We are pioneer in the study of fibrinolytic microvesicles (MVs) as functional messengers of plasmin generation. MVs derived from cells that express plasminogen activators may generate, disseminate and transfer plasmin proteolytic activity (Blood, 2007). For instance, besides endothelial cells and leucocytes, neurons are able to release microvesicles bearing plasmin and tPA activity (Biochem J 2010). We have found that endothelial cell- and leucocyte- derived microvesicles bear the machinery necessary for plasmin formation including plasminogen activators and its receptors: tPA for endothelial MVs and the uPA/uPAR system for leukocyte MVs (Haematologica 2013). Presently, we are prospecting on synthetic tools to identify phosphatidylserine on cell membranes.
Furthermore, we have discovered a new mechanism for plasmin formation that bypass the requirement for co-assembly of plasminogen and uPA on the same surface (Blood 2010). These heterotypic cell-to-cell (platelets/ monocytes; platelets/MPs), cell-to-matrix (leukocytes/fibrin), or MPs-to-matrix (MPs/fibrin) proteolytic cross-talk represents an alternative pathway for localized plasmin formation that may be relevant not only for fibrinolysis, but also for processes such as cell migration and MP dissemination. Finally, our data provide additional evidence for a novel role of MPs and platelets, as vectors that generate and propagate plasmin fibrinolytic and/or proteolytic activity, and could thereby constitute a pharmacologic tool.
At the Inserm reaserch unit UMR_S 1140 (Faculty of pharmaceutical and biological sciences, Paris) we have studied the fibrinolytic and microvesiculation properties of human bone marrow mesenchymal stem cells (submitted). Our translational research is mainly related to the role of NET transformation of PMN on the fibrinolytic response to ischaemic diseases.

Primary Affiliation: Inserm/Université Paris Descartes - Paris , France


Sizing nanomatter in biological fluids by fluorescence single particle tracking.
Nano Lett 2010 Nov 5;10(11):4435-42. Epub 2010 Oct 5.
Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000 Gent, Belgium.

Plasmin induces apoptosis of aortic valvular myofibroblasts.
J Pathol 2010 May;221(1):37-48
Inserm, UMR698, Hemostasis, Bio-Engineering and Cardiovascular Remodelling, Paris 7 Denis Diderot University, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75877 Paris Cedex 18, France.

Activation of plasminogen into plasmin at the surface of endothelial microparticles: a mechanism that modulates angiogenic properties of endothelial progenitor cells in vitro.
Blood 2007 Oct 2;110(7):2432-9. Epub 2007 Jul 2.
Unité Mixte de Recherche S 608 (UMR-S 608), Institut National de la Santé et de la Recherche Médicale (INSERM), Université de la Méditerranée, Unité de Formation et de Recherche (UFR) de Pharmacie, Marseille, France.

Interaction of fibrin(ogen) with apolipoprotein(a): further characterization and identification of a novel lysine-dependent apolipoprotein(a)-binding site within the gamma chain 287-411 region.
Biochemistry 2006 Sep;45(35):10624-32
Center for Vascular and Inflammatory Diseases and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.

Functional characterization of fibrinogen Bicêtre II: a gamma 308 Asn-->Lys mutation located near the fibrin D:D interaction sites.
Blood Coagul Fibrinolysis 2006 Apr;17(3):193-201
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

The plasminogen-MMP system is more activated in the scar than in viable myocardium 3 months post-MI in the rat.
J Mol Cell Cardiol 2005 Jan 9;38(1):193-204. Epub 2004 Dec 9.
Inserm U460, Cardiovascular Remodeling, CHU Xavier Bichat-Claude Bernard, 46, rue Henri Huchard, 75877 Paris cedex 18, France.

Involvement of the mural thrombus as a site of protease release and activation in human aortic aneurysms.
Am J Pathol 2002 Nov;161(5):1701-10
Institut National de la Santé et de la Recherche Médicale U460, Cardiovascular Remodeling, Hôpital Xavier Bichat, Paris, France.

Bivalency of plasminogen monoclonal antibodies is required for plasminogen bridging to fibrin and enhanced plasmin formation.
Biochim Biophys Acta 2002 Jul;1598(1-2):165-76
Plasminogen Activation in Cardiovascular Remodelling, Institut National de la Santé et de la Recherche Médicale, INSERM U460, UFR de Médecine Xavier Bichat, 16 rue Henri Huchard-BP 416, Paris, France.

Evidence of Netosis in Septic Shock-Induced Disseminated Intravascular Coagulation.
Shock 2017 03;47(3):313-317
*Medical Intensive Care Unit, NHC - Strasbourg University Hospital, Strasbourg, France †EA 7293 FMTS, University of Strasbourg, Strasbourg, France ‡Laboratory of Medical Biostatistics and Informatics, University of Strasbourg, Strasbourg, France §Group Method in Clinical Research, Public Health Unit, Strasbourg University Hospital, Strasbourg, France ||Hematology and heamostasis laboratory, Hautepierre - Strasbourg University Hospital, Strasbourg, France ¶EA 3430 FMTS, University of Strasbourg, Strasbourg, France #EA 7290, University of Strasbourg, Strasbourg, France **CNRS UMR 7213, University of Strasbourg, Illkirch, France ††Paris Descartes University, Sorbonne, Paris, France ‡‡INSERM UMR S1140, Paris, France.

Lipoprotein(a) and homocysteine potentiate the risk of coronary artery disease in male subjects.
Circ J 2012 18;76(8):1953-7. Epub 2012 May 18.
Instituto Nacional de Cardiología Ignacio Chávez, Grupo Genética Intervencionista, Departamentos de Biología Molecular, Hemodinámica, Endocrinología, Unidad Coronaria, Juan Badiano 1, México.