Publications by authors named "E E Buyko"

10 Publications

Role of HS in Regulation of Vascular Tone in Metabolic Disorders.

Bull Exp Biol Med 2021 Aug 20;171(4):431-434. Epub 2021 Sep 20.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

We studied the effect of the HS donor (NaHS, 1-500 μM) on the contractile responses of isolated aortic smooth muscle segments from rats with metabolic syndrome induced by high-fat, high-carbohydrate diet. It was found that the vasorelaxing effect of NaHS (5-100 μM) decreased in under conditions of MS. The endothelial NO synthase inhibitor L-NAME (100 μM) suppressed the effect of NaHS, while cystathionine-gamma-lyase inhibitor PAG (100 μM) decreased the vasodilating effects of acetylcholine (0.1-100 μM). Application of endogenous NO precursor L-arginine (1 mM) potentiated in the effects of HS donor NaHS. Thus, the contractile activity of vascular smooth muscles in metabolic syndrome is determined by not only the effect of HS, but also the influence of NO.
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http://dx.doi.org/10.1007/s10517-021-05243-yDOI Listing
August 2021

Anti-Adhesion Effect of Composite Film Materials Based on Glycoluril-Modified Sodium Carboxymethyl Cellulose.

Sovrem Tekhnologii Med 2021 28;13(1):35-40. Epub 2021 Feb 28.

Head of the Laboratory of Organic Synthesis, Research Office, National Research Tomsk State University, 36 Lenin Avenue, Tomsk, 634050, Russia.

was to develop composite film materials derived from modified sodium carboxymethyl cellulose and to evaluate their anti-adhesive effects.

Materials And Methods: The modified film materials were obtained by dissolving sodium carboxymethyl cellulose (Na-CMC) in an aqueous solution of a modifier (glycoluril) with subsequent homogenization and drying in a vacuum drying oven at room temperature. Physicomechanical parameters of the obtained films were determined using the Instron 3369 universal electromechanical testing machine (Great Britain) equipped with a climatic chamber (300-523 K), improved video extensometer, and the MKC-25 micrometer (Russia). Cytotoxicity of glycoluril-modified film materials derived from Na-CMC was studied by incubating cell cultures of 3T3-L1 mouse fibroblasts directly with extracts from films under study using a colorimetric test. Their anti-adhesion effect was investigated on 40 female Wistar rats by modeling a flat adhesion by inducing abrasion of the cecum and suturing the deserosed surface of the abdominal wall anatomically opposite the abrasion area. The presence of adhesions was assessed on day 8 after the operation. Commercial membrane Seprafilm (USA) was used as a reference sample.

Results: It was found that extracts obtained from film materials derived from Na-CMC modified with glycoluril at a concentration of 0.01 and 0.05 wt. % had no cytotoxic effect on the cell culture of mouse fibroblasts 3T3-L1. Flat adhesions were not detected when using Seprafilm. When film materials under study were placed in the abdominal cavity between the injured areas, formation of flat adhesions was not observed or observed in one case out of ten.

Conclusion: The obtained films are promising for preventing adhesions as a barrier-type agent. Modifying Na-CMC with glycoluril made it possible to create films that prevent formation of flat adhesions, have improved physical and mechanical properties and no cytotoxic effect.
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http://dx.doi.org/10.17691/stm2021.13.1.04DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353686PMC
February 2021

Regulation of Vascular Smooth Muscle Contractions in the Model of Metabolic Syndrome.

Bull Exp Biol Med 2020 Dec 2;170(2):196-199. Epub 2020 Dec 2.

Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.

Reduced glucose tolerance, hyperglycemia, and imbalance in lipid levels were found in rats with metabolic syndrome induced by a high-fat, high-carbohydrate diet. The contractile responses of intact and endothelium-denuded aortic smooth muscle segments from rats with metabolic syndrome to application of acetylcholine, phenylephrine, sodium nitroprusside, and forskolin were studied by mechanographic method. It was found that endothelial dysfunction develops against the background of metabolic and hemodynamic disorders in metabolic syndrome. It was shown that the regulation of vasoconstrictor reactions of vascular smooth muscles in metabolic syndrome is due to a decrease in Ca entry, mainly voltage-independent, as well as changes in the function of cGMP- and cAMP-activated K-channels.
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http://dx.doi.org/10.1007/s10517-020-05031-0DOI Listing
December 2020

Modification of titanium surface via Ag-, Sr- and Si-containing micro-arc calcium phosphate coating.

Bioact Mater 2019 Dec 2;4:224-235. Epub 2019 Aug 2.

Siberian State Medical University, Moscovskii Tract 2, Tomsk, 634050, Russia.

The current research is devoted to the study of the modification of the titanium implants by the micro-arc oxidation with bioactive calcium phosphate coatings containing Ag or Sr and Si elements. The coatings' microstructure, phase composition, morphology, physicochemical and biological properties were examined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). Ag-containing and Sr-Si-incorporated coatings were formed in alkaline and acid electrolytes, respectively. The formation of the coatings occurred at different ranges of the applied voltages, which led to the significant difference in the coatings properties. The trace elements Ag, Sr and Si participated intensively in the plasma-chemical reactions of the micro-arc coatings formation. Ag-containing coatings demonstrated strong antibacterial effect against AТСС 6538-P. MTT test with 3T3-L1 fibroblasts showed no cytotoxicity appearance on Sr-Si-incorporated coatings.
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http://dx.doi.org/10.1016/j.bioactmat.2019.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6684518PMC
December 2019

Active Learning-based corpus annotation--the PathoJen experience.

AMIA Annu Symp Proc 2012 3;2012:301-10. Epub 2012 Nov 3.

Jena University Language & Information Engineering (JULIE) Lab, Friedrich-Schiller-Universität Jena, Fürstengraben 30, D-07743 Jena, Germany.

We report on basic design decisions and novel annotation procedures underlying the development of PathoJen, a corpus of Medline abstracts annotated for pathological phenomena, including diseases as a proper subclass. This named entity type is known to be hard to delineate and capture by annotation guidelines. We here propose a two-category encoding schema where we distinguish short from long mention spans, the first covering standardized terminology (e.g. diseases), the latter accounting for less structured descriptive statements about norm-deviant states, as well as criteria and observations that might signal pathologies. The second design decision relates to the way annotation instances are sampled. Here we subscribe to an Active Learning-based approach which is known to save annotation costs without sacrificing annotation quality by means of a sample bias. By design, Active Learning picks up 'hard' to annotate instances for human annotators, whereas 'easier' ones are passed over to the automatic classifier whose models already incorporate and gradually improve with previous annotation experience.
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July 2013
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