Publications by authors named "Dong-Mei Wei"

30 Publications

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Identification of maternal continuous glucose monitoring metrics related to newborn birth weight in pregnant women with gestational diabetes.

Endocrine 2021 Jun 14. Epub 2021 Jun 14.

Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

Purpose: To identify the specific glucose metrics derived from maternal continuous glucose monitoring (CGM) data, which were associated with a higher percentile of offspring birth weight.

Methods: In this cohort study, we recruited singleton pregnant women with GDM who underwent CGM for 5-14 days at a mean of 28.8 gestational weeks between Jan 2017 and Nov 2018. Commonly used single summary glucose metrics of glucose exposure (including mean 24-h, daytime, and nighttime glucose level) and variability (including J-index and mean amplitude of glycaemic excursions) were derived from CGM data. A novel comprehensive glucose metric-hours per-day spent in a severe variability glucose mode (HSSV)-was identified using the spectral clustering method, which reflects both glucose level and variability. Multiple linear regression models were used to estimate the associations of sex- and gestational age-adjusted birth weight percentile with CGM parameters.

Results: Ninety-seven women comprising 127,279 glucose measurements were included. Each 1-SD increase in maternal nighttime mean glucose level and HSSV was associated with 6.0 (95% CI 0.4, 11.5) and 6.3 (95% CI 0.4, 12.2) percentage points increase in birth weight percentile, respectively. No associations were found between other glucose metrics and birth weight percentile.

Conclusion: Nighttime mean glucose level has a comparable effect size to HSSV in association with fetal growth, suggesting that endogenous hyperglycemia might drive the association between maternal hyperglycemia and birth weight. Further studies need to examine the effect of lowering nighttime glucose level and/or HSSV on preventing fetal overgrowth in GDM women.
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http://dx.doi.org/10.1007/s12020-021-02787-xDOI Listing
June 2021

Two-Year Responses of Heart Rate and Heart Rate Variability to First Occupational Lead Exposure.

Hypertension 2021 May 29;77(5):1775-1786. Epub 2021 Mar 29.

From the Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Sciences, University of Leuven, Belgium (Y.-L.Y., L.T., J.D.M., D.-M.W., Z.-Y.Z.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.16545DOI Listing
May 2021

Relative and Absolute Risk to Guide the Management of Pulse Pressure, an Age-Related Cardiovascular Risk Factor.

Am J Hypertens 2021 Mar 4. Epub 2021 Mar 4.

Laboratory of Neurosciences, Faculty of Medicine, University of Zulia, Maracaibo, Zulia, Venezuela.

Background: Pulse pressure (PP) reflects the age-related stiffening of the central arteries, but no study addressed the management of the PP-related risk over the human lifespan.

Methods: In 4663 young (18-49 years) and 7185 older adults (≥50 years), brachial PP was recorded over 24-hour. Total mortality and all major cardiovascular events combined (MACE) were co-primary endpoints. Cardiovascular death, coronary events and stroke were secondary endpoints.

Results: In young adults (median follow-up, 14.1 years; mean PP, 45.1 mmHg), greater PP was not associated with absolute risk; the endpoint rates were ≤2.01 per 1000 person-years. The adjusted hazard ratios expressed per 10mmHg PP increments were less than unity (P≤0.027) for MACE (0.67; 95% CI, 0.47-0.96) and cardiovascular death (0.33; 95% CI, 0.11-0.75). In older adults (median follow-up, 13.1 years; mean PP, 52.7 mmHg), the endpoint rates, expressing absolute risk, ranged from 22.5 to 45.4 per 1000 person-years and the adjusted hazard ratios, reflecting relative risk, from 1.09 to 1.54 (P<0.0001). The PPrelated relative risks of death, MACE and stroke decreased >3-fold from age 55 to 75 years, whereas absolute risk rose by a factor 3.

Conclusions: From 50 years onwards, the PP-related relative risk decreases, whereas absolute risk increases. From a lifecourse perspective, young adulthood provides a window of opportunity to manage risk factors and prevent target organ damage as forerunner of premature death and MACE. In older adults, treatment should address absolute risk, thereby extending life in years and quality.
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http://dx.doi.org/10.1093/ajh/hpab048DOI Listing
March 2021

Two-year neurocognitive responses to first occupational lead exposure.

Scand J Work Environ Health 2021 Apr 4;47(3):233-243. Epub 2020 Dec 4.

Research Institute Alliance for the Promotion of Preventive Medicine, Leopoldstraat 59, BE-2800 Mechelen, Belgium.

Objectives Lead exposure causes neurocognitive dysfunction in children, but its association with neurocognition in adults at current occupational exposure levels is uncertain mainly due to the lack of longitudinal studies. In the Study for Promotion of Health in Recycling Lead (NCT02243904), we assessed the two-year responses of neurocognitive function among workers without previous known occupational exposure newly hired at lead recycling plants. Methods Workers completed the digit-symbol test (DST) and Stroop test (ST) at baseline and annual follow-up visits. Blood lead (BL) was measured by inductively coupled plasma mass spectrometry (detection limit 0.5 µg/dL). Statistical methods included multivariable-adjusted mixed models with participants modelled as random effect. Results DST was administered to 260 participants (11.9% women; 46.9%/45.0% whites/Hispanics; mean age 29.4 years) and ST to 168 participants. Geometric means were 3.97 and 4.13 µg/dL for baseline BL, and 3.30 and 3.44 for the last-follow-up-to-baseline BL ratio in DST and ST cohorts, respectively. In partially adjusted models, a doubling of the BL ratio was associated with a 0.66% [95% confidence interval (CI) 0.03-1.30; P=0.040] increase in latency time (DST) and a 0.35% (95% CI ‑1.63-1.63; P=0.59) decrease in the inference effect (ST). In fully adjusted models, none of the associations of the changes in the DST and ST test results with the blood lead changes reached statistical significance (P≥0.12). Conclusions An over 3-fold increase in blood lead over two years of occupational exposure was not associated with a relevant decline in cognitive performance.
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http://dx.doi.org/10.5271/sjweh.3940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126443PMC
April 2021

Spinal Epidural Hematoma After Percutaneous Kyphoplasty: Case Report and Literature Review.

J Pain Res 2020 3;13:2799-2804. Epub 2020 Nov 3.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Objective: To present the case of a patient on long-term anticoagulants who developed acute spinal epidural hematoma (SEH) after percutaneous kyphoplasty (PKP) without signs of major cement extravasation to the spinal canal.

Methods: A 64-year-old woman with long-term oral antiplatelet drugs underwent the L1 PKP. Immediately after the operation, the back pain improved significantly without neurological deficit. However, 12 hours later, she developed progressive weakness of the bilateral lower limbs. No intraspinal cement leakage was obvious on the postoperative lumbar radiograph and computed tomography.

Results: An emergency MRI examination revealed a high signal aggregation in front of the spinal cord from T12 to L1, indicating spinal cord compression. The SEH was verified and removed during the laminectomy from T12-L1. Following the decompression surgery, the neurological deficit of the lower limbs improved. On follow-up after 6 months, the muscle strength of the bilateral lower limbs had returned to normal.

Conclusion: For the patient with long-term oral antiplatelet drugs or coagulation malfunction, the transpedicle approach or that via the costovertebral joint with a smaller abduction angle is recommended to reduce the risk of injury to the inner wall of the pedicle. For progressive aggravation of neurological dysfunction after surgery, SEH formation should be suspected despite the absence of intraspinal bone cement leakage. Secondary emergency decompression should be considered to avoid permanent damage to spinal cord nerve function caused by continuous compression.
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http://dx.doi.org/10.2147/JPR.S280650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648559PMC
November 2020

Two-Year Responses of Office and Ambulatory Blood Pressure to First Occupational Lead Exposure.

Hypertension 2020 10 9;76(4):1299-1307. Epub 2020 Sep 9.

From the Studies Coordinating Centre, Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Sciences, University of Leuven, Belgium (Y.-L.Y., L.T., J.D.M., C.-G.Y., D.-M.W., F.-F.W., Z.-Y.Z., J.A.S.).

Lead exposure causing hypertension is the mechanism commonly assumed to set off premature death and cardiovascular complications. However, at current exposure levels in the developed world, the link between hypertension and lead remains unproven. In the Study for Promotion of Health in Recycling Lead (URL: https://www.clinicaltrials.gov; Unique identifier: NCT02243904), we recorded the 2-year responses of office blood pressure (average of 5 consecutive readings) and 24-hour ambulatory blood pressure to first occupational lead exposure in workers newly employed at lead recycling plants. Blood lead (BL) was measured by inductively coupled plasma mass spectrometry (detection limit 0.5 µg/dL). Hypertension was defined according to the 2017 American College of Cardiology/American Heart Association guideline. Statistical methods included multivariable-adjusted mixed models with participants modeled as a random effect and interval-censored Cox regression. Office blood pressure was measured in 267 participants (11.6% women, mean age at enrollment, 28.6 years) and ambulatory blood pressure in 137 at 2 follow-up visits. Geometric means were 4.09 µg/dL for baseline BL and 3.30 for the last-follow-up-to-baseline BL ratio. Fully adjusted changes in systolic/diastolic blood pressure associated with a doubling of the BL ratio were 0.36/0.28 mm Hg (95% CI, -0.55 to 1.27/-0.48 to 1.04 mm Hg) for office blood pressure and -0.18/0.11 mm Hg (-2.09 to 1.74/-1.05 to 1.27 mm Hg) for 24-hour ambulatory blood pressure. The adjusted hazard ratios of moving up across hypertension categories for a doubling in BL were 1.13 (0.93-1.38) and 0.84 (0.57-1.22) for office blood pressure and ambulatory blood pressure, respectively. In conclusion, the 2-year blood pressure responses and incident hypertension were not associated with the BL increase on first occupational exposure.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.15590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480942PMC
October 2020

Target-modulated sensitization of upconversion luminescence by NIR-emissive quantum dots: a new strategy to construct upconversion biosensors.

Chem Commun (Camb) 2020 Feb 21;56(13):1976-1979. Epub 2020 Jan 21.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

We herein used AgSe quantum dots (QDs) as a target-modulated sensitizer for upconversion nanoparticles (UCNPs) and the target thrombin as the sensitizing switch to construct a biosensor, circumventing the limited luminescence resonance energy transfer (LRET) efficiency of UCNPs, with enhanced signal-to-background ratio (SBR) and assay sensitivity.
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http://dx.doi.org/10.1039/c9cc09220jDOI Listing
February 2020

[A Study on the Risk Factors for Early Postpartum Urinary Incontinence in Chengdu].

Sichuan Da Xue Xue Bao Yi Xue Ban 2019 Jul;50(4):598-603

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China.

Objective: To investigate the incidence of early postpartum urinary incontinence in parturients from Chengdu, and to find out the high-risk factors for reference for clinical diagnosis and treatment.

Methods: A total of 9 918 parturient women who gave delivery at the West China Second University Hospital of Sichuan University from January 2014 to January 2018 were enrolled and reviewed 6 weeks after delivery. The prevalence of urinary incontinence at 6 weeks postpartum was investigated by questionnaire. χ test and multivariate logistic regression analysis were used to analyze the risk factors affecting the prevalence.

Results: 9 550 parturient women were actually investigated. The prevalence of urinary incontinence was 15.53% (1 483/9 550) at 6 weeks postpartum in Chengdu, among which stress urinary incontinence was the most common (73.03%, 1 083/1 483). Univariate analysis showed that age, pelvic surgery history, prenatal body mass index (BMI), urinary incontinence during pregnancy, neonatal body mass, the number of parturition, delivery mode, lateral perineal incision, perineal laceration and prolonged second stage of labor were all correlated with the occurrence of urinary incontinence at 6 weeks postpartum ( < 0.05). Multivariate logistic regression analysis showed that cesarean section can reduce the risk of urinary incontinence compared with vaginal delivery 〔odds ratio ()=0.373, < 0.001〕. Age≥35 yr. (=1.803, =0.001), pelvic surgery history (=1.260, =0.003), BMI≥28 kg/m during pregnancy (=1.694, =0.025), urinary incontinence during pregnancy (=2.605, < 0.001), neonatal body mass ≥4 kg (=2.307, =0.040), multipara (=1.284, =0.023) and perineal laceration (=1.372, =0.035) were independent risk factors for urinary incontinence at 6 weeks postpartum.

Conclusions: Urinary incontinence at 6 weeks postpartum is not rare in Chengdu, and stress urinary incontinence is more frequent. Eutocia, elderly parturient, multipara, pelvic surgery history, prenatal obesity, urinary incontinence during pregnancy, large neonatal body mass and perineal laceration are the main risk factors for urinary incontinence at 6 weeks postpartum.
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July 2019

Family socioeconomic position and abnormal birth weight: evidence from a Chinese birth cohort.

World J Pediatr 2019 Oct 8;15(5):483-491. Epub 2019 Jul 8.

Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 9 Jinsui Road, Zhujiang Newtown, Tianhe District, Guangzhou 510623, China.

Background: Birth weight is a strong determinant of infant short- and long-term health outcomes. Family socioeconomic position (SEP) is usually positively associated with birth weight. Whether this association extends to abnormal birth weight or there exists potential mediator is unclear.

Methods: We analyzed data from 14,984 mother-infant dyads from the Born in Guangzhou Cohort Study. We used multivariable logistic regression to assess the associations of a composite family SEP score quartile with macrosomia and low birth weight (LBW), and examined the potential mediation effect of maternal pre-pregnancy body mass index (BMI) using causal mediation analysis.

Results: The prevalence of macrosomia and LBW was 2.62% (n = 392) and 4.26% (n = 638). Higher family SEP was associated with a higher risk of macrosomia (OR 1.30, 95% CI 0.93-1.82; OR 1.53, 95% CI 1.11-2.11; and OR 1.59, 95% CI 1.15-2.20 for the 2nd, 3rd, and 4th SEP quartile respectively) and a lower risk of LBW (OR 0.69, 95% CI 0.55-0.86; OR 0.76, 95% CI 0.61-0.94; and OR 0.61, 95% CI 0.48-0.77 for the 2nd, 3rd, and 4th SEP quartile respectively), compared to the 1st SEP quartile. We found that pre-pregnancy BMI did not mediate the associations of SEP with macrosomia and LBW.

Conclusions: Socioeconomic disparities in fetal macrosomia and LBW exist in Southern China. Whether the results can be applied to other populations should be further investigated.
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http://dx.doi.org/10.1007/s12519-019-00279-7DOI Listing
October 2019

Enhanced Superdense Coding over Correlated Amplitude Damping Channel.

Entropy (Basel) 2019 Jun 16;21(6). Epub 2019 Jun 16.

College of Physics and Electronic Information, Gannan Normal University, Ganzhou 341000, China.

Quantum channels with correlated effects are realistic scenarios for the study of noisy quantum communication when the channels are consecutively used. In this paper, superdense coding is reexamined under a correlated amplitude damping (CAD) channel. Two techniques named as weak measurement and environment-assisted measurement are utilized to enhance the capacity of superdense coding. The results show that both of them enable us to battle against the CAD decoherence and improve the capacity with a certain probability. Remarkably, the scheme of environment-assisted measurement always outperforms the scheme of weak measurement in both improving the capacity and successful probability. These notable superiorities could be attributed to the fact that environment-assisted measurement can extract additional information from the environment and thus it performs much better.
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http://dx.doi.org/10.3390/e21060598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515104PMC
June 2019

[Analysis on mechanisms and medication rules of herbal prescriptions for nonalcoholic fatty liver disease based on methods of data mining and biological information].

Zhongguo Zhong Yao Za Zhi 2019 Apr;44(8):1689-1695

Science and Education Department,Ningbo Hospital of Traditional Chinese Medicine Ningbo 315012,China.

To explore the medication rules of herbal prescriptions for nonalcoholic fatty liver disease,and analyze the possible drug targets and interactions,in order to explore the mechanisms of the herbs. Randomized controlled trials of herbal prescriptions for treating nonalcoholic fatty liver disease were collected from CNKI,Wan Fang,VIP,Sino Med and PubMed databases. The properties,flavors and meridian tropism of herbs were analyzed by using systematic cluster analysis method with SPSS 19. 0 software. Subsequently,the association rules of herbs were analyzed by using Clementine 12. 0 software. Finally,the interactions between targets and relevant signaling pathways were analyzed by Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),Search Tool for the Retrieval of Interacting Genes/Proteins(STRING) and Kyoto Encyclopedia of Genes and Genomes(KEGG). In the 88 prescriptions screened out,the commonly used herbs were Salvia miltiorrhiza,Bupleurum chinense,Alisma orientale,and Crataegus pinnatifida,and the potential signaling pathways were PPAR signaling pathway and calcium signaling pathway. The results showed that the main effects of herbal prescriptions were to improve blood flow/clear blood stasis,clear heatiness/dampness,promote digestion and strengthen spleen. And its mechanism of action may be achieved through the regulation of PPAR signaling pathway and calcium signaling pathway.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20190110.001DOI Listing
April 2019

A School-Based Comprehensive Intervention for Childhood Obesity in China: A Cluster Randomized Controlled Trial.

Child Obes 2019 Feb/Mar;15(2):105-115. Epub 2018 Dec 19.

1 Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, China.

Background: A comprehensive approach involving both environmental and individual strategies offers opportunities to strengthen school-based interventions for childhood obesity.

Objectives: To evaluate a theory-based comprehensive intervention implemented within primary schools for childhood obesity in China.

Methods: A 1-year, cluster randomized controlled trial was conducted in twelve primary schools (7-11 years old) in Beijing, China. Environmental and individual factors were improved in the intervention group (6 schools, 930 children) to promote knowledge and behaviors related to energy balance. The control group (6 schools, 959 children) did not receive any intervention. The primary outcome was body mass index (BMI) and its Z-score at 12 months. Generalized linear mixed models were used controlling for the cluster effect of school.

Results: No significant differences were found between groups on BMI (0.07 kg/m [95% confidence interval (CI) -0.16 to 0.31, p = 0.54]) and its Z-score (0.02 [95% CI: -0.08 to 0.11, p = 0.73]) at 12 months. Self-reported knowledge, daily consumption of sugar-sweetened beverage, and frequency of moderate to vigorous physical activity improved at 12 months.

Conclusions: The intervention did not mitigate excess weight gain, but did improve children's knowledge and several behaviors related to energy balance.
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http://dx.doi.org/10.1089/chi.2018.0251DOI Listing
July 2020

The role of social support in family socio-economic disparities in depressive symptoms during early pregnancy: Evidence from a Chinese birth cohort.

J Affect Disord 2018 10 5;238:418-423. Epub 2018 Jun 5.

Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 9 Jinsui Road, Zhujiang Newtown, Tianhe District, Guangzhou, China; Department of Woman and Child Health Care, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No. 9 Jinsui Road, Zhujiang Newtown, Tianhe District, Guangzhou, China. Electronic address:

Background: Few studies have examined the association between a composite measure of family socio-economic position (SEP) and depressive symptoms among Chinese pregnant women, nor any potential underlying mechanisms which may be amenable to preventative interventions.

Methods: We investigated the association between a composite SEP measure and depressive symptoms during early pregnancy, and tested for mediation by social support and moderation by parity in the Born in Guangzhou Cohort Study (n = 12,382) using adjusted logistic regression and causal mediation analysis.

Results: In this population, 18.4% of women experienced depressive symptoms before the 20th gestational week. Compared with the highest quartile, the lowest SEP score quartile was associated with a higher risk of depressive symptoms (OR 1.50, 95% CI 1.31-1.71), which was more pronounced among multiparous women than nulliparous women (P for interaction <0.001). Social support mediated the association between SEP and depressive symptoms, with greater proportion mediated in nulliparous women (73.4% for the lowest SEP score quartile) than multiparous women (30.5%).

Limitations: Depressive symptoms were measured by Self-rated Depression Scale, which is not designed as a clinical diagnosis tool for depression. We only had information on perceived social support but not actual social support, although these two parameters were modestly correlated.

Conclusion: Lower SEP was associated with higher risk of depressive symptoms in pregnant women, driven by social support. And the association between SEP and depressive symptoms and mediation by social support were modified by parity. Whether this association extends to the post pregnancy period or is amenable to cost-effective interventions should be investigated in further studies.
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http://dx.doi.org/10.1016/j.jad.2018.06.014DOI Listing
October 2018

Probiotics for vulvovaginal candidiasis in non-pregnant women.

Cochrane Database Syst Rev 2017 11 23;11:CD010496. Epub 2017 Nov 23.

Department of Obstetrics and Gynecology, People's Hospital of Deyang City, No. 173, Tai Shan North Road, Deyang, Sichuan, China, 618000.

Background: Vulvovaginal candidiasis (VVC) is estimated to be the second most common form of infection after bacterial vaginosis. The ability of probiotics in maintaining and recovering the normal vaginal microbiota, and their potential ability to resist Candidas give rise to the concept of using probiotics for the treatment of VVC.

Objectives: To assess the effectiveness and safety of probiotics for the treatment of vulvovaginal candidiasis in non-pregnant women.

Search Methods: We searched the following databases to October 2017: Sexually Transmitted Infections Cochrane Review Group's Specialized Register, CENTRAL, MEDLINE, Embase and eight other databases. We searched in following international resources: World Health Organization International Clinical Trials Registry Platform, ClinicalTrials.gov, Web of Science and OpenGrey. We checked specialty journals, reference lists of published articles and conference proceedings. We collected information from pharmaceutical companies and experts in the field.

Selection Criteria: Randomized controlled trials (RCT) using probiotics, alone or as adjuvants to conventional antifungal drugs, to treat VVC in non-pregnant women. Trials recruiting women with recurrent VVC, coinfection with other vulvovaginal infections, diabetes mellitus, immunosuppressive disorders or taking immunosuppressant medication were ineligible for inclusion. Probiotics were included if they were made from single or multiple species and in any preparation type/dosage/route of administration.

Data Collection And Analysis: Two review authors independently assessed trials for eligibility and quality and extracted data. We resolved any disagreements through consensus. The quality of the evidence was assessed using the GRADE approach.

Main Results: Ten RCTs (1656 participants) met our inclusion criteria, and pharmaceutical industry funded none of these trials. All trials used probiotics as adjuvant therapy to antifungal drugs. Probiotics increased the rate of short-term clinical cure (risk ratio (RR) 1.14, 95% confidence interval (CI) 1.05 to 1.24, 695 participants, 5 studies, low quality evidence) and mycological cure (RR 1.06, 95% CI 1.02 to 1.10, 969 participants, 7 studies, low quality evidence) and decreased relapse rate at one month (RR 0.34, 95% CI 0.17 to 0.68, 388 participants, 3 studies, very low quality evidence). However, this effect did not translate into a higher frequency of long-term clinical cure (one month after treatment: RR 1.07, 95% CI 0.86 to 1.33, 172 participants, 1 study, very low quality evidence; three months after treatment: RR 1.30, 95% CI 1.00 to 1.70, 172 participants, one study, very low quality evidence) or mycological cure (one month after treatment: RR 1.26, 95% CI 0.93 to 1.71, 627 participants, 3 studies, very low quality evidence; three months after treatment: RR 1.16, 95% CI 1.00 to 1.35, 172 participants, one study, very low quality evidence). Probiotics use did not increase the frequency of serious (RR 0.80, 95% CI 0.22 to 2.94; 440 participants, 2 studies, low quality evidence). We found no eligible RCTs for outcomes as time to first relapse, need for additional treatment at the end of therapy, patient satisfaction and cost effectiveness.

Authors' Conclusions: Low and very low quality evidence shows that, compared with conventional treatment, the use of probiotics as an adjuvant therapy could increases the rate of short-term clinical and mycological cure and decrease the relapse rate at one month but this did not translate into a higher frequency of long-term clinical or mycological cure. Probiotics use does not seem to increase the frequency of serious or non-serious adverse events. There is a need for well-designed RCTs with standardized methodologies, longer follow-up and larger sample size.
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http://dx.doi.org/10.1002/14651858.CD010496.pub2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486023PMC
November 2017

[Effects of Cellular Density on the Induction of Suspension Globe of Ovarian Cancer Stem Cells].

Sichuan Da Xue Xue Bao Yi Xue Ban 2017 Sep;48(5):758-762

Department of Gynaecology and Obstetrics,West China Second University Hospital,Sichuan University,Chengdu 610041,China.

Objective: To determine the effect of cellular density on the separation and identification of cancer stem cells from human ovarian clear cell carcinoma cell line ES-2 and adenocarcinoma cell line A2780.

Methods: ES-2 and A2780 cells were cultured with human recombinant epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and bovine serum albumin and insulin in serum free medium. The cancer stem cells were obtained through serial passages. Changes in cell morphology,expressions of surface marker CD133 and CD44,and soft AGAR clone forming in the stem cells were examined under different cell density,either in serum-supplemented medium (SSM group) or in serum free medium (SFM group).

Results: Under the density of 2×10 mL,ES-2 cells survived in SFM,but did not form stem cells. When the density increased to 5×10 mL or 1×10 mL,ES-2 cells survived in SFM,proliferated and formed stem cells. Compared with adherent cells,the suspension globe of stem cells expressed high levels of CD133 and CD44 ( <0.05),with proliferation and clone forming ability after serial passages. The stem cell balls under the density of 5×10 mL had stronger ability of tumor formation. A2780 cells formed suspension globe under the density of 1×10 mL and 3×10 mL,but larger and more transparent balls were observed under the density of 3×10 mL density. No suspension globe was formed under the density of 5×10 mL. More CD133/CD44cells were detected by flow cytometry under the density of 3×10 mL,compared with that under the density of 1×10 mL ( <0.05). Tumor stem cells grew faster under the density of 3×10 mL.

Conclusion: The optimal density for identifying stem cells from human ovarian cancer is 5×10 mL for ES-2 and 3×10 mL for A2780,respectively.
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September 2017

Systematic review and meta-analysis of school-based obesity interventions in mainland China.

PLoS One 2017 14;12(9):e0184704. Epub 2017 Sep 14.

Institute of Child and Adolescent Health, Department of Child, Adolescent and Women's Health, School of Public Health, Peking University, Beijing, China.

Background: Numerous school-based interventions for childhood obesity have been emerging in mainland China in recent decades, but little is known about the effectiveness of such interventions. This study aims to assess the effectiveness of school-based interventions for childhood obesity conducted in mainland China.

Methods: A systematic search was undertaken in eight databases to identify both randomized and non-randomized controlled trials from January 1990 to December 2015 examining the effectiveness of school-based obesity interventions. A random effects meta-analysis was conducted assessing the impact of included interventions on (body mass index) BMI. The quality of each included studies were assessed using Effective Public Health Practice Project Quality Assessment Tool. A P value <0.05 (two-sided) was considered statistically significant.

Result: Of the seventy-six included studies, we found physical activity and health education were the two most common components of interventions. More treatment studies were effective compared with prevention studies (85.0% vs. 58.3%). Comprehensive interventions involving physical activity and health education appeared more effective than the physical activity only interventions in both obesity treatment and prevention studies. The meta-analyses showed comprehensive interventions involving physical activity and health education had larger effect on the change of BMI than physical activity only interventions (treatment studies: -1.80 kg/m2 (95% CI: -2.15,-1.44) vs. -0.91 kg/m2 (95% CI: -1.15,-0.67); prevention studies: -0.19 kg/m2 (95% CI: -0.27, -0.11) vs. +0.05 kg/m2 (95% CI: -0.04, +0.15)).

Conclusions: Comprehensive school-based interventions may assist in tackling the rising prevalence of childhood obesity in mainland China.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184704PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598996PMC
October 2017

Associations between maternal exposure to incense burning and blood pressure during pregnancy.

Sci Total Environ 2018 Jan 30;610-611:1421-1427. Epub 2017 Aug 30.

Division of Birth Cohort Study, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China; Department of Woman and Child Health Care, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China. Electronic address:

Incense burning is a popular practice in Asian and Arabic countries. Previous studies show that incense burning was associated with increased risks of adverse outcomes among non-pregnant population. However, very few studies explored its health effects among pregnant women, who are more susceptible to environmental stressor. We aimed to examine the association between incense burning at home and hypertensive disorders as well as blood pressure levels during pregnancy, using data from 10,563 pregnant women recruited in Born in Guangzhou Cohort Study, China between January 2013 and December 2015. Information on frequency and duration of exposure to incense burning were collected at early and late pregnancy using questionnaire. Data on outcome variables, including hypertensive disorders diagnosis and blood pressure levels at the final antenatal visit before delivery, were extracted from medical records. We used Poisson regression model and general linear model to examine the associations between incense exposure and the outcomes. We found incense use at early pregnancy was not significantly associated with outcomes. Pregnant women who frequently smelled the incense burning at late pregnancy was associated with higher risk of hypertensive disorders (relative risk, 1.84; 95% confidence interval, 1.14-2.98) and higher levels of blood pressure (1.6mmHg increase of systolic blood pressure; 95% confidence interval, 0.4-2.8mmHg) before delivery, compared to those did not burn incense. These associations tended to more evident among women without active and passive smoking. We did not observe significant dose-response relationship between exposure duration and the risk of hypertensive disorders. We firstly reported exposure to incense burning was associated with the risk of hypertensive disorders and blood pressure levels during pregnancy. Given hypertensive disorders in pregnancy are well-established risk factors for a variety of adverse outcomes and the incense burning is a modifiable factor, our finding may have important public health significance.
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http://dx.doi.org/10.1016/j.scitotenv.2017.08.134DOI Listing
January 2018

Does local ambient temperature impact children's blood pressure? A Chinese National Survey.

Environ Health 2016 Feb 16;15:21. Epub 2016 Feb 16.

Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing, China.

Background: Several studies demonstrated a short-term association between ambient temperature and blood pressure. However, few studies have assessed the long-term effect of ambient temperature on children's blood pressure. The present study aimed to investigate the association between long-term exposure to local ambient temperature and children's blood pressure in China.

Methods: We analyzed the systolic (SBP) and diastolic blood pressure (DBP) data of 71,763 children from 2010 Chinese National Survey on Students' Construction and Health (CHNSCH), and local annual average ambient temperature, relative humidity, air pollutants data from China Meteorological Administration and Ministry of Environment Protection of China. We used generalized additive model (GAM) with non-linear function to examine the effects of ambient temperature on children's blood pressure.

Results: The results showed that decrease of ambient temperature was negatively associated with increase of both SBP and DBP in Chinese children while adjusting for individual characteristics, socioeconomic conditions, air pollutants and relative humidity. The largest alteration of SBP related to the temperature difference was observed from 20.4 to 9.6 °C, with 9.0 mmHg (95 % CI: 8.4, 9.5) increase in SBP, while the largest alteration of DBP was observed from 21.7 to 10.2 °C, with 6.1 mmHg (95 % CI: 5.6, 6.6) increase in DBP. However, when temperature below 9.6 and 10.2 °C, SBP and DBP started to decrease, which might be caused by the use of heating system in the extreme cold areas.

Conclusions: Public health policy should be improved for protecting children's cardiovascular health from adverse effects of low temperature. Development of heating system in moderate cold area might be a good solution.
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http://dx.doi.org/10.1186/s12940-016-0119-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754880PMC
February 2016

Thrombomodulin and High-Sensitive C-Reactive Protein Levels in Blood Correlate with the Development of Cerebral Infarction Among Asians.

Mol Neurobiol 2016 May 2;53(4):2659-67. Epub 2015 Jul 2.

Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Our meta-analysis was designed to obtain the correlation between thrombomodulin (TM) and high-sensitive C-reactive protein (hs-CRP) levels and the development of cerebral infarction (CI). Case-control studies relevant to the role plasma TM levels and serum hs-CRP levels in the development of CI were retrieved both electronically and manually and further screened according to a predetermined inclusion and exclusion criteria. All enrolled studies were analyzed for meta-regression analyses, sensitivity analyses, and assessments of publication bias. Comprehensive Meta-analysis 2.0 software (CMA 2.0) was used for statistical analysis. A total of 359 studies were initially retrieved, and 13 studies were eventually recruited into our meta-analysis with a total of 881 CI patients (plasma TM levels: n = 513; serum hs-CRP levels: n = 368) and 1067 healthy controls. The results of our meta-analysis suggested that plasma TM levels and serum hs-CRP levels in CI patients were significantly higher than those in healthy controls. In conclusion, increased plasma TM levels and serum hs-CRP levels in CI patients were associated with the development of CI in Asians.
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http://dx.doi.org/10.1007/s12035-015-9279-yDOI Listing
May 2016

[Study on the relations among the screen-based sedentary behaviors, family factors and body mass index of children].

Beijing Da Xue Xue Bao Yi Xue Ban 2015 Jun;47(3):390-4

Department of Child, Adolescent and Women's Health, Institute of Child and Adolescent Health, Peking University School of Public Health, Beijing 100191, China.

Objective: To explore the relations among screen-based sedentary behaviors (SSB), family factors and body mass index (BMI) of children, and to study how family factors have effect on BMI through influencing SSB.

Methods: A total of 1,846 students aged 7-11 years from 12 primary schools in one district of Beijing were included. Their body weight and height were measured to calculate the BMI. The time of SSB and family factors were investigated by using questionnaires. The time of SSB was the total time of watching TV and videos, playing computer games and iPad each day during the past 7 days recalled by children. The family factors included the parents' education, occupation, the parents'time of SSB, whether the parents told their child the harm of SSB, the parents'time limit for the children's SSB. The parents'education and occupation were used for calculating the family socioeconomic score.

Results: The median time of SSB for children was 1 hour/day, and the interquartile range was 1 hour/day. The BMI of the children with the parents' time limit for the children's SSB less than 120 min/day were smaller than the children with the parents'time limit not less than 120 min/day, in both the boys (1.63 kg/m2, P<0.001) and the girls (0.85 kg/m2, P=0.004). The family socioeconomic score, the parents'SSB time, whether the parents told their children the harm of SSB were not related to the children's BMI . The mediation effects of SSB time for children on the association between the parents'time limit for the children's SSB and BMI were -0.222 kg/m2 (95%CI:-0.432, -0.095) for boys and -0.187 kg/m2 (95%CI: -0.507, -0.049) for girls, which accounted for 13.67% of the total effects for boys and 22.11% for girls.

Conclusion: The parents' time limit for the children's SSB has effect on their BMI through influencing their SSB time. Parents' supervision on the behaviors of children produces larger benefit for BMI than health education conveyed by parents. Therefore, parents' participation in supervising the behaviors of children are indispensable for preventing and controlling childhood obesity.
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June 2015

Influence of GNB3 C825T polymorphism on the efficacy of antidepressants in the treatment of major depressive disorder: A meta-analysis.

J Affect Disord 2015 Feb 2;172:103-9. Epub 2014 Oct 2.

Center for the Treatment and Study of Anxiety, University of Pennsylvania, Philadelphia, PA 19104, USA.

Objective: We performed the present meta-analysis in order to evaluate the influence of a common polymorphism (C825T, rs5443 C>T) in the GNB3 gene on the efficacy of antidepressants in the treatment of major depressive disorder (MDD).

Method: A relevant literature was searched using the PubMed, Embase, Web of Science, Cochrane Library, CISCOM, CINAHL, Google Scholar, CBM and CNKI databases without any language restrictions. STATA Version 12.0 software (Stata Corporation, College Station, Texas USA) was used for this meta-analysis. Odds ratio (OR) and its corresponding 95% confidence interval (95% CI) were calculated.

Results: Our findings suggested that the GNB3 C825T polymorphism was significantly correlated with a higher response rate to antidepressants in MDD patients under the allele and dominant models. Furthermore, we found significant associations between GNB3 C825T polymorphisms and antidepressant-induced remission in MDD patients. Ethnicity-stratified analysis indicated that GNB3 C825T polymorphisms may be strongly related to the efficacy of antidepressants in the treatment of MDD among Asians, but not in Caucasians (all P>0.05).

Conclusion: Our findings provide empirical evidence that GNB3 C825T polymorphisms may be correlated with the efficacy of antidepressants in the treatment of MDD, especially among Asians patients.
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http://dx.doi.org/10.1016/j.jad.2014.09.039DOI Listing
February 2015

Maintenance chemotherapy for ovarian cancer.

Cochrane Database Syst Rev 2013 Jun 29(6):CD007414. Epub 2013 Jun 29.

Department of Obstetrics and Gynecology, West China Second University Hospital, West China Women’s and Children’s Hospital,Chengdu, China.

Background: Epithelial ovarian cancer accounts for about 90% of all cases of ovarian cancer. Debulking surgery and six courses of platinum-based chemotherapy results in complete clinical remission (CCR) in up to 75% of cases. However, 75% of the responders will relapse within a median time of 18 to 28 months and only 20% to 40% of women will survive beyond five years. It has been suggested that maintenance chemotherapy could assist in prolonging remission. To date, there has not been a systematic review on the impact of maintenance chemotherapy for epithelial ovarian cancer.

Objectives: To assess the effectiveness and toxicity of maintenance chemotherapy for epithelial ovarian cancer and to evaluate the impact on quality of life (QoL).

Search Methods: In the original review we searched the Cochrane Gynaecological Cancer Review Group Specialised Register, The Cochrane Central Register of Controlled Trails (CENTRAL, The Cochrane Library 2009, Issue 1), MEDLINE, EMBASE, PubMed, CBMdisc, CNKI and VIP (to May 2009). We collected information from ongoing trials, checked reference lists of published articles and consulted experts in the field. For this update, the searches were extended to October 2012.

Selection Criteria: Randomised controlled trials (RCTs) comparing maintenance chemotherapy with no further intervention, maintenance radiotherapy or other maintenance therapy.

Data Collection And Analysis: Two review authors independently assessed trials for eligibility and quality and extracted data. We analysed overall survival (OS) and progression-free survival (PFS) rates as dichotomous variables. Toxicity and QoL data were extracted where present. All analyses were based on intention-to-treat (ITT) on the endpoint of survival. We also analysed data by subgroups of drugs.

Main Results: We included eight trials (1644 women). When all chemotherapy regimens were combined, meta-analysis indicated no significant difference in three-, five- and 10-year OS or PFS. For five-year OS, the combined risk ratio (RR) was 1.03 (95% confidence interval (CI) 0.96 to 1.10) and for the five-year PFS, the combined RR was 1.06 (95% CI 0.97 to 1.17). Results were very similar when trials of different regimens were analysed. Comparing chemotherapy with radiotherapy, only the RR for 10-year PFS in pathological complete remission (PCR) was in favour of whole abdominal radiotherapy 0.51 (95% CI 0.27 to 1.00), while three- and five-year OS rates have no significant difference between the two groups.

Authors' Conclusions: There is no evidence to suggest that the use of platinum agents, doxorubicin or paclitaxel used as maintenance chemotherapy is more effective than observation alone. Further investigations regarding the effect of paclitaxel used as maintenance chemotherapy are required.
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http://dx.doi.org/10.1002/14651858.CD007414.pub3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457821PMC
June 2013

Alcohol ablation at the posterior papillary muscle prevents ventricular fibrillation in swine without affecting mitral valve function.

Europace 2010 Dec 21;12(12):1781-6. Epub 2010 Sep 21.

Department of Cardiology, Chaoyang Hospital, Capital Medical University, Beijing 100020, People's Republic of China.

Aims: Radiofrequency ablation at the posterior papillary muscle (PM) significantly reduced ventricular fibrillation (VF) inducibility in rabbits and dogs, suggesting that PM may be involved in the generation of VF. However, the effect of ablation at the PM on VF inducibility remains unknown in normal intact swine hearts because in this species radiofrequency energy delivered at PM provoked incessant VF.

Methods And Results: Twelve anesthetized swine underwent median sternotomy. Under the ultrasonographic guidance, chemical ablation was performed via injection of dehydrated alcohol into the base of the posterior PM (group PM, n = 6) or anterior wall (control group, n = 6) in the left ventricle. Ventricular fibrillation inducibility and mitral valve function were measured pre- and post-ablation. Hearts were explanted and the ablated myocardium was stained with haematoxylin and eosin. Ventricular fibrillation inducibility was significantly decreased from 100 ± 0% pre-ablation to 11.9 ± 7.8% post-ablation in group PM (P = 0.001), whereas it was not statistically different in the control group (100 ± 0 vs. 92.9 ± 7.1%, pre-ablation vs. post-ablation). Haemorrhage and cellular necrosis was observed in the centre of ablated myocardium and no significant mitral regurgitation was observed following ablation at the posterior PM.

Conclusion: Alcohol ablation of the left posterior PM reduced VF inducibility in normal intact swine hearts, with no significant mitral regurgitation. This suggests that the posterior PM may be involved in the generation of VF, and the recurrence of VF may be prevented by chemical ablation at the posterior PM.
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http://dx.doi.org/10.1093/europace/euq337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988624PMC
December 2010

Maintenance chemotherapy for ovarian cancer.

Cochrane Database Syst Rev 2010 Sep 8(9):CD007414. Epub 2010 Sep 8.

Gynecology & Obstetrics Department, West China Second Hospital, Sichuan University, Clinical Medicine Dept of Sichuan University, Chengdu, Sichuan, China, 610041.

Background: Epithelial ovarian cancer accounts for about 90% of all cases of ovarian cancer. Debulking surgery and six courses of platinum-based chemotherapy results in complete clinical remission (CCR) in up to 75% of cases. However, 75% of the responders will relapse within a median time of 18 to 28 months and only 20% to 40% of women will survive beyond five years. It has been suggested that maintenance chemotherapy could assist in prolonging remission. To date, there has not been a systematic review on the impact of maintenance chemotherapy for epithelial ovarian cancer.

Objectives: To assess the effectiveness and toxicity of maintenance chemotherapy for epithelial ovarian cancer and to evaluate the impact on quality of life (QoL).

Search Strategy: We searched the Cochrane Gynaecological Cancer Review Group Specialized Register, The Cochrane Central Register of Controlled Trails (CENTRAL, The Cochrane Library Issue1, 2009), MEDLINE, EMBASE, PubMed, CBMdisc, CNKI and VIP (to May 2009). We collected information from ongoing trials, checked reference lists of published articles and consulted experts in the field.

Selection Criteria: Randomised controlled trials (RCTs) comparing maintenance chemotherapy with no further intervention, maintenance radiotherapy or other maintenance therapy.

Data Collection And Analysis: Two review authors independently assessed trials for eligibility and quality and extracted data. We analysed overall survival (OS) and progression free survival (PFS) rates as dichotomous variables. Toxicity and QoL data were extracted where present. All analyses were based on intention to treat (ITT) on the endpoint of survival. We also analysed data by subgroups of drugs.

Main Results: We included six trials(902 women). When all chemotherapy regimens were combined, meta-analysis indicated no significant difference in 3, 5 and 10-year OS or PFS. For 5-year OS, the combined relative risk (RR) was 1.07 (95% confidence interval (CI) 0.91 to 1.27) and for the 5-year PFS the combined RR was 1.18 (95% CI 0.88 to 1.58). Results were very similar when trials of different regimens were analysed. Comparing chemotherapy with radiotherapy, only the RR for 10-year PFS in pathological complete remission was in favour of whole abdominal radiotherapy 0.51 (95% CI 0.27 to 1.00), while 3 and 5-year OS rates have no significant difference between the two groups.

Authors' Conclusions: There is no evidence to suggest that the use of platinum agents or doxorubicin used as maintenance chemotherapy is more effective than observation alone. Further investigations regarding the effect of paclitaxel used as maintenance chemotherapy are required.
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http://dx.doi.org/10.1002/14651858.CD007414.pub2DOI Listing
September 2010

[Cytochemical observation on the developing anthers of Allium cepa L].

Fen Zi Xi Bao Sheng Wu Xue Bao 2007 Dec;40(6):451-7

School of Life Sciences, Xiamen University, Xiamen 361005.

Polysaccharides was detected using the periodic-acid-Schiff's (PAS) technique and lipid detected using Sudan black during the anther development of Allium cepa L. Before the meiosis of microspore mother cells there were a few lipid drops in endothecium cells and little polysaccharides in tapetal cells which did not differentiate completely in young anthers. At the stage of tetrad there were still few polysaccharides and lipid material in young anther, and only cell wall of anther wall and callose wall of tetrads displayed the feature of polysaccharids. The size of tapetal cells began to increase at this stage. During microspore development the tapetal cells reached its maximal size, and many lipid drops were accumulated in the cells. However, few lipid drops and starches appeared in microspores. At early stage of 2-celled pollen, the vegetative cell of 2-celled pollen began to accumulate starches. Tapetal cells degenerated at this stage and its lipid drops concentrated to form lipid block. Then the starches in 2-celled pollen disappeared with pollen development, and many lipid drops were accumulated in vegetative cell of nearly mature pollen.
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December 2007

[Calcium distribution in the egg cell, zygote and proembryo of lettuce (Lactuca sativa L.)].

Fen Zi Xi Bao Sheng Wu Xue Bao 2006 Feb;39(1):29-38

School of Life Sciences, Hunan Normal University, Changsha 410081.

Potassium antimonite precipitation was used to located calcium in the egg cells (before and after anthesis), zygotes and proembryos of lettuce (Lactuca sativa L.). A few calcium precipitates (ppts) were located in the small vacuoles of cytoplasm of egg cell at 3 d before anthesis, when egg cells just formed. Then the small vacuoles fused to form some bigger vacuoles in egg cell at 2d before anthesis. Calcium ppts increased evidently in the cytoplasm and nucleus of egg cells at this time. At 1d before anthesis, a biggest vacuole located at the micropyle end of the cell and its nucleus was pushed toward the chalazal end of the cell, which made an evident cellular polarity. The number of calcium ppts in the egg cell markedly decreased, suggesting that change of calcium distribution may be related to the development of egg cell. After anthesis and before fertilization, calcium ppts were still few in the egg cells, and most of them were accumulated in the nucleus, especially in the vacuoles of nucleolus. At 4h after anthesis, egg cell was fertilized and the wall at the chalazal end of egg cell was formed completely. Calcium ppts evidently increased again in egg cell, and some big ppts appeared in the karyoplasm of nucleus and abundant small ppts in the large vacuole. At 9h after anthesis, zygote completed its first division. Calcium ppts in the nucleus and cytoplasm of two-celled proembryo began to decrease, and only some ones accumulated in the vacuoles of nucleolus. At 18h after anthesis, zygote divided several times and became a multi-celled proembryo. Calcium ppts in the cells of proembryo ulteriorly diminished but there were many ppts on the surface of proembryo. The result indicates that calcium in egg cell, zygote and the cells of proembryo orderly changes its temporal and spatial position, which suggests that calcium may play a role during the development of egg cell and zygote.
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February 2006

Soluble multimer of recombinant endostatin expressed in E. coli has anti-angiogenesis activity.

Biochem Biophys Res Commun 2006 Jul 15;345(4):1398-404. Epub 2006 May 15.

Model Animal Research Center, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, PR China.

The bioactivity, refolding, and multimer formation of endostatin, particularly of recombinant endostatin produced from bacteria, are proved challenging for clinical application. In order to determine the biological activity of recombinant endostatin multimer, first, we expressed endostatin in Escherichia coli and purified it with ion-exchange chromatography. The purified active protein could elicit multimer formation spontaneously, but still has comparable activity. Aim to determine the anti-angiogenic activity of multimer endostatin, by use of RP-HPLC, we then successfully separated endostatin monomer and multimer for subjecting to anti-angiogenesis assay. The results from CAM (chorioallantoic membrane) inhibition assay showed that both monomer and multimer suppressed CAM vascularization significantly. At the dosage of 0.8 microg, inhibition rates of multimeric and monomeric proteins were about 58% and 38%, respectively. Multimeric endostatin exerted a higher activity than monomeric endostatin (p < 0.05). However, when the protein dosage is less than 0.4 microg/ml, there is no significance between their inhibition rates (p > 0.05), although both of them show a high inhibition effect in contrast to control. The results from HUVEC proliferation assay also showed similar effects at dosages of 0.6 and 1.6 microg/ml, multimer exerted a higher activity on inhibition of HUVEC proliferation comparing with monomer (p < 0.05). In conclusion, our results suggest that endostatin multimer has a comparable or higher bioactivity and multimerization will not affect its bioactivity, implying that endostatin activity is insensitive to structure conformation contributed by disulfide bonds.
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http://dx.doi.org/10.1016/j.bbrc.2006.05.031DOI Listing
July 2006

Microfilament-binding properties of N-terminal extension of the isoform of smooth muscle long myosin light chain kinase.

Cell Res 2006 Apr;16(4):367-76

Model Animal Research Center and National Key Lab of Medicine, Nanjing University, Nanjing 210061, China.

Myosin light chain kinases (MLCK) phosphorylate the regulatory light chain of myosin II in thick filaments and bind to F-actin-containing thin filaments with high affinity. The ability of short myosin light chain kinase (S-MLCK) to bind F-actin is structurally attributed to the DFRXXL regions in its N-terminus. The long myosin light chain kinase (L-MLCK) has two additional DFRXXL motifs and six Ig-like modules in its N-terminal extension. The six Ig-like modules are capable of binding to stress fibers independently. Our results from the imaging analysis demonstrated that the first two intact Ig-like modules (2Ig) in N-terminal extension of L-MLCK is the minimal binding module required for microfilament binding. Binding assay confirmed that F-actin was able to bind 2Ig. Stoichiometries of 2Ig peptide were similar for myofilament or pure F-actin. The binding affinities were slightly lower than 5DFRXXL peptide as reported previously. Similar to DFRXXL peptides, the 2Ig peptide also caused efficient F-actin bundle formation in vitro. In the living cell, over-expression of 2Ig fragment increased "spike"-like protrusion formation with over-bundled F-actin. Our results suggest that L-MLCK may act as a potent F-actin bundling protein via its DFRXXL region and the 2Ig region, implying that L-MLCK plays a role in cytoskeleton organization.
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http://dx.doi.org/10.1038/sj.cr.7310047DOI Listing
April 2006

[The distribution of ATPase in developmental anther of rice].

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2006 Feb;32(1):113-22

School of Life Sciences, Xiamen University, Xiamen 361005, China.

The distribution of ATPase was studied using lead precipitation technique during anther development in rice. The ATPase reactive precipitates (ppts) were located mostly in the nucleus of microspore mother cells (MMC) and only a few in the cytoplasm (Plate I-1). Anther wall had differentiated into four layers of cells and a few precipitates were located in the cells except the nucleus of tapetal cells where there were many ATPase reactive precipitates (Plate I-2). After meiosis of MMC, tapetal cells formed many endoplasmic reticula in its cytoplasm but still contained a few ppts. In the cells of epidermis, endothelium and middle layer, the ppts increased evidently in plasma membrane and near cytoplasm than before (Plate I-5). There were a large number of ppts located in the pollen wall during pollen development (Plate I-6), suggesting that ATPase is necessary for the construction of pollen wall. The exine of pollen wall of rice was constructed during microspore development and consisted of sporopollenin which came from tapetal cells. The ppts in exine also came from tapetal cell (Plate II-7). The intine of pollen wall was constructed during the stage of 2-cellular pollen and consisted of cellulose material coming from vegetative cell of pollen. The ATPase and ppts in intine came from vegetative cell of pollen (Plate III-7). Vegetative cell contained more ppts than generative cell during the development of 2-cellular pollen (Plate II-4, 5). The amount of ppts between two sperm cells in a pollen grain was also different (Plate IV-3,4). The physiological functions of ATPase located in different cells and different parts in the cells during anther development of rice were analyzed.
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February 2006

[Advances in cell biological researches on male sterility of higher plants].

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2006 Feb;32(1):17-23

College of Fisheries, Jimei University, Xiamen 361021, China.

Male sterility of higher plants is multiform in pollen abortion and has varied and complicated mechanisms. It is an active field to probe the mechanisms. Recently, some new results in this field have been obtained by using the methods of cell biology, including the structure and function of tapetal cell, the changes in Ca(2+) distribution, ATPase activity distribution, cytoskeleton array and programmed cell death in anther cells. All of the results gave us some new understanding for the process of pollen abortion. These results will make a link between the researches of individual and molecular level in male sterility of higher plants, and help us understand the mechanisms of male sterility of higher plants. This paper summarizes the knowledge about aborting process of male sterile anther obtained by the methods of cell biology.
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February 2006
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