Publications by authors named "Dongmei Liu"

206 Publications

Transcriptome Sequencing Reveals the Potential Mechanisms of Modified Electroconvulsive Therapy in Schizophrenia.

Psychiatry Investig 2021 Apr 29. Epub 2021 Apr 29.

Department of Psychiatry, Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Affiliated Hospital of Southwest Medical University, Luzhou, China.

Objective: Schizophrenia (SCZ) is one of the most common and severe mental disorders. Modified electroconvulsive therapy (MECT) is the most effective therapy for all kinds of SCZ, and the underlying molecular mechanism remains unclear. This study is aim to detect the molecule mechanism by constructing the transcriptome dataset from SCZ patients treated with MECT and health controls (HCs).

Methods: Transcriptome sequencing was performed on blood samples of 8 SCZ (BECT: before MECT; AECT: after MECT) and 8 HCs, weighted gene co-expression network analysis (WGCNA) was used to cluster the different expression genes, enrichment and protein-protein interaction (PPI) enrichment analysis were used to detect the related pathways.

Results: Three gene modules (black, blue and turquoise) were significantly associated with MECT, enrichment analysis found that the long-term potentiation pathway was associated with MECT. PPI enrichment p-value of black, blue, turquoise module are 0.00127, <1×10-16 and 1.09×10-13, respectively. At the same time, EP300 is a key node in the PPI for genes in black module, which got from the transcriptome sequencing data.

Conclusion: It is suggested that the long-term potentiation pathways were associated with biological mechanism of MECT.
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http://dx.doi.org/10.30773/pi.2020.0410DOI Listing
April 2021

Nomograms for Predicting Axillary Lymph Node Status Reconciled With Preoperative Breast Ultrasound Images.

Front Oncol 2021 7;11:567648. Epub 2021 Apr 7.

College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China.

Introduction: The axillary lymph node (ALN) status of breast cancer patients is an important prognostic indicator. The use of primary breast mass features for the prediction of ALN status is rare. Two nomograms based on preoperative ultrasound (US) images of breast tumors and ALNs were developed for the prediction of ALN status.

Methods: A total of 743 breast cancer cases collected from 2016 to 2019 at the Second Affiliated Hospital of Harbin Medical University were randomly divided into a training set (n = 523) and a test set (n = 220). A primary tumor feature model (PTFM) and ALN feature model (ALNFM) were separately generated based on tumor features alone, and a combination of features was used for the prediction of ALN status. Logistic regression analysis was used to construct the nomograms. A receiver operating characteristic curve was plotted to obtain the area under the curve (AUC) to evaluate accuracy, and bias-corrected AUC values and calibration curves were obtained by bootstrap resampling for internal and external verification. Decision curve analysis was applied to assess the clinical utility of the models.

Results: The AUCs of the PTFM were 0.69 and 0.67 for the training and test sets, respectively, and the bias-corrected AUCs of the PTFM were 0.67 and 0.67, respectively. Moreover, the AUCs of the ALNFM were 0.86 and 0.84, respectively, and the bias-corrected AUCs were 0.85 and 0.81, respectively. Compared with the PTFM, the ALNFM showed significantly improved prediction accuracy ( < 0.001). Both the calibration and decision curves of the ALNFM nomogram indicated greater accuracy and clinical practicality. When the US tumor size was ≤21.5 mm, the Spe was 0.96 and 0.92 in the training and test sets, respectively. When the US tumor size was greater than 21.5 mm, the Sen was 0.85 in the training set and 0.87 in the test set. Our further research showed that when the US tumor size was larger than 35 mm, the Sen was 0.90 in the training set and 0.93 in the test set.

Conclusion: The ALNFM could effectively predict ALN status based on US images especially for different US tumor size.
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http://dx.doi.org/10.3389/fonc.2021.567648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058421PMC
April 2021

Precise engineering of hybrid molecules-loaded macromolecular nanoparticles shows and antitumor efficacy toward the treatment of nasopharyngeal cancer cells.

Drug Deliv 2021 Dec;28(1):776-786

Department of Radiation Oncology, Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.

Cancers continue to be the second leading cause of death worldwide. Despite the development and improvement of surgery, chemotherapy, and radiotherapy in cancer management, effective tumor ablation strategies are still in need due to high cancer patient mortality. Hence, we have established a new approach to achieve treatment-actuated modifications in a tumor microenvironment by using synergistic activity between two potential anticancer drugs. Dual drug delivery of gemcitabine (GEM) and cisplatin (PT) exhibits a great anticancer potential, as GEM enhances the effect of PT treatment of human cells by providing stability of the microenvironment. However, encapsulation of GEM and PT fanatical by methoxypoly(ethylene glycol)-block-poly(D, L-lactic acid) (PEG-PLA in termed as NPs) is incompetent owing to unsuitability between the binary Free GEM and PT core and the macromolecular system. Now, we display that PT can be prepared by hydrophobic coating of the dual drug centers with dioleoylphosphatidic acid (DOPA). The DOPA-covered PT can be co-encapsulated in PLGA NPs alongside GEM to stimulate excellent anticancer property. The occurrence of the PT suggestively enhanced the encapsulations of GEM into PLGA NPs (GEM-PT NPs). Further, the morphology of GEM NPs, PT NPs, and GEM-PT NPs and nanoparticle size was examined by transmission microscopy (TEM), respectively. Furthermore GEM-PT NPs induced significant apoptosis in human nasopharyngeal carcinoma CNE2 and SUNE1 cancer cells by . The morphological observation and apoptosis were confirmed by the various biochemical assays (AO-EB, nuclear staining, and annexin V-FITC). In a xenograft model of nasopharyngeal cancer, this nanotherapy shows a durable inhibition of tumor progression upon the administration of a tolerable dose. Our results suggest that a macromolecular hydrophobic and highly toxic drug can be rationally converted into a pharmacologically efficient and self-deliverable of nanotherapy.
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http://dx.doi.org/10.1080/10717544.2021.1902022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079022PMC
December 2021

Irisin enhances osteogenic differentiation of mouse MC3T3-E1 cells via upregulating osteogenic genes.

Exp Ther Med 2021 Jun 31;21(6):580. Epub 2021 Mar 31.

Department of Orthopaedics, Peking University People's Hospital, Beijing 10000, P.R. China.

Osteoporosis affects millions of individuals and remains a clinical challenge in terms of prevention and treatment. The present study aimed to investigate the effect of irisin on osteogenic differentiation by exposing MC3T3-E1 cells to different concentrations of irisin. Treated cells were assayed for osteoblast proliferation and osteogenic differentiation by measuring alkaline phosphatase (ALP) activity, calcium deposition, formation of mineralized nodules and the expression of osteogenic genes using reverse transcription-quantitative PCR. The proliferation of MC3T3-E1 cells was unaffected by irisin at the concentrations tested of up to 100 ng/ml (P>0.05). ALP activity and mineralized nodule formation were significantly enhanced by irisin in a dose- and time-dependent manner, indicating that irisin promotes osteoblast differentiation of MC3T3-E1 cells. The expression of osteogenic genes, including ALP, collagen I, runt-related transcription factor 2, osterix, osteopontin, osteocalcin, osteoprotegerin and estrogen receptor α, increased significantly after irisin treatment. The present study demonstrated that irisin promoted the osteogenic differentiation of MC3T3-E1 cells, possibly by upregulating the expression of osteogenic genes and markers. Therefore, irisin may be worthy of further investigation as a potential therapeutic agent for osteoporosis.
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http://dx.doi.org/10.3892/etm.2021.10012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027760PMC
June 2021

3D Porous Ru-Doped NiCo-MOF Hollow Nanospheres for Boosting Oxygen Evolution Reaction Electrocatalysis.

Inorg Chem 2021 Apr 2;60(8):5882-5889. Epub 2021 Apr 2.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Renai Road, Suzhou 215123, P. R. China.

Developing high-performance and cost-efficient catalysts toward oxygen evolution reaction (OER) is an important but daunting task due to the sluggish kinetics hindered by the four-electron transfer process. Herein, an advanced class of ultralow Ru-doped NiCo-MOF hollow porous nanospheres (denoted as Ru@NiCo-MOF HPNs) has been reported in this work. Benefiting from the high porosity and large surface area of the metal-organic frameworks (MOFs) and optimized electronic properties by Ru doping, the as-prepared Ru@NiCo-MOF HPNs exhibit superior performance for water oxidation with the overpotential of only 284 mV to reach a current density of 10 mA·cm in alkaline electrolyte, as well as a small Tafel slope of 78.8 mV·dec, outperforming the NiCo-MOF HPNs (358 mV) and commercial RuO catalyst (326 mV). The incorporation of Ru in NiCo-MOF HPNs enables a stable OER activity for at least 39 h. Moreover, we have probed the interaction between the content of Ru and OER performance, impressively, Ru@NiCo-MOF HPNs with 13.5 atom % Ru doping (denoted as Ru@NiCo-MOF-4) exhibited the highest OER activity with the excellent mass activity of 310 mA·mg at an overpotential of 284 mV. Besides, a two-electrode cell with Ru@NiCo-MOF-4 as the anode and commercial Pt/C catalyst as the cathode also demonstrated outstanding electrocatalytic overall water splitting performance with a cell potential of merely 1.57 V to deliver a current density of 10 mA·cm.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00295DOI Listing
April 2021

First report of chili pepper fruit rot caused by Fusarium incarnatum in China.

Plant Dis 2021 Mar 23. Epub 2021 Mar 23.

Shangqiu Normal University, Shangqiu 476000, Henan, P.R. ChinaShangqiu, China, 476000;

Pepper (Capsicum annuum L.), with annual production over 1 million tons, is ranked the first vegetable crop in Hainan Province, China. In December 2018, fruit rot of chili pepper , with yield loss of up to 15%, was found in 10 fields (12 hm2) in Yacheng (18°N, 109°E), Hainan Province, China. Water-soaked and soft lesions developed on fruit, with white to light gray fungal mycelium present inside. The diseased fruit turned soft and decayed at the later stages. Diseased tissue was cut into 12 pieces of 0.5×0.5 cm, surface-disinfected with 2% sodium hypochlorite for 2 min, followed by 70% ethanol for 30 s, rinsed with sterile distilled water five times, and plated onto potato dextrose agar (PDA). After growing on PDA for 2 to 3 days at 28°C in an incubator without light, 10 pure culture isolates were obtained. All isolates had abundant dense white aerial mycelia that became beige with age. The macroconidia were slightly curved with four to seven septa, 29.51 to 42.15 × 4.29 to 6.22 μm. Spindle-shaped mesoconidia with three to four septa were abundantly produced, 20.34 to 24.54 × 4.58 to to 11.70 × 2.35 to 3.20 μm. Chlamydospores were absent. Based on the morphological characteristics, the fungus was tentatively identified as Fusarium incarnatum (Leslie and Summerell 2006). An isolate SQHP-01 was chosen for molecular identification and pathogenicity test. Two DNA fragments of the isolate, the internal transcribed spacer (ITS) and translation elongation factor genes (EF-1α) were amplified for sequencing. BLAST analysis showed that sequences of ITS (GenBank acc. no. MN317371) and EF-1α (acc. No. MN928788) had 99.61 to 100% identity with those of known F. incarnatum (MN480497 and KF993969). Phylogenetic analysis was conducted using neighbor-joining algorithm based on ITS and EF-1a genes separately, and the isolate was well clustered with F. incarnatum both with 100% bootstrap support. Pathogenicity test of the isolate were carried out twice on five healthy chili pepper fruit. After surface-disinfection, fruit were wounded at three different points and 20 μl of conidial suspension (106 conidia/ml) were deposited on each wound. Unwounded inoculation was conducted by spreading 100 μl of the suspension on each fruit surface including the pedicel and calyx. The fruit spread with sterile distilled water represented the negative control. All fruit treatments were placed on the moist sterile cotton in moist chambers at 25°C with 16 h light and 8 h darkness. After 4 to 6 days, water-soaked necrotic lesions appeared on the wounded fruit, the symptoms identical to those observed in the field. Water-soaked necrotic lesions developed on the pedicel and calyx of unwounded fruit. No symptoms were observed on the control fruit. The morphology and sequences of re-isolated fungal isolates from the tested peppers were the same as the original isolate. To our knowledge, this is the first report of F. incarnatum (synonym of F. semitectum) causing fruit rot on chili pepper in China. F. incarnatum has been reported to cause root rot of greenhouse pepper in China (Li et al. 2018), fruit rot of bell pepper in Trinidad (Ramdial et al. 2016) and Pakistan (Tariq et al. 2018). Effective control strategies need to be developed to prevent the economic losses caused by the disease in chili pepper.
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http://dx.doi.org/10.1094/PDIS-09-19-1996-PDNDOI Listing
March 2021

Generation of a sub-diffracted Bessel beam via diffraction interference in a combined amplitude structure.

Opt Express 2021 Jan;29(2):597-603

We have theoretically investigated the use of a simple combined amplitude structure to produce a sub-diffracted Bessel beam via diffraction interference. This powerful structure is composed of a spiral slit and radial grating. When a vortex beam illuminates this combined amplitude structure, a subwavelength Bessel beam with a size of 0.39λ and a long working distance of approximately 100 µm is numerically realized. By tailoring the parameters of the spiral slit, we can obtain a longer sub-diffracted Bessel beam. Moreover, the observed Bessel beam has low-energy side-lobes. The peculiar features of our theoretically generated Bessel beam have numerous potential applications, such as in nanoparticles manipulation, super-resolution imaging, and lithography.
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http://dx.doi.org/10.1364/OE.410360DOI Listing
January 2021

3D Taraxacum-like porous Pd nanocages with Bi doping: High-performance non-Pt electrocatalysts for ethanol oxidation reaction.

J Colloid Interface Sci 2021 Jun 9;591:203-210. Epub 2021 Feb 9.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Renai Road, Suzhou 215123, PR China. Electronic address:

Modifying the electronic structure and optimizing the geometric structure can expeditiously tune the electrocatalytic properties of catalysts, resulting in considerably enhanced electrocatalytic performance towards electrocatalytic oxidation of liquid fuels. We herein report a simple synthetic strategy to prepare Bi-doped 3D taraxacum-like Pd nanocages (NCs) composed of porous nanosheets, which possess high surface areas and strong synergistic effects. Notably, a trace of Bi diffuses into the lattice of Pd and increases the electronic effects of the surface of Pd, endowing PdBi-0.5 NCs/C with superior electrocatalytic performance towards ethanol oxidation reaction (EOR). The mass activity and specific activity of PdBi-0.5 NCs/C were 3494.8 mA mg and 10.37 mA cm, being 4.08- and 4.82- fold enhancements as compared with commercial Pd/C, respectively. Moreover, the highly open porous 3D nanocages structure with rich active sites and defects can also facilitate the mass/electron transfer to favor the EOR kinetics.
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http://dx.doi.org/10.1016/j.jcis.2021.02.018DOI Listing
June 2021

The complete chloroplast genome of (Plantaginaceae).

Mitochondrial DNA B Resour 2021 Jan 28;6(1):259-260. Epub 2021 Jan 28.

CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.

is an aquatic perennial herb distributed worldwide. In this research, the complete chloroplast genome of was sequenced and assembled. Its complete genome size was 152,698 bp in length. The typical quadripartite structure was shown, which contained a large single-copy region (82,940 bp), a small single-copy region (18,262 bp), and a pair of inverted repeat regions (25,748 bp). The CG content of this genome was 37.6%. A total of 114 genes have been identified in the genome, including 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. In addition, 18 genes possessed at least one intron. The phylogenetic analysis indicated that was nested in Plantaginaceae with 100% bootstrap value and was a sister to , , , and .
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http://dx.doi.org/10.1080/23802359.2020.1860706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850381PMC
January 2021

Epigallocatechin gallate protects the human lens epithelial cell survival against UVB irradiation through AIF/Endo G signaling pathways in vitro.

Cutan Ocul Toxicol 2021 Jan 25:1-25. Epub 2021 Jan 25.

Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, P. R. China.

Objective: Oxidative stress has been recognized as an important mediator of apoptosis in lens epithelial cells. It also plays an important role in the pathogenesis of cataracts. It is reported that (-)-Epigallocatechin gallate (EGCG), the most abundant component in green tea, exhibits potent antioxidant activity against oxidative stress. This study aimed to investigate the protective effect of EGCG against Ultraviolet B (UVB) induced apoptotic death and the underlying mechanism in human lens epithelial cells (HLECs).

Methods: HLECs were exposed to various concentrations of EGCG under UVB (30 mJ/cm), and cell viability was monitored by the MTT assay. Next, mitochondrial membrane potential (Δψm), reactive oxygen species (ROS) and apoptosis were detected by flow cytometry. Meanwhile, the total antioxigenic capacity (T-AOC) was determined by enzyme standard instrument, and the expression of apoptosis inducing factor (AIF) and endonuclease G (Endo G) was measured by quantitative PCR (Q-PCR) and western blotting, respectively. Moreover, the localization of AIF and Endo G within cells was further detected by confocal optical microscopy.

Results: The results indicated that EGCG could enhance the cell viability and protect against cell apoptosis caused by UVB irradiation in HLECs. EGCG could also decrease the UVB-induced generation of ROS and collapse of Δψm, increase the T-AOC level. In addition, EGCG could also inhibit the UVB-stimulated increase of AIF and Endo G expression at mRNA and protein levels and ameliorate the UVB-induced mitochondria-nuclear translocation of AIF and Endo G.

Conclusions: UVB irradiation could damage HLECs viability, while EGCG exhibits antioxidant effect and inhibits UVB-induced apoptosis in HLECs through AIF/Endo G signaling pathways. Our findings reveal the underlying mechanism of EGCG against UVB-induced oxidative stress in HLECs.
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http://dx.doi.org/10.1080/15569527.2021.1879112DOI Listing
January 2021

A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis.

Int J Mol Sci 2021 Jan 14;22(2). Epub 2021 Jan 14.

Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China.

Glycerol-3-phosphate acyltransferases (GPATs) play an important role in glycerolipid biosynthesis, and are mainly involved in oil production, flower development, and stress response. However, their roles in regulating plant height remain unreported. Here, we report that Arabidopsis GPAT1 is involved in the regulation of plant height. GUS assay and qRT-PCR analysis in Arabidopsis showed that is highly expressed in flowers, siliques, and seeds. A loss of function mutation in was shown to decrease seed yield but increase plant height through enhanced cell length. Transcriptomic and qRT-PCR data revealed that the expression levels of genes related to gibberellin (GA) biosynthesis and signaling, as well as those of cell wall organization and biogenesis, were significantly upregulated. These led to cell length elongation, and thus, an increase in plant height. Together, our data suggest that knockout of impairs glycerolipid metabolism in Arabidopsis, leading to reduced seed yield, but promotes the biosynthesis of GA, which ultimately enhances plant height. This study provides new evidence on the interplay between lipid and hormone metabolism in the regulation of plant height.
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http://dx.doi.org/10.3390/ijms22020785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829857PMC
January 2021

New understanding of novel brominated flame retardants (NBFRs): Neuro(endocrine) toxicity.

Ecotoxicol Environ Saf 2021 Jan 13;208:111570. Epub 2020 Nov 13.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

Traditional brominated flame retardants (BFRs) negatively affect the environment and human health, especially in the sensitive (developing) nervous system. Considering the physicochemical similarities between novel brominated flame retardants (NBFRs) and BFRs, more and more evidence reveals the neurotoxic effects of NBFRs. We reviewed the neuro(endocrine) toxic effects of NBFRs in vivo and in vitro and discussed their action mechanisms based on the available information. The neurotoxic potential of NBFRs has been demonstrated through direct neurotoxicity and disruption of the neuroendocrine system, with adverse effects on neurobehavioral and reproductive development. Mechanistic studies have shown that the impact of NBFRs is related to the complex interaction of neural and endocrine signals. From disrupting the gender differentiation of the brain, altering serum thyroid/sex hormone levels, gene/protein expression, and so on, to interfere with the feedback effect between different levels of the HPG/HPT axis. In this paper, the mechanism of neurotoxic effects of NBFRs is explored from a new perspective-neuro and endocrine interactions. Gaps in the toxicity data of NBFRs in the neuroendocrine system are supplemented and provide a broader dataset for a complete risk assessment.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111570DOI Listing
January 2021

The latency-associated transcript locus of herpes simplex virus 1 is a virulence determinant in human skin.

PLoS Pathog 2020 12 28;16(12):e1009166. Epub 2020 Dec 28.

Departments of Pediatrics and Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, United States of America.

Herpes simplex virus 1 (HSV-1) infects skin and mucosal epithelial cells and then travels along axons to establish latency in the neurones of sensory ganglia. Although viral gene expression is restricted during latency, the latency-associated transcript (LAT) locus encodes many RNAs, including a 2 kb intron known as the hallmark of HSV-1 latency. Here, we studied HSV-1 infection and the role of the LAT locus in human skin xenografts in vivo and in cultured explants. We sequenced the genomes of our stock of HSV-1 strain 17syn+ and seven derived viruses and found nonsynonymous mutations in many viral proteins that had no impact on skin infection. In contrast, deletions in the LAT locus severely impaired HSV-1 replication and lesion formation in skin. However, skin replication was not affected by impaired intron splicing. Moreover, although the LAT locus has been implicated in regulating gene expression in neurones, we observed only small changes in transcript levels that were unrelated to the growth defect in skin, suggesting that its functions in skin may be different from those in neurones. Thus, although the LAT locus was previously thought to be dispensable for lytic infection, we show that it is a determinant of HSV-1 virulence during lytic infection of human skin.
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http://dx.doi.org/10.1371/journal.ppat.1009166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794027PMC
December 2020

Contrast-enhanced ultrasound in uterine artery embolization treatment of cesarean scar pregnancy.

J Int Med Res 2020 Dec;48(12):300060520980217

Department of Ultrasound, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Objective: The current study aimed to investigate the application of contrast-enhanced ultrasound (CEUS) in diagnosis and treatment of cesarean scar pregnancy (CSP).

Methods: A retrospective study was performed in 35 patients with clinically suspected CSP who requested termination of pregnancy and underwent contrast-enhanced ultrasound (CEUS). The patients were classified into two groups on the basis of whether they received uterine artery embolization (UAE). The CEUS characteristics of the two groups were reviewed.

Results: CEUS features of CSP were early enhancement of the cesarean scar and continuous infusion of contrast agent between the gestational sac and cesarean scar. Myometrial thickness in the cesarean scar was thinner in the UAE group than in the non-UAE group by CEUS and transvaginal ultrasound. Myometrial thickness measured by CEUS was thinner than that measured by transvaginal ultrasound in both groups. The parameters of the time-intensity curve in the UAE group were characterized by a faster arrival time, shorter time to peak, higher peak intensity, and greater enhancement rate compared with the non-UAE group.

Conclusions: CEUS may be a novel supplementary method to diagnose and assess CSP, and to help evaluate whether UAE is required.
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http://dx.doi.org/10.1177/0300060520980217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7768854PMC
December 2020

Oxygen vacancy-mediated sandwich-structural TiO /ultrathin g-CN/TiO direct Z-scheme heterojunction visible-light-driven photocatalyst for efficient removal of high toxic tetracycline antibiotics.

J Hazard Mater 2021 Apr 31;408:124432. Epub 2020 Oct 31.

Department of Environmental Science, School of Chemistry and Materials Science, Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin 150080, PR China. Electronic address:

A surface defect sandwich-structural TiO/ultrathin g-CN/TiO direct Z-scheme heterojunction photocatalyst is successfully constructed. The results manifest the existence of oxygen vacancies, sandwich structure and direct Z-scheme heterojunction. Noticeably, TiO/ultrathin g-CN/TiO efficiently eliminates high toxic tetracycline hydrochloride by means of·O, h and·OH, whose removal rate is 87.7% during 90 min and the pseudo-first-order rate constant reaches up to 31.7 min × 10. The extraordinary performance can be attributed to the special 3D structure, Z-scheme heterojunction expediting charge transfer and promoting the generation of active species, meanwhile the oxygen vacancies enhancing the spatial separation of photo-induced carriers. Moreover, various environmental factors are systematically explored by statistics. SO, NH-N and pH exhibit an obvious impact on removal rate. Meanwhile, TiO/ultrathin g-CN/TiO could also effectually remove tetracycline hydrochloride from complex actual-wastewater and exhibit high stability. Besides, the photocatalytic mechanism and degradation path of tetracycline hydrochloride are also elucidated.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124432DOI Listing
April 2021

Correlation of Single Nucleotide Gene Polymorphisms and Gastric Cancer Based on Magnetic Nanoparticles.

J Nanosci Nanotechnol 2021 Feb;21(2):928-934

Clinical Experimental Research Centre, Jinan City Central Hospital Affiliated to Shandong University, Jinan City, 250013, Shandong Province, China.

Gastric cancer (GC) is a serious threat to the health and lives of people around the world. In China, the incidence and mortality of gastric cancer are much higher than the world average, coupled with its low early diagnosis rate, low survival rate, poor prognosis, and complex etiology, especially the serious lack of effective early warning methods, which has become the main constraint on the diagnosis and treatment of gastric cancer factor. Therefore, finding reliable, effective, and specific markers that can be applied to early warning and diagnosis of gastric cancer has been a hot issue in gastric cancer research. Magnetic nanoparticles are an ideal molecular carrier for gene separation because they have many advantages such as easy operation, fast, high efficiency, and non-destructive non-recognition biological entities. Changes in gene levels can detect the development of early diagnosis and treatment of prognosis in patients with gastric cancer by affecting susceptibility, clinical phenotype, and drug response. PcG protein can modify chromatin and affect tumorigenesis. The experimental results show that the introduction of magnetic nanoparticles can improve the sensing signal, detection sensitivity and gene differentiation. Combined with the latest magnetic nanoparticle technology to analyze the relationship between SNPs of some genes in the pathways involved in gastric cancer treatment and DNA specificity, screening and identifying specific SNP markers are helpful to the mechanism of gastric cancer development. Understand to achieve the purpose of individualized treatment. By introducing the RAS-BRAF gene on the surface of magnetic nanoparticles, the surface of the magnetic particles was biologically functionalized and used for the separation and detection of proteins and pathogens, respectively. The results show that the system has excellent detection sensitivity and separation selectivity. At present, the research results of susceptible genes screened by coding gene association studies are inconsistent. In this study, PLCE1 gene was found to be used as a DNA gene identification method through high expression of cells to analyze that polymorphisms are closely related to the incidence of gastric cancer. In addition, the study suggests that PLCE1 gene may be a susceptible gene for tumor cells. The signaling pathways involved in the regulation play an important role in tumorigenesis, development, migration, and apoptosis, and are closely related to disease prognosis. Therefore, at the gene level More analysis of the role of these genes in gastric cancer is needed.
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http://dx.doi.org/10.1166/jnn.2021.18628DOI Listing
February 2021

Prognostic factors in IgG4-related disease: a long-term monocentric Chinese cohort study.

Clin Rheumatol 2021 Jun 5;40(6):2293-2300. Epub 2020 Nov 5.

Department of Rheumatology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.

Objectives: Patients with IgG4-related disease (IgG4-RD) suffer high relapse rates during long-term treatment, but factors that predict relapse outcomes are not well established. In the present study, we aimed to identify predictive factors for treatment resistance and disease relapse in a Chinese IgG4-RD cohort.

Methods: This study enrolled 102 patients newly diagnosed with IgG4-RD. Disease prognosis was determined by evaluating disease activity and dosage of glucocorticoids. Predictive factors for refractory and relapsed disease were identified by univariate analysis and Cox regression.

Results: Among the 102 patients, 78 cases received medical treatment with regular follow-up (21 [6-111] months). During the follow-up period, 55 (70.5%) patients sustained clinical remission, and 23 (29.5%) patients suffered refractory or relapsed disease. The relapse rate of the patients with IgG4-RD was significantly higher among patients who stopped taking medicine than among those who continued treatment with glucocorticoids (GC) + immunosuppressor (IM). Serum TNF-α ≥ 13 pg/mL, sIL-2R ≥ 1010 U/mL, total cholesterol < 3.55 mmol/L, low-density lipoprotein < 2.0 mmol/L, IgG ≥ 20.2 g/L, and drug withdrawal were predictive factors for refractory and relapsed IgG4-RD. Multivariate Cox regression revealed that both sIL-2R and TNF-α were independent risk factors for refractory and relapsed disease. The combination of GC and IM treatment was an independent protective factor against refractory and relapsed IgG4-RD.

Conclusions: High serum levels of sIL-2R and TNF-α may be informative risk factors for refractory and relapsed IgG4-RD. Our data suggest that a combination treatment of GC along with IM may be protective against refractory and relapsed IgG4-RD. Key Points • High sIL-2R and TNF-α levels are informative risk factors for refractory and relapsed IgG4-related disease. • Combination treatment of GC with IM protects against refractory and relapsed IgG4-related disease.
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http://dx.doi.org/10.1007/s10067-020-05484-8DOI Listing
June 2021

Flexible tuning of nonlinear non-diffracting array beams using wavelengths and angles.

Opt Lett 2020 Nov;45(21):6106-6109

We present a simple method to enable flexible tuning of non-diffracting beams in a two-dimensional nonlinear photonic crystal, based on the interference of two or more non-collinear second-harmonic beams. By manipulating the wavelengths of the beams and the angle of incidence of the fundamental wave, the arbitrary period and propagation length, as well as the wavelength of the generated nonlinear non-diffracting array beams, can be tuned flexibly. These light beams can trap and manipulate multiple particles, create new forms of optical imaging systems, and act within nonlinear devices to bring novel functionalities to integrated optics.
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November 2020

gcType: a high-quality type strain genome database for microbial phylogenetic and functional research.

Nucleic Acids Res 2021 01;49(D1):D694-D705

Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Taxonomic and functional research of microorganisms has increasingly relied upon genome-based data and methods. As the depository of the Global Catalogue of Microorganisms (GCM) 10K prokaryotic type strain sequencing project, Global Catalogue of Type Strain (gcType) has published 1049 type strain genomes sequenced by the GCM 10K project which are preserved in global culture collections with a valid published status. Additionally, the information provided through gcType includes >12 000 publicly available type strain genome sequences from GenBank incorporated using quality control criteria and standard data annotation pipelines to form a high-quality reference database. This database integrates type strain sequences with their phenotypic information to facilitate phenotypic and genotypic analyses. Multiple formats of cross-genome searches and interactive interfaces have allowed extensive exploration of the database's resources. In this study, we describe web-based data analysis pipelines for genomic analyses and genome-based taxonomy, which could serve as a one-stop platform for the identification of prokaryotic species. The number of type strain genomes that are published will continue to increase as the GCM 10K project increases its collaboration with culture collections worldwide. Data of this project is shared with the International Nucleotide Sequence Database Collaboration. Access to gcType is free at http://gctype.wdcm.org/.
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http://dx.doi.org/10.1093/nar/gkaa957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778895PMC
January 2021

Opa1 Reduces Hypoxia-Induced Cardiomyocyte Death by Improving Mitochondrial Quality Control.

Front Cell Dev Biol 2020 28;8:853. Epub 2020 Aug 28.

Department of Cardiology, Tianjin First Center Hospital, Tianjin, China.

Mitochondrial dysfunction contributes to cardiovascular disorders, especially post-infarction cardiac injury, through incompletely characterized mechanisms. Among the latter, increasing evidence points to alterations in mitochondrial quality control, a range of adaptive responses regulating mitochondrial morphology and function. Optic atrophy 1 (Opa1) is a mitochondrial inner membrane GTPase known to promote mitochondrial fusion. In this study, hypoxia-mediated cardiomyocyte damage was induced to mimic post-infarction cardiac injury . Loss- and gain-of-function assays were then performed to evaluate the impact of Opa1 expression on mitochondrial quality control and cardiomyocyte survival and function. Hypoxic stress reduced cardiomyocyte viability, impaired contractile/relaxation functions, and augmented the synthesis of pro-inflammatory mediators. These effects were exacerbated by Opa1 knockdown, and significantly attenuated by Opa1 overexpression. Mitochondrial quality control was disturbed by hypoxia, as reflected by multiple mitochondrial deficits; i.e., increased fission, defective fusion, impaired mitophagy, decreased biogenesis, increased oxidative stress, and blunted respiration. By contrast, overexpression of Opa1 normalized mitochondrial quality control and sustained cardiomyocyte function. We also found that ERK, AMPK, and YAP signaling can regulate Opa1 expression. These results identify Opa1 as a novel regulator of mitochondrial quality control and highlight a key role for Opa1 in protecting cardiomyocytes against post-infarction cardiac injury.
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http://dx.doi.org/10.3389/fcell.2020.00853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483501PMC
August 2020

Resting Energy Expenditure, Fetal Biometric Parameters by Ultrasound, and Birthweight in Chinese Pregnant Women With Gestational Diabetes Mellitus.

J Ultrasound Med 2021 May 11;40(5):989-996. Epub 2020 Sep 11.

Department of Ultrasound, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing, 100038, China.

Objectives: To explore the relationships between resting energy expenditure (REE) according to indirect calorimetry, fetal biometric parameters by ultrasound, and birthweight in gestational diabetes mellitus (GDM).

Methods: Sixty-five women with GDM and 60 in the control group were enrolled. The REE, birthweight, and fetal biometric parameters according to ultrasound, including biparietal diameter, head circumference, abdominal circumference (AC), and femur length, were measured.

Results: The AC at 29 to 32 weeks and 37 to 40 weeks was larger in the GDM than in the control group (P < 0.01), birthweight was higher in the GDM than in the control group (P < 0.01), and women in the GDM group had higher REE than those in the control group at all stages of pregnancy (P < 0.01). In the control group, all fetal biometric parameters were correlated with birthweight at 37 to 40 weeks (r = 0.418, 0.678, 0.741, and 0.635 for biparietal diameter, head circumference, AC, and femur length, respectively, P < 0.05); however, in the GDM group, only AC was correlated with birthweight at 37 to 40 weeks (r = 0.707; P < 0.05). In the GDM group, REE was correlated with birthweight at all three stages of pregnancy (r = 0.369, 0.381, and 0.446 for 21 to 24, 29 to 32, and 37 to 40 weeks, respectively, P < 0.05), and REE was correlated with AC at 37 to 40 weeks (r = 0.431; P < 0.05).

Conclusions: REE is correlated with birthweight in women with GDM from the middle to the end of pregnancy. REE by indirect calorimetry might be potential index for medical nutrition therapy in GDM.
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http://dx.doi.org/10.1002/jum.15474DOI Listing
May 2021

DNA Encapsidation and Capsid Assembly Are Underexploited Antiviral Targets for the Treatment of Herpesviruses.

Front Microbiol 2020 12;11:1862. Epub 2020 Aug 12.

Department of Biomedical Sciences, Mercer University School of Medicine, Savannah, GA, United States.

Although there are effective nucleoside analogs to treat HSV, VZV, and HCMV disease, herpesvirus infections continue to contribute to significant morbidity and mortality. Acyclovir is the drug of choice for HSV encephalopathy, yet there is an estimated 6-19% mortality rate with half of the survivors experiencing moderate to severe chronic neurological deficits. For VZV, current treatments are inadequate to prevent acute and persistent pain due to zoster. Treatment of HCMV with GCV requires close monitoring particularly in patients with impaired renal function and there are no approved treatments for congenital HCMV infections. New therapeutic options to control cytomegalovirus reactivation in bone marrow and stem cell transplant patients are needed to improve patient outcome. No successful chemotherapeutic options are available for EBV, HHV-6, 7, and 8. Drug resistance is a concern for HCMV, HSV, and VZV since approved drugs share common mechanisms of action. Targeting DNA encapsidation or capsid assembly provide additional options for the development of non-nucleoside, small molecule anti-herpesviral drugs.
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http://dx.doi.org/10.3389/fmicb.2020.01862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434925PMC
August 2020

A Novel Human Skin Tissue Model To Study Varicella-Zoster Virus and Human Cytomegalovirus.

J Virol 2020 10 27;94(22). Epub 2020 Oct 27.

Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York, USA

The herpesviruses varicella-zoster virus (VZV) and human cytomegalovirus (HCMV) are endemic to humans. VZV causes varicella (chicken pox) and herpes zoster (shingles), while HCMV causes serious disease in immunocompromised patients and neonates. More effective, less toxic antivirals are needed, necessitating better models to study these viruses and evaluate antivirals. Previously, VZV and HCMV models used fetal tissue; here, we developed an adult human skin model to study VZV and HCMV in culture and While VZV is known to grow in skin, it was unknown whether skin could support an HCMV infection. We used TB40/E HCMV and POka VZV strains to evaluate virus tropism in skin organ culture (SOC) and skin xenograft mouse models. Adult human skin from reduction mammoplasties was prepared for culture on NetWells or mouse implantation. In SOC, VZV infected the epidermis and HCMV infected the dermis. Specifically, HCMV infected fibroblasts, endothelial cells, and hematopoietic cells, with some infected cells able to transfer infection. VZV and HCMV mouse models were developed by subcutaneous transplantation of skin into SCID/beige or athymic nude mice at 2 independent sites. Viruses were inoculated directly into one xenograft, and widespread infection was observed for VZV and HCMV. Notably, we detected VZV- and HCMV-infected cells in the contralateral, uninoculated xenografts, suggesting dissemination from infected xenografts occurred. For the first time, we showed HCMV successfully grows in adult human skin, as does VZV. Thus, this novel system may provide a much-needed preclinical small-animal model for HCMV and VZV and, potentially, other human-restricted viruses. Varicella-zoster virus and human cytomegalovirus infect a majority of the global population. While they often cause mild disease, serious illness and complications can arise. Unfortunately, there are few effective drugs to treat these viruses, and many are toxic. To complicate this, these viruses are restricted to replication in human cells and tissues, making them difficult to study in traditional animal models. Current models rely heavily on fetal tissues, can be prohibitively expensive, and are often complicated to generate. While fetal tissue models provide helpful insights, it is necessary to study human viruses in human tissue systems to fully understand these viruses and adequately evaluate novel antivirals. Adult human skin is an appropriate model for these viruses because many target cells are present, including basal keratinocytes, fibroblasts, dendritic cells, and lymphocytes. Skin models, in culture and xenografts in immunodeficient mice, have potential for research on viral pathogenesis, tissue tropism, dissemination, and therapy.
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http://dx.doi.org/10.1128/JVI.01082-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592229PMC
October 2020

AgsA oligomer acts as a functional unit.

Biochem Biophys Res Commun 2020 09 28;530(1):22-28. Epub 2020 Jul 28.

Department of Biochemistry and Biophysics, The Health Science Center, Peking University, Beijing, 100191, China; Electron Microscopy Analysis Laboratory, Center of Medical and Health Analysis, Peking University, Beijing, 100191, China. Electronic address:

AgsA (aggregation-suppressing protein) is an ATP-independent molecular chaperone machine belonging to the family of small heat shock proteins (sHSP), and it can prevent the aggregation of non-natural proteins. However, the substrate-binding site of AgsA and the functional unit that captures and binds the substrate remain unknown. In this study, different N-terminal and C-terminal deletion mutants of AgsA were constructed and their effects on AgsA oligomer assembly and chaperone activity were investigated. We found that the IXI motif at the C-terminus and the α-helix at the N-terminus affected the oligomerization and molecular chaperone activity of AgsA. In this work, we obtained a 6.8 Å resolution structure of AgsA using Electron cryo-microscopy (cryo-EM), and found that the functional form of AgsA was an 18-mer with D3 symmetry. Through amino acid mutations, disulfide bonds were introduced into two oligomeric interfaces, namely dimeric interface and non-partner interface. Under oxidation and reduction conditions, the chaperone activity of the disulfide-bonded AgsA did not change significantly, indicating that AgsA would not dissociate to achieve chaperone activity. Therefore, we concluded that the oligomer, especially 18-mer, was the primary functional unit.
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http://dx.doi.org/10.1016/j.bbrc.2020.07.027DOI Listing
September 2020

Monitoring of an Ethanol-Water Exchange Process to Produce Bulk Nanobubbles Based on Dynamic Light Scattering.

Langmuir 2020 Sep 11;36(34):10069-10073. Epub 2020 Aug 11.

Guangdong Provincial Engineering Research Center For Optoelectronic Instrument, SPTE, South China Normal University, Guangzhou 510006, China.

Nanobubbles have been reported to have many novel applications due to their unique physicochemical properties. Ethanol-water exchange is regarded as one of the most convenient methods for producing nanobubbles; however, it is still questioned whether this method can produce bulk nanobubbles or not. In this paper, we present a method to monitor the ethanol-water exchange process based on a setup that combines the equipment of the ethanol-water exchange with an apparatus for dynamic light scattering. In contrast to the previous works where the measurements were performed after the exchanges were completed, our method measures the intensity of the scattered light from the beginning of the process to the end. We found that three different stages of the exchange process can be easily distinguished and that the diameters of the particles produced decrease as the exchange time increases. Furthermore, the measured diameters agree very well with a theoretical model presented very recently for the stability of the bulk nanobubbles in the liquid. Based on these findings, we believe that the products of the ethanol-water exchange are bulk nanobubbles. In addition, since our experimental setup provides the details of the ethanol-water exchange process, it can be used to investigate how to control the parameters of the final nanobubbles, such as their size, concentration, etc., which might promote the potential applications of bulk nanobubbles.
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http://dx.doi.org/10.1021/acs.langmuir.0c01170DOI Listing
September 2020

Circular RNA hsa_circ_0096157 contributes to cisplatin resistance by proliferation, cell cycle progression, and suppressing apoptosis of non-small-cell lung carcinoma cells.

Mol Cell Biochem 2020 Dec 6;475(1-2):63-77. Epub 2020 Aug 6.

Pulmonary and Critical Care Medicine Ward, The First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Qingxiu District, Nanning, 530021, Guangxi, People's Republic of China.

Circular RNAs (circRNAs) play a major role in cancer development and chemotherapy resistance. This study aimed to characterize circRNA profiles associated with Cisplatin (diamminedichloroplatinum, DDP) resistance of non-small-cell lung carcinoma (NSCLC) cells. The half-maximal inhibitory concentration (IC50) of A549 and A549/DDP cells was determined using CCK-8 assay. Further, circRNA profiles and differentially expressed genes in A549 and A549/DDP cells were characterized by deep sequencing and cell proliferation was measured using MTS assay. Cell cycle progression was analyzed using flow cytometry. Apoptosis experiment was performed by TUNEL assay and flow cytometry. Cell migration and invasion were assessed using the Transwell system. Finally, signalling protein levels related to cell cycle progression and migration were measured by western blot. CCK-8 assay showed that A549/DDP cells obtained strong DDP resistance. Further deep sequencing results showed that 689 circRNAs and 87 circRNAs were significantly upregulated and downregulated in A549/DDP cells compared to A549 cells, respectively. Moreover, the circRNA hsa_circ_0096157 with the highest expression level in A549/DPP cells was further analyzed for its potential mechanism of DDP resistance in A549/DDP. With or without DDP treatment, hsa_circ_0096157 knockdown inhibited proliferation, migration, invasion and cell cycle progression but promoted apoptosis of A549/DDP cells. In addition, the western blot results also showed that hsa_circ_0096157 knockdown in A549/DDP cells increased P21 and E-cadherin but decreased CDK4, Cyclin D1, Bcl-2, N-cadherin, and Vimentin protein expression levels, indicating that cell cycle progression might be inhibited by increased P21 protein level to inhibit the expression of CDK4-cyclin D1 complex and decreased Bcl-2 protein level; and migration and invasion were suppressed by the increased E-cadherin and decreased N-cadherin and Vimentin expression levels. In contrast, hsa_circ_0096157 overexpression in A549 cells caused the opposite cellular and molecular alterations. DDP resistance in NSCLC cells was associated with significant circRNA profile alterations. Moreover, increased hsa_circ_0096157 expression contributed to DDP resistance in NSCLC cells by promoting cell proliferation, migration, invasion and cell cycle progression and inhibiting apoptosis.
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http://dx.doi.org/10.1007/s11010-020-03860-1DOI Listing
December 2020

Vps13 is required for the packaging of the ER into autophagosomes during ER-phagy.

Proc Natl Acad Sci U S A 2020 08 20;117(31):18530-18539. Epub 2020 Jul 20.

Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093-0668;

Endoplasmic reticulum (ER) macroautophagy (hereafter called ER-phagy) uses autophagy receptors to selectively degrade ER domains in response to starvation or the accumulation of aggregation-prone proteins. Autophagy receptors package the ER into autophagosomes by binding to the ubiquitin-like yeast protein Atg8 (LC3 in mammals), which is needed for autophagosome formation. In budding yeast, cortical and cytoplasmic ER-phagy requires the autophagy receptor Atg40. While different ER autophagy receptors have been identified, little is known about other components of the ER-phagy machinery. In an effort to identify these components, we screened the genome-wide library of viable yeast deletion mutants for defects in the degradation of cortical ER following treatment with rapamycin, a drug that mimics starvation. Among the mutants we identified was Δ. While yeast has one gene that encodes the phospholipid transporter , humans have four vacuolar protein-sorting (VPS) protein 13 isoforms. Mutations in all four human isoforms have been linked to different neurological disorders, including Parkinson's disease. Our findings have shown that Vps13 acts after Atg40 engages the autophagy machinery. Vps13 resides at contact sites between the ER and several organelles, including late endosomes. In the absence of Vps13, the cortical ER marker Rtn1 accumulated at late endosomes, and a dramatic decrease in ER packaging into autophagosomes was observed. Together, these studies suggest a role for Vps13 in the sequestration of the ER into autophagosomes at late endosomes. These observations may have important implications for understanding Parkinson's and other neurological diseases.
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http://dx.doi.org/10.1073/pnas.2008923117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414049PMC
August 2020

ROCK1 knockdown inhibits non-small-cell lung cancer progression by activating the LATS2-JNK signaling pathway.

Aging (Albany NY) 2020 06 17;12(12):12160-12174. Epub 2020 Jun 17.

Department of Cardiology, Tianjin First Central Hospital, Tianjing 300192, P.R. China.

Rho-associated kinase 1 (ROCK1) regulates tumor metastasis by maintaining cellular cytoskeleton homeostasis. However, the precise role of ROCK1 in non-small-cell lung cancer (NSCLC) apoptosis remains largely unknown. In this study, we examined the function of ROCK1 in NSCLS survival using RNA interference-mediated knockdown. Our results showed that ROCK1 knockdown reduced A549 lung cancer cell viability . It also inhibited A549 cell migration and proliferation. Transfection of ROCK1 siRNA was associated with increased expression of large tumor suppressor kinase 2 (LATS2) and c-Jun N-terminal kinase (JNK). Moreover, ROCK1 knockdown-induced A549 cell apoptosis and inhibition of proliferation were suppressed by LATS2 knockdown or JNK inactivation, suggesting that ROCK1 deficiency triggers NSCLC apoptosis in a LATS2-JNK pathway-dependent manner. Functional analysis further demonstrated that ROCK1 knockdown dysregulated mitochondrial dynamics and inhibited mitochondrial biogenesis. This effect too was reversed by LATS2 knockdown or JNK inactivation. We have thus identified a potential pathway by which ROCK1 downregulation triggers apoptosis in NSCLC by inducing LATS2-JNK-dependent mitochondrial damage.
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http://dx.doi.org/10.18632/aging.103386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343464PMC
June 2020

Chemical characteristics and cytoprotective activities of polysaccharide fractions from Athyrium Multidentatum (Doll.) Ching.

Int J Biol Macromol 2020 May 10. Epub 2020 May 10.

Department of Pharmacy, Weifang Medical University, Weifang 261053, PR China.

Five polysaccharide fractions (PS-1, PS-2, PS-3, PS-4 and PS-5) were successfully isolated from Athyrium Multidentatum (Doll.) Ching by anion-exchange column chromatography. Their in vitro cytoprotective activities and the underlying mechanisms were explored in this paper. Chemical analysis suggested that the five polysaccharide fractions were heteropolysaccharides with different molecular weights and monosaccharide compositions. Treatment with these polysaccharide fractions could increase cell viabilities, superoxide dismutase/catalase activities, nitric oxide contents, mitochondrial membrane potential levels and Bcl-2/Bax ratios, and reduce cell apoptosis, intracellular reactive oxygen species production and malondialdehyde contents in HO-damaged cells. Moreover, these polysaccharide fractions enhanced the mRNA expression levels of PI3K, Akt, FOXO3a, Nrf2 and HO-1 and PS-4 exhibited the most powerful effects on the mRNA expression of these genes. Current findings suggested that the polysaccharide fractions decreased HO-induced apoptosis of HUVECs. The activation of PI3K/Akt/FOXO3a and Nrf2/HO-1 signaling pathways might be involved in the protective mechanisms of the active fractions. The polysaccharides might be one of the key bioactive ingredients of Athyrium Multidentatum (Doll.) Ching for the treatment of oxidative damage.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.053DOI Listing
May 2020

Remarkable phosphate removal and recovery from wastewater by magnetically recyclable LaOCO/γ-FeO nanocomposites.

J Hazard Mater 2020 10 24;397:122597. Epub 2020 Apr 24.

College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, China.

Owing to the twin problems of eutrophication and global phosphorus (P) scarcity, the removal and recovery of phosphate from water and wastewater have received increasing attention. Herein, magnetically recyclable LaOCO/γ-FeO adsorbents were rationally designed by derivation from La/Fe binary metal organic framework (MOF) precursors via calcination treatment. Based upon preliminary screening of as-prepared LaOCO/γ-FeO nanocomposites with different La-to-Fe molar ratios in terms of phosphate sorption capacity and magnetic property as well as La content, LaOCO/γ-FeO nanocomposite with a La-to-Fe molar ratio of 2:1 was selected for further characterization and adsorption performance evaluation. Batch adsorption experiments showed that LaOCO/γ-FeO (2:1) adsorbent exhibited a remarkable phosphate sorption capacity of 134.82 mg P/g, a fast sorption kinetic, strong selectivity for phosphate in the presence of co-existing anions, and a wide applicable pH range of 3-9. Furthermore, LaOCO/γ-FeO (2:1) sorbent displayed an excellent sorption performance for low-concentration wastewater, a low dosage of 0.1 g/L was sufficiently enough for reducing P-concentration from 0.5 mg P/L to below 10 μg P/L within 20 min. In a real sewage of 2.68 mg P/L, 0.2 g/L of sorbent could reduce the concentration of phosphate to <0.01 mg P/L within 50 min. Moreover, over 83.1 % of original sorption capacity could be retained after 5 consecutive regeneration cycles, showing great regenerative performance of the adsorbent. These development is expected to be meaningful for practical water purification.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122597DOI Listing
October 2020