Publications by authors named "Edyta M Augustyniak"

2 Publications

  • Page 1 of 1

Electrically tunable fluidic lens imaging system for laparoscopic fluorescence-guided surgery.

Biomed Opt Express 2017 Jul 13;8(7):3232-3247. Epub 2017 Jun 13.

CR-UK/MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, OX3 7DQ, UK.

The addition of fluorescence guidance in laparoscopic procedures has gained significant interest in recent years, particularly through the use of near infrared (NIR) markers. In this work we present a novel laparoscope camera coupler based on an electrically tunable fluidic lens that permits programmable focus control and has desirable achromatic performance from the visible to the NIR. Its use extends the lower working distance limit and improves detection sensitivity, important for work with molecularly targeted fluorescence markers. We demonstrate its superior optical performance in laparoscopic fluorescence-guided surgery. results using a tumor specific molecular probe and a nonspecific NIR dye are presented.
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http://dx.doi.org/10.1364/BOE.8.003232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508825PMC
July 2017

CD4(+) T cell surface alpha enolase is lower in older adults.

Mech Ageing Dev 2015 Dec 1;152:56-62. Epub 2015 Oct 1.

Life and Health Sciences, Aston Research Centre for Healthy Ageing, Aston University, Birmingham B4 7ET, UK. Electronic address:

To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4(+) T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25 years) and older (n=10; 50-70 years) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4(+) T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4(+) T cells (p<0.05). In an independent age-matched case-control study, lower CD4(+) T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.
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http://dx.doi.org/10.1016/j.mad.2015.09.005DOI Listing
December 2015