents transfer the load to cortical bone and different types of luting agents do not affect the pattern of load transfer.
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February 2015

Evaluation of resistance form of different preparation features on mandibular molars.

Indian J Dent Res 2013 Mar-Apr;24(2):216-9

Department of Fixed Prosthodontics, Faculty of Dentistry, Esfahan University of Medical Sciences, Iran.

Aims: Resistance form of full metal-ceramic crown is an important feature that determines longevity of these restorations. This study evaluated the resistance form of full metal-ceramic prepared with four different design features.

Materials And Methods: An acrylic tooth was prepared with 20° total occlusal convergence (TOC) angle, 2.5 mm of occlusocervical dimension and a shoulder finishing line. This design lacked resistance form. The crown preparation was subsequently modified by preparing Mesial Occlusal Distal isthmus, placing occlusal inclined plane, and reducing TOC. Four metal dies from these designs were constructed by lathe machine and then 10 metal copings were fabricated for each preparation. Metal coping were cemented on metal dies with temp-bond cement. Force was applied at 45° from lingual to buccal direction with universal testing machine.

Statistical Analysis Used: The data were evaluated by Kruskal-Wallis and non-parametric Mann-Whitney test.

Results: All features increased resistance form when compare to control group. However, reduce TOC group showed greatest value of resistance.

Conclusion: Within the limitation of this study, reducing the tapering of occlusocervival dimension is the most effective way in increasing resistance form, although, other features were also effective.
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September 2015

The effect of three different disinfection materials on alginate impression by spray method.

ISRN Dent 2012 25;2012:695151. Epub 2012 Jul 25.

Dental Implant Research Center, School of Dentistry, Isfahan University of Medical sciences, 81746-73461 Isfahan, Iran.

Introduction. The aim of this study was to investigate the effect of three different types of disinfectant agents on alginate impression material after 5 and 10 minutes. Method and Materials. In this in vitro experimental study, 66 circular samples of alginate impression material were contaminated with Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans fungus. Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex, and Epimax by way of spraying. Afterwards, they were kept in plastic bags with humid rolled cotton for 5 and 10 minutes. The number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. Statistical Mann-Whitney test was used for data analysis (α = 0.05). Results. After 5 minutes, Epimax showed the highest disinfection action on Staphylococcus aureus as it completely eradicated the bacteria. The disinfection capacity of different agents can be increased as time elapses except for Pseudomonas aeruginosa which was eradicated completely in both 5 and 10 minutes. Conclusion. This study revealed that alginate can be effectively disinfected by three types of disinfecting agents by spraying method, although Epimax showed the highest disinfection action after 10 minutes compared to other agents.
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August 2012

Implant-retained mandibular bar-supported overlay dentures: a finite element stress analysis of four different bar heights.

J Oral Implantol 2012 Apr 14;38(2):133-9. Epub 2010 Jun 14.

Department of Fixed Prosthodontics, Torabinejad Research Center, School of Dental Medicine, Isfahan University of Medical Sciences (MUI), Isfahan, Iran.

Proper stress distribution on dental implants is necessary in bar-retained implant overlay dentures. We aimed to comparatively assess this stress distribution according to different bar heights using finite element models. A three-dimensional (3D) computer model of mandible with 2 implants (ITI, 4.1 mm diameter and 12 mm length) in canine areas and an overlying implant-supported bar-retained overlay denture were simulated with 0-, 1-, 2-, and 3-mm bar heights using ABAQUS software. A vertical force was applied to the left first molar and gradually increased from 0 to 50 N. The resultant stress distribution was evaluated. Bars of 1 and 2 mm in height transferred the least stress to the implants (3.882 and 3.896 MPa, respectively). The 0-mm height of the bar connection transferred the highest stress value (4.277 MPa). The amount of stress transferred by 3-mm heights of the bar connection was greater than that of 1- and 2-mm bar connections and smaller than that of 0-mm bar connection (4.165 kgN). This 3D finite element analysis study suggested that the use of Dolder bar attachment with 1- and 2-mm heights could be associated with appropriate stress distribution for implant-retained overlay dentures.
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April 2012

Survey of both hepatitis B virus (HBsAg) and hepatitis C virus (HCV-Ab) coinfection among HIV positive patients.

Virol J 2009 Nov 18;6:202. Epub 2009 Nov 18.

Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.

Background: HIV, HBV and HCV is major public health concerns. Because of shared routes of transmission, HIV-HCV coinfection and HIV-HBV coinfection are common. HIV-positive individuals are at risk of coinfection with HBV and HCV infections. The prevalence rates of coinfection with HBV and HCV in HIV-patients have been variable worldwide depending on the geographic regions, and the type of exposure.

Aim: This study aimed to examine HBV and HCV coinfection serologically and determine the shared and significant factors in the coinfection of HIV-positive patients.

Methods: This descriptive, cross-sectional study was carried out on 391 HIV-positive patients including 358 males and 33 females in Lorestan province, west Iran, to survey coinfection with HBsAg and anti-HCV. The retrospective demographic data of the subjects was collected and the patients' serums were analyzed by ELISA kits including HBsAg and anti-HCV. The collected data was analyzed with SPSS software (15) and Chi-square. Fisher's exact test with 5% error intervals was used to measure the correlation of variables and infection rates.

Results: The results of the study indicated that the prevalence of coinfection in HIV-positive patients with hepatitis viruses was 94.4% (370 in 391), out of whom 57 (14.5%) cases were HBsAg positive, 282 (72%) cases were anti-HCV positive, and 31 (7.9%) cases were both HBsAg and anti-HCV positive.

Conclusion: There was a significant correlation between coinfection with HCV and HBV and/or both among HIV-positive patients depending on different variables including sex, age, occupation, marital status, exposure to risk factors. (p < 0.001).
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November 2009