Publications by authors named "Doudou Chang"

3 Publications

  • Page 1 of 1

Metabolic regulation in soil microbial succession and niche differentiation by the polymer amendment under cadmium stress.

J Hazard Mater 2021 Aug 15;416:126094. Epub 2021 May 15.

Agricultural College, Shihezi University, Shihezi, Xinjiang 832000, PR China. Electronic address:

Cadmium (Cd) contamination seriously threatens the agricultural production, so exploring the response of soil microenvironment to amendments in Cd-contaminated soils is of importance. In this study, the mechanism of remediation of Cd-contaminated soil using the polymer amendment was studied in cotton flowering stage. The results showed that the concentration of Cd in cotton root and various Cd forms in Cd-contaminated soils were obviously high. High concentration of Cd, especially exchangeable Cd, could seriously affect the soil microenvironment. The root growth of cotton could be promoted, the carbon and nitrogen concentration and storage in soil were increased by 21.72-50.00%, while the exchangeable Cd concentration in soil were decreased by 41.43%, after applying the polymer amendment. In addition, the polymer amendment affected the soil microbial niche, increased the relative abundance of soil bacteria (Flaviaesturariibacter, Rubellimicrobium, and Cnuella), fungi (Verticillium and Tricharina), actinomycetes (Blastococcus and Nocardioides), and fungivores nematodes (Aphelenchus), and improved soil microbial metabolic functions (metabolism of nucleotides and carbohydrates). Therefore, this polymer amendment could be used to remediate severe Cd-contaminated soils, and the changes in the microbial and nematode communities help us understand the detoxification mechanism of the polymer amendment in Cd-contaminated soils.
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August 2021

Transgressive resistance to Heterodera glycines in chromosome segment substitution lines derived from susceptible soybean parents.

Plant Genome 2021 Jul 5;14(2):e20091. Epub 2021 Apr 5.

Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, Heilongjiang, 150081, China.

Chromosome segment substitution lines (CSSLs) are valuable genetic resources for quantitative trait loci (QTL) mapping of complex agronomic traits especially suitable for minor effect QTL. Here, 162 BC F -BC F CSSLs derived from crossing two susceptible parent lines, soybean [Glycine max (L.) Merr.] 'Suinong14' (recurrent parent) × wild soybean (G. soja Siebold & Zucc.) ZYD00006, were used for QTL mapping of soybean cyst nematode (SCN, Heterodera glycine Ichinohe) resistance based on female index (FI) and cysts per gram root (CGR) through phenotypic screening and whole-genome resequencing of CSSLs. Phenotypic results displayed a wide range of distribution and transgressive lines in both HG Type 2.5.7 FI and CGR and demonstrated a higher correlation between CGR and root weight (R = .5424) compared with than between FI and CGR (R = .0018). Using the single-marker analysis nonparametric mapping test, 33 significant QTL were detected on 18 chromosomes contributing resistance to FI and CGR. Fourteen QTL contributing 5.6-15.5% phenotypic variance (PVE) to FI were revealed on 11 chromosomes, and 16 QTL accounting for 6.1-36.2% PVE in CGR were detected on 14 chromosomes with strong additive effect by multiple-QTL model (MQM) mapping. Twenty-five and 13 out of all 38 QTL identified for FI and CGR on 20 chromosomes were from ZYD00006 and Suinong14, respectively. The CSSLs with the combination of positive alleles for FI, CGR, and root weight exhibited low nematode reproduction. For the first time, QTL associated with CGR have been detected, and both FI and CGR should be considered for breeding purposes in the absence of strong resistance genes such as rhg1 and Rhg4.
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July 2021

Application of compound material alleviates saline and alkaline stress in cotton leaves through regulation of the transcriptome.

BMC Plant Biol 2020 Oct 8;20(1):462. Epub 2020 Oct 8.

Agricultural College, Shihezi University, Shihezi, Xinjiang, 832000, People's Republic of China.

Background: Soil salinization and alkalinization are the main factors that affect the agricultural productivity. Evaluating the persistence of the compound material applied in field soils is an important part of the regulation of the responses of cotton to saline and alkaline stresses.

Result: To determine the molecular effects of compound material on the cotton's responses to saline stress and alkaline stress, cotton was planted in the salinized soil (NaCl 8 g kg) and alkalized soil (NaCO 8 g kg) after application of the compound material, and ion content, physiological characteristics, and transcription of new cotton leaves at flowering and boll-forming stage were analyzed. The results showed that compared with saline stress, alkaline stress increased the contents of Na, K, SOD, and MDA in leaves. The application of the compound material reduced the content of Na but increased the K/Na ratio, the activities of SOD, POD, and CAT, and REC. Transcriptome analysis revealed that after the application of the compound material, the Na/H exchanger gene in cotton leaves was down-regulated, while the K transporter, K channel, and POD genes were up-regulated. Besides, the down-regulation of genes related to lignin synthesis in phenylalanine biosynthesis pathway had a close relationship with the ion content and physiological characteristics in leaves. The quantitative analysis with PCR proved the reliability of the results of RNA sequencing.

Conclusion: These findings suggest that the compound material alleviated saline stress and alkaline stress on cotton leaves by regulating candidate genes in key biological pathways, which improves our understanding of the molecular mechanism of the compound material regulating the responses of cotton to saline stress and alkaline stress.
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October 2020