Edward L Lecluyse

Edward L Lecluyse

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Edward L Lecluyse

Edward L Lecluyse

Publications by authors named "Edward L Lecluyse"

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Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening.

Toxicol Sci 2020 Mar;174(1):63-78

National Center for Computational Toxicology, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina.

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http://dx.doi.org/10.1093/toxsci/kfz238DOI Listing
March 2020

Long-Term Engineered Cultures of Primary Mouse Hepatocytes for Strain and Species Comparison Studies During Drug Development.

Gene Expr 2019 11 24;19(3):199-214. Epub 2019 Jul 24.

School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USA.

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http://dx.doi.org/10.3727/105221619X15638857793317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827040PMC
November 2019

Advancing nonclinical innovation and safety in pharmaceutical testing.

Drug Discov Today 2019 02 20;24(2):624-628. Epub 2018 Nov 20.

Physicians Committee for Responsible Medicine, 5100 Wisconsin Ave NW, Ste 400, Washington, DC 20016, USA.

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http://dx.doi.org/10.1016/j.drudis.2018.11.011DOI Listing
February 2019

Dose-response in a high density three-dimensional liver device with real-time bioenergetic and metabolic flux quantification.

Toxicol In Vitro 2017 Dec 1;45(Pt 1):119-127. Epub 2017 Sep 1.

Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States. Electronic address:

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http://dx.doi.org/10.1016/j.tiv.2017.08.021DOI Listing
December 2017

Editor's Highlight: Modeling Compound-Induced Fibrogenesis In Vitro Using Three-Dimensional Bioprinted Human Liver Tissues.

Toxicol Sci 2016 12 7;154(2):354-367. Epub 2016 Sep 7.

Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.

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http://dx.doi.org/10.1093/toxsci/kfw169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5139067PMC
December 2016

The Promise of New Technologies to Reduce, Refine, or Replace Animal Use while Reducing Risks of Drug Induced Liver Injury in Pharmaceutical Development.

ILAR J 2016 12;57(2):186-211

Frank D. Sistare, PhD, is Associate Vice President, Safety Assessment and Laboratory Animal Resources at Merck and Co., Inc. in West Point, PA. William B. Mattes, PhD, DABT, is Director, Division of Systems Biology at the Food and Drug Administration's National Center for Toxicological Research in Jefferson, AR. Edward L. LeCluyse, PhD, is Principal Scientist at LifeNet Health in Research Triangle Park, NC.

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http://dx.doi.org/10.1093/ilar/ilw025DOI Listing
December 2016

Subtoxic Alterations in Hepatocyte-Derived Exosomes: An Early Step in Drug-Induced Liver Injury?

Toxicol Sci 2016 06 8;151(2):365-75. Epub 2016 Mar 8.

Division of Pharmacotherapy and Experimental Therapeutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599; Institute for Drug Safety Sciences, University of North Carolina at Chapel Hill, Research Triangle Park, North Carolina 27709;

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http://dx.doi.org/10.1093/toxsci/kfw047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880137PMC
June 2016

Co-culture of Hepatocytes and Kupffer Cells as an In Vitro Model of Inflammation and Drug-Induced Hepatotoxicity.

J Pharm Sci 2016 Feb;105(2):950-964

The Institute for Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709; The Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514. Electronic address:

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http://dx.doi.org/10.1016/S0022-3549(15)00192-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330391PMC
February 2016

A micropatterned hepatocyte coculture model for assessment of liver toxicity using high-content imaging analysis.

Assay Drug Dev Technol 2014 Jan-Feb;12(1):16-27. Epub 2014 Jan 20.

1 The Hamner Institutes for Health Sciences , Research Triangle Park, North Carolina.

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http://dx.doi.org/10.1089/adt.2013.525DOI Listing
October 2014

Development of 3D dynamic flow model of human liver and its application to prediction of metabolic clearance of 7-ethoxycoumarin.

Tissue Eng Part C Methods 2014 Aug 4;20(8):641-51. Epub 2014 Mar 4.

1 The Hamner Institutes for Health Sciences, Research Triangle Park , North Carolina.

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http://dx.doi.org/10.1089/ten.TEC.2013.0562DOI Listing
August 2014

Liver biomarker and in vitro assessment confirm the hepatic origin of aminotransferase elevations lacking histopathological correlate in beagle dogs treated with GABAA receptor antagonist NP260.

Toxicol Appl Pharmacol 2014 Jun 31;277(2):131-7. Epub 2014 Mar 31.

The Institute for Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC 27709, USA.

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http://dx.doi.org/10.1016/j.taap.2014.03.015DOI Listing
June 2014

A map of the PPARα transcription regulatory network for primary human hepatocytes.

Chem Biol Interact 2014 Feb 22;209:14-24. Epub 2013 Nov 22.

The Hamner Institutes for Health Sciences, Institute for Chemical Safety Sciences, 6 Davis Drive, PO Box 12137, Research Triangle Park, NC 27709, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cbi.2013.11.006DOI Listing
February 2014

Knockout of the aryl hydrocarbon receptor results in distinct hepatic and renal phenotypes in rats and mice.

Toxicol Appl Pharmacol 2013 Oct 13;272(2):503-18. Epub 2013 Jul 13.

The Hamner Institute for Health Sciences, Institute for Chemical Safety Sciences, RTP, NC 27709, USA.

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https://linkinghub.elsevier.com/retrieve/pii/S0041008X130029
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http://dx.doi.org/10.1016/j.taap.2013.06.024DOI Listing
October 2013

Bioactivation and toxicity of acetaminophen in a rat hepatocyte micropatterned coculture system.

J Biochem Mol Toxicol 2013 Oct 5;27(10):471-8. Epub 2013 Aug 5.

Hepregen Corporation, Medford, MA, 02155, U.S.A.

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http://dx.doi.org/10.1002/jbt.21512DOI Listing
October 2013

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.

Arch Toxicol 2013 Aug 23;87(8):1315-530. Epub 2013 Aug 23.

Leibniz Research Centre for Working Environment and Human Factors (IFADO), 44139, Dortmund, Germany.

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http://dx.doi.org/10.1007/s00204-013-1078-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3753504PMC
August 2013

models for liver toxicity testing.

Toxicol Res (Camb) 2013 Jan 23;2(1):23-39. Epub 2012 Nov 23.

Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599, USA.

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http://xlink.rsc.org/?DOI=C2TX20051A
Publisher Site
http://dx.doi.org/10.1039/C2TX20051ADOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593300PMC
January 2013

Organotypic liver culture models: meeting current challenges in toxicity testing.

Crit Rev Toxicol 2012 Jul 15;42(6):501-48. Epub 2012 May 15.

The Institute for Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA.

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http://dx.doi.org/10.3109/10408444.2012.682115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423873PMC
July 2012

Cross-species comparisons of transcriptomic alterations in human and rat primary hepatocytes exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Toxicol Sci 2012 May 1;127(1):199-215. Epub 2012 Feb 1.

The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709, USA.

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http://dx.doi.org/10.1093/toxsci/kfs069DOI Listing
May 2012

Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity.

Toxicol Appl Pharmacol 2010 Dec 24;249(3):208-16. Epub 2010 Sep 24.

Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, NC 27599, USA.

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http://dx.doi.org/10.1016/j.taap.2010.09.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2980592PMC
December 2010

Incorporating human dosimetry and exposure into high-throughput in vitro toxicity screening.

Toxicol Sci 2010 Oct 16;117(2):348-58. Epub 2010 Jul 16.

National Center for Computational Toxicology, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA.

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http://dx.doi.org/10.1093/toxsci/kfq220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280841PMC
October 2010

Isolation and culture of primary hepatocytes from resected human liver tissue.

Methods Mol Biol 2010 ;640:57-82

CellzDirect | Invitrogen Corp., Durham, NC, USA.

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http://dx.doi.org/10.1007/978-1-60761-688-7_3DOI Listing
October 2010

Xenobiotic-metabolizing enzyme and transporter gene expression in primary cultures of human hepatocytes modulated by ToxCast chemicals.

J Toxicol Environ Health B Crit Rev 2010 Feb;13(2-4):329-46

US Environmental Protection Agency, Office of Research and Development, National Center for Computational Toxicology, Research Triangle Park, North Carolina, USA.

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http://dx.doi.org/10.1080/10937404.2010.483949DOI Listing
February 2010

Regulation of cytochrome P450 2C9 expression in primary cultures of human hepatocytes.

J Biochem Mol Toxicol 2009 Jan-Feb;23(1):43-58

Department of Pharmacokinetics Pharmacodynamics and Metabolism, Pfizer Global Research and Development, Ann Arbor, MI 48105, USA.

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http://dx.doi.org/10.1002/jbt.20264DOI Listing
July 2009

Primary human hepatocyte culture for HCV study.

Methods Mol Biol 2009 ;510:373-82

Department of Pathology Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, USA.

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http://dx.doi.org/10.1007/978-1-59745-394-3_28DOI Listing
March 2009

Antiplatelet agents aspirin and clopidogrel are hydrolyzed by distinct carboxylesterases, and clopidogrel is transesterificated in the presence of ethyl alcohol.

J Pharmacol Exp Ther 2006 Dec 30;319(3):1467-76. Epub 2006 Aug 30.

Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA.

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http://dx.doi.org/10.1124/jpet.106.110577DOI Listing
December 2006

Anti-influenza prodrug oseltamivir is activated by carboxylesterase human carboxylesterase 1, and the activation is inhibited by antiplatelet agent clopidogrel.

J Pharmacol Exp Ther 2006 Dec 11;319(3):1477-84. Epub 2006 Sep 11.

Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA.

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http://dx.doi.org/10.1124/jpet.106.111807DOI Listing
December 2006

Cloning, tissue expression, and regulation of beagle dog CYP4A genes.

Toxicol Sci 2006 Aug 4;92(2):356-67. Epub 2006 May 4.

Division of Molecular Pharmaceutics, School of Pharmacy, The University of North Carolina at Chapel Hill, 27599, USA.

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http://dx.doi.org/10.1093/toxsci/kfl009DOI Listing
August 2006

Differential regulation of hepatic CYP2B6 and CYP3A4 genes by constitutive androstane receptor but not pregnane X receptor.

J Pharmacol Exp Ther 2006 Jun 2;317(3):1200-9. Epub 2006 Mar 2.

Division of Molecular Pharmaceutics, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7360, USA.

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http://jpet.aspetjournals.org/cgi/doi/10.1124/jpet.105.09816
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http://dx.doi.org/10.1124/jpet.105.098160DOI Listing
June 2006

Temporal kinetics and concentration-response relationships for induction of CYP1A, CYP2B, and CYP3A in primary cultures of beagle dog hepatocytes.

J Biochem Mol Toxicol 2006 ;20(2):69-78

Division of Molecular Pharmaceutics, School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

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http://dx.doi.org/10.1002/jbt.20118DOI Listing
June 2006

Differential UGT1A1 induction by chrysin in primary human hepatocytes and HepG2 Cells.

J Pharmacol Exp Ther 2005 Dec 31;315(3):1256-64. Epub 2005 Aug 31.

Division of Drug Delivery and Disposition, School of Pharmacy, CB 7360, Kerr Hall, Room 2319, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7360, USA.

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http://dx.doi.org/10.1124/jpet.105.090795DOI Listing
December 2005

Human CYP2C8 is transcriptionally regulated by the nuclear receptors constitutive androstane receptor, pregnane X receptor, glucocorticoid receptor, and hepatic nuclear factor 4alpha.

Mol Pharmacol 2005 Sep 2;68(3):747-57. Epub 2005 Jun 2.

Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.

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http://dx.doi.org/10.1124/mol.105.013169DOI Listing
September 2005

Modulation of UDP-glucuronosyltransferase 1A1 in primary human hepatocytes by prototypical inducers.

J Biochem Mol Toxicol 2005 ;19(2):96-108

Division of Drug Delivery and Disposition, School of Pharmacy, CB7360, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

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http://dx.doi.org/10.1002/jbt.20058DOI Listing
August 2005

Isolation and culture of primary human hepatocytes.

Methods Mol Biol 2005 ;290:207-29

Department of Drug Delivery and Disposition, University of North Carolina, School of Pharmacy, Chapel Hill, USA.

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http://dx.doi.org/10.1385/1-59259-838-2:207DOI Listing
March 2005

Effects of avasimibe on cytochrome P450 2C9 expression in vitro and in vivo.

Drug Metab Dispos 2004 Dec 27;32(12):1370-6. Epub 2004 Aug 27.

Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA.

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http://dx.doi.org/10.1124/dmd.104.000208DOI Listing
December 2004

The human sulfotransferase SULT1A1 gene is regulated in a synergistic manner by Sp1 and GA binding protein.

Mol Pharmacol 2004 Dec 21;66(6):1690-701. Epub 2004 Sep 21.

Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia.

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http://dx.doi.org/10.1124/mol.104.003350DOI Listing
December 2004

Interindividual variation in the metabolism of arsenic in cultured primary human hepatocytes.

Toxicol Appl Pharmacol 2004 Dec;201(2):166-77

Department of Pediatrics, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

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http://dx.doi.org/10.1016/j.taap.2004.05.004DOI Listing
December 2004

P-glycoprotein expression, localization, and function in sandwich-cultured primary rat and human hepatocytes: relevance to the hepatobiliary disposition of a model opioid peptide.

Pharm Res 2004 Jul;21(7):1294-302

Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

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http://dx.doi.org/10.1023/b:pham.0000033018.97745.0dDOI Listing
July 2004

Role of orphan nuclear receptors in the regulation of drug-metabolising enzymes.

Clin Pharmacokinet 2003 ;42(15):1331-57

Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina at Chapel Hill, North Carolina 27599, USA.

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http://dx.doi.org/10.2165/00003088-200342150-00003DOI Listing
May 2004

Regulation of CYP2B6 in primary human hepatocytes by prototypical inducers.

Drug Metab Dispos 2004 Mar;32(3):348-58

Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7360, USA.

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http://dx.doi.org/10.1124/dmd.32.3.348DOI Listing
March 2004

Kupffer cell-mediated IL-2 suppression of CYP3A activity in human hepatocytes.

Drug Metab Dispos 2004 Mar;32(3):359-63

School of Pharmacy, Division of Pharmacotherapy, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599-7360, USA.

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http://dx.doi.org/10.1124/dmd.32.3.359DOI Listing
March 2004

Avasimibe induces CYP3A4 and multiple drug resistance protein 1 gene expression through activation of the pregnane X receptor.

J Pharmacol Exp Ther 2003 Sep 23;306(3):1027-34. Epub 2003 May 23.

Department of Pharmacokinetics, Pfizer Global Research and Development, Ann Arbor MI 48105, USA.

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http://dx.doi.org/10.1124/jpet.103.050526DOI Listing
September 2003

Glucocorticoid receptor enhancement of pregnane X receptor-mediated CYP2B6 regulation in primary human hepatocytes.

Drug Metab Dispos 2003 May;31(5):620-30

Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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http://dx.doi.org/10.1124/dmd.31.5.620DOI Listing
May 2003

A novel distal enhancer module regulated by pregnane X receptor/constitutive androstane receptor is essential for the maximal induction of CYP2B6 gene expression.

J Biol Chem 2003 Apr 5;278(16):14146-52. Epub 2003 Feb 5.

Division of Drug Delivery and Disposition, School of Pharmacy, University of North Carolina, Chapel Hill 27599, USA.

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http://dx.doi.org/10.1074/jbc.M212482200DOI Listing
April 2003

Comparative effects of thiazolidinediones on in vitro P450 enzyme induction and inhibition.

Drug Metab Dispos 2003 Apr;31(4):439-46

Department of Pharmacokinetics Pharmacodynamics and Metabolism, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA.

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http://dx.doi.org/10.1124/dmd.31.4.439DOI Listing
April 2003

Selenium compounds modulate the activity of recombinant rat AsIII-methyltransferase and the methylation of arsenite by rat and human hepatocytes.

Chem Res Toxicol 2003 Mar;16(3):261-5

Department of Pediatrics, Curriculum in Toxicology, Division of Drug Delivery and Disposition, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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http://dx.doi.org/10.1021/tx025649rDOI Listing
March 2003

Regulation of human CYP2C9 by the constitutive androstane receptor: discovery of a new distal binding site.

Mol Pharmacol 2002 Sep;62(3):737-46

Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.

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http://dx.doi.org/10.1124/mol.62.3.737DOI Listing
September 2002

The effect of cyclophosphamide with and without dexamethasone on cytochrome P450 3A4 and 2B6 in human hepatocytes.

Drug Metab Dispos 2002 Jul;30(7):814-22

Division of Pharmacotherapy, School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.

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http://dx.doi.org/10.1124/dmd.30.7.814DOI Listing
July 2002