Publications by authors named "Dong-sheng Wang"

151 Publications

Inhibitory effect of Aphidicolin - a tetracyclic diterpene - on the proliferation and apoptotic induction in human cervical cancer (HeLa) cells.

J BUON 2021 Jan-Feb;26(1):291

Department of Obstetrics and Gynecology, Shandong University Hospital, Jinan 250100, China.

Retraction of: 'Inhibitory effect of Aphidicolin - a tetracyclic diterpene - on the proliferation and apoptotic induction in human cervical cancer (HeLa) cells', by En-Yan Yu, Rui-Yan Zhao, Dong-Sheng Wang, JBUON 2015;20(6):1480-1486; PMID:26854444. Following the publication of the above article, readers drew to our attention that part of the data was unreliable. The authors were requested to provide the raw data to prove the originality, but were unable to do so. After an investigation, the Editors of JBUON decided to retract this article. We thank the readers for bringing this matter to our attention. We apologize for any inconvenience it may cause.
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March 2021

Aggravated ulcerative colitis caused by intestinal Metrnl deficiency is associated with reduced autophagy in epithelial cells.

Acta Pharmacol Sin 2020 Jun 16;41(6):763-770. Epub 2020 Jan 16.

Department of Pharmacology, Second Military Medical University/Naval Medical University, Shanghai, 200433, China.

Metrnl is a newly identified secreted protein highly expressed in the intestinal epithelium. This study aimed to explore the role and mechanism of intestinal epithelial Metrnl in ulcerative colitis. Metrnl (intestinal epithelial cell-specific Metrnl knockout) mice did not display any phenotypes of colitis under basal conditions. However, under administration of 3% dextran sodium sulfate (DSS) drinking water, colitis was more severe in Metrnl mice than in WT mice, as indicated by comparisons of body weight loss, the presence of occult or gross blood per rectum, stool consistency, shrinkage in the colon, intestinal damage, and serum levels of inflammatory factors. DSS-induced colitis activated autophagy in the colon. This activation was partially inhibited by intestinal epithelial Metrnl deficiency, as indicated by a decrease in Beclin-1 and LC3-II/I and an increase in p62 in DSS-treated Metrnl mice compared with WT mice. These phenomena were further confirmed by observation of autophagosomes and immunofluorescence staining for LC3 in epithelial cells. The autophagy-related AMPK-mTOR-p70S6K pathway was also activated in DSS-induced colitis, and this pathway was partially blocked by intestinal epithelial Metrnl deficiency, as indicated by a decrease in AMPK phosphorylation and an increase in mTOR and p70S6K phosphorylation in DSS-treated Metrnl mice compared with WT mice. Therefore, Metrnl deficiency deteriorated ulcerative colitis at least partially through inhibition of autophagy via the AMPK-mTOR-p70S6K pathway, suggesting that Metrnl is a therapeutic target for ulcerative colitis.
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http://dx.doi.org/10.1038/s41401-019-0343-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471395PMC
June 2020

Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist sp.

Biomolecules 2019 11 4;9(11). Epub 2019 Nov 4.

Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.

In the current study, corn steep liquor (CSL) is evaluated as an ideal raw agro-material for efficient lipid and docosahexaenoic acid DHA production by sp. Low CSL level in medium (nitrogen deficiency) stimulated the biosynthesis of lipids and DHA while inhibiting cellular growth. The transcriptomic profiles of the sp. cells are analyzed and compared when cultured under high (H group), normal (N group), and low (L group) levels of CSL in the medium. The discriminated transcriptomic profiles from the three groups indicates that changes in CSL level in medium result in a global change in transcriptome of sp. The overall de novo assembly of cDNA sequence data generated 61,163 unigenes, and 18,129 of them were annotated in at least one database. A total of 5105 differently expressed (DE) genes were found in the N group versus the H group, with 2218 downregulated and 2887 upregulated. A total of 3625 DE genes were found in the N group versus the L group, with 1904 downregulated and 1721 upregulated. The analysis and categorization of the DE genes indicates that the regulation mechanism of CSL involved in the perception and transduction of the limited nitrogen signal, the interactions between the transcription factors (TFs) and multiple downstream genes, and the variations in downstream genes and metabolites, in sequence, are illuminated for the first time in the current study.
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http://dx.doi.org/10.3390/biom9110695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920895PMC
November 2019

Diagnostic value of gamma-glutamyltransferase/aspartate aminotransferase ratio, protein induced by vitamin K absence or antagonist II, and alpha-fetoprotein in hepatitis B virus-related hepatocellular carcinoma.

World J Gastroenterol 2019 Sep;25(36):5515-5529

Department of Laboratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China.

Background: Researchers have investigated the diagnostic value of protein induced by vitamin K absence or antagonist II (PIVKA-II) and alpha-fetoprotein (AFP) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), and obtained abundant clinical diagnostic data. However, PIVKA-II and AFP have unsatisfactory specificity and sensitivity in the diagnosis of early-stage HBV-related HCC. Gamma-glutamyltransferase (γ-GT) and aspartate aminotransferase (AST) are common biomarkers for evaluating liver function, and we hypothesized that the γ-GT/AST ratio in combination with PIVKA-II and AFP would improve the diagnosis of early-stage HBV-related HCC.

Aim: To evaluate the diagnostic value of γ-GT/AST ratio alone or in combination with PIVKA-II and AFP in HBV-related HCC.

Methods: Serum levels of γ-GT, AST, PIVKA-II, and AFP were detected and analysed in 176 patients with HBV-related HCC and in 359 patients with chronic hepatitis B. According to tumour size and serum level of HBV DNA, HBV-related HCC patients were divided into the following categories: Early-stage HCC patients, HCC patients, HBV DNA positive (HBV DNA+) HCC patients, and HBV DNA negative (HBV DNA-) HCC patients. Receiver-operating characteristic (ROC) curves were used to analyse and compare the diagnostic value of the single and combined detection of various biomarkers in different types of HBV-related HCC.

Results: Tumour size was positively correlated with serum levels of PIVKA-II and AFP in HCC patients ( = 0.529, < 0.001 and = 0.270, < 0.001, respectively), but there was no correlation between tumour size and the γ-GT/AST ratio ( = 0.073, = 0.336). The areas under the receiver-operating characteristic curves (AUROCs) of the γ-GT/AST ratio in early-stage HCC patients, HBV DNA+ HCC patients and HBV DNA- HCC patients were not significantly different from that in the total HCC patients (0.754, 0.802, and 0.705 0.779, respectively; > 0.05). When PIVKA-II was combined with the γ-GT/AST ratio in the diagnosis of early-stage HCC, HCC, and HBV DNA+ HCC, the AUROCs of PIVKA-II increased, with values of 0.857 0.835, 0.925 0.913, and 0.958 0.954, respectively. When AFP was combined with the γ-GT/AST ratio in the diagnosis of early-stage HCC, HCC, HBV DNA+ HCC, and HBV DNA- HCC, the AUROCs of AFP increased, with values of 0.757 0.621, 0.837 0.744, 0.868 0.757, and 0.840 0.828, respectively.

Conclusion: The γ-GT/AST ratio may be better than PIVKA-II and AFP in the diagnosis of early-stage HBV-related HCC, and its combination with PIVKA-II and AFP can improve the diagnostic value for HBV-related HCC.
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http://dx.doi.org/10.3748/wjg.v25.i36.5515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6767977PMC
September 2019

NMR Metabolomics and Random Forests Models to Identify Potential Plasma Biomarkers of Blood Stasis Syndrome With Coronary Heart Disease Patients.

Front Physiol 2019 4;10:1109. Epub 2019 Sep 4.

Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Coronary heart disease (CHD) remains highly prevalent and is one of the largest causes of death worldwide. Blood stasis syndrome (BSS) is the main syndrome associated with CHD. However, the underlying biological basis of BSS with CHD is not yet been fully understood.

Materials And Methods: We proposed a metabolomics method based on H-NMR and random forest (RF) models to elucidate the underlying biological basis of BSS with CHD. Firstly, 58 cases of CHD patients, including 27 BSS and 31 phlegm syndrome (PS), and 26 volunteers were recruited from Xiangya Hospital affiliated to Central South University. A 1 mL venous blood sample was collected for NMR analysis. Secondly, principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA) and RF was applied to observe the classification of each group, respectively. Finally, RF and multidimensional scaling (MDS) were utilized to discover the plasma potential biomarkers in CHD patients and CHD-BSS patients.

Results: The models constructed by RF could visually discriminate BSS from PS in CHD patients. Simultaneously, we obtained 12 characteristic metabolites, including lysine, glutamine, taurine, tyrosine, phenylalanine, histidine, lipid, citrate, choline, lactate, α-glucose, β-glucose related to the CHD patients, and Choline, β-glucose, α-glucose and tyrosine were considered as potential biomarkers of CHD-BSS.

Conclusion: The combining of H-NMR profiling with RF models was a useful approach to analyze complex metabolomics data (should be deleted). Choline, β-glucose, α-glucose and tyrosine were considered as potential biomarkers of CHD-BSS.
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http://dx.doi.org/10.3389/fphys.2019.01109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738169PMC
September 2019

Phosphate-solubilizing bacteria will not significantly remobilize soil cadmium remediated by weathered coal.

Environ Sci Pollut Res Int 2019 Oct 6;26(28):29003-29011. Epub 2019 Aug 6.

School of Environment, Key Laboratory of Environmental Pollution and Health of Guangdong Province, Jinan University, Guangzhou, 510632, China.

Cadmium (Cd) re-mobilize by phosphate-solubilizing bacteria (PSB) from immobilization contaminated soil has drawn great attention due to its serious threat to human health through food chain. However, Cd binding with weathered coal (WC), an effective Cd immobilization material, will be re-mobilized by PSB or not is still unclear. In this study, the soil and sand pots with Cd were respectively mixed with the weight fractions of 0‰, 2‰, and 3‰ WC, inoculated with or without PSB, and planted with Amaranthus mangostanus L. The experimental results indicated that: (i) Cd in soil was transformed into organic fraction with WC, which has been led to the Cd accumulation concentrations in roots and shoots reduced by 38.8% and 20.5%, respectively; (ii) PSB could promote the concentration of exchangeable-Cd fraction and soil Cd uptake by amaranth in all treatments; and (iii) WC application in sand pot respectively reduced the Cd accumulation by 47.5% in roots and 24.1% in shoots, but PSB inoculation showed no significant effect on Cd accumulation in plants under WC application. SEM, zeta potential, and FT-IR results showed that PSB inoculation after Cd immobilized by WC had no influence on the microstructure, amount of negative charge, type, and content of functional groups in WC, indicating that organic fraction Cd in WC was not re-mobilized by PSB. Therefore, the application of WC in contaminated soil was conducive to transforming Cd in organic-bound forms and intensifying Cd immobilization effects.
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http://dx.doi.org/10.1007/s11356-019-06142-6DOI Listing
October 2019

Influence of irrigation with microalgae-treated biogas slurry on agronomic trait, nutritional quality, oxidation resistance, and nitrate and heavy metal residues in Chinese cabbage.

J Environ Manage 2019 Aug 30;244:453-461. Epub 2019 May 30.

Guangdong Institute of Microbiology, Guangzhou, 510632, China. Electronic address:

Biogas slurry (BS) is a main byproduct of biogas production that is commonly used for agricultural irrigation because of its abundant nutrients and microelements. However, direct application of BS may cause quality decline and nitrate and heavy metal accumulation in crops. To address this issue, a microalgae culture experiment and an irrigation experiment were performed to evaluate the removal efficiencies of nutrients and heavy metals from diluted BS by microalgae Scenedesmus sp. and to investigate the effects of irrigation with microalgae-treated BS (MBS-25, MBS-50, MBS-75, and MBS-100) on nutritional quality, oxidation resistance, and nitrate and heavy metal residues in Chinese cabbage. After 8 days of continuous culture, a ratio of 1/1 for BS/tap water mixture (BS-50) was the optimal proportion for microalgal growth (3.73 g dry cell L) and efficient removal of total nitrogen (86.1%), total phosphorus (94.3%), COD (87.5%), Cr (50%), Pb (60.7%), and Cd (59.7%). The pH in MBS-50 medium recovered to the highest level in a shorter period of time and accelerated the gas stripping of ammonia nitrogen and the formation of insoluble phosphate and metals, which partly contributed to the high removal efficiencies. MBS irrigation significantly promoted crop growth; improved nutritional quality, edible taste, and oxidation resistance; and reduced nitrate and heavy metal residues in Chinese cabbage at a large scale. Therefore, microalgae culture was beneficial to reduce negative impacts of BS irrigation in crop growth and agricultural product safety. This study may provide a theoretical basis for the safe utilization of BS waste in agricultural irrigation.
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http://dx.doi.org/10.1016/j.jenvman.2019.04.058DOI Listing
August 2019

Technology of percutaneous cannulated screw implantation using screw view model of navigation in Garden type I of femoral neck fracture: A case report.

Medicine (Baltimore) 2019 May;98(21):e15591

Department of Orthopaedics.

Rationale: The aim of the present study was to assess the efficacy and safety of percutaneous cannulated screw (PCS) implantation assisted by screw view model of navigation (SVMN) to treat femoral neck fracture (FNF).

Patient Concerns: A 42-year-old male patient suffered from a high falling injury, causing pain, swelling, deformity, and limited mobility on his right hip.

Diagnoses: He was diagnosed with Garden type I of FNF.

Interventions: PCS implantation assisted by SVMN was used to treat fracture of femoral neck in this patient.

Outcomes: The follow up lasted for 48 months. A total of 3 screws were inserted into femoral neck, all exhibiting excellent position. The mean screw deviation was 0.43° and 5.73° of femoral neck-shaft and anteversion angle, respectively. The guide wire drilling attempt of each screw was one-time. The fluoroscopic time lasted 6.3 minutes, the Harris hip scores improved from 67 to 88, and the blood loss was 35 mL. It took 11.7 minutes for designing the screws, 13.9 minutes for implanting the guide wires, and 37.3 minutes for placing the screws. No clinical complications were found during 48-month follow-up visit, including head penetration, implant failure, fracture nonunion, and femoral head osteonecrosis.

Lessons: The study revealed that SVMN is conducive to the PCS insertion for FNF. Our lesson is that the FNF must be well reduction before SVMN assisted PCS placement.
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http://dx.doi.org/10.1097/MD.0000000000015591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571405PMC
May 2019

[VOCs and Odors Control and Development in Pharmaceutical Fermentation Industry].

Huan Jing Ke Xue 2019 Apr;40(4):1990-1998

University of Chinese Academy of Sciences, Beijing 100049, China.

Volatile organic compounds (VOCs) and odors, which pose potential hazards to human health and the ecosystem, are two of the most important pollutants emitted from the pharmaceutical fermentation industry. Currently, basic research on the characteristics of the pollution and effective prevention technology for VOCs and odors emitted from the pharmaceutical fermentation industry are limited. Specifically, the pharmaceutical fermentation industry lacks adequate theoretical guidance on the supervision and control of VOCs and odors, and some companies even face relocations. Using the pharmaceutical fermentation industry as the study object, the pollution characteristics of VOCs and odors emitted from different production workshops, sewage treatment stations, and the disposal of pharmaceutical residues were assessed. Based on the studies above, the progress of research into representative control technologies were also reviewed systematically. For VOCs and odors control in the pharmaceutical fermentation industry, four suggestions for future research were proposed:① The production processes should be optimized, and the discharge of pollution should be reduced throughout the entire processes; ② Basic research should be carried out on the pollution characteristics of the VOCs and odors emitted from the pharmaceutical fermentation industry, and a rapid and effective method to trace the sources of VOCs and odors should be established; ③ A comprehensive evaluation of control technologies should be conducted, taking cost and efficiency into account; ④ Emission standards and technical orders for VOCs and odors in the pharmaceutical fermentation industry should be formulated and implemented immediately.
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http://dx.doi.org/10.13227/j.hjkx.201807107DOI Listing
April 2019

High-Throughput Biochemical Fingerprinting of Oleaginous sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions.

Molecules 2019 Apr 22;24(8). Epub 2019 Apr 22.

Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.

The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist sp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109 sp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the two sp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginous sp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.
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http://dx.doi.org/10.3390/molecules24081593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514702PMC
April 2019

Computationally Universal Phase of Quantum Matter.

Phys Rev Lett 2019 Mar;122(9):090501

Institut für Theoretische Physik, Universität Innsbruck, Technikerstraße 21a, A-6020 Innsbruck, Austria.

We provide the first example of a symmetry protected quantum phase that has universal computational power. This two-dimensional phase is protected by one-dimensional linelike symmetries that can be understood in terms of the local symmetries of a tensor network. These local symmetries imply that every ground state in the phase is a universal resource for measurement-based quantum computation.
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http://dx.doi.org/10.1103/PhysRevLett.122.090501DOI Listing
March 2019

Transcriptomic Mechanism of the Phytohormone 6-Benzylaminopurine (6-BAP) Stimulating Lipid and DHA Synthesis in Aurantiochytrium sp.

J Agric Food Chem 2019 May 2;67(19):5560-5570. Epub 2019 Apr 2.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering , Zhejiang University of Technology , No. 18, Chaowang Road , Hangzhou 310014 , People's Republic of China.

The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7% and 55.3%, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53871 unigenes, and 15902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes, and metabolic changes, respectively, was put forward for the first time in the present study. This research illuminates the transcriptomic mechanism of phytohormone stimulation of lipid and DHA production in an oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.
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http://dx.doi.org/10.1021/acs.jafc.8b07117DOI Listing
May 2019

Expression and methylation status of LAMA2 are associated with the invasiveness of nonfunctioning PitNET.

Ther Adv Endocrinol Metab 2019 29;10:2042018818821296. Epub 2019 Jan 29.

Department of Neurosurgery, Shenzhen People's Hospital, Shenzhen, China; The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). In the current study, we investigated the relationship between LAMA2 promoter methylation status and the invasiveness of clinically nonfunctioning pituitary adenomas (PitNETs). Specimens from patients with nonfunctioning PitNET were classified into three groups according to preoperative computed tomography (CT)/magnetic resonance imaging findings: a normal group ( = 6), non-invasive group ( = 11) and invasive group ( = 6). LAMA2 expression was assessed using quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting, and the methylation status of the LAMA2 promoter region was observed using sodium bisulfite sequencing. Furthermore, 5-aza-2-deoxycytidine was used to explore the relationship between decreased LAMA expression and methylation in PitNET cells. According to the RT-qPCR and western blotting results, LAMA2 expression was downregulated in invasive PitNET, while the methylation of the LAMA2 promoter was increased. Methylation of the LAMA2 promoter decreased the expression of LAMA2. Thus, changes in LAMA2 expression due to promoter methylation were inversely correlated with the invasiveness of PitNET and the protein functions as a tumor suppressor. In addition, overexpression and demethylation of LAMA2 suppressed the invasion of PitNET cells, partially by exerting effects on the PTEN-PI3K/AKT signaling pathway and matrix metalloproteinase-9 (MMP-9). Furthermore, a xenograft model was also generated, and LAMA2 overexpression significantly suppressed tumor growth . Thus, LAMA2 expression and methylation patterns might be used as biomarkers to predict the prognosis of patients with PitNET.
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http://dx.doi.org/10.1177/2042018818821296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351710PMC
January 2019

Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion.

Oncol Lett 2019 Feb 10;17(2):2057-2062. Epub 2018 Dec 10.

Department of General Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China.

The biological features of pancreatic cancer and the associated hypoxic environment around the cancer cells often lead to resistance to radiotherapy and chemotherapy. The present study was performed in order to explore the effect pancreatic stellate cells (PSCs) have on the proliferation of pancreatic cancer cells. In the present study, PSCs from human pancreatic cancer tissues were isolated, and the PSCs markers α-smooth muscle actin and desmin were overexpressed in the cytoplasm of PSCs. An MTT assay revealed that PSCs promoted the viability of pancreatic cancer cells. However, the viability of pancreatic cancer cells promoted by PSCs was partially blocked by SB525334. Cellular invasion analysis demonstrated that PSCs promoted the invasion ability of pancreatic cancer cells. An apoptosis assay indicated that PSCs decreased the level of apoptosis induced by gemcitabine. experiments consisting of mice bearing MIA-PaCa-2 and PSCs demonstrated an increase in the rate of tumor growth compared with MIA-PaCA-2 alone, whereas SB525334 may delay the tumor progression induced by PSCs. The present findings indicated that PSCs promoted the viability and invasion of pancreatic cancer cells, and decreased the apoptosis of pancreatic cancer cells induced by gemcitabine.
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http://dx.doi.org/10.3892/ol.2018.9816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341873PMC
February 2019

[Influence of the Coagulation Mechanism on the Coagulation Performances Using New Composite Coagulants: Role of the Raw Water Characteristics].

Huan Jing Ke Xue 2019 Jan;40(1):263-272

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

To improve the coagulation performances, new composite coagulants were used to treat different water samples. The results indicate that Ca has no significant effects on the removal efficiency for turbidity in the kaolin system. The residual aluminum decreased from 0.15 mg·L to 0.10 mg·L (AlCl was used as coagulant and the coagulant dosage was 0.10 mmol·L). The presence of Ca led to the decrease of the amount of negative charges in the HA system and the residual aluminum decreased due to the decrease of the complexation between the HA molecules and Al-based coagulants. When the raw water contained BSA molecules and the coagulant dosage was 0.16 mmol·L, the flocs formed by PACl with Ca were larger (~50 μm) than the flocs generated by PACl and the settleability also improved. Under alkaline conditions (pH=8.5), the DOC concentration decreased after coagulation process by~0.2-0.6 mg·L and the residual aluminum decreased by~0.4-0.7 mg·L using composite coagulants. Under acidic conditions (pH=5.5), the concentrations of DOC and residual aluminum did not significantly differ.
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http://dx.doi.org/10.13227/j.hjkx.201802045DOI Listing
January 2019

Accuracy and safety of percutaneous periacetabular screw insertion using screw view model of navigation in acetabular fracture: A case report.

Medicine (Baltimore) 2018 Dec;97(49):e13316

Department of Orthopaedics.

Rationale: The purpose of this study was to estimate the efficacy and safety of percutaneous periacetabular screw (PPS) insertion assisted by screw view model of navigation (SVMN) to treat fracture of acetabulum.

Patient Concerns: A 61-year-old male patient was injured in a motorcycle accident, which caused pain, swelling, deformity and limited mobility on his right hip.

Diagnoses: He was diagnosed with fracture of acetabulum.

Interventions: We used PPS insertion assisted by SVMN to treat fracture of acetabulum in this patient.

Outcomes: The follow up lasted 24 months. Totally 2 screws were inserted into anterior and posterior column of acetabulum respectively and both of them displayed grade 0. Compared with the preoperative gap and step of fracture displacement, the postoperative ones were significantly reduced. It took 11.7 minutes for designing the screws, 6.7 minutes for implanting the guide wire, and 45.5 minutes for placing the screws. Intraoperative blood loss was 29 mL and total fluoroscopic time was 4.1 minutes. No clinical complications such as nerve vascular injury, infection and screw loosening were found after the operation.

Lessons: The study indicated that SVMN is favorable to the PPS insertion for acetabular fracture. Our lesson is that the relative position between the acetabular and the patient tracker must be static to ensure the accuracy of the entire system throughout the operation.
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http://dx.doi.org/10.1097/MD.0000000000013316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310583PMC
December 2018

Copper Sulfide-Based Plasmonic Photothermal Membrane for High-Efficiency Solar Vapor Generation.

ACS Appl Mater Interfaces 2018 Oct 2;10(41):35154-35163. Epub 2018 Oct 2.

Department of Chemistry , University of Missouri-Kansas City , Kansas City , Missouri 64110 , United States.

Solar vapor generation has attracted tremendous attention as one of the most efficient ways of utilizing solar energy. It is highly desirable to develop low-cost, eco-friendly, and high-efficiency solar absorbers for practical applications of solar vapor generation. Herein, a three-dimensional plasmonic covellite CuS hierarchical nanostructure has been synthesized as the light-absorbing material via a facile one-pot hydrothermal method for structurally integrated solar absorbers with microporous poly(vinylidene fluoride) membrane (PVDFM) as the supporting material. A broadband and highly efficient light absorption has been achieved in the wavelength of 300-2500 nm, along with high water evaporation efficiencies of 90.4 ± 1.1 and 93.3 ± 2.0% under 1 and 4 sun irradiation, respectively. Meanwhile, stable performance has been demonstrated for over 20 consecutive runs without much performance degradation. To the best of our knowledge, this is the highest performance among the copper sulfide-based solar absorbers. With the additional features of low-cost and convenient fabrication, this plasmonic solar absorber exhibits a tremendous potential for practical solar vapor generation.
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http://dx.doi.org/10.1021/acsami.8b11786DOI Listing
October 2018

[Effect of Cr(Ⅵ) on Coagulation Process of Different Coagulants].

Huan Jing Ke Xue 2018 Aug;39(8):3704-3712

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

In this study, the effect of Cr(Ⅵ) in industrial wastewater on the coagulation of different aluminum forms under the influence of different particle concentrations was investigated. The coagulation efficiency was determined by examining the removal rate of turbidity, residual aluminum, and residual chromium and by using the residual pH value, zeta potential, and floc properties of the coagulation to reveal the coagulation mechanism of Cr(Ⅵ) in water under different turbidities. The experimental results show that under low turbidity conditions, Cr(Ⅵ) greatly influences the coagulation process of highly polymerized Al, yet has no obvious effect on oligomeric Al. Under high turbidity conditions, particulate matter will adsorb part of the Cr(Ⅵ) in high turbidity water, thus reducing its interaction with Al. The main role Al plays in coagulation is charge neutralization. It plays the same role in the stability of the particles and floc regeneration. The main role of Al formed by hydrolysis of Al is bridging effects and sweep flocculation, which plays an important role in floc production and strength factor. At the same time, the existence of Cr(Ⅵ) enhances the strength factor of Al flocs, but the consumption of some of the positive charge will lead to a reduction in the floc recovery factor.
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http://dx.doi.org/10.13227/j.hjkx.201710034DOI Listing
August 2018

[Effects of Algal Morphology and Al Species Distribution on the Coagulation-Ultrafiltration Process].

Huan Jing Ke Xue 2017 Aug;38(8):3281-3289

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

In order to ensure drinking water quality, three different Al-based coagulants [Al(SO)(AS), Al, Al] were used to treat water laden with different algae [(cyanobacteria), (green algae), (diatoms)]. Floc size, strength factor, and recovery factor under different conditions were measured to investigate the mechanisms in the coagulation-ultrafiltration process. The results indicated that the main mechanism in the coagulation process using Al or Al as coagulants was electrostatic patching and the main mechanism using AS was charge neutralization. In the and systems, when Al and Al were used as coagulants at low dosage, the coagulants that were adsorbed on the side (which existed on the surfaces of and cells) would lose their ability to aggregate the algal cells. When AS was used as coagulant, the electric double layer was effectively compressed. The repulsive force between algal particles decreased, and the flocs formed easily. In the system, Al and Alcoagulants effectively formed the flocs through electrostatic patch effects. There was a significant correlation between membrane flux and floc size, and the larger flocs formed a looser cake layer on the membrane surface.
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http://dx.doi.org/10.13227/j.hjkx.201701130DOI Listing
August 2017

Topological Qubits from Valence Bond Solids.

Phys Rev Lett 2018 May;120(20):200503

Department of Physics and Astronomy, and Stewart Blusson Quantum Matter Institute, University of British Columbia, Vancouver, Canada.

Topological qubits based on SU(N)-symmetric valence-bond solid models are constructed. A logical topological qubit is the ground subspace with twofold degeneracy, which is due to the spontaneous breaking of a global parity symmetry. A logical Z rotation by an angle 2π/N, for any integer N>2, is provided by a global twist operation, which is of a topological nature and protected by the energy gap. A general concatenation scheme with standard quantum error-correction codes is also proposed, which can lead to better codes. Generic error-correction properties of symmetry-protected topological order are also demonstrated.
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http://dx.doi.org/10.1103/PhysRevLett.120.200503DOI Listing
May 2018

Efficacy and Safety of a Formulated Herbal Granula, Jiu Wei Zhen Xin, for Generalized Anxiety Disorder: A Meta-Analysis.

Evid Based Complement Alternat Med 2018 7;2018:9090181. Epub 2018 Mar 7.

Institute of Diagnosis of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410218, China.

Background: The traditional Chinese medicine formula Jiu Wei Zhen Xin Granula (JWZXG) is prescribed to treat generalized anxiety disorder (GAD) in China. This study was to assess the efficacy and safety of JWZXG in patients with GAD.

Method: Data were pooled from 14 randomized controlled trials involving the assessment of mean changes of Hamilton Anxiety Rating Scale (HAMA) total scores, response rates, adverse event rates, quality, publication bias, and risk of bias.

Results: Pooled analysis showed no significant difference in response rate (risk ratio 1.01, 95% CI [0.93-1.08]; test = 0.17, = 0.86) and no significant difference between JWZXG group and azapirones group (RR 0.69, 95% CI [0.45, 1.06]; test = 1.69, = 0.09) in rate of adverse events. Though no difference exists between JWZXG group and azapirones group in HAMA total score from baseline, JWZXG group was inferior to selective serotonin reuptake inhibitors (SSRIs) group (WMD -0.93, 95% CI [-1.64, -0.23]; test = 2.6, = 0.009) which had more adverse events than JWZXG group (RR 0.64, 95% CI [0.46, 0.89]; test = 2.63, = 0.009).

Conclusions: This meta-analysis preliminarily suggests that JWZXG is as effective as azapirones, though having the same possibility of suffering AEs. JWZXG was inferior to SSRIs but causes fewer AEs in the treatment of GAD.
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http://dx.doi.org/10.1155/2018/9090181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863357PMC
March 2018

[Effects of lime-ammonium bicarbonate fumigation and biofertilizer application on Fusarium wilt and biomass of continuous cropping cucumber and watermelon.]

Ying Yong Sheng Tai Xue Bao 2017 Oct;28(10):3351-3359

College of Resources and Environmental Sciences/Jiangsu Province Key Laboratory for Organic Solid Waste Utilization/Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China.

In this study, the population size of soil microbes was determined using plate counting method after the application of lime-ammonium bicarbonate and ammonium bicarbonate fumigation. In addition, biofertilizer was applied after soil fumigation and population of Fusarium oxysporum, Fusarium wilt disease control efficiency and plant biomass were determined in the cucumber and watermelon continuous cropping soil. The results showed that the population of F. oxysporum in cucumber mono-cropped soil fumigated with lime-ammonium bicarbonate or ammonium bicarbonate was decreased by 95.4% and 71.4%, while that in watermelon mono-cropped soil was decreased by 87.3% and 61.2%, respectively compared with non-fumigated control (CK). Furthermore, the greenhouse experiment showed that biofertilizer application, soil fumigation and crop type showed significant effects on the number of soil F. oxysporum, Fusarium wilt disease incidence, disease control efficiency and plant biomass based on multivariate analysis of variance. In the lime-ammonium bicarbonate fumigated soil amended with biofertilizer (LFB), significant reductions in the numbers of F. oxysporum and Fusarium wilt disease incidence were observed in both cucumber and watermelon cropped soil compared to non-fumigated control soil applied with organic fertilizer. The disease control rate was 91.9% and 92.5% for cucumber and watermelon, respectively. Moreover, LFB also significantly increased the plant height, stem diameter, leaf SPAD, and dry biomass for cucumber and watermelon. It was indicated that biofertilizer application after lime-ammonium bicarbonate fumigation could effectively reduce the abundance of F. oxysporum in soil, control Fusarium wilt disease and improve plant biomass in cucumber and watermelon mono-cropping systems.
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http://dx.doi.org/10.13287/j.1001-9332.201710.036DOI Listing
October 2017

Evaluation of Two Commercial Enzyme-Linked Immunosorbent Assay Kits for the Detection of Human Circulating Metrnl.

Chem Pharm Bull (Tokyo) 2018 Apr 2;66(4):391-398. Epub 2018 Feb 2.

Department of Pharmacology, Second Military Medical University.

Metrnl is a newly discovered secreted protein with neurotrophic activity and metabolic effect, while in earlier studies its circulating level in human was not explored. We evaluated two commercial enzyme-linked immunosorbent assay kits (DY7867-05, R&D Systems and SK00478-02, Aviscera Bioscience) for the detection of human circulating Metrnl. The DY7867-05 kit showed superiority over the SK00478-02 kit since it generated better curve fitting degree, smaller variation among tests, higher inter-assay reproducibility and better specificity, and could effectively detect human Metrnl in six types of blood samples. Subsequent analysis was performed using the DY7867-05 kit. Sample storage conditions were investigated. No gender difference in circulating Metrnl levels was found, while people with newly diagnosed type 2 diabetes mellitus (T2DM) had significantly lower Metrnl levels compared to the healthy controls.
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http://dx.doi.org/10.1248/cpb.c17-00846DOI Listing
April 2018

Differential proteomic profiling of endometrium and plasma indicate the importance of hydrolysis in bovine endometritis.

J Dairy Sci 2017 Nov 13;100(11):9324-9337. Epub 2017 Sep 13.

Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Lanzhou 730050, China. Electronic address:

Endometritis is an important disease of dairy cows that leads to significant economic losses in the dairy cattle industry. To investigate the alteration of proteins associated with endometritis in the dairy cow, the isobaric tags for relative and absolute quantification (iTRAQ) technique was applied to quantitatively identify differentially expressed proteins (DEP) in the endometrium and peripheral plasma of Chinese Holstein cows with endometritis. Compared with the normal (control) group, 159 DEP in the endometrium and 137 DEP in the plasma were identified in cows with endometritis. Gene ontology analysis demonstrated that the predominant endometrial DEP were primarily involved in responses to stimulus and stress processes and mainly played a role in hydrolysis in the extracellular region. The predominant plasma DEP were mainly components of the cytosol and non-membrane-bound organelles, and they were involved in the response to stress and regulation of enzyme activity. Protein-protein interaction of tissue DEP revealed that some core seed proteins, such as RAC2, ITGB2, and CDH1 in the same network as CD14, MMP3, and MMP9, had important functions in the cross-talk of pathways related to extracellular proteolysis. In summary, significant enzymatic hydrolase activity in the extracellular region is proposed as a molecular mechanism by which altered proteins may promote inflammation and hence endometritis.
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http://dx.doi.org/10.3168/jds.2016-12365DOI Listing
November 2017

Computational Power of Symmetry-Protected Topological Phases.

Phys Rev Lett 2017 Jul 5;119(1):010504. Epub 2017 Jul 5.

Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada.

We consider ground states of quantum spin chains with symmetry-protected topological (SPT) order as resources for measurement-based quantum computation (MBQC). We show that, for a wide range of SPT phases, the computational power of ground states is uniform throughout each phase. This computational power, defined as the Lie group of executable gates in MBQC, is determined by the same algebraic information that labels the SPT phase itself. We prove that these Lie groups always contain a full set of single-qubit gates, thereby affirming the long-standing conjecture that general SPT phases can serve as computationally useful phases of matter.
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http://dx.doi.org/10.1103/PhysRevLett.119.010504DOI Listing
July 2017

[Influencing Mechanism of Calcium Peroxide Pre-treatment on Dewatering Performance of Waste Activated Sludge].

Huan Jing Ke Xue 2017 Mar;38(3):1151-1158

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

The evolution of sludge filteration dewaterability, floc structure and hydrolytic kinetics with calcium peroxide oxidation pre-treatment was investigated in this study. The effect of sludge characteristics with combined process of ferrous ion and calcium peroxide was deeply analyzed and the result suggested that the sludge dewatering performance was improved first and then worsened after CaO addition. When the dosage was 20 mg·g, sludge reached its optimal dewaterability. At the same time, sludge was effectively dissolved and the floc structure became loose and broken with the increase of organic matters in the supernatant. Moreover, sludge solubilization process followed the pseudo-zero-order kinetic equation well and the reaction rate of sludge with CaO treatment was 15.2 mg·L·h. In addition, sludge floc lysis was enhanced by the treatment of ferrous ion and calcium peroxide oxidation, whilst sludge dewaterability was improved due to the reconstruction of sludge floc structure by the iron ions produced. This study provided theoretical basis for application of calcium peroxide pre-treatment and its combining technique in sludge treatment.
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http://dx.doi.org/10.13227/j.hjkx.201607056DOI Listing
March 2017

Three-dimensional investigation of ozone pollution in the lower troposphere using an unmanned aerial vehicle platform.

Environ Pollut 2017 May 12;224:107-116. Epub 2017 Feb 12.

Center for Intelligent Transportation Systems and Unmanned Aerial Systems Applications Research, State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Potential utilities of instrumented lightweight unmanned aerial vehicles (UAVs) to quickly characterize tropospheric ozone pollution and meteorological factors including air temperature and relative humidity at three-dimensional scales are highlighted in this study. Both vertical and horizontal variations of ozone within the 1000 m lower troposphere at a local area of 4 × 4 km are investigated during summer and autumn times. Results from field measurements show that the UAV platform has a sufficient reliability and precision in capturing spatiotemporal variations of ozone and meteorological factors. The results also reveal that ozone vertical variation is mainly linked to the vertical distribution patterns of air temperature and the horizontal transport of air masses from other regions. In addition, significant horizontal variations of ozone are also observed at different levels. Without major exhaust sources, ozone horizontal variation has a strong correlation with the vertical convection intensity of air masses within the lower troposphere. Higher air temperatures are usually related to lower ozone horizontal variations at the localized area, whereas underlying surface diversity has a week influence. Three-dimensional ozone maps are obtained using an interpolation method based on UAV collected samples, which are capable of clearly demonstrating the diurnal evolution processes of ozone within the 1000 m lower troposphere.
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http://dx.doi.org/10.1016/j.envpol.2017.01.064DOI Listing
May 2017

Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3'-Hydroxylation Using Recombinant Escherichia coli.

Molecules 2016 Dec 15;21(12). Epub 2016 Dec 15.

Department of Biological Sciences and Technology, National University of Tainan, Tainan 70005, Taiwan.

The present study describes the biotransformation of a commercially available crude extract of soy isoflavones, which contained significant amounts of the soy isoflavone glycosides daidzin and genistin, by recombinant expressing tyrosinase from . Two major products were isolated from the biotransformation and identified as 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively, based on their mass and nuclear magnetic resonance spectral data. The two 3'-hydroxyisoflavone glycosides showed potent 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity with IC values of 7.4 and 9.8 μM for 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively. The free radical scavenging activities of the two 3'-hydroxyisoflavone glycosides were, respectively, 120 and 72 times higher than the activity of their precursors, daidzin and genistin, and were also stronger than the activity of ascorbic acid, which showed an IC value of 15.1 μM. This is the first report of the bio-production and potential antioxidant applications of both 3'-hydroxydaidzin and 3'-hydroxygenistin.
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http://dx.doi.org/10.3390/molecules21121723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273682PMC
December 2016

Analgesic effects of adenylyl cyclase inhibitor NB001 on bone cancer pain in a mouse model.

Mol Pain 2016 9;12. Epub 2016 Sep 9.

Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, China Center for Neuron and Disease, Frontier Institutes of Life Science and of Science and Technology, Xi'an Jiaotong University, Xi'an, China

Background: Cancer pain, especially the one caused by metastasis in bones, is a severe type of pain. Pain becomes chronic unless its causes and consequences are resolved. With improvements in cancer detection and survival among patients, pain has been considered as a great challenge because traditional therapies are partially effective in terms of providing relief. Cancer pain mechanisms are more poorly understood than neuropathic and inflammatory pain states. Chronic inflammatory pain and neuropathic pain are influenced by NB001, an adenylyl cyclase 1 (AC1)-specific inhibitor with analgesic effects. In this study, the analgesic effects of NB001 on cancer pain were evaluated.

Results: Pain was induced by injecting osteolytic murine sarcoma cell NCTC 2472 into the intramedullary cavity of the femur of mice. The mice injected with sarcoma cells for four weeks exhibited significant spontaneous pain behavior and mechanical allodynia. The continuous systemic application of NB001 (30 mg/kg, intraperitoneally, twice daily for three days) markedly decreased the number of spontaneous lifting but increased the mechanical paw withdrawal threshold. NB001 decreased the concentrations of cAMP and the levels of GluN2A, GluN2B, p-GluA1 (831), and p-GluA1 (845) in the anterior cingulate cortex, and inhibited the frequency of presynaptic neurotransmitter release in the anterior cingulate cortex of the mouse models.

Conclusions: NB001 may serve as a novel analgesic to treat bone cancer pain. Its analgesic effect is at least partially due to the inhibition of AC1 in anterior cingulate cortex.
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http://dx.doi.org/10.1177/1744806916652409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019365PMC
October 2017

Sinomenine attenuates chronic inflammatory pain in mice.

Metab Brain Dis 2017 02 1;32(1):211-219. Epub 2016 Sep 1.

Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.

Sinomenine, an alkaloid originally isolated from the roots of Sinomeniumacutum, is used as a traditional Chinese medicine for rheumatic arthritis. However, little is known about the neuronal mechanisms underlying the analgesic effects of sinomenine in animals with chronic inflammatory pain. In this study, we investigated the persistent inflammatory pain induced by hind paw injection of complete Freund's adjuvant (CFA) in mice, which was reversed by sinomenine administration. In the anterior cingulate cortex (ACC), a region highly associated with chronic pain processing, the upregulation of GluN2B-containing N-methyl-D-aspartate (NMDA) receptors and Ca/calmodulin-dependent protein kinase II, total levels of GluA1, and phosphorylation of GluA1 at Ser831 (p-GluA1-Ser831) were reversed by systemically administrating sinomenine. Furthermore, sinomenine treatment downregulated the mammalian target of rapamycin (mTOR) pathway. Increases in p-mTOR, p-p70S6k, p-S6, and p-4EBP, which were induced by chronic inflammation, were all changed. However, sinomenine did not affect the levels of GluN2A-containing NMDA receptors and p-GluA1-Ser845, as well as the total levels of mTOR, p70S6k, S6, and 4EBP. In conclusion, results indicated that sinomenine reduced the chronic inflammatory pain induced by CFA, at least partially by regulating the GluN2B receptors and mTOR signals in the ACC.
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http://dx.doi.org/10.1007/s11011-016-9889-8DOI Listing
February 2017