Publications by authors named "E M O"

27 Publications

Establishes Commensalism in Germ-Free Mice Through the Reversible Downregulation of Virulence Gene Expression.

Front Immunol 2021 3;12:666088. Epub 2021 May 3.

Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.

The intestine harbors a complex community of bacterial species collectively known as commensal microbiota. Specific species of resident bacteria, as known as pathobiont, have pathogenic potential and can induce apparent damage to the host and intestinal inflammation in a certain condition. However, the host immune factors that permit its commensalism under steady state conditions are not clearly understood. Here, we studied the gut fitness of by using germ-free (GF) mice orally infected with this food-borne pathogen. persistently exists in the gut of GF mice without inducing chronic immunopathology. at the late phase of infection is not capable of infiltrating through the intestinal barrier. established the commensalism through the reversible down regulation of virulence gene expression. CD8 T cells were found to be sufficient for the commensalism of . CD8 T cells responding to contributed to the down-regulation of virulence gene expression. Our data provide important insights into the host-microbe interaction and have implications for developing therapeutics against immune disorders induced by intestinal pathogens or pathobionts.
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http://dx.doi.org/10.3389/fimmu.2021.666088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126713PMC
May 2021

Mucin degrader accelerates intestinal stem cell-mediated epithelial development.

Gut Microbes 2021 Jan-Dec;13(1):1-20

Mucosal Immunology Laboratory, Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with , a specialized species that degrades mucin. Administration of for 4 weeks accelerated the proliferation of Lgr5 ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of in the colon. The levels of acetic and propionic acids were higher in the cecal contents of -treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from -treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., ) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance.
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http://dx.doi.org/10.1080/19490976.2021.1892441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946046PMC
March 2021

Developing a novel diabetes distress e-learning program for diabetes educators: an intervention mapping approach.

Transl Behav Med 2021 06;11(6):1264-1273

School of Psychology, Deakin University, Geelong, Australia.

Diabetes distress is a common negative emotional response to the ongoing burden of living with diabetes. Elevated diabetes distress is associated with impaired diabetes self-management and quality of life yet rarely identified and addressed in clinical practice. Health professionals report numerous barriers to the provision of care for diabetes distress, including lack of skills and confidence, but few diabetes distress training opportunities exist. The purpose of this paper is to describe how we utilized Intervention Mapping to plan the development, implementation, and evaluation of a novel diabetes distress e-learning program for diabetes educators, to meet a well-documented need and significant gap in diabetes care. A multidisciplinary team (combining expertise in research, health and clinical psychology, diabetes education, nursing, tertiary education, and website architecture) developed a diabetes distress e-learning program. We followed a six-step process (logic model of the problem, program outcomes and objectives, program design, program production, program implementation plan, and evaluation plan) known as Intervention Mapping. The program is underpinned by educational and psychological theory, including Bloom's Taxonomy of Educational Objectives and social cognitive theory. We developed a short (estimated 4 h) e-learning program for diabetes educators, which draws on the content of the Diabetes and Emotional Health handbook and toolkit. It integrates a 7As model, which provides a stepwise approach to identifying and addressing diabetes distress. Our diabetes distress e-learning program has been developed systematically, guided by an Intervention Mapping approach. In the next phase of the project, we will trial the e-learning.
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http://dx.doi.org/10.1093/tbm/ibaa144DOI Listing
June 2021

Complex System for Monitoring the Patient's Condition and Diagnosis of Bronchial Asthma.

J Biomed Phys Eng 2020 Jun 1;10(3):367-374. Epub 2020 Jun 1.

MD, Department of Childhood Diseases No. 2, Rostov State Medical University, Rostov-on-Don, Russia.

In the present work, three versions of implementation of non-invasive bronchial asthma diagnostic system based on the author's technique of noninvasive diagnosis of bronchopulmonary diseases are considered. The offered variants of diagnostic system can be used both in medical institutions and at patient's home for the control of the patient's condition with the purpose of monitoring the dynamics of the disease during treatment, as well as for preventive purposes. Three variants of implementation of the diagnostic system with various complexity are considered. The results of a radiofrequency scanning of a human chest phantom with included heterogeneity simulating the presence of sputum in the human chest is a consequence of bronchial asthma.
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http://dx.doi.org/10.31661/jbpe.v0i0.1022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321396PMC
June 2020

Food antigens drive spontaneous IgE elevation in the absence of commensal microbiota.

Sci Adv 2019 05 22;5(5):eaaw1507. Epub 2019 May 22.

Academy of Immunology and Microbiology, Institute for Basic Science (IBS), Pohang, Republic of Korea.

Immunoglobulin E (IgE), a key mediator in allergic diseases, is spontaneously elevated in mice with disrupted commensal microbiota such as germ-free (GF) and antibiotics-treated mice. However, the underlying mechanisms for aberrant IgE elevation are still unclear. Here, we demonstrate that food antigens drive spontaneous IgE elevation in GF and antibiotics-treated mice by generating T helper 2 (T2)-skewed T follicular helper (T) cells in gut-associated lymphoid tissues (GALTs). In these mice, depriving contact with food antigens results in defective IgE elevation as well as impaired generation of T cells and IgE-producing cells in GALT. Food antigen-driven T cells in GF mice are mostly generated in early life, especially during the weaning period. We also reveal that food antigen-driven T cells in GF mice are actively depleted by colonization with commensal microbiota. Thus, our findings provide a possible explanation for why the perturbation of commensal microbiota in early life increases the occurrence of allergic diseases.
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http://dx.doi.org/10.1126/sciadv.aaw1507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531000PMC
May 2019
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