Publications by authors named "Dongfang Wang"

191 Publications

Fabrication of Fe3O4/ZIF-8 nanocomposite for simultaneous removal of copper and arsenic from water/soil/swine urine.

J Environ Manage 2021 Apr 17;290:112626. Epub 2021 Apr 17.

Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People's Republic of China; Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People's Republic of China. Electronic address:

In this study, a magnetic nanocomposite (denoted as FZ) was fabricated using FeOand zeolitic imidazolate framework-8 (ZIF-8), based on a coprecipitation method. FZ could efficiently remove Cu(Cu(II)) and AsO (As(III)) ions simultaneously from water, soil, and swine urine samples through hydrogen bonding and electrostatic interactions. The Cu(II) and As(III) removal efficiencies of the optimal FZ sample increased gradually with time and reached 99.1% and 98.4%, respectively, in 180 min. The maximum adsorption capacities of FZ4 for Cu(II) and As(III) were determined to be 33.48 mg/g and 21.12 mg/g, respectively. Additionally, the FZ with a high saturation magnetization (49.8 emu/g) was easily recovered from aqueous solutions and soil samples. Furthermore, zebrafish experiments indicated that FZ possessed a high biosafety. Thus, this study introduces a promising method for treating water, soil, and swine urine samples contaminated with Cu(II) and As(III), and verifies that FZ is suitable for practical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.112626DOI Listing
April 2021

Predicting adsorption of micropollutants on non-functionalized and functionalized multi-walled carbon nanotubes: Experimental study and LFER modeling.

J Hazard Mater 2021 Jun 13;411:125124. Epub 2021 Jan 13.

Environmental Biotechnology National Research Laboratory, School of Chemical Engineering, Jeonbuk National University (formerly Chonbuk National University), 567 Beakje-dearo, Deokjin-gu, Jeonju, Jeonbuk 54896, Republic of Korea. Electronic address:

It is of great importance to predict the adsorption of micropollutants onto CNTs, which is not only useful for exploring their potential adsorbent applications, but also helpful for better understanding their fate and risks in aquatic environments. This study experimentally examined the adsorption affinities of thirty-one micropollutants on four multi-walled CNTs (MWCNTs) with different functional groups (non-functionalized, -COOH, -OH, and -NH). The properties of each adsorbent were predicted based on the linear free energy relationship (LFER) model. The experimental results showed that MWCNTs-COOH has remarkable adsorption affinities for positively charged compounds (1.996-3.203 log unit), whereas MWCNTs-NH has high adsorption affinities for negatively charged compounds (1.360-3.073 log unit). Regarding neutral compounds, there was no significant difference in adsorption affinities of all types of CNTs. According to modeling results, the adsorption affinity can be accurately predicted using LFER models with R in the range of 0.81-0.91. Based on the developed models, the adsorption mechanism and contribution of individual intermolecular interactions to the overall adsorption were interpreted. For non-functionalized MWCNTs, molecular interactions induced by molecular volume and H-bonding basicity predominantly contribute to adsorption, whereas for functionalized MWCNTs, the Coulombic interaction due to the charges is an important factor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125124DOI Listing
June 2021

Shift work disorder and related influential factors among shift workers in China.

Sleep Med 2021 May 13;81:451-456. Epub 2021 Mar 13.

Center for Public Health Initiatives, University of Pennsylvania, Philadelphia, PA, USA.

Background: Shift work may cause insomnia and sleepiness in individuals. The present study aimed to exam shift work disorder (SWD), and to investigate their associations with individual characteristics.

Methods: A total of 1833 shift workers were assessed using the Pittsburg Sleep Quality Index, Epworth Sleepiness Scale (ESS), Composite Scale of Morningness (CSM), Circadian Type Inventory (CTI), Center for Epidemiologic Studies-Depression Scale (CES-D), Beck Anxiety Inventory (BAI) and other self-compiled socio-demographic questionnaires.

Results: In the current sample, 17.1% shift workers have experienced insomnia symptoms, 20.9% were tested for daytime sleepiness, and 19.9% were categorized as having SWD. Logistics regressions revealed that history of mental disorders (OR = 2.04, 95% CI = 1.30-3.21), chronic physical illness (OR = 1.53, 95% CI = 1.17-1.99), CES-D scores (OR = 1.03, 95% CI = 1.02-1.05), BAI scores (OR = 1.04, 95% CI = 1.03-1.06), languid/vigorous tendencies (OR = 1.06, 95% CI = 1.03-1.10) were positively associated with the onset of SWD, while morningness (OR = 0.97, 95% CI = 0.94-0.99) decreased the odds of SWD onset.

Conclusions: These findings suggested that attention should be drawn to individuals with mental and chronic diseases in when scheduling work shifts. While SWD and its associates should be considered when providing psychological services to shift workers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sleep.2021.03.008DOI Listing
May 2021

Is returning to school during the COVID-19 pandemic stressful? A study on immediate mental health status of Chinese college students.

J Affect Disord 2021 05 19;287:261-267. Epub 2021 Mar 19.

Center for Public Health Initiatives, University of Pennsylvania, Philadelphia, PA, USA.

Background: As the COVID-19 pandemic has posed substantial impacts on individual's daily routine and psychological state. For the first time at great scale, Chinese college students had their educational activities moved online in spring 2020. Due to this unexpected isolation and unconventional learning method, their mental health following returning to school is worth investigating.

Methods: Between June 1 and June 15, 2020, a total of 8,921 returning college students' mental health status were assessed using instruments designed for psychiatric disorders, namely the 9-Item Patient Heath Questionnaire (PHQ-9), 7-Item Generalized Anxiety Disorder Scale (GAD-7), 6-Item Impact of Event Scale (IES-6), Youth Self Rating Insomnia Scale (YSIS), and self-developed questionnaire.

Results: Our results showed that 8.7%, 4.2%, 10.5%, and 6.1% of the participants experienced depression, anxiety, acute stress, and insomnia, respectively, with a total of 19.8% reporting having at least one psychiatric symptom following their return to school. Sophomore and Senior year, and presence of previous psychiatric conditions contribute to the increased occurrence of psychiatric issues. The level of impact by COVID-19 on one's daily functioning is also positively associated with poor mental health.

Conclusions: Our findings suggested no significant increase in the prevalence of psychiatric symptoms, following the first batch of students' return to school. These findings aim to complement the current understanding of the psychiatric impact of COVID-19 on students and assist school principals to plan their return-to-school approaches in a mental-health sensitive way.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jad.2021.03.035DOI Listing
May 2021

Changes in psychotic-like experiences and related influential factors in technical secondary school and college students during COVID-19.

Schizophr Res 2021 Mar 13;231:3-9. Epub 2021 Mar 13.

Department of Social Psychiatry, the Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, China. Electronic address:

Objective: Although students have been found to be at high risk of distress during the COVID-19 pandemic, little is known about the pandemic's impact on psychotic-like experiences (PLEs). We conducted a study in technical secondary school and college students before and during the pandemic to explore changes in PLEs and relevant influential factors.

Methods: A total of 938 students completed both waves of the survey through electronic questionnaires. PLEs were assessed using the 15-item Positive Subscale of the Community Assessment of Psychic Experiences (CAPE-P15). Childhood trauma, perceived stress, resilience, and demographic factors were evaluated at baseline, and psychological status was measured during the pandemic.

Results: The overall CAPE-P15 scores significantly decreased during the pandemic. Students with persistent PLEs showed the most severe COVID-19 related psychological symptoms, followed by new-onset and remitted individuals; those without PLEs exhibited the mildest symptoms (all p < .001). A single parent family (OR = 4.707), more childhood trauma (OR = 1.056), and a higher family income (OR = 1.658) were predictive of new-onset PLEs during the pandemic, while better resilience was a protective factor, associated with remission of previous PLEs (OR = 0.932).

Conclusions: Despite a downward trend in the prevalence of PLEs during the pandemic, PLEs predict greater serious psychological impact due to COVID-19, especially for students with persistent PLEs. Interventions that cultivate students' resilience are urgently needed to reduce PLEs and improve mental health, especially for students from single parent households or those who have experienced childhood trauma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.schres.2021.02.015DOI Listing
March 2021

Molecular cloning and characterization of an atypical butyrylcholinesterase-like protein in zebrafish.

Comp Biochem Physiol B Biochem Mol Biol 2021 Mar 2;255:110590. Epub 2021 Mar 2.

School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, Guangdong, China; Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia. Electronic address:

Cholinesterases act as bio scavengers to clear organophosphorus (OP) compounds and prodrugs. The butyrylcholinesterase (BChE) gene has been found in several types of teleost fish but this gene has yet to be identified in cyprinid fish. Indeed, BChE homologs have not been found in the zebrafish (Danio rerio) genomic database. Here, we demonstrate that BChE activity is present in zebrafish, in line with other groups' findings. Using in-gel native-PAGE enzymatic activity staining and LC-MS/MS technique, an atypical BChE-like protein was identified in zebrafish. The si:ch211-93f2.1 gene was cloned, and His-tagged recombinant protein was expressed using the Pichia yeast system. The purified protein (molecular weight ~ 180 kDa) showed BChE activity, and degraded acetylcholinesterase (ACh) at a higher rate than BCh. However, phylogram analysis shows that this novel cholinesterase shared an evolutionary origin with carboxylic esterase rather than BChE. The zebrafish BChE-like protein shares structural characteristics with cholinesterase and carboxylesterase. The 2-arachidonoylglycerol (2-AG), nicosulfuron, and triacetin exhibited a higher binding affinity to the zebrafish BChE-like protein than BCh and ACh. With the identification of BChE-like protein in zebrafish, this study could shed light on the origin of BChE and may contribute towards the development of a BChE knockout zebrafish model for sensitive drug or toxin screening.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpb.2021.110590DOI Listing
March 2021

iMAP: integration of multiple single-cell datasets by adversarial paired transfer networks.

Genome Biol 2021 Feb 18;22(1):63. Epub 2021 Feb 18.

BIOPIC and School of Life Sciences, Peking University, Beijing, China.

The integration of single-cell RNA-sequencing datasets from multiple sources is critical for deciphering cell-to-cell heterogeneities and interactions in complex biological systems. We present a novel unsupervised batch effect removal framework, called iMAP, based on both deep autoencoders and generative adversarial networks. Compared with current methods, iMAP shows superior, robust, and scalable performance in terms of both reliably detecting the batch-specific cells and effectively mixing distributions of the batch-shared cell types. Applying iMAP to tumor microenvironment datasets from two platforms, Smart-seq2 and 10x Genomics, we find that iMAP can leverage the powers of both platforms to discover novel cell-cell interactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13059-021-02280-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891139PMC
February 2021

Comparisons between self-reported and interview-verified psychotic-like experiences in adolescents.

Early Interv Psychiatry 2021 Feb 16. Epub 2021 Feb 16.

Department of Social Psychiatry, The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, China.

Aim: The 15-item positive subscale of the Community Assessment of Psychic Experiences (CAPE-P15) has been widely used for measuring self-reported psychotic-like experiences (PLEs). However, its validity has not been well established. This study aimed to explore the consistency of self-reported PLEs (PLEs-S) and interview-verified PLEs (PLEs-I) based on the same items of the CAPE-P15.

Methods: A total of 1255 college students completed the CAPE-P15 for measuring lifetime and current PLEs. Half of the students with high-risk scores and 5% of the rest were interviewed through telephone. Telephone interviews were based on the items of the CAPE-P15 using the symptom criteria for attenuated positive symptom syndrome.

Results: When considering the presence of PLEs only, all κ values and correspondence rates (CRs) fell below the thresholds. However, there was adequate consistency for lifetime PLEs when associated distress was also considered in self-report (κ = .432, CR = 90.0%). Among three factors, only bizarre experiences (BEs) showed adequate diagnostic accuracy in detecting lifetime PLEs when combined with distress. Cut-off points of 1.30 (sensitivity of 89.2% and specificity of 92.3%) and 1.57 (sensitivity of 79.2% and specificity of 73.8%) for frequency scores were found to best identify genuine PLEs during lifetime and in the past month, respectively.

Conclusions: Although the validity of the CAPE-P15 for genuine PLEs is unsatisfactory, the scale showed much better diagnostic accuracy when combined with associated distress, especially for detecting lifetime PLEs. Self-report items on BEs may be more sensitive and specific when identifying PLEs in late adolescence.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/eip.13132DOI Listing
February 2021

Comparative efficacy of pharmacological treatments on measures of self-rated functional outcomes using the Sheehan Disability Scale in patients with major depressive disorder: a systematic review and network meta-analysis.

CNS Spectr 2021 Feb 15:1-9. Epub 2021 Feb 15.

Mood Disorders Psychopharmacology Unit, Toronto Western Hospital, University Health Network, Toronto, Ontario, Canada.

Objective: More than 50% patients with major depressive disorder (MDD) have severe functional impairment. The restoration of patient functioning is a critical therapeutic goal among patients with MDD. We conducted a systematic review and network meta-analysis to evaluate the efficacy of pharmacological treatments on self-rated functional outcomes using the Sheehan Disability Scale in adults with MDD in randomized clinical trials.

Methods: PubMed, EMBASE, PsycINFO, Cochrane Library, and ClinicalTrials.gov were searched from inception to December 10, 2019. Summary statistics are reported as weighted mean differences with 95% confidence intervals. Interventions were ranked using the surface under the cumulative ranking probabilities.

Results: We included 42 randomized controlled trials (RCTs) (n = 18 998) evaluating the efficacy of 13 different pharmacological treatments on functional outcomes, as measured by the Sheehan Disability Scale (SDS). Duloxetine was the most effective pharmacological agent on functional outcomes, followed by (ranked by efficacy): paroxetine, levomilnacipran, venlafaxine, quetiapine, desvenlafaxine, agomelatine, escitalopram, amitriptyline, bupropion, sertraline, vortioxetine, and fluoxetine. Serotonin and norepinephrine reuptake inhibitors were more effective than other drug classes. Additionally, the comparison-adjusted funnel plot suggested the publication bias between small and large studies was relatively low.

Conclusions: Our results indicate that there may be differences across antidepressant agents and classes with respect to self-reported functional outcomes. Validation and replication of these findings in large-scale RCTs are warranted. Our research results will be clinically useful for guiding psychiatrists in treating patients with MDD and functional impairment. PROSPERO registration number CRD42018116663.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S1092852921000171DOI Listing
February 2021

Simultaneously removal of Cr(VI) and Cd(II) from water using a flower-like primary battery nanosystem.

Sci Total Environ 2021 Apr 8;765:142735. Epub 2020 Oct 8.

College of Environmental Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China. Electronic address:

In this study, a new flower-like primary battery nanosystem termed "Zn/CCP/ZIF-8" was prepared by depositing conductive carbon paint (CCP) and zeolitic imidazolate framework-8 (ZIF-8) on a zinc plate (Zn). Therein, CCP had good conductivity performance and adhesiveness, ZIF-8 and Zn/CCP/ZIF-8 possessed BET specific surface areas of 1909.5 and 1265.4 m/g respectively. The results showed that the Zn/CCP/ZIF-8 nanosystem could effectively simultaneously adsorb hexavalent chromium (Cr(VI)) and bivalent cadmium (Cd(II)) from water. The system could promote the transfer of electrons from Zn to Cr(VI) and Cd(II) which were effectively reduced to trivalent chromium (Cr(III)) and cadmium (Cd), respectively. The resultant Zn/CCP/ZIF-8/Cr/Cd composite was then easily separated from water. The adsorption isotherm, kinetics, and thermodynamics of the prepared Zn/CCP/ZIF-8 for Cr(VI) and Cd(II) were investigated. An electrochemistry performance test proved that the Zn/CCP/ZIF-8 system was a primary battery. Notably, the Zn/CCP/ZIF-8 system substantially reduced the amounts of Cr(VI) and Cd(II) absorbed by zebrafish and water spinach, thus increasing food safety. The results of a rat test indicated that the Zn/CCP/ZIF-8 system possessed a high biosafety. This study provides a promising, eco-friendly, and facile method for the simultaneously treatment of Cr(VI) and Cd(II) contamination of water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.142735DOI Listing
April 2021

Motivational differences in unipolar and bipolar depression, manic bipolar, acute and stable phase schizophrenia.

J Affect Disord 2021 03 2;283:254-261. Epub 2021 Feb 2.

Department of psychiatry, Brains Hospital of Hunan province, Changsha, China. Electronic address:

Background: Motivational anhedonia has been observed in patients with a wide range of mental disorders. However, the similarity and uniqueness of this deficit across diagnostic groups has not been thoroughly investigated.

Method: The study compared motivational deficits in 37 patients with major depressive disorder (MDD), 32 with bipolar depression, 33 with manic bipolar disorder (BD), 30 with acute phase and 33 with stable phase schizophrenia, as well as 47 healthy controls. Participants were administered the Effort-Expenditure for Reward Task which measures allocation of effort between a high-effort and a low-effort task for monetary rewards at varying magnitudes and probabilities.

Results: Compared with healthy controls, BD manic, acute and stable phase schizophrenia patients were significantly less likely to choose the high-effort task in the high reward magnitude condition. BD manic and acute phase schizophrenia patients were significantly less likely to choose the high-effort task in the high probability condition. Acute and stable phase schizophrenia patients made less effort in the high estimated value condition. Bipolar manic patients made excessive effort in low estimated value but less effort in high estimated value. Contrary to expectations, both the unipolar and bipolar depression patients did not differ significantly from healthy controls in reward magnitude, probability, and estimated value conditions. Anhedonia and negative symptoms were associated with fewer high-effort task choices in schizophrenia patients.

Conclusion: Motivation anhedonia showed distinct patterns across psychiatric patients: acute phase schizophrenia was the most severely affected, bipolar mania was similar to schizophrenia, but bipolar depression was similar to unipolar depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jad.2021.01.075DOI Listing
March 2021

Programmable Transformations of DNA Origami Made of Small Modular Dynamic Units.

J Am Chem Soc 2021 Feb 2;143(5):2256-2263. Epub 2021 Feb 2.

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322, United States.

Dynamic DNA origami has been employed for generating a rich repository of molecular nanomachines that are capable of sensing various cues and changing their conformations accordingly. The common design principle of the existing DNA origami nanomachines is that each dynamic DNA origami is programmed to transform in a specific manner, and the nanomachine needs to be redesigned to achieve a different form of transformation. However, it remains challenging to enable a multitude of controlled transformations in a single design of dynamic DNA nanomachine. Here we report a modular design method to programmatically tune the shapes of a DNA origami nanomachine. The DNA origami consists of small, modular DNA units, and the length of each unit can be selectively changed by toehold-mediated strand displacement. By use of different combinations of trigger DNA strands, modular DNA units can be selectively transformed, leading to the programmable reconfiguration of the overall dimensions and curvatures of DNA origami. The modular design of programmable shape transformation of DNA origami can find potential applications in more sophisticated molecular nanorobots and smart drug delivery nanocarriers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c10576DOI Listing
February 2021

Dysregulation of kynurenine pathway and potential dynamic changes of kynurenine in schizophrenia: A systematic review and meta-analysis.

Neurosci Biobehav Rev 2021 Apr 26;123:203-214. Epub 2021 Jan 26.

NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China; Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China. Electronic address:

The kynurenine (KYN) pathway is postulated to play various roles in immune system dysregulation of schizophrenia (SCZ). We conducted a meta-analysis to explore the association between six key metabolites of KYN pathway (i.e., tryptophan (TRP), KYN, quinolinic acid (QUIN), and kynurenic acid (KYNA)) and SCZ. Priori Bonferroni adjustments were conducted for multiple comparisons. In total, 42 studies that examined the relationship between the metabolites in KYN pathway mentioned above and SCZ in 4217 participants and nine studies that examined alterations of these metabolites after antipsychotic treatments were included. The results demonstrate that (1) subjects with prescribed medication had significantly higher KYN levels when compared to controls; (2) higher KYN levels in cerebrospinal fluid (CSF), lower plasma KYN levels and higher CSF KYNA levels were associated with SCZ; (3) the KYN levels were higher in subjects with SCZ after antipsychotic treatments when compared with baseline. The evidence provides valuable insight of the potential underlying involvement of the KYN pathway in the pathogenesis of SCZ.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neubiorev.2021.01.018DOI Listing
April 2021

Evaluation of Recirculation During Venovenous Extracorporeal Membrane Oxygenation Using Computational Fluid Dynamics Incorporating Fluid-Structure Interaction.

ASAIO J 2020 Dec 9. Epub 2020 Dec 9.

Department of Surgery, University of Kentucky, Lexington, KT.

Recirculation in venovenous extracorporeal membrane oxygenation (VV ECMO) leads to reduction in gas transfer efficiency. Studies of the factors contributing have been performed using in vivo studies and computational models. The fixed geometry of previous computational models limits the accuracy of results. We have developed a finite element computational fluid dynamics model incorporating fluid-structure interaction (FSI) that incorporates atrial deformation during atrial filling and emptying, with fluid flow solved using large eddy simulation. With this model, we have evaluated an extensive number of factors that could influence recirculation during two-site VV ECMO, and characterized their impact on recirculation, including cannula construction, insertion depth and orientation, VV ECMO configuration, circuit blood flow, and changes in volume, venous return, heart rate, and blood viscosity. Simulations revealed that extracorporeal blood flow relative to cardiac output, ratio of superior vena caval (SVC) to inferior vena caval (IVC) blood flow, position of the SVC cannula relative to the cavo-atrial junction, and orientation of the return cannula relative to the tricuspid valve had major influences (>20%) on recirculation fraction. Factors with a moderate influence on recirculation fraction (5%-20%) include heart rate, return cannula diameter, and direction of extracorporeal flow. Minimal influence on recirculation (<5%) was associated with atrial volume, position of the IVC cannula relative to the cavo-atrial junction, the number of side holes in the return cannula, and blood viscosity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MAT.0000000000001314DOI Listing
December 2020

Mental Health Among College Students During the COVID-19 Pandemic in China: A 2-Wave Longitudinal Survey.

J Affect Disord 2021 02 24;281:597-604. Epub 2020 Nov 24.

Center for Public Health Initiatives, University of Pennsylvania, Philadelphia, PA, USA.

Background: Growing evidence supports a clear association between COVID-19 pandemic and mental health. However, little is known about the longitudinal course of psychopathology in young adults at different stages of the pandemic.

Methods: This large-scale, longitudinal, population-based survey was conducted among college students in China. The rates of three mental health problems (acute stress, anxiety, and depressive symptoms), and their change patterns at two phases of the pandemic (early vs under-control) were measured. Predictors of changes in mental health symptoms were examined utilizing multivariate regression.

Results: Among the 164,101 college students who participated in the first wave survey (T1=during onset of outbreak), 68,685 (41.9%) completed a follow-up survey (T2=during remission). In the follow-up survey, the prevalence of probable acute stress (T1: 34.6%; T2: 16.4%) decreased, while the rates of depressive (T1: 21.6%; T2: 26.3%) and anxiety symptoms (T1: 11.4%; T2: 14.7%) increased. Senior students, with suspected or conformed cases in their community and COVID-19 related worries (all AORs > 1.20, ps < 0.001) were found to have a higher risk of developing mental health problems in at least one wave. Less physical exercise, low perceived social support, and a dysfunctional family were found to negatively impact psychological symptoms.

Conclusions: Acute stress, anxiety, and depressive symptoms have been prevalent among college students during the COVID-19 epidemic, and showed a significant increase after the initial stage of the outbreak. Some college students, especially those with the risk factors noted above, exhibited persistent or delayed symptoms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jad.2020.11.109DOI Listing
February 2021

Targeted In Vivo Delivery of NF-κB Decoy Inhibitor Augments Sensitivity of B Cell Lymphoma to Therapy.

Mol Ther 2021 03 26;29(3):1214-1225. Epub 2020 Nov 26.

Department of Immuno-Oncology, Beckman Research Institute, City of Hope, Duarte, CA, USA. Electronic address:

Despite recent advances, non-Hodgkin's B cell lymphoma patients often relapse or remain refractory to therapy. Therapeutic resistance is often associated with survival signaling via nuclear factor κB (NF-κB) transcription factor, an attractive but undruggable molecular target. In this study, we describe a bipartite inhibitor comprising a NF-κB-specific decoy DNA tethered to a CpG oligodeoxynucleotide (ODN) targeting Toll-like receptor-9-expressing B cell lymphoma cells. The Bc-NFκBdODN showed efficient uptake by human diffuse large B cell (U2932, OCI-Ly3), Burkitt (RaJi), and mantle cell (Jeko1) lymphomas, respectively. We confirmed that Bc-NFκBdODN inhibited NF-κB nuclear translocation and DNA binding, resulting in CCND2 and MYC downregulation. Bc-NFκBdODN enhanced radiosensitivity of lymphoma cells in vitro. In xenotransplanted human lymphoma, local injections of Bc-NFκBdODN reduced NF-κB activity in whole tumors. When combined with a local 3-Gy dose of radiation, Bc-NFκBdODN effectively arrested OCI-Ly3 lymphoma progression. In immunocompetent mice, intratumoral injections of Bc-NFκBdODN suppressed growth of directly treated and distant A20 lymphomas, as a result of systemic CD8 T cell-dependent immune responses. Finally, systemic administration of Bc-NFκBdODN to mice bearing disseminated A20 lymphoma induced complete regression and extended survival of most of the treated mice. Our results underscore clinical relevance of this strategy as monotherapy and in support of radiation therapy to benefit patients with resistant or relapsed B cell lymphoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymthe.2020.11.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934632PMC
March 2021

Programming the Curvatures in Reconfigurable DNA Domino Origami by Using Asymmetric Units.

Nano Lett 2020 11 23;20(11):8236-8241. Epub 2020 Oct 23.

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322, United States.

The DNA origami technique is a robust method for the design of DNA nanostructures with prescribed shapes, including complex curved geometries. In addition to static structures, dynamic DNA origami has been used to construct sophisticated nanomachines that can reconfigure their shapes in response to external stimuli. Here, we report a new method to design DNA origami structures that can transform between a noncurved conformation and curved conformation. The reconfigurable structures are developed on the basis of dynamic DNA domino origami, which can transform in a cascading process initiated by trigger DNA strands. The degree of curvature could be programmed by tuning the sizes of DNA units within the origami.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.0c03348DOI Listing
November 2020

Effective adsorption of mercury by Zr(IV)-based metal-organic frameworks of UiO-66-NH from aqueous solution.

Environ Sci Pollut Res Int 2021 Feb 6;28(6):7068-7075. Epub 2020 Oct 6.

College of Resources and Environmental Science, South-Central University for Nationalities, Wuhan, 430074, China.

In this study, Zr-based metal-organic frameworks (MOFs) of UiO-66 and UiO-66-NH were synthesized and applied to removal of mercury from aqueous solution. The characterizations of UiO-66 and UiO-66-NH were examined by X-ray diffraction (XRD), scanning electron microscope (SEM), and thermogravimetric analysis (TGA). To investigate the adsorption properties of UiO-66-NH for mercury, the experiments of kinetics, isotherm, pH, temperature, and salt concentration were conducted, and the results were compared with those by UiO-66. The result showed that UiO-66-NH has a higher adsorption capacity for mercury than UiO-66. The maximum adsorption capacity of UiO-66-NH was 223.8 ± 17.8 mg g at 313 K. The salt concentration of NaCl has a significant effect on the adsorption of mercury on UiO-66, while UiO-66-NH can maintain the stable adsorption capacity for mercury in the concentration range of 0.1-0.5 M NaCl. Adsorption thermodynamics result indicated that the adsorption process of mercury on UiO-66-NH was spontaneous and endothermic. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses showed that the mercury was successfully adsorbed on the surface of UiO-66-NH and amino functional group as a soft base played a major role to react with mercury during the adsorption process. Graphical abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-11080-9DOI Listing
February 2021

The efficacy assessment of convalescent plasma therapy for COVID-19 patients: a multi-center case series.

Signal Transduct Target Ther 2020 10 6;5(1):219. Epub 2020 Oct 6.

National Engineering Research Center of Immunological, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing, P.R. China.

Convalescent plasma (CP) transfusion has been indicated as a promising therapy in the treatment for other emerging viral infections. However, the quality control of CP and individual variation in patients in different studies make it rather difficult to evaluate the efficacy and risk of CP therapy for coronavirus disease 2019 (COVID-19). We aimed to explore the potential efficacy of CP therapy, and to assess the possible factors associated with its efficacy. We enrolled eight critical or severe COVID-19 patients from four centers. Each patient was transfused with 200-400 mL of CP from seven recovered donors. The primary indicators for clinical efficacy assessment were the changes of clinical symptoms, laboratory parameters, and radiological image after CP transfusion. CP donors had a wide range of antibody levels measured by serology tests which were to some degree correlated with the neutralizing antibody (NAb) level. No adverse events were observed during and after CP transfusion. Following CP transfusion, six out of eight patients showed improved oxygen support status; chest CT indicated varying degrees of absorption of pulmonary lesions in six patients within 8 days; the viral load was decreased to a negative level in five patients who had the previous viremia; other laboratory parameters also tended to improve, including increased lymphocyte counts, decreased C-reactive protein, procalcitonin, and indicators for liver function. The clinical efficacy might be associated with CP transfusion time, transfused dose, and the NAb levels of CP. This study indicated that CP might be a potential therapy for severe patients with COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-020-00329-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538052PMC
October 2020

The Effect Mechanism of Fe on Coal Pyrolysis to NO Precursors: Quantum Chemical Calculations and Mass Spectrometry Experiments.

ACS Omega 2020 Sep 31;5(36):23247-23256. Epub 2020 Aug 31.

School of Energy and Environment, Anhui University of Technology, Maanshan, Anhui 243002, China.

Density functional theory is adopted to thoroughly analyze the influence mechanism of Fe on the formation of NH and HCN. The structure of Fe adsorbed on the surface of seven-membered zigzag coal containing pyridine nitrogen is selected as the Fe-containing coal model. The effect of Fe on the nitrogen distribution during Zhundong coal pyrolysis is further studied by thermogravimetry-mass spectrometry. The theoretical calculations show that Fe increases the Mulliken charge density on the N5 surface, which increases the rate-determining step energy barrier value of NH generated from coal pyrolysis and inhibits the NH formation. On the other hand, Fe significantly enhances the bonding energy between σ N5-C6 and π N5-C6, increases the activation energy required for N stripping from the pyridine ring (about 69.14 kJ/mol higher than that without Fe), and inhibits HCN formation. The experimental results show that Fe catalyzes the precipitation peaks of NH and CHCN about 20 K ahead of time and has no obvious catalytic effect on HCN and HNCO. In terms of the nitrogen distribution, Fe significantly promotes the CHCN formation and shows a significant inhibitory effect on NH, HCN, and HNCO. Kinetic results show that Fe reduces the precipitation rates of NH and HCN, and the inhibitory effect on HCN is more significant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c02994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495788PMC
September 2020

Expanded Polytetrafluoroethylene/Graphite Composites for Easy Water/Oil Separation.

ACS Appl Mater Interfaces 2020 Aug 12;12(34):38241-38248. Epub 2020 Aug 12.

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin 53715, United States.

Oil spills in the ocean greatly threaten local environments, marine creatures, and coastal economies. An automatic water/oil separation material system was proposed in this study, and a tubular geometry was chosen to demonstrate the water/oil separation efficiency and effectiveness. The water/oil separation tubes were made of expanded polytetrafluoroethylene (ePTFE) and graphite composites. The permeation pressures of water and oil through the tube walls were tuned by adjusting the ePTFE microstructure, which, in turn, depended on the degree of expansion and the graphite content. Fourier-transform infrared spectroscopy was performed to confirm the compositions of the ePTFE/graphite composites, and a scanning electron microscope was used to examine the microstructure and morphology of the expanded PTFE/graphite composite tubes. When a proper pressure was applied, which was higher than the oil's permeation pressure (3.0 kPa) but lower than the water's permeation pressure (57 kPa), the oil leaked out of the tube walls while the water went through the ePTFE/graphite tubes. As such, the water/oil mixture could be separated and collected in different containers or an outer tube. Due to this automatic separation, the whole process could be done continuously and conveniently, thus exhibiting great potential in the practical applications of oil spill and water separation/remediation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c11583DOI Listing
August 2020

Expanded Polytetrafluoroethylene/Graphite Composites for Easy Water/Oil Separation.

ACS Appl Mater Interfaces 2020 Aug 12;12(34):38241-38248. Epub 2020 Aug 12.

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin 53715, United States.

Oil spills in the ocean greatly threaten local environments, marine creatures, and coastal economies. An automatic water/oil separation material system was proposed in this study, and a tubular geometry was chosen to demonstrate the water/oil separation efficiency and effectiveness. The water/oil separation tubes were made of expanded polytetrafluoroethylene (ePTFE) and graphite composites. The permeation pressures of water and oil through the tube walls were tuned by adjusting the ePTFE microstructure, which, in turn, depended on the degree of expansion and the graphite content. Fourier-transform infrared spectroscopy was performed to confirm the compositions of the ePTFE/graphite composites, and a scanning electron microscope was used to examine the microstructure and morphology of the expanded PTFE/graphite composite tubes. When a proper pressure was applied, which was higher than the oil's permeation pressure (3.0 kPa) but lower than the water's permeation pressure (57 kPa), the oil leaked out of the tube walls while the water went through the ePTFE/graphite tubes. As such, the water/oil mixture could be separated and collected in different containers or an outer tube. Due to this automatic separation, the whole process could be done continuously and conveniently, thus exhibiting great potential in the practical applications of oil spill and water separation/remediation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c11583DOI Listing
August 2020

Proximity-Induced Pattern Operations in Reconfigurable DNA Origami Domino Array.

J Am Chem Soc 2020 08 12;142(34):14566-14573. Epub 2020 Aug 12.

Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Molecular patterns with nanoscale precision have been used to mimic complex molecular networks. One key challenge in molecular patterns is to perform active pattern operations in controllable systems to fully imitate their complex dynamic behaviors. Here, we present a reconfigurable DNA origami domino array-based dynamic pattern operation (DODA DPO) system to perform proximity-induced molecular control for complex pattern operations. The activatable platform of reconfigurable DODA endows a spontaneous cascade of stacking conformational transformation from the "before" to the "after" conformation by a set of "trigger" DNA strands. The conformational transformation further brings the operational pattern units into close proximity to undergo DNA strand displacement cascades to accomplish three different pattern operations of "writing", "erasing", and "shifting". Our results also demonstrate the reconfigurable DODA DPO system provides a useful basis to study various molecular control analysis in a fully programmable and controllable fashion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.0c06061DOI Listing
August 2020

Biologically Functionalized Expanded Polytetrafluoroethylene Blood Vessel Grafts.

Biomacromolecules 2020 09 21;21(9):3807-3816. Epub 2020 Aug 21.

Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.

Cardiovascular diseases plague human health because of the lack of transplantable small-diameter blood vessel (SDBV) grafts. Although expanded polytetrafluoroethylene (ePTFE) has the potential to be used as a biocompatible material for SDBV grafts, long-term patency is still the biggest challenge. As discussed in this paper, by virtue of a novel material formulation and a new and benign alcohol/water lubricating agent, biofunctionalized ePTFE blood vessel grafts aimed at providing long-term patency were fabricated. Compared to the most prevalent modification of PTFE, namely surface treatment, this method realized bulk treatment, which could guarantee homogeneous and long-lasting performance throughout PTFE products. These blood vessel grafts included embedded functional biomolecules, such as arginylglycylaspartic acid, heparin, and selenocystamine, using water as a solvent in paste extrusion and in the expansion of ePTFE. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscope results confirmed the existence of these targeting biomolecules in the as-fabricated ePTFE blood vessel grafts. Meanwhile, the greatly improved biological functions of the grafts were demonstrated live and dead assays, cell morphology, CD31 staining, nitric oxide (NO) release, and anticoagulation tests. This novel and benign material formulation and fabrication method provides an opportunity to produce multibiofunctional ePTFE blood vessel grafts in a single step, thus yielding a potent product with significant commercial and clinical potential.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biomac.0c00897DOI Listing
September 2020

Characterising phospholipids and free fatty acids in patients with schizophrenia: A case-control study.

World J Biol Psychiatry 2020 Jul 17:1-14. Epub 2020 Jul 17.

School of Psychology and Key Laboratory of Cognition and Personality (Ministry of Education), Southwest University, Chongqing, P. R. China.

Objectives: Previous studies have indicated that schizophrenia (SCZ) is linked to abnormal phospholipid and fatty acid metabolism. However, comprehensive analysis of phospholipids and free fatty acids (FFAs) for SCZ is very limited. Herein, we sought to compare serum levels of phospholipids and FFAs between patients with SCZ and healthy controls (HCs).

Methods: One hundred and nineteen SCZ patients and 109 HCs were enrolled in the study. The levels of 177 phospholipids and FFAs were measured in serum samples using a targeted liquid chromatography-mass spectrometry (LC-MS)-based platform.

Results: One hundred and ten metabolites, including 16 FFAs, 25 phosphatidylcholines, 23 lysophosphatidylcholines, 11 phosphatidylcholine plasmalogens, 7 phosphatidylethanolamines, 9 lysophosphatidylethanolamines, 6 phosphatidylethanolamine plasmalogens, and 13 sphingomyelins, were observed to be significantly altered in SCZ patients compared to HCs. These disturbances may represent underlying pathophysiology, including but not limited to altered activity of phospholipases and acyltransferases, increased oxidative stress, dysfunctional oligodendrocyte glycosynapses, and elevated lipid mobilisation and β-oxidation.

Conclusions: Our findings suggest that complex lipid profile abnormalities are associated with SCZ. This study may contribute to investigating the role of phospholipid and FFA alterations in the pathoetiology of SCZ.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15622975.2020.1769188DOI Listing
July 2020

Trajectory and Functional Analysis of PD-1 CD4CD8 T Cells in Hepatocellular Carcinoma by Single-Cell Cytometry and Transcriptome Sequencing.

Adv Sci (Weinh) 2020 Jul 18;7(13):2000224. Epub 2020 May 18.

National Center for Liver Cancer Shanghai 200438 China.

The spatial heterogeneity of immune microenvironment in hepatocellular carcinoma (HCC) remains elusive. Here, a single-cell study involving 17 432 600 immune cells of 39 matched HCC (T), nontumor (N), and leading-edge (L) specimens by mass cytometry is conducted. The tumor-associated CD4/CD8 double-positive T (DPT) cells are found enriched in L regions with synergetic expression of PD-1/HLA-DR/ICOS/CD45RO and exhibit a higher level of IFN-, TNF-, and PD-1 upon stimulation. The enrichment of DPT and PD-1DPT in L regions indicates favorable prognosis. These tumor-associated DPT cells with similar phenotype are also verified in other tumors and HCC animal models. Single-cell RNA-seq further characterizes the molecular features of DPT cells and uncovers 11 clusters with different cytotoxicity, exhaustion, and activation scores. TCR-based trajectory analysis reveals that tumor-associated DPT clusters share separated ancestries with local CD4 or CD8SPT cells rather than CD3PBMC cells. TCR clones with frequency above 10 are mainly found coexisting in DPT and CD8SPT cells. Specifically, PD-1DPT cluster (TDPT_10) shares the same ancestry with exhausted CD8SPT cluster (TCD8T_2) and shows higher expression similarity and closer pathological location to PD-1CD8 than PD-1CD4T cells. Together, this study systematically characterizes the unique distribution of PD-1DPTs in HCC and puts forward new insights for the function and origin of tumor-associated DPT cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202000224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341083PMC
July 2020

A novel human acute encephalitis caused by pseudorabies virus variant strain.

Clin Infect Dis 2020 Jul 15. Epub 2020 Jul 15.

Department of Neurology, People's Hospital of Zhengzhou University, Henan People's Hospital, Zhengzhou, Henan, China.

Background: Pseudorabies virus (PRV) is a common pathogen in multiple animal species particularly in pigs. However, PRV infection in humans is rare and to the best of knowledge, PRV has never been isolated from human cases before.

Methods: Four acute encephalitis cases in humans were confirmed as PRV infection based on clinical symptoms, laboratory diagnosis, and metagenomic next-generation sequencing (mNGS). Cerebrospinal fluid (CSF) samples were collected and applied for virus isolation. Etiological and genetic characteristics of this PRV human isolate were further determined.

Results: The patients manifested respiratory dysfunction and acute neurological symptoms. The mNGS revealed PRV specific nucleotide sequences in patients' CSF samples (7-6198 reads and 0.2446%-80.58% coverage). The PRV envelope glycoprotein B antibody, glycoprotein E antibody, and neutralizing antibody were positively detected. For the first time, a PRV strain, designated hSD-1/2019, was isolated and identified from one CSF sample, and transmission electron microscopy revealed hSD-1/2019 had typical morphology similar to swine PRV. Phylogenetic analysis illustrated that hSD-1/2019 was genetically closest to those PRV variant strains currently circulating in pigs in China, and this strain showed similar etiological characteristics to Chinese PRV variant strains, while different from Chinese classical strain. Moreover, hSD-1/2019 showed high pathogenicity and induced acute neurological symptoms in pigs.

Conclusions: A PRV strain was isolated from an acute human encephalitis case. This isolate showed close phylogenetic relationships and similar etiological characteristics to Chinese PRV variant strains, implying the great risk of PRV transmission from pigs to humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/cid/ciaa987DOI Listing
July 2020

Association between arginine catabolism and major depressive disorder: A protocol for the systematic review and meta-analysis of metabolic pathway.

Medicine (Baltimore) 2020 Jul;99(27):e21068

Key Laboratory of Cognition and Personality, Faculty of Psychology, Ministry of Education.

Background: Alterations in the levels of arginine and its related catabolic products (ie, ornithine, citrulline, and argininosuccinate) in the urea and nitric oxide cycles were reported to play roles in the pathogenesis of major depressive disorder (MDD). The aim of this meta-analysis study is to explore the associations between arginine with its related catabolic products and MDD, and to discuss the possible role of arginine catabolism in the pathoetiology of MDD.

Methods: This study will be conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The English language literature published in the databases of PubMed, EMBASE, PsycINFO and Web of Science will be systematically searched. Forest plots will be used to estimate the associations between arginine and its related catabolic products with MDD. Subgroup analysis and meta-regression will also be performed to investigate the source of the potential heterogeneity. Sensitivity analysis will be performed to strengthen the results and to investigate whether any single study would have a significant effect on the results of meta-analysis. Publication bias will be tested for using the funnel plot with Begg test and Egger test. The Newcastle-Ottawa Scale will be applied to assess the risk of bias of observational studies.

Results: An integrated assessment of arginine with its related catabolic products may contribute to predict the risk of MDD.

Ethics And Dissemination: The results of associations between arginine with its related catabolic products and MDD will be reported in a peer-reviewed publication. With our findings from this meta-analysis, we hope to provide the most up-to-date evidence for the contributions of arginine and related catabolic products to predict the risk of MDD.

Systematic Review Registration: The protocol of current meta-analysis has been registered at the Open Science Framework [Available at: https://doi.org/10.17605/osf.io/7fn59].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000021068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337538PMC
July 2020

An entropy-based metric for assessing the purity of single cell populations.

Nat Commun 2020 06 22;11(1):3155. Epub 2020 Jun 22.

School of Life Sciences, BIOPIC and Beijing Advanced Innovation Centre for Genomics, Peking University, Beijing, China.

Single-cell RNA sequencing (scRNA-seq) is a versatile tool for discovering and annotating cell types and states, but the determination and annotation of cell subtypes is often subjective and arbitrary. Often, it is not even clear whether a given cluster is uniform. Here we present an entropy-based statistic, ROGUE, to accurately quantify the purity of identified cell clusters. We demonstrate that our ROGUE metric is broadly applicable, and enables accurate, sensitive and robust assessment of cluster purity on a wide range of simulated and real datasets. Applying this metric to fibroblast, B cell and brain data, we identify additional subtypes and demonstrate the application of ROGUE-guided analyses to detect precise signals in specific subpopulations. ROGUE can be applied to all tested scRNA-seq datasets, and has important implications for evaluating the quality of putative clusters, discovering pure cell subtypes and constructing comprehensive, detailed and standardized single cell atlas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-16904-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308400PMC
June 2020

Medication adherence and its correlates among patients affected by schizophrenia with an episodic course: A large-scale multi-center cross-sectional study in China.

Asian J Psychiatr 2020 Oct 8;53:102198. Epub 2020 Jun 8.

Department of Psychiatry, School of Medicine, Yale University, New Haven, CT, USA.

The Medication Adherence Rating Scale (MARS) is a fast, non-intrusive way of measuring adherence to medication in order to improve the management of patients with schizophrenia. The current cross-sectional study evaluated the reliability of the Chinese (Mandarin) version of the MARS and explored clinical and demographic correlates to medication adherence in a large sample of patients affected by schizophrenia with an episodic course in China. 1198 patients were recruited from 37 different hospitals in 17 provinces/municipalities of China and evaluated with the Medication Adherence Rating Scale (MARS), Clinical Global Impression-Severity of Illness (CGI-SI) and Sheehan Disability Scale-Chinese version (SDS-C). The MARS showed good internal consistency; Cronbach's alpha of total MARS was 0.83. Among the cohort of patients affected by schizophrenia with an episodic course, 28.5 % met the criteria of good adherence to antipsychotic medication; age, steady income, and severity of illness had significant effects on medication adherence. Medication adherence of patients affected by schizophrenia in mainland China was found to be relatively low, calling for urgent attention and intervention. Risk factors for non-adherence to medication among patients affected by schizophrenia with an episodic course include older age, unsteady income, being in the acute period of the disease, and severity of illness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajp.2020.102198DOI Listing
October 2020