Publications by authors named "E B Umoren"

6 Publications

Long-term consumption of -supplemented diet enhanced neurocognition, suppressed oxidative stress, acetylcholinesterase activity and neuronal degeneration in rat's hippocampus.

Drug Metab Pers Ther 2021 Mar 26. Epub 2021 Mar 26.

Neurophysiology Unit, Department of Physiology, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria.

Objectives: This study investigates protection against oxidative stress and memory enhancing potential of long-term consumption of leaves.

Methods: Male Wistar rat were fed with mixture of -supplemented diets (MOSD) partitioned in 1, 5, 10, and 20% continuously for 12 weeks. Object recognition test (ORT) and Morris water maze (MWM) was used for assessing neurocognition. Changes in body weight, Lipid peroxidation (MDA), Glutathione (GSH), Catalase (CAT) and Acetylcholinesterase (AChE) activity was assayed in the brain tissue. Histomorphometric of the hippocampus was also examined.

Results: The diets progressively increase the body weigh after the 12 weeks, improved spatial (MWM) and non-spatial (ORT) memory performance, protect against oxidative stress, inhibit AChE activity and suppresses neuronal degeneration in the hippocampus when stained with Cresyl violent stain.

Conclusions: Conclusively, long-term consumption of MOSD shows strong protection against oxidative stress and hippocampal degeneration and improves neurocognition with dose dependent effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/dmdi-2020-0189DOI Listing
March 2021

Synthetic Food dyes cause testicular damage via up-regulation of pro-inflammatory cytokines and down-regulation of FSH-R and TESK-1 gene expression.

JBRA Assist Reprod 2020 Nov 5. Epub 2020 Nov 5.

Department of Biochemistry, Faculty of Sciences, Madonna University, Nigeria.

Objective: This study investigated the effects of Tartrazine and Erythrosine (T+E) on the reproductive hormones and expression of some pro-inflammatory cytokines and testicular genes in testis of male Wistar rats.

Methods: 25 male Wistar rats (150-180g) were divided into 5 groups (n=5). Group 1 received distilled water while groups 2, 3, 4 and 5 were treated with T+E (2.5mg/kg, 5mg/kg, 10mg/kg and 20mg/kg) for the period of 23 days. Toxicity studies of the combined dye were investigated by evaluating serum reproductive hormones [Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Testosterone], gene expression and profiling, and testes histology.

Results: male Wistar rats (150-180g) were divided into 5 groups (n=5). Group 1 received distilled water while groups 2, 3, 4 and 5 were treated with T+E (2.5mg/kg, 5mg/kg, 10mg/kg and 20mg/kg) for the period of 23 days. Toxicity studies of the combined dye were investigated by evaluating serum reproductive hormones [Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Testosterone], gene expression and profiling, and testes histology.

Conclusions: This present study reveals that the dyes could impair testicular function as evident in the up-regulation of pro-inflammatory cytokines and down-regulation of TESK-1 gene expression and architecture of the testes leading to Orchitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5935/1518-0557.20200097DOI Listing
November 2020

Ulcerogenic and intestinal motility/transit stimulating actions of nevirapine in albino Wistar rats.

J Physiol Biochem 2013 Sep 28;69(3):547-57. Epub 2013 Mar 28.

Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

The antiretroviral is a non-nucleoside reverse transcriptase inhibitor of human immunodeficiency virus type 1. This study was undertaken to investigate the effect of nevirapine (NVP) administration on gastric acid secretion, pepsin secretion, mucosal secretion, intestinal motility, and transit using apparently healthy albino Wistar rats. Eighty albino Wistar rats (50-125 g body weight) from the start of the experiment were used for the study. Rats in the control group were fed normal rodent chow, while the NVP group was fed by gavage NVP (0.4 mg/kg body weight) two times daily (07:00 and 18:00 hours) in addition to normal rodent chow for 12 weeks. All animals were allowed free access to clean drinking water. Mean basal gastric output and peak acid output following histamine administration in the NVP-treated group were significantly higher (p < 0.001, respectively) compared to the control. Following cimetidine administration, there was significant decrease (p < 0.001) in peak acid output in the NVP-treated group compared to the control. The concentration of gastric pepsin, adherent mucus secretion, and mean value for ulcer score were significantly higher (p < 0.001) compared to their control group, respectively. There were significant increases (p < 0.05, respectively) in intestinal motility and basal contraction (p < 0.05) and increase in intestinal transit of the ileum of NVP-treated rats compared to their control, respectively. Results of the study suggest that NVP administration might provoke gastric ulceration in rats which may be caused by high pepsin, high basal acid output, and increased intestinal motility and transit.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13105-013-0243-xDOI Listing
September 2013

Chronic administration of the antiretroviral nevirapine increases body weight, food, and water intake in albino Wistar rats.

J Basic Clin Physiol Pharmacol 2012 ;23(2):89-92

Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

Background: Nevirapine (NVP) is an antiretroviral medication that prevents human immunodeficiency virus (HIV) cells from multiplying in the blood. This study was undertaken to investigate the effect of chronic administration of NVP on body weight, food, and water intake using apparently healthy albino Wistar rats.

Methods: Twenty adult albino Wistar rats (50-125 g body weight) were used for the study. Rats in the control group (n=10) were fed normal rodent chow, whereas the NVP group (n=10) were fed by gavage NVP (0.4 mg/kg body weight) two times daily (07.00 h and 18.00 h) in addition to normal rodent chow for 12 weeks. All animals were allowed free access to clean drinking water.

Results: Results showed that the mean daily food and water intake in the NVP group were significantly higher (p<0.001) when compared with the control group, respectively. The mean change in body weight in the NVP group was significantly higher (p<0.001) than the control group.

Conclusions: These results suggest that chronic administration of NVP may increase body weight in rats, probably due to its stimulatory effects on food and water intake.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/jbcpp-2012-0016DOI Listing
November 2012

The comparative effects of chronic consumption of kola nut (Cola nitida) and caffeine diets on locomotor behaviour and body weights in mice.

Niger J Physiol Sci 2009 Jun;24(1):73-8

Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria.

The comparative effects of chronic [28 days] consumption of kola nut and its active constituent, caffeine diets on locomotor behaviour and body weights in mice were investigated. Thirty adult Swiss white mice [15-30 g body weight], were used for the study. The open field-maze was employed for the evaluation of locomotor behaviour. Mice in the control group [n=10] were fed normal rodent chow, mice in the kola nut-fed group [n=10] were fed kola diet [25 % wt/wt of rodent chow] while those in the caffeine-fed group [n=10] were fed caffeine diet [0.66% wt/wt of rodent chow] for 4 weeks. All animals were allowed free access to clean drinking water. Daily food intake, water intake and body weight change were also measured. Daily food intake in the kola nut and caffeine-fed group of mice was significantly [P<0.001 respectively] lower than the control. There was also a significant [P<0.001] decrease in daily water intake in the caffeine-fed group compared to the control whereas, the apparent decrease of water intake in the kola nut-fed group was not significantly different from the control. Body weight change was also significantly [P<0.001 and P<0.05 respectively] lower in the kola nut and caffeine-fed groups of mice when compared to the control. The frequency of rearing in the open field was significantly [P<0.01] lower in the caffeine-fed group of mice when compared to the control. The frequency of grooming was also significantly [P<0.05] lower in the caffeine-fed group of mice when compared to the control. There was also a significant [P<0.05] decrease in the frequency of light-dark transitions in the light/dark transition box for the caffeine-fed group when compared to the control. The results showed that chronic consumption of kola nut and caffeine diets caused decrease in food intake and body weight. Consumption of caffeine-diet also significantly decreased water intake and locomotor activity. The effect of kola nut-diets on water intake and locomotor activity was not significant. Hence, the effect of kola nut on locomotor behaviour and water intake may not be due to caffeine only.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4314/njps.v24i1.46387DOI Listing
June 2009