Publications by authors named "Dong Wang"

3,776 Publications

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Using weakly supervised deep learning to classify and segment single-molecule break-junction conductance traces.

Chemphyschem 2021 Jul 29. Epub 2021 Jul 29.

Peking University, No.5, Yiheyuan Road, Haidian District, 100871, Beijing, CHINA.

In order to design molecular electronic devices with high performance and stability, it is crucial to understand their structure-to-property relationships. Single-molecule break junction measurements yield a large number of conductance-distance traces, which are inherently highly stochastic. Here we propose a weakly supervised deep learning algorithm to classify and segment these conductance traces, a method that is mainly based on transfer learning with the pretrain-finetune technique. By exploiting the powerful feature extraction capabilities of the pretrained VGG-16 network, our convolutional neural network model not only achieves high accuracy in the classification of the conductance traces, but also segments precisely the conductance plateau from an entire trace with very few manually labeled traces. Thus, we can produce a more reliable estimation of the junction conductance and quantify the junction stability. These findings show that our model has achieved a better accuracy-to-manpower efficiency balance, opening up the possibility of using weakly supervised deep learning approaches in the studies of single-molecule junctions.
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http://dx.doi.org/10.1002/cphc.202100414DOI Listing
July 2021

Rotational diffusion and rotational correlations in frictional amorphous disk packings under shear.

Soft Matter 2021 Jul 29. Epub 2021 Jul 29.

Physical Chemistry and Soft Matter, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.

We show here that rotations of round particles in amorphous disk packing reveal various nontrivial microscopic features when the packing is close to rigidification. We analyze experimental measurements on disk packing subjected to simple shear deformation with various inter-particle friction coefficients and across a range of volume fractions where the system is known to stiffen. The analysis of measurements indicates that shear induces diffusive microrotation, that can be both enhanced and suppressed depending upon the volume fraction as well as the inter-particle friction. Rotations also display persistent anticorrelated motion. Spatial correlations in microrotation are observed to be directly correlated with system pressure. These observations point towards the broader mechanical relevance of collective dynamics in the rotational degree of freedom of particles.
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http://dx.doi.org/10.1039/d1sm00525aDOI Listing
July 2021

[Case control study on T-plate combined with suture anchors for the treatment of Neer Ⅱb clavicle fractures].

Zhongguo Gu Shang 2021 Jul;34(7):679-83

Department of Orthopaedics, Wuwei People's Hosptial, Wuwei 733000, Gansu, China.

Objective: To compare the clinical efficacy of distal radius T-plate combined with suture anchor and distal clavicle anatomical locking plate combined with suture anchor in the treatment of Neer Ⅱb distal clavicle fracture.

Methods: From June 2014 to June 2018, 42 patients with Neer Ⅱb distal clavicle fractures were retrospectively analyzed. According to different surgical methods, they were divided into the observation group (T-shaped plate combined with suture anchor) and the control group (anatomical locking plate combined with suture anchor). There were 22 patients in the observation group and 20 patients in the control group. In the observation group, there were 13 males and 9 females, aged from 22 to 70 (45.78± 14.44) years old, 12 cases on the left side and 10 cases on the right side, 8 cases of traffic accident injury and 14 cases of fall. In the control group, there were 12 males and 8 females, aged from 24 to 66 (44.17±15.58) years, 13 cases on the left side and 7 cases on the right side, 6 cases of traffic accident injuryand 14 cases of fall. The operation time, intraoperative blood loss and fracture healing time were compared between the two groups, and Constant Murley score was used to evaluate shoulder joint function.

Results: The patients in both groups were followed up for 18 to 24 (20.96±2.02) months. The incisions of both groups were healed at stageⅠ. The fracture ends of both groups were bony healed at the last follow up. There was no significant difference in operation time, intraoperative blood loss and fracture healing time between two groups (>0.05);there was no significant difference in shoulder joint function between two groups at 3 months after operation (>0.05).

Conclusion: The two methods can obtain satisfactory results in the treatment of Neer Ⅱb distal clavicle fractures, especially suitable for patients with comminuted distal clavicle fractures or osteoporosis; the clinical effect of the treatment of NeerⅡb distal clavicle fractures with T type distal radius plate combined with suture anchor is satisfactory, which provides another feasible treatment scheme for clinic.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.07.018DOI Listing
July 2021

Fabrics Attached with Highly Efficient Aggregation-Induced Emission Photosensitizer: Toward Self-Antiviral Personal Protective Equipment.

ACS Nano 2021 Jul 27. Epub 2021 Jul 27.

Center for Infection and Immunity, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, Guangdong, China.

Personal protective equipment (PPE) is vital for the prevention and control of SARS-CoV-2. However, conventional PPEs lack virucidal capabilities and arbitrarily discarding used PPEs may cause a high risk for cross-contamination and environmental pollution. Recently reported photothermal or photodynamic-mediated self-sterilizing masks show bactericidal-virucidal abilities but have some inherent disadvantages, such as generating unbearable heat during the photothermal process or requiring additional ultraviolet light irradiation to inactivate pathogens, which limit their practical applications. Here, we report the fabrication of a series of fabrics (derived from various PPEs) with real-time self-antiviral capabilities, on the basis of a highly efficient aggregation-induced emission photosensitizer (namely, ASCP-TPA). ASCP-TPA possesses facile synthesis, excellent biocompatibility, and extremely high reactive oxygen species generation capacity, which significantly outperforms the traditional photosensitizers. Meanwhile, the ASCP-TPA-attached fabrics (ATaFs) show tremendous photodynamic inactivation effects against MHV-A59, a surrogate coronavirus of SARS-CoV-2. Upon ultralow-power white light irradiation (3.0 mW cm), >99.999% virions (5 log) on the ATaFs are eliminated within 10 min. Such ultralow-power requirement and rapid virus-killing ability enable ATaFs-based PPEs to provide real-time protection for the wearers under indoor light irradiation. ATaFs' virucidal abilities are retained after 100 washings or continuous exposure to office light for 2 weeks, which offers the benefits of reusability and long-term usability. Furthermore, ATaFs show no toxicity to normal skin, even upon continuous high-power light illumination. This self-antiviral ATaFs-based strategy may also be applied to fight against other airborne pathogens and holds huge potential to alleviate global PPE supply shortages.
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http://dx.doi.org/10.1021/acsnano.1c06071DOI Listing
July 2021

Effects of long-term phosphorus application on crop yield, phosphorus absorption, and soil phosphorus accumulation in maize-wheat rotation system.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2469-2476

College of Resources and Environmental Science/Hebei Province Key Laboratory for Farmland Eco-environment, Hebei Agricultural University, Baoding 071000, Hebei, China.

In this study, we examined the effects of four phosphorus treatments on crop yields, and analyzed crop phosphorus uptake and phosphorus utilization efficiency, as well as changes in soil phosphorus deficit in maize-wheat rotation system, based on a 7-year field experiment in Baoding City, Hebei Province. The results showed that long-term phosphorus application significantly increased the yield and phosphorus uptake of maize and wheat. The yield and phosphorus uptake of maize and wheat showed a parabolic trend that first increased and then decreased with the increases of phosphorus application. The utilization efficiency of phosphorus fertilizer in the corn season was higher than that in the wheat season under various phosphorus application treatments. The cumulative utilization efficiency of phosphorus fertilizer in the wheat season showed a downward trend. The decline rate showed that optimized phosphorus application > 70% optimized phosphorus application >130% optimized phosphorus application. The cumulative utilization efficiency of phosphorus fertili-zer in the corn season showed an upward trend. For the rising rate, the optimized phosphorus application > 70% optimized phosphorus application > 130% optimized phosphorus application. Both the apparent phosphorus surplus and the accumulated phosphorus surplus of the soil without phosphorus application were under phosphorus deficiency. Under the treatment of phosphorus application, soil phosphorus showed a status of phosphorus surplus. The higher the phosphorus application rate, the longer the accumulation period, the higher the soil phosphorus surplus. Under the straw returning condition, phosphorus application rate for wheat of 105-150 kg·hm and the phosphorus application rate for maize of 63-90 kg·hm in Hebei fluvo-aquic soil could ensure the high crop yield, keep the phosphorus utilization efficiency at a high level, and reduce soil phosphorus accumulation and environmental risks.
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http://dx.doi.org/10.13287/j.1001-9332.202107.026DOI Listing
July 2021

Efficacy and safety of induction chemotherapy with daunorubicin or idarubicin in the treatment of an adult with acute lymphoblastic leukemia.

Tumori 2021 Jul 23:3008916211032724. Epub 2021 Jul 23.

Department of Hematology and Rheumatology, The Second Affiliated Hospital of Mudanjiang University, Mudanjiang, China.

Background: Anthracyclines are dispensable components of chemotherapy of patients with acute lymphoblastic leukemia (ALL).

Objective: To analyze the efficacy and safety of induction with idarubicin (IDA) or liposoma daunorubicin (L-DNR) in treatment of adults with high-risk ALL (HR-ALL) (presence of mixed lineage leukemia gene [] rearrangements, t[1;19], or prednisone poor response).

Methods: Among 58 enrolled patients, 29 cases were defined as the IDA group and the other 29 patients were put into the L-DNR group. Both overall survival (OS) and progression-free survival (PFS) were estimated and overall response rate (ORR) was compared between the groups.

Results: The L-DNR group's OS and PFS were insignificantly higher than in the IDA group (=0.261 and =0.247). Although not significantly different, the ORR of adults with HR-ALL receiving L-DNR regimens was also higher than in the IDA group (=0.085). Comprehensive cytogenetic analysis revealed that patients harboring rearrangement, , and mutation had poorer prognosis than others. All 58 patients experienced hematologic response in this study; however, the length of hematologic response in the IDA group was significantly longer than in the L-DNR group (=0.005). The incidence of bleeding and infection was without significant difference between the groups (both >0.05).

Conclusion: L-DNR proved to be an effective drug within a multiagent approach, which shows a favorable overall profile, as well as similar adverse events when compared with IDA in HR-ALL. Patients with are much more sensitive to L-DNR than IDA. Despite some progress made, outcomes in rearrangement or mutation carriers remain unsatisfactory, and intensive treatment will be critical.
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http://dx.doi.org/10.1177/03008916211032724DOI Listing
July 2021

Side Area-Assisted 3D Evaporator with Antibiofouling Function for Ultra-Efficient Solar Steam Generation.

Adv Mater 2021 Jul 26:e2102258. Epub 2021 Jul 26.

Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, 999077, China.

Solar-driven interfacial steam generation (SISG) has been recognized as a promising strategy to solve water shortages in an eco-friendly and low-cost way. However, the practical application of SISG is vitally restricted by some inherent limits, especially for finite evaporation rate and insufficient working life of evaporator. Herein, a novel SISG system involving an all-fiber porous cylinder-like foam 3D evaporator, side area-assisted evaporation protocol, and aggregation-induced-emission-active solar absorber with "one stone two birds" function is explored. The solar absorber exhibits efficient photothermal conversion, endowing the side area-assisted evaporator with as high as 3.6 kg m h of solar evaporation rate, which is highly desirable for SISG under 1 sun of irradiation. Moreover, the solar absorber is capable of powerfully producing reactive oxygen species upon sunlight irradiation, which results in extraordinary photodynamic killing of bacteria nearby the fiber to prevent biofouling, consequently improving the working life of evaporator.
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http://dx.doi.org/10.1002/adma.202102258DOI Listing
July 2021

Malectin/Malectin-like domain-containing proteins: A repertoire of cell surface molecules with broad functional potential.

Cell Surf 2021 Dec 24;7:100056. Epub 2021 Jun 24.

Department of Biochemistry and Molecular Biology, University of Massachusetts, USA.

Cell walls are at the front line of interactions between walled-organisms and their environment. They support cell expansion, ensure cell integrity and, for multicellular organisms such as plants, they provide cell adherence, support cell shape morphogenesis and mediate cell-cell communication. Wall-sensing, detecting perturbations in the wall and signaling the cell to respond accordingly, is crucial for growth and survival. In recent years, plant signaling research has suggested that a large family of receptor-like kinases (RLKs) could function as wall sensors partly because their extracellular domains show homology with malectin, a diglucose binding protein from the endoplasmic reticulum of animal cells. Studies of several malectin/malectin-like (M/ML) domain-containing RLKs (M/MLD-RLKs) from the model plant have revealed an impressive array of biological roles, controlling growth, reproduction and stress responses, processes that in various ways rely on or affect the cell wall. Malectin homologous sequences are widespread across biological kingdoms, but plants have uniquely evolved a highly expanded family of proteins with ML domains embedded within various protein contexts. Here, we present an overview on proteins with malectin homologous sequences in different kingdoms, discuss the chromosomal organization of Arabidopsis M/MLD-RLKs and the phylogenetic relationship between these proteins from several model and crop species. We also discuss briefly the molecular networks that enable the diverse biological roles served by M/MLD-RLKs studied thus far.
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http://dx.doi.org/10.1016/j.tcsw.2021.100056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287233PMC
December 2021

Rapid global path planning algorithm for unmanned surface vehicles in large-scale and multi-island marine environments.

PeerJ Comput Sci 2021 29;7:e612. Epub 2021 Jun 29.

School of Electronics and Information Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, China.

A global path planning algorithm for unmanned surface vehicles (USVs) with short time requirements in large-scale and complex multi-island marine environments is proposed. The fast marching method-based path planning for USVs is performed on grid maps, resulting in a decrease in computer efficiency for larger maps. This can be mitigated by improving the algorithm process. In the proposed algorithm, path planning is performed twice in maps with different spatial resolution (SR) grids. The first path planning is performed in a low SR grid map to determine effective regions, and the second is executed in a high SR grid map to rapidly acquire the final high precision global path. In each path planning process, a modified inshore-distance-constraint fast marching square (IDC-FM) method is applied. Based on this method, the path portions around an obstacle can be constrained within a region determined by two inshore-distance parameters. The path planning results show that the proposed algorithm can generate smooth and safe global paths wherein the portions that bypass obstacles can be flexibly modified. Compared with the path planning based on the IDC-FM method applied to a single grid map, this algorithm can significantly improve the calculation efficiency while maintaining the precision of the planned path.
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http://dx.doi.org/10.7717/peerj-cs.612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279141PMC
June 2021

Long Non-coding RNA ASNR Targeting miR-519e-5p Promotes Gastric Cancer Development by Regulating FGFR2.

Front Cell Dev Biol 2021 9;9:679176. Epub 2021 Jul 9.

The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

Gastric cancer (GC), as a common gastrointestinal tumor, is an important cause of death from cancer all around the world. Long non-coding RNAs (lncRNAs), a novel class of transcripts, have attracted great attention of researchers. However, the mechanisms of the clinical significance of most lncRNAs in human cancer are mainly undocumented. This research desires to explore the clinical significance, biological function, and mechanism of Lnc_ASNR (apoptosis suppressing-non-coding RNA) in GC. Cell proliferation, cell cycle, cell migration, and invasion abilities were respectively determined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT), flow cytometry, wound healing, and Transwell assay (Sigma-Aldrich, St. Louis, MO, United States). The association of Lnc_ASNR, miR-519e-5p, and fibroblast growth factor receptor 2 (FGFR2) was evaluated luciferase reporter experiments. The tumor xenograft assay was conducted to confirm the results of cell experiments. High expressed Lnc_ASNR was detected in both GC cells and tissues using qRT-PCR. Downregulated Lnc_ASNR could reduce proliferation, migration, and invasion in GC cells, while upregulated Lnc_ASNR could promote the cell proliferation, migration, and invasion. Moreover, the effect of Lnc_ASNR on migration and invasion ability is closely related to epithelial-mesenchymal transition (EMT). The bioinformatics analysis, luciferase assay, and Western blot demonstrated that Lnc_ASNR inhibited miR-519e-5p expression but increased FGFR2 expression. Lnc_ASNR and FGFR2 were both targeted to miR-519e-5p, and they were negatively correlated with the expression of miR-519e-5p. All investigations indicated that Lnc_ASNR functioned as a ceRNA targeting miR-519e-5p and facilitated GC development by regulating the pathway of miR-519e-5p/FGFR2.
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http://dx.doi.org/10.3389/fcell.2021.679176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299726PMC
July 2021

Early and late outcomes of non-total aortic arch replacement for repair of acute Stanford Type A aortic dissection.

Am J Transl Res 2021 15;13(6):7047-7052. Epub 2021 Jun 15.

Department of Cardiovascular Surgery, Shanxi Bethune Hospital (Shanxi Academy of Medical Sciences) Taiyuan 030032, Shanxi, China.

Objective: This study evaluated the early and late outcomes of non-total aortic arch replacement for acute Stanford A aortic dissection.

Methods: 131 cases of acute Stanford Type A aortic dissection with no rupture admitted to our hospital from January 2016 to December 2019 were selected for non-total aortic arch replacement. According to different surgical methods, 51 patients with tear-oriented ascending/hemiarch replacement were included in Group A, and 80 patients who underwent total arch replacement surgery were enrolled in Group B. The perioperative indicators, 30-day mortality rate, and the incidence of postoperative complications were compared between the two groups, and the survival rate of patients were compared by follow-up after discharge.

Results: The cardiopulmonary bypass time, cardiac perfusion time, invasive ventilation and ICU hospitalization in Group A were critically shorter than those in Group B (). The incidence of transient cerebral dysfunction in Group A was substantially lower than that in Group B (). The difference of comparison in perioperative mortality, incidence of permanent neurological dysfunction, and incidence of acute kidney and liver damage between the two groups was statistically insignificant (). In addition, the two groups had statistically insignificant difference in survival during postoperative follow-up ().

Conclusion: For acute Stanford type A aortic dissection without rupture in aortic arch, the non-total aortic arch replacement has simple surgical method with high perioperative safety and long-term efficacy that similar to total arch replacement.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290664PMC
June 2021

Risk factors of postoperative ileus following laparoscopic radical cystectomy and developing a points-based risk assessment scale.

Transl Androl Urol 2021 Jun;10(6):2397-2409

Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Dongcheng, Beijing, China.

Background: Postoperative ileus (POI) is one of the most common complications after laparoscopic radical cystectomy (LRC). Albeit its high incidence, its risk factors are obscure, and few studies have attempted to explore them. Meanwhile, risk-assessing tools for predicting its happening are lacking.

Methods: Clinical data of 197 patients who underwent LRC between March 2014 and October 2019 were retrospectively collected. All cases of POI were identified and double-checked. Data pertaining to the following categories were extracted as well: patients' general characteristics, preoperative laboratory tests results and preparations, intraoperative and postoperative general items, pathological results. The correlation between candidate risk factors and ileus was analyzed by multivariable binary logistic regression. Clinical and pathophysiological explanations for those results were explored. Finally, a points-based prediction model was developed and validated for predicting the happening of POI.

Results: A total of 63 out of 197 patients (31.98%) suffered from POI. Multivariate logistic regression analysis showed chronic constipation, increased dosage of laxative, elevated preoperative serum creatinine level, delayed postoperative ambulation, intestine-related urine derivations were statistically significant for developing POI (P<0.05). No significant differences were found between POI and age, gender, body mass index (BMI), antibiotics, hypertension, diabetes, smoking, hard-drinking, preoperative hemoglobin level, preoperative albumin level, history of previous abdominal surgery, surgery time, intraoperative blood loss, blood transfusion, tumor size, lymph nodes yields, TNM staging and intensive care unit hospitalization. An external cohort had been used for testing the validation of the assessment scale, and the results were promising.

Conclusions: Early recognition is of great importance in protecting vulnerable patients from developing POI, knowing the above-mentioned risk factors and using the assessment scale should help to screen them better. Cases from diverse backgrounds might contribute to a more accurate and complete scale.
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http://dx.doi.org/10.21037/tau-21-112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261424PMC
June 2021

The Emergence and Molecular Characteristics of New Delhi Metallo -Lactamase-Producing From Ducks in Guangdong, China.

Front Microbiol 2021 5;12:677633. Epub 2021 Jul 5.

National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.

This study aimed to determine the prevalence and transmission characteristics of New Delhi metallo β-lactamase (NDM)-producing from ducks in Guangdong, China. In this study, a total of 28 NDM-producing isolates were recovered from 88 unduplicated diseased duck samples (31.8%) from veterinary clinics in Guangzhou, Foshan, Qingyuan, and Huizhou. Two variants, and , were detected and the latter was present in 89.6% of the isolates (25/28). Multilocus sequence typing (MLST) analysis indicated that these isolates possessed six distinct STs, and ST156 was the most prevalent followed by ST648, ST746, ST354, ST10, and ST162. In addition, phylogenomic analysis found that two of the isolates that were recovered from a single sample possessed different genomes, and the -carrying IncX3 plasmids may be horizontal transfer between isolates in the intestinal tracts of ducks. Whole-genome sequencing (WGS) analysis further revealed that co-existed with other 25 types of antimicrobial resistance genes (ARGs), of which 16 ARGs were highly prevalent with detection rates >50%, and a high incidence of coproducing and isolates (22/88, 25.0%) was detected in ducks. This study underscores the importance of surveillance for -harboring microbes in ducks.
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http://dx.doi.org/10.3389/fmicb.2021.677633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287858PMC
July 2021

SAR1B senses leucine levels to regulate mTORC1 signalling.

Nature 2021 Jul 21. Epub 2021 Jul 21.

State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing, China.

The mTOR complex 1 (mTORC1) controls cell growth in response to amino acid levels. Here we report SAR1B as a leucine sensor that regulates mTORC1 signalling in response to intracellular levels of leucine. Under conditions of leucine deficiency, SAR1B inhibits mTORC1 by physically targeting its activator GATOR2. In conditions of leucine sufficiency, SAR1B binds to leucine, undergoes a conformational change and dissociates from GATOR2, which results in mTORC1 activation. SAR1B-GATOR2-mTORC1 signalling is conserved in nematodes and has a role in the regulation of lifespan. Bioinformatic analysis reveals that SAR1B deficiency correlates with the development of lung cancer. The silencing of SAR1B and its paralogue SAR1A promotes mTORC1-dependent growth of lung tumours in mice. Our results reveal that SAR1B is a conserved leucine sensor that has a potential role in the development of lung cancer.
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http://dx.doi.org/10.1038/s41586-021-03768-wDOI Listing
July 2021

Removal behavior and chemical speciation distributions of heavy metals in sewage sludge during bioleaching and combined bioleaching/Fenton-like processes.

Sci Rep 2021 Jul 21;11(1):14879. Epub 2021 Jul 21.

School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.

The removal and chemical speciation changes of heavy metals in the sewage sludge during the single bioleaching and combined bioleaching/Fenton-like processes were compared in this study. The improvement in the dewaterability of the treated sludge was also investigated. The single bioleaching led to a removal of Zn, Cu, Cd, Cr, Mn, Ni, As and Pb of 67.28%, 50.78%, 64.86%, 6.32%, 56.15%, 49.83%, 20.78% and 10.52% in 10 days, respectively. The chemical speciation analysis showed that the solubilization of heavy metals in mobile forms (exchangeable/acid soluble and reducible forms) and oxidizable form was the main reason for their removal. Subsequent Fenton-like treatment was carried out at different bioleaching stages when the bioleached sludge dropped to certain pH values (4.5, 4.0 and 3.0), by adding HO at different dosages. The highest removal ratio of Zn, Cu, Cd, Cr, Mn and Ni could reach 75.53%, 52.17%, 71.91%, 11.63%, 66.29% and 65.19% after combined bioleaching/Fenton-like process, respectively, with appropriate pH and HO dosages in less than 6 days. The solubilization efficiencies of these heavy metals in mobile forms were further improved by Fenton-like treatment. The removal efficiencies of As and Pb decreased due to their transformation into insoluble forms (mostly residual fraction) after Fenton treatment. The capillary suction times (CST) of the raw sludge (98.7 s) decreased by 79.43% after bioleaching and 87.44% after combined process, respectively.
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http://dx.doi.org/10.1038/s41598-021-94216-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295269PMC
July 2021

Development of autotrophic and heterotrophic consortia via immobilized microbial beads for chemical wastewater treatment, using PTA wastewater as an approach.

Chemosphere 2021 Oct 26;281:131001. Epub 2021 May 26.

China Pharmaceutical University, Nanjing, 211198, China. Electronic address:

Studies on the symbiosis of microalgae-bacteria have been accelerating as a mean for wastewater remediation. However, there were few reports about the microalgae-bacteria consortia for chemical wastewater treatment. The aim of the present study is to develop an autotrophic and heterotrophic consortium for chemical wastewater treatment and probe whether and how bacteria could benefit from the microalgae during the treatment process, using PTA wastewater as an approach. A process-dependent strategy was applied. First of all, the results showed that the sludge beads with the sludge concentration of 30 g/L were the optimal one with the COD removal rate at 84.8% but the ceiling effect occurred (COD removal rate < 90%) even several common reinforcement methods were applied. Additionally, by adding the microalgae Chlorella vulgaris, a microalgae-activated sludge consortium was formed inside the immobilized beads, which provided better performance to shatter the ceiling effect. The COD remove rate was higher than 90%, regardless of the activated sludge was pre-culture or not. COD removal capacity could also be improved (COD removal rate > 92%) when LEDs light belt was offered as an advanced light condition. Biochemical assay and DNA analysis indicated that the microalgae could form an internal circulation of substances within the activated sludge and drove the microbial community to success and the corresponding gene functions, like metabolism and.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131001DOI Listing
October 2021

A core-shell [email protected] nanocatalyst under ozone activation for efficient organic degradation: Surface-mediated non-radical oxidation.

Chemosphere 2021 Oct 17;281:130895. Epub 2021 May 17.

School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, China. Electronic address:

A non-radical catalytic ozonation process functionalized by Mn(II)-sites was found effective to degrade oxalic acid as a typical refractory compound that is hard to remove through catalytic ozonation with hydroxyl radical oxidation. Specifically, we prepared functional [email protected] nanoparticles with carbon shell supporting MnO for catalysis while encapsulating magnetic iron core for recycle. Batch study shows its excellent catalytic reactivity on the degradation of organics particular for the ozone-recalcitrant oxalic acid (20 min for 90.9% removal, kinetics constant of 0.045 min). Our mechanism study ruled out the hydroxyl radical oxidation, and highlighted the formation of a heptavalent Mn intermediate oxidant responsible for the fast degradation that was initiated via ozone activation of Mn(II)-sites on the carbon shell. Our findings underscore a non-radical catalytic ozonation as a promising alternative technology for the abatement of aqueous organic pollutants.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130895DOI Listing
October 2021

Comparative serum microRNA array analysis of the spleen-stomach dampness-heat syndrome in different diseases: Chronic hepatitis B and chronic gastritis.

Anat Rec (Hoboken) 2021 Jul 20. Epub 2021 Jul 20.

College of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Spleen-stomach dampness-heat syndrome (SSDHS) is the common Traditional Chinese Medicine (TCM) syndrome observed in both chronic hepatitis B (CHB) and chronic gastritis (CG). The specialized TCM prescription for CHB and CG patients with SSDHS is same, but there is limited information about the biological characteristics of this TCM syndrome. This study aimed to identify the serum miRNAs profile for the SSDHS in two different diseases in order to evaluate the miRNA-mediated biological characteristics of this TCM syndrome. We performed comparative microarray analysis of serum miRNA expression profiles in 10 CHB patients with SSDHS (SSDHS-CHB), 10 CG patients with SSDHS (SSDHS-CG), and 10 healthy controls (HC). The selected miRNAs were further validated by qRT-PCR in 13 SSDHS-CHB patients, 13 SSDHS-CG patients, and 13 HC. Moreover, bioinformatics analysis (GO and KEGG pathway enrichment analyses) was applied to identify the involved target genes and pathways for these selected miRNAs. Nine significantly differentially expressed (SDE)-miRNAs in the SSDHS-CHB group and 24 SDE-miRNAs in the SSDHS-CG group were identified, compared with the HC group (fold change >2.0 and p < .05). Among these, upregulated hsa-miR-483-3p and downregulated hsa-miR-223-3p were identified as the common SDE-miRNAs for both SSDHS-CHB and SSDHS-CG groups. Bioinformatics analysis of the common SDE-miRNA's target genes showed their involvement in the regulation of inflammation, immune response, and tumorigenesis. SSDHS-specific hsa-miR-483-3p and hsa-miR-223-3p identified in this study indicated a relevance to the underlying biological basis of SSDHS, and may provide scientific basis for the application of same TCM prescription in CHB and CG.
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http://dx.doi.org/10.1002/ar.24690DOI Listing
July 2021

TEM8 marks neovasculogenic tumor-initiating cells in triple-negative breast cancer.

Nat Commun 2021 07 20;12(1):4413. Epub 2021 Jul 20.

Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences; Cancer Institutes; Key Laboratory of Breast Cancer in Shanghai; The Shanghai Key Laboratory of Medical Epigenetics; The International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology; Shanghai Medical College, Fudan University, Shanghai, China.

Enhanced neovasculogenesis, especially vasculogenic mimicry (VM), contributes to the development of triple-negative breast cancer (TNBC). Breast tumor-initiating cells (BTICs) are involved in forming VM; however, the specific VM-forming BTIC population and the regulatory mechanisms remain undefined. We find that tumor endothelial marker 8 (TEM8) is abundantly expressed in TNBC and serves as a marker for VM-forming BTICs. Mechanistically, TEM8 increases active RhoC level and induces ROCK1-mediated phosphorylation of SMAD5, in a cascade essential for promoting stemness and VM capacity of breast cancer cells. ASB10, an estrogen receptor ERα trans-activated E3 ligase, ubiquitylates TEM8 for degradation, and its deficiency in TNBC resulted in a high homeostatic level of TEM8. In this work, we identify TEM8 as a functional marker for VM-forming BTICs in TNBC, providing a target for the development of effective therapies against TNBC targeting both BTIC self-renewal and neovasculogenesis simultaneously.
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http://dx.doi.org/10.1038/s41467-021-24703-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292527PMC
July 2021

Repetitive brief ischemia accelerates tibial shaft fracture healing: a 5-years prospective preliminary clinical trial (PCT).

BMC Musculoskelet Disord 2021 Jul 20;22(1):631. Epub 2021 Jul 20.

Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China.

Background: This study was to evaluate the effects of repetitive brief ischemia (RBI) on bone healing in patients with tibial shaft fractures.

Methods: In this prospective clinical trial, patients with tibia shaft fractures were enrolled between January 2016 and January 2021. The intermittent pneumatic compression (IPC) device was used to make RBI on the affected limb after surgical operation 24 h. The inflation pressure was the systolic pressure of patients + 50 mmHg. Patients were received 30 s inflation/30 s deflation 30 times twice a day for 4 weeks. The primary outcome was bone healing time and the secondary outcomes were the rates of delayed union and nonunion, the rates of venous thrombosis of lower limbs, Johner-Wruhs scores, Lysholm knee score, VAS scores, postoperative complications, serum BMP-2, osteocalcin (OC) and bone specific alkaline phosphatase (BS-ALP).

Results: A total of 32 patients were enrolled finally and all were completed with a 12 months follow-up. All the fractures were healed and the bone healing time was 3(1) months in RBI group. However, the bone healing time of control group was 4(1) and there was statistical difference between the two groups (p < 0.01). No patient with fracture delayed union, nonunion and venous thrombosis of lower limbs in RBI group. 2 patients were delayed union in the control group. In RBI group, the Lysholm knee score was 83(6) at 6 months and 100(8) at 12 months. In the control group, the score was 78(4) and 90.5(17), there was statistical difference between the two groups (p < 0.01, p = 0.014, respectively). VAS scores were postoperative 2 weeks 6(1) in RBI group and 7(0.5) in the control group, there was statistical difference between the two groups (p = 0.016). There were 2 patients with intramuscular venous thrombosis of lower extremity in control group. Besides, RBI treatment increased the serum BMP-2, OC and BS-ALP at postoperative 2 weeks and 1 month.

Conclusions: RBI is a new method to accelerate bone healing in tibia shaft fracture patients and is a simple and noninvasive method.

Trial Registration: Chinese clinical trial registry, ChiCTR-INR-17014208 . Registered 28 December 2017-Retrospectively registered.
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http://dx.doi.org/10.1186/s12891-021-04515-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293516PMC
July 2021

A Nomogram Based on Preoperative Inflammatory Indices and ICG-R15 for Prediction of Liver Failure After Hepatectomy in HCC Patients.

Front Oncol 2021 2;11:667496. Epub 2021 Jul 2.

Department of General Surgery, The Xiangya Hospital of Central South University, Changsha, China.

Objective: To establish a nomogram based on inflammatory indices and ICG-R15 for predicting post-hepatectomy liver failure (PHLF) among patients with resectable hepatocellular carcinoma (HCC).

Methods: A retrospective cohort of 407 patients with HCC hospitalized at Xiangya Hospital of Central South University between January 2015 and December 2020, and 81 patients with HCC hospitalized at the Second Xiangya Hospital of Central South University between January 2019 and January 2020 were included in the study. Totally 488 HCC patients were divided into the training cohort (n=378) and the validation cohort (n=110) by random sampling. Univariate and multivariate analysis was performed to identify the independent risk factors. Through combining these independent risk factors, a nomogram was established for the prediction of PHLF. The accuracy of the nomogram was evaluated and compared with traditional models, like CP score (Child-Pugh), MELD score (Model of End-Stage Liver Disease), and ALBI score (albumin-bilirubin) by using receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA).

Results: Cirrhosis (OR=2.203, 95%CI:1.070-3.824, P=0.030), prothrombin time (PT) (OR=1.886, 95%CI: 1.107-3.211, P=0.020), tumor size (OR=1.107, 95%CI: 1.022-1.200, P=0.013), ICG-R15% (OR=1.141, 95%CI: 1.070-1.216, P<0.001), blood loss (OR=2.415, 95%CI: 1.306-4.468, P=0.005) and AST-to-platelet ratio index (APRI) (OR=4.652, 95%CI: 1.432-15.112, P=0.011) were independent risk factors of PHLF. Nomogram was built with well-fitted calibration curves on the of these 6 factors. Comparing with CP score (C-index=0.582, 95%CI, 0.523-0.640), ALBI score (C-index=0.670, 95%CI, 0.615-0.725) and MELD score (C-ibasedndex=0.661, 95%CI, 0.606-0.716), the nomogram showed a better predictive value, with a C-index of 0.845 (95%CI, 0.806-0.884). The results were consistent in the validation cohort. DCA confirmed the conclusion as well.

Conclusion: A novel nomogram was established to predict PHLF in HCC patients. The nomogram showed a strong predictive efficiency and would be a convenient tool for us to facilitate clinical decisions.
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http://dx.doi.org/10.3389/fonc.2021.667496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283414PMC
July 2021

Efficiency and Toxicity of Ruxolitinib as a Salvage Treatment for Steroid-Refractory Chronic Graft-Versus-Host Disease.

Front Immunol 2021 30;12:673636. Epub 2021 Jun 30.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.

Graft-versus-host disease (GVHD), especially steroid-refractory GVHD, remains a life-threatening complication after hematopoietic stem cell transplantation (HSCT). The effect of the JAK1/2 kinase inhibitor ruxolitinib on treating steroid-refractory acute GVHD has been verified by the REACH1/2 study; however, its safety and efficacy in patients with steroid-refractory chronic GVHD (SR-cGVHD) remain unclear. In this retrospective study, 70 patients received ruxolitinib as a salvage therapy for SR-cGVHD. Twenty-four weeks after ruxolitinib treatment, the overall response rate (ORR) was 74.3% (52/70), including 34 patients who achieved complete remission (CR) and 18 who achieved partial remission (PR). The main adverse event was cytopenia, which occurred in 51.4% (36/70) of patients. After ruxolitinib treatment, the percentage of CD4 cells increased from 18.20% to 23.22% (<0.001), while the percentages of NK (CD16CD56) cells and regulatory T cells (CD4CD127 CD25) decreased (<0.001, <0.001). Among the B cell subsets, the proportion of total B cells approximately tripled from 3.69% to 11.16% (<0.001). Moreover, we observed a significant increase in IL-10 levels after ruxolitinib treatment (=0.025) and a remarkable decrease in levels of suppression of tumorigenicity 2 (ST2) from 229.90 ng/ml to 72.65 ng/ml. The median follow-up after the initiation of ruxolitinib treatment was 401 (6-1076) days. The estimated one-year overall survival rate of the whole group was 66.0% (54.4-77.6%, 95% CI), and the one-year overall survival rate of patients with mild and moderate cGVHD was 69.6% (57.4-81.8%, 95% CI), which was better than that of patients with severe cGVHD (31.3%, 0.0-66.2%, 95% CI) (=0.002). Patients who achieved a CR and PR achieved better survival outcomes (84.5%, 73.9-95.1%, 95% CI) than those who showed NR to ruxolitinib treatments (16.7%, 0-34.3%, 95% CI) (<0.001). At the final follow-up, cGVHD relapse occurred in six patients after they reduced or continued their ruxolitinib doses. Collectively, our results suggest that ruxolitinib is potentially a safe and effective treatment for SR-cGVHD.
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http://dx.doi.org/10.3389/fimmu.2021.673636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278571PMC
June 2021

Enhancement of conductive drying of sewage sludge with mechanical compression: Drying kinetics, and interfacial heat transfer behavior.

Sci Total Environ 2021 Jun 27;796:148716. Epub 2021 Jun 27.

School of Environmental Science & Technology, Dalian University of Technology, Dalian 116024, Liaoning, China. Electronic address:

Improving sludge drying efficiency is of tremendous importance for public health, subsequent treatment, and comprehensive utilization. The interfacial thermal resistance between sludge and hot wall greatly limits the conductive drying performance. This study employed mechanical compression to decrease the interfacial thermal resistance. The drying kinetics and interfacial heat transfer behavior were investigated at mechanical loads of 25 to 200 kPa, temperatures of 120 to 210 °C, and sludge thicknesses of 1.0 to 3.0 mm, and were compared to those in the conventional drying process without mechanical load. The increase of temperature and mechanical load and the decrease of thickness improved drying rates. The drying experienced one warm-up period and two falling rate periods. The breakthrough of interfacial vapor film was responsible for the rapid rise in drying rates initially. At the thickness of 3.0 mm, 210 °C, and 100 kPa, the effective moisture diffusivity was increased by 2.5 times, and the apparent activation energy was reduced by 34% compared to the traditional process in the first falling rate period, implying that mechanical compression facilitated moisture migration and bound water desorption. The effective moisture diffusivity in the first falling rate period was increased by 35% compared to the diffusivity in the second falling rate period because of the pressure-driven flow. The decrease in drying rates was due to the transformation from the pressure-driven flow to vapor diffusion-limited flow in the first falling rate period. Additionally, this study provided essential information on developing a new sludge treatment method and establishing the drying model.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148716DOI Listing
June 2021

Effects of sodium humate and glutamine combined supplementation on growth performance, diarrhea incidence, blood parameters, and intestinal microflora of weaned calves.

Anim Sci J 2021 Dec;92(1):e13584

Heilongjiang Key Laboratory of Experimental Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

This experiment was conducted to investigate the effects of sodium humate (HNa) and glutamine (Gln) alone or combined supplementation on growth performance, diarrhea incidence, blood parameters, and intestinal microflora of weaned Holstein calves. In a 14-day experiment, 40 calves at 51 ± 3 days of age were randomly allocated to four treatment groups: (1) NC (basal diet), (2) NC + 5% HNa, (3) NC + 1% Gln, and (4) NC + 5% HNa + 1% Gln. Calves combined supplementation with HNa and Gln had a higher (P < .05) ADG, serum concentration of glucose (GLU), IgA, and IgG but lower fecal scores, diarrhea incidence, serum concentration of TNF-α, and IL-10 compared with NC group (P < .05). Compared with NC group, HNa + Gln group showed higher (P < .05) serum GSH and T-AOC activities but lower (P < .05) concentration of MDA and D-lac. Furthermore, the abundances of Prevotella ruminicola, Bifidobacterium, and Lactobacillus in rectal digesta were increased (P < .05), but the Escherichia coli was significantly decreased. In conclusion, combined supplementation with HNa and Gln can effectively improve the immune status, antioxidant capacity, and intestinal microflora of the weaned calves while reducing diarrhea incidence.
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http://dx.doi.org/10.1111/asj.13584DOI Listing
December 2021

Characteristics and prognosis of telangiectatic osteosarcoma: a population-based study using the Surveillance, Epidemiology and End Results (SEER) database.

Ann Transl Med 2021 May;9(9):796

Department of Orthopaedic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Telangiectatic osteosarcoma (TOS) is a rare type of osteosarcoma for which limited clinical data is available. Furthermore, the clinical characteristics and prognosis of TOS remain unclear.

Methods: A large population-based cohort analysis was conducted using the Surveillance, Epidemiology and End Results (SEER) registry. The data of TOS and conventional osteosarcoma (COS) patients from 2000 to 2017 were collected. The categorical variables were assessed by Chi-squared tests. Kaplan-Meier curves and log-rank (Mantel-Cox) tests were used to examine the survival outcomes between the groups. Cox proportional hazard models were used for univariate and multivariate analyses of TOS patient survival-related variables.

Results: A total of 141 TOS patients and 2961 COS patients were included in this analysis, and the mean age at diagnosis was 23.5 and 29.4 years, respectively. Compared to COS patients, TOS patients were more likely to be under 20 years old (61.7% 51.7%, P=0.022), and without a second peak of incidence after 60 years of age. The median overall survival (mOS) of TOS patients was not reached compared to a median survival of 84 months for COS patients (hazard ratio 0.75, 95% confidence interval 0.59 to 0.95, P=0.0175). After adjusting these data for age at diagnosis, stage, and surgery at the primary site, no significant differences in mOS were observed between the two groups. In univariate analyses, being under 20 years of age, having localized or regional stage disease, and having undergone surgery were associated with a decreased risk of death. Subsequent multivariate analysis indicated that age at diagnosis, stage, and surgery at the primary site were all independent predictors of prognosis in TOS patients.

Conclusions: Patients with TOS were younger than patients with COS and did not show a second peak after 60 years of age. Age, summary stage at diagnosis, and surgery at the primary site were independent predictors of survival for TOS patients.
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http://dx.doi.org/10.21037/atm-20-8001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246211PMC
May 2021

The effect of atrial fibrillation on perioperative outcomes in patients with pancreatic cancer undergoing open pancreaticoduodenectomy: analysis of the National Inpatient Sample.

Gland Surg 2021 Jun;10(6):1951-1961

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University; Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing, China.

Background: To assess the role of atrial fibrillation on perioperative outcomes in patients with pancreatic cancer undergoing open pancreaticoduodenectomy (OPD).

Methods: We investigated patients with pancreatic cancer undergoing OPD during 2012-2014 within National Inpatient Sample database. The study population was divided into two groups based on the presence of atrial fibrillation. In-hospital mortality, length of stay, cost of hospitalization, and in-hospital complications were compared between the two groups. Logistic regression models and linear regression were used to adjust for potential confounders. Propensity score matching was also utilized.

Results: Of the 12,785 patients aged ≥18 years undergoing OPD during years 2012-2014, 11,469 (90%) had no atrial fibrillation and 1,316 (10%) had atrial fibrillation. Patients with atrial fibrillation were found to have significantly higher cost, but similar mortality and LOS compared to those without atrial fibrillation. The risk of gastrointestinal anastomotic leakage, cardiac complications, respiratory complications, pulmonary embolism, and perioperative shock were found to be significantly higher in atrial fibrillation group than non-atrial fibrillation group in both multivariate regression model and propensity score matching model. In older patients (>65 years), atrial fibrillation was found to be associated with a significantly higher cost, longer hospital stays, higher incidence of cardiac complications, respiratory complications, and postoperative shock, yet similar mortality.

Conclusions: Atrial fibrillation was found to be associated with higher cost in pancreatic cancer patients undergoing OPD, as well as increased occurrence of cardiac complications, respiratory complications, pulmonary embolism, and perioperative shock. Surgeons should pay special attention to patients with atrial fibrillation, and consider working together with cardiologists and anesthesiologists to jointly develop a management plan.
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http://dx.doi.org/10.21037/gs-21-116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258882PMC
June 2021

Embryonic Stem Cell-Derived Exosomes Attenuate Transverse Aortic Constriction Induced Heart Failure by Increasing Angiogenesis.

Front Cardiovasc Med 2021 28;8:638771. Epub 2021 Jun 28.

Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Although there are concerns regarding their clinical use, embryonic stem cells (ESCs) hold a great promise for cardiac repair. Exosomes deriving from ESCs constitute a promising alternative for heart restoration. However, their effects in hypertension-induced heart failure are still unknown. To investigate the effects of ESCs-derived exosomes on hypertension-induced heart failure and the underlying mechanisms, sustained transverse aortic constriction (TAC) was performed on 8-week-old C57BL/6 male mice. After 1 months, ESCs-derived exosomes were isolated and injected intravenously once a week for 6 weeks. Echocardiography, wheat germ agglutinin (WGA), Masson staining, immunohistochemistry, and tube formation assays were all involved in our study. Proteomics analyses revealed that ESC-derived exosomes contain FGF2 protein. Tube formation induced by these exosomes could be inhibited by FGF2R siRNA interference. ESCs-derived exosomes evidently attenuated TAC-induced heart failure, improving cardiac function and promoting myocardial angiogenesis which can be attenuated by selective FGF2 inhibitor AZD4547. ESC-derived exosomes attenuate TAC-induced heart failure mostly by promoting myocardial angiogenesis. FGF2 signaling plays a vital role in the myocardial angiogenesis induced by ESC-derived exosomes.
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http://dx.doi.org/10.3389/fcvm.2021.638771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273241PMC
June 2021

Ten-gene signature reveals the significance of clinical prognosis and immuno-correlation of osteosarcoma and study on novel skeleton inhibitors regarding MMP9.

Cancer Cell Int 2021 Jul 14;21(1):377. Epub 2021 Jul 14.

Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China.

Objectives: This study aimed to identify novel targets in the carcinogenesis, therapy and prognosis of osteosarcoma from genomic level, together with screening ideal lead compounds with potential inhibition regarding MMP-9.

Methods: Gene expression profiles from GSE12865, GSE14359, GSE33382, GSE36001 and GSE99671 were obtained respectively from GEO database. Differentially expressed genes were identified, and functional enrichment analysis, such as GO, KEGG, GSEA, PPI were performed to make a comprehensive understanding of the hub genes. Next, a series of high-precision computational techniques were conducted to screen potential lead compounds targeting MMP9, including virtual screening, ADME, toxicity prediction, and accurate docking analysis.

Results: 10 genes, MMP9, CD74, SPP1, CXCL12, TYROBP, FCER1G, HCLS1, ARHGDIB, LAPTM5 and IGF1R were identified as hub genes in the initiation of osteosarcoma. Machine learning, multivariate Cox analysis, ssGSEA and survival analysis demonstrated that these genes had values in prognosis, immune-correlation and targeted treatment. Tow novel compounds, ZINC000072131515 and ZINC000004228235, were screened as potential inhibitor regarding MMP9, and they could bind to MMP9 with favorable interaction energy and high binding affinity. Meanwhile, they were precited to be efficient and safe drugs with low-ames mutagenicity, none weight evidence of carcinogenicity, as well as non-toxic with liver.

Conclusions: This study revealed the significance of 10-gene signature in the development of osteosarcoma. Besides, drug candidates identified in this study provided a solid basis on MMP9 inhibitors' development.
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http://dx.doi.org/10.1186/s12935-021-02041-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281696PMC
July 2021

Clinical Features and Prognostic Factors of Children With Chronic Active Epstein-Barr Virus Infection: A Retrospective Analysis of a Single-Center.

J Pediatr 2021 Jul 11. Epub 2021 Jul 11.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology; National Key Discipline of Pediatrics (Capital Medical University); Key Laboratory of Major Disease in Children, Ministry of Education; Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, China, 100045.

Objective: To analyze the clinical characteristics, prognosis factors and risk factors of chronic active Epstein-Barr virus (EBV) infection in children.

Study Design: Observational analysis of baseline data and follow-up evaluation data of children with chronic active EBV infection in our center between January 1, 2016 and December 31, 2019, and were followed through June 30, 2020.

Results: There were 96 children with chronic active EBV infection, including 50 males and 46 females, with the median age of 6.7 years (range from 0.6-17.6 years) at diagnosis. The median follow-up time was 16.5 months. The three most common clinical manifestations were fever, lymph node enlargement, and hepatomegaly or splenomegaly. Thirty-three patients (36.3%) also had a diagnosis of hemophagocytic lymphohistiocytosis (HLH). EBV infected only T lymphocytes, NK cells, or both T- and NK-cell types in 15 (33.3%), 17 (37.8%), and 13 (28.9%), respectively. At the end of follow up, 26 children had died and 60 survived, 10 were lost to follow up. Generally, progression-free survival was 69.8% ± 2.4%. The level of 'IL-6 and IL-10' and the combination of 'younger age and lower pathologic grade' at diagnosis were independent prognostic factors by Cox regression analysis (P = .009 and 0.018, respectively).

Conclusions: Children with lower levels of IL-6 and IL-10, or with younger age and lower pathologic grades, generally had favorable outcomes at the terminal point of follow up, indicating better prognostic signs.
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http://dx.doi.org/10.1016/j.jpeds.2021.07.009DOI Listing
July 2021

Bioinformatics Technologies in Autophagy Research.

Adv Exp Med Biol 2021 ;1208:387-453

Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Autophagy is an important and dynamic biological process, and provides an ideal application scenario for bioinformatics to develop new data resources, algorithms, tools and computational or mathematic models for a better understanding of complex regulatory mechanisms in cells. In the past decade, great efforts have been taken on the development of numerous bioinformatics technologies in autophagy research, and a comprehensive summarization of these important studies will provide a timely reference for both biologists and bioinformaticians who are working in the field of autophagy. In this book chapter, we first introduce bioinformatics technologies that allow sequence analysis of autophagy genes. We briefly summarize the mainstream algorithms in sequence alignment for the identification of homologous autophagy genes and emphasize the computational identification of potential orthologs and paralogs, as well as the evolutionary analysis of autophagy gene families. Three methods for the recognition of autophagy-related sequence motifs are introduced: regular expression, position-specific scoring matrix (PSSM) and group-based prediction system (GPS). Second, we carefully summarize recent progress in the analysis of autophagy-related omics data. We discuss how two major types of computational methods, enrichment analysis and network analysis can be used to analyze omics data, including transcriptomics, non-coding RNAomics, epigenomics, proteomics, phosphoproteomics and protein lysine modification (PLM) omics data. Finally, we summarize several important autophagy-related data resources, including both autophagy gene databases and autophagy-related RNA databases. We anticipate that more useful bioinformatics technologies will be developed and play an ever-more-important role in the analysis of autophagy.
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http://dx.doi.org/10.1007/978-981-16-2830-6_18DOI Listing
July 2021
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