Publications by authors named "Dong Wang"

4,429 Publications

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Enhanced effect of combining bone marrow mesenchymal stem cells (BMMSCs) and pulsed electromagnetic fields (PEMF) to promote recovery after spinal cord injury in mice.

MedComm (2020) 2022 Sep 3;3(3):e160. Epub 2022 Aug 3.

Rehabilitation Medicine Center and Institute of Rehabilitation Medicine, West China Hospital Sichuan University Chengdu PR China.

Spinal cord injury (SCI) is a destructive traumatic disease of the central nervous system without satisfying therapy efficiency. Bone marrow mesenchymal stem cells (BMMSCs) therapy promotes the neurotrophic factors' secretion and axonal regeneration, thereby promoting recovery of SCI. Pulsed electromagnetic fields (PEMF) therapy has been proven to promote neural growth and regeneration. Both BMMSCs and PEMF have shown curative effects for SCI; PEMF can further promote stem cell differentiation. Thus, we explored the combined effects of BMMSCs and PEMF and the potential interaction between these two therapies in SCI. Compared with the SCI control, BMMSCs, and PEMF groups, the combinational therapy displayed the best therapeutic effect. Combinational therapy increased the expression levels of nutritional factors including brain-derived neurotrophic factor (BDNF), nerve growth factors (NGF) and vascular endothelial growth factor (VEGF), enhanced neuron preservation (NeuN and NF-200), and increased axonal growth (MBP and myelin sheath). Additionally, PEMF promoted the expression levels of BDNF and VEGF in BMMSCs via Wnt/β-catenin signaling pathway. In summary, the combined therapy of BMMSCs and PEMF displayed a more satisfactory effect than BMMSCs and PEMF therapy alone, indicating a promising application of combined therapy for the therapy of SCI.
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http://dx.doi.org/10.1002/mco2.160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350428PMC
September 2022

Serum complement C1q level is associated with left ventricular hypertrophy induced by coarctation of the aorta: A retrospective observational study.

BMC Cardiovasc Disord 2022 Aug 10;22(1):367. Epub 2022 Aug 10.

Beijing Institute of Heart, Lung and Blood Vessel Diseases, Capital Medical University, No.2 Anzhen Street, Chaoyang District, Beijing, 100029, China.

Background: The complement system plays an important role in the development of left ventricular hypertrophy. Complement C1q is an initial component of the classical complement pathway and is related to many inflammatory diseases. We aimed to determine whether there was an association between serum complement C1q and left ventricular hypertrophy induced by coarctation of the aorta (CoA).

Methods: Based on whether CoA was combined with a large ventricular septal defect (VSD) or patent ductus arteriosus (PDA), the patients were divided into a simple CoA group (n = 15) and a complex CoA group (n = 13). Meanwhile, we selected simple large VSD (n = 14) patients and normal children (n = 28) as the control group. The serum complement C1q level was compared using immunity transmission turbidity among different groups.

Results: The preoperative content of C1q in the simple CoA group was significantly lower than that in the complex CoA group and normal group (96.97 ± 20.66 vs. 130.73 ± 35.78, 96.97 ± 20.66 vs. 156.21 ± 29.14, P < 0.05). There was no significant difference in the preoperative content of C1q between the complex CoA group and the large VSD group (P > 0.05). There was a negative correlation between the preoperative complement C1q content and the interventricular septal thickness and left ventricular posterior wall thickness (r = - 0.035, r = - 0.288, P < 0.05). The percentage of postoperative decrease in C1q in children with simple CoA or complex CoA was positively correlated with the time of cardiopulmonary bypass and aortic cross clamp, respectively (r = 0.797, r = 0.622, r = 0.898, r = 0.920, P < 0.05). There was no significant difference in the content of preoperative triglycerides (TG), total cholesterol (TCHO), high-density lipoprotein cholesterol (HDL-C) or low-density lipoprotein cholesterol (LDL-C) among the different groups (P > 0.05). In the simple CoA group and complex CoA group, the preoperative complement C1q, TG, TCHO, HDL-C and LDL-C levels were significantly higher than those after the operation (P < 0.05). There was no significant correlation between preoperative complement C1q and TG, TCHO, HDL-C or LDL-C (P > 0.05).

Conclusions: Complement C1q has an inhibitory effect on the formation of left ventricular hypertrophy, which may not be mediated by regulating lipid metabolism. During cardiac surgery, complement C1q may have a protective effect against myocardial injury.
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http://dx.doi.org/10.1186/s12872-022-02807-2DOI Listing
August 2022

Inspiration from nature: BioAIEgens for biomedical and sensing applications.

Biomaterials 2022 Aug 5:121712. Epub 2022 Aug 5.

Centre for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Material Science and Engineering, Shenzhen University, Shenzhen 518061, China; School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, China. Electronic address:

Owing to high sensitivity, selectivity, and non-invasiveness, fluorescence has been widely applied in the biomedical and sensing fields. Among the pool of fluorescent probes, luminogens with aggregation-induced emission (AIE) characteristic exhibit unique strengths in biological applications. However, most reported AIE luminogens (AIEgens) require complicated synthetic procedures, which raise the costs and biocompatibility concerns, especially in biomedical imaging and therapy. In contrast, bioproduct-inspired AIEgens (BioAIEgens) can compensate for the weakness of synthetic AIEgens in terms of their high biocompatibility, low costs, and easy preparation. This review highlights the latest development of BioAIEgens discovered from natural herbs, as well as their potential biomedical and sensing applications. As nature is full of potential resources, studying AIEgens from natural herbs can facilitate the strength of AIE properties in diverse applications and offer more inspiration to the future BioAIEgen structural design and development.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121712DOI Listing
August 2022

Downregulation of miR-20b-5p Contributes to the Progression of Liver Fibrosis via the STAT3 Signaling Pathway In Vivo and In Vitro.

Dig Dis Sci 2022 Aug 10. Epub 2022 Aug 10.

Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

Background: Activated hepatic stellate cells (HSCs) are primarily involved in liver fibrosis and portal hypertension (PHT). We aimed to investigate the effect of miR-20b-5p on HSCs, liver fibrosis, and PHT.

Methods: MiR-20b-5p expression in HSCs and in mouse liver fibrosis was determined by qPCR. Further, the effects of miR-20b-5p mimic on HSCs migration, proliferation, and apoptosis were investigated in vitro. A dual-luciferase reporter assay was performed to confirm the direct interaction between miR-20b-5p and STAT3. In vivo, mouse liver fibrosis was established by common bile duct ligation and intervened by agomiR-20b-5p. Sirius red staining and hydroxyproline content were used to evaluate collagen deposition. The α-SMA expression in the liver was detected by IHC and Western blotting. The STAT3 signaling pathway and its downregulated cytokines as well as portal pressure and angiogenesis were explored.

Results: MiR-20b-5p was significantly downregulated during HSCs activation and in mouse liver fibrosis. The functional analyses demonstrated that miR-20b-5p inhibited cell proliferation, activation, and promoted apoptosis in HSCs in vitro. Moreover, miR-20b-5p regulated STAT3 expression by binding to the 3'UTR of its miRNA directly. Overexpression of miR-20b-5p facilitated HSC activation and proliferation by inhibiting the STAT3 signaling pathway. MiR-20b-5p overexpression suppressed the STAT3 and its downstream cytokines and ameliorated liver fibrosis in mice. The intra- and inter-hepatic angiogenesis were also effectively inhibited. The inhibition of liver fibrosis and neoangiogenesis contributed to the decrease of portal pressure.

Conclusions: MiR-20b-5p plays an important role in the fibrosis and angiogenesis of liver fibrosis by targeting the STAT3 signaling pathway.
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http://dx.doi.org/10.1007/s10620-022-07660-zDOI Listing
August 2022

Water quality status response to multiple anthropogenic activities in urban river.

Environ Sci Pollut Res Int 2022 Aug 10. Epub 2022 Aug 10.

School of Geographical Information and Tourism, Chuzhou University, Chuzhou, 239000, China.

Water quality evaluation and degrading factors identification are crucial for predicting water quality evolution trends in an urban river. However, under the coupling of multiple factors, these targets face great challenges. The water quality status response to multiple anthropogenic activities in an urban river was evaluated and predicted based on comprehensive assessment methods and random forest (RF) model. We found that the distribution of each physicochemical parameter exhibits an obvious spatial clustering. The mean pollution level and trophic status of the urban river are medium pollution (water quality index = 59.79; Nemerow's pollution index = 2.00) and light eutrophication (trophic level index = 57.30). The water quality status is sensitive to anthropogenic activities, showing the following order of TLI and NPI values: residential district > industrial district > agricultural district and downtown > suburbs > countryside. According to the redundancy analysis, constructed land (F = 15.90, p < 0.01) and domestic sewage (F = 14.20, p < 0.01) evinced as the crucial factors that aggravated the water quality pollution level. Based on the simulation results of the RF model (variation explained = 94.91%; R = 0.978), improving domestic sewage treatment standards is the most effective measure to improve the water quality (increased by 40.3-49.3%) in residential and industrial districts. While in a suburban district, improving the domestic sewage collection rate has more effectively (23%) than those in the residential and industrial districts. Conclusively, reducing exogenous pollution input and improving domestic sewage treatment standards are vital to urban river restoration. Clinical trial registration Not applicable.
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http://dx.doi.org/10.1007/s11356-022-22378-1DOI Listing
August 2022

Nested order-disorder framework containing a crystalline matrix with self-filled amorphous-like innards.

Nat Commun 2022 Aug 9;13(1):4650. Epub 2022 Aug 9.

Center for High Pressure Science and Technology Advanced Research, Shanghai, 201203, China.

Solids can be generally categorized by their structures into crystalline and amorphous states with different interactions among atoms dictating their properties. Crystalline-amorphous hybrid structures, combining the advantages of both ordered and disordered components, present a promising opportunity to design materials with emergent collective properties. Hybridization of crystalline and amorphous structures at the sublattice level with long-range periodicity has been rarely observed. Here, we report a nested order-disorder framework (NOF) constructed by a crystalline matrix with self-filled amorphous-like innards that is obtained by using pressure to regulate the bonding hierarchy of CuSbS. Combined in situ experimental and computational methods demonstrate the formation of disordered Cu sublattice which is embedded in the retained crystalline Cu framework. Such a NOF structure gives a low thermal conductivity (~0.24 W·m·K) and a metallic electrical conductivity (8 × 10 Ω·m), realizing the collaborative improvement of two competing physical properties. These findings demonstrate a category of solid-state materials to link the crystalline and amorphous forms in the sublattice-scale, which will exhibit extraordinary properties.
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http://dx.doi.org/10.1038/s41467-022-32419-5DOI Listing
August 2022

Mechanism of Undergraduate Students' Waste Separation Behavior in the Environmentally Friendly Higher Education Mega Center of Guangzhou.

J Environ Public Health 2022 30;2022:4475245. Epub 2022 Jul 30.

School of Management, Shenzhen University, Shenzhen 518060, China.

The volume of waste produced by aspects of both industrial life and daily life has increased in the past few years in conjunction with the rapidly growing economy in China. In urban areas, citizens consume more resources and produce much waste, which pollutes the environment. Many cities have proposed numerous regulations for waste separation to help build environmentally friendly cities. Plenty of studies tried to reveal the mechanism of residents' waste separation behavior in different theoretical perspectives but were unable to clarify the effect of waste separation factors in university. Moreover, the response and performance of undergraduate students after the waste separation regulations are proposed and are not well discussed. To clarify the mechanism of waste separation behavior in campus and evaluate the response and performance, in this study, a questionnaire is used to sample undergraduate students from the Higher Education Mega Center in Guangzhou. With the use of SPSS 23.0, this study conducts an empirical analysis; the results of which show that (1) environmental awareness and personal responsibility have a significant positive impact on behavior attitude; (2) convenience and economic cost have a significant positive impact on perceived behavior control; (3) intention has a partial mediating role between behavior attitude, subjective norms, and perceived behavior control; and (4) habits directly affect behavior and regulate the relationship between intention and behavior. The results will provide some meaningful implications for the government.
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http://dx.doi.org/10.1155/2022/4475245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356847PMC
August 2022

Effects of dietary protein levels on production performance, meat quality and flavor of fattening pigs.

Front Nutr 2022 22;9:910519. Epub 2022 Jul 22.

Gansu Longyuan Agricultural Economic Cooperation Center, Lanzhou, China.

This study aimed to evaluate the effects of dietary protein level on the production performance, slaughter performance, meat quality, and flavor of finishing pigs. Twenty-seven Duroc♂ × Bamei♀ binary cross-bred pigs (60.86 ± 2.52 kg body weight) were randomly assigned to three groups, each group has three replicates, and each replicate has three pigs. Three groups of finishing pigs were fed 16.0, 14.0, and 12.0% crude protein levels diets, and these low-protein diets were supplemented with four limiting amino acids (lysine, methionine, threonine and tryptophan). The results showed that the pigs fed low-protein diets increased < 0.05) loin eye muscle area, and reduced ( < 0.05) heart weight, lung weight. The feed-weight ratio of the 14.0% protein group was reduced ( > 0.05); Dietary protein levels significantly affected the luminance (L), yellowness (b and b) ( < 0.05), reduced shear stress, muscle water loss, drip loss, the levels of crude fat ( < 0.05), and increased marbling score ( < 0.05) in the muscle of finishing pigs; The low-protein diets improved PUFA/TFA, PUFA/SFA ( > 0.05), and increased hexanal, E-2-heptenal, 1-octen-3-ol, EAA/TAA in the muscle of finishing pigs ( < 0.05); The results indicated that reduced the crude protein levels of dietary by 2.0-4.0%, and supplementation with four balanced limiting amino acids had no significant effects on the production performance and slaughter performance of finishing pigs, and could effectively improve meat quality and flavor.
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http://dx.doi.org/10.3389/fnut.2022.910519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354234PMC
July 2022

Establishment and validation of a prediction model for self-absorption probability of chronic subdural hematoma.

Front Neurol 2022 22;13:913495. Epub 2022 Jul 22.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.

Background: Chronic subdural hematoma (CSDH) is common in elderly people with a clear or occult traumatic brain injury history. Surgery is a traditional method to remove the hematomas, but it carries a significant risk of recurrence and poor outcomes. Non-surgical treatment has been recently considered effective and safe for some patients with CSDH. However, it is a challenge to speculate which part of patients could obtain benefits from non-surgical treatment.

Objective: To establish and validate a new prediction model of self-absorption probability with chronic subdural hematoma.

Method: The prediction model was established based on the data from a randomized clinical trial, which enrolled 196 patients with CSDH from February 2014 to November 2015. The following subjects were extracted: demographic characteristics, medical history, hematoma characters in imaging at admission, and clinical assessments. The outcome was self-absorption at the 8th week after admission. A least absolute shrinkage and selection operator (LASSO) regression model was implemented for data dimensionality reduction and feature selection. Multivariable logistic regression was adopted to establish the model, while the experimental results were presented by nomogram. Discrimination, calibration, and clinical usefulness were used to evaluate the performance of the nomogram. A total of 60 consecutive patients were involved in the external validation, which enrolled in a proof-of-concept clinical trial from July 2014 to December 2018.

Results: Diabetes mellitus history, hematoma volume at admission, presence of basal ganglia suppression, presence of septate hematoma, and usage of atorvastatin were the strongest predictors of self-absorption. The model had good discrimination [area under the curve (AUC), 0.713 (95% , 0.637-0.788)] and good calibration ( = 0.986). The nomogram in the validation cohort still had good discrimination [AUC, 0.709 (95% , 0.574-0.844)] and good calibration ( = 0.441). A decision curve analysis proved that the nomogram was clinically effective.

Conclusions: This prediction model can be used to obtain self-absorption probability in patients with CSDH, assisting in guiding the choice of therapy, whether they undergo non-surgical treatment or surgery.
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http://dx.doi.org/10.3389/fneur.2022.913495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355276PMC
July 2022

Predictive Indicators for Complications of Proximal Humerus Fractures Treated with Locking Plate or Intramedullary Nail Fixation.

Orthop Surg 2022 Aug 4. Epub 2022 Aug 4.

Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, P.R. China.

Objective: The purpose of this study was to evaluate the best placement of calcar screws in proximal humerus fracture surgeries.

Methods: This retrospective cohort study included clinical and radiographic outcomes of 98 patients treated with proximal humerus fracture surgeries between January 2017 and June 2020. Demographic data of patients were obtained from medical records. The surgical and radiographic results were also collected: operation time, blood loss, time to surgery, fibular allograft, disruption of medial region hinge, Neer classification, and recovery of medial support. Patients were allocated into two groups: the locking plate group (n = 65) and the intramedullary nail group (n = 33). In this study, we proposed new predictive indicators, named horizontal ratio (HR) and vertical ratio (VR), to quantify the placement of calcar screws in these two groups. A receiver operating characteristic (ROC) analysis was conducted to display the accuracy of these indicators. Shoulder activity, visual scale analog (VAS) score, and Constant score were performed to evaluate postoperative clinical outcomes at 1 year follow-up.

Results: In the multivariate logistic regression analysis, only time to surgery and effective medial support were considered statistically significant factors of postoperative complications (p < 0.05). Significant differences were observed between medial support and postoperative complications both in the locking plate group and the intramedullary nail group (p < 0.05). Only the vertical ratio of locking plate (VRLP) was a statistically significant predictor of postoperative complications (p < 0.05). The area under curve was calculated to assess the predictive value of VRLP, which came to 0.84. In addition, a ROC analysis found quantifiable thresholds of the VR was 0.1713 as measures to avoid postoperative complications in the locking plate fixation.

Conclusion: In locking plate fixation, the incidence of postoperative complications increased significantly when the VR of calcar screws greater than 0.1713, which was beneficial to surgeons to place calcar screws.
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http://dx.doi.org/10.1111/os.13421DOI Listing
August 2022

Targeting clock-controlled gene Nrf2 ameliorates inflammation-induced intervertebral disc degeneration.

Arthritis Res Ther 2022 08 3;24(1):181. Epub 2022 Aug 3.

Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China.

Background: Intervertebral disc (IVD) is a highly rhythmic tissue, which experiences a diurnal cycle of high/low mechanical loading via the changes of activity/rest phase. There are signs that disruption of the peripheral IVD clock is related to the process of intervertebral disc degeneration (IDD). However, it is still unclear whether inflammation could disturb the IVD clock and thus induce the process of IDD.

Methods And Results: In this study, we used IL-1β, a commonly used inflammatory factor, to induce IDD and found that the IVD clock was dampened in degenerated human nucleus pulposus specimens, rat nucleus pulposus (NP) tissues, and cells. In this study, we found that the circadian clock of NP cells was totally disrupted by knockdown of the core clock gene brain and muscle arnt-like protein-1 (Bmal1), which thus induced the dysfunction of NP cells. Next, we explored the mechanism of dampened clock-induced IDD and found that knockdown of Bmal1 decreased the expression of nuclear factor erythroid2-related factor 2 (NRF2), a downstream target gene of Bmal1, and increased inflammatory response, oxidative stress reaction, and apoptosis of NP cells. In addition, NRF2 activation attenuated the dysfunction of NP cells induced by the dampened IVD clock and the degenerative process of NP tissues in an organotypic tissue-explant model.

Conclusions: Taken together, our study extends the relationship between peripheral clock and IVD homeostasis and provides a potential therapeutic method for the prevention and recovery of IDD by targeting the clock-controlled gene Nrf2.
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http://dx.doi.org/10.1186/s13075-022-02876-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347076PMC
August 2022

Variational methods and deep Ritz method for active elastic solids.

Soft Matter 2022 Aug 3. Epub 2022 Aug 3.

Physics Program, Guangdong Technion - Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong, 515063, China.

Variational methods have been widely used in soft matter physics for both static and dynamic problems. These methods are mostly based on two variational principles: the variational principle of minimum free energy (MFEVP) and Onsager's variational principle (OVP). Our interests lie in the applications of these variational methods to active matter physics. In our former work [H. Wang, T. Qian and X. Xu, , 2021, , 3634-3653], we have explored the applications of OVP-based variational methods for the modeling of active matter dynamics. In the present work, we explore variational (or energy) methods that are based on MFEVP for static problems in active elastic solids. We show that MFEVP can be used not only to derive equilibrium equations, but also to develop approximate solution methods, such as the Ritz method, for active solid statics. Moreover, the power of the Ritz-type method can be further enhanced using deep learning methods if we use deep neural networks to construct the trial functions of the variational problems. We then apply these variational methods and the deep Ritz method to study the spontaneous bending and contraction of a thin active circular plate that is induced by internal asymmetric active contraction. The circular plate is found to be bent towards its contracting side. The study of such a simple toy system gives implications for understanding the morphogenesis of solid-like confluent cell monolayers. In addition, we introduce a so-called activogravity length to characterize the importance of gravitational forces relative to internal active contraction in driving the bending of the active plate. When the lateral plate dimension is larger than the activogravity length (about 100 micron), gravitational forces become important. Such gravitaxis behaviors at multicellular scales may play significant roles in the morphogenesis and in the up-down symmetry broken during tissue development.
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http://dx.doi.org/10.1039/d2sm00404fDOI Listing
August 2022

New seco-anthraquinone glucoside from the roots of Rumex crispus.

Nat Prod Bioprospect 2022 Aug 3;12(1):29. Epub 2022 Aug 3.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China.

A new seco-anthraquinone, crispuside A (1), and three new 3,4-dihydronaphthalen-1(2H)-ones, napthalenones A-C (2-4), were isolated from the roots of Rumex crispus L., along with 10 known anthraquinones (6-14) and naphthalenone (5). Their structures were fully determined by extensive spectroscopic analyses, including ECD, and X-ray crystallography in case of compound 5, whose absolute configuration was determined for the first time. The isolates 1, 6-14 were evaluated for their anti-inflammatory and anti-fungal activity against three skin fungi, e.g., Epidermophyton floccosum, Trichophyton rubrum, and Microsporum gypseum. Most of the isolates showed weak anti-fungal and anti-inflammatory activity. Only compound 9 exhibited obvious anti-fungal activity against E. floccosum (MIC = 2.467 ± 0.03 μM) and M. gypseum (MIC = 4.673 ± 0.077 μM), while the MIC values of the positive control terbinafine were 1.287 ± 0.012 and 0.077 ± 0.00258 μM, respectively. The results indicated that simple emodin type anthraquinone is more potential against skin fungi than its oxyglucosyl, C-glucosyl and glycosylated seco analogues.
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http://dx.doi.org/10.1007/s13659-022-00350-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346041PMC
August 2022

Boosting Protein-Ligand Binding Pose Prediction and Virtual Screening Based on Residue-Atom Distance Likelihood Potential and Graph Transformer.

J Med Chem 2022 Aug 2;65(15):10691-10706. Epub 2022 Aug 2.

Innovation Institute for Artificial Intelligence in Medicine of Zhejiang University, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.

The past few years have witnessed enormous progress toward applying machine learning approaches to the development of protein-ligand scoring functions. However, the robust performance and wide applicability of scoring functions remain a big challenge for increasing the success rate of docking-based virtual screening. Herein, a novel scoring function named RTMScore was developed by introducing a tailored residue-based graph representation strategy and several graph transformer layers for the learning of protein and ligand representations, followed by a mixture density network to obtain residue-atom distance likelihood potential. Our approach was resolutely validated on the CASF-2016 benchmark, and the results indicate that RTMScore can outperform almost all of the other state-of-the-art methods in terms of both the docking and screening powers. Further evaluation confirms the robustness of our approach that can not only retain its docking power on cross-docked poses but also achieve improved performance as a rescoring tool in larger-scale virtual screening.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00991DOI Listing
August 2022

Neoadjuvant Chemotherapy Improves the Immunosuppressive Microenvironment of Bladder Cancer and Increases the Sensitivity to Immune Checkpoint Blockade.

J Immunol Res 2022 21;2022:9962397. Epub 2022 Jul 21.

Department of Oncology, Army Medical Center, Chongqing 400042, China.

Although tumor immune microenvironment plays an important role in antitumor therapy, few studies explored the gene signatures associated with the tumor immune microenvironment of bladder cancer after neoadjuvant chemotherapy. We examined and analyzed differentially expressed genes from 9 patients with stage I-III bladder cancer by RNA immune-oncology profiling platform. After neoadjuvant chemotherapy, the expressions of 43 genes in 19 pathways and 10 genes in 5 pathways were upregulated and downregulated, respectively. Neoadjuvant chemotherapy also promoted the expression of genes related to the activation of antitumor immune responses and decreased the expression of genes related to tumor proliferation pathways. In addition, neoadjuvant chemotherapy improved tumor response to immune checkpoint blockade. Furthermore, this study also identified several genes that can be used to predict the efficacy of neoadjuvant chemotherapy and their possible molecular mechanisms. In conclusion, neoadjuvant chemotherapy may promote the activation of antitumor effects, improve the suppressive tumor immune microenvironment, and increase the sensitivity of bladder cancer to immune checkpoint blockade.
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http://dx.doi.org/10.1155/2022/9962397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338739PMC
August 2022

Aromatic Ring Fusion to Benzoporphyrin γ- Cyclodehydrogenation on a Ag(111) Surface.

ACS Nano 2022 Aug 1. Epub 2022 Aug 1.

Key Laboratory of Molecular Nanostructure and Nanotechnology and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, People's Republic of China.

Aromatic ring fusion to porphyrins and their derivatives represents an attractive route to tune the molecular conjugation and thus expand their functionalities. Here, we report the expansion of the aromatic π-system of palladium tetraphenyltetrabenzoporphyrins (Pd-TPTBP) surface-assisted γ- cyclodehydrogenation on Ag(111). The chemical transformation of Pd-TPTBP into different products at an elevated temperature of 600 K was revealed at the single-molecule level using bond-resolved scanning tunneling microscopy with a CO-functionalized tip. We captured a series of γ cyclodehydrogenation products, wherein the maximum extent to which the reaction can progress is associated with 7-fold C-C formation to afford nearly planar γ- fused porphyrins with 66 conjugated π-electrons. In addition, a small number of molecules undergo C-C bond dissociation of -phenyl at elevated temperature, producing fully planar γ- fused products lacking one or two phenyl moieties. Scanning tunneling spectroscopy measurements and DFT calculations suggest the electronic gap of the γ fused porphyrin decreases compared to that of the precursor. The HOMO and LUMO of the planar γ- fused products are localized on the partially fused benzo moieties and the -position, respectively.
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http://dx.doi.org/10.1021/acsnano.2c05819DOI Listing
August 2022

Effect of Tranexamic Acid on Blood Management during a High Tibial Osteotomy: A Systematic Review and Meta-analysis.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

West China Hospital, Sichuan University, Chengdu, China.

This study aimed to evaluate the efficiency and safety of tranexamic acid for blood management during high tibial osteotomy (HTO). A systematic search was conducted in Medline, Embase, and the Cochrane library database. Six studies and 208 patients were included in this meta-analysis using Review Manager V.5.3 and Stata 15.1 software. For primary outcomes, tranexamic acid lowered the total blood loss (WMD = -219.47, 95% CI [-355.61, -83.33], P = 0.002). For secondary outcomes, a significant reduction was found for decreased hemoglobin (POD1: WMD = -9.86, 95% CI [-13.45, -6.28], P < 0.05; POD2: WMD = -8.41, 95% CI [-11.50, -5.32], P < 0.05; POD5: WMD = -11.48, 95% CI [-14.56, -8.39], P < 0.05) and drainage (total: WMD = -105.93, 95% CI [-187.08, -24.78], P < 0.05; POD1: WMD = -122.195, 95% CI [-168.902, -75.488], P < 0.05). The sex difference (male/female ratio) was determined (total blood loss: P = 0.025; total drainage amount: p = 0.018) using meta-regression analysis. Females benefited more from tranexamic acid in terms of total blood loss (M/F > 40%: WMD = -53.11, 95% CI [-100.16, -6.05], P = 0.03; 40% ≥ M/F ≥ 20%: WMD = -362.20, 95% CI [-423.96, -300.45], P < 0.05; M/F < 20%: WMD = -263.00, 95% CI [-277.17, -248.83], P < 0.05) and total drainage (M/F > 40%: WMD = -7.11, 95% CI [-10.75, -3.47], P < 0.05; 40% ≥ M/F ≥ 20%: WMD = -104.72, 95% CI [-155.36, -54.08], P < 0.05; M/F < 20%: WMD = -222.00, 95% CI [-297.42, -146.58], P < 0.05). No significant differences were found for drainage on POD2 and POD3, wound complications, orthromboembolic events. In conclusion, tranexamic acid is effective and safe for blood management during HTO. Females appeared to benefit more from it, and an additional postoperative dose is suggested fora better effect.
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http://dx.doi.org/10.1111/os.13407DOI Listing
August 2022

The Toxicity and Antibacterial Effects of Povidone-Iodine Irrigation in Fracture Surgery.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

Department of Orthopaedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Objective: Surgical site infection is a common complication of surgery, especially in orthopedics. Povidone-Iodine (PI) is one of the oldest and most commonly used disinfectants in surgery. However, the toxicity and antimicrobial effect of PI have not been discussed. In addition, no study has explored the optimum PI concentration for sterilization and tissue healing. This study explores the germicidal efficacy of different concentrations PI, in addition, the toxicity and antibacterial effects of PI irrigation in fracture surgery are also discussed.

Methods: Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa (P. aeruginosa) were used to evaluate the germicidal efficacy of PI in vitro and in vivo. In vitro, the effects of PI on bacterial growth were analyzed. 2.5%, 1.25%, 0.5%, 0.25%, 0.05%, 0.025%, 0.005%, 0.0025% and 0% PI was added into the bacterial suspension, besides, the bacterial algebra and growth rate were tested. Meanwhile, the fluorescence intensity of viable bacteria was also tested to evaluate the effects of PI on bacterial survival. In vivo, first, femoral fracture with wound infection rat models were established. Second, thyroid gland sections, blood thyroxine, urinary iodine, wound local skin, muscle and bone tissue sections, serum creatinine and alanine aminotransferase, serum and bone local tissue interleukin-6 (IL-6), interleukin-10 (IL-10), bone morphogenetic protein (BMP-2), vascular endothelial growth factor (VEGF) and transforming growth factor (TGF-β1) were detected in rat femoral shaft fracture model with 5%, 2.5%, 0.5%, 0.05%, and 0% PI irrigation. Third, tissue bacteria culture was tested in rat femoral fracture with wound infection model with different concentrations PI irrigation.

Results: In vitro, 2.5%, 1.25%, 0.5% PI inhibited the growth of bacteria. 1.25%, 0.5% PI killed all the bacteria, while 0.25%, 0.05% PI had not killed bacteria after about 10 min. The iodine absorption of 5%, 2.5%, 0.5% PI irrigation did not cause thyroid injury. The 5%, 2.5%, 0.5% PI irrigation did not make serum creatinine and alanine aminotransferase abnormal and can remove bacteria from wounds. The 0.5%, 2.5% PI irrigation can promote tissue healing and increase BMP-2, VEGF, TGF-β1, IL-10, in addition, decrease IL-6. 5% PI irrigation would inhibit tissue healing, and increase IL-6, decrease BMP-2, VEGF, TGF-β1, IL-10.

Conclusions: Povidone-Iodine was a widely used disinfectant and 2.5%, 1.25% and 0.5% PI could effectively kill bacteria. Five percent and lower concentration PI irrigation was safe and could not cause thyroid, kidney and liver damage. The 0.5% PI irrigation was beneficial for tissue healing but 5% PI irrigation was the opposite.
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http://dx.doi.org/10.1111/os.13422DOI Listing
August 2022

A novel endoscopic suture strategy using barbed string.

Endoscopy 2022 Jul 29. Epub 2022 Jul 29.

Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1055/a-1883-9943DOI Listing
July 2022

Myeloid-Derived Suppressor Cells in Patients With Acute Pancreatitis With Increased Inhibitory Function.

Front Immunol 2022 14;13:840620. Epub 2022 Jul 14.

Department of Hepatology, The First Hospital of Jilin University, Changchun, China.

Acute pancreatitis (AP) is pancreatic or systemic inflammation without or with motion organ dysfunction. Severe acute pancreatitis (SAP) is the main cause of death for patients with AP. A pro-/anti-inflammatory imbalance is considered the key regulation of disease severity. However, the real mechanism of SAP remains unclear. This study aimed to identify the frequency and specific roll of myeloid-derived suppressor cell (MDSC) in AP. We evaluated MDSC frequency and disease severity by analyzing MDSCs in the peripheral blood of healthy controls (HCs) and patients with mild acute pancreatitis (MAP) and SAP by flow cytometry. We also compared the frequency and inhibitory ability of MDSCs from HCs and SAP, and finally detected the reason for the difference in inhibitory ability. AP was marked by expansion of MDSCs as well as its subsets, granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs). The proportion of MDSC in the peripheral blood mononuclear cells of patients with AP was increased and positively correlated with AP severity. The frequency of MDSC was decreased after treatment compared with pre-treatment. CD3+ T cells were remarkably inhibited by MDSC derived from the patients with SAP. In the expression of arginase-1 (Arg-1) and reactive oxygen species (ROS), the MDSCs from patients with SAP increased. These findings demonstrated that MDSCs expanded in the peripheral blood in patients with AP, especially in those with SAP. Moreover, the inhibitory ability of MDSCs was increased in the patients with SAP compared with that in the HCs. The enhanced suppressive function was possibly caused by an overexpression of Arg-1 and ROS.
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http://dx.doi.org/10.3389/fimmu.2022.840620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329796PMC
July 2022

Cold Plasma Irradiation Attenuates Atopic Dermatitis Enhancing HIF-1α-Induced MANF Transcription Expression.

Front Immunol 2022 14;13:941219. Epub 2022 Jul 14.

Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.

Cold atmospheric plasma has been widely applied in medical treatment clinically, especially skin diseases. However, the mechanism of cold atmospheric plasma on the treatment of skin diseases is still undefined. In this study, dinitrofluorobenzene-induced atopic dermatitis mice model was constructed. Cold atmospheric plasma was able to decrease skin cells apoptosis, relieve skin inflammation, ER stress and oxidative stress caused by dinitrofluorobenzene stimulation, which was mediated by cold atmospheric plasma-induced MANF expression. In terms of mechanism, hypoxia-inducible factor-1α expression was increased intracellularly after cold atmospheric plasma treatment, which further bound to the promoter region of gene and enhanced MANF transcriptional expression. This study reveals that cold atmospheric plasma has a positive effect on atopic dermatitis treatment, also demonstrates the regulatory mechanism of cold atmospheric plasma on MANF expression HIF-1α, which indicates the potential medical application of cold atmospheric plasma for atopic dermatitis treatment.
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http://dx.doi.org/10.3389/fimmu.2022.941219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329666PMC
August 2022

Belowground Root Competition Alters the Grass Seedling Establishment Response to Light by a Nitrogen Addition and Mowing Experiment in a Temperate Steppe.

Front Plant Sci 2022 14;13:801343. Epub 2022 Jul 14.

International Joint Research Laboratory of Global Change Ecology, School of Life Sciences, Henan University, Kaifeng, China.

Predicting species responses to climate change and land use practices requires understanding both the direct effects of environmental factors as well as the indirect effects mediated by changes in belowground and aboveground competition. Belowground root competition from surrounding vegetation and aboveground light competition are two important factors affecting seedling establishment. However, few studies have jointly examined the effect of belowground root and light competition on seedling establishment, especially under long-term nitrogen addition and mowing. Here, we examined how belowground root competition from surrounding vegetation and aboveground light competition affect seedling establishment within a long-term nitrogen addition and mowing experiment. Seedlings of two grasses ( and ) were grown with and without belowground root competition under control, nitrogen addition, and mowing treatments, and their growth characteristics were monitored. The seedlings of the two grasses achieved higher total biomass, height, mean shoot and root mass, but a lower root/shoot ratio in the absence than in the presence of belowground root competition. Nitrogen addition significantly decreased shoot biomass, root biomass, and the survival of the two grasses. Regression analyses revealed that the biomass of the two grass was strongly negatively correlated with net primary productivity under belowground root competition, but with the intercept photosynthetic active radiation in the absence of belowground root competition. This experiment demonstrates that belowground root competition can alter the grass seedling establishment response to light in a long-term nitrogen addition and mowing experiment.
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http://dx.doi.org/10.3389/fpls.2022.801343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331913PMC
July 2022

Chloroplastic photoprotective strategies differ between bundle sheath and mesophyll cells in maize ( L.) Under drought.

Front Plant Sci 2022 14;13:885781. Epub 2022 Jul 14.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Bundle sheath cells play a crucial role in photosynthesis in C4 plants, but the structure and function of photosystem II (PSII) in these cells is still controversial. Photoprotective roles of bundle sheath chloroplasts at the occurrence of environmental stresses have not been investigated so far. Non-photochemical quenching (NPQ) of chlorophyll a fluorescence is the photoprotective mechanism that responds to a changing energy balance in chloroplasts. In the present study, we found a much higher NPQ in bundle sheath chloroplasts than in mesophyll chloroplasts under a drought stress. This change was accompanied by a more rapid dephosphorylation of light-harvesting complex II (LHCII) subunits and a greater increase in PSII subunit S (PsbS) protein abundance than in mesophyll cell chloroplasts. Histochemical staining of reactive oxygen species (ROS) suggested that the high NPQ may be one of the main reasons for the lower accumulation of ROS in bundle sheath chloroplasts. This may maintain the stable functioning of bundle sheath cells under drought condition. These results indicate that the superior capacity for dissipation of excitation energy in bundle sheath chloroplasts may be an environmental adaptation unique to C4 plants.
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http://dx.doi.org/10.3389/fpls.2022.885781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330506PMC
July 2022

Granulin as an important immune molecule involved in lamprey tissue repair and regeneration by promoting cell proliferation and migration.

Cell Mol Biol Lett 2022 Jul 30;27(1):64. Epub 2022 Jul 30.

College of Life Sciences, Liaoning Normal University, Dalian, 116081, China.

Progranulin (PGRN) is an autocrine growth factor that regulates cell proliferation, migration, wound healing, and tissue repair in mammals. Lamprey is the most primitive of the extant vertebrates and is regarded as the survivor of a once flourishing group of paleozoic vertebrates, with a history of more than 500 million years. To date, the evolutionary dynamics and the underlying function of the PGRNs remain largely unclear in lamprey. Here, we screened four genes encoding PGRNs from the genomes of Lethenteron reissneri and Petromyzon marinus, including one long form (named Lr-PGRN-L) and three short forms (named Lr-PGRN-S1, Lr-PGRN-S2, and Lr-PGRN-S3), and performed phylogenetic tree, functional domain, and synteny analyses to identify the evolutionary history of the four Lr-PGRNs. In addition, the expressions of the four Lr-pgrn family genes and the immune response against various pathogenic challenges were also investigated. We found that these genes were widely distributed in various tissues of lamprey and performed a variety of functions. Moreover, our results suggest that Lr-PGRN-S1 induces cell migration and proliferation, and is involved in repair after skin and spinal cord injury under appropriate conditions. Our findings are valuable because they improve the understanding of the evolutionary relationship of vertebrate pgrn genes, as well as providing new insights into the diverse and important roles of Lr-PGRNs.
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http://dx.doi.org/10.1186/s11658-022-00360-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338584PMC
July 2022

Craniocervical Manual Lymphatic Drainage Increases the Efficiency of Atorvastatin-Based Treatment of Chronic Subdural Hematoma.

Transl Stroke Res 2022 Jul 30. Epub 2022 Jul 30.

Department of Neurosurgery, Tianjin Medical University General Hospital, Anshan Road 154, Heping District, Tianjin, China.

The objective of this study is to explore whether craniocervical manual lymphatic drainage (cMLD) can promote hematoma absorption and increase the efficiency of atorvastatin-based conservative treatment in chronic subdural hematoma (CSDH) patients. All CSDH patients treated with atorvastatin-based therapy between October 2020 and February 2022 in our department were retrospectively screened for enrollment. The patients were divided into the control and cMLD groups according to whether cMLD was performed. Head CT or MR images in both groups were obtained before the treatment and 2 weeks and 4 weeks after the treatment. MR images of the deep cervical lymphatic nodes (dCLNs) in 23 patients were obtained in the cMLD group before and approximately 2 weeks after treatment. The volumes of the dCLNs and hematoma were calculated. The primary outcomes are the differences in hematoma volume reduction after 4 weeks of treatment. The secondary outcomes were (1) the differences in hematoma volume reduction between the patients in these two groups in the 2nd week, (2) the dCLN volume change in the cMLD group before and after 2 weeks of treatment, and (3) the percentage of patients who transitioned to surgery because of failure to the conservative treatment. A total of 106 consecutive patients were enrolled in this study for analysis; 54 patients received atorvastatin-based treatment (control group), and 52 were treated with both atorvastatin-based treatment and cMLD (cMLD group). At baseline, the mean hematoma volume was 76.53 ± 42.97 ml in the control group and 88.57 ± 49.01 ml in the cMLD group (p = 0.181). In the 4th week, the absolute number of hematoma reductions (20.79 ± 34.73 ml vs. 37.28 ± 28.24 ml, p = 0.009) and percentage of hematoma reductions (22.58% ± 60.01% vs. 46.43% ± 30.12%, p = 0.012) in the cMLD group were greater than those in the control group. After 2 weeks of treatment, the absolute number of hematoma reductions showed no difference in the two groups, while the percentage of hematoma reduction was higher in the cMLD group (18.18% ± 24.61% vs. 2.08% ± 25.72%, p = 0.001). One patient in cMLD and 8 patients in the control group were transitioned to receive surgical treatment. The dCLN volumes in 23 experimental patients increased significantly after 2 weeks of treatment in the cMLD group (p = 0.032). There were no severe side effects that needed to be reported. Combined with atorvastatin-based therapy, cMLD can promote hematoma absorption and decrease the surgery rate, which provides a new therapeutic strategy for CSDH.
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http://dx.doi.org/10.1007/s12975-022-01062-zDOI Listing
July 2022

Prognostic Value of HPV E6 and APOBEC3B in Upper Urinary Tract Urothelial Carcinoma.

Dis Markers 2022 19;2022:2147494. Epub 2022 Jul 19.

Cancer Center, Daping Hospital & Army Medical Center of PLA, Third Military Medical University (Army Medical University), Chongqing, China.

Background: APOBEC mutation signature is common in upper urinary tract urothelial carcinoma (UTUC). When virus infection occurs, upregulated APOBEC plays an antiviral role by deoxycytidine deaminase activity. However, the carcinogenic roles of HPV E6 protein and APOBEC mutation signature in UTUC have not been investigated.

Aims: This study explored the relationship among HPV E6, APOBEC, and clinicopathological characteristics in patients with UTUC and impacts of their expression on the prognosis.

Methods: The expression of HPV E6 and APOBEC3B of 78 patients with UTUC was detected by immunohistochemistry. Correlation of HPV E6 and APOBEC3B expression levels with clinicopathological characteristics was statistically analyzed. Univariate and multivariate Cox regression analyses were used to evaluate the prognosis of HPV E6 and APOBEC3B for disease-free survival (DFS); survival analysis was performed using Kaplan-Meier methods.

Results: The expression of APOBEC3B was positively correlated with the expression of HPV E6 ( = 0.383, = 0.001). HPV E6 was significantly increased in patients with stage I ( = 4.938, = 0.026) and low-grade urothelial carcinoma ( = 3.939, = 0.047), as well as in patients without LVI ( = 4.064, = 0.044). Meanwhile, APOBEC3B was highly expressed in patients with stage I ( = 4.057, = 0.044) and low-grade urothelial carcinoma ( = 7.153, = 0.007). Multivariate Cox regression analysis revealed the APOBEC3B expression was the independent prognostic factor for DFS, Kaplan-Meier survival analysis showed that low expression of APOBEC3B and HPV E6 was significantly associated with the poor prognosis of UTUC patients.

Conclusion: HPV E6 expression is positively associated with APOBEC3B expression, and the high expression of HPV E6 and APOBEC3B is associated with favorable prognosis of patients with UTUC.
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http://dx.doi.org/10.1155/2022/2147494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325345PMC
August 2022

Leukocyte as an Independent Predictor of Lower-Extremity Deep Venous Thrombosis in Elderly Patients With Primary Intracerebral Hemorrhage.

Front Neurol 2022 12;13:899849. Epub 2022 Jul 12.

Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou, China.

Objective: Due to the interaction of leukocytes with platelets and coagulation factors, they may in turn play a role in hemostasis or the formation of thrombi. This study aimed to investigate the association of leukocytosis on admission with an increased risk of acute lower-extremity deep venous thrombosis (LEDVT) in elderly patients with primary intracerebral hemorrhage (ICH).

Methods: This was a single-center, retrospective observational study of consecutive patients observed with spontaneous ICH aged 60 years or above at Lanzhou University Second Hospital from January 2017 to September 2021. Clinical data and demographic information were collected and analyzed. Univariate and multivariate analyses were conducted to identify independent risk factors of acute LEDVT. One-to-one matching was implemented to balance important patient characteristics by the groups' propensity score matching (PSM) analysis.

Results: A total of 371 elderly patients with primary ICH fulfilled requirements for inclusion and exclusion, of whom 33 (8.89%) experienced LEDVT. Leukocyte counts were statistically higher in the LEDVT group compared to the non-LEDVT group [12.89 (8.80-14.61) × 10 cells/L vs. 8.31 (6.60-10.75) × 10 cells /L, < 0.001]. Multivariate logistic regression models adjusted for several potential confounding factors were performed, and leukocytes were consistently a significant independent predictor of LEDVT. The optimal cut-off value of leukocyte counts calculated from the receiver operating characteristic (ROC) curve to predict LEDVT was 10.22 × 10 cells /L (area under the curve:0.714, 95%CI 0.665-0.759; the sensitivity was 72.73%; the specificity was 71.01%) in elderly patients with primary ICH. After one-to-one PSM, compared to the matched non-LEDVT group, the matched LEDVT group had significantly higher leukocyte counts [11.98 (8.40-13.94) × 10 cells/L vs. 6.12 (4.68-12.00) × 10 cells/L, = 0.003]. After PSM, the ROC curve was plotted for leukocytes as a predictor of LEDVT, with an AUC of 0.722 (95%CI 0.593-0.828, = 0.001; the sensitivity was 87.10%, and the specificity was 61.29%). Elevated leukocytes remained independently significant as predictors of LEDVT in elderly patients with primary ICH.

Conclusion: Leukocyte at admission is an independent risk factor of LEDVT in elderly patients with primary ICH.
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http://dx.doi.org/10.3389/fneur.2022.899849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314880PMC
July 2022

Nanolab in a Cell: Crystallization-Induced Self-Assembly for Cancer Theranostic Amplification.

J Am Chem Soc 2022 Aug 28;144(31):14388-14395. Epub 2022 Jul 28.

Center for AIE Research, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China.

Conducting crystallization-assisted self-assembly in living biosystems to obtain large-size nanoparticles and achieve a specific physiological purpose remains an appealing yet significantly challenging task. In this study, we designed Au(I)-disulfide nanosheets containing an aggregation-induced emission photosensitizer, namely, [email protected], which exhibited pH-responsive crystallization-driven self-assembly capability in lysosomes of cancer cells and tumor tissues of mice. The crystallization process endowed [email protected] with a microscale morphology, stronger fluorescence output, and highly enhanced reactive oxygen species production efficiency. The results demonstrated that [email protected] shows both long-term retention in tumors and extensive destruction to cancer cells, making it supremely powerful for fluorescence imaging-guided tumor tracking and inhibition.
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http://dx.doi.org/10.1021/jacs.2c06111DOI Listing
August 2022

Plasma-Assisted Fabrication of Molecularly Imprinted NiAl-LDH Layer on Ni Nanorod Arrays for Glyphosate Detection.

ACS Appl Mater Interfaces 2022 Aug 27;14(31):35704-35715. Epub 2022 Jul 27.

Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, 100124 Beijing, People's Republic of China.

An inorganic-framework molecularly imprinted NiAl layered double hydroxide (MI-NiAl-LDH) with specific template molecule (glyphosate pesticide, Glyp) recognition ability was prepared on Ni nanorod arrays (Ni NRAs) through electrodeposition followed by a low-temperature O plasma treatment. The freestanding Ni/MI-NiAl-LDH NRA electrode had highly enhanced sensitivity and selectivity. The electrocatalytic oxidation of Glyp was proposed to occur at Ni centers in MI-NiAl-LDH, and the current response depended linearly on the Glyp concentration from 10.0 nmol/L to 1.0 μmol/L ( = 0.9906), with the limit of detection (LOD) being 3.1 nmol/L (S/N = 3). An exceptional discriminating capability with tolerance for other similar organophosphorus compounds was achieved. Molecular imprinting (N and P residues) affected the electronic structure of NiAl-LDH, triggering the formation of highly active NiOOH sites at relatively lower anodic potentials and substantially enhancing the electrocatalytic oxidation ability of the NiAl-LDH interface toward the C-N bonds in Glyp. In combination with the surface enrichment effect of MI-NiAl-LDH toward template molecules, the electrochemical oxidation signal intensity of Glyp increased significantly, with a greater peak separation from interfering molecules. These results challenge the common belief that the excellent performance of inorganic-framework molecularly imprinted interfaces arises from their specific adsorption of template molecules, providing new insight into the development of high-performance organic-pollutant-sensing electrodes.
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http://dx.doi.org/10.1021/acsami.2c08500DOI Listing
August 2022

Blue light-induced phosphorylation of Arabidopsis CRY1 is essential for its photosensitivity.

J Integr Plant Biol 2022 Jul 27. Epub 2022 Jul 27.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Plants possess two cryptochrome photoreceptors, cryptochrome 1 (CRY1) and cryptochrome 2 (CRY2), that mediate overlapping and distinct physiological responses. Both CRY1 and CRY2 undergo blue light-induced phosphorylation, but the molecular details of CRY1 phosphorylation remain unclear. Here we identify 19 in vivo phosphorylation sites in CRY1 by mass spectrometry and systematically analyze the physiological and photobiochemical activities of CRY1 variants with phosphosite substitutions. We demonstrate that nonphosphorylatable CRY1 variants have impaired phosphorylation, degradation, and physiological functions, whereas phosphomimetic variants mimic the physiological functions of phosphorylated CRY1 to constitutively inhibit hypocotyl elongation. We further demonstrate that phosphomimetic CRY1 variants exhibit enhanced interaction with the E3 ubiquitin ligase COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1). This finding is consistent with the hypothesis that phosphorylation of CRY1 is required for COP1-dependent signaling and regulation of CRY1. We also determine that PHOTOREGULATORY PROTEIN KINASEs (PPKs) phosphorylate CRY1 in a blue light-dependent manner and that this phosphorylation is critical for CRY1 signaling and regulation. These results indicate that, similar to CRY2, blue light-dependent phosphorylation of CRY1 determines its photosensitivity. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13331DOI Listing
July 2022
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