Publications by authors named "Dong Qiu"

111 Publications

Brain-targeting delivery of MMB4 DMS using carrier-free nanomedicine [email protected]

Drug Deliv 2021 Dec;28(1):1822-1835

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China.

Brain-targeting delivery of 1,1'-methylenebis[4-[(hydroxyimino)methyl]-pyridinium] dimethanesulfonate (MMB4 DMS) is limited by its hydrophilic property and chemical instability. In order to solve this problem, herein, we develop a facile protocol through combining antisolvent precipitation and emulsion-solvent evaporation method to synthesize midazolam (MDZ) coated MMB4 DMS ([email protected]) nanoparticles. The as-prepared [email protected] had a MMB4 DMS nanocrystal (MMB4-NC) core and a MDZ shell. The MDZ shell prevented the MMB4-NC core from contacting the aqueous environment, and thus, guaranteed the chemical stability of MMB4 DMS. Most charmingly, the iron mimic cyclic peptide CRTIGPSVC (CRT) was modified on [email protected] surfaces to produce [email protected] which was endowed with ability to absorb transferrin (Tf)-abundant corona. Taking advantages of the Tf-abundant corona, [email protected] achieved transferrin receptor (TfR)-mediated brain-targeting delivery. With the fascinating chemical stability and brain-targeting delivery effect, [email protected] showed great clinical transform prospect as a brand-new nanomedicine. Of particular importance, this work promised not only a core-shell carrier-free nanomedicine platform for effective delivery of unstable water-soluble drug, but also a protein corona-manipulating strategy for targeting delivery.
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http://dx.doi.org/10.1080/10717544.2021.1968977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439216PMC
December 2021

Co-immunization with L-Myc enhances CD8 or CD103 DCs mediated tumor-specific multi-functional CD8 T cell responses.

Cancer Sci 2021 Sep 10;112(9):3469-3483. Epub 2021 Jul 10.

Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, China.

Renal carcinoma shows a high risk of invasion and metastasis without effective treatment. Herein, we developed a chitosan (CS) nanoparticle-mediated DNA vaccine containing an activated factor L-Myc and a tumor-specific antigen CAIX for renal carcinoma treatment. The subcutaneous tumor models were intramuscularly immunized with CS-pL-Myc/pCAIX or control vaccine, respectively. Compared with single immunization group, the tumor growth was significantly suppressed in CS-pL-Myc/pCAIX co-immunization group. The increased proportion and mature of CD11c DCs, CD8 CD11c DCs and CD103 CD11c DCs were observed in the splenocytes from CS-pL-Myc/pCAIX co-immunized mice. Furthermore, the enhanced antigen-specific CD8 T lymphocyte proliferation, cytotoxic T lymphocyte (CTL) responses, and multi-functional CD8 T cell induction were detected in CS-pL-Myc/pCAIX co-immunization group compared with CS-pCAIX immunization group. Of note, the depletion of CD8 T cells resulted in the reduction of CD8 T cells or CD8 CD11c DCs and the loss of anti-tumor efficacy induced by CS-pL-Myc/pCAIX vaccine, suggesting the therapeutic efficacy of the vaccine was required for CD8 DCs and CD103 DCs mediated CD8 T cells responses. Likewise, CS-pL-Myc/pCAIX co-immunization also significantly inhibited the lung metastasis of renal carcinoma models accompanied with the increased induction of multi-functional CD8 T cell responses. Therefore, these results indicated that CS-pL-Myc/pCAIX vaccine could effectively induce CD8 DCs and CD103 DCs mediated tumor-specific multi-functional CD8 T cell responses and exert the anti-tumor efficacy. This vaccine strategy offers a potential and promising approach for solid or metastatic tumor treatment.
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http://dx.doi.org/10.1111/cas.15044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409417PMC
September 2021

Transcatheter aortic valve replacement in bicuspid valves: The synergistic effects of eccentric and incomplete stent deployment.

J Mech Behav Biomed Mater 2021 09 7;121:104621. Epub 2021 Jun 7.

Department of Mechanical and Materials Engineering, University of Denver, Denver, CO, USA. Electronic address:

Bicuspid aortic valve is a congenital cardiac anomaly and common etiology of aortic stenosis. Given the positive outcomes of transcatheter aortic valve replacement (TAVR) in low-risk patients, TAVR will become more prevalent in the future in the treatment of severe bicuspid valve stenosis. However, asymmetrical bicuspid valve anatomy and calcification can prevent the circular and complete expansion of transcatheter aortic valves (TAVs). In previous studies, examining the impact of elliptical TAV deployment on leaflet stress distribution, asymmetric expansion of balloon-expandable intra-annular devices was studied up to an ellipticity index (long/short TAV diameter) of 1.4. However, such a high degree of eccentricity has not been observed in clinical studies with balloon-expandable devices. High degrees of stent eccentricity have been observed in self-expanding TAVs, such as CoreValve. However, CoreValve is a supra-annular device, and it was not clear if eccentric and incomplete stent deployment at the annulus would alter leaflet stress and strain distributions. This study aimed to assess the effects of eccentric and incomplete stent deployment of CoreValves in bicuspid aortic valves and compare the results to that of SAPIEN 3. Leaflet stress distribution and leaflet kinematics of 26-mm CoreValve and 26-mm SAPIEN 3 devices in bicuspid valves were obtained in a range that was observed in previous clinical studies. The results indicated that elliptical and incomplete stent deployment of TAVs increase leaflet stress and impair leaflet kinematics. The changes were more pronounced in CoreValve than SAPIEN 3. Increased leaflet stress can reduce long-term valve durability, and impaired leaflet kinematics can potentially increase blood stasis on the TAV leaflets. The study provides complementary insights into the mechanics of TAVs in bicuspid aortic valves.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104621DOI Listing
September 2021

Cellular microRNAs influence replication of H3N2 canine influenza virus in infected cells.

Vet Microbiol 2021 Jun 20;257:109083. Epub 2021 Apr 20.

Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

MicroRNAs (miRNAs) are known to play important regulatory roles in host-virus interactions. Avian-origin H3N2 canine influenza virus (CIV) has emerged as the most prevalent subtype among dogs in Asia since 2007. To evaluate the roles of host miRNAs in H3N2 CIV infection, here, miRNA profiles obtained from primary canine bronchiolar epithelial cells (CBECs) and canine alveolar macrophages (CAMCs) were compared between infected and mock-infected cells with the H3N2 CIV JS/10. It was found that the expressions of cfa-miR-125b and cfa-miR-151, which have been reported to be associated with innate immunity and inflammatory response, were significantly decreased in CIV-infected canine primary cells. Bioinformatics prediction indicated that 5' seed regions of the two miRNAs are partially complementary to the mRNAs of nucleoprotein (NP) and non-structural protein 1 (NS1) of JS/10. As determined by virus titration, quantitative real-time PCR (qRT-PCR) and western blotting, overexpression of the two miRNAs inhibited CIV replication in cell culture, while their inhibition facilitated this replication, suggesting that the two miRNAs could act as negative regulators of CIV replication. Our findings support the notion that some cellular miRNAs can influence the outcome of virus infection, which helps to elucidate the resistance of host cells to viral infection and to clarify the pathogenesis of H3N2 CIV.
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http://dx.doi.org/10.1016/j.vetmic.2021.109083DOI Listing
June 2021

Effect of polysaccharide on human retinoblastoma Y79 cell proliferation and apoptosis.

Int J Ophthalmol 2021 18;14(4):497-503. Epub 2021 Apr 18.

Department of Ophthalmology, Fuling Central Hospital, Chongqing 408000, China.

Aim: To explore the effect of the () polysaccharide on the proliferation and apoptosis of human retinoblastoma (RB) Y79 cells and its mechanism.

Methods: The refined polysaccharide was obtained using techniques such as water extraction, ethanol precipitation, and decompression concentration. The inhibition effect of the polysaccharide on the proliferation of Y79 cells was detected by cell proliferation assay. Flow cytometry was used for the detection of cell apoptosis rate and cycle change. Real-time qunatitative polymerase chain reaction (RT qPCR)and Western blotting were used to detect the expression of cell apoptosis signal pathway-related factors (caspase-3, caspase-8, and caspase-9) and cell cycle signal pathway-related factors (CDK1 and cyclinB1) at the transcriptional and translational levels.

Results: Infrared and ultraviolet spectrum scanning showed that the extracted drug was a polysaccharide with high purity. After being treated with different concentrations of polysaccharide for different periods of time, the Y79 cells showed different degrees of proliferation inhibition. Flow cytometric observations showed that the cell apoptosis rate and the proportion of cells blocked in the G2/M phase were significantly increased after polysaccharide treatment. Further analysis revealed that the mRNA and protein expression of caspase-3, caspase-8, and caspase-9 in the polysaccharide treatment groups increased significantly compared with that in the control groups, while the expression of CDK1 and cyclinB1 decreased significantly.

Conclusion: The polysaccharide could inhibit the proliferation and induce apoptosis of Y79 cells. Its possible mechanism is the upregulation of caspase-3, caspase-8, and caspase-9 expression in the cell apoptotic signaling pathway and the downregulation of CDK1 and cyclinB1 expression in the cell cycle signaling pathway.
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http://dx.doi.org/10.18240/ijo.2021.04.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025161PMC
April 2021

Recent Advances in 2D Superconductors.

Adv Mater 2021 May 26;33(18):e2006124. Epub 2021 Mar 26.

State Key Laboratory of Electronic Thin Film and Integrated Devices, University of Electronic Science and Technology of China, Chengdu, 610054, China.

The emergence of superconductivity in 2D materials has attracted much attention and there has been rapid development in recent years because of their fruitful physical properties, such as high transition temperature (T ), continuous phase transition, and enhanced parallel critical magnetic field (B ). Tremendous efforts have been devoted to exploring different physical parameters to figure out the mechanisms behind the unexpected superconductivity phenomena, including adjusting the thickness of samples, fabricating various heterostructures, tuning the carrier density by electric field and chemical doping, and so on. Here, different types of 2D superconductivity with their unique characteristics are introduced, including the conventional Bardeen-Cooper-Schrieffer superconductivity in ultrathin films, high-T superconductivity in Fe-based and Cu-based 2D superconductors, unconventional superconductivity in newly discovered twist-angle bilayer graphene, superconductivity with enhanced B , and topological superconductivity. A perspective toward this field is then proposed based on academic knowledge from the recently reported literature. The aim is to provide researchers with a clear and comprehensive understanding about the newly developed 2D superconductivity and promote the development of this field much further.
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http://dx.doi.org/10.1002/adma.202006124DOI Listing
May 2021

Absent in melanoma 2-mediating M1 macrophages facilitate tumor rejection in renal carcinoma.

Transl Oncol 2021 Apr 22;14(4):101018. Epub 2021 Jan 22.

Cancer Institute, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China; Center of Clinical Oncology, Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China. Electronic address:

Absent in melanoma 2 (AIM2) as an immune regulator for the regulation of tumor-associated macrophages (TAMs) function is unclear in tumor development. Here, the AIM2 function was investigated in TAMs-mediated malignant behaviors of renal carcinoma. The correlation analysis result showed that the AIM2 expression in TAMs was negatively correlated with the percentages of M2-like polarization phenotype in human or murine renal cancer specimens. By the cocultured assay with bone marrow-derived macrophages (BMDMs) and Renca cells, overexpression of AIM2 in macrophages enhanced the inflammasome activation and reversed the phenotype from M2 to M1. Compared with BMDMs-Ctrl cocultured group, BMDMs-AIM2 cocultured group showed reduced tumor cell proliferation and migration. The blockade of inflammasome activation by the inhibitor Ac-YVAD-CMK abrogated AIM2-mediated M1 polarization and the inhibition of tumor cell growth. To evaluate the therapeutic efficacy of AIM2-mediated M1 macrophages in vivo, BMDMs-AIM2 were intravenously injected into subcutaneous Renca-tumor mice. The results showed that the infiltration of M1 TAMs was increased and tumor growth was suppressed in BMDMs-AIM2-treated mice when compared with BMDMs-Ctrl-treat mice. Accordingly, the blockade of inflammasome activation reduced the anti-tumor activities of BMDMs-AIM2. Moreover, the lung metastases of renal carcinoma were suppressed by the administration of BMDMs-AIM2 accompanied with the reduced tumor foci. These results demonstrated that AIM2 enhanced TAMs polarization switch from anti-inflammatory M2 phenotypy to pro-inflammatory M1 through inflammasome signaling activation, thus exerting therapeutic intervention in renal carcinoma models. Our results provide a possible molecular mechanism for the modulation of TAMs polarization in tumor microenvironment and open a new potential therapeutic approach for renal cancer.
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http://dx.doi.org/10.1016/j.tranon.2021.101018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823216PMC
April 2021

Generation and properties of one strain of H3N2 influenza virus with enhanced replication.

Vet Microbiol 2021 Feb 28;253:108970. Epub 2020 Dec 28.

Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

H3N2 canine influenza virus (CIV) has been circulating in many countries since 2008. The epidemic spread of CIV could be a concern for public health because of the close contact between humans and companion animals. In this study, we used Madin-Darby canine kidney (MDCK) cells as a coinfection model of H3N2 CIV and the pandemic (2009) H1N1 influenza virus to investigate the possibility of genetic mutation or recombination. One of the resultant progeny viruses, designated as CP15, was identified with a significantly increased replication ability. For this viral strain all segments exhibit a homology close to 100 % with its parental strain A/Canine/Jiangsu/06/2010 (JS/10), except for two site mutations K156E and R201 K which occur in the receptor-binding sites of hemagglutinin (HA) and antigen binding sites of neuraminidase (NA), respectively. Virus growth in MDCK cells showed that CP15 had a higher virus titer (more than 10 times) than JS/10. Consistent with this, CP15 exhibited extensive tissue tropism and higher viral RNA loads in the spleen, kidney and lung of mice challenged with this virus compared to JS/10. However, body weight loss and lung injure score due to CP15 infection were greatly reduced. Importantly, anti-CP15 serum antibodies could confer a high neutralization activity against JS/10. These findings indicated that the CP15 strain of high replication ability represents a promising candidate to develop an efficient CIV vaccine.
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http://dx.doi.org/10.1016/j.vetmic.2020.108970DOI Listing
February 2021

Molecular level study of cadmium adsorption on dithiocarbamate modified chitosan.

Environ Pollut 2021 Feb 15;271:116322. Epub 2020 Dec 15.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. Electronic address:

It has been shown that chemical modification of chitosan with sulfur (S) functional groups could significantly enhance its chelating capability with heavy metals included Cd(II). However, a molecular level understanding has been lacking. Here, we carried out X-ray absorption fine structure (XAFS) and Fourier transformed infrared (FTIR) spectra studies to bridge this knowledge gap. The results indicate that both Cd-O/N and Cd-S bonds exist in the complex of Cd(II) with dithiocarbamate chitosan (DTC-CTS). S functional groups (dithiocarbamate) in DTC-CTS play the major role in complexation with Cd(II) and S content affects the adsorption mechanism. At low S content, Cd(II) is mainly adsorbed on DTC-CTS as an outer-sphere complex with two monodentate amino groups and two water molecules in tetrahedral configuration. At high S content, Cd adsorption dominantly occurs by formation of an inner-sphere complex with two bidentate mononuclear S ligands in tetrahedral configuration. This investigation provides information on the effectiveness and mechanisms of Cd(II) removal that is critical for evaluating modified chitosan applications for stabilization of Cd(II) in surface water, groundwater, soils and sediments.
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http://dx.doi.org/10.1016/j.envpol.2020.116322DOI Listing
February 2021

A Solvent-Exchange Strategy to Regulate Noncovalent Interactions for Strong and Antiswelling Hydrogels.

Adv Mater 2020 Dec 9;32(52):e2004579. Epub 2020 Nov 9.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Physical hydrogels from existing polymers consisting of noncovalent interacting networks are highly desired due to their well-controlled compositions and environmental friendliness; and therefore, applied as adhesives, artificial tissues, and soft machines. Nevertheless, these gels have suffered from weak mechanical strength and low water resistance. Current methodologies used to fabricate these hydrogels mainly involve the freezing-thawing process (cryogels), which are complicated in preparation and short in adjustment of polymer conformation. Here, taking the merits of noncovalent bonds in adjustability and reversibility, a solvent-exchange strategy is developed to construct a class of exogels. Based on the exchange from a good solvent subsequently to a poor one, the intra- and interpolymer interactions are initially suppressed and then recovered, resulting in dissolving and cross-linking to polymers, respectively. Key to this approach is the good solvent, which favors of a stretched polymer conformation to homogenize the network, forming cross-linked hydrogel networks with remarkable stiffness, toughness, antiswelling properties, and thus underwater adhesive performance. The exogels highlight a facile but highly effective strategy of turning the solvent and consequently the noncovalent interactions to achieve the rational design of enhanced hydrogels and hydrogel-based soft materials.
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http://dx.doi.org/10.1002/adma.202004579DOI Listing
December 2020

Wrinkled double network hydrogel via simple stretch-recovery.

Chem Commun (Camb) 2020 Nov 14;56(88):13587-13590. Epub 2020 Oct 14.

Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Research/Education Centre for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The wrinkled structures in biological tissues play a key role in nutrition transportation and organ protection and cannot be easily achieved in synthetic hydrogels using universal and convenient strategies. Wrinkles are highly desirable for the nascent applications of hydrogels in biomaterials and artificial organs. Here, we propose a strategy for inducing different viscoelastic behaviours inside a double network hydrogel to achieve regular wrinkles that are formed by the mass redistribution. The wrinkles can be designed on multiple dimensions and can be well reserved in repeated tensile loadings. These wrinkled hydrogels exhibit unusual characteristics, such as the anisotropy of mechanics and J type tensile curves. This strategy is particularly valuable for biomaterials and artificial organs and may become a universal method for designing the surface morphology of soft materials in large-scale preparation.
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http://dx.doi.org/10.1039/d0cc05469kDOI Listing
November 2020

In vitro and in vivo evaluation of the pH-neutral bioactive glass as high performance bone grafts.

Mater Sci Eng C Mater Biol Appl 2020 Nov 2;116:111249. Epub 2020 Jul 2.

Academy of Orthopedics, Guangdong Province, Orthopaedic Hospital of Guangdong Province, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510665, People's Republic of China. Electronic address:

Osteogenic and angiogenic properties are two most valued factors for bone grafting materials. Biomedical materials with synergistic promotion effects on these two properties would be highly desirable. In this study, we showed that a recently developed pH-neutral bioactive glass (PSC) possessed such characteristics. Compared to two classical biomaterials, 45S5 bioactive glass and beta-tricalcium phosphate (β-TCP), PSC markedly improved BMSCs' proliferation, migration and mineralization as well as their osteogenic and angiogenic differentiation. In vivo, PSC showed better performance on inducing bone regeneration than both 45S5 and β-TCP, as featured by elevated bone mineral density (BMD) and new bone areas. PSC also significantly promoted new blood vessels formation compared with those in control groups. Furthermore, we revealed that PSC induced osteogenic and angiogenic differentiation of BMSCs through the PI3K/Akt/HIF-1α pathway, which had not been reported before. This synergistic effect of the PI3K/Akt/HIF-1α pathway on osteogenesis and angiogenic differentiation of BMSCs suggested that biomedical materials may promote new bone formation through multiple signal pathways, thus shedding light on the future development of materials with better performance.
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http://dx.doi.org/10.1016/j.msec.2020.111249DOI Listing
November 2020

Beneficial utilization of Al/Si/O-rich solid wastes for environment-oriented ceramic membranes.

J Hazard Mater 2021 01 11;401:123427. Epub 2020 Jul 11.

Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, PR China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, PR China. Electronic address:

Wide application of traditional multilayer ceramic membrane has been severely restricted by high costs associated with rare membrane materials and high sintering temperature. In this study, typical solid wastes (coal fly ash, river sediment and sewage sludge) were adopted as raw materials to provide an Al-Si-O matrix for single-layer ceramic membranes. Phase identification shows anorthite as major crystalline phase, while bulk density and pore characteristics of the membranes varied with different raw material compositions, with flexural strengths of 40.82-71.46 MPa, and average pore size of 0.23 μm, 0.28 μm, 0.32 μm and 0.84 μm. When the membranes were applied in an oily water treatment, the oil rejection reached >98 % when using any of the four membranes with oil/water emulsion permeate flux remaining at ∼1200 L/m·h. Furthermore, the stability of ceramic membranes in harsh environmental conditions was confirmed, with negligible weight loss ratios after being corroded in acidic/alkalic media. In addition, more than 95 % of original flux can be achieved even after six cycles, which confirmed the excellent recyclability of the membranes. The successful fabrication and application of the environment-oriented single layer ceramic membranes from the Al-Si-O solid waste matrix provided a promising "waste-to-resource" strategy for beneficial utilization of typical solid wastes as ceramic raw materials.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123427DOI Listing
January 2021

Absent in melanoma 2 enhances anti-tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer.

J Cell Mol Med 2020 09 29;24(18):10744-10755. Epub 2020 Jul 29.

Center of Clinical Oncology, Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, China.

Conditionally replicative adenoviruses (CRAds) were promising approach for solid tumour treatment, but its oncolytic efficiency and toxicity are still not satisfactory for further clinical application. Here, we developed the CAIX promotor (CAIX )-controlled CRAd armed with a tumour suppressor absent in melanoma 2 (AIM2) to enhance its oncolytic potency. The CAIX -AIM2 adenoviruses (Ad-CAIX -AIM2) could efficiently express E1A and AIM2 in renal cancer cells. Compared with Ad-CAIX , Ad-CAIX -AIM2 significantly inhibited cell proliferation and enhanced cell apoptosis and cell killing, thus resulting in the oncolytic efficiency in 786-O cells or OSRC-2 cells. To explore the therapeutic effect, various Ads were intratumourally injected into OSRC-2-xenograft mice. The tumour growth was remarkably inhibited in Ad-CAIX -AIM2-treated group as demonstrated by reduced tumour volume and weight with a low toxicity. The inflammasome inhibitor YVAD-CMK resulted in the reduction of anti-tumour activity by Ad-CAIX -AIM2 in vitro or in vivo, suggesting that inflammasome activation response was required for the enhanced therapeutic efficiency. Furthermore, lung metastasis of renal cancer mice was also suppressed by Ad-CAIX -AIM2 treatment accompanied by the decreased tumour fossil in lung tissues. These results indicated that the tumour-specific Ad-CAIX -AIM2 could be applied for human renal cancer therapy. The therapeutic strategy of AIM2-based CRAds could be a potential and promising approach for the therapy of primary solid or metastasis tumours.
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http://dx.doi.org/10.1111/jcmm.15697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521288PMC
September 2020

Biphasic Double-Network Hydrogel With Compartmentalized Loading of Bioactive Glass for Osteochondral Defect Repair.

Front Bioeng Biotechnol 2020 2;8:752. Epub 2020 Jul 2.

Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, China.

Periarticular injury usually causes the defects of superficial cartilage and the underlying subchondral bone. Although some efficacious outcomes have been achieved by the existing therapeutic methods both in clinics and research, like symptomatic treatment, microfracture surgery, and tissue engineering technology, they still present specific disadvantages and complications. To improve this situation, we designed a biphasic (bi-) scaffold aiming to repair the structure of cartilage and subchondral bone synchronously. The scaffold consisted of a superior double-network (DN) hydrogel layer and a lower bioactive glass (BG) reinforced hydrogel layer, and the DN hydrogel included glycol chitosan (GC) and dibenzaldhyde functionalized poly(ethylene oxide) network, and sodium alginate (Alg) and calcium chloride (CaCl) network. To investigate its effectiveness, we applied this biphasic scaffold to repair osteochondral full-thickness defects in rabbit models. We set up six observation groups in total, including Untreated group, Microfracture group, BG only group, DN gel group, bi-DN gel group, and bi-DN/TGF-β gel group. With a follow-up period of 24 weeks, we evaluated the treatment effects by gross observation, micro-CT scan and histological staining. Besides, we further fulfilled the quantitative analysis of the data from ICRS score, O'Driscoll score and micro-CT parameters. The results revealed that neat GC/Alg DN hydrogel scaffold was only conductive to promoting cartilage regeneration and neat BG scaffold merely showed the excellent ability to reconstruct subchondral bone. While the biphasic scaffold performed better in repairing osteochondral defect synchronously, exhibiting more well-integrated cartilage-like tissue with positive staining of toluidine blue and col II immunohistochemistry, and more dense trabecular bone connecting closely with the surrounding host bone. Therefore, this method possessed the clinical application potential in treating articular injury, osteochondral degeneration, osteochondral necrosis, and sclerosis.
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http://dx.doi.org/10.3389/fbioe.2020.00752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346869PMC
July 2020

Author Correction: Additive manufacturing of ultrafine-grained high-strength titanium alloys.

Nature 2020 Jun;582(7811):E5

Centre for Additive Manufacturing, School of Engineering, RMIT University, Melbourne, Victoria, Australia.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41586-020-2291-zDOI Listing
June 2020

Detection of miR-155-5p and imaging lung cancer for early diagnosis: in vitro and in vivo study.

J Cancer Res Clin Oncol 2020 Aug 23;146(8):1941-1951. Epub 2020 May 23.

Cancer Institute of PLA, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.

Purpose: Currently, the routine screening program has insufficient capacity for the early diagnosis of lung cancer. Therefore, a type of chitosan-molecular beacon (CS-MB) probe was developed to recognize the miR-155-5p and image the lung cancer cells for the early diagnosis.

Methods: Based on the molecular beacon (MB) technology and nanotechnology, the CS-MB probe was synthesized self-assembly. There are four types of cells-three kinds of animal models and one type of histopathological sections of human lung cancer were utilized as models, including A549, SPC-A1, H446 lung cancer cells, tumor-initiating cells (TICs), subcutaneous and lung xenografts mice, and lox-stop-lox(LSL) K-ras G12D transgenic mice. The transgenic mice dynamically displayed the process from normal lung tissues to atypical hyperplasia, adenoma, carcinoma in situ, and adenocarcinoma. The different miR-155-5p expression levels in these cells and models were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The CS-MB probe was used to recognize the miR-155-5p and image the lung cancer cells by confocal microscopy in vitro and by living imaging system in vivo.

Results: The CS-MB probe could be used to recognize the miR-155-5p and image the lung cancer cells significantly in these cells and models. The fluorescence intensity trends detected by the CS-MB probe were similar to the expression levels trends of miR-155 tested by qRT-PCR. Moreover, the fluorescence intensity showed an increasing trend with the tumor progression in the transgenic mice model, and the occurrence and development of lung cancer were dynamically monitored by the differen fluorescence intensity. In addition, the miR-155-5p in human lung cancer tissues could be detected by the miR-155-5p MB.

Conclusion: Both in vivo and in vitro experiments demonstrated that the CS-MB probe could be utilized to recognize the miR-155-5p and image the lung cancer cells. It provided a novel experimental and theoretical basis for the early diagnosis of the disease. Also, the histopathological sections of human lung cancer research laid the foundation for subsequent preclinical studies. In addition, different MBs could be designed to detect other miRNAs for the early diagnosis of other tumors.
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http://dx.doi.org/10.1007/s00432-020-03246-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324423PMC
August 2020

Large scale synthesis of single-chain/colloid Janus nanoparticles with tunable composition.

Chem Commun (Camb) 2020 Apr 5;56(27):3875-3878. Epub 2020 Mar 5.

State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

A general approach is proposed to large scale synthesize tadpole-like composite Janus nanoparticles (JNPs). Within the head of a starting JNP achieved by electrostatic-mediated intramolecular crosslinking, a free radical initiator is incorporated. By polymerizing vinyl monomers within the head nanoreactor, the composition and performance of the JNPs are broadly tunable.
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http://dx.doi.org/10.1039/d0cc00686fDOI Listing
April 2020

Bioactive Pore-Forming Bone Adhesives Facilitating Cell Ingrowth for Fracture Healing.

Adv Mater 2020 Mar 27;32(10):e1907491. Epub 2020 Jan 27.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

The effectiveness of commercial bone adhesives is known to be hampered by the weak efficacy of cell ingrowth. The strategy of macropore-forming, especially bioactive macropores, holds considerable promise to circumvent this problem, thereby promoting fracture healing. Herein, a class of bioactive glass-involved macropore-embedded bone adhesives is developed, which is capable of facilitating the migration of bone-derived mesenchymal stromal cells into the adhesive layer and differentiation into osteocytes. The integration of bioactive glass-particle-encapsulated porogens in the bone adhesives is key to this approach. A robust instant bonding on the bone adhesive and a high efficiency of bone regeneration on a mouse skull are observed, both of which are vital for clinical applications and personalized surgical procedures. This work represents a general strategy to design biomaterials with high cell-ingrowth efficacy.
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http://dx.doi.org/10.1002/adma.201907491DOI Listing
March 2020

Additive manufacturing of ultrafine-grained high-strength titanium alloys.

Nature 2019 12 4;576(7785):91-95. Epub 2019 Dec 4.

Centre for Additive Manufacturing, School of Engineering, RMIT University, Melbourne, Victoria, Australia.

Additive manufacturing, often known as three-dimensional (3D) printing, is a process in which a part is built layer-by-layer and is a promising approach for creating components close to their final (net) shape. This process is challenging the dominance of conventional manufacturing processes for products with high complexity and low material waste. Titanium alloys made by additive manufacturing have been used in applications in various industries. However, the intrinsic high cooling rates and high thermal gradient of the fusion-based metal additive manufacturing process often leads to a very fine microstructure and a tendency towards almost exclusively columnar grains, particularly in titanium-based alloys. (Columnar grains in additively manufactured titanium components can result in anisotropic mechanical properties and are therefore undesirable.) Attempts to optimize the processing parameters of additive manufacturing have shown that it is difficult to alter the conditions to promote equiaxed growth of titanium grains. In contrast with other common engineering alloys such as aluminium, there is no commercial grain refiner for titanium that is able to effectively refine the microstructure. To address this challenge, here we report on the development of titanium-copper alloys that have a high constitutional supercooling capacity as a result of partitioning of the alloying element during solidification, which can override the negative effect of a high thermal gradient in the laser-melted region during additive manufacturing. Without any special process control or additional treatment, our as-printed titanium-copper alloy specimens have a fully equiaxed fine-grained microstructure. They also display promising mechanical properties, such as high yield strength and uniform elongation, compared to conventional alloys under similar processing conditions, owing to the formation of an ultrafine eutectoid microstructure that appears as a result of exploiting the high cooling rates and multiple thermal cycles of the manufacturing process. We anticipate that this approach will be applicable to other eutectoid-forming alloy systems, and that it will have applications in the aerospace and biomedical industries.
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http://dx.doi.org/10.1038/s41586-019-1783-1DOI Listing
December 2019

Coupling of Fluorophores in Single Nanoapertures to Tamm Plasmon Structures.

J Phys Chem C Nanomater Interfaces 2019 Jan 29;123(2):1413-1420. Epub 2018 Dec 29.

Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, MD 21201, United States.

Metal nanostructures (such as plasmonic antennas) have been widely demonstrated to be excellent devices for beaming and sorting the fluorescence emission. These effects rely on the constructive scattering or diffraction from different elements (such as metal corrugations or nanorings) of the nanostructures. However, subwavelength-size nanoholes, without nearby nanoscale features, results in an angularly dispersed emission from the distal surface. Herein, we demonstrate for the first time the emission redirection capabilities of a single isolated nanoaperture milled in a thick silver film deposited on a dielectric multilayer. Specifically, we show that a dye dissolved in ethanol filling in the nanoaperture can couple to Tamm Plasmon Polariton (TPP) modes of the structure. Due to the small in-plane wavevectors of the TPPs, the fluorescence from Tamm-coupled dyes within the nanoaperture is emitted normally to the sample surface, with a minimum angular width of about 12.54°. This kind of fluorescence manipulation has proven to be effective with various nanoaperture shapes, such as circles, squares, and triangles. Our work is also the first experimental demonstration of lateral coupling of fluorophores with TPPs in nanoholes, with potential applications in bioanalysis and biosciences.
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http://dx.doi.org/10.1021/acs.jpcc.8b11498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824544PMC
January 2019

Response-Retaliation Behavior in Synthetic Protocell Communities.

Angew Chem Int Ed Engl 2019 12 22;58(49):17758-17763. Epub 2019 Oct 22.

Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.

Two different artificial predation strategies are spatially and temporally coupled to generate a simple tit-for-tat mechanism in a ternary protocell network capable of antagonistic enzyme-mediated interactions. The consortium initially consists of protease-sensitive glucose-oxidase-containing proteinosomes (1), non-interacting pH-sensitive polypeptide/mononucleotide coacervate droplets containing proteinase K (2), and proteinosome-adhered pH-resistant polymer/polysaccharide coacervate droplets (3). On receiving a glucose signal, secretion of protons from 1 triggers the disassembly of 2 and the released protease is transferred to 3 to initiate a delayed contact-dependent killing of the proteinosomes and cessation of glucose oxidase activity. Our results provide a step towards complex mesoscale dynamics based on programmable response-retaliation behavior in artificial protocell consortia.
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http://dx.doi.org/10.1002/anie.201909313DOI Listing
December 2019

The Influence of Occupational Noise Exposure on Cardiovascular and Hearing Conditions among Industrial Workers.

Sci Rep 2019 08 8;9(1):11524. Epub 2019 Aug 8.

Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210028, China.

This study was conducted to estimate the current prevalence of hypertension, cardiovascular condition and hearing difficulty of workers exposure to occupational noise, and to analyze any associations between these abnormal signs and occupational noise exposure. The subjects included 5205 noise-exposed workers. Workers with high noise exposure were more likely to have a higher threshold value than low exposure ones (P < 0.05). Subjects in the high exposure group had a significantly higher risk of hypertension and hearing loss than the ones in low exposure group. Between the ages of 30 and 45, high-level occupational noise exposure led to a significantly raising risk of both hypertension (Adjusted OR = 1.59, 95% CI, 1.19-2.11) and hearing loss (Adjusted OR = 1.28, 95% CI, 1.03-1.60) when comparing to low-level noise exposure. In male workers, the prevalence of hearing difficulty in high exposure group was approximately 1.2 times worse than in low group (P = 0.006). In addition, exposure to high noise level demonstrated a significant association with hypertension and hearing loss when the duration time to occupational noise was longer than 10 years. Hypertension and hearing difficulty is more prevalent in the noise-exposed group (higher than 85 dB[A]). Steps to reduce workplace noise levels and to improve workplace-based health are thus urgently needed.
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http://dx.doi.org/10.1038/s41598-019-47901-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687890PMC
August 2019

An Injectable Strong Hydrogel for Bone Reconstruction.

Adv Healthc Mater 2019 09 28;8(17):e1900709. Epub 2019 Jul 28.

Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

For treating bone defects in periarticular fractures, there is a lack of biomaterial with injectable characteristics, tough structure, and osteogenic capacity for providing a whole-structure support and osteogenesis in the defect area. An injectable hydrogel is an ideal implant, however is weak as load-bearing scaffolds. Herein, a new strategy, i.e., an in situ formation of "active" composite double network (DN), is raised for the preparation of an injectable strong hydrogel particularly against compression. As a demonstration, 4-carboxyphenylboronic acid grafted poly(vinyl alcohol) (PVA) is crosslinked using calcium ions to provide a tough frame while bioactive glass (BG) microspheres are associated by poly(ethylene glycol) to obtain an interpenetrated inorganic network for reinforcement. The injected PVA/BG DN hydrogel gains compressive strength, modulus, and fracture energy of 34 MPa, 0.8 MPa, and 40 kJ m , respectively. Then, the properties can be "autostrengthened" to 57 MPa, 2 MPa, and 65 kJ m by mineralization in 14 days. In vivo experiments prove that the injected DN hydrogel is more efficient to treat femoral supracondylar bone defects than the implanted bulk DN gel. The work suggests a facile way to obtain a strong hydrogel with injectability, cytocompatibility, and tailorable functionality.
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http://dx.doi.org/10.1002/adhm.201900709DOI Listing
September 2019

Bioactive Glass-Polycitrate Hybrid with Osteogenetic Ability Comparable to Autogenous Bone.

J Biomed Nanotechnol 2019 Mar;15(3):581-592

Significant progress in artificial bone grafts has been achieved in recent years. However, none of them has osteogenic ability that is close to autogenous bones. Hence, improving osteogenic ability of artificial bone is the most prominent and challenging task in this field. In addition, angiogenesis could provide a stable environment and nutrients for survival of the cells and also plays a crucial role to the success of bone transplantation. In the present study, we combined citrate polymer and bioactive glass together as hybrids at the molecular level (PEC-GS/BG), with the expectation of acquiring osteogenic ability and angiogenic ability to repair bone defect that could comparable to autogenous bones. and experiment on the femoral condyle critical defects model of Sprague-Dawley rats were conducted for a complete evaluation. , the bone mineral density (BMD) in defects was no significant difference between autogenous bone groups (517 ± 21 mg/cm³) and PEC-GS/BG groups (509 ± 21 mg/cm³) ( > 0 05) at 12 weeks post-surgery. The BMD of the femoral condyle in normal males at the same age was measured to be 557 ± 16 mg/cm³, only slightly higher than the above date, indicating a nearly complete repair of the defects. It was also found that PEC-GS/BG promoted angiogenesis due to it stimulated organism to release vascular endothecial growth factor (VEGF). PEC-GS/BG also showed great osteogenic ability that was close to autogeneous bones, but much better angiogenic ability. What's more, from both protein and cell levels, PEC-GS/BG accelerated differentiation and mineralization of MC3T3E-1 cells. Consequently, osteogenetic performance of PEC-GS/BG was almost same to autogenous bones in repairing bone defects. Considering the high demand in bone grafts and all the difficulties in autogeneous bone supply, the PEC-GS/BG hybrids developed in this study may open a new horizon for bone repair.
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http://dx.doi.org/10.1166/jbn.2019.2709DOI Listing
March 2019

Reducing the risk of leaflet thrombosis in transcatheter aortic valve-in-valve implantation by BASILICA: a computational simulation study.

EuroIntervention 2019 May;15(1):67-70

The DU Cardiovascular Biomechanics Laboratory, Department of Mechanical and Materials Engineering, University of Denver, Denver, CO, USA.

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http://dx.doi.org/10.4244/EIJ-D-19-00048DOI Listing
May 2019

Chemotherapy combined with Endostar as salvage treatment for EGFR-tyrosine kinase inhibitor primary resistance in an advanced non-small cell lung cancer patient with EGFR L858R mutation and ROS1 fusion: A case report.

Thorac Cancer 2019 04 18;10(4):1023-1028. Epub 2019 Feb 18.

Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China.

EGFR-activating mutations have been recognized as the most important predictor of response to EGFR-tyrosine kinase inhibitors (TKIs); however, 20-30% of patients harboring EGFR-activating mutations show poor responses. The mechanisms of such EGFR-TKI primary resistance are still poorly understood. In our case, a non-small cell lung cancer patient developed intrinsic EGFR-TKI resistance and was then confirmed to simultaneously harbor an L858R mutation and ROS1 rearrangement. Salvage chemotherapy plus Endostar showed enduring therapeutic effects, achieving a disease-free survival period of 24 months and overall survival of 30 months. This suggests that co-activation of different oncogenic signal pathways might be a potential mechanism of EGFR-TKI primary resistance. Chemotherapy combined with anti-angiogenesis should be considered an important salvage strategy. Further studies are warranted to verify these findings and explore the underlying mechanisms involved.
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http://dx.doi.org/10.1111/1759-7714.13003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449238PMC
April 2019

Conjoined-network rendered stiff and tough hydrogels from biogenic molecules.

Sci Adv 2019 02 1;5(2):eaau3442. Epub 2019 Feb 1.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Hydrogels from biological sources are expected as potential structural biomaterials, but most of them are either soft or fragile. Here, a new strategy was developed to construct hydrogels that were both stiff and tough via the formation of the conjoined-network, which was distinct from improving homogeneity or incorporating energy dissipation mechanisms (double-network) approaches. Conjoined-network hydrogels stand for a class of hydrogels consisting of two or more networks that are connected by sharing interconnection points to collaborate and featured as follows: (i) All the composed networks had a similar or equal energy dissipation mechanism, and (ii) these networks were intertwined to effectively distribute stress in the whole system. As a specific example, a biogenic conjoined-network hydrogel was prepared by electrostatically cross-linking the chitosan-gelatin composite with multivalent sodium phytate. The combination of high compressive modulus and toughness was realized at the same time in the chitosan-gelatin-phytate system. Moreover, these physical hydrogels exhibited extraordinary self-recovery and fatigue resistance ability. Our results provide a general strategy for the design of biocompatible stiff and tough conjoined-network hydrogels due to a variety of potential cross-linking mechanisms available (e.g., electrostatic attraction, host-guest interaction, and hydrogen bonding).
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http://dx.doi.org/10.1126/sciadv.aau3442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358320PMC
February 2019

Electrochromism of Nanocrystal-in-Glass Tungsten Oxide Thin Films under Various Conduction Cations.

Inorg Chem 2019 Feb 15;58(3):2089-2098. Epub 2019 Jan 15.

Key Laboratory of Graphene Technologies and Applications of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering , Chinese Academy of Sciences , Ningbo 315201 , People's Republic of China.

The nanocrystal-in-glass (nanocrystals embedded amorphous matrix) tungsten oxide (WO) thin films with a nanoporous characteristic were prepared via an electron beam evaporation technique. The e-beam evaporated WO thin films present a fast colored/bleached time of 16/11, 16/14, and 12/12 s, a large optical modulation of 92, 91, and 87% at 633 nm, and a high coloration efficiency of 61.78, 62.04, and 67.59 cm C in Li, Na, and Al electrolytes, respectively. On one hand, the improved electrochromic performance is mainly attributed to the short diffusion distance and buffering effect in the host matrix, which facilitates the ion insertion/extraction and alleviates the structural collapse of the framework. On the other, owing to the strong electrostatic interactions between the trivalent cations and the host, the WO thin films in Al possess a shallow diffusion depth and long cycle life. The individual contribution from the capacitance- or diffusion-controlled process is comprehensively demonstrated. Pseudocapacitive behavior in the nanocrystal-in-glass WO thin films is in favor of fast kinetics response and sound cycling stability. Our work offers an in-depth insight of the electrochromic mechanism for nanocrystal-in-glass WO thin films in various electrolytes and sheds light on the fundamental principle in the electrochromic devices.
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http://dx.doi.org/10.1021/acs.inorgchem.8b03178DOI Listing
February 2019

Regulating polymer adsorption on colloid by surface morphology.

Soft Matter 2018 Nov;14(46):9336-9342

Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The study of polymer adsorption on colloidal particles has attracted intensive attention. In this work, we investigated polymer adsorption on substrate colloidal particles with two complementary morphologies, one of which has bulges (raspberry-like) on the surface and the other of which has holes instead (strawberry-like). Compared to the bulges, the holes on the colloidal particles were found to prevent polymer adsorption and this effect was dependent on the relative dimensions of the polymer coil and hole. This surface morphology effect was attributed mainly to the reduced polymer accessibility to the adsorption sites in holes when the hydrodynamic size of the polymer coil is larger than the hole, due to the size limiting effect. When the hydrodynamic size of the polymer coil is smaller than that of the holes, no difference in polymer adsorption was observed between raspberry-like and strawberry-like colloids. This study provides a strategy for regulating polymer adsorption on colloidal particles by adjusting the fine structures on the surface, which may be advantageous when limited chemical compositions are allowed. For example, protein adsorption on colloidal drugs may be found to be significantly reduced when colloids with surface holes are used.
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http://dx.doi.org/10.1039/c8sm01833bDOI Listing
November 2018
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