Publications by authors named "Duan Chenwang"

7 Publications

  • Page 1 of 1

Autologous fat grafting combined with negative pressure wound therapy in severe diabetic foot ulcer: a case study.

J Wound Care 2021 Apr;30(Sup4):S38-S40

Department of Burns and Plastic Surgery, Tangshan Gongren Hospital, China.

Objective: Hard-to-heal wounds are a surgical challenge, and diabetic foot ulcers (DFUs) are one of the most common and severe varieties. Previous studies have shown that autologous fat grafting (AFG) and negative pressure wound therapy (NPWT) have the potential to promote wound healing. This case study describes how these two methods together helped in the healing of a serious DFU.

Case History: A 65-year-old female patient had a severe DFU on her right foot, with a 30-year history of disease and renal failure. By the time symptoms were evident, regular dressing changes and antibiotic treatment were inadequate. She received surgical debridement, AFG and NPWT. Finally, as the granulation tissue covered the full wound bed, the wound was closed by split-thickness skin grafting. One month later, the DFU was fully healed with no recurrences.

Conclusion: The application of AFG or components of adipose tissue to treat hard-to-heal wounds has been researched at both the molecular level and in clinic. In this case, we have proved the curative effect of jointly using AFG and NPWT.
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http://dx.doi.org/10.12968/jowc.2021.30.Sup4.S38DOI Listing
April 2021

TLR2 affects CD86 expression and inflammatory response in burn injury mice through regulation of p38.

Biochem Cell Biol 2017 10 1;95(5):549-555. Epub 2017 May 1.

Department of Burn and Plastic Surgery, Tangshan Gongren Hospital, Tangshan 063000, Hebei, China.

The aim of this study was to assess the effects of TLR2-p38-CD86 signaling pathways on the inflammatory response in a mouse model of burn injury. Wild-type (TLR2) and mutant-type (TLR2) mice were obtained, and a mouse burn injury model was constructed. Tissue samples were examined with hematoxylin and eosin staining and the transferase mediated nick end labeling (TUNEL) method. Macrophages were treated with TLR2 agonist and p38 inhibitor. The expression levels of TLR2, p38, CD86, IL-1β, and TNF-α were quantified by RT-qPCR, Western blot, and ELISA. When compared with the sham group, the burn group had a significantly higher rate of apoptosis as well as higher expressions of TLR2, p38, CD86, IL-1β, and TNF-α. Inhibiting TLR2 was shown to significantly reduce the expressions of p-p38, CD86, IL-1β, and TNF-α. In the results of in-vitro experiments, TLR2 agonist increased the expression of p-p38, CD86, IL-1β, and TNF-α, whereas a p38 inhibitor was shown to reduce the expression of CD86, IL-1β, and TNF-α. Our results suggest that the TLR2-p38-CD86 signaling pathway plays a vital role in inflammation associated with burn injury.
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http://dx.doi.org/10.1139/bcb-2016-0210DOI Listing
October 2017

[Effect of botulinum toxin type A on excessive expansion of myocutaneous flap].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2011 Sep;25(9):1063-6

Department of Burn and Plastic Surgery, Tangshan Gongren Hospital, Tangshan Hebei 063000, PR China

Objective: To investigate the effects of botulinum toxin type A (BTXA) on the excessive expansion speed and blood supply of myocutaneous flap.

Methods: Seven adult Guizhou minipigs of clean grade were included, female or male and weighing 16-20 kg. The 2.4 mL BTXA solution (96 U) was injected in cutaneous muscle (24 points) of one side as experimental group (n = 7), the 2.4 mL saline in the other side as control group (n = 7). Two expanders (200 mL) were implanted beneath the cutaneous muscle on the bilateral flank of each pig symmetrically at 3 days after injection. One week later, the expanders were filled with saline every 4 days with an intracapsular pressure of 11.97 kPa, and accumulative total amounted to 400 mL for 3 weeks in control group and 5 weeks in experimental group. Then the expanders were taken out; the myocutaneous flaps formed and were sutured in situ. The myocutaneous flaps were cut for histological examination and capillary count. The expansion speed of the myocutaneous flap were recorded. The blood supply of the myocutaneous flap were observed by infrared thermography at 1 week after implantation expanders, before removing the expanders, and at 5 days after myocutaneous flap suture in situ.

Results: All the animals survived to the end of the experiment. The total expansion time was (54.0 +/- 3.1) days in experimental group and (67.0 +/- 3.9) days in control group, showing significant difference (t = -8.107, P = 0.000). All myocutaneous flaps survived after being sutured in situ. Infrared thermograph revealed that the temperature of the distal myocutaneous flap in experimental group was significantly higher than that in control group at 1 week after implantation of expanders (P < 0.05); at 5 days after myocutaneous flap suture in situ, the temperature of the central flap in experimental group was significantly higher than that in control group (P < 0.05); and there was no significant difference between 2 groups at the other time points (P > 0.05). The histological observation showed that the blood vessel density of the dermal layer and tissue between the capsule and the muscle layer in experimental group was significantly higher than those in control group (P < 0.05).

Conclusion: When excessive expansion is performed, BTXA can accelerate the expansion rate and improve the blood supply of expanded myocutaneous flaps.
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September 2011

Release within scar using the needle-scalpel for treatment of scar contracture of the neck.

Plast Reconstr Surg 2010 Apr;125(4):166e-167e

Second Plastic Surgery Department; Plastic Surgery Hospital; Chinese Academy of Medical Science; Peking Union Medical College; Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0b013e3181d45e03DOI Listing
April 2010

Application of botulinum toxin type A in myocutaneous flap expansion.

Plast Reconstr Surg 2009 Nov;124(5):1450-1457

Tangshan and Beijing, People's Republic of China From the Tangshan Worker's Hospital, Hebei Medical University; Beijing Hospital, Chinese Ministry of Health; Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Science; and No. 2 Hospital affiliated to General Hospital of the Chinese People's Liberation Army.

Background: Although the use of the expanded myocutaneous flap has many advantages, the time course is prolonged. The net gain in surface area during acute expansion is insufficient. In this study, botulinum toxin type A was applied to shorten the flap expansion period while obtaining an adequate surface area that would meet surgical requirements.

Methods: Seven minipigs were used for the authors' experiments. Two sides of the dorsolumbar section in each pig were divided randomly into the botulinum toxin type A-treated and the saline-treated groups. Two 200-ml expanders were implanted in the submuscular pocket. Inflation began 2 weeks later, and the period of expansion was observed in a double-blind manner. Two weeks after the final inflation, the expansion area was measured, and a 10 x 6-cm myocutaneous flap was elevated and sutured in situ, and the contraction of the myocutaneous flap was observed.

Results: Botulinum toxin type A shortened the expansion time by 17 days (p < 0.001). The average increment of effective expansion area (p = 0.009) and the average recruitment area (p = 0.001) in the botulinum toxin type A-treated group were significantly higher than in the saline-treated group. Moreover, contraction in axial length and width of the botulinum toxin type A-treated group was lower than that of the saline-treated group at each time point following transposition (p < 0.001).

Conclusions: Botulinum toxin type A could decrease the resistance to myocutaneous flap expansion, speed up the inflation, increase the expansion area, and reduce the contraction of the myocutaneous flap. It is a safe and convenient method with which to assist myocutaneous flap expansion.
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http://dx.doi.org/10.1097/PRS.0b013e3181b989beDOI Listing
November 2009

[Repair of hypospadias using scrotal septum vascular pedicle flap with two wing-like split-thickness skin graft].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009 Mar;23(3):316-8

Department of Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, 100144, PR China.

Objective: To investigate the effect of scrotal septum vascular pedicle flap with two wing-like split-thickness skin graft in reconstructing urethra in patients with hypospadias and in reducing the complications.

Methods: From January 2007 to March 2008, 30 cases of hypospadias were treated, with a median age of 10 years (2-20 years). There were 13 cases of proximal shaft type, 12 cases of penoscrotal type and 5 cases of scrotal type. There were 8 cases with operation history. The flap size ranged from 4.0 cm x 0.8 cm to 9.0 cm x 1.0 cm and 5 mm with two wing-like split-thickness skin graft. When the downward curvature corrected, length of the flap could be determined by the length of the urethra that needed to be reconstructed.

Results: All flaps survived. The incision healed by first intention in all patients except one whose incision healed after change dressing because of frequent erection. Incision at donor site healed well. Eighteen patients were followed up for 6-12 months, including 8 with second operation history. The reconstructed urethras were unobstructed and no urethrostenosis or urinary fistula happened.

Conclusion: This is an improved technique of the scrotal septum flap in urethra reconstruction. It takes advantages of both flap and split-thickness skin graft in reconstructing urethra and avoids the disadvantages of a single tissue. It is a good choice for treating hypospadias whose urinary meatus locates neighborly to penoscrotal junctional zone.
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March 2009

[Clinical application of autologous buccal micro-mucosa graft in vaginal reconstruction].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009 Mar;23(3):309-12

Second Department of Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100144, PR China.

Unlabelled: OBJECTIVE To investigate the effect of vaginal reconstruction with autologous buccal micro-mucosa graft.

Methods: From March 2007 and April 2008, 10 patients with absence of vagina were treated, aged 18-31 years (mean 26 years). Nine of them were congenital absence of vagina, and the remaining one was vaginal stenosis after vaginal reconstruction. They all exhibited normal secondary sexual characteristics, normal hormonal levels and 46, XX karyotype. Their abdominal ultrasonography revealed the normal ovaries and tubes but absence of the uterus or small rudimentary horns. However the one with vaginal stenosis had normal uterus. The buccal mucosa graft was minced into 0.5 mm in size and was transplanted to the cavity which was dissected between the bladder and the rectum.

Results: The operation was performed successfully in all cases. The operative time was about 1-2 hours and operative blood loss was 80-100 mL. Postoperative complication occurred in only one case for vaginal bleeding. The patient recovered and the wound healed well after immediate management. The others healed primarily without any complications. All cases were followed up for 4-16 months. The depth of neovagina which was formed was 6-10 cm and the width was about two fingers. The lining was pink-colored and smooth, and was confirmed as nonkeratinizing squamous stratified mucosa by histopathological examination. The donor sites healed uneventfully with no change in mouth opening. The perineal area was not disturbed. Four patients were married and satisfied with their sexual life without pain and bleeding.

Conclusion: Vaginal reconstruction with autologous buccal micro-mucosa graft is an easy, minimally invasive and useful method.
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March 2009