Publications by authors named "Dong-mei Liu"

91 Publications

DMDL 9010 alleviates dextran sodium sulfate (DSS)-induced colitis and behavioral disorders by facilitating microbiota-gut-brain axis balance.

Food Funct 2022 Jan 4;13(1):411-424. Epub 2022 Jan 4.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China.

Previous studies have found that probiotic supplements can ameliorate mental behavioral disorders. This study investigated the effects of DMDL 9010 (LP9010) intake on the depression-like behavior induced by dextran sodium sulfate (DSS) and its possible mechanism. Male C57BL/6N mice were fed with DSS to establish the model of ulcerative colitis. LP9010 intake reduced the DSS-induced inflammatory response, and repaired intestinal barrier damage, as well as lightened depression-like behavior. LP9010 supplementation also inhibited neuroinflammation by up-regulating the levels of neurotransmitters, especially 5-HT, NE, DA, and 5-HIAA. Moreover, the intake of LP9010 reorganized the gut microbiome by increasing the relative abundance of Bacteroidetes and Firmicutes, and decreasing the relative abundance of Proteobacteria and Verrucomicrobia. Furthermore, treatment with LP9010 increased the levels of short-chain fatty acids, such as butyric acid and propionic acid. In conclusion, LP9010 intake was a promising probiotic intervention strategy for the prevention of colitis-induced behavioral disorders through the microbiota-gut-brain axis.
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http://dx.doi.org/10.1039/d1fo02938jDOI Listing
January 2022

Two New Species of (, ) with a Key to Worldwide Species.

J Fungi (Basel) 2021 Nov 18;7(11). Epub 2021 Nov 18.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

is a genus of wood-inhabiting fungi consisting of four species so far, including as generic type. Two new species, and , are described and illustrated from China. from southwestern China is characterized by a grandinioid to odontioid hymenophore with numerous small aculei, a dimitic hyphal system with scattered, smooth skeletal hyphae and ellipsoid basidiospores measuring 4.2-5.2 × 3.5-4.5 μm. from the West Tianshan Mountain in northwestern China is distinguished by an odontioid to hydnoid hymenophore, a dimitic hyphal system, and ellipsoid basidiospores measuring 3.7-4.4 × 2.8-3.4 μm. The phylogenies inferred from the data set of nuc rDNA ITS1-5.8S-ITS2 (ITS) and D1-D2 domains of nuc 28S rDNA (28S), and that of ITS, 28S, translation elongation factor (), and RNA polymerase II second largest subunit () supported as a monophyletic genus in the and and as separate lineages within . Multi-rate Poisson Tree Processes, Automatic Barcode Gap Discovery and genetic distance methods based on ITS sequences of also supported and as distinct species. The taxonomic status of that was recently transferred from , is briefly discussed. A key to all six known species of is provided.
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http://dx.doi.org/10.3390/jof7110982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626033PMC
November 2021

First Report of Powdery Mildew Caused By on Scarlet Beebalm () in China.

Plant Dis 2021 Nov 11. Epub 2021 Nov 11.

Shangqiu Normal University, 117757, Life Science, 289, Wenhua Rd., Shangqiu, Henan, China, 476000;

Scarlet Beebalm (Monarda didyma) is a perennial ornamental flowering plant in the mint family, Labiatae. Due to low-maintenance, and a long blooming period, it is commonly cultivated in gardens as an ornamental plant in China. In May 2021, a disease was observed on the leaves of a capitals beebalm plant in a Ten Mile Flower Sea in Xiayi county (116°13'8″E, 34°14'45″N), Henan province of China. Symptoms first appeared as nearly circular, small, white, powdery mildew-like spots on the leaves which gradually expand, covering the entire leaves. The lesions spread from the lower leaves to the upper canopy, and the stems were also covered by white mycelia. In severe cases, early defoliation occured. About 30% plants were affected. Representative voucher specimens (SQNUMd04, SQNUDn4) were deposited in the herbarium of Shangqiu Normal University (SQNU), Shangqiu, China. Conidiophores (n = 30) were cylindrical, 92.0 to 142.2 µm long and 10.8 to 14.2 µm wide, and produced 5 to 7 immature conidia in a chain. Foot-cells of conidiophores were mostly curved at the base. Conidia (n = 30) were hyaline, ellipsoid, 23.3 to 29.8 μm (avg. 26.6 μm) long, and 11.2 to 16.9 μm (avg. 14.5μm) width, a length/width ratio of 1.5 to 2.1, and germ tubes were produced at the perihilar position. No chasmothecia were observed. Based on morphological comparison using the description by Scholler et al. (2016) description the fungus was tentatively identified as Golovinomyces monardae (G.S. Nagy) M. Scholler, U. Braun & Anke Schmidt. For molecular identification, DNA was extracted from mycelia and conidia, which were collected by scraping symptomatic leaves.The ITS regions and LSU were amplified using primers ITS1/ITS4 (White et al. 1990) and NL1/NL4 (Horisawa et al. 2013). BLASTn analysis of the (MZ303741) and LSU (MZ305434) sequences showed 100% identity with those of G. monardae (AB307667, LC076800, LC076802, LC076808, and AB077691) reported on Monarda species (Matsuda et al. 2003; Takamatsu et al. 2013; Scholler et al. 2016). Pathogenicity tests were carried out by gently dusting conidia from infected leaves onto healthy leaves of five M. didyma plants and five non-inoculated plants used as controls. After 9 days, typical powdery mildew colonies started to appear on the inoculated leaves while control plants remained disease free. All plants were placed in a greenhouse without temperature and humidity control. Based on morphology, fungus was identified as the same as that used for inoculum, fulfilling Koch's postulates. Although G. monardae has been reported on various genera in the Labiatae and Verbenaceae (Farr and Rossman 2021), to our knowledge, this is the first report of G. monardae causing powdery mildew of Scarlet Beebalm(M. didyma) in China.
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http://dx.doi.org/10.1094/PDIS-08-21-1803-PDNDOI Listing
November 2021

Drugs affecting renin-angiotensin-aldosterone system and the cancer risk: A meta-analysis of nested case-control studies.

Pak J Pharm Sci 2021 Jan;34(1):35-39

Cardiology Department of Yulin First Hospital, Second Affiliated Hospital of Yanan University, Yulin Shaanxi, PR China.

Multiple studies have discussed the associations between drugs affecting the renin-angiotensin-aldosterone system and the cancer risk, but their consequence s were conflicting. A meta-analysis of nested case-control studies published regarding this subject was conducted in our study, aims to estimate the association between ACEI/ARB and the cancer risk. Pubmed database was searched up to February, 1 2016 to identify eligible nested case-control studies, and we used Newcastle-Ottawa Scale (NOS) to assess quality of the studies. Pooled odds ratio (OR) and 95% confidence intervals (CIs) were calculated (with fixed effect model: Mantel-Haenszel). Publication bias and heterogeneity were evaluated before the calculation. Subgroup analysis and sensitivity analysis were also performed. Seven studies contributed to the analysis. Overall, ACEI/ARB use was not associated with the risk of cancer (OR=0.99, 95% CI 0.97-1.01), nor in long-term use patients (OR=0.97, 95% CI 0.92-1.01). ACEI may decrease cancer risk (OR=0.90, 95% CI 0.82-0.99). We observed no significant publication bias. In conclusion, ACEI/ARB use was not associated with cancer risk, nor in long-term use patients, but ACEI use may decrease cancer risk. More researches are needed to confirm these findings.
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January 2021

PPARγ2 functions as a tumor suppressor in a translational mouse model of human prostate cancer.

Asian J Androl 2022 Jan-Feb;24(1):90-96

Laboratory of Nuclear Receptors and Cancer Research, Department of Pathology, Medical School, Nantong University, Nantong 226001, China.

Peroxisome proliferator-activated receptors γ (PPARγ) is a master regulator that controls energy metabolism and cell fate. PPARγ2, a PPARγ isoform, is highly expressed in the normal prostate but expressed at lower levels in prostate cancer tissues. In the present study, PC3 and LNCaP cells were used to examine the benefits of restoring PPARγ2 activity. PPARγ2 was overexpressed in PC3 and LNCaP cells, and cell proliferation and migration were detected. Hematoxylin and eosin (H&E) staining was used to detect pathological changes. The genes regulated by PPARγ2 overexpression were detected by microarray analysis. The restoration of PPARγ2 in PC3 and LNCaP cells inhibited cell proliferation and migration. PC3-PPARγ2 tissue recombinants showed necrosis in cancerous regions and leukocyte infiltration in the surrounding stroma by H&E staining. We found higher mixed lineage kinase domain-like (MLKL) and lower microtubule-associated protein 1 light chain 3 (LC3) expression in cancer tissues compared to controls by immunohistochemistry (IHC) staining. Microarray analysis showed that PPARγ2 gain of function in PC3 cells resulted in the reprogramming of lipid- and energy metabolism-associated signaling pathways. These data indicate that PPARγ2 exerts a crucial tumor-suppressive effect by triggering necrosis and an inflammatory reaction in human prostate cancer.
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http://dx.doi.org/10.4103/aja.aja_51_21DOI Listing
January 2022

An ultrasound observation study on the levator hiatus with or without diastasis recti abdominis in postpartum women.

Int Urogynecol J 2021 07 17;32(7):1839-1846. Epub 2021 Apr 17.

Department of Ultrasonography, The Second Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Nanggang District, Harbin, 150081, People's Republic of China.

Introduction And Hypothesis: We hypothesized that differences in post-partum levator hiatus (LH) measurements, as well as the area of urethra and bladder (AUB), viewed under ultrasound, correlate with diastasis rectus abdominis (DRA) occurrence. The primary objective of this study is to determine ultrasound parameters available for diagnosing DRA in post-partum women. We compared LH and AUB measurements under ultrasound in primiparous women, with and without DRA, at 24-26 weeks postpartum.

Methods: One hundred ninety-four women underwent routine examination, including a self-made clinical symptoms questionnaire, DRA evaluation, and LH and AUB measurements. Independent samples t- and chi-squared tests were used to compare the differences between women with and without DRA.

Results: DRA incidence was significantly higher among those who underwent cesarean section (CS) than for vaginal delivery (VD) (P = 0.038). DRA patients could potentially have urinary urgency, frequency, pain, dysuria, and perineal tears. Additionally, statistically significant differences were found between VD patients, with or without DRA, in the resting LH transverse diameter (TrD) (P = 0.032) and the area of the levator hiatus (ALH) (P = 0.048) as well as AUB at Valsalva (P = 0.049). No differences, however, were found between the DRA and no DRA groups for all those measurements among women who had cesarean deliveries.

Conclusions: DRA was more likely in post-CS women. Furthermore, the results showed a plausible association between DRA occurrence and LH expansion, especially in women with VD under rest and Valsalva. This could be useful for developing therapeutic plans based on these parameters for post-partum rehabilitation of women with DRA to avoid long-term complications.
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http://dx.doi.org/10.1007/s00192-021-04783-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295084PMC
July 2021

Bacillus coagulans 13002 and fructo-oligosaccharides improve the immunity of mice with immunosuppression induced by cyclophosphamide through modulating intestinal-derived and fecal microbiota.

Food Res Int 2021 02 16;140:109793. Epub 2020 Oct 16.

School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, Guangdong 510641, People's Republic of China. Electronic address:

This study aims to evaluate the effects of probiotic Bacillus coagulans 13,002 (BCS) and prebiotic fructo-oligosaccharides (FOS) on mice treated with the alkylating agent cyclophosphamide (CTX). We found that both BCS and FOS, especially BCS, significantly alleviated CTX-induced injury by modulating intestinal-derived and fecal microbiota. BCS and BCS + FOS increased serum immunoglobulin levels, which were reduced by CTX. In addition, BCS and BCS + FOS upregulated IFN-γ and IL-4, which protect mucosal barriers and the balance of Th1/Th2. BCS promoted the growth of some beneficial bacteria, such as Bacteroides, Coprococcus, Enterococcus, Oscillospira, and Ruminococcus in mouse gut. In addition, BCS + FOS inhibited the growth of several harmful bacteria, including Acinetobacter, Arthrobacter, Brachybacterium, Corynebacterium, Jeotgalicoccus, Sporosarcina, and Staphylococcus. Furthermore, BCS potentially improved the growth of Anaerotruncus bacteria, which can promote the production of butyrate acids. In summary, according our results suggest that BCS and FOS improved the immunity of mice with immunosuppression induced by CTX through modulating intestinal-derived and fecal microbiota.
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http://dx.doi.org/10.1016/j.foodres.2020.109793DOI Listing
February 2021

Regulation of carotenoid degradation and production of apocarotenoids in natural and engineered organisms.

Crit Rev Biotechnol 2021 Jun 4;41(4):513-534. Epub 2021 Feb 4.

School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Carotenoids are important precursors of a wide range of apocarotenoids with their functions including: hormones, pigments, retinoids, volatiles, and signals, which can be used in the food, flavors, fragrances, cosmetics, and pharmaceutical industries. This article focuses on the formation of these multifaceted apocarotenoids and their diverse biological roles in all living systems. Carotenoid degradation pathways include: enzymatic oxidation by specific carotenoid cleavage oxygenases (CCOs) or nonspecific enzymes such as lipoxygenases and peroxidases and non-enzymatic oxidation by reactive oxygen species. Recent advances in the regulation of carotenoid cleavage genes and the biotechnological production of multiple apocarotenoids are also covered. It is suggested that different developmental stages and environmental stresses can influence both the expression of carotenoid cleavage genes and the formation of apocarotenoids at multiple levels of regulation including: transcriptional, transcription factors, posttranscriptional, posttranslational, and epigenetic modification. Regarding the biotechnological production of apocarotenoids especially: crocins, retinoids, and ionones, enzymatic biocatalysis and metabolically engineered microorganisms have been a promising alternative route. New substrates, carotenoid cleavage enzymes, biosynthetic pathways for apocarotenoids, and new biological functions of apocarotenoids will be discussed with the improvement of our understanding of apocarotenoid biology, biochemistry, function, and formation from different organisms.
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http://dx.doi.org/10.1080/07388551.2021.1873242DOI Listing
June 2021

DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea.

Microorganisms 2020 Oct 24;8(11). Epub 2020 Oct 24.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

Antibiotic-associated diarrhea (AAD) is the most common side effect of antibiotics and is routinely treated with probiotics in clinical. , extensively utilized for producing dairy foods, has recently been regarded as a new promising probiotic candidate. In this study, the efficacy of DMST-H2 (DMST-H2) for AAD treatment in mice was investigated. DMST-H2 was isolated from Chinese traditional yogurt, proved to be non-toxic, and presented tolerance against simulated gastrointestinal conditions . Additionally, genomic analysis revealed that it possessed genes related to acid tolerance, bile salt tolerance, adhesion, oxidative stress and bacteriocin production. The animal experiment results showed that both DMST-H2 treatment and natural recovery could reduce fecal water content. Compared with spontaneous recovery, DMST-H2 accelerated the recovery of the enlarged caecum and intestinal barrier injury from AAD, and further decreased endotoxin (ET), D-lactate (D-LA) and diamine oxidase (DAO) content in serum. Moreover, pro-inflammatory cytokines (TNF-α) were reduced, while interferon-γ (IFN-γ) and anti-inflammatory cytokines (IL-10) increased after treating with DMST-H2. Furthermore, DMST-H2 better restored the structure of intestinal flora. At the phylum level, Firmicutes increased and Proteobacteria decreased. These findings indicate that DMST-H2 could promote recovery in mice with antibiotic-associated diarrhea.
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http://dx.doi.org/10.3390/microorganisms8111650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693992PMC
October 2020

A colorimetric method for screening α-glucosidase inhibitors from flavonoids using 3,3',5,5'-tetramethylbenzidine as a chromogenic probe.

Colloids Surf B Biointerfaces 2021 Jan 19;197:111400. Epub 2020 Oct 19.

College of Science, Gansu Agricultural University, Lanzhou, 730000, Gansu Province, PR China. Electronic address:

A facile and novel colorimetric method for screening of α-glucosidase inhibitors (AGIs) from flavonoids using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic probe is proposed. This method is based on the colorimetric detection of ascorbic acid (AA) through the TMB oxidation reaction catalyzed by horseradish peroxidase (HRP) in the presence of hydrogen peroxide (HO). In the TMB/HO/HRP system, HRP catalyzes the oxidation of HO to ‧OH radical which oxidizes TMB to blue-colored oxidized TMB (oxTMB). In the presence of AA, the production of ‧OH radical is suppressed and causes the decrease of oxTMB, resulting in the fading of the blue color and the decrease of absorbance at 652 nm. Based on this, the existence of AA can be facilely identified. In the 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2 G)/α-glucosidase (α-Glu) system, the produced AA inhibits the oxidation of TMB to blue-colored oxTMB. In the presence of AGIs, the production of AA is inhibited, which inhibits the reduction of oxTMB, resulting in a blue color recovery and an increase of the absorbance at 652 nm. Based on this, the colorimetric method is developed for screening of AGIs from 7 flavonoids.
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http://dx.doi.org/10.1016/j.colsurfb.2020.111400DOI Listing
January 2021

Aberrant Expression of Cancer-Testis Antigen FBXO39 in Breast Cancer and its Clinical Significance.

Clin Lab 2020 Sep;66(9)

Background: Cancer/testis antigen (CTA) is a class of antigen molecules expressed only in the germinal epithelium of testis and some tumor tissues. As an important CTA molecule, the expression of F-box protein 39 (FBXO39) in breast cancer (BC) and its clinical significance remain unclear. The objective of this study is to explore the value of FBXO39 in the diagnosis, efficacy monitoring, and prognostic evaluation of BC.

Methods: The expression of FBXO39 mRNA in the serum exosomes of patients with BC before and after the initial diagnosis and treatment was detected by qRT-PCR, and the corresponding ROC curve was plotted. The expression of FBXO39 protein in BC cancer tissues was detected by immunohistochemistry, along with the analysis of the correlation between FBXO39 expression and clinical pathological features as well as prognosis of BC cases.

Results: The serum-derived exosomes were successfully isolated and identified. The positive rate of FBXO39 mRNA in serum exosomes of patients with BC was up to 86%; there was a correlation between the expression level of serum exosomal FBXO39 and clinical staging, HER2, and Ki-67 expression (all with p < 0.05). The sensitivity of serum exosomal FBXO39 in distinguishing BC patients from healthy controls was 88%, with the specificity as 86%, and AUC as 0.9432. The expression change of FBXO39 in serum-sourced exosomes of patients with BC was related to their treatment situation, indicating that the level of FBXO39 decreased significantly after treatment. The expression of FBXO39 in cancer tissue was related to the clinical stage (p = 0.023) and lymphatic metastasis (p = 0.015) of the BC patients. Survival analysis showed that the expression of FBXO39 was negatively correlated with the prognosis of BC patients, with the high expression of FBXO39 indicating poor prognosis.

Conclusions: Serum-derived exosomal FBXO39 could serve as an important indicator of BC diagnosis and efficacy evaluation; FBXO39 could be rated as an important indicator of BC prognosis evaluation.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200121DOI Listing
September 2020

Facile preparation of chitosan modified magnetic kaolin by one-pot coprecipitation method for efficient removal of methyl orange.

Carbohydr Polym 2020 Oct 11;245:116572. Epub 2020 Jun 11.

School of Resources and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou, 341000, PR China. Electronic address:

Chitosan modified magnetic kaolin (CS/kaolin/FeO) composite was prepared by a facile one-pot coprecipitation method and used for the removal of methyl orange (MO) from aqueous medium. Under alkaline condition, FeO nanoparticles were deposited on the kaolin layer by in-situ growth method and chitosan was deposited on the kaolin layer by pH-precipitation method. With the modification of CS, adsorption sites for anionic species were introduced onto the adsorbent. The prepared CS/kaolin/FeO could remove more than 94 % of MO and showed a high saturated adsorption capacity of 349.7 mg/g. The adsorption process was controlled by film diffusion and well described by Langmuir model. The thermodynamic studies indicated that the adsorption process was exothermic in nature. Furthermore, the adsorbent exhibited satisfactory recycle ability. The results suggested that the modification with CS broadened the application scope of kaolin in anionic species removal and the CS/kaolin/FeO composite could be a promising adsorbent for wastewater treatment.
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http://dx.doi.org/10.1016/j.carbpol.2020.116572DOI Listing
October 2020

Potential risk of hyperuricemia: leading cardiomyocyte hypertrophy by inducing autophagy.

Am J Transl Res 2020 15;12(5):1894-1903. Epub 2020 May 15.

Department of Rheumatology, Zhongshan Hospital, Fudan University Shanghai 200030, China.

Background: Clinical studies have shown that hyperuricemia is associated with many cardiovascular diseases; however, the mechanisms involved remain unclear. In this study, we investigated the effect of uric acid on cardiomyocytes and the underlying mechanism.

Methods And Results: H9c2 cardiomyocytes were treated with various concentrations of uric acid. 3-Methyladenine (3-MA) or Compound C was added before treatment with uric acid. The expression of myocardial hypertrophy-related genes was measured using polymerase chain reaction (PCR). The cell surface area was calculated using ImageJ Software. Western blotting was used to measure the protein levels. Uric acid increased the gene expression of , , and , which are involved in myocardial hypertrophy, and the relative cell surface area of cardiomyocytes in a dose-dependent manner. Consistently, the ratio of LC3II/I, which is a biomarker of autophagy, increased dose-dependently, whereas the protein level of p62, a protein that is degraded by autophagy, decreased. 3-MA, an autophagy inhibitor, rescued uric acid-induced myocardial hypertrophy. Treatment with uric acid increased the level of phosphorylated adenosine monophosphate kinase (AMPK), as well as its downstream effector unc-51-like kinase (ULK1). Pharmacological inhibition of AMPK by Compound C attenuated the uric acid-induced activation of autophagy and myocardial hypertrophy.

Conclusions: Uric acid induces myocardial hypertrophy by activating autophagy via the AMPK-ULK1 signaling pathway. Decreasing the serum uric acid level may therefore be clinically beneficial in alleviating cardiac hypertrophy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269983PMC
May 2020

ATP6V0D2, a subunit associated with proton transport, serves an oncogenic role in esophagus cancer and is correlated with epithelial-mesenchymal transition.

Esophagus 2020 10 2;17(4):456-467. Epub 2020 Apr 2.

Department of Pharmacy, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, The East courtyard, No. 42 of West Culture Road, Lixia district, Jinan, 250014, Shandong, People's Republic of China.

Background: The poor prognosis of esophagus cancer (EC) is mainly due to its high invasiveness and metastasis, so it is urgent to search effectively prognostic markers and explore their roles in the mechanism of metastasis.

Materials And Methods: Based on the TCGA database, we downloaded the RNA-Seq for analyzing the expression of ATP6V0D2. QRT-PCR was used to test the mRNA levels of ATP6V0D2 in cell lines. Chi-square tests were used to evaluate the correlation between ATP6V0D2 and clinical characteristics. Prognostic values were determined by Kaplan-Meier methods and cox's regression models. CCK-8 and clone formation assays were employed to evaluate the cell viability, and Transwell assay was implemented to determine the invasive and migratory abilities. Correlations between ATP6V0D2 and motion-related markers were analyzed by the GEPIA database and confirmed by western blot. Moreover, the relationship between ATP6V0D2 and molecules related to cell cycle and apoptosis was also determined by western blot.

Results: A significant increase was observed in 3 EC-related cell lines compared to the normal cell line. ATP6V0D2 has a connection with the poor prognosis and can be considered as an independent prognosticator for patients with EC. Besides, ATP6V0D2 can improve cells viability as well as invasive and migratory abilities. What's more, downregulation of ATP6V0D2 notably enhanced E-cadherin expression, while decreased N-cadherin, Vimentin, and MMP9 expression, whereas overexpression of ATP6V0D2 presented the opposite outcomes. Furthermore, we found that silencing ATP6V0D2 led to a significant reduction on the protein expression of Cyclin D1, CDK4, Bcl-2, whereas resulted in a notable enhancement on the Bax level.

Conclusion: ATP6V0D2 might be an independent prognosticator for EC patients, and it possibly promotes tumorigenesis by regulating epithelial-mesenchymal transition, cell cycle and apoptosis-related markers, providing the possibility that ATP6V0D2 may be a novel biomarker for the therapeutic intervention of EC.
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http://dx.doi.org/10.1007/s10388-020-00735-8DOI Listing
October 2020

Structural Characterization and Antioxidant Activity of Polysaccharides from (Doll.) Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway.

Molecules 2019 Sep 16;24(18). Epub 2019 Sep 16.

Department of Pharmacy, Weifang Medical University, Weifang 261053, China.

The purpose of this study was to characterize the polysaccharides from (Doll.) Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice.

Methods: A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and H nuclear magnetic resonance (H NMR) spectroscopy were carried out to characterize AMC polysaccharides. The mechanism was investigated exploring d-galactose-induced aging mouse model. Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver.

Key Findings: Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw). AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated (), , (), (), and () mRNA expression, raised / ratio, downregulated mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression.

Conclusion: AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the pathway, which might in part contributed to their anti-aging activity.
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http://dx.doi.org/10.3390/molecules24183364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766938PMC
September 2019

Striatisporolide A, a butenolide metabolite from Athyrium multidentatum (Doll.) Ching, as a potential antibacterial agent.

Mol Med Rep 2019 Jul 15;20(1):198-204. Epub 2019 May 15.

Department of Pharmacy, Weifang Medical University, Weifang, Shandong 261053, P.R. China.

The present study aimed to investigate the antibacterial activity of striatisporolide A (SA) against Escherichia coli (E. coli) and the underlying mechanism. Antibacterial activity was evaluated according to the inhibitory rate and zone of inhibition. The antibacterial mechanism was investigated by analyzing alkaline phosphatase (AKP) activity and ATP leakage, protein expression, cell morphology and intracellular alterations in E. coli. The results demonstrated that SA exerted bacteriostatic effects on E. coli in vitro. AKP activity and ATP leakage analysis revealed that SA damaged the cell wall and cell membrane of E. coli. SDS‑PAGE analysis indicated that SA notably altered the level of 10 and 35 kDa proteins. Scanning electron microscopy and transmission electron microscopy analyses revealed marked alterations in the morphology and ultrastructure of E. coli following treatment with SA. The mechanism underlying the antimicrobial effects of SA against E. coli may be attributed to its actions of disrupting the cell membrane and cell wall and regulation of protein level. The findings of the present study provide novel insight into the antimicrobial activity of SA as a potential natural antibacterial agent.
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http://dx.doi.org/10.3892/mmr.2019.10244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579988PMC
July 2019

ASSOCIATION BETWEEN SERUM URIC ACID LEVEL AND BODY MASS INDEX IN SEX- AND AGE-SPECIFIC GROUPS IN SOUTHWESTERN CHINA.

Endocr Pract 2019 May 18;25(5):438-445. Epub 2019 Jan 18.

To investigate the sex- and age-specific association between serum uric acid level and body mass index (BMI). A total of 144,856 subjects aged 20 to 79 years were enrolled in this cross-sectional study. Serum uric acid level, renal function, hepatic function, and lipid profile were investigated. The prevalence of hyperuricemia decreased with age in men but increased in women. In men, the correlation coefficient between the serum urate level and BMI declined steadily with age. Underweight was associated with a 53 to 68% and a 66% lower prevalence of hyperuricemia in men aged 20 to 69 years and in women aged 20 to 29 years, respectively. Overweight and obesity were correlated with a higher odds ratio (OR) (95% confidence interval [CI]) for hyperuricemia in both genders. In individuals with overweight or obesity, younger subjects had a higher OR (95% CI) for hyperuricemia than older subjects. Among subjects aged 20 to 59 years, as they gained weight, the OR (95% CI) for hyperuricemia increased faster in women than in men compared with their respective normal-weight controls. Underweight was associated with a lower prevalence of hyperuricemia in men aged ≤69 years. In individuals with overweight or obesity, younger subjects were more likely to develop hyperuricemia than older subjects. With active weight gain, the likelihood for developing hyperuricemia increased faster in women than in men compared with their respective normal-weight controls. = alanine aminotransferase; = aspartate aminotransferase; = body mass index; = confidence interval; = estimated glomerular filtration rate; = high-density-lipoprotein cholesterol; = low-density-lipoprotein cholesterol; = odds ratio.
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http://dx.doi.org/10.4158/EP-2018-0426DOI Listing
May 2019

α-Glucosidase immobilization on chitosan-modified cellulose filter paper: Preparation, property and application.

Int J Biol Macromol 2019 Feb 26;122:298-305. Epub 2018 Oct 26.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. Electronic address:

In the present study, α-glucosidase (α-Glu) was immobilized on the chitosan-modified cellulose filter paper (CFP/CS). For α-Glu immobilization, glutaraldehyde (GA) was used as a coupling agent. CFP, environmentally friendly and commercially available with low cost, will avoid the tedious procedure for synthesizing immobilization carrier. In addition, the CFP/CS-immobilized α-Glu can be directly taken out from the reaction mixture after an enzymatic reaction. This makes the instantaneous separation of immobilized enzyme comes true and is convenient to the subsequent study. Combined with capillary electrophoresis (CE), the CFP/CS-immobilized α-Glu was then used for enzyme kinetic and inhibition study. The CFP/CS-immobilized α-Glu exhibited enhanced pH and temperature tolerance. In addition, the performance of the CFP/CS-immobilized α-Glu was studied. Immobilized α-Glu exhibited excellent batch-to-batch reproducibility (RSD = 6.7%, n = 5) and improved reusability (71.0% of its initial activity after 10 repeated cycles). Finally, immobilized α-Glu was used to screen enzyme inhibitors from 11 traditional Chinese medicines (TCMs). The results showed that CFP/CS has potential development prospects as a novel enzyme immobilization carrier.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.10.177DOI Listing
February 2019

Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability.

Anal Chim Acta 2018 May 30;1006:90-98. Epub 2017 Dec 30.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

In the present study, the immobilization method of physical adsorption combined with covalent crosslinking was developed to avoid the shortcomings of both the noncovalent and covalent coupling methods. For the first time, tyrosinase (TYR) was immobilized on the surface of aminated magnetic nanoparticles (FeO-NH) by the developed method. TYR was firstly adsorbed on the surface of FeO-NH by electrostatic interaction, and then by covalent crosslinking with glutaraldehyde (GA), TYR was firmly immobilized on the supports. The immobilized TYR showed enhanced pH and temperature endurances at the optimum pH of 7.0 and temperature of 35 °C. TYR reusability was significantly improved after immobilization and it retained 61.4 ± 2.3% of its initial activity after 5 repeated cycles. Immobilized TYR also showed improved storage stability (73.2 ± 1.1% after 30 days of storage at 4 °C). In addition, the immobilized TYR showed a higher biological affinity to substrate owing to the stabilization of TYR in its active conformation by electrostatic interaction prior to covalent crosslinking. Finally, the immobilized TYR was used to screen of enzyme inhibitors from 11 traditional Chinese medicines (TCMs) to validate whether this method can be used for enzyme inhibitor screening or not.
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http://dx.doi.org/10.1016/j.aca.2017.12.022DOI Listing
May 2018

Bone: Another potential target to treat, prevent and predict diabetes.

Diabetes Obes Metab 2018 Aug 14;20(8):1817-1828. Epub 2018 May 14.

Department of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-jin Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai, China.

Type 2 diabetes mellitus is now a worldwide health problem with increasing prevalence. Mounting efforts have been made to treat, prevent and predict this chronic disease. In recent years, increasing evidence from mice and clinical studies suggests that bone-derived molecules modulate glucose metabolism. This review aims to summarize our current understanding of the interplay between bone and glucose metabolism and to highlight potential new means of therapeutic intervention. The first molecule recognized as a link between bone and glucose metabolism is osteocalcin (OCN), which functions in its active form, that is, undercarboxylated OCN (ucOC). ucOC acts in promoting insulin expression and secretion, facilitating insulin sensitivity, and favouring glucose and fatty acid uptake and utilization. A second bone-derived molecule, lipocalin2, functions in suppressing appetite in mice through its action on the hypothalamus. Osteocytes, the most abundant cells in bone matrix, are suggested to act on the browning of white adipose tissue and energy expenditure through secretion of bone morphogenetic protein 7 and sclerostin. The involvement of bone resorption in glucose homeostasis has also been examined. However, there is evidence indicating the implication of the receptor activator of nuclear factor κ-B ligand, neuropeptide Y, and other known and unidentified bone-derived factors that function in glucose homeostasis. We summarize recent advances and the rationale for treating, preventing and predicting diabetes by skeleton intervention.
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http://dx.doi.org/10.1111/dom.13330DOI Listing
August 2018

Stereodirecting Effect of C5-Carboxylate Substituents on the Glycosylation Stereochemistry of 3-Deoxy-d- manno-oct-2-ulosonic Acid (Kdo) Thioglycoside Donors: Stereoselective Synthesis of α- and β-Kdo Glycosides.

J Am Chem Soc 2018 03 6;140(10):3574-3582. Epub 2018 Mar 6.

Department of Chemistry of Medicinal Natural Products, Sichuan Engineering Laboratory for Plant-Sourced Drug and Research Center for Drug Industrial Technology, West China School of Pharmacy, and State Key Laboratory of Biotherapy , West China Hospital, Sichuan University , Chengdu 610041 , China.

The stereodirecting effect of C5-ester functions on the glycosylation stereoselectivity of 3-deoxy-d- manno-oct-2-ulosonic acid (Kdo) ethyl thioglycoside donors is presented. The coupling of 5- O-arylcarbonyl or acetyl protected Kdo thioglycosides with acceptors proceeds in an α-selective and high-yielding manner, leading to formation of α-linked Kdo glycosides products. On the other hand, the glycosylation stereoselectivity of the 5- O-2-quinolinecarbonyl (Quin) or 4-nitropicoloyl substituted Kdo thioglycoside donors is switchable: (1) The glycosylation of the 5- O-Quin carrying Kdo donors with primary glycosyl acceptors shows complete β-stereoselectivity, furnishing the corresponding β-glycosides in good-to-excellent yield. (2) The stereochemical outcome of the secondary acceptors with these Kdo donors is determined mainly by the stereoelectronic nature of the acceptor. Only or predominant α anomeric products are obtained when the Kdo donors couple with the disarmed or highly crowded secondary carbohydrate acceptors, while the selectivity may switch to predominant β in the glycosylation of the 5- O-4-nitropicoloyl carrying donor with more reactive secondary alcohols. The synthetic use of the newly developed Kdo donors 1c and 7b has been demonstrated by facile preparation of a structurally unique trisaccharide motif 19 which possesses both α- and β-Kdo glycosidic bonds.
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http://dx.doi.org/10.1021/jacs.7b09461DOI Listing
March 2018

[Serological survey of infection in women with adverse pregnancy outcomes in Longhua region, Hebei Province].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2017 Mar;29(2):216-218

Longhua County Maternal and Child Health Care Center, Hebei Province, China.

Objective: To understand the status of infection in women with adverse pregnancy outcomes in Longhua region, Hebei Province.

Methods: A total of 393 women with adverse pregnancy outcomes were chosen as respondents in the Longhua County Maternal and Child Health Care Center between January 2013 and June 2016 and were divided into an acute infection group, a previous infection group, and an active infection group according to the test results. Totally 256 women without adverse pregnancy outcomes were selected as a control group. IgM and IgG antibodies to were detected by using ELISA in each group. The risk factors of infection were surveyed by questionnaires.

Results: The infection rate of the women with adverse pregnancy outcomes was 27.23% (107/393), which was significantly higher than 8.20% (21/ 256) in the control group ( = 35.46, <0.01). The rates of acute infection, previous infection, active infection in the women with adverse pregnancy outcomes were 6.87% (27/393), 18.58% (73/393), and 2.54% (10/393) respectively, which was significantly higher than those[1.17% (3/256), 7.03% (18/256), 0 (0/256) ]in the control group ( = 11.43, 17.15, 7.90 respectively, <0.01). The ratios of the career in contact with raw meat, feeding pets (dog and cat), tasting raw meat, chopping board regardless of uncooked or cooked food, frequently eating rinsing boiler or barbecue, frequently eating outside of the women with infection were significantly higher than those of the women without infection ( =12.08, 29.23, 8.55, 13.41, 7.28, 6.06 respectively, < 0.01 or 0.05).

Conclusions: infection could lead to serious adverse pregnancy outcomes. Therefore, to avoid contacting with pets, not eating undercooked food, and strengthening personal health protection are the important keys to avoid infection.
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http://dx.doi.org/10.16250/j.32.1374.2016194DOI Listing
March 2017

The relationship among serum lipocalin 2, bone turnover markers, and bone mineral density in outpatient women.

Endocrine 2018 02 2;59(2):304-310. Epub 2018 Jan 2.

Department of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-jin Hospital, Shanghai Jiao-tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai, 200025, China.

Purpose: We aimed to investigate associations among serum levels of LCN2, bone resorption marker carboxy-terminal cross-linking telopeptide of type-1 collagen (CTx), bone formation marker osteocalcin (OCN), and bone mineral densities (BMDs) in ambulatory healthy women.

Methods: This cross-sectional study analyzed 1012 previously enrolled outpatient Han Chinese women. BMDs of the lumbar spine and femoral neck were measured using dual energy X-ray absorptiometry. Serum levels of LCN2, CTx, OCN, and creatinine (Scr) were measured.

Results: Circulating LCN2 was inversely correlated with BMDs at the lumbar spine and femoral neck (Spearman's r = -0.08, P = 0.010 and r = -0.14, P < 0.001; respectively). A significant positive correlation between LCN2 and CTx (r = 0.11, P < 0.001), OCN (r = 0.06, P = 0.047), age (r = 0.21, P < 0.001), and Scr (r = 0.24, P < 0.001) was also observed. After adjusting for age and Scr, the correlation among LCN2, BMDs and OCN disappeared, but LCN2 was still positively associated with CTx (r = 0.08, P = 0.010). The circulating concentration of LCN2 showed no significant difference between subjects with and without osteoporotic fractures (43.63 (35.29, 53.66) vs. 42.25 (34.43, 51.46) ng/ml, respectively, P = 0.111). Serum CTx concentrations rose with serum LCN2 increasing from the lowest to the highest quartile (P for trend = 0.005), even after adjusting for age and Scr (P for trend = 0.040). In multivariate regression analysis, LCN2 was one of the main determinants for changes in serum CTx (standard β = 0.061, P = 0.005).

Conclusions: In ambulatory healthy women, the relationships among serum LCN2 level, BMDs, and OCN were confounded by age and Scr. Although LCN2 was positively related with CTx, the correlation was very weak and may not be physiologically relevant.
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http://dx.doi.org/10.1007/s12020-017-1504-1DOI Listing
February 2018

α-Glucosidase immobilization on chitosan-enriched magnetic composites for enzyme inhibitors screening.

Int J Biol Macromol 2017 Dec 16;105(Pt 1):308-316. Epub 2017 Jul 16.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

In the present study, the chitosan-enriched magnetic composites (MCCs) were prepared by a novel and simple embedding method for the immobilization of α-glucosidase (α-Glu). The immobilized α-Glu could be easily separated from the reaction mixture under an external magnetic field owing to the magnetic support. With the MCCs-immobilized α-Glu, enzyme activity and stability were studied, and enzyme inhibitors were screened from traditional Chinese medicines (TCMs) and vegetables combined with capillary electrophoresis (CE). The MCCs-immobilized α-Glu exhibited enhanced pH and temperature tolerance with unchanged optimum pH and temperature of 4.0 and 60°C comparing with free α-Glu. Reusability of the immobilized α-Glu was significantly improved after immobilization, and it retained 62.2% of its initial activity after 10 repeated cycles. Immobilized α-Glu also showed improved storage stability (84.3±1.2% after 35days of storage at 4°C). The kinetic parameter K for immobilized α-Glu was calculated to be 0.81mM and the affinity of enzyme towards its substrate was reduced after immobilization. Finally, immobilized α-Glu was used to screen enzyme inhibitors from the extracts of TCMs and vegetables. The enhanced pH and temperature tolerance, improved reusability and storage stability of MCCs-immobilized α-Glu make it a promising candidate for biotechnological applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2017.07.045DOI Listing
December 2017

The association between the baseline bone resorption marker CTX and incident dysglycemia after 4 years.

Bone Res 2017 4;5:17020. Epub 2017 Jul 4.

Department of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai, China.

Bone is an endocrine organ involved in modulating glucose homeostasis. The role of the bone formation marker osteocalcin (OCN) in predicting diabetes was reported, but with conflicting results. No study has explored the association between baseline bone resorption activity and incident diabetes or prediabetes during follow-up. Our objective was to examine the relationship between the baseline bone resorption marker crosslinked C-telopeptide of type I collagen (CTX) and glycemic dysregulation after 4 years. This longitudinal study was conducted in a university teaching hospital. A total of 195 normal glucose tolerant (NGT) women at baseline were invited for follow-up. The incidence of diabetes and prediabetes (collectively defined as dysglycemia) was recorded. A total of 128 individuals completed the 4-year study. The overall conversion rate from NGT to dysglycemia was 31.3%. The incidence of dysglycemia was lowest in the middle tertile [16.3% (95% confidence interval (CI), 6.8%-30.7%)] compared with the lower [31.0% (95% CI, 17.2%-46.1%)] and upper [46.5% (95% CI, 31.2%-62.6%)] tertiles of CTX, with a significant difference seen between the middle and upper tertiles (=0.002 5). After adjusting for multiple confounding variables, the upper tertile of baseline CTX was associated with an increased risk of incident dysglycemia, with an odds ratio of 7.09 (95% CI, 1.73-28.99) when the middle tertile was the reference. Osteoclasts actively regulate glucose homeostasis in a biphasic model that moderately enhanced bone resorption marker CTX at baseline provides protective effects against the deterioration of glucose metabolism, whereas an overactive osteoclastic function contributes to an increased risk of subsequent dysglycemia.
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http://dx.doi.org/10.1038/boneres.2017.20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496471PMC
July 2017

The pathological roles of NDRG2 in Alzheimer's disease, a study using animal models and APPwt-overexpressed cells.

CNS Neurosci Ther 2017 Aug 2;23(8):667-679. Epub 2017 Jul 2.

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Department of Pharmacology, Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Aims: To investigate the roles of N-myc downstream-regulated gene 2 (NDRG2) in the pathology of aging and neurodegenerative disease such as Alzheimer's disease (AD).

Results: In this study, we confirmed the upregulation of NDRG2 in the brains of aging and AD animal models. To explore the role of NDRG2 in the pathology of AD at molecular level, we conducted a cell-based assay of highly expressed wild-type human APP695 SK-N-SH cells (SK-N-SH APPwt). By silencing and overexpressing gene of NDRG2, we demonstrated that NDRG2-mediated increase in Aβ was through the pathways of BACE1 and GGA3. NGRG2 improved tau phosphorylation via enhanced activity of CDK5 and decreased Pin1, but it was not affected by GSK3β pathway. NDRG2 might also induce cell apoptosis through the extrinsic (caspase 8) apoptotic pathway by interaction with STAT3.

Conclusion: Our study confirmed the upregulation of NDRG2 in AD animal models and demonstrated its important roles in AD pathology. NDRG2 might be a potential target for studying and treatment of AD.
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http://dx.doi.org/10.1111/cns.12716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492714PMC
August 2017

Attenuation of deregulated miR-369-3p expression sensitizes non-small cell lung cancer cells to cisplatin via modulation of the nucleotide sugar transporter SLC35F5.

Biochem Biophys Res Commun 2017 07 13;488(3):501-508. Epub 2017 May 13.

Department of Oncology, First Hospital of Yulin City, Yulin 719000, PR China; Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China. Electronic address:

Deregulation of the microRNAs (miRNAs), a cluster of important posttranscriptional regulators, has been frequently associated with lung cancer (LCa). However, the emerging mechanism for how miRNAs is linked causally in the development of LCa chemoresistance is poorly understood. Herein, we established for the time the up-regulation of miR-369-3p in cisplatin (DDP)-resistant nonsmall cell lung cancer (NSCLC) tissues and cells. Its deregulation was found to be correlated to the magnitude of malignancy in well-characterized LCa cells. Functionally, inhibition of miR-369-3p sensitized LCa cells to DDP and suppressed the invasive capability in the presence of DDP treatment, whereas miR-369-3p overexpression promoted DDP resistance and thereby enhanced LCa cells invasiveness. Mechanistically, bioinformatics coupled with luciferase and gain-of-function, loss-of-function assays revealed that miR-369-3p may regulate DDP chemoresistance by directly targeting the 3' untranslated region (UTR) of human solute carrier 35F5 (SLC35F5), as application of miR-369-3p inhibitors or reintroduction of epigenetically silenced SLC35F5 both individually sensitized LCa cells to DDP, but combined treatment with miR-369-3p inhibitors and SLC35F5 overexpression failed to sensitized LCa cells further to DDP-elicited cell death. Our results provide evidence that the oncomiR effect of miR-369-3p may be mediated through disrupting the nucleotide sugar transportation and that SLC35F5 is a key effector of this chemoresistance-promoting activity.
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http://dx.doi.org/10.1016/j.bbrc.2017.05.075DOI Listing
July 2017

Pseudoginsenoside-F11 attenuates cerebral ischemic injury by alleviating autophagic/lysosomal defects.

CNS Neurosci Ther 2017 Jul 9;23(7):567-579. Epub 2017 May 9.

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China.

Aims: Pseudoginsenoside-F11 (PF11), an ocotillol-type ginsenoside, has been reported to exert wide-ranging neuroprotective properties. The aim of this study was to investigate the effect and potential mechanisms of PF11 on the autophagic/lysosomal pathway following ischemic stroke.

Methods: Male Sprague-Dawley rats underwent permanent middle cerebral artery occlusion (pMCAO). Cerebral ischemia outcome, TUNEL staining, Fluoro-Jade B staining were carried out 24 hours poststroke. The autophagic/lysosomal-related proteins were measured.

Results: A single administration of PF11 significantly decreased the infarct area, reduced the brain water content, and improved neurological functions, even 4 hours after the onset of pMCAO. Meanwhile, PF11 lessened the ischemic insult-mediated loss of neurons and activation of astrocytes and microglia. Furthermore, PF11 attenuated pMCAO-induced accumulations of autophagosomes and apoptosis. We further observed a remarkable effect of PF11 in reversing the ischemic insult-induced accumulation of autophagosomes (LC3-II) and abnormal aggregation of autophagic proteins (SQSTM1 and ubiquitin). Furthermore, PF11 was capable of improving lysosomal function and lysosome/autophagosome fusion following pMCAO, and this change was reversed by the lysosomal inhibitor chloroquine. Also, the improvement of ischemic outcome and the antiapoptotic effect induced by PF11 was reversed by CQ.

Conclusion: These findings indicate that the autophagic flux is impaired in a rat model of pMCAO, and that PF11 exerts an excellent protective effect against ischemic stroke by alleviating autophagic/lysosomal defects.
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http://dx.doi.org/10.1111/cns.12702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492733PMC
July 2017

Endovascular Treatment of an Unusual Membranous Obstruction of the Inferior Vena Cava in Budd-Chiari Syndrome Complicated by Mural Portal Vein Thrombosis.

Ann Vasc Surg 2017 Oct 5;44:419.e13-419.e17. Epub 2017 May 5.

Department of Interventional Radiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

We describe the case of a patient with Budd-Chiari syndrome who presented with an unusual membranous obstruction of the inferior vena cava complicated by massive portal vein thrombosis (PVT). The patient underwent percutaneous transluminal balloon angioplasty through the right groin and was prescribed oral warfarin for 6 months. Treatment resulted in the complete disappearance of the PVT. This therapeutic strategy should be considered in the management of other cases of this rare, complex disease.
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http://dx.doi.org/10.1016/j.avsg.2017.04.026DOI Listing
October 2017

Kinetics and inhibition study of tyrosinase by pressure mediated microanalysis.

Anal Biochem 2017 05 1;525:54-59. Epub 2017 Mar 1.

Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

In the present study, pressure mediated microanalysis (PMMA), a fast, convenient and efficient capillary electrophoresis (CE) method was developed for studying enzyme kinetics of tyrosinase and inhibition kinetics of kojic acid, a model inhibitor of tyrosinase. The enzymatic reaction conditions and CE conditions were optimized in order to obtain high enzyme activity and short analysis time. By PMMA, only the product could be detected at 475 nm, and no voltage was applied to separate the product from the reaction mixture thus greatly simplifying the optimization procedure. The spectrophotometric assay and electrophoretically mediated microanalysis (EMMA) were also performed to validate the developed method. With the present method, the Michaelis-Menten constant (K) was calculated to be 1.347 mM for tyrosinase. The inhibition constant of kojic acid to free tyrosinase (K) and kojic acid to tyrosinase/L-DOPA complex (K) were calculated to be 36.64 and 74.35 μM, respectively, and the half-maximal inhibitory concentration (IC) was determined to be 46.64 μM for kojic acid. The developed method is fast and convenient for studying enzyme kinetics, inhibition kinetics and further screening enzyme inhibitors.
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http://dx.doi.org/10.1016/j.ab.2017.02.020DOI Listing
May 2017
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