Publications by authors named "Ederlan de Souza Ferreira"

11 Publications

  • Page 1 of 1

Long-term cryopreservation of Lentinus crinitus strains by wheat grain technique.

J Microbiol Methods 2022 May 16:106491. Epub 2022 May 16.

Universidade Federal da Bahia, Salvador, Bahia, Brazil. Electronic address:

Lentinus crinitus (Basidiomycota: Polyporales) is a saprophytic fungus with biotechnological importance described more than 20 years ago. However, there are few studies on the long-term preservation of this basidiomycete. Cryopreservation is a long-term storage technique that reduces the metabolic activity of microorganisms, but its success depends on the adjustment of the freezing process, the cryoprotectants, and the protective substrates for each species. This study aimed to assess the mycelial viability and genetic stability of L. crinitus strains cryopreserved at -86 °C for two years by the wheat grain technique using different cryoprotectants and freezing methods. Three strains of L. crinitus (U9-1, U13-5, and U15-12) were subjected to different concentrations and types of cryoprotectants (dimethyl sulfoxide, glycerol, glucose, and sucrose), freezing methods such as immediate freezing from 25 to -86 °C and progressing freezing from 25 to -86 °C in a freezing container with isopropyl alcohol to control the rate of cell freezing at -1 °C min, protective substrate (wheat grain and 2% malt extract agar), and cryopreservation period (1, 6, 12, and 24 months). After thawing, samples were evaluated for mycelial viability, time to mycelial recovery, mycelial stability, and genetic stability of the fungus. All techniques achieved effective cryopreservation at -86 °C, mainly with the wheat grain technique. All cryoprotectants (3.5% glycerol, 1.5% dimethyl sulfoxide, 25% sucrose, and 5% glucose), freezing methods (immediate and gradual), and protective substrate (wheat grain and malt extract agar) were effective for cryopreservation of the three L. crinitus strains in an ultra-low temperature freezer for two years. Mycelial viability, mycelial stability, and genetic stability of the fungus were not affected after two-year cryopreservation, evidencing the robustness of the long-term cryopreservation technique and the fungus.
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http://dx.doi.org/10.1016/j.mimet.2022.106491DOI Listing
May 2022

Basidiocarp structures of Lentinus crinitus: an antimicrobial source against foodborne pathogens and food spoilage microorganisms.

World J Microbiol Biotechnol 2022 Mar 17;38(5):74. Epub 2022 Mar 17.

Universidade Federal da Bahia, Rua Augusto Viana, s/n, 40110-909, Salvador, BA, Brazil.

Lentinus crinitus basidiocarps are an alternative to antimicrobials, but the stipe (24% basidiocarp) is discarded even with potential antimicrobial activity. This study evaluated the antimicrobial activity of L. crinitus basidiocarp pileus and stipe extracts against foodborne pathogens and food spoilage microorganisms. Basidiocarps of L. crinitus were grown in sugarcane bagasse and rice husks and the pileus and stipe methanolic extract was analyzed by broth microdilution method for antimicrobial activity against eight bacteria and eight fungi. The minimum bactericidal concentration values for pileus and stipe ranged from 0.40 to 0.50 mg mL, for streptomycin from 0.10 to 0.50 mg mL, and for ampicillin from 0.40 to 1.20 mg mL. The minimum fungicidal concentration values for pileus and stipe ranged from 0.06 to 0.60 mg mL, for bifonazole from 0.20 to 0.25 mg mL, and for ketoconazole from 0.30 to 3.50 mg mL. Extracts had bacteriostatic, bactericidal, fungistatic and fungicidal activity against all microorganisms, but with greater efficiency and specificity for some microorganisms. Both pileus and stipe are promising and sustainable alternatives for use in food, agricultural, and pharmaceutical industries.
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http://dx.doi.org/10.1007/s11274-022-03257-wDOI Listing
March 2022

Syrah Grape Skin Residues Has Potential as Source of Antioxidant and Anti-Microbial Bioactive Compounds.

Biology (Basel) 2021 Dec 3;10(12). Epub 2021 Dec 3.

Department of Biotechnology, Health Science Institute, Federal University of Bahia (UFBA), Salvador 40170-115, BA, Brazil.

In this study, we evaluated the effects of ultrasound-assisted extraction (UAE) under different time-temperature conditions on the content of bioactive compounds, antioxidant and antimicrobial activities of Syrah grape skin residue. The application of UAE showed a positive effect on the extraction of total flavonoids, and a negative effect on total polyphenols. The temperature of 40 °C and 60 °C without the UAE caused an increase of 260% and 287% of the total polyphenols, respectively. Nineteen individually bioactive compounds were quantified. The anthocyanin concentration (malvidin-3,5-di-O-glucoside 118.8-324.5 mg/100 g) showed high variation, to a lesser extent for phenolic acids, flavonoids, flavonols, procyanidins and stilbenes due to the UAE process. The Syrah grape skin residue has a high concentration of total phenolic compounds of 196-733.7 mg·GAE/100 g and a total flavonoid content of 9.8-40.0 mg·QE/100 g. The results of free radical scavenging activity (16.0-48.7 mg/100 mL, as EC) and its inhibition of microbial growth (0.16 mg/mL, as EC for , and 0.04 mg/mL, as EC for ) by grape skin extract (UAE 40:20) indicate high antioxidant and antibacterial activity. It was concluded that the use of ultrasound needs further analysis for its application in this context, as it has shown deleterious effects on some compounds of interest. Syrah grape skin residue has potential as a source of bioactive antioxidants, antimicrobial activity and for use as a functional food ingredient.
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http://dx.doi.org/10.3390/biology10121262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8698917PMC
December 2021

Antimicrobial activity of leaf oleoresin.

Nat Prod Res 2021 Dec 5:1-7. Epub 2021 Dec 5.

Graduate Program in Biotechnology Applied to Agriculture, Paranaense University, Umuarama, Brazil.

Bioactive compounds extracted from plants such as antimicrobials have attracted the attention of consumers and the food industry. This study aimed to determine the antimicrobial activity and chemical composition of leaf oleoresin obtained by supercritical CO extraction. The oleoresin was obtained by supercritical CO extraction and the chemical identification by gas chromatography coupled to mass spectrometry. Antimicrobial activity was evaluated by broth microdilution method against 14 foodborne fungi and bacteria. The oleoresin major chemical class was phytosterols (22.7%) and the major compounds were γ-sitosterol (15.7%), α-tocopherol (13.7%), phytol (13.1%), and hexadecanoic acid (11.5%). Minimum inhibitory concentration against bacteria ranged from 0.0025 to 0.010 mg mL. The oleoresin had high bactericidal activity against all bacteria, mainly and with 0.005 mg mL minimum bactericidal concentration. However, it had low fungicidal activity. The leaf oleoresin of has promising applications in food, cosmetic, and pharmaceutical industries.
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http://dx.doi.org/10.1080/14786419.2021.2011270DOI Listing
December 2021

Effect of Drying Methods on Bioactive Compounds and Antioxidant Capacity in Grape Skin Residues from the New Hybrid Variety "BRS Magna".

Molecules 2020 Aug 14;25(16). Epub 2020 Aug 14.

Department of Biotechnology, Health Science Institute, Federal University of Bahia (UFBA), Salvador 40170-115, Bahia, Brazil.

The effects of the drying process using the conventional oven and freeze-drying on the thermogravimetric profile, proximate composition, color parameters, individual bioactive compounds, and antioxidant activity in the grape residue (skin) were evaluated. Twenty individual phenolic compounds were identified, where a variation in concentration was observed for flavonols, stilbenes, phenolic acids, flavonoids, procyanidins, and particularly anthocyanins (malvidin-3,5-di--glucoside of 253.2-536.9 mg/kg) due to the drying process. Drying in a conventional oven caused a decrease of 23% of the total polyphenols. The skin of the BRS magna grape has a high concentration of total phenolic content of 489.5-148.3 mg.GAE/100 g, total anthocyanin content of 124.9-260.1 mg.CE/100 g, and total flavonoid content of 12.7-26.0 mg.QE/100 g. The results of free radical scavenging activity (1.26-4.91 μg/mL, as EC) and ferric reducing antioxidant power (82.93-184.10 μmol/g of skin as equivalent to FeSO) indicate high antioxidant activity, independently of the drying process applied. It was concluded that, if the application is directed to anthocyanin compounds, the use of lyophilization is recommended. On the other hand, if the interest is in bioactive compounds that exert antioxidant activity, conventional oven-drying can be used.
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http://dx.doi.org/10.3390/molecules25163701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466153PMC
August 2020

Grape peel (Syrah var.) jam as a polyphenol-enriched functional food ingredient.

Food Sci Nutr 2019 May 12;7(5):1584-1594. Epub 2019 Apr 12.

Federal University of Bahia (UFBA) Salvador Brazil.

In this study, we evaluated the effects of the storage time on the physicochemical properties, bioactive compound content, and antioxidant capacity of jam prepared from grape peel extract to explore its potential as a supplementary food and/or functional ingredient. The ethanolic extract from Syrah var. grape peel exhibited high bioactive compound concentrations and antioxidant activity. The jam stability (prepared with 8.9% of extract) at 14°C was evaluated at 0, 15, 30, 45, and 60 days. The jam was found to contain high concentrations of polyphenolic compounds (137.0 ± 3.2 mg of gallic acid equivalent/100 g), total flavonoids (128.5 ± 23.0 mg of equivalent/100 g), and total anthocyanins (92.5 ± 4.0 mg of cyanidin equivalent/100 g). However, a large reduction in the flavonoid (70%-90%), anthocyanin (29%-35%), and phenolic (23%-30%) content was observed during storage. The free radical-scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and β-carotene-linoleic acid assays revealed the great antioxidant potential of the jam prepared from grape peel extract, which exhibited significant levels of radical-neutralizing activity, especially as determined by the DPPH method with EC values ranging from 2.3 ± 0.1 to 3.9 ± 0.1 µg/ml. High values (> 0.90) were obtained for the correlation between the DPPH results and the concentrations of the compounds of interest. In summary, the high bioactive compound contents and antioxidant capacity of the jam produced from grape peel suggest that it may provide health benefits as a source of natural antioxidants upon incorporation to several food industry products.
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http://dx.doi.org/10.1002/fsn3.981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526673PMC
May 2019

Effects of photostimulation on the catabolic process of xenobiotics.

J Photochem Photobiol B 2019 Feb 12;191:38-43. Epub 2018 Dec 12.

Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Sciences, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA 40110-100, Brazil. Electronic address:

Light biotechnology is a promising tool for enhancing recalcitrant compounds biodegradation. Xenobiotics can cause a significant impact on the quality of the results achieved by sewage treatment systems due to their recalcitrance and toxicity. The optimization of bioremediation and industrial processes, aiming to increase efficiency and income is of great value. The aim of this study was to accelerate and optimize the hydrolysis of Remazol Brilliant Blue R by photo stimulating a thermophilic bacterial consortium. Three experimental groups were studied: control group; LED Group and Laser Group. The control group was exposed to the same conditions as the irradiated groups, except exposure to light. The samples were irradiated in Petri dishes with either a Laser device (λ660 nm, CW, θ = 0.04 cm2, 40 mW, 325 s, 13 J/cm2) or by a LED prototype (λ632 ± 2 nm, CW, θ = 0.5 cm2, 145 mW, 44 s, 13 J/cm2). We found that, within 48-h, statistically significant differences were observed between the irradiated and the control groups in the production of RNA, proteins, as well as in the degradation of the RBBR. It is concluded that, both Laser and LED light irradiation caused increased cellular proliferation, protein production and metabolic activity, anticipating and increasing the catabolism of the RBBR. Being the economic viability a predominant aspect for industrial propose our results indicates that photo stimulation is a low-cost booster of bioprocesses.
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http://dx.doi.org/10.1016/j.jphotobiol.2018.12.004DOI Listing
February 2019

Pilot-scale isolation and characterization of extracellular polymeric substances (EPS) from cell-free medium of Spirulina sp. LEB-18 cultures under outdoor conditions.

Int J Biol Macromol 2019 Mar 3;124:1106-1114. Epub 2018 Dec 3.

College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This objective of this work was to monitor the EPS production during the growth of Spirulina sp. LEB-18, evaluate the productivity and to characterize the exopolymers obtained on pilot-scale under outdoor conditions. The production of crude EPS occurred in all phases of biomass growth and was approximately ten folds higher than that biomass concentration of Spirulina sp. LEB-18 at the end cultivation, demonstrating the importance of the use of supernatant after harvesting of Spirulina to obtain high value bioproducts. The EPS extracted by Spirulina sp. LEB-18 are typically heteropolymers with one high molecular weight fraction (polysaccharides) with potential to be utilized as an alternative bioflocculant and another fraction of lower molecular mass (proteins). The presence of uronic acids, pyruvates and acyl groups of carbohydrates or carboxylic groups of amino acids in protein moiety is the main responsible for overall negative charge of EPS, which is also of biotechnological importance. Moreover, due to the pseudoplastic behavior of the solutions and high thermal stability, the obtained EPS can be widely applied in several industrial sectors, thus determining its technological and market potentiality.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.12.016DOI Listing
March 2019

Outdoor pilot-scale cultivation of Spirulina sp. LEB-18 in different geographic locations for evaluating its growth and chemical composition.

Bioresour Technol 2018 May 9;256:86-94. Epub 2018 Feb 9.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This study evaluated whether outdoor cultivation of Spirulina sp. in different geographical locations affected its growth and biomass quality, with respect to the chemical composition, volatile compound and heavy metal content, and thermal stability. The positive effect of solar radiation and temperature on biomass productivity in Spirulina sp. cultivated in the northeast was directly related to its improved nutritional characteristics, which occurred with an increase in protein, phycocyanin, and polyunsaturated fatty acid (mainly γ-linolenic) content. The biomass produced in Northeast and South Brazil showed high thermal stability and had volatile compounds that could be used as biomarkers of Spirulina, and their parameters were within the limits of internationally recognized standards for food additives; hence, they have been considered safe foods. However, the growth of crops in south Brazil occurred at lower rates due to low temperatures and luminous intensities, indicative of the robustness of microalgae in relation to these parameters.
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http://dx.doi.org/10.1016/j.biortech.2018.01.149DOI Listing
May 2018

New molecular features of cowpea bean (Vigna unguiculata, l. Walp) β-vignin.

Biosci Biotechnol Biochem 2018 Feb 17;82(2):285-291. Epub 2018 Jan 17.

b Department of Food, Environmental and Nutritional Sciences (DeFENS) , Università degli Studi di Milano , Milan , Italy.

Cowpea seed β-vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of β-vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.
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http://dx.doi.org/10.1080/09168451.2017.1419855DOI Listing
February 2018

Soy β-conglycinin (7S globulin) reduces plasma and liver cholesterol in rats fed hypercholesterolemic diet.

J Med Food 2011 Jan-Feb;14(1-2):94-100. Epub 2010 Dec 7.

Department of Food and Nutrition, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, São Paulo, Brazil.

The aim of this study was to examine the comparative hypocholesterolemic effect of soybean 7S fraction in rats fed a high-cholesterol diet. Soybean 7S globulin (β-conglycinin) was administered orally once a day to rats, and the effects were measured after 28 days. Wistar rats were divided into four groups: standard diet (STD) (casein alone), hypercholesterolemic (HC) diet (STD plus 1 g/100 g cholesterol and 0.5 g/100 g cholic acid), HC+7S(1) diet (HC diet plus 200 mg of 7S/kg of body weight/day), and HC+7S(2) diet (HC diet plus 300 mg of 7S/kg of body weight/day). Food intake, weight gain, animals' growth, and feeding efficiency ratio were similar among the STD and three HC groups, indicating that these parameters were not affected by treatments. Animals that had received different doses of soybean 7S globulin had lower total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL)/high-density lipoprotein (HDL) ratio in serum and lower levels of hepatic TC and TG than those fed only the HC diet. The atherogenic indexes of HC+7S(1) and HC+7S(2) groups were 40% and 55% lower than that of the HC group, respectively. The results showed that the oral daily administration of β-conglycinin in the diet to HC rats, at between 1.85% and 2.75% of total ingested protein, promotes the reduction of TC, LDL-cholesterol, and TG and an increase in HDL-cholesterol in the plasma, besides a small but significant reduction in cholesterol and TG levels in the liver of the animals as well as a reduced atherogenic index.
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http://dx.doi.org/10.1089/jmf.2009.0204DOI Listing
May 2011
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