Publications by authors named "Dong Zhang"

2,250 Publications

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Prognostic value and potential biological functions of ferroptosis-related gene signature in bladder cancer.

Oncol Lett 2022 Sep 5;24(3):301. Epub 2022 Jul 5.

Department of Urology, Suzhou Ninth People's Hospital, Soochow University, Suzhou, Jiangsu 215299, P.R. China.

Bladder cancer (BC), as a genitourinary system tumor, is a highly prevalent tumor type. Ferroptosis is an iron-dependent oxidative cell death mechanism that is becoming increasingly recognized as a promising avenue for cancer therapy. However, further determination of the prospective prognostic value of ferroptosis for BC and investigation of the underlying mechanisms is required. The mRNA expression profiles and associated clinical data were downloaded from public databases such as The Cancer Genome Atlas, Gene Expression Omnibus and the IMvigor210 database. To construct a predictive formula, the least absolute shrinkage and selection operator Cox regression algorithm was used. In addition, a prognostic multigene signature was constructed using previously selected ferroptosis-related genes (FRGs). A total of 28 FRGs were differentially expressed between tumor and normal samples with |log2 fold change| >1 and adjusted P<0.05. A prognostic model was then established and it was validated in the GEO cohort using six genes: Glutamate-cysteine ligase modifier subunit, crystallin α-B, transferrin receptor, zinc finger E-box binding homeobox 1, squalene epoxidase and glucose-6-phosphate dehydrogenase (G6PD). Numerous important pathways involved in the development of the immune system and cancer were indicated to be significantly different between the two risk groups. In addition, it was discovered that G6PD expression subgroups that were associated with immunotherapy response in patients with BC had similar prognostic features to risk score subgroups. In the present study, a gene signature with a prognostic value for ferroptosis in BC was successfully developed and the potential value of G6PD was identified for future research.
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http://dx.doi.org/10.3892/ol.2022.13421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353228PMC
September 2022

Comparative transcriptome and metabolome analyses identified the mode of sucrose degradation as a metabolic marker for early vegetative propagation in bulbs of Lycoris.

Plant J 2022 Aug 9. Epub 2022 Aug 9.

Genomics and Genetic Engineering Laboratory of Ornamental Plants, Zhejiang University, Hangzhou, 310058, China.

Vegetative propagation (VP) is an important practice for production in many horticultural plants. Sugar supply constitutes the basis of VP in bulb flowers, but the underlying molecular basis remains elusive. By performing a combined sequencing technologies coupled with UHPLC-Q-TOF MS approach for metabolic analyses, we compared two Lycoris species with contrasting regeneration rates, high-regeneration L. sprengeri (Ls) and low-regeneration L. aurea (La). A comprehensive multi-omics analyses identified both expected processes involving carbohydrate metabolism and transcription factor networks and novel metabolic characteristics for each developmental stage. Higher abundance of the differentially expressed genes (DEGs) including those encoding ethylene responsive factors was detected at bulblet initiation stage as compared to the late stage of bulblet development. High hexose-to-sucrose ratio correlated to bulblet formation across all the species examined, indicating its role in VP process in Lycoris bulb. Importantly, a clear difference between cell wall invertase (CWIN)-catalyzed sucrose unloading in high-regeneration species and the sucrose synthase (SUS)-catalyzed pathway in low-regeneration species was observed at the bulblet initiation stage, which was supported by findings from carboxyfluorescein tracing and quantitative real-time PCR analyses. Collectively, the findings indicate a sugar-mediated model on the regulation of VP in which high CWIN expression or activity may promote bulblet initiation via enhancing apoplasmic unloading of sucrose or sugar signals, while the subsequent high ratio of hexose-to-sucrose likely supports cell division characterized in the next phase of bulblet formation.
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http://dx.doi.org/10.1111/tpj.15935DOI Listing
August 2022

A panel of lipid markers for rice discrimination of Wuchang Daohuaxiang in China.

Food Res Int 2022 Sep 14;159:111511. Epub 2022 Jun 14.

Academy of National Food and Strategic Reserves Administration, Beijing 100037, PR China.

Wuchang Daohuaxiang (DHX) rice is a famous product in China, while identification and authentication method is limited. Due to the special taste and flavor of DHX, lipidomics was conducted to discriminate it from faked rice. First, lipid profiles of DHX and other nine rice varieties were required from the established UPLC-Q/TOF-MS/MS method. The compositions and contents of lipids significantly varied among different rice. Then, twenty-six potential biomarkers containing 6 DGs, 5 PAs, 4 PCs, 1 PE, 3 PGs, 2 PIs, 2 PSs, 2 SMs, and 1 Cer were characterized and verified for identifying DHX from all faked rice. The accuracy of developed methods for DHX discrimination was 97.14%. In addition, OPLS-DA models were established for DHX distinction between each of the nine rice cultivars. Our results would supply as supplementary tools for discrimination of DHX rice for supervision departments and industries.
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http://dx.doi.org/10.1016/j.foodres.2022.111511DOI Listing
September 2022

Efficacy, safety and immunogenicity of hexavalent rotavirus vaccine in Chinese infants.

Virol Sin 2022 Aug 1. Epub 2022 Aug 1.

National Engineering Technology Research Center for Combined Vaccines, Wuhan Institute of Biological Product Co., Ltd., Wuhan, 430207, China.

A randomized, double-blind, placebo-controlled multicenter trial was conducted in healthy Chinese infants to assess the efficacy and safety of a hexavalent live human-bovine reassortant rotavirus vaccine (HRV) against rotavirus gastroenteritis (RVGE). A total of 6400 participants aged 6-12 weeks were enrolled and randomly assigned to either HRV (n = 3200) or placebo (n = 3200) group. All the subjects received three oral doses of vaccine four weeks apart. The vaccine efficacy (VE) against RVGE caused by rotavirus serotypes contained in HRV was evaluated from 14 days after three doses of administration up until the end of the second rotavirus season. VE against severe RVGE, VE against RVGE hospitalization caused by serotypes contained in HRV, and VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were also investigated. All adverse events (AEs) were collected for 30 days after each dose. Serious AEs (SAEs) and intussusception cases were collected during the entire study. Our data showed that VE against RVGE caused by serotypes contained in HRV was 69.21% (95%CI: 53.31-79.69). VE against severe RVGE and VE against RVGE hospitalization caused by serotypes contained in HRV were 91.36% (95%CI: 78.45-96.53) and 89.21% (95%CI: 64.51-96.72) respectively. VE against RVGE, severe RVGE, and RVGE hospitalization caused by naturally infecting any serotype of rotavirus were 62.88% (95%CI: 49.11-72.92), 85.51% (95%CI: 72.74-92.30) and 83.68% (95%CI: 61.34-93.11). Incidences of AEs from the first dose to one month post the third dose in HRV and placebo groups were comparable. There was no significant difference in incidences of SAEs in HRV and placebo groups. This study shows that this hexavalent reassortant rotavirus vaccine is an effective, well-tolerated, and safe vaccine for Chinese infants.
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http://dx.doi.org/10.1016/j.virs.2022.07.011DOI Listing
August 2022

The Relation between Immunological Features and the Positive SARS-CoV-2 Nucleic Acid in Patients with Nonsevere COVID⁃19.

Can J Infect Dis Med Microbiol 2022 30;2022:4270096. Epub 2022 Jul 30.

Tianjin Institute of Respiratory Diseases, Tianjin 300350, China.

Objective: The novel coronavirus nucleic acid results are the core indicators of illness monitoring. This study aimed to evaluate the relationship between immunological features and positive SARS-CoV-2 nucleic acid by analyzing the clinical and immunological features in nonsevere COVID-19 cases.

Methods: Data from nonsevere COVID-19 patients admitted to Haihe Hospital from May 2020 to June 2021 were retrospectively reviewed and analyzed.

Results: (1) A total of 122 cases were reviewed in the present study, including 38 mild and 84 moderate cases. The average age of mild cases was significantly different from moderate cases ( < 0.001). Eight patients complained of hyposmia and it was more frequent in mild cases ( < 0.001). The nucleic acid positive duration (NPD) of nonsevere novel coronavirus was 20.49 (confidence interval (CI) 17.50-3.49) days. (2) The levels of specific IgM and IgG for COVID-19 were higher in mild cases than in moderate cases (=0.023 and =0.047, respectively). (3) The correlation analysis with antibodies and T-cell subtypes showed that the lymphocyte (LYM) count, T cells, CD4T cells, and CD8T cells had a linear correlation with NPD. (4) Among the 93 patients monitored, 62 COVID-19 cases presented a progressive rise of specific IgM and IgG. The daily increase rates of IgM and IgG were 38.42% (CI 28.22-48.61%) and 24.90% (CI 0.23-29.58%), respectively.

Conclusion: The levels and daily increase rates of specific IgM and IgG against the virus can vary between cases. The NPD presented a linear correlation with the LYM, T cells, CD4T cells, and CD8+T cells. Hence, more attention should be paid to these indicators in clinical practice.
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http://dx.doi.org/10.1155/2022/4270096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338729PMC
July 2022

Unexpected steric hindrance failure in the gas phase F + (CH)CI S2 reaction.

Nat Commun 2022 Jul 30;13(1):4427. Epub 2022 Jul 30.

State Key Laboratory of Molecular Reaction Dynamics and Center for Theoretical and Computational Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian, 116023, China.

Base-induced elimination (E2) and bimolecular nucleophilic substitution (S2) reactions are of significant importance in physical organic chemistry. The textbook example of the retardation of S2 reactivity by bulky alkyl substitution is widely accepted based on the static analysis of molecular structure and steric environment. However, the direct dynamical evidence of the steric hindrance of S2 from experiment or theory remains rare. Here, we report an unprecedented full-dimensional (39-dimensional) machine learning-based potential energy surface for the 15-atom F + (CH)CI reaction, facilitating the reliable and efficient reaction dynamics simulations that can reproduce well the experimental outcomes and examine associated atomic-molecular level mechanisms. Moreover, we found surprisingly high "intrinsic" reactivity of S2 when the E2 pathway is completely blocked, indicating the reaction that intends to proceed via E2 transits to S2 instead, due to a shared pre-reaction minimum. This finding indicates that the competing factor of E2 but not the steric hindrance determines the small reactivity of S2 for the F + (CH)CI reaction. Our study provides new insight into the dynamical origin that determines the intrinsic reactivity in gas-phase organic chemistry.
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http://dx.doi.org/10.1038/s41467-022-32191-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338938PMC
July 2022

Sequencing and phylogenomics of the complete mitochondrial genome of Allodiplogaster sp. (Rhabditida: Diplogasteridae): A new gene order and its phylogenetic implications.

Gene 2022 Oct 26;840:146761. Epub 2022 Jul 26.

State Key Laboratory of Grassland Agro-Ecosystems, and College of Ecology, Lanzhou University, Lanzhou 730000, China. Electronic address:

Gene order has been utilized as a phylogenetic signal for many taxa. However, its phylogenetic performance has not been evaluated in Nematoda. As there is only one nematode mitogenome available to date, in the Diplogasteridae family, we sequenced the mitogenome of Allodiplogaster sp. and constructed a phylogeny for Nematoda using this updated mitogenome dataset. We then compared this phylogeny to one constructed using gene order. The complete mitochondrial genome of Allodiplogaster sp. was 13,953 bp in size and included 22 tRNAs, two rRNAs, and 12 protein-coding genes. To assess how Allodiplogaster sp. is related to other nematode species, we used Bayesian inference and maximum likelihood algorithms to construct phylogenetic trees of the Nematoda. We found that: 1) The target species Allodiplogaster sp. is closely related to Allodiplogaster sudhausi. The topology of the mitogenome based phylogeny was nearly identical to previous phylogenies created using 18S rRNA data, except for the placement of the Strongyloididae family. 2) The maximum likelihood tree constructed using gene order was roughly consistent with the mitogenome-based tree at the family level, but not at the species level. 3) Protein-coding genes were ordered differently in Allodiplogaster sp. versus Allodiplogaster sudhausi; this represents the first report of such a reordering in the class Chromadorea in our study. Our study confirms that gene order represents useful phylogenetic information for the Nematoda: the maximum likelihood tree based on gene order provided additional support for the nematode phylogeny constructed using molecular data.
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http://dx.doi.org/10.1016/j.gene.2022.146761DOI Listing
October 2022

Association between obstructive sleep apnea (OSA) and atrial fibrillation (AF): A dose-response meta-analysis.

Medicine (Baltimore) 2022 Jul 29;101(30):e29443. Epub 2022 Jul 29.

Department of Cardiology, Xijing Hospital, The Air Force Military Medical University, Xi'an Shaanxi, China.Supplemental Digital Content is available for this article.

Background: Refractory hypoxemia episodes are characteristic of obstructive sleep apnea (OSA). Patients with OSA suffer from oxidative stress in all systems. Atrial fibrillation (AF) is a type of arrhythmia that may be induced by OSA. In this study, we explored the dose-response relationship between OSA and AF. Our research provides the basis for a novel approach to AF prevention.

Methods: We screened four databases (PubMed, Embase, the Cochrane Library, and Web of Science) for observational studies on OSA and AF. Studies were collected from database establishment to November 2020. We performed a traditional subgroup meta-analysis. Linear and spline dose-response models were applied to assess the association between the apnea-hypopnea index, an indicator of OSA severity, and the risk of AF. Review Manager version 5.3 software and Stata 16.0 were used for the analysis.

Results: Sixteen observational studies were included in the study. We excluded a study from the conventional meta-analysis. In the subgroup analysis, the odds ratios for new onset AF for no obvious reason, new onset AF after surgical operations, such as coronary artery bypass grafting, and AF after ablation treatment were 1.71 (95% CI 1.37-2.13, P < .05), 2.65 (95% CI 2.32-3.01, P < .05), and 2.93 (95% CI 2.47-3.49, P < .05), respectively. Linear dose-response meta-analysis results revealed that the risk of AF increased with increasing apnea-hypopnea index value.

Conclusion: Through dose-response meta-analysis, we found a potential dose-response relationship between OSA severity and the risk of AF. This relationship should be considered in interventions aimed at AF prevention in the future.
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http://dx.doi.org/10.1097/MD.0000000000029443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333485PMC
July 2022

Insights into the complicated networks contribute to adventitious rooting in transgenic MdWOX11 apple microshoots under nitrate treatments.

Plant Cell Environ 2022 Jul 28. Epub 2022 Jul 28.

College of Horticulture, Yangling Sub-Center of the National Center for Apple Improvement, Northwest A & F University, Yangling, 712100, Shaanxi, P. R. China.

Adventitious root formation is a bottleneck for the mass propagation of microshoots, and nitrate is an essential nutrient regulating adventitious roots. WOX11 is involved in adventitious rooting. But the crosstalk between nitrate and WOX11 is completely unknown. In this study, MdWOX11 transgenic apple microshoots were grown on different nitrate treatments. Low nitrate promotes adventitious rooting in overexpressed microshoots more than wild type and RNA interference microshoots. In contrast, medium nitrate significantly inhibits it in overexpressed and RNA interference microshoots compared with wild type microshoots. Stem anatomy indicated that medium nitrate delays root primordia formation compared with low nitrate. Methyl jasmonate and zeatin riboside played positive and negative roles in adventitious rooting, respectively. Transcriptomic analysis was conducted to understand the molecular mechanisms behind the phenotypes better. Hormone signaling, sugar metabolism, nitrogen metabolism, cell cycle, and root development pathway-related genes were selected for their potential involvement in adventitious rooting. Results suggest that methyl jasmonate signals act as positive regulators of specific invertases, increasing sink strength in stem basal parts and promoting adventitious rooting in low nitrate-treated microshoots. Moreover, nitrogen signaling and MdWOX11 expression affect cytokinin accumulation and response to cytokinin through regulating the expression of genes related to cytokinin synthesis and transduction pathways, which ultimately affect adventitious rooting. This study provided important insights into the complicated networks involved in adventitious rooting in transgenic microshoots under nitrate treatments. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/pce.14409DOI Listing
July 2022

Dehydrative Cross-Coupling for C-N Bond Construction under Transition-Metal-Free Conditions.

Org Lett 2022 Aug 28;24(31):5657-5662. Epub 2022 Jul 28.

School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, P.R. China.

A transition-metal-free catalytic system was designed to address the dehydrative cross-coupling of unactivated primary/secondary alcohols with amines/amides under environmentally benign conditions. Mg and counteranion (PF) worked synergistically to realize C-OH bond cleavage and concomitant C-N bond formation. A wide range of allylic alcohols and amines/amides were tolerated well in this transformation, which allowed C-N bond construction with high efficiency.
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http://dx.doi.org/10.1021/acs.orglett.2c01902DOI Listing
August 2022

Effect of Silver Nanoparticles With Thermoplastic Polyurethane on Postoperative Rehabilitation of Diabetic Patients With Open Fracture of Lower Extremities.

Front Surg 2022 11;9:954155. Epub 2022 Jul 11.

Department of Traumatology and Orthopaedics, Beijing Jishuitan Hospital (The Fourth Medical College of Peking University), Beijing, China.

Objective: This retrospective study aims to explore the effect of silver nanoparticles with thermoplastic polyurethane (TPU/NS) on the rehabilitation of diabetic patients with open fracture of lower extremities.

Methods: Diabetic patients ( = 98) with open fracture of lower extremities treated in our hospital were analyzed retrospectively from June 2015 to December 2021. TPU/NS nanocomposites were prepared for postoperative treatment of diabetic patients with open fracture of lower extremities. First, the cultured and were used to test the antibacterial effect of TPU/NS dressing . After using TPU/NS dressing (observation group) and traditional dressing (control group), the inflammatory reaction, clinical treatment, functional rehabilitation, and adverse reactions in patients were compared.

Results: TPU/NS dressing effectively inhibited the growth of bacteria with a minimum inhibitory concentration of 2 μg/mL. The usage of TPU/NS dressing reduced the inflammatory reaction by reducing positive rate of bacteria after the dressing on the seventh day postoperatively. Besides, the times of dressing, stopping time of wound exudation, wound healing time, length of hospital stay, and VAS score in the observation group were lower than those in the control group; the incidence of adverse reactions after treatment was lower in the observation group as compared with the control group (17.07% vs. 35.09%). Meanwhile, the functional rehabilitation and life quality of patients in the observation group were better TPU/NS dressing treatment.

Conclusion: TPU/NS dressing has the function of promoting the postoperative recovery of patients by inhibiting the bacterial infection of the wound, thus improving the limb function and life quality. As a result, there was a tremendous potential to apply the constructed TPU/NS membrane to diabetic patients with open fractures, especially those with soft tissue injury.
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http://dx.doi.org/10.3389/fsurg.2022.954155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309470PMC
July 2022

Influence of prazosin on systemic iron levels and the associated iron metabolic alterations in spontaneously hypertensive rats.

Pharmacol Res Perspect 2022 Aug;10(4):e00991

College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, People's Republic of China.

The relationship between cardiovascular diseases and iron disorders has gained increasing attention; however, the effects of hypotensive drugs on iron metabolic alterations in hypertension are not well understood. The purpose of this study was to investigate iron metabolic changes after prazosin treatment of spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats. Our second objective was to examine the effects of hypertension and anti-hypertensive drugs on bone formation and resorption. SHRs and WKY rats were randomized into either prazosin-treated groups (WKY + PZ and SHR + PZ) or untreated groups (WKY and SHR). After 7 days of intragastric prazosin administration, the rats were sacrificed for analysis; blood samples and organs (the duodenum, liver, kidneys, spleen, and femur) were collected. Both WKY + PZ and SHR groups exhibited iron deficiency in the serum and liver. Prazosin increased the iron levels in the bone tissue of SHRs. Prazosin stimulated the expression of hepcidin mRNA in the liver of SHRs and inhibited the expression of this iron-regulatory hormone in WKY rats. FPN1 expression in the duodenum was increased significantly in SHRs, however markedly decreased after prazosin treatment. The expression of TLR4 and Ctsk was enhanced in the bone tissue of SHRs, whereas CLC-7 expression was inhibited. Both hypotension and hypertension can lead to iron deficiency. Treatment with prazosin restored iron homeostasis in SHRs. The inverse impacts of prazosin on hepatic hepcidin expression in SHRs versus WKY rats indicates differing iron regulatory mechanisms between hypertensive and normal animals. The osteoclast activity was found to be enhanced in SHRs. Further study is needed to address whether the changes in osteoblast and osteoclast activity in SHRs correlates with the effects on iron metabolism.
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http://dx.doi.org/10.1002/prp2.991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326454PMC
August 2022

De-Implementation of Detrimental Feeding Practices in Childcare: Mixed Methods Evaluation of Community Partner Selected Strategies.

Nutrients 2022 Jul 12;14(14). Epub 2022 Jul 12.

Department of Pharmacy Practice and Psychiatry, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

This pilot evaluated strategies to decrease detrimental feeding practices in early care and education, which are hypothesized to compete with evidence-based feeding and obesity prevention practices. This study made two key comparisons: (1) a between-site comparison of sites receiving (a) no implementation or de-implementation strategies (i.e., Basic Support; B), (b) implementation strategies only (i.e., Enhanced Support; E), and (c) implementation and de-implementation strategies (i.e., De-implementation + Enhanced Support; D + E) and (2) a within-site pre-post comparison among sites with D + E. At nutrition lessons, the D + E group had more Positive Comments (Hedege's = 0.60) and higher Role Model fidelity (Hedege's = 1.34) compared to the E group. At meals, assistant teachers in the D + E group had higher Positive Comments than in the B group ( = 0.72). For within-group comparisons, the D + E group decreased Negative Comments ((19) = 2.842, = 0.01), increased Positive Comments ((20) = 2.314, = 0.031), and improved use of the program mascot at nutrition lessons ((21) = 3.899, = 0.001). At meals, lead teachers' Negative Comments decreased ((22) = 2.73, = 0.01). Qualitative data identified strengths and opportunities for iteration. Despite a COVID interruption, mid-point comparisons and qualitative feedback suggest promise of the de-implementation strategy package.
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http://dx.doi.org/10.3390/nu14142861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319894PMC
July 2022

Ultrastructure of Antennal Sensory Organs in Nine Flesh Flies (Diptera: Sarcophagidae): New Insight into the Definition of Family Sarcophagidae.

Insects 2022 Jun 30;13(7). Epub 2022 Jun 30.

School of Ecology and Nature Conservation, Beijing Forestry University, Qinghua East Road 35, Beijing 100083, China.

The antennae are the main olfactory organ of flies, playing key roles in their survival and the success of all life stages. Antennal ultrastructural morphology has been well described in the representative species of most calyptrate families, yet only a few studies have focused on Sarcophagidae species, those with ecological and medical relevance. Antennal morphology and the types, shapes, distribution, and density of the antennal sensilla of nine Sarcophagidae species are studied in detail with scanning electron microscopy, including Miltogramminae: (Fallén) and (Loew), Paramacronychiinae: (Rohdendorf & Verves), (Macquart), and . (Schiner); Sarcophaginae: () Meigen, . () (Wiedemann), . () (Robineau-Desvoidy), and . () (Rohdendorf), covering all three subfamilies of this family. The morphology of the three segments of the antennae has been described. The scape has only one type of chaetic sensilla, while three subtypes of chaetic sensilla were detected on the pedicel. The postpedicel has four types of sensilla: trichoid sensilla, coeloconic sensilla, clavate sensilla, and three subtypes of basiconic sensilla. Bottle-shaped sensilla were observed in sensory pits on the postpedicel in all nine species. These sensilla have not been discovered in other calyptrate species, suggesting that they are a potential sarcophagid synapomorphy.
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http://dx.doi.org/10.3390/insects13070602DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316071PMC
June 2022

mTOR-FABP4 signal is activated in brain arteriovenous malformations in humans.

J Mol Med (Berl) 2022 Jul 25. Epub 2022 Jul 25.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Arteriovenous malformations (AVMs) are the most common types of cerebral vascular malformations, which are dynamic lesions with de novo growth potentials. The dysfunction of endothelial cells has been postulated to play a role in the pathogenesis of brain AVMs. mTOR-FABP4 signal enhances the angiogenic responses of endothelial cells and is not activated in the normal cerebral vasculature. Herein, we investigated the hypothesis that the mTOR-FABP4 signal may be activated in brain AVMs. The abundance of molecules in mTOR-FABP4 signal expression was detected by immunohistochemistry and Western blotting; special expressing cells were further characterized by double immunofluorescence using antibodies against various cell-specific markers. Next, several functional assays were performed to analyze the influence of the mTOR-FABP4 signal on proliferation, apoptosis, migration, and vascular tube formation of endothelial cells in human umbilical vein endothelial cells (HUVECs) using rapamycin and L-leucine. The expression of mTOR, p-mTOR, and FABP4 was increased in endothelial cells of human brain AVMs. Endothelial cell mTOR and p-mTOR expression were present in 70% and 55% of brain AVMs, respectively. Moreover, a population of FABP4-positive endothelial cells was detected in 80% of brain AVMs. The mTOR-FABP4 signal was activated and inhibited by L-leucine and rapamycin in HUVECs. The proliferation, apoptosis, migration, and vascular tube formation of endothelial cells could be inhibited by rapamycin. The mTOR-FABP4 signal was activated in human brain AVMs, and the mTOR-FABP4 signal was involved in proliferation, apoptosis, migration, and the vascular tube formation of endothelial cells. Taken together, whether rapamycin has therapeutic potential for treating human brain AVMs is worthy of further study. KEY MESSAGES : We confirmed that the mTOR- FABP4 pathway is activated in human brain arteriovenous malformations. We confirmed that mTOR signaling pathway affects endothelial cell function by regulating proliferation, migration, apoptosis, and tube formation of endothelial cell. Our study can provide theoretical support for mTOR pathway inhibitors in the treatment of human brain arteriovenous malformations.
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http://dx.doi.org/10.1007/s00109-022-02237-9DOI Listing
July 2022

Granular Parakeratosis: A Case Report.

Clin Cosmet Investig Dermatol 2022 15;15:1367-1370. Epub 2022 Jul 15.

Department of Dermatology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, People's Republic of China.

Granular parakeratosis is an uncommon acquired keratinization disorder that occurs in the armpit, groin, and other parts of the body. It may be related to stimulation by detergents and antiperspirants. This article reports a case of granular parakeratosis in the groin. The patient was a young man with no predisposing factors. The clinical manifestations included symmetrical bilateral inguinal erythema, dryness, and a small amount of bran-like desquamation. After histopathological examination, the final diagnosis was granular parakeratosis, which was cured by topical application of glucocorticoid cream and silicone oil cream. Granular parakeratosis is a rare skin disease of unknown etiology. Clinicians need to pay attention to this disease and differentiate it from various diseases to avoid misdiagnosis.
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http://dx.doi.org/10.2147/CCID.S371558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297043PMC
July 2022

A ground-based remote sensing system for high-frequency and real-time monitoring of phytoplankton blooms.

J Hazard Mater 2022 Jul 16;439:129623. Epub 2022 Jul 16.

Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; Nanjing Zhongke Deep Insight Technology Research Institute Co., Ltd, Nanjing 211899, China.

The worldwide expansion of phytoplankton blooms has severely threatened water quality, food webs, habitat stability and human health. Due to the rapidity of phytoplankton migration and reproduction, high-frequency information on phytoplankton bloom dynamics is crucial for their forecasting, treatment, and management. While several approaches involving satellites, in situ observations and automated underwater monitoring stations have been widely used in the past several decades, they cannot fully provide high-frequency and continuous observations of phytoplankton blooms at low cost and with high accuracy. Thus, we propose a novel ground-based remote sensing system (GRSS) that can monitor real-time chlorophyll a concentrations (Chla) in inland waters with a high frequency. The GRSS mainly consists of three platforms: the spectral measurement platform, the data-processing platform, and the remote access control, display and storage platform. The GRSS is capable of obtaining a remote sensing irradiance ratio (R(λ)) of 400-1000 nm at a high frequency of 20 s. Eight different Chla retrieval algorithms were calibrated and validated using a dataset of 481 pairs of GRSS R(λ) and in situ Chla measurements collected from four inland waters. The results showed that random forest regression achieved the best performance in deriving Chla (R = 0.95, root mean square error = 13.40 μg/L, and mean relative error = 25.7%). The GRSS successfully captured two typical phytoplankton bloom events in August 2021 with rapid changes in Chla from 20 μg/L to 325 μg/L at the minute level, highlighting the critical role that this GRSS can play in the high-frequency monitoring of phytoplankton blooms. Although the algorithm embedded into the GRSS may be limited by the size of the training dataset, the high-frequency, continuous and real-time data acquisition capabilities of the GRSS can effectively compensate for the limitations of traditional observations. The initial application demonstrated that the GRSS can capture rapid changes of phytoplankton blooms in a short time and thus will play a critical role in phytoplankton bloom management. From a broader perspective, this approach can be extended to other carriers, such as aircraft, ships and unmanned aerial vehicles, to achieve the networked monitoring of phytoplankton blooms.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129623DOI Listing
July 2022

Amygdala abnormalities across disease stages in patients with sporadic amyotrophic lateral sclerosis.

Hum Brain Mapp 2022 Jul 22. Epub 2022 Jul 22.

Department of Neurology, Research Institute of Neuromuscular and Neurodegenerative Disease, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

To examine selective atrophy patterns and resting-state functional connectivity (FC) alterations in the amygdala at different stages of amyotrophic lateral sclerosis (ALS), and to explore any correlations between amygdala abnormalities and neuropsychiatric symptoms. We used the King's clinical staging system for ALS to divide 83 consecutive patients with ALS into comparable subgroups at different disease stages. We explored the pattern of selective amygdala subnucleus atrophy and amygdala-based whole-brain FC alteration in these patients and 94 healthy controls (HCs). Cognitive and emotional functions were also evaluated using a neuropsychological test battery. There were no significant differences between ALS patients at King's stage 1 and HCs for any amygdala subnucleus volumes. Compared with HCs, ALS patients at King's stage 2 had significantly lower left accessory basal nucleus and cortico-amygdaloid transition volumes. Furthermore, ALS patients at King's stage 3 demonstrated significant reductions in most amygdala subnucleus volumes and global amygdala volumes compared with HCs. Notably, amygdala-cuneus FC was increased in ALS patients at King's stage 3. Specific subnucleus volumes were significantly associated with Mini-Mental State Examination scores and Hamilton Anxiety Rating Scale scores in ALS patients. In conclusions, our study provides a comprehensive profile of amygdala abnormalities in ALS patients. The pattern of amygdala abnormalities in ALS patients differed greatly across King's clinical disease stages, and amygdala abnormalities are an important feature of patients with ALS at relatively advanced stages. Moreover, our findings suggest that amygdala volume may play an important role in anxiety and cognitive dysfunction in ALS patients.
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http://dx.doi.org/10.1002/hbm.26016DOI Listing
July 2022

Strong non-Arrhenius behavior at low temperatures in the OH + HCl → HO + Cl reaction due to resonance induced quantum tunneling.

Chem Sci 2022 Jul 13;13(26):7955-7961. Epub 2022 Jun 13.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian 116023 Liaoning China

The OH + HCl → HO + Cl reaction releases Cl atoms, which can catalyze the ozone destruction reaction in the stratosphere. The measured rate coefficients for the reaction deviate substantially from the Arrhenius limit at low temperatures and become essentially independent of temperature when < 250 K, apparently due to quantum tunneling; however, the nature of the quantum tunneling is unknown. Here, we report a time-dependent wave packet study of the reactions on two newly constructed potential energy surfaces. It is found that the OH + HCl reaction possesses many Feshbach resonances trapped in a bending/torsion excited vibrational adiabatic potential well in the entrance channel due to hydrogen bond interaction. These resonance states greatly induce quantum tunneling of a hydrogen atom through the reaction barrier, causing the reaction rates to deviate substantially from Arrhenius behavior at low temperature, as observed experimentally.
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http://dx.doi.org/10.1039/d2sc01958bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258319PMC
July 2022

MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple.

Front Plant Sci 2022 5;13:903808. Epub 2022 Jul 5.

College of Horticulture, Northwest A&F University, Yangling, China.

Flowering-related problems in "Fuji" apple have severely restricted the development of China's apple industry. Nuclear pore complexes (NPCs) control nucleoplasmic transport and play an important role in the regulation of plant growth and development. However, the effects of NPCs on apple flowering have not been reported. Here, we analysed the expression and function of , a component of apple NPC. expression was the highest in flower buds and maintained during 30-70 days after flowering. -overexpressing (OE) lines displayed significantly earlier flowering than that of the wild type. We further confirmed that MdNup54 interacts with MdHSP70, MdMYB11, and MdKNAT4/6. Consistent with these observations, flowering time of -OE lines was also significantly earlier. Therefore, our findings suggest a possible interaction of MdNup54 with MdHSP70 to mediate its nuclear and cytoplasmic transport and to regulate apple flowering. The results enhance the understanding of the flowering mechanism in apple and propose a novel strategy to study nucleoporins.
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http://dx.doi.org/10.3389/fpls.2022.903808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296068PMC
July 2022

Occupational socialization in pre-service physical education teachers.

Authors:
Dong Zhang

PLoS One 2022 21;17(7):e0271772. Epub 2022 Jul 21.

School of Physical Education, Soochow University, Suzhou, Jiangsu, China.

The purpose of this study was to examine differences in occupational socialization (OS) among pre-service physical education teachers' (PPETs) in four year groups. 238 PPETs from a Chinese university. An online survey and the semi-structured interviews were used to collect data. The younger PPETs felt more prepared and believed in the benefit of professional development more than the older PPETs. The PPETs' acculturation had a critical influence on their OS. The lack of early field experience may explain why the seniors felt less confident than their younger counterparts. This study would contribute a new angle to discuss the OS and physical education teacher education program quality, especially the design of the teaching practice opportunities, together.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0271772PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302786PMC
July 2022

Optimized acoustic streaming generated at oblique incident angles to improve ultrasound thrombolysis effect.

Med Phys 2022 Jul 21. Epub 2022 Jul 21.

Key Laboratory of Modern Acoustics (MOE), Department of Physics, Collaborative Innovation Center of Advanced Microstructure, Nanjing University, Nanjing, China.

Background: Combined with thrombolytic drugs and/or microbubbles, ultrasound (US) has been regarded as a useful tool for thrombolysis treatment by taking its advantages of noninvasive, non-ionization, low cost, and accurate targeting of tissues deep in body. Recently, low-intensity pulsed US, which can cause fewer complications by stable cavitation and acoustic streaming other than more violent effects, has attracted broad attention.

Purpose: However, the thrombolysis effect in practice might not achieve expectation because there is not an ideal parallel multilayered structure between the skin and the targeted vessel. Therefore, the current work aims to better elucidate the influence of US incident angle on the generation of acoustic streaming and thrombolysis effect.

Methods: Systemic numerical and experimental studies, namely, finite element modeling (FEM), particle image velocimetry (PIV), and in vitro thrombolysis measurements, were performed to estimate the acoustical/streaming field pattern, maximum flow velocity, and shear stress on the surface of thrombus, as well as the lysis rate generated at different conditions. These methods aim at verifying the hypothesis that streaming-induced vortices can further accelerate the dissolution of the thrombus and optimized thrombolysis effected can be achieved by adjusting US incident angles.

Results: The pool data results showed that the variation trends of the flow velocity and shear stress obtained from FEM simulation and PIV experiments are qualitatively consistent with each other. There exists an optimal incident angle that can maximize the flow velocity and shear stress on the surface of thrombus, so that superior stirring and mixing effect can be generated. Furthermore, as the flow velocity and shear stress on thrombus surface are both highly correlated with the thrombolysis effect (the correlation coefficient R1 = 0.988, R2 = 0.958, respectively), the peak value of lysis rate (increase by at least 5.02%) also occurred at 10°.

Conclusions: The current results demonstrated that, with appropriately determined incident angle, higher thrombolysis rate could be achieved without increasing the driving pressure. It may shed the light on future US thrombolysis planning strategy that, if combined with other advanced technologies (e.g., machine-learning-based image analysis and image-guided adaptive US emission modulation), more efficient thrombolytic effect could be realized while minimizing undesired side-effects caused by excessively high pressure.
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http://dx.doi.org/10.1002/mp.15874DOI Listing
July 2022

Colocalized targeting of TGF-β and PD-L1 by bintrafusp alfa elicits distinct antitumor responses.

J Immunother Cancer 2022 Jul;10(7)

Department of TIP OIO, EMD Serono Research and Development Institute, Billerica, Massachusetts, USA

Background: Bintrafusp alfa (BA) is a bifunctional fusion protein designed for colocalized, simultaneous inhibition of two immunosuppressive pathways, transforming growth factor-β (TGF-β) and programmed death-ligand 1 (PD-L1), within the tumor microenvironment (TME). We hypothesized that targeting PD-L1 to the tumor by BA colocalizes the TGF-β trap (TGF-βRII) to the TME, enabling it to sequester TGF-β in the tumor more effectively than systemic TGF-β blockade, thereby enhancing antitumor activity.

Methods: Multiple technologies were used to characterize the TGF-β trap binding avidity. BA versus combinations of anti-PD-L1 and TGF-β trap or the pan-TGF-β antibody fresolimumab were compared in proliferation and two-way mixed lymphocyte reaction assays. Immunophenotyping of tumor-infiltrating lymphocytes (TILs) and RNA sequencing (RNAseq) analysis assessing stromal and immune landscape following BA or the combination therapy were performed in MC38 tumors. TGF-β and PD-L1 co-expression and their associated gene signatures in MC38 tumors and human lung carcinoma tissue were studied with single-cell RNAseq (scRNAseq) and immunostaining. BA-induced internalization, degradation, and depletion of TGF-β were investigated in vitro.

Results: BA and fresolimumab had comparable intrinsic binding to TGF-β1, but there was an ~80× avidity-based increase in binding affinity with BA. BA inhibited cell proliferation in TGF-β-dependent and PD-L1-expressing cells more potently than TGF-β trap or fresolimumab. Compared with the combination of anti-PD-L1 and TGF-β trap or fresolimumab, BA enhanced T cell activation in vitro and increased TILs in MC38 tumors, which correlated with efficacy. BA induced distinct gene expression in the TME compared with the combination therapy, including upregulation of immune-related gene signatures and reduced activities in TGF-β-regulated pathways, such as epithelial-mesenchymal transition, extracellular matrix deposition, and fibrosis. Regulatory T cells, macrophages, immune cells of myeloid lineage, and fibroblasts were key PD-L1/TGF-β1 co-expressing cells in the TME. scRNAseq analysis suggested BA modulation of the macrophage phenotype, which was confirmed by histological assessment. PD-L1/TGF-β1 co-expression was also seen in human tumors. Finally, BA induced TGF-β1 internalization and degradation in the lysosomes.

Conclusion: BA more effectively blocks TGF-β by targeting TGF-β trap to the tumor via PD-L1 binding. Such colocalized targeting elicits distinct and superior antitumor responses relative to single agent combination therapy.
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http://dx.doi.org/10.1136/jitc-2021-004122DOI Listing
July 2022

FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.

Clin Transl Med 2022 Jul;12(7):e891

State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

Background: An impeccable female meiotic prophase is critical for producing a high-quality oocyte and, ultimately, a healthy newborn. SYCP3 is a key component of the synaptonemal complex regulating meiotic homologous recombination. However, what regulates SYCP3 stability is unknown.

Methods: Fertility assays, follicle counting, meiotic prophase stage (leptotene, zygotene, pachytene and diplotene) analysis and live imaging were employed to examine how FBXW24 knockout (KO) affect female fertility, follicle reserve, oocyte quality, meiotic prophase progression of female germ cells, and meiosis of oocytes. Western blot and immunostaining were used to examined the levels & signals (intensity, foci) of SYCP3 and multiple key DSB indicators & repair proteins (γH2AX, RPA2, p-CHK2, RAD51, MLH1, HORMAD1, TRIP13) after FBXW24 KO. Co-IP and immuno-EM were used to examined the interaction between FBXW24 and SYCP3; Mass spec was used to characterize the ubiquitination sites in SYCP3; In vivo & in vitro ubiquitination assays were utilized to determine the key sites in SYCP3 & FBXW24 for ubiquitination.

Results: Fbxw24-knockout (KO) female mice were infertile due to massive oocyte death upon meiosis entry. Fbxw24-KO oocytes were defective due to elevated DNA double-strand breaks (DSBs) and inseparable homologous chromosomes. Fbxw24-KO germ cells showed increased SYCP3 levels, delayed prophase progression, increased DSBs, and decreased crossover foci. Next, we found that FBXW24 directly binds and ubiquitinates SYCP3 to regulate its stability. In addition, several key residues important for SYCP3 ubiquitination and FBXW24 ubiquitinating activity were characterized.

Conclusions: We proposed that FBXW24 regulates the timely degradation of SYCP3 to ensure normal crossover and DSB repair during pachytene. FBXW24-KO delayed SYCP3 degradation and DSB repair from pachytene until metaphase II (MII), ultimately causing failure in oocyte maturation, oocyte death, and infertility.
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http://dx.doi.org/10.1002/ctm2.891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299759PMC
July 2022

TMT-based proteomic analysis of liquorice root in response to drought stress.

BMC Genomics 2022 Jul 19;23(1):524. Epub 2022 Jul 19.

College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010011, China.

Background: Drought stress is a serious threat to land use efficiency and crop yields worldwide. Understanding the mechanisms that plants use to withstand drought stress will help breeders to develop drought-tolerant medicinal crops. Liquorice (Glycyrrhiza uralensis Fisch.) is an important medicinal crop in the legume family and is currently grown mostly in northwest China, it is highly tolerant to drought. Given this, it is considered an ideal crop to study plant stress tolerance and can be used to identify drought-resistant proteins. Therefore, to understand the effects of drought stress on protein levels of liquorice, we undertook a comparative proteomic analysis of liquorice seedlings grown for 10 days in soil with different relative water content (SRWC of 80%, 65%, 50% and 35%, respectively). We used an integrated approach of Tandem Mass Tag labeling in conjunction with LC-MS/MS.

Results: A total of 7409 proteins were identified in this study, of which 7305 total proteins could be quantified. There were 837 differentially expressed proteins (DEPs) identified after different drought stresses. Compared with CK, 123 DEPs (80 up-regulated and 43 down-regulated) were found in LS; 353 DEPs (254 up-regulated and 99 down-regulated) in MS; and 564 DEPs (312 up-regulated and 252 down-regulated) in SS.The number of differentially expressed proteins increased with increasing water stress, and the number of up-regulated proteins was higher than that of down-regulated proteins in the different drought stress treatments compared with the CK. Used systematic bioinformatics analysis of these data to identify informative proteins we showed that osmolytes such as cottonseed sugars and proline accumulated under light drought stress and improved resistance. Under moderate and severe drought stress, oxidation of unsaturated fatty acids and accumulation of glucose and galactose increased in response to drought stress. Under moderate and severe drought stress synthesis of the terpene precursors, pentacene 2,3-epoxide and β-coumarin, was inhibited and accumulation of triterpenoids (glycyrrhetinic acid) was also affected.

Conclusions: These data provide a baseline reference for further study of the downstream liquorice proteome in response to drought stress. Our data show that liquorice roots exhibit specific response mechanisms to different drought stresses.
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http://dx.doi.org/10.1186/s12864-022-08733-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297632PMC
July 2022

Protocol to predict mechanical properties of multi-element ceramics using machine learning.

STAR Protoc 2022 Jul 18;3(3):101552. Epub 2022 Jul 18.

Department of Chemical & Materials Engineering, University of Alberta, Edmonton, AB T6G 2H5, Canada. Electronic address:

Identifying and designing high-performance multi-element ceramics based on trial-and-error approaches are ineffective and expensive. Here, we present a machine-learning-accelerated method for prediction of mechanical properties of multi-element ceramics, based on the density functional theory calculation database. Specific bonding characteristics are used as highly efficient machine learning descriptors. This protocol describes a low-cost, high-efficiency, and reliable workflow for developing advanced ceramics with superior mechanical properties. For complete details on the use and execution of this protocol, please refer to Tang et al. (2021).
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http://dx.doi.org/10.1016/j.xpro.2022.101552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304642PMC
July 2022

Motor functional recovery efficacy of scaffolds with bone marrow stem cells in rat spinal cord injury: a Bayesian network meta-analysis.

Spinal Cord 2022 Jul 16. Epub 2022 Jul 16.

Department of Orthopedic Surgery, The First Affliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Key Laboratory of Rheumatic Disease and Translational Medicine, Jinan, China.

Study Design: A Bayesian network meta-analysis.

Objective: Spinal cord injury (SCI) can profoundly influence human health and has been linked to lifelong disability. More high-level evidence-based medical research is expected to evaluate the value of stem cells and biomaterial scaffold material therapy for SCI.

Methods: We performed a comprehensive search of Web of Science, Cochrane databases, Embase, and PubMed databases. 18 randomized controlled trials including both scaffolds and BMSCs were included. We performed a Bayesian network meta-analysis to compare the motor functional recovery efficacy of different scaffolds with BMSCs in rat SCI.

Results: In our Bayesian network meta-analysis, the motor functional recovery was found to benefit from scaffolds, BMSCs, and BMSCs combined with scaffolds, but the scaffold and BMSC groups had similar motor functional recovery efficacy, and the BMSCs combined with scaffolds group appeared to show better efficacy than BMSCs and scaffolds alone. Subgroup analysis showed that BMSCs+fibrin, BMSCs+ASC, BMSCs+gelatine, and BMSCs+collagen were the best four treatments for SCI in rat models.

Conclusions: These Bayesian network meta-analysis findings strongly indicated that BMSCs combined with scaffolds is more effective to improve motor functional recovery than BMSCs and scaffolds alone. The fibrin, gelatine, ASC, and collagen may be favourable scaffolds for the injured spinal cord and that scaffolds with BMSCs could be a promising option in regeneration therapy for patients with SCI.
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http://dx.doi.org/10.1038/s41393-022-00836-4DOI Listing
July 2022

Binding properties of odorant-binding protein 4 from bean bug Riptortus pedestris to soybean volatiles.

Insect Mol Biol 2022 Jul 14. Epub 2022 Jul 14.

Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei, China.

The bean bug Riptortus pedestris is a notorious insect pest that can damage various crops, especially soybean, in East Asia. In insects, the olfactory system plays a crucial role in host finding and feeding behavior in which the odorant-binding proteins (OBPs) are believed to be involved in initial step in this system. In this study, we produced the R. pedestris adult antennae-expressed RpedOBP4 protein using a recombinant expression system in E. coli. Fluorescence competitive binding confirmed that RpedOBP4 has binding affinities to 7 of 20 soybean volatiles (ligands), and that a neutral condition is the best environment for it. The binding property of RpedOBP4 to these ligands was further revealed by integrating data from molecular docking, site-directed mutagenesis, and ligand binding assays. This demonstrated that five amino acid residues (I30, L33, Y47, I57, and Y121) are involved in the binding process of RpedOBP4 to corresponding ligands. These findings will not only help us to more thoroughly explore the olfactory mechanism of R. pedestris during feeding on soybean, but also lead to the identification of key candidate targets for developing environmental and efficient behavior inhibitors to prevent population expansion of R. pedestris in the future. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/imb.12802DOI Listing
July 2022

High-Dimensional Immune Profiling by Mass Cytometry Revealed the Circulating Immune Cell Landscape in Patients With Intracranial Aneurysm.

Front Immunol 2022 27;13:922000. Epub 2022 Jun 27.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Increasing evidence supports a critical role of chronic inflammation in intracranial aneurysm (IA). Understanding how the immunological alterations in IA provides opportunities for targeted treatment. However, there is a lack of comprehensive and detailed characterization of the changes in circulating immune cells in IA.

Objective: To perform a comprehensive and detailed characterization of the changes in circulating immune cells in patients with IA.

Methods: Peripheral blood mononuclear cell samples from IA patients (n = 26) and age-and sex-matched healthy controls (HCs, n = 20) were analyzed using high dimensional mass cytometry, and the frequency and phenotype of immune cell subtypes were assessed.

Results: We identified 28 cell clusters and found that the immune signature of IA consists of cluster changes. IA patients exhibited dysfunction of immunity, with dysregulation of CD4 T-cell clusters, increased B cells and monocytes, and decreased CD8 T cells, DNT cells, and DPT cells. Moreover, compared with findings in HC, IA was associated with enhanced lymphocyte and monocyte immune activation, with a higher expression of HLA-DR, CXCR3, and CX3CR1. In addition, the expression of TLR4, p-STAT3, and the exhaustion marker PD1 was increased in T cells, B cells, and NK cells in IA patients.

Conclusions: Our data provide an overview of the circulating immune cell landscape of IA patients, and reveal that the dysfunction of circulating immunity may play a potential role in the development of IA.
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http://dx.doi.org/10.3389/fimmu.2022.922000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271834PMC
June 2022

Injectable, Adhesive, and Self-Healing Composite Hydrogels Loaded With Oxybutynin Hydrochloride for the Treatment of Overactive Bladder in Rats.

Front Bioeng Biotechnol 2022 27;10:906835. Epub 2022 Jun 27.

Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

The aim of this study was to prepare injectable, adhesive, and self-healing composite hydrogels loaded with oxybutynin hydrochloride and verify its function in the treatment of overactive bladder. The ultraviolet (UV) absorption of oxybutynin (Oxy) in the solution was detected using a UV spectrophotometer at 233 nm, and the cumulative drug release was calculated using Origin software. L929 mouse fibroblasts were used to test cell adhesion to OCP50 and OCP100 hydrogels. Both FT-IR and NMR overactive bladder demonstrated that Dex was oxidized to PDA with aldehyde groups. Urodynamic examinations were performed 24 h after intraperitoneal injection in the rat model. The relative expression levels of Orai1 and STIM1 were detected by western blot (WB) and QPCR. After loading Oxy, the shear adhesion under the wet conditions of OCP50 and OCP100 was higher than CP50 and CP100 ( < 0.05), and both were suitable for intravaginal administration. After 72 h of release, oxybutynin released 82.8% in OCP100 hydrogel and 70% in OCP50. Compared to the model, OCP50, CP100, and OCP100 relieved the overactive bladder and inhibited the expression of Orail and STIM1. Oxybutynin hydrogel could provide relief to overactive bladder by decreasing the expression of Orail and STIM1 in rats.
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http://dx.doi.org/10.3389/fbioe.2022.906835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272884PMC
June 2022
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