Publications by authors named "Dongdong Xu"

137 Publications

Antiviral Treatment for Hepatitis C Is Associated With a Reduced Risk of Periprosthetic Joint Infection: A Meta-Analysis of Observational Studies.

J Arthroplasty 2022 Apr 26. Epub 2022 Apr 26.

Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Shanghai Sixth People's Hospital, Shanghai, The People's Republic of China.

Background: Recently, preoperative antiviral therapies for hepatitis C virus (HCV) have become available for total joint arthroplasty (TJA) patients. The objective of this meta-analysis is to investigate the impact of anti-HCV treatment on the incidence of postoperative complications after primary TJAs.

Methods: We searched PubMed, EMBASE, Scopus, Web of Science, and Cochrane Library databases for relevant studies from inception to March 5, 2022. The pooled odds ratios with 95% CI of the risk of postoperative complications were calculated using the random effects model. Subgroup analyses were conducted on the basis of surgery type, antiviral regimes, and duration of follow-up.

Results: Eight retrospective cohort studies fulfilled the inclusion and exclusion criteria, involving 9,703 subjects. Overall, antiviral therapy for HCV was associated with a reduced risk of all-type complications and surgical complications. Moreover, we found that HCV-infected patients without treatment had substantially higher rates of periprosthetic joint infection at any surgery type and follow-up time point. There was a tendency for favoring a lower pooled revision/reoperation rate and mechanical complication rate in treated patients compared with untreated patients, but the differences failed to reach statistical significance. When limiting analysis to patients receiving preoperative direct acting antiviral-based therapy, untreated patients still had a higher surgical complication rate and joint infection rate.

Conclusion: This meta-analysis demonstrated that antiviral therapy for HCV appears to be associated with a reduced risk of surgical complications in TJA patients, particularly periprosthetic joint infection. Thus, direct-acting antiviral therapy could be recommended for patients diagnosed with HCV.
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http://dx.doi.org/10.1016/j.arth.2022.04.027DOI Listing
April 2022

Identification, functional characterization and expression pattern of myeloid differentiation factor 88 (MyD88) in Nibea albiflora.

Fish Shellfish Immunol 2022 May 25;124:380-390. Epub 2022 Apr 25.

National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, PR China. Electronic address:

Myeloid differentiation factor 88 (MyD88), composed of an N-terminal death domain and a C-terminal Toll/interleukin (IL)-IR homology domain, is a key connector protein in the TLR signal transduction pathway. In this study a novel isoform of MyD88 in Nibea albiflora (named as NaMyD88) was identified and functionally characterized (GenBank accession no. MN384261.1). Its complete cDNA sequence was 1672 bp and contained an open reading frame of 879 bp encoding 292 amino acid residues, which was similar to its teleost fish counterparts in the length. The theoretical molecular mass was 33.63 kDa and the isoelectric point was 5.24. BLASTp analysis suggested that the deduced amino acids sequence of NaMyD88 shared high identity to the known MyD88, for instance, 94.77% identity with Collichthys lucidus. Sequence analysis showed that NaMyD88 protein was consistent with MyD88 protein of other species at three conserved domains, N-terminal DD, short middle domain and C-terminal TIR, and the TIR domain contained three highly conserved motifs: Box1, Box2, and Box3. NaMyD88 and red fluorescent protein (Dsred) were fused and expressed in the cytoplasm of the epithelioma papulosum cyprini (EPC cells). The NaTLR9-TIR-EGFP fusion protein, which was obtained in our previous studies, showed green fluorescence and mainly distributed in the cytoplasm. After co-transfection, NaMyD88-Dsred and NaTLR9-TIR-EGFP obviously overlapped and displayed orange-yellow color. The results showed that the homologous MyD88-Dsred could interact with NaTLR9-TIR-EGFP. Based on this result pcMV-NaMyD88-TIR-Myc plasmids and the pcDNA3.1-NaTLR9-TIR-flag were constructed and co-transfected into 293T cells for the immunoprecipitation test. According to Western blot, the protein eluted by Flag-beads could be detected by anti-Flag-tag antibody and anti-Myc tag antibody respectively, while the protein without NaTLR9-TIR could not be found, which further proved that TLR and MyD88 could interact each other. The prokaryotic plasmid of MyD88-TIR domain was constructed, expressed in BL21 (DE3) and purified by Ni-NAT super flow resin conforming to the expected molecular weight of 27 kDa with the corresponding active sites for its conferring protein-protein interaction functions. Real-time fluorescence quantitative PCR showed that NaMyD88 could be expressed in intestine, stomach, liver, kidney, gill, heart and spleen, with the highest in the kidney, and it was up-regulated after being infected with Polyinosinic:polycytidylic acid - Poly (I:C) and Pseudomonas plecoglossicida, which showed that NaMyD88 was involved in the immune response of N.albiflora. These data afforded a basis for understanding the role of NaMyD88 in the TLR signaling pathway of N.albiflora.
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http://dx.doi.org/10.1016/j.fsi.2022.04.027DOI Listing
May 2022

A Two-Phase Approach to Fusicoccane Synthesis To Uncover a Compound That Reduces Tumourigenesis in Pancreatic Cancer Cells.

Angew Chem Int Ed Engl 2022 05 25;61(19):e202117476. Epub 2022 Feb 25.

College of Chemistry, Nankai University, 94 Weijin Road, Tianjin, 300071, P. R. China.

Alterbrassicicene D (1) and 3(11)-epoxyhypoestenone (2) were synthesised via a two-phase approach featuring concise construction of the 5-8-5 tricyclic intermediate and a tandem base-mediated epoxide opening-transannular oxa-Michael addition cascade to forge the complex skeleton of 2. The route is scalable and we generated 15 g of the tricyclic intermediate in 8 steps from (R)-limonene and 720 mg of the penultimate bioactive intermediate in a protecting-group-free manner. Our synthesis enabled the structural determination of 2 and provided materials for preliminary anticancer evaluation. The penultimate intermediate showed therapeutic potential in terms of its ability to dramatically reduce the tumourigenic potential of PANC-1 pancreatic cancer cells according to a limiting dilution tumour-initiating assay. Our synthetic approach will facilitate unified access to naturally occurring fusicoccanes and their derivatives for anticancer evaluation.
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http://dx.doi.org/10.1002/anie.202117476DOI Listing
May 2022

Sex-Specific Genomic Region Identification and Molecular Sex Marker Development of Rock Bream (Oplegnathus fasciatus).

Mar Biotechnol (NY) 2022 Mar 5;24(1):163-173. Epub 2022 Feb 5.

Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

Rock bream (Oplegnathus fasciatus) is a valuable commercial marine teleost species, which exhibits sexual dimorphism in growth performance. However, the absence of a rapid and cost-effective sex identification method based on sex-specific genetic marker has impeded study on sex determination mechanisms and breeding applications. In the present study, we firstly developed the PCR method for identifying potential sex-specific sequences in Oplegnathus fasciatus with the next-generation sequencing. Sex-specific genomic regions/loci for sex determination were discovered on Chr2 and Chr6 by genome-wide association analysis, sequencing depth, and heterozygosity comparison between females and males. Candidate sex-determining genes (CCDC63, ITR, WNT4) were furtherly detected in transcriptome data of testes and ovaries. Taken together, a male-specific 34-bp deletion on the Chr2 was identified and developed into molecular marker of sex for O. fasciatus. After validation in individuals with known phenotypic sexes, the accuracy was 100%. This study gives an insight into the mechanism of sex determination in O. fasciatus, and the gender marker is crucial both for future genomic research and for development of efficient and sustainable aquaculture practice.
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http://dx.doi.org/10.1007/s10126-022-10095-2DOI Listing
March 2022

Stratification patterns of anammox granular sludge bed: Linking particle size distribution to microbial activity and community.

Environ Res 2022 Jul 1;210:112763. Epub 2022 Feb 1.

Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, China.

Anammox granular sludge processes are an attractive and efficient biotechnology in the field of wastewater treatment. In this study, the stratification patterns of anammox granular sludge bed (GSB) at steady states were illustrated and its relationship to microbial activity and community were systematically investigated under different nitrogen loading rates (NLRs). Morphological observation and quantitive particle size distribution analysis demonstrated that the GSB at low NLR was mainly composed of micro and fine granules with a big difference between bottom and top sludge layers. But at high NLR, the volumetric mean diameter (VMD) of GSB increased with the size distribution width (Span) declined forming a more homogeneous and coarse granules population. The particle size distribution parameters of GSB could be fast characterized by the optical lightness (L*) parameter (r = -0.771, p < 0.01, n = 16) and held a significant correlation with the nitrogen removal rate (NRR) of anammox system (r > 0.9, p < 0.05). The microbial spatial distribution patterns of different sludge layers were further investigated by high-throughput sequencing. The microbial community α-diversity index and microbial abundance matrix proved that the community structure tend to coverage at high NLR. Significant difference of the relative abundance of microbial community was detected under different NLRs. The VMD of GSB held a significant correlation with the relative abundance of AnAOB (r = 0.556, p < 0.01, n = 16) and other common accompanying bacteria (Denitratisoma and Chloroflexi). This study proved that the apparent particle size distribution patterns of GSB could be a potential auxiliary indicator to reflect the microbial activity and community, which can facilitate the innovative process monitor of anammox system based on visual features.
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http://dx.doi.org/10.1016/j.envres.2022.112763DOI Listing
July 2022

Exploring Sexual Dimorphism in the Intestinal Microbiota of the Yellow Drum (, Sciaenidae).

Front Microbiol 2021 5;12:808285. Epub 2022 Jan 5.

Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan, China.

Most of fish species exhibit striking sexual dimorphism, particularly during growth. There are also sexual dimorphisms of internal organs and biological functions, including those of intestinal microbiota, which likely plays a key role in growth. In this study, the growth and intestinal microbiota of the female, male, and all-female (yellow drums) were comprehensively analyzed. The caged culture female and all-female yellow drums showed higher growth rates than males. A further analysis of the intestinal microbiota showed a significant difference in diversity between females and males in the summer, whereas there were no significant differences in the diversity and richness between females and males in the winter. In contrast, a significant difference in richness was observed between all-female and male fish, regardless of the season. Although the main composition of the intestinal microbiota showed no significant sex differences, the community structure of the intestinal microbiota of yellow drums did. Furthermore, the correlations between intestinal microbial communities are likely to be influenced by sex. The ecological processes of the intestinal microbial communities of the yellow drums showed clear sexual dimorphism. Further network analysis revealed that, although the main components of the network in the intestinal microbiota of female, male, and all-female fish were similar, the network structures showed significant sex differences. The negative interactions among microbial species were the dominant relationships in the intestinal ecosystem, and Bacteroidetes, Firmicutes, and Proteobacteria were identified as the functional keystone microbes. In addition, the functional pathways in the intestinal microbiota of yellow drums showed no significant sexual or seasonal differences. Based on the findings of this study, we gain a comprehensive understanding of the interactions between sex, growth, and intestinal microbiota in yellow drums.
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http://dx.doi.org/10.3389/fmicb.2021.808285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767002PMC
January 2022

Electric-Field-Induced Room-Temperature Antiferroelectric-Ferroelectric Phase Transition in van der Waals Layered GeSe.

ACS Nano 2022 Jan 3. Epub 2022 Jan 3.

Key Laboratory of Polar Materials and Devices (MOE) and Department of Electronics, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

Searching van der Waals ferroic materials that can work under ambient conditions is of critical importance for developing ferroic devices at the two-dimensional limit. Here we report the experimental discovery of electric-field-induced reversible antiferroelectric (AFE) to ferroelectric (FE) transition at room temperature in van der Waals layered α-GeSe, employing Raman spectroscopy, transmission electron microscopy, second-harmonic generation, and piezoelectric force microscopy consolidated by first-principles calculations. An orientation-dependent AFE-FE transition provides strong evidence that the in-plane (IP) polarization vector aligns along the armchair rather than zigzag direction in α-GeSe. In addition, temperature-dependent Raman spectra showed that the IP polarization could sustain up to higher than 700 K. Our findings suggest that α-GeSe, which is also a potential ferrovalley material, could be a robust building block for creating artificial 2D multiferroics at room temperature.
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http://dx.doi.org/10.1021/acsnano.1c09183DOI Listing
January 2022

Hardware-Assisted Security Monitoring Unit for Real-Time Ensuring Secure Instruction Execution and Data Processing in Embedded Systems.

Micromachines (Basel) 2021 Nov 26;12(12). Epub 2021 Nov 26.

School of Electronic and Information Engineering, Beihang University, Beijing 100191, China.

The hardware security of embedded systems is raising more and more concerns in numerous safety-critical applications, such as in the automotive, aerospace, avionic, and railway systems. Embedded systems are gaining popularity in these safety-sensitive sectors with high performance, low power, and great reliability, which are ideal control platforms for executing instruction operation and data processing. However, modern embedded systems are still exposing many potential hardware vulnerabilities to malicious attacks, including software-level and hardware-level attacks; these can cause program execution failure and confidential data leakage. For this reason, this paper presents a novel embedded system by integrating a hardware-assisted security monitoring unit (SMU), for achieving a reinforced system-on-chip (SoC) on ensuring program execution and data processing security. This architecture design was implemented and evaluated on a Xilinx Virtex-5 FPGA development board. Based on the evaluation of the SMU hardware implementation in terms of performance overhead, security capability, and resource consumption, the experimental results indicate that the SMU does not lead to a significant speed degradation to processor while executing different benchmarks, and its average performance overhead reduces to 2.18% on typical 8-KB I/D-Caches. Security capability evaluation confirms the monitoring effectiveness of SMU against both instruction and data tampering attacks. Meanwhile, the SoC satisfies a good balance between high-security and resource overhead.
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http://dx.doi.org/10.3390/mi12121450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708534PMC
November 2021

Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes.

Front Genet 2021 29;12:791179. Epub 2021 Nov 29.

Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan, China.

The rock bream () is a typical fish with a unique multiple sex chromosome system. In this study, we investigated the gene expression profiling in the gonads and brains of both males and females using RNA-Seq to identify sex-related genes and pathways. In accordance with the dimorphic expression profiles, combined with Gene ontology and KEGG enrichment analyses, a number of potential genes and pathways associated with sex determination were obtained from transcriptional analysis, especially some sex-biased genes and pathways. Next, we selected 18 candidate genes and analyzed their expression in different tissues and developmental stages. We found that the expression levels of , , , , and were significantly higher in the testis than those in the ovary or other tissues, whereas the expression levels of , , , , and were significantly higher in the ovary than those in the testis. Furthermore, the expression levels of these genes in different developmental stages of gonads also showed sexually dimorphic patterns, suggesting that they might play important roles during gonadal development. These genes are useful markers for investigating sex determination and differentiation in rock bream. The findings of this study can provide insights into the molecular mechanisms of sex determination and differentiation in fish with multiple sex chromosome systems.
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http://dx.doi.org/10.3389/fgene.2021.791179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668390PMC
November 2021

Macrophage-biomimetic porous [email protected] nanocomposites for dual modal immunotherapy against inflammatory osteolysis.

J Nanobiotechnology 2021 Nov 22;19(1):382. Epub 2021 Nov 22.

Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, 200233, China.

Background: Inflammatory osteolysis, a major complication of total joint replacement surgery, can cause prosthesis failure and necessitate revision surgery. Macrophages are key effector immune cells in inflammatory responses, but excessive M1-polarization of dysfunctional macrophages leads to the secretion of proinflammatory cytokines and severe loss of bone tissue. Here, we report the development of macrophage-biomimetic porous SiO-coated ultrasmall Se particles (porous [email protected] nanospheres) to manage inflammatory osteolysis.

Results: Macrophage membrane-coated porous [email protected] nanospheres([email protected]) attenuated lipopolysaccharide (LPS)-induced inflammatory osteolysis via a dual-immunomodulatory effect. As macrophage membrane decoys, these nanoparticles reduced endotoxin levels and neutralized proinflammatory cytokines. Moreover, the release of Se could induce macrophage polarization toward the anti-inflammatory M2-phenotype. These effects were mediated via the inhibition of p65, p38, and extracellular signal-regulated kinase (ERK) signaling. Additionally, the immune environment created by [email protected] reduced the inhibition of osteogenic differentiation caused by proinflammation cytokines, as confirmed through in vitro and in vivo experiments.

Conclusion: Our findings suggest that [email protected] have an immunomodulatory role in LPS-induced inflammation and bone remodeling, which demonstrates that [email protected] are a promising engineered nanoplatform for the treatment of osteolysis occurring after arthroplasty.
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http://dx.doi.org/10.1186/s12951-021-01128-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607681PMC
November 2021

Underlying function regulators of anaerobic granular sludge: Starvation and dormancy.

Sci Total Environ 2022 Feb 16;807(Pt 3):151024. Epub 2021 Oct 16.

Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, China. Electronic address:

Anaerobic granular sludge (AnGS) is the core of anaerobic granular sludge bed system. In this study, the effect and its mechanism of stopping substrate supply on function of AnGS were investigated. The cutoff of exogenous substrate supply triggered AnGS to enter the dormant state. Some methanization microorganisms sporulated. The number and activity of methanization microorganisms based on 16S rDNA and 16S rRNA/16S rDNA ratio declined and stayed at 45.5% and 0.06% (bacteria), 48.7% and 0.39% (archaea) of the initial vegetative value, respectively. The resuming of exogenous substrate supply promoted AnGS to restore the vegetative state. The spores disappeared. The specific methanization activity of AnGS returned to the original level of 35.82 mL-CH/g-VSS·d, but the delay time for gas production (DTGS) was prolonged from 9.54 to 18.04 h (0-132 d). The dormancy of methanization microorganisms was the main cause for the fluctuation of apparent function and the stability of intrinsic function of AnGS under starvation stress. The dormancy stabilized the structure and sustained the methanization community of AnGS via the reduction of EPS (structure binder/energy reserve) consumption.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151024DOI Listing
February 2022

Chromosome-scale assembly and high-density genetic map of the yellow drum, Nibea albiflora.

Sci Data 2021 10 15;8(1):268. Epub 2021 Oct 15.

Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, 518083, China.

The yellow drum (Nibea albiflora) is an economically important sciaenid fish in East Asian countries. In this study, we sequenced and assembled a near-complete gynogenetic yellow drum genome. We generated 45.63 Gb of Illumina short-reads and 80.27 Gb of PacBio long-reads and assembled them into a 628.01-Mb genome with a contig N50 of 4.42 Mb. Twenty-four chromosomes with a scaffold N50 of 26.73 Mb were obtained using the Hi-C analysis. We predicted a set of 27,069 protein-coding genes, of which 1,581 and 2,583 were expanded and contracted gene families, respectively. The most expanded genes were categorised into the protein binding, zinc-ion binding and ATP binding functional pathways. We built a high-density genetic linkage map that spanned 4,300.2 cM with 24 linkage groups and a resolution of 0.69 cM. The high-quality reference genome and annotated profiles that we produced will not only increase our understanding of the genetic architecture of economic traits in the yellow drum, but also help us explore the evolution and unique biological characteristics of sciaenid fishes.
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http://dx.doi.org/10.1038/s41597-021-01045-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521588PMC
October 2021

Molecular insights of a novel fish Toll-like receptor 9 homologue in Nibea albiflora to reveal its function as PRRs.

Fish Shellfish Immunol 2021 Nov 20;118:321-332. Epub 2021 Sep 20.

National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, PR China.

Toll-like receptors (TLRs) are an important class of molecules involved in non-specific immunity, and they are also the bridge connecting between non-specific immunity and specific immunity. As a vital member of TLR family TLR9 can be activated by bacterial DNA and induce the production of inflammatory cytokines. In this study, a full length of TLR9 homologue of 3677 bp in Nibea albiflora (named as NaTLR9, GenBank accession no: MN125017.1) was characterized, and its ORF was 3180 bp encoding 1059 amino acid residues with a calculated molecular weight of 121.334 kDa (pI = 6.29). Several leucine-rich repeated sequences (LRR domain) and conservative TIR domain were found in NaTLR9, which was mainly expressed in dendritic cells and macrophages. The phylogenetic and synteny analysis further revealed high sequence identity of NaTLR9 with its counterparts of other teleost, confirming their correct nomenclature and conservative during evolution as an important pattern recognition receptor. The NaTLR9-TIR-pEGFP-N1 fusion protein showed green fluorescence and mainly distributed in the cytoplasm. After co-transfection of NaTLR9-TIR-pEGFP-N1 and NaMyD88-pDsRED-Monomer-N1, green fluorescence obviously overlapped with red and changed into yellowish-green, which suggested that there might be the interaction between homologous NaTLR9-TIR and MyD88. Based on this result the pCDNA3.1-NaTLR9-TIR-flag and pcMV-NaMyD88-TIR-Myc plasmids were co-transfected into 293T cells for the immunoprecipitation test. According to Western blot, TLR9 and MyD88 protein could interact with each other. Furthermore, NaTLR9 was ubiquitously expressed in all the investigated tissues, most abundantly in head kidney, followed by stomach, spleen, liver and gill, but lower in muscle. The vitro immune stimulation experiments revealed that Pseudomonas plecoglossicida and polyinosinic-polycytidylic acid [Poly (I:C)] induced higher levels of NaTLR9 mRNA expression with the peaks of 9.52 times at 2 h and 39.91 times at 24 h compared with the control group respectively. The functional domains (LRRs and TIR, named NaTLR9-TIR and NaTLR9-LRR respectively) of NaTLR9 were expressed and purified, the recombinant proteins both could bind three kinds of typical aquatic pathogenic bacteria (Vibrio. parahaemolyticus, Vibrio alginolyticus, and Vibrio harveyi), which showed that NaTLR9 could couple to bacteria by its function domains. The aforementioned results indicated that NaTLR9 played a significant role in the defense against pathogenic bacteria infection in innate immune response of sciaenidae fish, which may provide some further understandings of the regulatory mechanisms in the teleostean innate immune system.
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http://dx.doi.org/10.1016/j.fsi.2021.09.021DOI Listing
November 2021

Characterizing omental PET/CT findings for differentiating tuberculous peritonitis from peritoneal carcinomatosis.

Abdom Radiol (NY) 2021 12 21;46(12):5574-5585. Epub 2021 Sep 21.

The First People's Hospital of Yunnan Province, PET/CT Center, 157 Jinbi Road, Xishan District, Kunming, 650100, Yunnan, People's Republic of China.

Purpose: To characterize and investigate PET/CT findings in the omentum in the differentiation of tuberculous peritonitis (TBP) and peritoneal carcinoma (PC).

Methods: Thirty-nine patients with TBP and 113 patients with PC who underwent PET/CT were retrospectively enrolled. The omental uptake intensity, distribution characteristics, contracture, size and boundary of soft-tissue lesions, and CT patterns were reviewed.

Results: Absent and focal FDG uptake in the lesser omentum was more common in the PC patients (P = 0.034 and P = 0.017, respectively), and diffuse FDG uptake in the lesser omentum was more common in the TBP patients (P < 0.001). An apron-like pattern in the greater omentum commonly occurred in the TBP patients (P = 0.004). Micronodules (< 5 mm) were more common in the TBP patients (P < 0.001), and masses (> 3 cm) were more common in the PC patients (P = 0.001). Smudged and nodular patterns occurred more frequently in the TBP patients than in the PC patients (P < 0.001 and P = 0.003, respectively), and the caked pattern occurred more frequently in the PC patients (P < 0.001). There was no significant difference in the FDG uptake intensity and the boundary of soft-tissue lesions between the TBP and PC patients (P = 0.191 and P = 0.061, respectively).

Conclusion: Diffuse FDG uptake, an apron-like pattern, micronodules, and a smudged and nodular pattern might be significant differential features of TBP. Absent and/or focal FDG uptake, mass, and a caked pattern might be significant differential features of PC.
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http://dx.doi.org/10.1007/s00261-021-03286-3DOI Listing
December 2021

Effect of temperature decrease on anammox granular sludge: Shock and adaptation.

Sci Total Environ 2021 Dec 22;798:149242. Epub 2021 Jul 22.

Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, China. Electronic address:

Cryopreservation is one of the effective methods for the preservation of anammox granular sludge (AnGS). However, the effects of cooling pretreatment on AnGS are still unclear. In this study, the effects of temperature decrease on AnGS property were investigated by designing different cooling modes: constant at room temperature 20-25 °C (CK), sharp cooling to 4 °C (S4), -20 °C (S20) and stepwise cooling to 4 °C (A4), -20 °C (A20). The results showed that compared with CK, the cooling modes in S4, S20, A4 and A20 improved the physical preservability of AnGS, slowing down the changes of color, shape and structure; and elevated the preservation rate of functional bacteria Planctomycetes (phylum level) and Candidatus Brocadia (genus level). The preservation rate of live cells in different experimental groups was 48.4 ± 1.8%(CK), 61.1 ± 3.3%(S4), 37.8 ± 0.8%(S20), 81.7 ± 4.8%(A4), 61.9 ± 3.1%(A20), respectively. The Anaerobic Ammonium Oxidation Bacteria (AnAOB) in the stepwise cooling mode (A4 and A20) were found to enter the dormant state and form "dormant zoogloea", while the AnAOB in the sharp cooling mode (S4 and S20) were observed to enter the shock state with a little change. The findings in this work (especially the dormant state of AnAOB) are helpful to understand the effect of temperature decrease on AnGS and to promote the development of AnGS preservation technology.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149242DOI Listing
December 2021

Dimension effect of anammox granule: Potential vs performance.

Sci Total Environ 2021 Nov 24;795:148681. Epub 2021 Jun 24.

Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, China. Electronic address:

Anammox granule is the key support of anammox sludge bed reactor. In this study, the anammox granules from a steady-state reactor were divided into 6 groups to investigate their dimension effects. The results of batch cultivation showed that the anammox granules with VMD (volume surface mean diameter) of 2.17 mm had the maximum SAA (specific anammox activity) of 399.6 ± 37.6 mg-N/(g-VSS·d). The bacterial community analysis demonstrated that Candidatus Kuenenia was the main detectable AnAOB genus in the anammox granules. Q-PCR together with flow cytometry indicated that the total number of viable AnAOB cells ascended with the increasing anammox granular size, suggesting the enhancement of nitrogen removal potential. On the contrary, the mass transfer efficiency descended with the increasing granular size, indicating the restriction of nitrogen removal performance. The maximum SAA was ascribed to the optimal match between nitrogen removal potential and mass transfer efficiency. The results of this study are helpful to comprehend the nitrogen removal capacity of anammox granules and to promote the optimization of anammox process.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148681DOI Listing
November 2021

NUDCD1 knockdown inhibits the proliferation, migration, and invasion of pancreatic cancer via the EMT process.

Aging (Albany NY) 2021 07 29;13(14):18298-18309. Epub 2021 Jul 29.

School of Stomatology, Wenzhou Medical University, Wenzhou 110013, China.

NudC domain containing 1 (NUDCD1) is an oncoprotein frequently activated or upregulated in various human cancers, but its role in pancreatic cancer (PC) remains unknown. Thus, we aimed to determine the function and mechanism of NUDCD1 in PC. We employed Western blot and quantitative real-time polymerase chain reaction to assess NUDCD1 expression in cells and PC tissues. NUDCD1 was knocked down in Patu8988 and PANC-1 cells. We conducted real-time cell analysis, wound healing assay, transwell assay and colony formation assay to evaluate the metastatic and proliferative abilities of PC cells. Western blot was conducted to assess the expression of markers associated with apoptosis and epithelial-mesenchymal transition (EMT). Also, we established a tumor xenograft model to determine the role of NUDCD1 . NUDCD1 was overexpressed in PC tissues and cells. NUDCD1 knockdown suppressed the invasion, migration, and proliferative abilities of the cells and induced PC cell apoptosis. The specific mechanism of NUDCD1 was related to the modulation of the EMT process. Data obtained from experiments revealed that NUDCD1 knockdown inhibited the tumor growth, proliferation, and metastasis by modulating the EMT and inducing the apoptosis of PC cells.
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http://dx.doi.org/10.18632/aging.203276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351729PMC
July 2021

Characterization of the sex differentiation and gonadal development in small yellow croaker (Larimichthys polyactis) and its hybrid (L. polyactis ♀ × L. crocea ♂).

Fish Physiol Biochem 2021 Oct 29;47(5):1467-1476. Epub 2021 Jul 29.

Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan, 316021, China.

Interspecific hybridization has been considered as a possible approach to improve biological traits and has been applied in aquaculture practices. In the present study, artificial hybridization was carried out in the small yellow croaker (SYC; Larimichthys polyactis) ♀ × large yellow croaker (LYC; L. crocea) ♂ by artificial insemination, and the processes of sex differentiation and gonadal development in SYC and its hybrid were investigated under controlled conditions. Histological analysis of SYC larvae showed that migrating primordial germ cells (PGCs) were observed at 4 days post-hatching (dph), a genital ridge was formed on the dorsal side of the peritoneum at 6 dph, and a pair of primary gonads was first observed at 10 dph. Signs of the differentiated ovary and ovarian cavity were observed at 45 dph. However, some presumptive testes showed alterations in morphology, including an increase in the number of oocytes and an enhanced basophilia at 50 dph. These presumptive testes seemed to alter again, and numerous gonial cells were arranged in cyst-like groups with several degenerating oocytes that developed into residual body-like structures during 60-90 dph. Compared with SYC, the hybrid had a lower number of PGCs and showed retarded gonadal development at the early stage. Ovarian differentiation in the hybrid was observed at 50 dph, while testicular differentiation occurred at 60 dph. The presence of vitellogenic oocytes and spermatozoa at 360 dph in the hybrids suggested that hybrid individuals can undergo successful gametogenesis in females and males, respectively. Overall, the present results suggest that morphological sex differentiation occurred at 40 and 50 dph in SYC and its hybrid, respectively, both of which have normal gametogenesis. Moreover, some level of heterosis (hybrid vigor) occurred in the growth of the hybrid (total length and body weight) compared with that in the growth of SCY over time. Gonadal development of the hybrid was also found to be advanced at 360 dph. The present information will contribute to the potential use and management of these fish for aquaculture.
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http://dx.doi.org/10.1007/s10695-021-00975-0DOI Listing
October 2021

Highly Efficient Dehydrogenation of Formic Acid over Binary Palladium-Phosphorous Alloy Nanoclusters on N-Doped Carbon.

Inorg Chem 2021 Jul 1;60(14):10707-10714. Epub 2021 Jul 1.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Highly efficient dehydrogenation of formic acid (FA) at room temperature is a safe and suitable way to obtain hydrogen and promote the development of hydrogen storage application. Herein, the phosphorous-alloyed Pd nanoclusters loading on nitrogen-doped carbon (PdP/NC) were prepared and recognized as the highly active nanocatalysts for the dehydrogenation of FA. The PdP/NCs with controlled sizes and compositions were prepared by an easy self-limiting synthesis in an aqueous solution. The best PdP/NC exhibited a remarkable catalytic activity with a high turnover frequency of ∼3253.0 h than the compared nanocatalysts for the dehydrogenation of FA at room temperature. The catalytic kinetics and durability studies showed that both the alloyed P in Pd crystals and doped N in the carbon support could effectively tailor the electronic states of the Pd surface and further optimize the adsorption energy of FA. Based on the Sabatier principle, the proper adsorption energy accelerated the dehydrogenation reaction and correspondingly enhanced the activity and durability. The work proposed a high-efficiency nanocatalyst for safe hydrogen generation and may be extended to create other similar nanocatalysts with different compositions and nanostructures.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01403DOI Listing
July 2021

Surface Engineering and Controlled Ripening for Seed-Mediated Growth of Au Islands on Au Nanocrystals.

Angew Chem Int Ed Engl 2021 Jul 28;60(31):16958-16964. Epub 2021 Jun 28.

Department Department of Chemistry, University of California, Riverside, CA, 92521, USA.

Engineering the nucleation and growth of plasmonic metals (Ag and Au) on their pre-existing seeds is expected to produce nanostructures with unconventional morphologies and plasmonic properties that may find unique applications in sensing, catalysis, and broadband energy harvesting. Typical seed-mediated growth processes take advantage of the perfect lattice match between the deposited metal and seeds to induce conformal coating, leading to either simple size increases (e.g., Au on Au) or the formation of core-shell structures (e.g., Ag on Au) with limited morphology change. In this work, we show that the introduction of a thin layer of metal with considerable lattice mismatch can effectively induce the nucleation of well-defined Au islands on Au nanocrystal seeds. By controlling the interfacial energy between the seed and the deposited material, the oxidative ripening, and the surface diffusion of metal precursors, we can regulate the number of islands on the seeds and produce complex Au nanostructures with morphologies tunable from core-satellites to tetramers, trimers, and dimers.
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http://dx.doi.org/10.1002/anie.202105856DOI Listing
July 2021

Dynamically Switchable Polarization-Independent Triple-Band Perfect Metamaterial Absorber Using a Phase-Change Material in the Mid-Infrared (MIR) Region.

Micromachines (Basel) 2021 May 11;12(5). Epub 2021 May 11.

Jiangsu Key Laboratory for Optoelectronic Detection of Atmosphere and Ocean, School of Physics and Optoelectronic Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.

A tunable metamaterial absorber (MMA) by reversible phase transitions in a mid-infrared regime is theoretically investigated. The absorber is composed of a molybdenum (Mo)-germanium-antimony-tellurium (GeSbTe, GST)-Mo nanodisk structure superimposed on the GST-AlO (aluminum oxide)-Mo film. Studies have shown that the combination of the inlaid metal-medium dielectric waveguide mode and the resonant cavity mode and the excitation of the propagating surface plasmon mode are the main reasons for the formation of the triple-band high absorption. Additionally, through the reversible phase change, the transition from high absorption to high reflection in the mid-infrared region is realized. The symmetry of the absorber eliminates the polarization dependence, and the near unity absorption efficiency can be maintained by incidence angles up to 60°. The presented method will enhance the functionality of the absorber and has the potential for the applications that require active control over light absorption.
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http://dx.doi.org/10.3390/mi12050548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151617PMC
May 2021

High-Efficiency Parallel Cryptographic Accelerator for Real-Time Guaranteeing Dynamic Data Security in Embedded Systems.

Micromachines (Basel) 2021 May 15;12(5). Epub 2021 May 15.

School of Electronic and Information Engineering, Beihang University, Beijing 100191, China.

Dynamic data security in embedded systems is raising more and more concerns in numerous safety-critical applications. In particular, the data exchanges in embedded Systems-on-Chip (SoCs) using main memory are exposing many security vulnerabilities to external attacks, which will cause confidential information leakages and program execution failures for SoCs at key points. Therefore, this paper presents a security SoC architecture with integrating a four-parallel Advanced Encryption Standard-Galois/Counter Mode (AES-GCM) cryptographic accelerator for achieving high-efficiency data processing to guarantee data exchange security between the SoC and main memory against bus monitoring, off-line analysis, and data tampering attacks. The architecture design has been implemented and verified on a Xilinx Virtex-5 Field Programmable Gate Array (FPGA) platform. Based on evaluation of the cryptographic accelerator in terms of performance overhead, security capability, processing efficiency, and resource consumption, experimental results show that the parallel cryptographic accelerator does not incur significant performance overhead on providing confidentiality and integrity protections for exchanged data; its average performance overhead reduces to as low as 2.65% on typical 8-KB I/D-Caches, and its data processing efficiency is around 3 times that of the pipelined AES-GCM construction. The reinforced SoC under the data tampering attacks and benchmark tests confirms the effectiveness against external physical attacks and satisfies a good trade-off between high-efficiency and hardware overhead.
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http://dx.doi.org/10.3390/mi12050560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155854PMC
May 2021

Potential of anammox process towards high-efficient nitrogen removal in wastewater treatment: Theoretical analysis and practical case with a SBR.

Chemosphere 2021 Oct 30;281:130729. Epub 2021 Apr 30.

Department of Environmental Engineering, Zhejiang University, Hangzhou, China. Electronic address:

Anammox process has been widely applied in the wastewater nitrogen removal for its high rate and low cost. However, few researches reported the process potential in treating low-strength nitrogen wastewater. In this study, a sequencing batch reactor (SBR) was taken to explore the feasibility of low-strength nitrogen wastewater treatment by anammox process in theory and practice. The practical operation indicated that the effluent with satisfactory quality (1.90 ± 0.70 mg-TN·L) could be achieved, when the SBR was fed with low-strength nitrogen influent (6.20 ± 0.45 mg-NH-N·L and 7.96 ± 0.59 mg-NO-N·L). The hydraulic retention time (HRT), nitrogen removal efficiency, nitrogen removal rate (NRR) and hydraulic loading rate of SBR were 5.42 h, 86.5%, 0.054 kg-N·m·d and 4.43 m·m·d during the 79-day operation, respectively. The theoretical analysis revealed the potential of anammox SBR. When SBR is stably operated, the maximum NRR would be 0.062 kg-N·m·d if the effluent nitrogen was required to be as low as 3 mg·L. The NRR value is feasible for engineering. However, considering the lower specific substrates utilization rate in practice, the maximum stable NRR was calibrated and found inefficient afterwards. In order to improve the potential of anammox process, the reactors without back mixing and with periodic bioaugmentation should be taken in priority for the engineering applications. In particular, the bioaugmentation frequency and single addition amount were calculated as 7 d and 0.3 g-VSS·L, respectively. The results may provide guidance for the development of high-efficient and stable nitrogen removal process under low-strength condition.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130729DOI Listing
October 2021

Rapid Aqueous Synthesis of Large-Size and Edge/Defect-Rich Porous Pd and Pd-Alloyed Nanomesh for Electrocatalytic Ethanol Oxidation.

Chemistry 2021 Aug 17;27(43):11175-11182. Epub 2021 Jun 17.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, Jiangsu, 210023, P.R. China.

In this work, a facile aqueous synthesis strategy was used (complete in 5 min at room temperature) to produce large-size Pd, PdCu, and PdPtCu nanomeshes without additional organic ligands or solvent and the volume restriction of reaction solution. The obtained metallic nanomeshes possess graphene-like morphology and a large size of dozens of microns. Abundant edges (coordinatively unsaturated sites, steps, and corners), defects (twins), and mesopores are seen in the metallic ultrathin structures. The formation mechanism for porous Pd nanomeshes disclosed that they undergo oriented attachment growth along the ⟨111⟩ direction. Owing to structural and compositional advantages, PdCu porous nanomeshes with certain elemental ratios (e. g., Pd Cu ) presented enhanced electrocatalytic performance (larger mass activity, better CO tolerance and stability) toward ethanol oxidation.
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http://dx.doi.org/10.1002/chem.202101144DOI Listing
August 2021

Total synthesis of (-)-brazilane via a lipase-catalyzed desymmetrisation reaction.

Nat Prod Res 2021 May 10:1-9. Epub 2021 May 10.

Laboratory Animal Center, Shanxi Cancer Institute, Taiyuan, China.

Herein, we described the asymmetric total synthesis of (-)-brazilane, an optically active natural product. The key steps of this synthetic approach are a lipase-catalyzed desymmetrisation reaction of a prochiral diol using vinyl acetate to prepare a chiral primary alcohol and a trifluoroacetic acid-catalyzed one pot intramolecular tandem Prins/Friedel-Crafts reaction used to construct the -fused chromane and indane framework.
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http://dx.doi.org/10.1080/14786419.2021.1922403DOI Listing
May 2021

Ultrathin and Wavy PdB Alloy Nanowires with Controlled Surface Defects for Enhanced Ethanol Oxidation Electrocatalysis.

ACS Appl Mater Interfaces 2021 Apr 12;13(15):17599-17607. Epub 2021 Apr 12.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Engineering crystalline structures/defects and elemental compositions is synthetically critical to optimize surface features of noble metal nanocrystals and thus improve their catalytic performances in various reactions. In this manuscript, we report a facile one-step aqueous synthesis of one-dimensional (1D) noble metal-metalloid alloy nanowires (NWs) with an ultrathin and wavy morphology, controlled crystalline defects, and binary PdB compositions as a highly efficient catalyst toward the electrochemical ethanol oxidation reaction (EOR). We show that the utilization of hexadecylpyridinium chloride as functional surfactant is of great importance to confine in-the-columnar epitaxial nucleation of anisotropic ultrathin PdB NWs, while the attachment growth precisely controls their surface crystalline defects with a wavy morphology. Meanwhile, this strategy is synthetically universal and can be readily extended to engineer an ultrathin wavy morphology and crystalline defect of ternary PdMB (M = Cu and Pt) alloy NWs. Owing to multiple structural and compositional merits, resultant PdB alloy NWs synergistically expose more electrocatalytically active sites and also kinetically accelerate the removal of CO-related poisons, remarkably improving electrocatalytic EOR activity and stability compared to their counterpart catalysts. Besides, wavy PdB alloy NWs are also electrochemically more active for electrocatalytic oxidation of other alcohols (methanol, glycerol, and glucose). The findings reported here thus shed a bright light on rational design of the high-performance metal alloy catalysts for their potential applications in fine chemical synthesis, fuel cells, and beyond.
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http://dx.doi.org/10.1021/acsami.1c02039DOI Listing
April 2021

SIRT6‑specific inhibitor OSS‑128167 exacerbates diabetic cardiomyopathy by aggravating inflammation and oxidative stress.

Mol Med Rep 2021 05 24;23(5). Epub 2021 Mar 24.

Department of Pain Medicine, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.

Diabetic cardiomyopathy (DCM) is a serious complication of diabetes, which importantly contributes to the increased mortality of patients with diabetes. The development of DCM is accompanied by numerous pathological mechanisms, including oxidative stress and chronic inflammation. Accordingly, the present study aimed to determine the effects of the sirtuin 6 (SIRT6) inhibitor OSS‑128167 on DCM using a mouse model of streptozotocin (STZ)‑induced diabetes and high glucose (HG)‑treated cardiomyocytes. C57BL/6 mice were intraperitoneally injected with STZ for 5 days to simulate the diabetic cardiomyopathy model. Mice with STZ‑induced diabetes (STZ‑DM1) were orally administered OSS‑128167 (20 or 50 mg/kg) through gavage every other day. The expression of SIRT6 in myocardial tissue was detected using western blotting. Tissue staining (hematoxylin and eosin and Masson's trichrome) was used to characterize myocardial structure, TUNEL fluorescent staining was used to detect myocardial apoptosis, and immunohistochemical staining was used to detect the expression of inflammatory factors in myocardial tissue. Dihydroethidium staining and a malondialdehyde (MDA) detection kit were used to detect the oxidative stress levels in myocardial tissues. , H9c2 cells were pre‑incubated with OSS‑128167 for 1 h and then stimulated with HG (33 mM) for various durations. Expression levels of fibrosis markers, collagen‑1 and transforming growth factor (TGF)‑β, apoptosis‑related proteins, Bax, Bcl‑2 and cleaved‑poly ADP‑ribose polymerase, tumor necrosis factor‑α and the oxidative stress metabolite, 3‑nitrotyrosine were analyzed using western blotting and reverse transcription‑quantitative PCR. Commercially available kits were used to detect the activity of caspase‑3 and the content of MDA in the H9c2 cell line. The corresponding results demonstrated that OSS‑128167 aggravated diabetes‑induced cardiomyocyte apoptosis and fibrosis in mice. Mechanistically, OSS‑128167 was revealed to increase the levels of inflammatory factors and reactive oxygen species (ROS) and . In conclusion, OSS‑128167 facilitated the inflammatory response and promoted the production of ROS while aggravating DCM development. These findings indicated that SIRT6 may target two closely combined and interacting pathological processes, the inflammatory response and oxidative stress, and may serve as a potentially advantageous therapeutic target.
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http://dx.doi.org/10.3892/mmr.2021.12006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986000PMC
May 2021

Anaerobic condition induces a viable but nonculturable state of the PCB-degrading Bacteria Rhodococcus biphenylivorans TG9.

Sci Total Environ 2021 Apr 9;764:142849. Epub 2020 Oct 9.

Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China. Electronic address:

Significant microbial removal of highly chlorinated polychlorinated biphenyls (PCBs) requires the cooperation of anaerobic and aerobic bacteria. During the sequencing process of anaerobic dechlorination and aerobic degradation of PCBs, aerobic degrading bacteria have to undergo anaerobic stress. However, the survival strategy of aerobic degrading bacteria under anaerobic condition is not well-understood. In this study, the culturable cells of Rhodococcus biphenylivorans TG9 decreased from 10 CFU/mL to values below the detection limit after 60 days of anaerobic stress while the viable cells remained 10-10 cells/mL, indicating that anaerobic condition induced TG9 entering into the viable but nonculturable (VBNC) state. Cell resuscitation was observed when oxygen was supplied further confirming the VBNC state of TG9. The results of single-cell Raman spectroscopy combined with heavy water indicated the significant decrease of metabolic activity after TG9 entering into the VBNC state. Additionally, the degradation ability of TG9 in the VBNC state was also significantly reduced, while it recovered after resuscitation. Our research proved that entering into the VBNC state is a survival strategy of TG9 under anaerobic conditions, and the limited culturability and degrading capacity could be overcome by resuscitation. The present study provides new insights for improving the remediation efficiency of PCBs contamination.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142849DOI Listing
April 2021

Analysis and verification of fluorescence super-resolution microscopy via polarization modulation in reciprocal space.

J Opt Soc Am A Opt Image Sci Vis 2021 Mar;38(3):337-343

Based on the polarization property of fluorescent dipoles, fluorescence super-resolution microscopy recently has been proposed by modulating the polarization of the excitation light. In this technique, the super-resolution image is reconstructed by processing the polarization-modulated fluorescence image stack with an iteration algorithm. However, the mechanism of resolution improvement by polarization modulation has been questioned. In this paper, the mechanism of resolution enhancement by polarization modulation is analyzed in reciprocal space. The mathematical model and the reconstruction algorithm of fluorescence super-resolution microscopy via polarization modulation are proposed in reciprocal space. The corresponding simulation results and analysis show that polarization modulation can enlarge the highest detected spatial frequency of fluorescence microscopy to achieve super resolution, which verifies the role of polarization modulation in resolution improvement and provides a useful reference to study fluorescence super-resolution microscopy via polarization modulation in reciprocal space.
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http://dx.doi.org/10.1364/JOSAA.406029DOI Listing
March 2021
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