Publications by authors named "Dongsheng Zhang"

277 Publications

Role of soft bi-layer coating on the protection of turtle carapace.

J Biomech 2021 Jul 9;126:110618. Epub 2021 Jul 9.

Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai Institute of Applied Mathematics and Mechanics, School of Mechanics and Engineering Science, Shanghai University, Shanghai 200444, China.

The turtle carapace is a biological armor exhibiting enhanced protection performance. Despite considerable efforts to characterize the structure-property relations of the turtle carapace, how the design of soft keratin-collagen bi-layer coating contributes to the protection of this biological armor remains largely unknown. In this study, calculations are carried out for fracture of the turtle carapace subjected to impact loading. The dynamic fracture of the bone layer, plastic deformation of the keratin-collagen bi-layer, and delamination at the keratin-collagen and collagen-bone interfaces are accounted for in the analyses. We reveal that plastic deformation and interfacial delamination within the soft bi-layer coating are two toughening mechanisms controlling the resistance to dynamic crack growth in the bone layer of the turtle carapace. The architecture of the keratin-collagen bi-layer coating enables large plastic deformation in the collagen layer and multiple delaminations within the bi-layer coating, preventing crack propagation in the bone layer. It is found that the dynamic fracture of bone layer in the turtle carapace depends on the stiffness mismatch and yield stress contrast between the keratin layer and the collagen layer. As the stiffness mismatch increases, small plastic deformation of the bi-layer coating occurs and the plastic deformation of collagen layer tends to emerge in the vicinity of the keratin-collagen interface, suppressing interfacial delamination and leading to weak resistance to fracture of the bone layer. The intermediate level of yield stress contrast can activate large plastic deformation and multiple delaminations within the bi-layer coating, mitigating fracture of the bone layer.
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http://dx.doi.org/10.1016/j.jbiomech.2021.110618DOI Listing
July 2021

Description of three species of ophioplinthacids, including a new species, from a deep seamount in the Northwest Pacific Ocean.

PeerJ 2021 2;9:e11566. Epub 2021 Jul 2.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, China.

Five specimens of brittle star were collected from a deep-sea seamount in the Northwest Pacific, and identified into three species. One which is new to science, n. sp., can be easily distinguished from its congeners by the distinctly elongated and stout tentacle scales, stout and long disc spines, capitate with typically elongate to flaring head bearing numerous distinct thorns, radial shields roughly triangular and contiguous distally. One specimen was identified as (Clark, 1949), which had been reported in Hawaii seamounts, is a new record of this species in the Northwest Pacific. The remaining specimen was an unknown species of , with some different characteristics from other species of . However, we, herein, prefer not to attach a name to this specimen until more morphological characteristics are available. The finding of this new species and two new records further enriches the distribution of in the seamount of Northwest Pacific, providing useful information for marine protection in the cobalt-rich area.
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http://dx.doi.org/10.7717/peerj.11566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256812PMC
July 2021

Development and analytical validation of a novel bioavailable 25-hydroxyvitamin D assay.

PLoS One 2021 9;16(7):e0254158. Epub 2021 Jul 9.

Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America.

Background: Bioavailable 25-hydroxyvitamin D (25OHD) may be a better indicator of vitamin D sufficiency than total 25OHD. This report describes a novel assay for measuring serum bioavailable 25OHD.

Methods: We developed an assay for 25OHD % bioavailability based on competitive binding of 25OHD tracer between vitamin D-binding protein (DBP)-coated affinity chromatography beads and serum DBP. Bioavailable 25OHD, total 25OHD, albumin, and DBP protein concentrations were measured in 89 samples from hospitalized patients and 42 healthy controls to determine how the DBP binding assay responds to differences in concentrations of DBP and compares to calculated bioavailable 25OHD values.

Results: DBP binding assay showed a linear relationship between DBP-bound 25OHD tracer recovered from bead supernatant and DBP calibrator concentrations (y = 0.0017x +0.731, R2 = 0.9961, p<0.001). Inversion of this relationship allowed interpolation of DBP binding equivalents based upon 25OHD tracer recovered. The relationship between DBP binding equivalents and % bioavailability fits a non-linear curve, allowing calculation of % bioavailable 25OHD from DBP binding equivalents (y = 10.625x-0.817, R2 = 0.9961, p<0.001). In hospitalized patient samples, there were linear relationships between DBP protein concentrations and DBP binding equivalents (y = 0.7905x + 59.82, R2 = 0.8597, p<0.001), between measured vs. calculated % bioavailability (y = 0.9528 + 0.0357, R2 = 0.7200, p<0.001), and between absolute concentrations of measured vs. calculated bioavailable 25OHD (y = 1.2403 + 0.1221, R2 = 0.8913, p<0.001).

Conclusions: The DBP-binding assay for bioavailable 25OHD shows expected changes in 25OHD % bioavailability in response to changes in DBP concentrations and concordance with calculated bioavailable 25OHD concentrations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254158PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270209PMC
July 2021

LncRNA SNHG14 promotes cell proliferation and invasion in colorectal cancer through modulating miR-519b-3p/DDX5 axis.

J Cancer 2021 11;12(16):4958-4970. Epub 2021 Jun 11.

Department of General Surgery, The First Affiliated Hospital Of Nanjing Medical University, Nanjing, Jiangsu Province, China.

Numbers of studies suggest that long non-coding RNAs (lncRNAs) exert an important role in cancer progression. It is reported that lncRNA SNHG14 (SNHG14) promotes cell proliferation and invasion in many cancers. However, the underlying molecular mechanism of SNHG14 in colorectal cancer (CRC) remains unclear. In our study, we found that SNHG14 is highly expressed in CRC tissues and cells, especially in SW480 and HT-29 cells. In addition, sh-SNHG14 inhibits cell proliferation, cell migration and invasion, promotes cell apoptosis in CRC cell lines. Furthermore, we found that SNHG14 functions as a sponge for miR-519b-3p, while the DEAD box protein 5 (DDX5) is a downstream target gene of miR-519b-3p, and the functions of miR-519b-3p inhibitors on the CRC progression could be rescued by downregulation of DDX5. Our findings suggest that SNHG14 promotes the CRC progression by miR-519b-3p/DDX5 axis, implying the promising therapeutic target of SNHG4 for CRC patients.
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http://dx.doi.org/10.7150/jca.55495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247390PMC
June 2021

Altered Functional Connectivity of Insular Subregions in Type 2 Diabetes Mellitus.

Front Neurosci 2021 16;15:676624. Epub 2021 Jun 16.

Department of MRI, Shaanxi Provincial People's Hospital, Xi'an, China.

Diabetes-related brain damage can lead to cognitive decline and increase the risk of depression, but the neuropathological mechanism of this phenomenon remains unclear. Different insular subregions have obvious functional heterogeneity, which is related to many aspects of type 2 diabetes mellitus (T2DM)-related brain damage. However, little is known about changes in functional connectivity (FC) in insular subregions in patients with T2DM. Therefore, we aimed to investigate FC between different insular subregions and clinical/cognitive variables in patients with T2DM. Fifty-seven patients with T2DM and 55 healthy controls (HCs) underwent a neuropsychological assessment and resting-state FC examination. We defined three insular subregions, including the bilateral dorsal anterior insula (dAI), bilateral ventral anterior insula (vAI), and bilateral posterior insula (PI). We examined differences in FC between insular subregions and the whole brain in patients with T2DM compared with HCs. A correlation analysis was performed to examine the relationship between FC and clinical/cognitive variables. Compared with HCs, patients with T2DM showed significantly decreased FC between the dAI and the right inferior frontal gyrus, right superior/middle temporal gyrus, right hippocampus, and right precentral gyrus. FC between the vAI and the right supramarginal gyrus, as well as the PI and the right precentral/postcentral gyrus, was reduced in the T2DM group compared with the control group. In the T2DM group, we showed a significant negative correlation between glycated hemoglobin concentration and FC in the dAI and right hippocampus ( = -0.428, = 0.001) after Bonferroni correction. We conclude that different insular subregions present distinct FC patterns with functional regions and that abnormal FC in these insular subregions may affect cognitive, emotional, and sensorimotor functions in patients with T2DM.
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http://dx.doi.org/10.3389/fnins.2021.676624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242202PMC
June 2021

Right Frontoinsular Cortex: A Potential Imaging Biomarker to Evaluate T2DM-Induced Cognitive Impairment.

Front Aging Neurosci 2021 28;13:674288. Epub 2021 May 28.

Department of MRI, Shaanxi Provincial People's Hospital, Xi'an, China.

Cognitive impairment in type 2 diabetes mellitus (T2DM) is associated with functional and structural abnormalities in the intrinsic brain network. The salience network (SN) is a neurocognitive network that maintains normal cognitive function, but it has received little attention in T2DM. We explored SN changes in patients with T2DM with normal cognitive function (DMCN) and in patients with T2DM with mild cognitive impairment (DMCI). Sixty-five T2DM patients and 31 healthy controls (HCs) underwent a neuropsychological assessment, independent component analysis (ICA), and voxel-based morphometry (VBM) analysis. The ICA extracted the SN for VBM to compare SN functional connectivity (FC) and gray matter (GM) volume (GMV) between groups. A correlation analysis examined the relationship between abnormal FC and GMV and clinical/cognitive variables. Compared with HCs, DMCN patients demonstrated increased FC in the left frontoinsular cortex (FIC), right anterior insula, and putamen, while DMCI patients demonstrated decreased right middle/inferior frontal gyrus FC. Compared with DMCN patients, DMCI patients showed decreased right FIC FC. There was no significant difference in SN GMV in DMCN and DMCI patients compared with HCs. FIC GMV was decreased in the DMCI patients compared with DMCN patients. In addition, right FIC FC and SN GMV positively correlated with Montreal Cognitive Assessment and Mini-Mental State Examination (MMSE) scores. These findings indicate that changes in SN FC, and GMV are complex non-linear processes accompanied by increased cognitive dysfunction in patients with T2DM. The right FIC may be a useful imaging biomarker for supplementary assessment of early cognitive dysfunction in patients with T2DM.
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http://dx.doi.org/10.3389/fnagi.2021.674288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193040PMC
May 2021

circMTO1 sponges microRNA-219a-5p to enhance gallbladder cancer progression via the TGF-β/Smad and EGFR pathways.

Oncol Lett 2021 Jul 27;22(1):563. Epub 2021 May 27.

Department of Hepatobiliary Surgery, Lanzhou No. 2 People's Hospital, Lanzhou, Gansu 730046, P.R. China.

Circular mitochondrial translation optimization 1 homologue (circMTO1) has been reported to regulate the tumorigenesis of different types of cancer; however, the role of circMTO1 in gallbladder cancer (GBC) remains unknown. The present study aimed to identify the potential miRNAs and target genes of circMTO1 during GBC progression, and clarify the regulatory mechanism between circMTO1 and miRNAs or target genes. The present study performed MTT and Transwell assays, and Annexin V staining to assess cell viability, migration and apoptosis, respectively. In addition, a lymphatic vessel formation assay was performed to assess tube formation of human dermal lymphatic endothelial cells (HDLECs), and GBC-SD and NOZ cells. The results demonstrated that circMTO1 knockdown significantly attenuated the viability and migration of GBC cells and tube formation of HDLECs, and promoted apoptosis, indicating a tumor-promoting role of circMTO1. In addition, transfection with microRNA (miRNA/miR)-219a-5p inhibitor rescued short hairpin RNA-circMTO1-inhibited tumorigenesis of GBC cells, suggesting that miR-219a-5p acts as a downstream effector for circMTO1. Mechanistically, transfection with miR-219a-5p mimic suppressed the expression levels of Smad2/4 and epidermal growth factor receptor. Analysis of The Cancer Genome Atlas datasets revealed that circMTO1 expression was associated with overall survival and the stage of patients with GBC. Taken together, the results of the present study provide novel insight for the role of circMTO1-induced GBC tumorigenesis via regulation of miR-219a-5p expression.
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http://dx.doi.org/10.3892/ol.2021.12824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185704PMC
July 2021

Refractive three-dimensional reconstruction for underwater stereo digital image correlation.

Opt Express 2021 Apr;29(8):12131-12144

Measuring the three-dimensional (3D) deformation of submerged objects through different media with the stereo digital image correlation (stereo-DIC) method involves the refractive optical imaging problem where the non-linear transmission of light is induced by a change of medium density. The problem invalidates the underlying single viewpoint assumption of the perspective model in regular stereo-DIC, thereby resulting in erroneous measurements of 3D shape and deformation. In this work, we propose a refractive stereo-DIC method that overcomes the problem by considering light refraction in 3D reconstruction. We formulate a full refractive reconstruction geometry description based on Snell's law of flat refraction and the regular triangulation. This allows the true shape to be effectively reconstructed by tracing and establishing the refracted ray-paths based on the regular 3D reconstruction, without reformulating the camera model and image formation. The refractive stereo-DIC is finally established by integrating the refractive 3D reconstruction into the regular DIC framework for measuring accurate 3D shape and deformation of submerged objects. We experiment the proposed approach with underwater 3D shape and deformation measurements. Both results prove its feasibility and correctness, further heralding our approach as a flexible solution that could readily extend the stereo-DIC to fluid-immersed 3D deformation characterization.
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http://dx.doi.org/10.1364/OE.421708DOI Listing
April 2021

First report of fatal baylisascariasis-induced acute pancreatitis in a giant panda.

Parasitol Int 2021 May 10;84:102380. Epub 2021 May 10.

Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan Academy of Giant Panda, Chengdu, Sichuan, China.

A wild adult male giant panda that was rescued from a nature reserve in Sichuan Province, China, has died. The panda had been in poor physical condition: it was wheezing and had increased serum amylase. A pathological examination was performed in order to determine the cause of death. Gross examination revealed 1380 mL of yellowish fluid in the abdominal cavity, 356 nematodes in the digestive tract and one filling the pancreatic duct, contractions and variably-sized dark purple areas in the spleen, a collapsed right lung and consolidation of the left lung. Acute pancreatitis was confirmed histopathologically via edema, focal necrosis and hemorrhage with inflammatory cell infiltration. Other major histopathological changes included serous-hemorrhagic pneumonia, lymphocytic necrosis and depletion in the spleen, and degeneration and necrosis of renal tubular epithelial cells. The nematodes were identified as Baylisascaris schroederi via molecular assays. In conclusion, the cause of death of the giant panda was determined to be multiple organ dysfunction syndrome caused by baylisascariasis-induced acute pancreatitis. To our knowledge, this is the first report of fatal baylisascariasis-induced acute pancreatitis in the giant panda.
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http://dx.doi.org/10.1016/j.parint.2021.102380DOI Listing
May 2021

Minimally invasive surgery rib fracture fixation based on location and anatomical landmarks.

Eur J Trauma Emerg Surg 2021 May 13. Epub 2021 May 13.

Department of Cardiothoracic Surgery, Shijiazhuang Third Hospital, No. 15 Tiyu South Street, Shijiazhuang, 050011, Hebei, China.

Background: Rib fracture is closely related to thoracic injury with high morbidity and mortality. This study aimed to investigate the clinical effect of Zhang ZhiFei (ZZF) zoning method on the selection of incision and approach in minimally invasive surgery for rib fracture.

Methods: A total of 110 patients with rib fractures from July 2017 to July 2019 were enrolled in the study. Preoperative computed tomography and three-dimensional reconstruction of ribs was performed. Then, the rib fractures to be surgically fixed were divided into costal cartilage zone, chest zone, lateral costal zone, high posterior costal zone, low posterior costal subscapular zone, and low posterior costal paraspinal zone, which was called ZZF zoning method. Rib fractures in each zone had unique minimally invasive incision approach, and the open reduction and internal fixation of rib fracture was performed under minimally invasive surgery of corresponding small incision.

Results: The average incision length and number of incisions of the 110 patients were 6.2 cm and 1.3, respectively. The average number of internal fixation was 5.3 and the average operation time was 82 min. The postoperative fracture end was well aligned. After 3 months of follow-up, no internal fixation was displaced or detached.

Conclusion: Based on the anatomical characteristics of different zones of the chest wall, ZZF zoning method provides a new idea and reference for the selection of incision and approach in minimally invasive internal fixation for rib fracture.
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http://dx.doi.org/10.1007/s00068-021-01676-2DOI Listing
May 2021

Simvastatin intervention mitigates hypercholesterolemia-induced alveolar bone resorption in rats.

Exp Ther Med 2021 Jun 15;21(6):628. Epub 2021 Apr 15.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Shangdong University and Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.

Simvastatin promotes bone formation and increases bone mineral density in patients with hyperlipidemia and ameliorates hypercholesterolemia-induced microstructure changes in the jaw bone of animals. However, whether and how treatment with simvastatin can modulate the hypercholesterolemia-induced alveolar bone resorption is unclear. The present study aimed to examine the therapeutic efficacy and potential mechanisms of simvastatin application in hypercholesterolemia-induced alveolar bone resorption. The association between hyperlipidemia and alveolar bone resorption in 100 patients with periodontitis was examined. Additionally, male Sprague-Dawley rats were fed a standard rodent chow (NC) for 32 weeks or a high cholesterol diet (HCD) for 24 weeks. The HCD-fed rats were randomized, continually fed with HCD and treated with vehicle saline (HC) or simvastatin by gavage (5 mg/kg; SIM, n=10/group) for 8 weeks. The morphological changes to alveolar bone resorption in rats were analyzed by linear measurements. The relative levels of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand RANKL, nuclear factor-κB (NF-κB), microtubule-associated protein 1 light chain 3 (LC3) and p62 in the alveolar bone tissues were determined by reverse transcription-quantitative PCR and/or immunohistochemistry. Sulcus bleeding index (SBI), clinical attachment loss (CAL), probing depth (PD) and the distance of cemantoenamel junction-alveolar bone crest (CEJ-ABC) in patients with hyperlipidemia were significantly greater than that in the controls (P<0.001). The levels of hyperlipidemia were positively correlated with the values of SBI, CAL, PD and CEJ-ABC in this population. Compared with the NC rats, higher levels of alveolar bone resorption, NF-κB expression, higher ratios of RANKL/OPG mRNA transcripts and LC3 to p62 expression were detected in the alveolar bone tissues of HC group. Simvastatin intervention significantly mitigated hypercholesterolemia-induced alveolar bone loss and RANKL mRNA transcription, but increased the ratios of LC3/p62 protein expression in the alveolar bone tissues of rats. Hyperlipidemia is associated with alveolar bone resorption and simvastatin treatment alleviated the hypercholesterolemia-related alveolar bone loss by down-regulating the NF-κB expression.
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http://dx.doi.org/10.3892/etm.2021.10060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082588PMC
June 2021

Exogenous transforming growth factor-β1 prevents the inflow of fluoride to ameleoblasts through regulation of voltage-gated chloride channels 5 and 7.

Exp Ther Med 2021 Jun 14;21(6):615. Epub 2021 Apr 14.

Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.

Dental fluorosis is a global issue. Although there are multiple causes of dental fluorosis, the precise mechanism remains controversial. Previous studies have demonstrated that extracellular fluoride may promote an accumulation of fluoride ions in ameloblasts, which may induce oxidative and endoplasmic reticulum stresses, leading to dental fluorosis. However, the exact process by which fluoride ions enter cells has not been determined. In the present study, intracellular fluoride concentration was determined using a newly developed specific fluorescent probe called probe 1. Under high extracellular fluoride concentrations, the fluorescence intensity of the ameloblasts increased, however, exogenous transforming growth factor-β1 (TGF-β1) was able to inhibit the increase. Furthermore, changes in the expression of the voltage-gated chloride channels 5 and 7 (ClC5 and ClC-7), which are responsible for the transport of fluoride were investigated. The results indicated that fluoride reduced the expression of endogenous TGF-β1 and increased the expression of ClC-5 and ClC-7. Additionally, exogenous TGF-β1 reduced the expression of ClC-5 and ClC-7. The results of the present study indicate that exogenous TGF-β1 may prevent accumulation of fluoride in ameloblasts through the regulation of ClC-5 and ClC-7 under high extracellular fluoride concentrations.
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http://dx.doi.org/10.3892/etm.2021.10047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082615PMC
June 2021

Comprehensive analysis of immune-related prognostic genes in the tumour microenvironment of hepatocellular carcinoma.

BMC Cancer 2021 Mar 31;21(1):331. Epub 2021 Mar 31.

Department of Hepatobiliary and Pancreatic Surgery, Qingdao Municipal Hospital Affiliated to Qingdao University, Qingdao, Shandong Province, 266000, People's Republic of China.

Background: The mortality rate of hepatocellular carcinoma (HCC) remains high worldwide despite surgery and chemotherapy. Immunotherapy is a promising treatment for the rapidly expanding HCC spectrum. Therefore, it is necessary to further explore the immune-related characteristics of the tumour microenvironment (TME), which plays a vital role in tumour initiation and progression.

Methods: In this research, 866 immune-related differentially expressed genes (DEGs) were identified by integrating the DEGs of samples from The Cancer Genome Atlas (TCGA)-HCC dataset and the immune-related genes from databases (InnateDB; ImmPort). Afterwards, 144 candidate prognostic genes were defined through weighted gene co-expression network analysis (WGCNA).

Results: Seven immune-related prognostic DEGs were identified using the L1-penalized least absolute shrinkage and selection operator (LASSO) Cox proportional hazards (PH) model, and the ImmuneRiskScore model was constructed on this basis. The prognostic index of the ImmuneRiskScore model was then validated in the relevant dataset. Patients were divided into high- and low-risk groups according to the ImmuneRiskScore. Differences in the immune cell infiltration of patients with different ImmuneRiskScore values were clarified, and the correlation of immune cell infiltration with immunotherapy biomarkers was further explored.

Conclusion: The ImmuneRiskScore of HCC could be a prognostic marker and can reflect the immune characteristics of the TME. Furthermore, it provides a potential biomarker for predicting the response to immunotherapy in HCC patients.
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http://dx.doi.org/10.1186/s12885-021-08052-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011181PMC
March 2021

CircRNA circ_0124554 blocked the ubiquitination of AKT promoting the skip lymphovascular invasion on hepatic metastasis in colorectal cancer.

Cell Death Dis 2021 03 15;12(3):270. Epub 2021 Mar 15.

Colorectal Surgery Division, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.

Colorectal cancer (CRC) is the fourth most common cancer in men and the third most common cancer in women worldwide. The incidence and mortality of CRC was increasing rapidly in China. Lymph node-negative colorectal cancer patients with synchronous liver metastasis (LNLM1) was defined as "skip" lymph vascular invasion on hepatic metastasis, who presenting poor prognosis. We aiming to investigate the potential mechanism for the "skip" lymph vascular invasion on hepatic metastasis in colorectal cancer. The microarray was applied for screening the transcription landscape of circRNA in lymph node negative CRC patients with synchronous liver metastasis (LNLM1) or without liver metastasis (LNLM0). We identified the aberrant increased circRNA circ_0124554 (also entitled as circ-LNLM) in tumor tissues of LNLM1 patients comparing with either the tumor tissues of LNLM0 or adjacent tissues of LNLM1. Circ-LNLM1 expression was highly correlated with liver metastasis and vascular invasion. Ectopic expression of cytoplasmic located circ-LNLM could promote invasion of CRC cells and induced the liver metastasis in animal models through the direct binding with AKT. The phosphorylation of AKT (T308/S473) was activated due to the blocked ubiquitination site of Lys in 0-52aa peptide of circ-LNLM. Endogenous plasma expression of circ-LNLM induced poor prognosis of LNLM1 and could distinguish LNLM1 patients from LNLM0. In conclusion, the circ-LNLM blocked the ubiquitination of AKT could promote the early metastasis especially for the lymph node-negative colorectal cancer patients with synchronous liver metastasis. The circ-LNLM might be prognosis and diagnosis biomarker for LNLM1 patients.
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http://dx.doi.org/10.1038/s41419-021-03565-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960696PMC
March 2021

Real World First-Line Treatments and Outcomes of Nab-Paclitaxel Plus Gemcitabine, mFOLFIRINOX and GEMOX in Unresectable Pancreatic Cancer from a Chinese Single Institution.

Curr Oncol 2020 12 30;28(1):209-219. Epub 2020 Dec 30.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, China.

Background: There have not been any head-to-head prospective studies to compare the effects of different chemotherapy regimens as first-line treatments for unresectable pancreatic cancer (UPC). We aimed to compare the effectiveness of nab-paclitaxel plus gemcitabine, mFOLFIRINOX and gemcitabine plus oxaliplatin (GEMOX) as first-line treatments by using real-world data from Chinese patients.

Methods: We retrospectively included patients with UPC treated with nab-paclitaxel plus gemcitabine, mFOLFIRINOX or GEMOX as a first-line treatment at Sun Yat-sen University Cancer Center. Overall survival (OS), progression-free survival (PFS), objective response rate (ORR) and disease control rate (DCR) were assessed.

Results: A total of 117 patients were administered nab-paclitaxel plus gemcitabine ( = 62), mFOLFIRINOX ( = 30) or GEMOX ( = 25) as first-line chemotherapy. The median OS was 11.1, 10.1 and 10.2 months ( = 0.75) in the nab-paclitaxel plus gemcitabine, mFOLFIRINOX and GEMOX, respectively. The ORR was similar among the three groups (24%, 23% and 32%, = 0.76) and the DCR was higher in the nab-paclitaxel-gemcitabine group (82%) than the other two groups (60% and 64%, = 0.04). The most common adverse events of grade 3 or 4 were neutropenia (32%, 28% and 5%), peripheral neuropathy (13%, 16% and 0) and fatigue (9%, 16% and 5%). Febrile neutropenia occurred in 2%, 4% and 5% of the patients in the three groups.

Conclusion: In the first line treatment of UPC, our results suggest that nab-paclitaxel plus gemcitabine was associated with a higher DCR than mFOLFIRINOX or GEMOX, while all groups demonstrated similar OS, PFS and ORR.
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http://dx.doi.org/10.3390/curroncol28010023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816170PMC
December 2020

Dual-lens system in enlarging the viewing angle for the Michelson interferometer.

Appl Opt 2021 Feb;60(6):1529-1534

Shearography has been widely accepted as a non-destructive evaluation tool in engineering. However, the field of view is usually limited due to the use of the Michelson optical arrangement if the working distance is strictly constrained. In order to improve efficiency, we propose a dual-lens system to expand the view angle of the Michelson optical arrangement. Two imaging lenses, which are entitled as the objective lens and camera lens, are placed in front of and behind the Michelson interferometer, respectively. The optical arrangement has been set up and compared with the well-known 4f optical system. It is found that the proposed optical arrangement is simple to construct, and the brightness distribution is uniform over the whole image. The new setup has been applied to the inspection of a cylindrical steel shell with a rubber plate bonding on its inside surface. The debonding defects have been evaluated using the proposed shearography system at a working distance of 200 mm. By selecting an objective lens with a focal length of 2.8 mm, the viewing angle was enlarged in inspecting a field with 500 mm by 500 mm at one shot.
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http://dx.doi.org/10.1364/AO.417373DOI Listing
February 2021

Magnetism-Responsive Anisotropic Film with Self-Sensing and Multifunctional Shape Manipulation.

ACS Appl Mater Interfaces 2021 Mar 10;13(11):13724-13734. Epub 2021 Mar 10.

Shanghai Institute of Applied Mathematics and Mechanics, School of Mechanics and Engineering Science, Shanghai University, Shanghai 200444, China.

At small scales, shape-programmable magnetic materials and self-sensing conductive materials have an enticing potential for realizing the functionalities that are unattainable by traditional machines. This work reports a facile preparation method of aself-sensing magnetism-responsive anisotropic films (SMAF) in which magnetic materials and conductive materials can be predesigned, oriented, and patterned without requiring an external magnetic field generator or other expensive devices. A variety of shaped magnetoactive films with complex chain-orientation structures that can achieve advanced actuation functions have been developed, such as magnetically driven flowers, windmills, and leaves. It is also verified that the as-prepared samples coated with the sensing layer can distinguish different actuation modes, such as inward bending, outward bending, twisting, and combined deformation, which would be conducive to further exploration and development of directionally responsive applications in the smart actuating system and soft robotics.
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http://dx.doi.org/10.1021/acsami.1c01721DOI Listing
March 2021

Infrapyloric lymph node dissection in right hemicolectomy for colon cancer: Should prophylactic resection be recommended?

J Surg Oncol 2021 May 1;123 Suppl 1:S30-S35. Epub 2021 Mar 1.

Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background And Objectives: Infrapyloric lymph node dissection in right colon cancer patients remains controversial. We aimed to investigate the pattern of infrapyloric lymph node metastasis in right colon cancer patients.

Methods: Clinical and pathological data of 140 colon cancer patients who underwent radical right hemicolectomy and infrapyloric lymph node dissection were retrospectively examined. Patient characteristics, intraoperative conditions, postoperative recovery information, postoperative pathological findings, and follow-up data were examined.

Results: About 19, 44, 73, and 4 patients had tumors located in the cecum, ascending colon, hepatic flexure, and right side of the transverse colon, respectively. The median number of harvested lymph nodes and that of positive lymph nodes were 24 (16-30) and 1 (0-7.75), respectively. The lymph node metastasis rate was 41.43% (58/140). The corresponding values for infrapyloric lymph nodes were 3 (1-4), 0 (0-0), and 0.71% (1/140), respectively. The median follow-up duration was 19 (0-65) months in 131 (93.6%) patients. The 5-year overall and disease-free survival rates were 86.3% and 73.5%, respectively.

Conclusion: Given the low rate of infrapyloric lymph node metastasis in right colon cancer, lymph node dissection is recommended in patients with locally advanced colon cancer at the hepatic flexure and those with suspected infrapyloric lymph node metastasis.
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http://dx.doi.org/10.1002/jso.26388DOI Listing
May 2021

Long non-coding RNA Lnc-LALC facilitates colorectal cancer liver metastasis via epigenetically silencing LZTS1.

Cell Death Dis 2021 02 26;12(2):224. Epub 2021 Feb 26.

The First School of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu, PR China.

Colorectal cancer (CRC) is one of the most common cancers around the world and endangers human health seriously. Liver metastasis is an important factor affecting the long-term prognosis of CRC and the specific mechanism of CRLM (colorectal cancer with liver metastasis) is not fully understood. LZTS1 has been found dysregulated in many cancers, especially in CRC. Theories suggested that hypermethylation of the promoter regions of LZTS1 was responsible for LZTS1 abnormal expression in multiple malignant tumors. Although the role of LZTS1 in CRC cell proliferation has been reported, its role in CRLM remains unclear. Numerous studies reported Long non-coding RNA (lncRNA) could regulate the gene expression level by regulating gene methylation status in many tumors. However, whether there were lncRNAs could change the methylation status of LZTS1 or not in CRLM was unknown. In this study, we aimed to investigate whether there are lncRNAs can regulate the expression of LZTS1 through affecting DNA methylation in CRLM. We found that upregulated Lnc-LALC in CRC was negatively correlated with LZTS1 expression, and Lnc-LALC could regulate LZTS1 expression in both mRNA and protein level in our study. Functionally, Lnc-LALC enhanced the CRC cells metastasis ability in vitro and vivo through inhibiting the expression of LZTS1. Furthermore, the precise mechanisms exploration showed that lnc-LALC could recruit DNA methyltransferases (DNMTs) to the LZTS1 promoter by combining with Enhancer of zeste homolog 2(EZH2) and then altered the expression of LZTS1 via DNMTs-mediated DNA methylation. Collectively, our data demonstrated the important role of Lnc-LALC/ LZTS1 axis in CRLM development.
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http://dx.doi.org/10.1038/s41419-021-03461-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910484PMC
February 2021

Functional Disconnection of the Angular Gyrus Related to Cognitive Impairment in Patients With Type 2 Diabetes Mellitus.

Front Hum Neurosci 2021 3;15:621080. Epub 2021 Feb 3.

Department of MRI, Shaanxi Provincial People's Hospital, Xi'an, China.

Type 2 diabetes mellitus (T2DM) is related to a variety of cognitive impairments that may even progress to dementia. Studies have found the angular gyrus (AG) is a cross-modal integration hub that is involved in a variety of cognitive processes. However, few studies have focused on the patterns of resting-state functional connections (rsFCs) of the AG in patients with T2DM. This study explored the functional connection (FC) between the AG and the whole brain and the relationship between the FC and clinical/cognitive variables in patients with T2DM. 44 patients with T2DM and 43 sex-, age-, and education-matched healthy controls underwent resting-state fMRI and received neuropsychological assessments. Compared with the control group, the T2DM group showed abnormal rsFCs between the AG and multiple brain regions. The FC between the left AG and the left medial temporal lobe in the T2DM group was positively correlated with scores on the Montreal Cognitive Assessment, after a Bonferroni correction ( = 0.40, = 0.009). Collectively, patients with T2DM have abnormal FCs between the AG and extensive brain regions that may be related to various cognitive processes.
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http://dx.doi.org/10.3389/fnhum.2021.621080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886792PMC
February 2021

Application of 3D printing and framework internal fixation technology for high complex rib fractures.

J Cardiothorac Surg 2021 Feb 15;16(1). Epub 2021 Feb 15.

Department of Thoracic Surgery, the Second Hospital of Hebei Medical University, No. 215, Heping west road, Shijiazhuang, 050000, China.

Objective: To explore the clinical effect of 3D printing combined with framework internal fixation technology on the minimally invasive internal fixation of high complex rib fractures.

Methods: Total 16 patients with high complex rib fractures were included in the study. Before the procedure, the 3D rib model was reconstructed based on the thin-layer chest CT scan. According to the 3D model, the rib locking plate was pre-shaped, and the preoperative planning were made including the direction of the locking plate, the location of each nail hole and the length of the screw. During the operation, the locking plate was inserted from the sternum to the outermost fracture lines of ribs with screws at both ends. In addition, the locking plate was used as the frame to sequentially reduce the middle fracture segment and fix with screws or steel wires. Chest x-rays or chest CT scans after surgery were used to assess the ribs recovery. All patients were routinely given non-steroidal anti-inflammatory drugs (NSAIDS) for analgesia, and the pain level was evaluated using numerical rating scale (NRS).

Results: The preoperative planning according to the 3D printed rib model was accurate. The reduction and fixation of each fracture segment were successfully completed through the framework internal fixation technology. No cases of surgical death, and postoperative chest pain was significantly alleviated. Five to 10 months follow up demonstrated neither loosening of screws, nor displacement of fixtures among patients. The lungs of each patients were clear and in good shape.

Conclusion: The application of 3D printing combined with framework internal fixation technology to the high complex rib fractures is beneficial for restoring the inherent shape of the thoracic cage, which can realize the accurate and individualized treatment as well as reduces the operation difficulty.
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http://dx.doi.org/10.1186/s13019-020-01377-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883420PMC
February 2021

A new species of glass sponge (Hexactinellida: Sceptrulophora: Uncinateridae) from the Weijia Seamount in the northwestern Pacific Ocean.

Zootaxa 2020 Nov 13;4878(2):zootaxa.4878.2.6. Epub 2020 Nov 13.

Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, P. R. China Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, P. R. China.

In this article we report on a hexactinellid sponge new to science, Tretopleura weijicus sp. nov., which was collected from the Weijia Seamount in the northwestern Pacific Ocean at a depth of 1995 m. Its blade-like and branching body form, the primary choanosomal framework consisting of multiaxial longitudinal strands without synapticula, and the presence of sceptrules suggest placement within the family Uncinateridae Reiswig, 2002, which is also supported by molecular phylogenetic evidence from COI and 16S sequences. The absence of swollen dermal spurs or regular pentactins and the presence of two types of discohexasters characterize it as a new species. More specimens should be collected to revise the generic characters.
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http://dx.doi.org/10.11646/zootaxa.4878.2.6DOI Listing
November 2020

Application of a New Type of Natural Calcined Bone Repair Material Combined with Concentrated Growth Factors in Bone Regeneration in Rabbit Critical-Sized Calvarial Defect.

Biomed Res Int 2020 24;2020:8810747. Epub 2020 Nov 24.

Department of Stomatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

Purpose: This study is aimed at investigating bone regeneration in critical-sized defects in rabbit calvarium using a novel nano- (n-) hydroxyapatite hybrid scaffold with concentrated growth factors (CGFs).

Methods: Twenty-four male adult rabbits were chosen to establish a critical-sized bone defect model and randomly divided into two groups. Two defects of 15 mm diameter each were created in the parietal bone of each animal. Group A had n-hydroxyapatite hybrid scaffold placed in the experimental defect on the right, and the left defect was unfilled as blank. Group B had hydroxyapatite hybrid scaffold mixed with CGF placed in the right defect and CGF on the left. Six animals in each group were sacrificed after 6 and 12 weeks. Cone-beam computed tomography system scanning and hematoxylin and eosin (HE) staining were used to detect osteogenesis within the defects.

Results: The treatment with n-hydroxyapatite hybrid scaffold along with CGF resulted in a significantly higher amount of new bone at 6 and 12 weeks compared to the treatment with CGF alone and the controls. No apparent inflammation and foreign body reaction were observed through HE staining.

Conclusions: The new synthesized n-hydroxyapatite hybrid scaffold and CGF can be applied for bone defect regeneration to promote the process to a certain extent.
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http://dx.doi.org/10.1155/2020/8810747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707950PMC
May 2021

Circulating non-coding RNA cluster predicted the tumorigenesis and development of colorectal carcinoma.

Aging (Albany NY) 2020 11 21;12(22):23047-23066. Epub 2020 Nov 21.

Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China.

Carcinoembryonic antigen (CEA) is the most significant plasma biomarker in colorectal cancer (CRC), which is mainly used to diagnose and monitor the recurrence of CRC. However, due to the low sensitivity of CEA, it is more recommended for postoperative surveillance rather than early diagnosis. It is necessary to find efficient biomarkers for CRC. In this study, the expression of plasma non-coding RNAs was confirmed in three independent cohorts with total 1201 participants. First, 12 non-coding RNAs were screened from 9 plasma samples by using microarray. The expression of selected non-coding RNAs was further validated by multiphase detection and risk score analysis. We found that miR-20b-5p, miR-329-3p, miR-374b-5p, miR-503-5p, XLOC_001120 and ENSG00000243766.2 were significantly elevated in CRC plasma, and the AUC in training and validation set was 0.996 and 0.954, respectively. Moreover, miR-20b-5p, miR-329-3p and miR-503-5p were found elevated in plasma from larger tumors (5 cm as the cutoff) in CRC patients, and the merged AUC in training and validation set was 0.896 and 0.881. In conclusion, a panel of 6 non-coding RNAs showed their important clinical value for the early diagnosis of CRC. Among, miR-20b-5p, miR-329-3p and miR-503-5p might be the potential markers for evaluating larger tumor size of CRC.
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http://dx.doi.org/10.18632/aging.104055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746361PMC
November 2020

Core-shell [email protected] organic polymer nanocomposites: a multifunctional nanocatalytic agent for primary and metastatic tumor treatment.

J Mater Chem B 2020 12;8(48):11021-11032

Key Laboratory of Functional Nanomaterials and Technology in Universities of Shandong, Linyi University, Linyi 276000, Shandong, P. R. China. and School of Chemistry & Chemical Engineering, Linyi University, Linyi 276000, Shandong, P. R. China.

Metastasis and spread are currently the main factors leading to high mortality of cancer, so developing a synergetic antitumor strategy with high specificity and hypotoxicity is in urgent demand. Based on the design concept of "nanocatalytic medicine", multifunctional nanotherapeutic agent [email protected] nanocomposites (FPCF NCs) are developed for cancer treatment. Specifically, in the tumor microenvironment (TME), FePt could catalyze intracellular over-expressed H2O2 to generate highly active hydroxyl radicals (˙OH), which could not only induce the apoptosis of tumor cells, but also activate the "ferroptosis" pathway resulting in the lipid peroxide accumulation and ferroptotic cell death. Moreover, owing to the excellent photothermal effect, the FPCF NCs could effectively ablate primary tumors under near-infrared (NIR) laser irradiation and produce numerous tumor-associated antigens in situ. With the assistance of a checkpoint blockade inhibitor, anti-CTLA4 antibody, the body's specific immune response would be initiated to inhibit the growth of metastatic tumors. In particular, such synergistic therapeutics could produce an effective immunological memory effect, which could prevent tumor metastasis and recurrence again. In summary, the FPCF NC is an effective multifunctional antitumor therapeutic agent for nanocatalytic/photothermal/checkpoint blockade combination therapy, which exhibits great potential in nanocatalytic anticancer therapeutic applications.
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http://dx.doi.org/10.1039/d0tb01981jDOI Listing
December 2020

Determining the scale of coal mining in an ecologically fragile mining area under the constraint of water resources carrying capacity.

J Environ Manage 2021 Feb 10;279:111621. Epub 2020 Nov 10.

State Key Laboratory of Coal Resources and Safe Mining (CUMT), School of Mines, China University of Mining & Technology, Xuzhou, China.

A reasonable mining scale is very important for the development of mining areas. In view of the lack of water resources in arid and semi-arid areas, this paper studies the scale of coal mining in arid and semi-arid areas under the constraint of the water resources carrying capacity (WRCC) with the aim of realizing the conservation mining of ecological environment. From the perspectives of market demand side, production side and the constraint side, a "trinity" decision model was constructed to investigate the main factors influencing the scale of coal mining. By introducing the optimal control theory with profit taken as objective function, the coal price and coal reserves were regarded as boundary conditions, and WRCC was set as constraint condition. Based on H-J-B equation algorithm, the decision-making equation for mining scale under the constraints of market demand and WRCC was obtained. Through comparing the mining scales under the two constraints, the mode of "water-based mining scale" was formulated, which is conductive for realizing the balance between coal mining and ecological environment development.
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http://dx.doi.org/10.1016/j.jenvman.2020.111621DOI Listing
February 2021

The Diagnostic and Prognostic Value of Serum lncRNA NEAT1 in Colorectal Cancer.

Cancer Manag Res 2020 2;12:10985-10992. Epub 2020 Nov 2.

Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, People's Republic of China.

Background: Colorectal cancer (CRC) is one of the most frequent types of gastrointestinal tract cancers. Abnormal expression of long non-coding RNAs (lncRNAs) has been shown to be closely associated with the progression of CRC. The aim of the current study was to determine the diagnostic and prognostic value of lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in CRC.

Methods: The expression levels and diagnostic value of serum NEAT1 were evaluated in the training and validation cohorts. Then, the prognosis value of serum NEAT1 in CRC was further explored.

Results: The expression levels of serum NEAT1 were significantly higher in CRC, especially in metastatic CRC, than in colorectal adenoma and healthy controls. In addition, serum NEAT1 could well differentiate metastatic CRC from non-metastatic CRC and CRC from colorectal adenoma or healthy controls. Moreover, the metastatic CRC cells secreted more NEAT1 than the control cells. Upregulation of serum NEAT1 was significantly associated with poor clinical outcome of CRC, and serum NEAT1 was identified as an independent prognostic factor for CRC.

Conclusion: Serum NEAT1 might be a promising diagnostic and prognostic biomarker for CRC.
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http://dx.doi.org/10.2147/CMAR.S269978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646461PMC
November 2020

Isolation and characterization of Uropathogenic Escherichia coli (UPEC) from red panda (Ailurus fulgens).

BMC Vet Res 2020 Oct 27;16(1):404. Epub 2020 Oct 27.

Chengdu Research Base of Giant Panda Breeding, Chengdu, 610081, Sichuan, China.

Background: Disease prevention and control is a significant part in the ex-situ conservation of the endangered red panda (Ailurus fulgens), being bacterial infection is one of the most important health threats to the captive population. To date, studies about the infection caused by Escherichia coli in the red panda are scarce. This study was conducted to determine the cause of death of a captive red panda through clinical symptoms, complete blood count, biochemical analysis, pathological diagnosis and bacterial whole genome sequencing.

Case Presentation: The following report describes a case of a 1.5 year old captive red panda (Ailurus fulgens) that was found lethargic and anorectic. She was moved to the quarantine area for daily treatment with 50 mg of Cefpodoxime Proxetil. During the three-day treatment, she did not eat or defecate, and then died. Clinical hematology revealed the values of neutrophils, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and blood urea nitrogen (BUN) were significantly higher. Histological analysis demonstrated major pathological damage in the kidneys, liver and lungs, characterized by hyperemia, parenchymal cell degeneration and necrosis and inflammatory cell infiltration which were predominantly neutrophilic. A bacterial strain confirmed as Escherichia coli was isolated post mortem. Whole genome sequencing of the E. coli showed the complete genome size was 4.99 Mbp. PapA, PapC, OmpA, OmpU and other virulence factors which specific to Uropathogenic Escherichia coli (UPEC) were found in the isolate. Among the virulence factors, P pili, type I pili and related factors of the iron uptake system were associated with nephrotoxicity.

Conclusion: The red panda died of bacterial infection caused by an uropathogenic strain of Escherichia coli. The pathogenic mechanisms of the strain are closely related to the expression of specific virulence genes.
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http://dx.doi.org/10.1186/s12917-020-02624-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590469PMC
October 2020

Median Effective Dose of Lidocaine for the Prevention of Pain Caused by the Injection of Propofol Formulated with Medium- and Long-Chain Triglycerides Based on Lean Body Weight.

Pain Med 2021 06;22(6):1246-1252

Department of Anesthesiology, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.

Objective: To determine the median effective dose (ED50) of prophylactic intravenous lidocaine for the prevention of propofol medium-chain triglyceride/long-chain triglyceride (MCT/LCT) emulsion injection pain.

Design: Prospective trial, Dixon up-and-down sequential method.

Setting: Operating room of a single hospital.

Patients: Thirty patients aged 18-65 years with American Society of Anesthesiologists (ASA) status I or II who were scheduled for elective surgery under general anesthesia (GA) were included.

Interventions: The initial dose of prophylactic lidocaine before propofol MCT/LCT emulsion injection was set at 0.5 mg/kg lean body weight (LBW). The lidocaine dose was adjusted according to the degree of patients' injection pain using the Dixon up-and-down sequential method.

Measurements: The ED50 and 95% confidence intervals (CIs) of lidocaine were calculated using the Dixon-Massey formula. Vital signs and adverse effects were recorded. In the postanesthesia care unit (PACU), patients were asked if they recalled feeling any injection pain with visual analog scale (VAS) evaluation.

Results: The ED50 of lidocaine for the prevention of propofol MCT/LCT emulsion injection pain was 0.306 mg/kg LBW (95% CI, 0.262-0.357 mg/kg LBW). No adverse reactions to lidocaine occurred. In the PACU, 90.9% of patients who experienced injection pain recalled this pain (VAS score, 2.8±1.8).

Conclusions: Prophylactic intravenous lidocaine (0.306 mg/kg LBW) effectively prevented propofol MCT/LCT emulsion injection pain in 50% of patients scheduled for elective surgery under GA with no adverse reaction occurring.
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http://dx.doi.org/10.1093/pm/pnaa316DOI Listing
June 2021

Biomechanical Analysis of Grafted and Nongrafted Maxillary Sinus Augmentation in the Atrophic Posterior Maxilla with Three-Dimensional Finite Element Method.

Scanning 2020 2;2020:8419319. Epub 2020 Oct 2.

Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

This study is aimed at determining the optimal sinus augmentation approach considering the poor bone condition in the zone of atrophic posterior maxilla. A series of simplified maxillary segment models varying in residual bone height (RBH) and bone quality were established. A 10 mm standard implant combined with two types of maxillary sinus augmentation methods was applied with the RBH, which was less than 10 mm in the maxilla. The maximal equivalent von Mises (EQV) stress in residual bone was evaluated. Bone quality had an enormous impact on the stress magnitude of supporting bone. Applying sinus augmentation combined with grafts was suitable for stress distribution, and high-stiffness graft performed better than low-stiffness one. For 7 mm and 5 mm atrophic maxilla, nongrafted maxillary sinus augmentation was feasible in D3 bone. Poor bone quality was a negative factor for the implant in the region of atrophic posterior maxilla, which could be improved by grafts. Meanwhile, the choice of maxillary sinus augmentation approaches should be determined by the RBH and quality.
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http://dx.doi.org/10.1155/2020/8419319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556061PMC
October 2020