Publications by authors named "DongFang Li"

163 Publications

The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson's disease more effectively than the GLP-1 single-receptor agonist NLY01.

Brain Behav 2021 Jun 14. Epub 2021 Jun 14.

Second Hospital, Neurology Department, Shanxi Medical University, Taiyuan, Shanxi Province, People's Republic of China.

The GLP-1 receptor agonist exendin-4 has recently shown good effects in a phase II clinical trial in Parkinson's disease (PD) patients. Here, a comparison of the new GLP-1/GIP dual receptor agonist DA5-CH and NLY01, a 40 kDa pegylated form of exendin-4, on motor impairments and reducing inflammation in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) PD mouse model is provided. The drug groups received either DA5-CH or NLY01 (25 nmol/kg) i.p. after daily MPTP intraperitoneal injection. Both drugs showed improvements in motor activity, open field experiments, rotarod tests, and gait analysis, but DA5-CH was more potent. Tyrosine hydroxylase expression in dopaminergic neurons was much reduced by MPTP and improved by DA5-CH, while NLY01 showed weak effects. When analyzing levels of α-synuclein (α-Syn), DA5-CH reduced levels effectively while NLY01 had no effect. When measuring the levels of the inflammation markers Toll-like receptor 4 (TLR4), specific markers of microglia activation (Iba-1), the marker of astrocyte activation glial fibrillary acidic protein (GFAP), nuclear factor-κB (NF-κB), tumor necrosis factor (TNF-α), and transforming growth factor β1 (TGF-β1), DA5-CH was very effective in reducing the chronic inflammation response, while NLY01 did not show significant effects. Levels of key growth factors such as Glial cell-derived neurotrophic factor (GDNF) and Brain-derived neurotrophic factor (BDNF) were much reduced by MPTP, and DA5-CH was able to normalize levels in the brain, while NLY01 showed little effect. The levels of pro-inflammatory cytokines (IL-6 and IL-Iβ) were much reduced by DA5-CH, too, while NLY01 showed no effect. In a separate experiment, we tested the ability of the two drugs to cross the blood-brain barrier. After injecting fluorescin-labelled peptides peripherally, the fluorescence in brain tissue was measured. It was found that the pegylated NLY01 peptide did not cross the BBB in meaningful quantities while exendin-4 and the dual agonist DA5-CH did. The results show that DA5-CH shows promise as a therapeutic drug for PD.
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http://dx.doi.org/10.1002/brb3.2231DOI Listing
June 2021

First molecular characterization of Enterocytozoon bieneusi in children and calves in Bangladesh.

Transbound Emerg Dis 2021 Jun 10. Epub 2021 Jun 10.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

Enterocytozoon bieneusi is a widespread opportunistic pathogen found in humans and domestic animals, including cattle that poses a public health risk. This study was performed to evaluate the prevalence, genotypic diversity, and zoonotic potential of E. bieneusi among children and calves in Bangladesh. A total of 998 fecal samples were collected from children (n = 299) and calves (n = 699) and screened by nested PCR and sequencing of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi infection was 6.4% in children and 7.9% in calves. ITS sequence analysis of 74 isolates revealed 10 genotypes, including eight known genotypes (A, D, Type IV, PigEBITS7, I, J, BEB4, and BEB6) and two new genotypes (BANEB1 and BANEB3). Specifically, genotypes A, D, Type IV, PigEBITS7, BANEB1, and BANEB3, and genotypes D, PigEBITS7, I, J, BEB4, and BEB6 were detected in children and calves, respectively. Among them, genotypes D and I were dominant genotypes in children and calves, respectively. The genotypes D and PigEBITS7 were found in both children and calves, with PigEBITS7 being observed for the first time in calves. In phylogenetic analysis, six genotypes (A, D, Type IV, PigEBITS7, BANEB1, and BANEB3), detected in 39.2% of the isolates, belonged to zoonotic Group 1. The remaining four genotypes I, J, BEB4, and BEB6 were clustered in Group 2 and are common members of the group with zoonotic potential. To the best of our knowledge, this study provides the first report of E. bieneusi infection in calves in Bangladesh and also the first molecular characterization of the parasite in children and calves in this country. Two new genotypes in children have been found, which is noteworthy. Furthermore, the presence of zoonotic genotypes indicates that cattle may serve as reservoirs for E. bieneusi, which can be a source of human microsporidiosis.
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http://dx.doi.org/10.1111/tbed.14187DOI Listing
June 2021

Analysis of the curative effect of percutaneous kyphoplasty in the treatment of osteoporotic vertebral compression fracture with intravertebral clefts.

Medicine (Baltimore) 2021 Jun;100(22):e25996

Department of Spine Surgery, Luoyang Orthopedic Hospital of Henan Province, South Qiming Road, Luoyang, China.

Abstract: Kummell's disease is a delayed vertebral collapse fracture caused by posttraumatic osteonecrosis. It is a special type of osteoporotic vertebral fracture in the elderly. This study compares and analyzes the difference in the curative effect of 2 kinds of osteoporotic vertebral compression fracture (OVCF) in the presence of fracture or not in the vertebral body, and provides a clinical reference for the application of percutaneous kyphoplasty (PKP).This research is a kind of retrospective analysis from January 2012 to January 2015, PKP was used to treat 165 patients with osteoporotic vertebral compression fracture. The patients were divided into 2 groups: Intravertebral clefts group (group A) and none-intravertebral clefts group in vertebral body (group B). Bone mineral density (BMD), bone cement injection (BCI), Visual analogue scale (VAS) score before and after surgery, anterior, central and posterior height of vertebral body (before and after surgery) and Cobb angle of injured vertebra (before and after surgery) were compared between the 2 groups.Surgeries for 165 patients in the 2 groups were successfully completed, and 226 fractured vertebrae were performed through bilateral puncture approach to strengthen the vertebral body. Intraoperative injection of bone cement (ml) was 4.25 + 1.29 (range: 2.6-7.8). There were statistically significant differences in bone cement injection quantity between the 2 groups (P < .05), and in bone cement leakage (P > .05) as well as the Postoperative VAS score (P < .05). However, There was no statistical difference in VAS score before surgery between the 2 groups (P > .05). The results indicated that the pain relief degree of OVCF patients without intravertebral clefts is better than that in the vertebral body. No statistical difference was found in Cobb Angle before and after surgery (P > .05), as well as the correction rate of the injured vertebrae before and after surgery (P > .05). There was no statistical difference in the degree of recovery of the anterior, middle and posterior margins of the injured vertebrae after surgery (P > .05).PKP treatment led to better degree of pain relief in OVCF patients without intravertebral clefts, and less bone cement was injected into the surgery.
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http://dx.doi.org/10.1097/MD.0000000000025996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183815PMC
June 2021

LPS induces fibroblast-like synoviocytes RSC-364 cells to pyroptosis through NF-κB mediated dual signalling pathway.

J Mol Histol 2021 Jun 3. Epub 2021 Jun 3.

Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Shandong University, Wenhua West Road 44-1, Jinan, 250012, China.

Rheumatoid arthritis (RA) is a chronic, progressive, and systemic inflammatory joint disease characterized by synovial inflammation and joint damage. Abnormal activation of fibroblast-like synoviocytes is an initial event of synovial inflammation and joint damage, which can significantly aggravate the progression of RA. Clinical studies have shown that synovitis may be associated with pyroptosis. Therefore, this study is mainly aim for exploring the underlying mechanisms of relationship between inflammation and pyroptosis during synovitis. A cell model of synovitis was constructed by stimulating synovial fibroblasts RSC-364 cells with lipopolysaccharide (LPS). In vitro, we found that LPS can induce pyroptosis of synovial fibroblasts through NOD-like receptor pyrin domain-3/caspase-1/gasdermin D and caspase-3/gasdermin E two signaling pathways, and these two signaling pathways can promote each other. In addition, NF-κB signaling pathway, as the upstream of these two pathways, is involved in regulating the pyroptosis of synovial fibroblast. These results suggest that pyroptosis may be triggered during the occurrence of RA. We hope to provide a new perspective for the study of RA and a new therapeutic target for clinical treatment of RA.
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http://dx.doi.org/10.1007/s10735-021-09988-8DOI Listing
June 2021

Clinical Features, Risk Factors, and Therapy of Epithelial Keratitis after Cataract Surgery.

J Ophthalmol 2021 6;2021:6636228. Epub 2021 May 6.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, China.

Purpose: The study aimed to assess the clinical characteristics, risk factors, and therapy of epithelial keratitis after cataract surgery.

Methods: Medical data of 89 consecutive patients who developed epithelial keratitis after cataract surgery, including 37 patients with diabetes mellitus (37 eyes) and 52 patients without diabetes mellitus (52 eyes), were retrospectively reviewed. The clinical characteristics, risk factors, and therapy in those patients were evaluated.

Results: The preoperative tear film function determined by the tear breakup time, meibomian gland atrophy score, and low tear meniscus height in diabetic patients was poorer than nondiabetic patients ( < 0.001). Of diabetic patients, 83.78% (31/37) had been diagnosed with meibomian gland dysfunction before cataract surgery and treated with topical nonsteroidal anti-inflammatory drugs after cataract surgery for 44.69 ± 10.51 days, compared to 42.31% (22/52) of nondiabetic patients receiving the topical nonsteroidal anti-inflammatory treatment for 33.35 ± 5.16 days (both < 0.001). Epithelial lesions progressed within three to four days following cataract surgery in 59.46% (22/37) of diabetic patients, versus 30.77% (16/52) of the nondiabetic patients (=0.025). Patients with combined meibomian gland dysfunction and epithelial defects accounted for 48.65% (18/37) in the diabetic group and 25.00% (13/52) in the nondiabetic group ( < 0.001). In vivo confocal microscopy showed absence of subbasal never fibers in eyes with epithelial defects, and central corneal sensation was also significantly depressed in those eyes, but there was no significant difference between the two groups (=0.227). Corneal ulceration and herpes simplex keratitis were found in 2.70% (1/37) and 5.41% (2/37) of diabetic patients, respectively. Amniotic membrane transplantation was required in 32.43% (12/37) of patients in the diabetic group, and the proportion was higher than 1.92% (1/52) in the nondiabetic group ( < 0.001). Average healing time of the corneal epithelium in the diabetic group was 40.62 ± 20.0 days, much longer than 21.74 ± 6.94 days in the nondiabetic group (=0.002).

Conclusion: Epithelial keratitis after cataract surgery in diabetic patients has the characteristics of rapid development, severe epithelial damage, and slow repair of the corneal epithelium. Amniotic membrane transplantation is a good choice for persistent epithelial defects associated with such epithelial keratitis. Attention should be paid to the tear film function and use of topical nonsteroidal anti-inflammatory drugs in patients undergoing cataract surgery.
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http://dx.doi.org/10.1155/2021/6636228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121559PMC
May 2021

Analyses of key mRNAs and lncRNAs for different osteo-differentiation potentials of periodontal ligament stem cell and gingival mesenchymal stem cell.

J Cell Mol Med 2021 May 24. Epub 2021 May 24.

Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, China.

Both human periodontal ligament stem cells (hPDLSCs) and human gingival mesenchymal stem cells (hGMSCs) are candidate seed cells for bone tissue engineering, but the osteo-differentiation ability of the latter is weaker than the former, and the mechanisms are unknown. To explore the potential regulation of mRNAs and long non-coding RNAs (lncRNAs), this study obtained the gene expression profiles of hPDLSCs and hGMSCs in both undifferentiated and osteo-differentiated conditions by microarray assay and then analysed the common and specific differentially expressed mRNAs and lncRNAs in hPDLSCs and hGMSCs through bioinformatics method. The results showed that 275 mRNAs and 126 lncRNAs displayed similar changing patterns in hPDLSCs and hGMSCs after osteogenic induction, which may regulate the osteo-differentiation in both types of cells. In addition, the expression of 223 mRNAs and 238 lncRNAs altered only in hPDLSCs after osteogenic induction, and 177 mRNAs and 170 lncRNAs changed only in hGMSCs. These cell-specific differentially expressed mRNAs and lncRNAs could underlie the different osteo-differentiation potentials of hPDLSCs and hGMSCs. Finally, dickkopf Wnt signalling pathway inhibitor 1 (DKK1) was proved to be one regulator for the weaker osteo-differentiation ability of hGMSCs through validation experiments. We hope these results help to reveal new mRNAs-lncRNAs-based molecular mechanism for osteo-differentiation of hPDLSCs and hGMSCs and provide clues on strategies for improving stem cell-mediated bone regeneration.
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http://dx.doi.org/10.1111/jcmm.16571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256345PMC
May 2021

HCMB: A stable and efficient algorithm for processing the normalization of highly sparse Hi-C contact data.

Comput Struct Biotechnol J 2021 27;19:2637-2645. Epub 2021 Apr 27.

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430000, China.

The high-throughput genome-wide chromosome conformation capture (Hi-C) method has recently become an important tool to study chromosomal interactions where one can extract meaningful biological information including P(s) curve, topologically associated domains, A/B compartments, and other biologically relevant signals. Normalization is a critical pre-processing step of downstream analyses for the elimination of systematic and technical biases from chromatin contact matrices due to different mappability, GC content, and restriction fragment lengths. Especially, the problem of high sparsity puts forward a huge challenge on the correction, indicating the urgent need for a stable and efficient method for Hi-C data normalization. Recently, some matrix balancing methods have been developed to normalize Hi-C data, such as the Knight-Ruiz (KR) algorithm, but it failed to normalize contact matrices with high sparsity. Here, we presented an algorithm, Hi-C Matrix Balancing (HCMB), based on an iterative solution of equations, combining with linear search and projection strategy to normalize the Hi-C original interaction data. Both the simulated and experimental data demonstrated that HCMB is robust and efficient in normalizing Hi-C data and preserving the biologically relevant Hi-C features even facing very high sparsity. HCMB is implemented in Python and is freely accessible to non-commercial users at GitHub: https://github.com/HUST-DataMan/HCMB.
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http://dx.doi.org/10.1016/j.csbj.2021.04.064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120939PMC
April 2021

Collaborative obstacle avoidance algorithm of multiple bionic snake robots in fluid based on IB-LBM.

ISA Trans 2021 May 10. Epub 2021 May 10.

Beijing Institute of Technology, Beijing, China. Electronic address:

This paper presents a collaborative obstacle avoidance algorithm of multiple bionic snake robots in fluid based on IB-LBM. The method can make the multiple bionic snake robots avoid different obstacles in the fluid under the control of the improved Serpenoid curve function. The proposed method has high parallelism, can simulate the complex non-linear phenomenon of the multiple snake robots, deal with the complex boundary conditions of the robot, and reduce the conversion of the computational grid. Firstly, a non-linear fluid model is established by LBM, which solves the non-linear problem that the classical Navier-Stokes equations cannot explain the random motion. Secondly, the force source boundary model of multiple bionic snake robots is established by IBM, which saves the calculation time, improves the calculation efficiency and system stability. After that, each bionic snake robot is given a special force to make the robots collaborate with each other and non-colliding with each other in the process of the obstacle avoidance. Finally, through simulation experiments, the trajectory of multiple bionic snake robots avoiding different number of the obstacles in the fluid is analyzed and the collaborative obstacle avoidance process of multiple bionic snake robots in fluid is observed. The validity of the collaborative obstacle avoidance algorithm of multiple bionic snake robots in fluid based on the IB-LBM is verified.
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http://dx.doi.org/10.1016/j.isatra.2021.04.048DOI Listing
May 2021

β-Glucan from Saccharomyces cerevisiae alleviates oxidative stress in LPS-stimulated RAW264.7 cells via Dectin-1/Nrf2/HO-1 signaling pathway.

Cell Stress Chaperones 2021 Jul 21;26(4):629-637. Epub 2021 Apr 21.

College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, China.

β-Glucan from Saccharomyces cerevisiae has been described to be effective antioxidants, but the specific antioxidation mechanism of β-glucan is unclear. The objectives of this research were to determine whether the β-glucan from Saccharomyces cerevisiae could regulate oxidative stress through the Dectin-1/Nrf2/HO-1 signaling pathway in lipopolysaccharides (LPS)-stimulated RAW264.7 cells. In this study, we examined the effects of β-glucan on the enzyme activity or production of oxidative stress indicators in LPS-stimulated RAW264.7 cells by biochemical analysis and the protein expression of key factors of Dectin-1/Nrf2/HO-1 signaling pathway by immunofluorescence and western blot. The biochemical analysis results showed that β-glucan increased the LPS-induced downregulation of enzyme activity of intracellular heme oxygenase (HO), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while decreasing the production of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, immunofluorescence results showed that β-glucan can activate the nuclear factor erythroid 2-related factor 2 (Nrf2). The antioxidant mechanism study indicated that β-glucan activated dendritic-cell-associated C-type lectin 1 (Dectin-1) receptors mediated Nrf2/HO-1 signaling pathway, thereby downregulating the production of ROS and thus produced the antioxidant effects in LPS-stimulated RAW 264.7 cells. In conclusion, these results indicate that β-glucan potently alleviated oxidative stress via Dectin-1/Nrf2/HO-1 in LPS-stimulated RAW 264.7 cells.
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http://dx.doi.org/10.1007/s12192-021-01205-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275741PMC
July 2021

CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples.

Parasit Vectors 2021 Apr 20;14(1):208. Epub 2021 Apr 20.

College of Animal Science and Veterinary Medicine, Longzihu Campus of Henan Agricultural University, No. 15 Longzihu University Area, Zhengzhou New District, Zhengzhou, 450046, People's Republic of China.

Background: Cryptosporidium parvum is an enteric protozoan parasite with zoonotic importance and can cause cryptosporidiosis in humans as well as domestic and wild animals worldwide. The IId subtype family (SF) is one of the most prevalent subtypes of C. parvum. Some clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein systems have been developed to detect nucleic acid with high flexibility, sensitivity and specificity.

Methods: By integrating recombinase polymerase amplification and the Cas12a/crRNA trans-cleavage system (termed ReCTC), we established end-point diagnostics by observing fluorescence readouts with the naked eye under blue light and on-site diagnostics using a lateral flow strip (LFS) biosensor.

Results: Our ReCTC-based diagnoses can detect as little as a single copy of a cloned C. parvum 60-kDa glycoprotein (GP60) gene, 10 oocysts per gram (OPG), clinical fecal sample without tedious extraction of genomic DNA and have no cross-reactivity with other SFs of C. parvum or other common enteric parasitic protozoa.

Conclusions: This study provided a new strategy for direct identification of the IId SF of C. parvum free of highly trained operators and expensive special equipment.
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http://dx.doi.org/10.1186/s13071-021-04709-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056104PMC
April 2021

Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections.

Parasitol Int 2021 Aug 16;83:102351. Epub 2021 Apr 16.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Animal Epidemical Disease and Infectious Zoonoses, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei 430070, China. Electronic address:

Babesia microti is one of the most important pathogens causing humans and rodents babesiosis-an emerging tick-borne disease that occurs worldwide. At present, the gold standard for the detection of Babesia is the microscopic examination of blood smears, but this diagnostic test has several limitations. The recombinase polymerase amplification with lateral flow (LF-RPA) assay targeting the mitochondrial cytochrome oxidase subunit I (cox I) gene of B. microti was developed in this study. The LF-RPA can be performed within 10-30 min, at a wide range of temperatures between 25 and 45 °C, which is much faster and easier to perform than conventional PCR. The results showed that the LF-RAP can detect 0.25 parasites/μl blood, which is 40 times more sensitive than the conventional PCR based on the V4 variable region of 18S rRNA. Specificity assay showed no cross-reactions with DNAs of related apicomplexan parasites and their host. The applicability of the LF-RPA method was further evaluated using two clinical human samples and six experimental mice samples, with seven samples were positively detected, while only three of them were defined as positive by conventional PCR. These results present the developed LF-RPA as a new simple, specific, sensitive, rapid and convenient method for diagnosing infection with B. microti. This novel assay was the potential to be used in field applications and large-scale sample screening.
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http://dx.doi.org/10.1016/j.parint.2021.102351DOI Listing
August 2021

The Strain Rate Sensitivity and Creep Behavior for the Tripler Plane of Potassium Dihydrogen Phosphate Crystal by Nanoindentation.

Micromachines (Basel) 2021 Mar 30;12(4). Epub 2021 Mar 30.

College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.

As an excellent multifunctional single crystal, potassium dihydrogen phosphate (KDP) is a well-known, difficult-to-process material for its soft-brittle and deliquescent nature. The surface mechanical properties are critical to the machining process; however, the characteristics of deformation behavior for KDP crystals have not been well studied. In this work, the strain rate effect on hardness was investigated on the mechanically polished tripler plane of a KDP crystal relying on nanoindentation technology. By increasing the strain rate from 0.001 to 0.1 s, hardness increased from 1.67 to 2.07 GPa. Hence, the strain rate sensitivity was determined as 0.053, and the activation volume of dislocation nucleation was 169 Å. Based on the constant load-holding method, creep deformation was studied at various holding depths at room temperature. Under the spherical tip, creep deformation could be greatly enhanced with increasing holding depth, which was mainly due to the enlarged holding strain. Under the self-similar Berkovich indenter, creep strain could be reduced at a deeper location. Such an indentation size effect on creep deformation was firstly reported for KDP crystals. The strain rate sensitivity of the steady-state creep flow was estimated, and the creep mechanism was qualitatively discussed.
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http://dx.doi.org/10.3390/mi12040369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067031PMC
March 2021

ATG14 and RB1CC1 play essential roles in maintaining muscle homeostasis.

Autophagy 2021 Apr 14:1-10. Epub 2021 Apr 14.

Departments of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Defects in macroautophagy/autophagy are implicated in the pathogenesis of neuromuscular and heart diseases. To precisely define the roles of autophagy-related genes in skeletal and cardiac muscles, we generated muscle-specific and conditional knockout (cKO) mice by using and compared their phenotypes to those of -conditional double-knockout (cDKO) mice. -cKO mice developed hypertrophic cardiomyopathy, which was associated with abnormal accumulation of autophagic cargoes in the heart and early mortality. Skeletal muscles of both -cKO and -cKO mice showed features of autophagic vacuolar myopathy with ubiquitin SQSTM1 deposits, but only those of -cKO mice showed TARDBP/TDP-43 pathology and other features of the inclusion body myopathy-like disease we previously described in -cDKO mice. Herein, we highlight tissue-specific differences between skeletal and cardiac muscles in their reliance on core autophagy proteins and unique roles for ULK1-ULK2 and RB1CC1 among these proteins in the development of TARDBP pathology.:AVM: autophagic vacuolar myopathy; cDKO: conditional double knockout; cKO: conditional knockout; H&E: hematoxylin and eosin; IBM: inclusion body myopathy; mtDNA: mitochondrial DNA; PFA: paraformaldehyde; RNP: ribonucleoprotein; TBST: Tris-buffered saline with 0.2% Triton X-100.
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http://dx.doi.org/10.1080/15548627.2021.1911549DOI Listing
April 2021

microRNA-130b-3p Contained in MSC-Derived EVs Promotes Lung Cancer Progression by Regulating the FOXO3/NFE2L2/TXNRD1 Axis.

Mol Ther Oncolytics 2021 Mar 20;20:132-146. Epub 2020 Sep 20.

Department of Thoracic Surgery, Shenzhen Hospital, Southern Medical University, Shenzhen 518101, P.R. China.

This study aimed to explore the molecular mechanism by which mesenchymal stem cells (MSCs) mediate lung cancer progression. Extracellular vesicles (EVs) were isolated from transfected or untransfected MSCs, and were co-cultured with lung cancer cells with/without microRNA-130b-3p (miR-130b-3p) inhibitor, mimic, overexpression plasmids of FOXO3/NFE2L2, or shRNAs. CCK-8 assay, colony formation, transwell assay, and flow cytometry were carried out to determine the biological functioning of lung cancer cells. Furthermore, FOXO3, Keap1, NFE2L2, and TXNRD1 expression was determined by qRT-PCR and western blot analysis. A tumor xenograft mouse model was used to determine role of EVs-miR-130b-3p and its target FOXO3 in lung cancer progression . miR-130b-3p was highly expressed in lung cancer tissues and MSC-derived EVs. Moreover, the MSC-derived EVs transferred miR-130b-3p to lung cancer cells to promote cell proliferation, migration, and invasion while repress cell apoptosis. miR-130b-3p directly targeted FOXO3, and FOXO3 elevated Keap1 expression to downregulate NFE2L2, thus inhibiting TXNRD1. FOXO3 overexpression or silencing of NFE2L2 or TXNRD1 diminished lung cancer cell proliferation, invasion, and migration but enhanced apoptosis. EV-delivered miR-130b-3p or FOXO3 silencing promoted lung cancer progression . In summary, MSC-derived EVs with upregulated miR-130b-3p suppressed FOXO3 to block the NFE2L2/TXNRD1 pathway, thus playing an oncogenic role in lung cancer progression.
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http://dx.doi.org/10.1016/j.omto.2020.09.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851484PMC
March 2021

The novel GLP-1/GIP dual agonist DA3-CH is more effective than liraglutide in reducing endoplasmic reticulum stress in diabetic rats with cerebral ischemia-reperfusion injury.

Nutr Metab Cardiovasc Dis 2021 01 12;31(1):333-343. Epub 2020 Sep 12.

Second Hospital, Neurology Department, Shanxi Medical University, Taiyuan 030001, Shanxi province, PR China; Research and Experimental Center, Henan University of Chinese Medicine, Zhengzhou 450046, Henan province, PR China.

Background And Aims: Diabetes is one of the most important risk factors and comorbidities of ischemic stroke. Endoplasmic reticulum stress (ERS) is considered to be the major injury mechanism of ischemic stroke with diabetes. Studies have found that incretin can inhibit ERS in ischemia-reperfusion injury of the liver and heart. We aimed to explore the effects of GLP-1/GIP double agonist DA3-CH and GLP-1 single agonist liraglutide on ERS and apoptosis in diabetic rats with cerebral ischemia-reperfusion injury.

Methods And Results: 72 Sprague-Dawley (SD) male rats were randomly divided into 4 groups: ① blank group (Sham group, n = 18); model group (Saline group, n = 18); DA3 treatment group (DA3 group, n = 18); liraglutide treatment group (Lir group, n = 18). The Sham group was not given any treatment and was only raised in the same environment as the other groups. The remaining 3 groups used STZ-induced diabetes models. After the successful membrane formation of diabetes, DA3-CH and liraglutide (10 mmol/kg, once-daily for 14 days) were injected intraperitoneally. Thereafter, rats were subjected to middle cerebral artery occlusion followed by 24-h reperfusion. Animals were evaluated for neurologic deficit score, infarct volume, and biomarker analyses of the brain after ischemia. The DA3-CH-treated and liraglutide-treated groups showed significantly reduced scores of neurological dysfunction and cerebral infarction size, and reduced the expression of ERS markers GRP78, CHOP and Caspase-12, and the expression of apoptosis marker bax. Anti-apoptotic markers bcl-2 and neuronal numbers increased significantly.

Conclusions: DA3-CH and liraglutide have obvious neuroprotective effects in a rat model of cerebral ischemia-reperfusion injury with diabetes, which can reduce the infarct size and the neurological deficit score. Their exert neuroprotective effects in a rat model of cerebral ischemia-reperfusion injury with diabetes by inhibiting endoplasmic reticulum stress and thereby reducing apoptosis. DA3 is better than liraglutide.
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http://dx.doi.org/10.1016/j.numecd.2020.09.002DOI Listing
January 2021

Oxaliplatin induces the PARP1-mediated parthanatos in oral squamous cell carcinoma by increasing production of ROS.

Aging (Albany NY) 2021 01 20;13(3):4242-4257. Epub 2021 Jan 20.

Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.

Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors worldwide, and its prognosis is still not optimistic. Oxaliplatin is a type of platinum chemotherapeutic agent, but its treatment effects on OSCC and molecular mechanisms have not been fully elucidated. Parthanatos, a unique form of cell death, plays an important role in a variety of physiological and pathological processes. This study aims to investigate whether oxaliplatin inhibits OSCC by inducing parthanatos. Our results showed that oxaliplatin inhibited the proliferation and migration of OSCC cells , and also inhibited the tumorigenesis . Further experiments proved that oxaliplatin induced parthanatos in OSCC cells, characterized by depolarization of the mitochondrial membrane potential, up-regulation of PARP1, AIF and MIF in the nucleus, as well as the nuclear translocation of AIF. Meanwhile, PARP1 inhibitor rucaparib and siRNA against PARP1 attenuated oxaliplatin-induced parthanatos in OSCC cells. In addition, we found that oxaliplatin caused oxidative stress in OSCC cells, and antioxidant NAC not only relieved oxaliplatin-induced overproduction of reactive oxygen species (ROS) but also reversed parthanatos caused by oxaliplatin. In conclusion, our results indicate that oxaliplatin inhibits OSCC by activating PARP1-mediated parthanatos through increasing the production of ROS.
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http://dx.doi.org/10.18632/aging.202386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906208PMC
January 2021

Antibacterial Properties of Bilayer Biomimetic Nano-ZnO for Dental Implants.

ACS Biomater Sci Eng 2020 04 12;6(4):1880-1886. Epub 2020 Mar 12.

The National Engineering Research Center for Bioengineering Drugs and the Technologies; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, China.

Dental implant surgery has a relatively high incidence of peri-implantitis. In this research, ZnO nanorods and ZnO nanospheres were synthesized by a hydrothermal method. ZnO nanorods first covered the surface of Ti or Ti-Zr, and ZnO nanospheres were then modified as the outermost layer. By these means a dual antibacterial effect could be realized by the rapid release of ZnO nanospheres and the sustained release of ZnO nanorods. Subsequent studies implied that this ZnO nanorods-nanospheres hierarchical structure (NRS) could be stably loaded on the surface of roughened Ti and Ti-Zr slices. The modified materials not only showed excellent antibacterial activities against both and but also showed low cellular cytotoxicity. This ZnO NRS structure is thus expected to be used as a general antimicrobial coating on the surface of Ti (Ti-Zr) in dental implant surgery.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01695DOI Listing
April 2020

Enhancing vigilance for cerebral air embolism after pneumonectomy: a case report.

BMC Pulm Med 2021 Jan 7;21(1):16. Epub 2021 Jan 7.

Department of Thoracic Surgery, Shenzhen Hospital, Southern Medical University, No.1333 Xinhu Road, Baoan District, Shenzhen, 518101, Guangdong, China.

Background: Vascular air embolism (VAE) is a rare but important complication that has not been paid enough attention to in the medical process such as surgery and anesthesia.

Case Presentation: We report for the first time that a 54-year-old male patient with central lung cancer developed severe complications of CAE after right pneumonectomy. After targeted first-aid measures such as assisted breathing, mannitol dehydration and antibiotic treatment, the patient gradually improved. The patient became conscious at discharge after 25 days of treatment but left limb was left with nerve injury symptoms.

Conclusion: We analyzed the possible causes of CAE in this case, and the findings from this report would be highly useful as a reference to clinicians.
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http://dx.doi.org/10.1186/s12890-020-01358-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788539PMC
January 2021

Peptide Probes with Aromatic Residues Tyr and Phe at the X Position Show High Specificity for Targeting Denatured Collagen in Tissues.

ACS Omega 2020 Dec 18;5(51):33075-33082. Epub 2020 Dec 18.

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

The construction of potent peptide probes for selectively detecting denatured collagen is crucial for a variety of widespread diseases. However, all of the denatured collagen-targeting peptide probes found till date primarily utilized the repetitive (Gly-X-Y) sequences with exclusively imino acids Pro and Hyp in the X and Y positions, which stabilized the triple helical conformation of the peptide probes, resulting in severe obstacles for their clinical applications. A novel series of peptide probes have been constructed by incorporating nonimino acids at the X position of the (GPO)GXO(GPO) sequence, while the X-site residue is varied as Tyr, Phe, Asp, and Ala, respectively. Peptide probes FAM-GYO and FAM-GFO containing aromatic residues Tyr and Phe at the X position showed similarly high binding affinity and tissue-staining efficacy as the well-established peptide probe FAM-GPO, while peptide probes FAM-GDO and FAM-GAO with the corresponding charged residue Asp and the hydrophobic residue Ala indicated much weaker binding affinity and tissue-staining capability. Furthermore, FAM-GYO and FAM-GFO could specifically detect denatured collagen in different types of mouse connective tissues and efficiently stain various human pathological tissues. We have revealed for the first time that the incorporation of nonimino acids, particularly aromatic residues at the X and Y positions of the repetitive (Gly-X-Y) sequences, may provide a convenient strategy to create novel robust collagen-targeting peptide probes, which have promising diagnostic applications in collagen-involved diseases.
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http://dx.doi.org/10.1021/acsomega.0c04684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774067PMC
December 2020

A t-SNE Based Classification Approach to Compositional Microbiome Data.

Front Genet 2020 14;11:620143. Epub 2020 Dec 14.

School of Statistics and Data Science, Nankai University, Tianjin, China.

As a data-driven dimensionality reduction and visualization tool, t-distributed stochastic neighborhood embedding (t-SNE) has been successfully applied to a variety of fields. In recent years, it has also received increasing attention for classification and regression analysis. This study presented a t-SNE based classification approach for compositional microbiome data, which enabled us to build classifiers and classify new samples in the reduced dimensional space produced by t-SNE. The Aitchison distance was employed to modify the conditional probabilities in t-SNE to account for the compositionality of microbiome data. To classify a new sample, its low-dimensional features were obtained as the weighted mean vector of its nearest neighbors in the training set. Using the low-dimensional features as input, three commonly used machine learning algorithms, logistic regression (LR), support vector machine (SVM), and decision tree (DT) were considered for classification tasks in this study. The proposed approach was applied to two disease-associated microbiome datasets, achieving better classification performance compared with the classifiers built in the original high-dimensional space. The analytic results also showed that t-SNE with Aitchison distance led to improvement of classification accuracy in both datasets. In conclusion, we have developed a t-SNE based classification approach that is suitable for compositional microbiome data and may also serve as a baseline for more complex classification models.
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http://dx.doi.org/10.3389/fgene.2020.620143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767995PMC
December 2020

Breastfeeding restored the gut microbiota in caesarean section infants and lowered the infection risk in early life.

BMC Pediatr 2020 11 25;20(1):532. Epub 2020 Nov 25.

Department of Pediatrics, The Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang, 050051, China.

Background: The initialization of the neonatal gut microbiota (GM) is affected by diverse factors and is associated with infant development and health outcomes.

Methods: In this study, we collected 207 faecal samples from 41 infants at 6 time points (1, 3, and 7 days and 1, 3, and 6 months after birth). The infants were assigned to four groups according to delivery mode (caesarean section (CS) or vaginal delivery (VD)) and feeding pattern (breastfeeding or formula milk).

Results: The meconium bacterial diversity was slightly higher in CS than in VD. Three GM patterns were identified, including Escherichia/Shigella-Streptococcus-dominated, Bifidobacterium-Escherichia/Shigella-dominated and Bifidobacterium-dominated patterns, and they gradually changed over time. In CS infants, Bifidobacterium was less abundant, and the delay in GM establishment could be partially restored by breastfeeding. The frequency of respiratory tract infection and diarrhoea consequently decreased.

Conclusion: This study fills some gaps in the understanding of the restoration of the GM in CS towards that in VD.
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http://dx.doi.org/10.1186/s12887-020-02433-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690020PMC
November 2020

Over 10-year follow-up outcomes and failure analysis of black diaphragm intraocular lens implantation in traumatic Aniridia.

Acta Ophthalmol 2020 Nov 16. Epub 2020 Nov 16.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, China.

Purpose: To determine the over 10-year follow-up outcomes and analyse the reason for failure in patients who underwent black diaphragm intraocular lens (IOL) implantation for the management of traumatic aniridia.

Methods: Fifty-three patients underwent black diaphragm IOL implantation for the treatment of traumatic aniridia from September 1998 to August 2007. 33 eyes of 33 patients were enrolled in our study, and the mean follow-up period was 185.6 ± 36.2 months (range: 126-247 months). The primary methods employed for assessment of the safety of the IOL and the causes of failure were the ultrasound biomicroscopy (UBM) and observation during reoperation.

Results: In the current study, 13 patients (39.4%) who underwent IOL implantation by means of trans-scleral fixation were observed to maintain a transparent cornea and normal intraocular pressure. 20 patients (60.6%) developed corneal endothelial decompensation, and 14 patients (42.4%) developed secondary glaucoma. 27 patients were examined by means of UBM or reoperation and the position of black diaphragm IOL in 18 patients was observed to be abnormal; the IOL had shifted forward and directly contacted with the anterior chamber and even the corneal endothelium.

Conclusion: The forward displacement of black diaphragm IOL is a direct cause of treatment failure. Although the implantation of black diaphragm IOL is an effective method of management of traumatic aniridia, the treatment should be guarded and performed in an appropriate manner. Patients should be informed regarding the need for life-long follow-up, so that the complications can be identified in a timely manner.
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http://dx.doi.org/10.1111/aos.14667DOI Listing
November 2020

The epidemic of erythromycin-resistant with limited genome variation associated with pertussis resurgence in China.

Expert Rev Vaccines 2020 11 21;19(11):1093-1099. Epub 2020 Oct 21.

Beijing Pediatric Research Institute of Beijing Children's Hospital Affiliated to Capital Medical University , Beijing, China.

Background: The resurgence of infections leading to whooping cough is a concern in many parts of the world. The number of pertussis cases in China has increased significantly since 2013.

Research Design And Methods: In this study, whole-genome sequencing analysis was performed for 388 strains isolated in China from the 1970s to 2018, combining 594 published strains from around the world.

Results: This study revealed that lineage V diverged about 50 years ago in China, while lineage IV is dominant in the other countries. It also revealed that the erythromycin-resistant sub-lineages Va, Vb, and Vc with limited genomic variation emerged 11 ~ 12 years ago. These three sub-lineages were identified after the co-purified acellular vaccines (cp-ACVs) completely replaced the previous whole cell vaccines (WCVs) after the national immunization program of 2012. It suggests that the cp-ACVs cannot induce immunity that is potent enough to restrict the spread of the lineage V, antibiotic abuse further favors the spread of this lineage in China.

Conclusions: These findings demand a reassessment of the immunization strategy and development of new vaccines in China to stop the resurgence and drug resistance of .
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http://dx.doi.org/10.1080/14760584.2020.1831916DOI Listing
November 2020

Development of Magnet-Driven and Image-Guided Degradable Microrobots for the Precise Delivery of Engineered Stem Cells for Cancer Therapy.

Small 2020 10 20;16(41):e1906908. Epub 2020 Sep 20.

Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, 999077, China.

Precise delivery of therapeutic cells to the desired site in vivo is an emerging and promising cellular therapy in precision medicine. This paper presents the development of a magnet-driven and image-guided degradable microrobot that can precisely deliver engineered stem cells for orthotopic liver tumor treatment. The microrobot employs a burr-like porous sphere structure and is made with a synthesized composite to fulfill degradability, mechanical strength, and magnetic actuation capability simultaneously. The cells can be spontaneously released from the microrobots on the basis of the optimized microrobot structure. The microrobot is actuated by a gradient magnetic field and guided by a unique photoacoustic imaging technology. In preclinical experiments on nude mice, microrobots carrying cells are injected via the portal vein and the released cells from the microrobots can inhibit the tumor growth greatly. This paper reveals for the first time of using degradable microrobots for precise delivery of therapeutic cells in vascular tissue and demonstrates its therapeutic effect in preclinical test.
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http://dx.doi.org/10.1002/smll.201906908DOI Listing
October 2020

Highly specific recognition of denatured collagen by fluorescent peptide probes with the repetitive Gly-Pro-Pro and Gly-Hyp-Hyp sequences.

J Mater Chem B 2020 11;8(44):10093-10100

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

Denatured collagen is a key biomarker for various critical diseases such as cancer. Peptide probes with the repetitive (Gly-Pro-Hyp)n sequences have recently been found to selectively target denatured collagen; however, thermal or UV pretreatment is required to drive the peptides into the monomer conformation, which poses a substantial challenge for clinical applications. We herein construct two peptide probes, FAM-GOO and FAM-GPP, consisting of the repetitive (Gly-Hyp-Hyp)8 and (Gly-Pro-Pro)8 sequences, respectively. The CD, fluorescence and colorimetric studies have consistently revealed that FAM-GOO showed strong capability of forming the triple helical structure, while FAM-GPP pronouncedly displayed the single stranded conformation at temperatures as low as 4 °C. The binding experiments have indicated that both peptide probes could recognize denatured collagen with high specificity, and FAM-GPP remarkably did not need the preheating treatment. The tissue staining results have shown that preheated FAM-GOO and unheated FAM-GPP could target denatured collagen in a wide variety of rat frozen and human FFPE tissue sections. Compared with antibodies specific for a certain type of collagen, both FAM-GOO and FAM-GPP act as broad-spectrum probes for the selective detection of denatured collagen of different types and from different species. Importantly, FAM-GPP possessed the unique capability of maintaining the monomer conformation by itself, thus avoiding the potential risks of the thermal or UV pretreatment. This novel peptide probe provides a handy and versatile biosensor for specifically targeting denatured collagen, which has attractive potential in the diagnosis and therapeutics of collagen-involved diseases.
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http://dx.doi.org/10.1039/d0tb01691hDOI Listing
November 2020

Characterization of the variable merozoite surface antigen (VMSA) gene family of Babesia orientalis.

Parasitol Res 2020 Nov 15;119(11):3639-3648. Epub 2020 Sep 15.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Due to its wide presence in apicomplexan parasites as well as high polymorphism and antigenic diversity, the variable merozoite surface antigen (VMSA) family in Babesia sp. has attracted increasing attention of researchers. Here, all the reported VMSA genes of Babesia spp. were obtained from GenBank, and multiple alignments were performed by using conserved regions to blast the Babesia orientalis genome database (unpublished data). Five MSA genes (named MSA-2a1, MSA-2a2, MSA-2c1, MSA-1, and MSA-2c2, respectively) were identified, sequenced, and cloned from B. orientalis, which were shown to encode proteins with open reading frames ranging in size from 266 (MSA-2c1) to 317 (MSA-1) amino acids. All the five proteins contain an MSA-2c superfamily conserved domain, with an identical signal peptide and glycosyl phosphatidyl inositol (GPI)-anchor for each of them. The five proteins were also predicted to contain B cell epitopes, with only three for BoMSA-2c1, the smallest protein in the BoVMSA family, while at least six for each of the others. Notably, BoMSA-2a1 has 2 identical copies, a specific phenomenon only present in B. orientalis. This research has determined the MSA genes of B. orientalis and provides a genetic basis for further research of functional genes in B. orientalis.
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http://dx.doi.org/10.1007/s00436-020-06877-zDOI Listing
November 2020

Is antibiotics prescription needed in infants with topical corticosteroids treatment for moderate-to-severe atopic dermatitis?

Dermatol Ther 2020 11 11;33(6):e14215. Epub 2020 Sep 11.

Department of Dermatology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

The cutaneous microbiota responses to skin health as well as atopic dermatitis. To reveal the microbiota effect in atopic dermatitis children under therapy with topical corticosteroids and antibiotics. 59 atopic dermatitis patients were randomized to two treatment groups (by corticosteroids or combination therapy) in Beijing Children's Hospital. The lesion microbial samples were collected for 16S rDNA sequencing and bioinformatics analysis. After treatment, 57 patients recovered significantly. Though topical antibiotics application blocked the restoration of commensal Streptococcus, no remarkable differences of cutaneous microbiota were identified between the two groups along the treatment. In subject 1081, who received the combination therapy, the Streptococcus and Pseudomonas as well as Chryseobacterium increased dramatically. On the contrary, the Staphylococcus aureus decreased sharply in subject 1107 with topical corticosteroids treatment Our preliminary study suggested the necessity to consider cutaneous microbiota profile when prescribing antibiotics.
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http://dx.doi.org/10.1111/dth.14215DOI Listing
November 2020

Genotyping and identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from free-range Tibetan yellow cattle and cattle-yak in Tibet, China.

Acta Trop 2020 Dec 18;212:105671. Epub 2020 Aug 18.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan Province, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, No. 15 Longzihu University Area, Zhengdong New District, Zhengzhou 450002, China. Electronic address:

To assess the prevalence and molecular characteristics of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in natural grazing local breed cattle, 513 fecal samples from Tibetan yellow cattle and cattle-yaks were tested for these pathogens. Cryptosporidium, G. duodenalis and E. bieneusi in Tibetan yellow cattle prevalence were 0.7% (3/442), 3.8% (17/442) and 2.5% (11/442), respectively. Cryptosporidium bovis (n = 3), G. duodenalis assemblages A (n = 2) and E (n = 15) were identified based on sequence analysis. Among three E. bieneusi genotypes, I (n = 7), EbpC (n = 2) and CHC8 (n = 2) detected, EbpC was classified into Group 1, which has a significant zoonotic importance, whereas genotypes I and CHC8 belonged to Group 2. None of these pathogens was identified in cattle-yaks. The presence of zoonotic C. bovis, assemblage A and EbpC indicates Tibetan yellow cattle may be a potential spread source of intestinal pathogens with a zoonotic transmission risk. The relationships between natural free-range mode and the prevalence or genetic diversity of these pathogens need be confirmed in further studies.
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http://dx.doi.org/10.1016/j.actatropica.2020.105671DOI Listing
December 2020

Corrigendum: Alterations of Gut Microbiota in Cholestatic Infants and Their Correlation With Hepatic Function.

Front Microbiol 2020 24;11:1599. Epub 2020 Jul 24.

Department of Pediatrics, The Third Hospital of Hebei Medical University, Shijiazhuang, China.

[This corrects the article DOI: 10.3389/fmicb.2018.02682.].
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http://dx.doi.org/10.3389/fmicb.2020.01599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393271PMC
July 2020

Scleral defect repair using decellularized porcine sclera in a rabbit model.

Xenotransplantation 2020 11 29;27(6):e12633. Epub 2020 Jul 29.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China.

Background: The sclera is one of the most commonly used repair materials in ophthalmic plastic surgery and is often used for supporting, wrapping, filling, and pressing during surgery. Although the sclera plays an irreplaceable role in ophthalmology applications, there are many restrictive factors, such as high costs and limited sources. Here, we report the use of a decellularized porcine sclera (DPS) for scleral reconstruction in rabbit models.

Methods: The DPS generated by a hybrid decellularization protocol was characterized in respect of histological observation, DNA, α-gal, GAG, and collagen content. The mechanical properties were evaluated by uniaxial tensile testing. LIVE/DEAD and Cell Counting Kit (CCK)-8 assays were performed to assess its in vitro cytocompatibility and cytotoxicity. In vivo biocompatibility and biointegration of the DPS for repairing scleral defect in rabbit were measured by slit-lamp and histological analyses. Immunohistochemical (IHC) staining was used to detect the expression of CD4, CD8, CD45, CD68, and vimentin.

Results: Through decellularization, the major xenoantigen DNA and α-gal are efficiently removed while abundant matrix components and mechanical properties are well preserved in the DPS. Extracts of the DPS and DPS samples had no inhibitory effects on the proliferation of HFSFs. Moreover, there was no sign that an immune reaction occurred in or around the transplanted DPS grafts within 28 days of animal implantation.

Conclusion: The decellularization strategy we developed is feasible and effective. The prepared DPS holds great potential for the repair of scleral injury.
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http://dx.doi.org/10.1111/xen.12633DOI Listing
November 2020