Publications by authors named "Duygu Calik Kocaturk"

3 Publications

  • Page 1 of 1

Spectroscopic and microscopic comparisons of cell topology and chemistry analysis of mouse embryonic stem cell, somatic cell and cancer cell.

Acta Histochem 2021 Sep 29;123(6):151763. Epub 2021 Jul 29.

Ege University, Faculty of Medicine, Department of Histology and Embryology, 35100 Izmir, Turkey. Electronic address:

While embryonic stem cells and cancer cells are known to have many similarities in signalling pathways, healthy somatic cells are known to be different in many ways. Characterization of embryonic stem cell is crucial for cancer development and cancer recurrence due to the shared signalling pathways and life course with cancer initiator and cancer stem cells. Since embryonic stem cells are the sources of the somatic and cancer cells, it is necessary to reveal the relevance between them. The past decade has seen the importance of interdisciplinary studies and it is obvious that the reflection of the physical/chemical phenomena occurring on the cell biology has attracted much more attention. For this reason, the aim of this study is to elementally and topologically characterize the mouse embryonic stem cells, mouse lung squamous cancer cells, and mouse skin fibroblast cells by using Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Scanning Electron Microscopy (SEM) supported with Electron Dispersive Spectroscopy (EDS) techniques in a complementary way. Our AFM findings revealed that roughness data of the mouse embryonic stem cells and cancer cells were similar and somatic cells were found to be statistically different from these two cell types. However, based on both XPS and SEM-EDS results, surface elemental ratios vary in mouse embryonic stem cells, cancer cells and somatic cells. Our results showed that these complementary spectroscopic and microscopic techniques used in this work are very effective in cancer and stem cell characterization and have the potential to gather more detailed information on relevant biological samples.
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http://dx.doi.org/10.1016/j.acthis.2021.151763DOI Listing
September 2021

Holistic Approach to Cell Characteristics and Behavioral Analysis.

Crit Rev Oncog 2019 ;24(1):21-26

Department of Histology and Embryology, Ege University, Izmir, Turkey; Department of Histology and Embryology, Yüzüncü Yil University, Van, Turkey.

Cancer is a group of diseases of our era that affects not only medical status but also lowers the tone of social and emotional well-being. It also has severe impacts on economies. Cancer cells are linked to the somatic cells and stem cells, with their characteristic similarities and differences. Variations in cell-signaling pathways, such as cell death and proliferation balances, kinetic behavior of the cells, and their differentiation potentials, are the featured parameters for therapeutic targeting. The detection of target points should be based not only on the molecular biology methods but also on the physical and topological analyses from the aspect of "geography is fate". In this review, we focus on the importance of the holistic analyses of intracellular and extracellular dynamics and multidisciplinary cooperation.
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http://dx.doi.org/10.1615/CritRevOncog.2019029527DOI Listing
July 2020

The effect of extracellular matrix on the differentiation of mouse embryonic stem cells.

J Cell Biochem 2020 01 6;121(1):269-283. Epub 2019 Jun 6.

Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkey.

Embryonic stem cells (ESCs) are promising research materials to investigate cell fate determination since they have the capability to differentiate. Stem cell differentiation has been extensively studied with various microenvironment mimicking structures to modify cellular dynamics associated with the cell-extracellular matrix (ECM) interactions and cell-cell communications. In the current study, our aim was to determine the effect of microenvironmental proteins with different concentrations on the capacity and differentiation capability of mouse ESCs (mESCs), combining the biochemical assays, imaging techniques, Fourier transform infrared (FTIR) spectroscopy, and unsupervised multivariate analysis. Based on our data, coating the surface of mESCs with Matrigel, used as an acellular matrix substrate, resulted in morphological and biochemical changes. mESCs exhibited alterations in their phenotype after growing on the Matrigel-coated surfaces, including their differentiation capacity, cell cycle phase pattern, membrane fluidity, and metabolic activities. In conclusion, mESCs can be stimulated physiologically, chemically, or mechanically to convert them a new phenotype. Thus, identification of ESCs' behavior in the acellular microenvironment could be vital to elucidate the mechanism of diseases. It might also be promising to control the cell fate in the field of tissue engineering.
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http://dx.doi.org/10.1002/jcb.29159DOI Listing
January 2020
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