Publications by authors named "Dongdong Li"

375 Publications

A siRNA-Assisted Assembly Strategy to Simultaneously Suppress "Self" and Upregulate "Eat-Me" Signals for Nanoenabled Chemo-Immunotherapy.

ACS Nano 2021 Sep 20. Epub 2021 Sep 20.

School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, People's Republic of China.

Effectively activating macrophages that can engulf cancer cells is a promising immunotherapeutic strategy but remains a major challenge due to the expression of "self" signals (e.g., CD47 molecules) by tumor cells to prevent phagocytosis. Herein, we explored a siRNA-assisted assembly strategy for the simultaneous delivery of siRNA and mitoxantrone hydrochloride (MTO·2HCl) via PLGA-based nanoparticles. The siRNA suppressed a "self" signal by silencing the CD47 gene, while the MTO induced surface exposure of calreticulin (CRT) to provide an "eat-me" signal. The siRNA-assisted assembly strategy synergistically increased the phagocytosis of tumor cells by macrophages, promoted effective antigen presentation, and initiated T cell-mediated immune responses in two aggressive tumor animal models of melanoma and colon cancer, eventually achieving significantly improved antitumor activity. This study provides a straightforward codelivery strategy to simultaneously suppress "self" and upregulate "eat-me" signals to potentiate macrophage-mediated immunotherapy.
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http://dx.doi.org/10.1021/acsnano.1c04458DOI Listing
September 2021

[Establishment of chimeric antigen receptor NK92MI cells recognizing HBsAg].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):788-793

Department of Infection Diseases, First Affiliated Hospital, Bengbu Medical College, Core Partner of National Clinical Research Center for Infection Diseases, Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu 233030, China. *Corresponding author, E-mail:

Objective To construct a lentiviral expression vector of chimeric antigen receptor (CAR) recognizing hepatitis B surface antigen (HBsAg) and make it expressed in NK92MI cells. Methods The CAR was designed and synthesized with the sequence of single chain variable fragment (scFv). Next it was cloned into a lentivirus expression vector, followed by packaging, concentration and titer determination. Then HEK293T cells were transduced with the concentrated lentivirus. The expression of CD3 zeta chain and the infection efficiency in the NK92MI cells were detected by Western blotting. Results The lentiviral expression vector of CAR for HBsAg recognition was successfully constructed, and the titer was ≥10 transducing units (TU)/mL. The expression of protein CD3zeta was verified in the NK92MI cells after infected with the lentiviral vector. Conclusion CAR-NK92MI cells recognizing HBsAg has been established successfully.
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September 2021

Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis.

Bioengineered 2021 12;12(1):7165-7177

School of Life Science, Bengbu Medical College, Bengbu, China.

Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of miR-30 c in HCC was comprehensively investigated by using bioinformatics and experiments . The multiple databases were combined to predict and screen the target genes and upstream lncRNAs of miR-30 c, and then constructed a competitive endogenous RNA (ceRNA) regulatory network with miR-30 c as the central miRNA. The miR-30 c-related ceRNA regulatory network was also initially validated . The results showed that miR-30 c over-expression could inhibit proliferation, migration, invasion, induce apoptosis, and increase G0/G1 phase ratio of HCC cells. Three miR-30 c upstream lncRNAs and 12 miR-30 c target genes were expressed in HCC cells with increased expression and poor prognosis, and a miR-30 C-related ceRNA regulatory network was constructed. This study verified miR-30 c as an inhibitory factor in the progression of HCC and performed analyses on the miR-30 c regulatory network, which might provide potential target information for HCC prognoses and therapies. However, further experiments and studies including clinical trials will be conducted to validate our results.
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http://dx.doi.org/10.1080/21655979.2021.1979439DOI Listing
December 2021

Genetic Dissection of Phosphorus Use Efficiency in a Maize Association Population under Two P Levels in the Field.

Int J Mol Sci 2021 Aug 27;22(17). Epub 2021 Aug 27.

Key Laboratory of Crop Heterosis and Utilization, the Ministry of Education, Key Laboratory of Crop Genetic Improvement, Beijing Municipality, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

Phosphorus (P) deficiency is an important challenge the world faces while having to increase crop yields. It is therefore necessary to select maize ( L.) genotypes with high phosphorus use efficiency (PUE). Here, we extensively analyzed the biomass, grain yield, and PUE-related traits of 359 maize inbred lines grown under both low-P and normal-P conditions. A significant decrease in grain yield per plant and biomass, an increase in PUE under low-P condition, as well as significant correlations between the two treatments were observed. In a genome-wide association study, 49, 53, and 48 candidate genes were identified for eleven traits under low-P, normal-P conditions, and in low-P tolerance index (phenotype under low-P divided by phenotype under normal-P condition) datasets, respectively. Several gene ontology pathways were enriched for the genes identified under low-P condition. In addition, seven key genes related to phosphate transporter or stress response were molecularly characterized. Further analyses uncovered the favorable haplotype for several core genes, which is less prevalent in modern lines but often enriched in a specific subpopulation. Collectively, our research provides progress in the genetic dissection and molecular characterization of PUE in maize.
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http://dx.doi.org/10.3390/ijms22179311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430673PMC
August 2021

Plasticity and domestication of root anatomy in maize-teosinte derived population.

J Exp Bot 2021 Sep 6. Epub 2021 Sep 6.

College of Resources and Environmental Sciences, National Academy of Agriculture Green Development, Key Laboratory of Plant-Soil Interactions of MOE, China Agricultural University, Beijing, 100193, China.

Maize (Zea mays L.) underwent profound changes during domestication in root anatomy for environmental adaptation. However, the genetic mechanism of maize root anatomy domestication and plasticity remains unclear. In this study, a high-resolution mapping was performed for nine root anatomical traits using a maize-teosinte population (mexicana × Mo17) across three environments. Large genetic variations were detected for different root anatomical traits. The cortex area, stele area, aerenchyma area, xylem vessels number and cortical cell number had large variations across three environments, indicating high plasticity. Sixteen quantitative trait loci (QTL) were identified, including seven QTL with QTL × Environment interaction (EIQTL) for high plastic traits and nine QTL without QTL × Environment interaction (SQTL). Most of the root loci were consistent with shoot QTL depicting domestication signals. Combining transcriptome and genome-wide association study (GWAS) revealed that ZmPILS4 serve as a candidate gene underlying a major QTL of xylem traits. The near-isogenic lines (NILs) with lower expression of ZmPILS4 had 18-24% more IAA concentration in the root tip and 8-15% more xylem vessels. Significant domestication signal in promoter region suggested that ZmPILS4 was involved in maize domestication and adaptation. These results divulged the potential genetic basis of root anatomy plasticity and domestication.
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http://dx.doi.org/10.1093/jxb/erab406DOI Listing
September 2021

Application of silver nanotriangles as a novel contrast agent in tumor computed tomography imaging.

Nanotechnology 2021 Sep 15;32(49). Epub 2021 Sep 15.

School of Medicine, Southeast University, Nanjing, Jiangsu, People's Republic of China.

This study aimed to prepare chitosan-coated silver nanotriangles (AgNTs) and assess their computed tomography (CT) contrast property byandexperiments. AgNTs with a range of sizes were synthesized by a seed-based growth method, and subsequently characterized by transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy and dynamic light scattering. The x-ray attenuation capability of all prepared AgNTs was evaluated using micro CT. The CT contrast effect of AgNTs with the highest x-ray attenuation coefficient was investigated in MDA-MB-231 breast cancer cells and a mouse model of breast cancer. The TEM results displayed that all synthesized AgNTs were triangular in shape and their mean edge lengths ranged from 60 to 149 nm. All AgNTs tested exhibited stronger x-ray attenuation capability than iohexol at the same mass concentration of the active elements, and the larger the AgNTs size, the higher the x-ray attenuation coefficient. AgNTs with the largest size were selected for further research, due to their strongest x-ray attenuation capability and best biocompatibility. The attenuation coefficient of breast cancer cells treated with AgNTs increased in a particle concentration-dependent manner.CT imaging showed that the contrast of the tumor injected with AgNTs was significantly enhanced. These findings indicated that AgNTs could be a promising candidate for highly efficient tumor CT contrast agents.
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http://dx.doi.org/10.1088/1361-6528/ac21efDOI Listing
September 2021

RNA editing restricts hyperactive ciliary kinases.

Science 2021 08;373(6558):984-991

Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.

Protein kinase activity must be precisely regulated, but how a cell governs hyperactive kinases remains unclear. In this study, we generated a constitutively active mitogen-activated protein kinase DYF-5 (DYF-5CA) in that disrupted sensory cilia. Genetic suppressor screens identified that mutations of ADR-2, an RNA adenosine deaminase, rescued ciliary phenotypes of We found that animals abnormally transcribed antisense RNAs that pair with messenger RNA (mRNA) to form double-stranded RNA, recruiting ADR-2 to edit the region ectopically. RNA editing impaired mRNA splicing, and the resultant intron retentions blocked DYF-5CA protein translation and activated nonsense-mediated mRNA decay. The kinase RNA editing requires kinase hyperactivity. The similar RNA editing-dependent feedback regulation restricted the other ciliary kinases NEKL-4/NEK10 and DYF-18/CCRK, which suggests a widespread mechanism that underlies kinase regulation.
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http://dx.doi.org/10.1126/science.abd8971DOI Listing
August 2021

Reactive oxygen species induced by uric acid promote NRK‑52E cell apoptosis through the NEK7‑NLRP3 signaling pathway.

Mol Med Rep 2021 Oct 20;24(4). Epub 2021 Aug 20.

Department of Nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, TCM Institute of Kidney Disease, Shanghai University of Traditional Chinese Medicine, Key Laboratory of Liver and Kidney Diseases (Shanghai University of Traditional Chinese Medicine), Ministry of Education, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, P.R. China.

Increasing uric acid (UA) could induce renal tubular epithelial cell (NRK‑52E) injury. However, the specific mechanism by which UA induces renal tubular epithelial cell injury remains unknown. It was hypothesized that UA induces renal tubular epithelial cell injury through reactive oxygen species (ROS) and the Never in mitosis gene A (NIMA)‑related kinase 7 (NEK7)/NLR family pyrin domain containing 3 (NLRP3) signaling pathway. TUNEL assay and flow cytometry were applied to measure apoptosis, and the results of the present study showed that UA treatment induced apoptosis of NRK‑52E cells in a concentration‑dependent manner. Western blotting was performed to determine the expression levels of cleaved caspase‑3, Bax and Bcl‑xl, it was found that levels were significantly increased after UA treatment in NRK‑52E cells. ROS and apoptosis were predominantly induced in NRK‑52E cells and there was an association between ROS and apoptosis. Enhanced expression of NEK7, NLRP3, apoptosis‑associated speck‑like and caspase‑1 were observed in NRK‑52E cells treated with UA. The ROS inhibitor, N‑acetyl‑l‑cysteine, exerted a protective effect on the UA‑induced apoptosis of tubular epithelial cells by reducing excess ROS production, which significantly inhibited NEK7 and NLRP3 inflammasome activation. These results indicated that UA activates ROS and induces apoptosis of NRK‑52E cells. The mechanism might be related to the regulation of the NEK7/NLRP3 signaling pathway.
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http://dx.doi.org/10.3892/mmr.2021.12368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383041PMC
October 2021

EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm ().

Front Plant Sci 2021 26;12:722596. Epub 2021 Jul 26.

College of Tropical Crops, Hainan University, Haikou, China.

, one of the MADS-box transcription factors in oil palm, has a high expression level in the late fruit development of the oil palm fruit mesocarp. At the same time, it is also predicted to be the target gene of which has been identified in previous research. In this paper, we focused on the function and regulatory mechanism of the gene in oil palm lipid metabolism. The results indicated that the transcription level of was highest in the fourth stage, and a dual-luciferase reporter assay proved that the expression level was downregulated by . In both the OX seeds and oil palm embryonic calli, the total lipid contents were significantly decreased, but the contents of C18:0 and C18:3 in OX lines were significantly increased. As expected, EgmiR5179 weakened the inhibitory effect of on the oil contents in transgenic plants that coexpressed and (OXEgmiR5179-). Moreover, yeast two-hybrid and BiFC analyses suggested that there was an interaction between the protein and EgGLO1 protein, which had been proven to be capable of regulating fatty acid synthesis in our previous research work. In summary, a model of the molecular mechanism by which miRNA5179 targets to regulate oil biosynthesis was hypothesized, and the research results provide new insight into lipid accumulation and molecular regulation in oil palm.
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http://dx.doi.org/10.3389/fpls.2021.722596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350733PMC
July 2021

Preoperative Prediction of Lymphovascular Space Invasion in Cervical Cancer With Radiomics -Based Nomogram.

Front Oncol 2021 12;11:637794. Epub 2021 Jul 12.

Department of Radiotherapy, Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

Purpose: To build and evaluate a radiomics-based nomogram that improves the predictive performance of the LVSI in cervical cancer non-invasively before the operation.

Method: This study involved 149 patients who underwent surgery with cervical cancer from February 2017 to October 2019. Radiomics features were extracted from T2 weighted imaging (T2WI). The radiomic features were selected by logistic regression with the least absolute shrinkage and selection operator (LASSO) penalty in the training cohort. Based on the selected features, support vector machine (SVM) algorithm was used to build the radiomics signature on the training cohort. Incorporating radiomics signature and clinical risk factors, the radiomics-based nomogram was developed. The sensitivity, specificity, accuracy, and area under the curve (AUC) and Receiver operating characteristic (ROC) curve were calculated to assess these models.

Result: The radiomics model performed much better than the clinical model in both training (AUCs 0.925 0.786, accuracies 87.5% 70.5%, sensitivities 83.6% 41.7% and specificities 90.9% 94.7%) and testing (AUCs 0.911 0.706, accuracies 84.0% 71.3%, sensitivities 81.1% 43.4% and specificities 86.4% 95.0%). The combined model based on the radiomics signature and tumor stage, tumor infiltration depth and tumor pathology yielded the best performance (training cohort, AUC = 0.943, accuracies 89.5%, sensitivities 85.4% and specificities 92.9%; testing cohort, AUC = 0.923, accuracies 84.6%, sensitivities 84.0% and specificities 85.1%).

Conclusion: Radiomics-based nomogram was a useful tool for predicting LVSI of cervical cancer. This would aid the selection of the optimal therapeutic strategy and clinical decision-making for individuals.
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http://dx.doi.org/10.3389/fonc.2021.637794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311659PMC
July 2021

Child maltreatment and protective assets in the development of internalising and externalising problems: A study of youth offenders.

J Adolesc 2021 Aug 21;91:35-47. Epub 2021 Jul 21.

Probation and Community Rehabilitation Service, Ministry of Social and Family Development, Singapore. Electronic address:

Introduction: An emerging trend in child maltreatment research focuses on identifying protective assets that contribute to youth resilience. Extending the trend, this study examines not just whether but also how protective assets in various domains (e.g., the individual, family, and schools) are associated with internalising and externalising problems among youth offenders, which is a population that typically reports a high prevalence of child maltreatment experiences.

Methods: This study used the baseline data of 790 youth offenders (85% male) aged between 12 and 19 years old from the EPYC project, a national longitudinal study in Singapore. Structural equation modelling was conducted to test the direct effects and interactive effects of protective assets and child maltreatment on internalising and externalising problems.

Results: For direct effects, maltreated youth offenders reported higher levels of internalising and externalising problems than their non-maltreated counterparts. Higher levels of peer assets were directly related to lower levels of externalising, but not internalising problems. For interactive effects, overall protective assets, school/work assets and internal assets had significant buffering effects against physical/emotional abuse on externalising problems, whereas peer assets showed significant buffering effects against sexual abuse on internalising problems.

Conclusion: An overall level of protective assets, as well as assets from specific domains (peer, school/work, and internal assets) could provide protective effects on problematic behaviours among youth offenders. Interventions seeking to address youth internalising and externalising problems should focus on reducing child maltreatment incidence and enhancing protective assets within these domains.
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http://dx.doi.org/10.1016/j.adolescence.2021.07.002DOI Listing
August 2021

Ti C T MXene Conductive Layers Supported Bio-Derived Fe Se /MXene/Carbonaceous Nanoribbons for High-Performance Half/Full Sodium-Ion and Potassium-Ion Batteries.

Adv Mater 2021 Aug 19;33(34):e2101535. Epub 2021 Jul 19.

Sino-Russian International Joint Laboratory for Clean Energy and Energy Conversion Technology, College of Physics, International Center of Future Science, Jilin University, Changchun, 130012, P. R. China.

Owing to their cost-effectiveness and high energy density, sodium-ion batteries (SIBs) and potassium-ion batteries (PIBs) are becoming the leading candidates for the next-generation energy-storage devices replacing lithium-ion batteries. In this work, a novel Fe Se heterostructure is prepared on fungus-derived carbon matrix encapsulated by 2D Ti C T MXene highly conductive layers, which exhibits high specific sodium ion (Na ) and potassium ion (K ) storage capacities of 610.9 and 449.3 mAh g at a current density of 0.1 A g , respectively, and excellent capacity retention at high charge-discharge rates. MXene acts as conductive layers to prevent the restacking and aggregation of Fe Se sheets on fungus-derived carbonaceous nanoribbons, while the natural fungus functions as natural nitrogen/carbon source to provide bionic nanofiber network structural skeleton, providing additional accessible pathways for the high-rate ion transport and satisfying surface-driven contribution ratios at high sweep rates for both Na/K ions storages. In addition, in situ synchrotron diffraction and ex situ X-ray photoelectron spectroscopy measurements are performed to reveal the mechanisms of storage and de-/alloying conversion process of Na in the Fe Se /MXene/carbonaceous nanoribbon heterostructure. As a result, the assembled Na/K full cells containing MXene-supported Fe Se @carbonaceous anodes possess stable large-ion storage capabilities.
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http://dx.doi.org/10.1002/adma.202101535DOI Listing
August 2021

Atrial fibrillation in critically ill patients who received prolonged mechanical ventilation: a nationwide inpatient report.

Korean J Intern Med 2021 Jul 12. Epub 2021 Jul 12.

Department of Health Statistics, Second Military Medical University, Shanghai, China.

Background/aims: To evaluate temporal trends of atrial fibrillation (AF) prevalence in critically ill patients who received prolonged mechanical ventilation (MV) in the United States.

Methods: We used the 2008 to 2014 National Inpatient Sample to compute the weighted prevalence of AF among hospitalized adult patients on prolonged MV. We used multivariable-adjusted models to evaluate the association of AF with clinical factors, in-hospital mortality, hospitalization cost, and length of stay (LOS).

Results: We identified 2,578,165 patients who received prolonged MV (21.27% of AF patients). The prevalence of AF increased from 14.63% in 2008 to 24.43% in 2014 (p for trend < 0.0001). Amongst different phenotypes of critically ill patients, the prevalence of AF increased in patients with severe sepsis, asthma exacerbation, congestive heart failure exacerbation, acute stroke, and cardiac arrest. Older age, male sex, white race, medicare access, higher income, urban teaching hospital setting, and Western region were associated with a higher prevalence of AF. AF in critical illness was a risk factor for in-hospital death (odds ratio, 1.13; 95% confidence interval, 1.11 to 1.15), but in-hospital mortality in critically ill patients with AF decreased from 11.6% to 8.3%. AF was linked to prolonged LOS (2%, p < 0.0001) and high hospitalization cost (4%, p < 0.0001). LOS (-1%, p < 0.0001) and hospitalization cost (-4%, p < 0.0001) decreased yearly.

Conclusions: The prevalence of comorbid AF is increasing, particularly in older patients. AF may lead to poorer prognosis, and high-quality intensive care is imperative for this population.
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http://dx.doi.org/10.3904/kjim.2020.142DOI Listing
July 2021

Discovery of a potent MLL1 and WDR5 protein-protein interaction inhibitor with in vivo antitumor activity.

Eur J Med Chem 2021 Nov 28;223:113677. Epub 2021 Jun 28.

Jiangsu Key Laboratory of Drug Design and Optimization and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

MLL1-WDR5 interaction is essential for the formation of MLL core complex and its H3K4 methyltransferase activity. Disrupting MLL1-WDR5 interaction has been proposed as a potential therapeutic approach in the treatment of leukemia. A "toolkit" of well-characterized chemical probe will allow exploring animal studies. Based on a specific MLL1-WDR5 PPI inhibitor (DDO-2117), which was previously reported by our group, we conducted a bioisosterism approach by click chemistry to discover novel phenyltriazole scaffold MLL1-WDR5 interaction blockers. Here, our efforts resulted in the best inhibitor 24 (DDO-2093) with high binding affinity (K = 11.6 nM) and with improved drug-like properties. Both in vitro and in vivo assays revealed 24 could efficiently block the MLL1-WDR5 interaction. Furthermore, 24 significantly suppressed tumor growth in the MV4-11 xenograft mouse model and showed a favorable safety profile. We propose 24 as a chemical probe that is suitable for in vivo pharmacodynamic and biological studies of MLL1-WDR5 interaction.
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http://dx.doi.org/10.1016/j.ejmech.2021.113677DOI Listing
November 2021

Genomic Prediction across Structured Hybrid Populations and Environments in Maize.

Plants (Basel) 2021 Jun 9;10(6). Epub 2021 Jun 9.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Genomic prediction (GP) across different populations and environments should be enhanced to increase the efficiency of crop breeding. In this study, four populations were constructed and genotyped with DNA chips containing 55,000 SNPs. These populations were testcrossed to a common tester, generating four hybrid populations. Yields of the four hybrid populations were evaluated in three environments. We demonstrated by using real data that the prediction accuracies of GP across structured hybrid populations were lower than those of within-population GP. Including relatives of the validation population in the training population could increase the prediction accuracies of GP across structured hybrid populations drastically. G × E models (including main and genotype-by-environment effect) had better performance than single environment (within environment) and across environment (including only main effect) GP models in the structured hybrid population, especially in the environment where yields had higher heritability. GP by implementing G × E models in two cross-validation schemes indicated that, to increase the prediction accuracy of a new hybrid line, it would be better to field-test the hybrid line in at least one environment. Our results would be helpful for designing training population and planning field testing in hybrid breeding.
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http://dx.doi.org/10.3390/plants10061174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227059PMC
June 2021

A simple strategy for the detection of Pb(II) and Cu(II) by an electrochemical sensor based on Zn/Ni-ZIF-8/XC-72/Nafion hybrid materials.

Environ Res 2021 Jun 29;202:111605. Epub 2021 Jun 29.

School of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, 453003, China.

In this study, a novel electrochemical sensor for simultaneous detection of Pb(II) and Cu(II) was constructed by using Zn/Ni-ZIF-8/XC-72/Nafion hybrid material as electrode surface modifier. XRD, FT-IR, XPS and SEM were used to study the crystal structure, functional groups, element types and morphologies of the prepared materials. The electrochemical performance of the Zn/Ni-ZIF-8/XC-72/Nafion/GCE sensor were investigated by CV, EIS and DPV. In addition, the effects of various conditions including pH, the type of buffer and the ratio of Zn/Ni-ZIF-8 to XC-72 were also explored for the determination of Pb(II) and Cu(II). Under the optimum conditions, the constructed sensor exhibited outstanding linear response of Pb(II) (0.794-39.6 ppm) and Cu(II) (0.397-19.9 ppm) with detection limits of 0.0150 and 0.0096 ppm, respectively. Finally, the fabricated sensor was further used to detect Pb(II) and Cu(II) in real samples, and the satisfactory recovery was obtained.
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http://dx.doi.org/10.1016/j.envres.2021.111605DOI Listing
June 2021

Reply to "Letter to the Editor" by Cem Yener et al.

Arch Gynecol Obstet 2021 Jun 26. Epub 2021 Jun 26.

Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, 321 Zhongshan Road, Nanjing, 210008, People's Republic of China.

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http://dx.doi.org/10.1007/s00404-021-06135-2DOI Listing
June 2021

Brønsted acid catalysed chemo- and -selective aminomethylation of phenol.

Org Biomol Chem 2021 Jul;19(26):5777-5781

School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China. and Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, East China Normal University, Shanghai 200062, P. R. China.

We have developed a Brønsted acid catalysed highly ortho-selective functionalization of free phenols with readily available N,O-acetals under mild conditions, furnishing various corresponding aminomethylated phenol products in moderate to excellent yields. The salient features of this transformation include mild conditions, good substrate scope, excellent ortho-selectivity, high efficiency, and ease of further transformation.
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http://dx.doi.org/10.1039/d1ob00820jDOI Listing
July 2021

An integrative analysis of genome-wide 5-hydroxymethylcytosines in circulating cell-free DNA detects noninvasive diagnostic markers for gliomas.

Neurooncol Adv 2021 Jan-Dec;3(1):vdab049. Epub 2021 Apr 16.

State Key Laboratory of Medical Neurobiology, School of Basic Medical Sciences, and The Collaborative Innovation Centre for Brain Science, Fudan University, Shanghai, China.

Background: Gliomas, especially the high-grade glioblastomas (GBM), are highly aggressive tumors in the central nervous system (CNS) with dismal clinical outcomes. Effective biomarkers, which are not currently available, may improve clinical outcomes through early detection. We sought to develop a noninvasive diagnostic approach for gliomas based on 5-hydroxymethylcytosines (5hmC) in circulating cell-free DNA (cfDNA).

Methods: We obtained genome-wide 5hmC profiles using the 5hmC-Seal technique in cfDNA samples from 111 prospectively enrolled patients with gliomas and 111 age-, gender-matched healthy individuals, which were split into a training set and a validation set. Integrated models comprised 5hmC levels summarized for gene bodies, long noncoding RNAs (lncRNAs), -regulatory elements, and repetitive elements were developed using the elastic net regularization under a case-control design.

Results: The integrated 5hmC-based models differentiated healthy individuals from gliomas (area under the curve [AUC] = 84%; 95% confidence interval [CI], 74-93%), GBM patients (AUC = 84%; 95% CI, 74-94%), WHO II-III glioma patients (AUC = 86%; 95% CI, 76-96%), regardless of (encoding isocitrate dehydrogenase) mutation status or other glioma-related pathological features such as TERT, TP53 in the validation set. Furthermore, the 5hmC biomarkers in cfDNA showed the potential as an independent indicator from mutation status and worked in synergy with mutation to distinguish GBM from WHO II-III gliomas. Exploration of the 5hmC biomarkers for gliomas revealed relevance to glioma biology.

Conclusions: The 5hmC-Seal in cfDNA offers the promise as a noninvasive approach for effective detection of gliomas in a screening program.
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http://dx.doi.org/10.1093/noajnl/vdab049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209591PMC
April 2021

A study on the effects of mindfulness-based cognitive therapy and loving-kindness mediation on depression, rumination, mindfulness level and quality of life in depressed patients.

Am J Transl Res 2021 15;13(5):4666-4675. Epub 2021 May 15.

Student Affairs Department, Qiqihar Medical University Qiqihar 161006, Heilongjiang, China.

Objective: To analyze the effects of mindfulness-based cognitive therapy (MBCT) plus loving-kindness mediation (LKM) in depressed patients.

Methods: A total of 125 depressed patients diagnosed in the Department of Psychiatry of our hospital were selected as the research subjects and were randomly divided into a control group (n=62) and an observation group (n=63). The control group was treated with conventional psychological intervention, while the observation group was treated with MBCT plus LKM. The therapeutic outcomes were compared between the two groups.

Results: At 2, 4, 6 and 8 weeks after intervention, the Hamilton Depression Rating Scale (HAMD) scores and the scores for introspection and deliberation, forced thinking, rumination of symptoms, treatment, ability and social relationships in the observation group were lower than those in the control group, while Five Facet Mindfulness Questionnaire (FFMQ) scores and the scores for psychology, environment, physiology, social relations, self-acceptance, and self-evaluation in the observation group were higher than those in the control group ( < 0.05).

Conclusion: MBCT plus LKM can effectively improve depression, rumination, mindfulness level, quality of life, the sense of stigma and degree of self-acceptance in depressed patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205847PMC
May 2021

Effects of Microplastics Exposure on the sp. Antioxidant, Immunization and Energy Metabolism Enzyme Activities.

Front Microbiol 2021 4;12:666100. Epub 2021 Jun 4.

Shenzhen Institute of Guangdong Ocean University, Shenzhen, China.

Microplastic pollution in marine environments has increased rapidly in recent years, with negative influences on the health of marine organisms. Scleractinian coral, one of the most important species in the coral ecosystems, is highly sensitive to microplastic. However, whether microplastic causes physiological disruption of the coral, oxidative stress, immunity, and energy metabolism, is unclear. In the present study, the physiological responses of the coral sp. were determined after exposure to polyethylene terephthalate (PET), polyamide 66 (PA66), and polyethylene (PE) microplastic for 96 h. The results showed that there were approximately 4-22 items/nubbin on the surface of the coral skeleton and 2-10 items/nubbin on the inside of the skeleton in the MPs exposure groups. The density of endosymbiont decreased (1.12 × 10-1.24 × 10 cell/cm) in MPs exposure groups compared with the control group. Meanwhile, the chlorophyll content was reduced (0.11-0.76 μg/cm) after MPs exposure. Further analysis revealed that the antioxidant enzymes in coral tissues were up-regulated (Total antioxidant capacity T-AOC 2.35 × 10-1.05 × 10 mmol/mg prot, Total superoxide dismutase T-SOD 3.71-28.67 U/mg prot, glutathione GSH 10.21-10.51 U/mg prot). The alkaline phosphatase (AKP) was inhibited (1.44-4.29 U/mg prot), while nitric oxide (NO) increased (0.69-2.26 μmol/g prot) for cell signal. Moreover, lactate dehydrogenase (LDH) was down-regulated in the whole experiment period (0.19-0.22 U/mg prot), and Glucose-6-phosphate dehydrogenase (G6PDH) for cell the phosphate pentoses pathway was also reduced (0.01-0.04 U/mg port). Results showed that the endosymbiont was released and chlorophyll was decreased. In addition, a disruption could occur under MPs exposure, which was related to anti-oxidant, immune, and energy metabolism.
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http://dx.doi.org/10.3389/fmicb.2021.666100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213336PMC
June 2021

Strongly Coupled 2D Transition Metal Chalcogenide-MXene-Carbonaceous Nanoribbon Heterostructures with Ultrafast Ion Transport for Boosting Sodium/Potassium Ions Storage.

Nanomicro Lett 2021 Apr 22;13(1):113. Epub 2021 Apr 22.

Sino-Russian International Joint Laboratory for Clean Energy and Energy Conversion Technology, International Center of Future Science, College of Physics, Jilin University, Changchun, 130012, People's Republic of China.

Highlights: Unique "Janus" interfacial assemble strategy of 2D MXene nanosheets was proposed firstly. Ternary heterostructure consisting of high capacity transitional metal chalcogenide, high conductive 2D MXene and N rich fungal carbonaceous matrix was achieved for larger radius Na/K ions storages. The highly accessible surfaces and interfaces of the strongly coupled 2D based ternary heterostructures provide superb surficial pseudocapacitive storages for both Na and K ions with low energy barriers was verified. Combining with the advantages of two-dimensional (2D) nanomaterials, MXenes have shown great potential in next generation rechargeable batteries. Similar with other 2D materials, MXenes generally suffer severe self-agglomeration, low capacity, and unsatisfied durability, particularly for larger sodium/potassium ions, compromising their practical values. In this work, a novel ternary heterostructure self-assembled from transition metal selenides (MSe, M = Cu, Ni, and Co), MXene nanosheets and N-rich carbonaceous nanoribbons (CNRibs) with ultrafast ion transport properties is designed for sluggish sodium-ion (SIB) and potassium-ion (PIB) batteries. Benefiting from the diverse chemical characteristics, the positively charged MSe anchored onto the electronegative hydroxy (-OH) functionalized MXene surfaces through electrostatic adsorption, while the fungal-derived CNRibs bonded with the other side of MXene through amino bridging and hydrogen bonds. This unique MXene-based heterostructure prevents the restacking of 2D materials, increases the intrinsic conductivity, and most importantly, provides ultrafast interfacial ion transport pathways and extra surficial and interfacial storage sites, and thus, boosts the high-rate storage performances in SIB and PIB applications. Both the quantitatively kinetic analysis and the density functional theory (DFT) calculations revealed that the interfacial ion transport is several orders higher than that of the pristine MXenes, which delivered much enhanced Na (536.3 mAh [email protected] 0.1 A g) and K (305.6 mAh [email protected] 1.0 A g ) storage capabilities and excellent long-term cycling stability. Therefore, this work provides new insights into 2D materials engineering and low-cost, but kinetically sluggish post-Li batteries.
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http://dx.doi.org/10.1007/s40820-021-00623-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062676PMC
April 2021

Interfacial Engineering of CuO Passivating Contact for Efficient Crystalline Silicon Solar Cells with an AlO Passivation Layer.

ACS Appl Mater Interfaces 2021 Jun 14;13(24):28415-28423. Epub 2021 Jun 14.

CAS Key Lab of Low-Carbon Conversion Science and Engineering, Chinese Academy of Sciences, Shanghai Advanced Research Institute, 99 Haike Road, Zhangjiang Hi-Tech Park, Pudong, Shanghai 201210, China.

Passivating contacts that simultaneously promote carrier selectivity and suppress surface recombination are considered as a promising trend in the crystalline silicon (-Si) photovoltaic industry. In this work, efficient -type -Si (-Si) solar cells with cuprous oxide (CuO) hole-selective contacts are demonstrated. The direct -Si/CuO contact leads to a substoichiometric SiO interlayer and diffusion of Cu into the silicon substrate, which would generate a deep-level impurity behaving as carrier recombination centers. An AlO layer is subsequently employed at the -Si/CuO interface, which not only serves as a passivating and tunneling layer but also suppresses the redox reaction and Cu diffusion at the Si/CuO interface. In conjunction with the high work function of Au and the superior optical property of Ag, a power conversion efficiency up to 19.71% is achieved with a -Si/AlO/CuO/Au/Ag rear contact. This work provides a strategy for reducing interfacial defects and lowering energy barrier height in passivating contact solar cells.
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http://dx.doi.org/10.1021/acsami.1c08258DOI Listing
June 2021

Bioinspired Multi-Stimuli Responsive Actuators with Synergistic Color- and Morphing-Change Abilities.

Adv Sci (Weinh) 2021 08 10;8(16):e2101295. Epub 2021 Jun 10.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu, 610065, China.

The combination of complex perception, defense, and camouflage mechanisms is a pivotal instinctive ability that equips organisms with survival advantages. The simulations of such fascinating multi-stimuli responsiveness, including thigmotropism, bioluminescence, color-changing ability, and so on, are of great significance for scientists to develop novel biomimetic smart materials. However, most biomimetic color-changing or luminescence materials can only realize a single stimulus-response, hence the design and fabrication of multi-stimuli responsive materials with synergistic color-changing are still on the way. Here, a bioinspired multi-stimuli responsive actuator with color- and morphing-change abilities is developed by taking advantage of the assembled cellulose nanocrystals-based cholesteric liquid crystal structure and its water/temperature response behaviors. The actuator exhibits superfast, reversible bi-directional humidity and near-infrared (NIR) light actuating ability (humidity: 9 s; NIR light: 16 s), accompanying with synergistic iridescent appearance which provides a visual cue for the movement of actuators. This work paves the way for biomimetic multi-stimuli responsive materials and will have a wide range of applications such as optical anti-counterfeiting devices, information storage materials, and smart soft robots.
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http://dx.doi.org/10.1002/advs.202101295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373155PMC
August 2021

Discovery of as a Potent and Orally Bioavailable Inhibitor of the WDR5-Mixed Lineage Leukemia 1 Protein-Protein Interaction for the Treatment of MLL Fusion Leukemia.

J Med Chem 2021 06 9;64(12):8221-8245. Epub 2021 Jun 9.

Jiangsu Key Laboratory of Drug Design and Optimization and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.

WD repeat-containing protein 5 (WDR5) is essential for the stability and methyltransferase activity of the mixed lineage leukemia 1 (MLL1) complex. Dysregulation of the gene is associated with human acute leukemias, and the direct disruption of the WDR5-MLL1 protein-protein interaction (PPI) is emerging as an alternative strategy for MLL-rearranged cancers. Here, we represent a new aniline pyrimidine scaffold for WDR5-MLL1 inhibitors. A comprehensive structure-activity analysis identified a potent inhibitor (), with an IC of 29 nM in a competitive fluorescence polarization assay and a value of 72.9 nM for the WDR5 protein. Compound selectively inhibited MLL histone methyltransferase activity and the proliferation of MLL translocation-harboring cells. Furthermore, displayed good pharmacokinetic properties and suppressed the growth of MV4-11 xenograft tumors in mice after oral administration, first verifying the efficacy of targeting the WDR5-MLL1 PPI by small molecules.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00091DOI Listing
June 2021

Reactive oxygen species-sensitive polymeric nanocarriers for synergistic cancer therapy.

Acta Biomater 2021 08 28;130:17-31. Epub 2021 May 28.

Guangzhou First People's Hospital, School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China; National Engineering Research Center for Tissue Restoration and Reconstruction, and Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, Guangdong 510006, China. Electronic address:

Reactive oxygen species (ROS)-responsive nanocarriers have aroused widespread interest in recent years. On the one hand, a high ROS level has been detected in many types of tumor cells. On the other hand, ROS generation is also induced during photodynamic, sonodynamic, or chemodynamic therapy. In addition, multiple types of polymers are sensitive to ROS. Therefore, numerous ROS-responsive polymeric nanocarriers with unique ROS-responsive characteristics have been developed. This review discusses ROS-sensitive polymeric nanocarriers to improve drug delivery efficacy. In particular, ROS-responsive nanocarriers for synergistic cancer therapy are highlighted. The development of novel ROS-sensitive nanocarriers holds great potential for combining ROS-mediated therapy, such as photodynamic therapy, and other therapies to achieve synergistic anticancer efficacy. STATEMENT OF SIGNIFICANCE: Reactive oxygen species (ROS)-responsive nanocarriers aroused widespread interest in recent years. On the one hand, a high level of ROS has been found in many types of tumor cells. On the other hand, the ROS generation can also be induced during the photodynamic, sonodynamic, or chemodynamic therapy. Besides, multiple types of polymers were sensitive to the ROS. Therefore, numerous ROS-responsive polymeric nanocarriers with unique ROS responsive characteristics have been developed. This review focuses on the ROS-sensitive polymeric nanocarriers to improve drug delivery efficacy for synergistic cancer therapy.
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http://dx.doi.org/10.1016/j.actbio.2021.05.023DOI Listing
August 2021

An MRI-trackable therapeutic nanovaccine preventing cancer liver metastasis.

Biomaterials 2021 07 19;274:120893. Epub 2021 May 19.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Center for Bionanoengineering, And College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China. Electronic address:

Cancer vaccines consisting of tumor-associated antigens (TAAs) can initiate a powerful antitumor immune response through antigen-presenting cells, such as dendritic cells (DCs) and macrophages, and have shown great potential in cancer prevention and therapy. However, poor anticancer efficacy and an uncertain immunization process have hitherto limited the application of cancer vaccines. Herein, a multifunctional nanovaccine comprising ovalbumin (OVA), MnO, and polydopamine (OMPN) was prepared by a facile one-pot method. OMPN displayed excellent anticancer efficacy against an orthotopic melanoma and could also prevent liver metastasis in a tumor re-challenge mice model. Additionally, the migration behavior of DCs in the inguinal lymph node after vaccination was tracked by MRI contrasted with OMPN, indicating successful DC activation and immune response. The superior anticancer efficacy, especially the high efficiency against tumor metastasis, and the capability of tracking the immunization process make OMPN a very promising multifunctional nanovaccine for cancer therapy.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120893DOI Listing
July 2021

Post-annealing Effect on Optical and Electronic Properties of Thermally Evaporated MoO Thin Films as Hole-Selective Contacts for p-Si Solar Cells.

Nanoscale Res Lett 2021 May 19;16(1):87. Epub 2021 May 19.

CAS Key Lab of Low-Carbon Conversion Science and Engineering, The Interdisciplinary Research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Zhangjiang Hi-Tech Park, Pudong, Shanghai, 201210, China.

Owing to its large work function, MoO has been widely used for hole-selective contact in both thin film and crystalline silicon solar cells. In this work, thermally evaporated MoO films are employed on the rear sides of p-type crystalline silicon (p-Si) solar cells, where the optical and electronic properties of the MoO films as well as the corresponding device performances are investigated as a function of post-annealing treatment. The MoO film annealed at 100 °C shows the highest work function and proves the best hole selectivity based on the results of energy band simulation and contact resistivity measurements. The full rear p-Si/MoO/Ag-contacted solar cells demonstrate the best performance with an efficiency of 19.19%, which is the result of the combined influence of MoO's hole selectivity and passivation ability.
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http://dx.doi.org/10.1186/s11671-021-03544-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134614PMC
May 2021

GmMs1 encodes a kinesin-like protein essential for male fertility in soybean (Glycine max L.).

J Integr Plant Biol 2021 Jun;63(6):1054-1064

The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement (MOA), Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

The application of heterosis is a promising approach for greatly increasing yield in soybean (Glycine max L.). Nuclear male sterility is essential for hybrid seed production and the utilization of heterosis. Here we report the cloning of the gene underlying the soybean male-sterile mutant ms-1, which has been widely used for recurrent selection in soybean breeding programs. We initially delimited the ms1 locus to a 16.15 kb region on chromosome 13, based on SLAF_BSA sequencing followed by genotyping of an F population segregating for the locus. Compared with the same region in fertile plants, the mutant region lacks a sequence of approximately 38.7 kb containing five protein-coding genes, including an ortholog of the kinesin-like protein gene NACK2, named GmMs1. The GmMs1 knockout plants generated via CRISPR/Cas-mediated gene editing displayed a complete male-sterile phenotype. Metabolic profiling showed that fertile anthers accumulated starch and sucrose normally, whereas sterile anthers had higher anthocyanin levels and lower flavonoid levels and lower antioxidant enzyme activities. These results provide insights into the molecular mechanisms governing male sterility and demonstrate that GmMs1 could be used to create male-sterile lines through targeted mutagenesis. These findings pave the way for designing seed production technology and an intelligent male-sterile line system to utilize heterosis in soybean.
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http://dx.doi.org/10.1111/jipb.13110DOI Listing
June 2021

Relationship between Adverse Childhood Experiences and substance use in youth offenders in Singapore.

Child Abuse Negl 2021 Jul 21;117:105072. Epub 2021 Apr 21.

Probation and Community Rehabilitation Services, Ministry of Social and Family Development, 1 Kay Siang Rd, #01-10, 248922, Singapore. Electronic address:

Background: Adverse Childhood Experiences are associated with worse outcomes in delinquency and substance use.

Objective: Current research is overwhelmingly from Western perspectives, leaving a gap in non-Western, low crime-rate jurisdictions. Moreover, there exists a gap in characterizing the effect of ACE frequency on delinquency. We extend existing research by examining relationships between ACE and substance use in youth offenders in Singapore.

Participants And Setting: The study included 790 youth offenders (669 males, M = 17.59 years) from a longitudinal study on youth offending.

Methods: Multiple regression was performed to examine relationships between self-reported ACEs and substance use. Latent Class Analysis was conducted to identify classes of substance use onset. The relationship between these classes and cumulative ACEs and ACE frequency were then tested using multiple regression.

Results: Youth offenders who consume alcohol (B = 0.66, p = .002) and illicit drugs (B = 0.38, p = .02) had more cumulative and more frequent ACEs than those who do not. Moreover, we found a positive relationship between ACEs and substance use frequency. Those who started taking substances in childhood had significantly more ACEs and had worse drug dependency problems than those who started later (t = 5.93, p < .0001). Additionally, there was a positive relationship between ACEs and drug use dependency (B = 0.11, p = .03).

Conclusions: Our findings highlight the importance of ACEs as risk factors for substance use. This underscores the need for comprehensive screening and treatment of ACEs and substance use in the rehabilitative context.
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http://dx.doi.org/10.1016/j.chiabu.2021.105072DOI Listing
July 2021
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