Publications by authors named "Dong Liu"

1,719 Publications

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Different storm responses of organic carbon transported to Lake Taihu by the eutrophic Tiaoxi River, China.

Sci Total Environ 2021 Apr 1;782:146874. Epub 2021 Apr 1.

Key Laboratory of Watershed Geographic Sciences, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; College of Urban and Environmental Sciences, Northwest University, Xi'an 710127, China. Electronic address:

Low-frequency high-magnitude storms can flush disproportionate amounts of terrigenous dissolved organic carbon (DOC) and particulate organic carbon (POC) into rivers during a short period. However, previous studies focused on the impacts of storms on organic carbon transport in headwater streams that are minimally influenced by human activities and are far from lakes. To better estimate the lake carbon budget and manage lake water environments, we need to understand the transport of storm-induced organic carbon into lakes by eutrophic rivers. Based on daily and hourly time-series monitoring data, this paper systematically studied the influences of storm precipitation on DOC and POC transport in the eutrophic Tiaoxi River entering Lake Taihu, the 3rd largest freshwater lake in China. The results showed that seven storms transported 59% of the annual total organic carbon into Lake Taihu in 2019, and all storms resulted in transport peaks. During the storm period on August 9-16, 2019, DOC was negatively related to the water level (r = -0.44, p < 0.05), but POC responded positively (r = 0.52, p < 0.05); allochthonous organic carbon contents were elevated, but the autochthonous components were diluted. Moreover, the storm-induced input of riverine organic carbon influenced the lake water environment across a large region, and the impacts lasted more than 10 days. These findings have important implications for accurately estimating riverine organic carbon fluxes into lakes and making better-informed decisions about when to pump drinking water from lakes.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146874DOI Listing
April 2021

Interaction between the functionalized probes: The depressed efficiency of dual-amplification strategy on ratiometric electrochemical aptasensor for aflatoxin B1.

Biosens Bioelectron 2021 Mar 25;182:113169. Epub 2021 Mar 25.

Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address:

Signal amplification is one of the most effective ways to develop the high-performance electrochemical sensors. However, it can be more complicated for ratiometric detections. Herein, a ratiometric electrochemical aptasensor for aflatoxin B1 (AFB1) was proposed by taking advantage of a dual-amplification strategy by coupling of DNA walker (DW) with hybridization chain reaction (HCR). The special binding of AFB1 with ferrocene (Fc)-labelled aptamer triggers DW on hairpin DNA (hDNA) tracks to produce abundant double-stranded DNA (dsDNA). HCR-based strand amplification occurs on these dsDNA to absorb more methylene blue (MB). Then current ratio of MB (I) and Fc (I) is designed as a yardstick to detect AFB1. Our experiments reveal that the interaction between Fc and MB (i.e., steric hindrance, electron mediator) varies. In addition to steric hindrance, the presence of MB also acts as electron mediator, thereby facilitating the electron transfer between Fc and electrode. Such combined effect consequently depresses the efficiency of dual-amplification strategy to improve the detection. The developed ratiometric electrochemical aptasensor allows the accurate detection of AFB1 in the 0.003-3 pg mL range. Our work has shed light on the amplification strategy for ratiometric sensing, and provided a new route in integrating different amplification strategies.
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http://dx.doi.org/10.1016/j.bios.2021.113169DOI Listing
March 2021

The complete chloroplast genome of wild Rehd. et Wils.

Mitochondrial DNA B Resour 2021 Mar 15;6(3):880-882. Epub 2021 Mar 15.

Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, P.R. China.

Rehd. et Wils. is an important traditional Chinese herbal medicine and widely distributed in the south of Yangtze River in China. In this study, the complete chloroplast genome sequence of wild was assembled and characterized from high-throughput sequencing data. The chloroplast genome was 160,009 bp in length, consisting of large single-copy (LSC) and small single-copy (SSC) regions of 88,134 bp and 18,739 bp, respectively, which were separated by a pair of 26,568 bp inverted repeat (IR) regions. The genome is predicted to contain 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content of the genome is 39.3%. A phylogenetic tree reconstructed by 86 chloroplast genomes reveals that is mostly related to cultivated and
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http://dx.doi.org/10.1080/23802359.2021.1886011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971295PMC
March 2021

Separation performance and mechanism of the novel modified polyether sulfone composite nanofiltration membrane for the detection on dissolved organic nitrogen.

Water Environ Res 2021 Mar 29. Epub 2021 Mar 29.

Suzhou Erye Pharmaceutical Co., Ltd, Suzhou, China.

The most effective pretreatment method for the detection of dissolved organic nitrogen (DON) is the nanofiltration (NF) by effectively intercepting DON into the concentrated solution, thus increasing the proportion of DON in solution so that achieving accurate detection of DON. The existing NF pretreatment technology for detection of DON has the problem of low accuracy and unclear separation mechanism of mass transfer upon the NF membranes. Based on the problems of NF membrane, the polyether sulfone (PES) composite NF membrane was modified by mesoporous carbon and the basic performance parameters of the modified PES composite NF membrane were characterized by Static contact angle (SCA), Zeta potential, and X-ray photoelectron spectroscopy (XPS). The results show that the optimum doping amount of mesoporous carbon is in the range of 0.5%-1.0%. Furthermore, the pore size of NF membrane could be controlled effectively by changing doping amount of mesoporous carbon, so as to guide the improvement of membrane pore structure. After the pretreatment of mesoporous material modified PES composite NF membrane, the ratio of dissolved inorganic nitrogen (DIN)/total dissolved nitrogen (TDN) could be reduced to 0.55-0.65 and the pretreatment effect was also affected by screening effect and electrostatic effect. The best retention performance of amino acids is the 1.0%C modified membrane which retention rate of the amino acid is between 32.3% and 94.7%, and the membrane loss of the four amino acids is much smaller than other membranes. The mass transfer process of NF membrane was simulated by steric-hindrance pore (SHP) model, Teorell-Meyer-Sievers (TMS) model, and Electrostatic Steric-hindrance (ES) model respectively. According to the simulation software designed by ourselves, the dominant effects of screening and electrostatic effects are simultaneously affected by the pore structure of membranes and the size of the contaminant molecules. PRACTITIONER POINTS: The feasibility of mesoporous carbon with modified PES composite nanofiltration membrane pretreatment for DON measurement in comparison to pure NF membranes was investigated. The ability of modified PES composite NF membrane with different molecular weight of DON and the relationship between pore radius of membrane and interception pollutant molecular size was discussed. The influence of screening effect and electrostatic effect on the retention of pollutants was discussed by the SHP model, TMS model and ES model; the simulation software interface is designed and the calculation process of the model is simulated by computer.
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http://dx.doi.org/10.1002/wer.1562DOI Listing
March 2021

Estrogen cholestasis induces gut and liver injury in rats involving in activating PI3K/Akt and MAPK signaling pathways.

Life Sci 2021 Mar 26;276:119367. Epub 2021 Mar 26.

Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Backgrounds: Estrogen and its metabolites often lead to intrahepatic cholestasis in susceptible women with pregnancy, administration of oral contraceptives and postmenopausal hormone replacement therapy. Recently, dysfunction of the gut-liver axis has been suggested to play a pivotal role in the progression of cholestasis, but details about estrogen cholestasis (EC)-induced gut and liver injury are still largely unknown. This study aims to gain insight into EC-induced gut and liver injury and cell signaling implicated.

Methods: Male rats were exposed to 5 and 10 mg/kg of 17α-ethinylestradiol via subcutaneous injection for 5 successive days to simulate human EC.

Results: By detection of these estrogen cholestatic rats, we found that EC induced inflammation in the liver but not in the intestine through activating NF-κB signaling pathway. EC strongly induced oxidative stress in both the liver and intestine, and activated the hepatic Nrf2/Gclm/Gclc pathway and the intestinal Nrf2/Ho-1 pathway, respectively, for adaptively regulating oxidative stress. EC increased cell apoptosis in both the liver and intestine. Additionally, EC elevated phosphorylation of Akt, ERK1/2, and p38 in the liver and increased phosphorylation of p38 in the intestine.

Conclusions: EC induces liver inflammation, both gut and liver oxidative stress and apoptosis, involving in activating PI3K/Akt and MAPK signaling pathways. Investigation of EC-induced gut and liver injury contributes to the development of new potential therapeutic strategies.
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http://dx.doi.org/10.1016/j.lfs.2021.119367DOI Listing
March 2021

Radar-lidar ratio for ice crystals of cirrus clouds.

Opt Express 2021 Feb;29(3):4464-4474

Simultaneous measurement of lidar and radar signals returned from the same cirrus clouds is a prospective method for retrieving the cloud microphysics, i.e. size and shape of the ice crystals constituting the clouds. In this study, the ratio of the backscattered signals of lidar and radar called the radar-lidar ratio has been calculated for the first time for typical shapes of ice crystals and wide distribution of the crystals over their sizes. It is shown that it is the lidar-radar ratio that is most sensitive to crystal sizes while the lidar depolarization ratio is most sensitive to crystal shapes.
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http://dx.doi.org/10.1364/OE.410942DOI Listing
February 2021

Esterification of Carboxylic Acids with Aryl Halides via the Merger of Paired Electrolysis and Nickel Catalysis.

J Org Chem 2021 Mar 26. Epub 2021 Mar 26.

State Key Laboratory of Organometallic Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China.

Electrochemistry has been successfully applied in metal catalysis to avoid the usage of chemical redox agents. This strategy proved to be a powerful approach to construct carbon-carbon (C-C) and carbon-heteroatom (C-X) bonds. However, most of the developed methods are based on either anodic oxidation or cathodic reduction, in which a sacrificial reaction occurs at the counter electrode. Paired electrolysis merging with metal catalysis is underdeveloped, wherein both anodic and cathodic processes are taking place simultaneously. Herein, we demonstrated that by using esterification of carboxylic acids with aryl halides via paired electrolysis using nickel as the catalyst the respective aryl esters were obtained in good to excellent yields at room temperature in an undivided electrochemical cell.
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http://dx.doi.org/10.1021/acs.joc.1c00204DOI Listing
March 2021

Different School Reopening Plans on Coronavirus Disease 2019 Case Growth Rates in the School Setting in the United States.

J Sch Health 2021 May 25;91(5):370-375. Epub 2021 Mar 25.

Senior Epidemiologist/ Research liaison, Epidemiology Informatics, Nebraska Department of Health and Human Service, Lincoln., USA.

Background: In fall 2020, all public K-12 schools reopened in broadly 3 learning models. The hybrid model was considered a mid-risk option compared with remote and in-person learning models. The current study assesses school-based coronavirus disease 2019 (COVID-19) spread in the early fall using a national data set.

Methods: We assess COVID-19 case growth rates from August 10 to October 14, 2020 based on a crowdsourcing data set from the National Education Association. The study follows a retrospective cohort design with the baseline exposures being 3 teaching models: remote learning only, hybrid, and in-person learning. To assess the consistency of our findings, we estimated the overall, as well as region-specific (Northeast, Midwest, South, and West) and poverty-specific (low, mid, and high) COVID-19 case-growth rates. In addition, we validated our study sample using another national sample survey data.

Results: The baseline was from 617 school districts in 48 states, where 47% of school districts were in hybrid, 13% were in remote, and 40% were in-person. Controlling for state-level risk and rural-urban difference, the case growth rates for remote and in-person were lower than the hybrid (odds ratio [OR]: 0.963, 95% confidence interval [CI]: 0.960-0.965 and OR: 0.986, 95% CI: 0.984-0.988, respectively). A consistent result was found among school districts in all 4 regions and each poverty level.

Conclusions: Hybrid may not necessarily be the next logical option when transitioning from the remote to in-person learning models due to its consistent higher case growth rates than the other 2 learning models.
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http://dx.doi.org/10.1111/josh.13009DOI Listing
May 2021

Decreased Plasma MANF Levels are Associated with Type 2 Diabetes.

Biomed Environ Sci 2021 Mar;34(3):236-240

Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.

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http://dx.doi.org/10.3967/bes2021.030DOI Listing
March 2021

Sex-based differences in and risk factors for metabolic syndrome in adults aged 40 years and above in Northeast China: Results from the cross-sectional China national stroke screening survey.

BMJ Open 2021 Mar 24;11(3):e038671. Epub 2021 Mar 24.

Stroke Center, Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, China

Objectives: Low levels of income and education are risk factors for metabolic syndrome in the population of Northeast China, which has a high incidence of metabolic syndrome and cardiovascular diseases. This study aimed to determine sex-based differences associated with the prevalence of and risk factors for metabolic syndrome among people older than 40 years in Northeast China; this has not been previously investigated.

Design: This study analysed a portion of the large sample data of the national cross-sectional screening of China from 2016. Metabolic syndrome was defined as the presence of any three of the following five risk factors: abnormal waist circumference; high levels of triglycerides, high-density lipoprotein cholesterol or fasting plasma glucose; and elevated blood pressure. Multiple regression analysis was used to investigate sex-based differences in the prevalence of, and risk factors for metabolic syndrome.

Setting: The study was conducted in Dehui City, Jilin Province, China.

Participants: A total of 4052 participants with complete questionnaire information and laboratory examination results were included.

Results: The prevalence of metabolic syndrome was 50.1% overall (38.4% in men and 57.9% in women; p0.001). High body mass index and hip circumference were associated with metabolic syndrome in both sexes. In addition, physical inactivity (OR and 95% CI 1.44 (1.06 to 1.97); p0.022) in men and advanced age (OR and 95% CI 1.54 (1.15 to 2.04); p=0.003) in women were factors associated with metabolic syndrome. Women with junior high school education or above and living in rural areas were less likely to have metabolic syndrome. For men, education and rural or urban living had no association with metabolic syndrome.

Conclusions: The risk factors for metabolic syndrome have similarities and differences in different sexes; thus, the prevention and treatment of metabolic syndrome should be based on these sex differences.
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http://dx.doi.org/10.1136/bmjopen-2020-038671DOI Listing
March 2021

A robotic chewing simulator supplying six-axis mandibular motion, high occlusal force, and a saliva environment for denture tests.

Proc Inst Mech Eng H 2021 Mar 24:9544119211005601. Epub 2021 Mar 24.

College of Stomatology, Dalian Medical University, Dalian, China.

Six-axis motion is essential for the evaluation of the wear failure modes of dental prostheses with complete teeth morphologies, and a high occlusal force capacity is vital for static clenching and dynamic bruxism. Additionally, the saliva environment influences abrasive particles and crack growth. The present research was aimed at the development of a six-axis masticatory and saliva simulator with these capacities. The masticatory simulator was designed based on a six-axis parallel mechanism, and the saliva simulator consisted of a saliva circuit and a temperature control loop. A control system of the masticatory and saliva simulators was constructed. The operating interface includes a centric occlusal position search, a static test, a dynamic test, a saliva supply, and data reporting. The motion and force performances of the masticatory simulator were evaluated. The flow rate and temperature change of the saliva simulator were calculated. For the occlusal position-searching, the driving amplitude is linear with the moving variables during minor one-axis motion. For the static tests, the force capacity of the driving chain is 3540 N, while for the dynamic tests, the force capacity is 1390 N. The flow rate of the saliva is 0.18-51.84 mL/min, and the saliva can effectively wet the prosthesis without the risk of overflow. Moreover, the saliva temperature can increase from room temperature (23°C) to body temperature (37°C) in about 6 min. The proposed DUT-2 simulator with six-axis motion, high force, and a salvia environment provides an in vitro testing approach to validate numerical simulation results and explain the clinical failure modes of prostheses. The centric occlusal position-searching, static tests, and dynamic tests could therefore be executed using a single testing machine. Moreover, the proposed device is more compact than previously reported six-axis masticatory simulators, including the Bristol simulator and DUT-1 simulator.
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http://dx.doi.org/10.1177/09544119211005601DOI Listing
March 2021

High contrast ultrasonic method with multi-spatiotemporal compounding for monitoring catheter-based ultrasound thermal therapy: Development and Ex Vivo Evaluations.

IEEE Trans Biomed Eng 2021 Mar 23;PP. Epub 2021 Mar 23.

Objective: Changes in ultrasound backscatter energy (CBE) imaging can monitor thermal therapy. Catheter-based ultrasound (CBUS) can treat deep tumors with precise spatial control of energy deposition and ablation zones, of which CBE estimation can be limited by low contrast and robustness due to small or inconsistent changes in ultrasound data. This study develops a multi-spatiotemporal compounding CBE (MST-CBE) imaging approach for monitoring specific to CBUS thermal therapy.

Methods: Ex vivo thermal ablations were performed with stereotactic positioning of a 180 directional CBUS applicator, temperature monitoring probes, endorectal US probe, and subsequent lesion sectioning and measurement. Five frames of raw radiofrequency data were acquired throughout in 15s intervals. Using window-by-window estimation methods, absolute and positive components of MST-CBE images at each point were obtained by the compounding ratio of squared envelope data within an increasing spatial size in each short-time window.

Results: Compared with conventional US, Nakagami, and CBE imaging, the detection contrast and robustness quantified by tissue-modification-ratio improved by 37.24.7 (p<0.001), 37.55.2 (p<0.001), and 6.44.0 dB (p<0.05) in the MST-CBE imaging, respectively. Correlation coefficient and bias between cross-sectional dimensions of the ablation zones measured in tissue sections and estimated from MST-CBE were up to 0.91 (p<0.001) and -0.02 mm2, respectively.

Conclusion: The MST-CBE approach can monitor the detailed changes within target tissues and effectively characterize the dimensions of the ablation zone during CBUS energy deposition.

Significance: The MST-CBE approach could be practical for improved accuracy and contrast of monitoring and evaluation for CBUS thermal therapy.
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http://dx.doi.org/10.1109/TBME.2021.3067910DOI Listing
March 2021

Synthesis and Characterization of a Multifunctional Sustained-Release Organic-Inorganic Hybrid Microcapsule with Self-Healing and Flame-Retardancy Properties.

ACS Appl Mater Interfaces 2021 Apr 23;13(13):15668-15679. Epub 2021 Mar 23.

State Key Laboratory of Coal Mine Safety Technology, Shenyang Research Institute, China Coal Technology and Engineering Group, Fushun 113122, China.

As their service life increases, cement-based materials inevitably undergo microcracking and local damage. In response to this problem, this study used phacoemulsification-solvent volatilization to prepare a multifunctional sustained-release microcapsule (SFRM) with self-healing and flame-retardant characteristics. The synthesis of SFRM is based on the modification of ethyl cellulose with nano-SiO particles and cross-linking with a silane coupling agent to form an organic-inorganic hybrid wall material. The epoxy resin is blended with hexaphenoxy cyclotriphosphazene (HPCTP) to form a composite core emulsion. The surface morphology, particle size distribution, core-shell composition, and thermal stability of SFRM were analyzed via scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), Malvern, Fourier-transform infrared (FT-IR), and TD-DSC-DTG. It is concluded that SFRM was successfully synthesized with superior particle size distribution and thermal stability. When the ratio of SiO solution and EC alcohol solution reached 1:2, the particle size distribution of the microcapsules was 30-190 μm, and the decreased to 70 μm. The core material content, slow-release performance, and flame retardancy of SFRM were measured using a UV-1800 spectrophotometer and Hartmann tubes, and the compressive and repair properties of SFRM were evaluated by uniaxial compression tests. The results demonstrate that SFRM has satisfactory slow-release and flame-retardancy properties, the LC is 67%, and the first-order kinetic model shows the best fit and conforms to the non-Fickian diffusion mechanism. The SFRM repair rate can reach approximately 61%. This is of substantial significance to the field of self-repairing cement-based materials.
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http://dx.doi.org/10.1021/acsami.1c01540DOI Listing
April 2021

Wearable and Implantable Intraocular Pressure Biosensors: Recent Progress and Future Prospects.

Adv Sci (Weinh) 2021 Mar 21;8(6):2002971. Epub 2021 Jan 21.

State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology The First Affiliated Hospital of Sun Yat-Sen University Sun Yat-Sen University Guangzhou 510006 China.

Biosensors worn on or implanted in eyes have been garnering substantial attention since being proven to be an effective means to acquire critical biomarkers for monitoring the states of ophthalmic disease, diabetes. Among these disorders, glaucoma, the second leading cause of blindness globally, usually results in irreversible blindness. Continuous intraocular pressure (IOP) monitoring is considered as an effective measure, which provides a comprehensive view of IOP changes that is beyond reach for the "snapshots" measurements by clinical tonometry. However, to satisfy the applications in ophthalmology, the development of IOP sensors are required to be prepared with biocompatible, miniature, transparent, wireless and battery-free features, which are still challenging with many current fabrication processes. In this work, the recent advances in this field are reviewed by categorizing these devices into wearable and implantable IOP sensors. The materials and structures exploited for engineering these IOP devices are presented. Additionally, their working principle, performance, and the potential risk that materials and device architectures may pose to ocular tissue are discussed. This review should be valuable for preferable structure design, device fabrication, performance optimization, and reducing potential risk of these devices. It is significant for the development of future practical IOP sensors.
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http://dx.doi.org/10.1002/advs.202002971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967055PMC
March 2021

In Situ Growth of GeS Nanowires with Sulfur-Rich Shell for Featured Negative Photoconductivity.

J Phys Chem Lett 2021 Apr 19;12(12):3046-3052. Epub 2021 Mar 19.

School of Physics, School of Microelectronics, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.

The negative photoconductivity (NPC) effect originating from the surface shell layer has been considered as an efficient approach to improve the performance of optoelectronic nanodevices. However, a scientific design and precise growth of NPC-effect-caused shell during nanowire (NW) growth process for achieving high-performance photodetectors are still lacking. In this work, GeS NWs with a controlled sulfur-rich shell, diameter, and length are successfully prepared by a simple chemical vapor deposition method. As checked by transmission electron microscopy, the thickness of the sulfur-rich shell ranges from 10.5 ± 1.5 to 13.4 ± 2.5 nm by controlling the NW growth time. The composition of the sulfur-rich shell is studied by X-ray photoelectron spectroscopy, showing the decrease of S in the GeS shell from the surface to core. When configured into the well-known phototransistor, a featured NPC effect is observed, benefiting the high-performance photodetector with high responsivity of 10 A·W and detectivity of 10 Jones for λ = 405 nm with ultralow intensity of 0.04 mW·cm. However, the thicker-shell NW phototransistor shows an unstable photodetector behavior with smaller negative photocurrent because of more hole-trapping states in the thicker shell. All results suggest a careful design and controlled growth of an NPC-effect-caused shell for future optoelectronic applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c00540DOI Listing
April 2021

Multi-Phase Heterostructure of CoNiP/Co P for Enhanced Hydrogen Evolution Under Alkaline and Seawater Conditions by Promoting H O Dissociation.

Small 2021 Mar 18:e2007557. Epub 2021 Mar 18.

Institute of Applied Physics and Materials Engineering, University of Macau, Macao, Macao SAR, 999078, China.

Hydrogen evolution reaction (HER) is a key step for electrochemical energy conversion and storage. Developing well defined nanostructures as noble-metal-free electrocatalysts for HER is promising for the application of hydrogen technology. Herein, it is reported that 3D porous hierarchical CoNiP/Co P multi-phase heterostructure on Ni foam via an electrodeposition method followed by phosphorization exhibits ultra-highly catalytic activity for HER. The optimized CoNiP/Co P multi-phase heterostructure achieves an excellent HER performance with an ultralow overpotential of 36 mV at 10 mA cm , superior to commercial Pt/C. Importantly, the multi-phase heterostructure shows exceptional stability as confirmed by the long-term potential cycles (30,000 cycles) and extended electrocatalysis (up to 500 h) in alkaline solution and natural seawater. Experimental characterizations and DFT calculations demonstrate that the strong electronic interaction at the heterointerface of CoNiP/CoP is achieved via the electron transfer from CoNiP to the heterointerface, which directly promotes the dissociation of water at heterointerface and desorption of hydrogen on CoNiP. These findings may provide deep understanding on the HER mechanism of heterostructure electrocatalysts and guidance on the design of earth-abundant, cost-effective electrocatalysts with superior HER activity for practical applications.
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http://dx.doi.org/10.1002/smll.202007557DOI Listing
March 2021

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Cell Death Differ 2021 Mar 17. Epub 2021 Mar 17.

Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

It is well established that ferroptosis is primarily induced by peroxidation of long-chain poly-unsaturated fatty acid (PUFA) through nonenzymatic oxidation by free radicals or enzymatic stimulation of lipoxygenase. Although there is emerging evidence that long-chain saturated fatty acid (SFA) might be implicated in ferroptosis, it remains unclear whether and how SFA participates in the process of ferroptosis. Using endogenous metabolites and genome-wide CRISPR screening, we have identified FAR1 as a critical factor for SFA-mediated ferroptosis. FAR1 catalyzes the reduction of C16 or C18 saturated fatty acid to fatty alcohol, which is required for the synthesis of alkyl-ether lipids and plasmalogens. Inactivation of FAR1 diminishes SFA-dependent ferroptosis. Furthermore, FAR1-mediated ferroptosis is dependent on peroxisome-driven ether phospholipid biosynthesis. Strikingly, TMEM189, a newly identified gene which introduces vinyl-ether double bond into alkyl-ether lipids to generate plasmalogens abrogates FAR1-alkyl-ether lipids axis induced ferroptosis. Our study reveals a new FAR1-ether lipids-TMEM189 axis dependent ferroptosis pathway and suggests TMEM189 as a promising druggable target for anticancer therapy.
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http://dx.doi.org/10.1038/s41418-021-00769-0DOI Listing
March 2021

Puerarin Attenuates LPS-Induced Inflammatory Responses and Oxidative Stress Injury in Human Umbilical Vein Endothelial Cells through Mitochondrial Quality Control.

Oxid Med Cell Longev 2021 27;2021:6659240. Epub 2021 Feb 27.

State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

Atherosclerosis is closely associated with the inflammatory reaction of vascular endothelial cells. Puerarin (Pue), the main active component isolated from the rhizome of , is an isoflavone compound with potent antioxidant properties. Although Pue exhibits promising antiatherosclerotic pharmacological effects, only a few studies have reported its protective effect on endothelial cells. This study found that Pue could partly regulate mitochondrial function in human umbilical vein endothelial cells (HUVECs) and reduce or inhibit lipopolysaccharide-induced inflammatory reactions and oxidative stress injury in HUVECs, likely via mitochondrial quality control. Furthermore, the protective effect of Pue on HUVECs was closely related to the SIRT-1 signaling pathway. Pue increased autophagy and mitochondrial antioxidant potential via increased SIRT-1 expression, reducing excessive production of ROS and inhibiting the expression of inflammatory factors and oxidative stress injury. Therefore, Pue may improve mitochondrial respiratory function and energy metabolism, increasing the vulnerability of HUVECs to an inflammatory state.
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http://dx.doi.org/10.1155/2021/6659240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937474PMC
February 2021

Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity.

Nucleic Acids Res 2021 Mar 15. Epub 2021 Mar 15.

State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200438, China.

The compact CRISPR/Cas9 system, which can be delivered with their gRNA and a full-length promoter for expression by a single adeno-associated virus (AAV), is a promising platform for therapeutic applications. We previously identified a compact SauriCas9 that displays high activity and requires a simple NNGG PAM, but the specificity is moderate. Here, we identified three compact Cas9 orthologs, Staphylococcus lugdunensis Cas9 (SlugCas9), Staphylococcus lutrae Cas9 (SlutrCas9) and Staphylococcus haemolyticus Cas9 (ShaCas9), for mammalian genome editing. Of these three Cas9 orthologs, SlugCas9 recognizes a simple NNGG PAM and displays comparable activity to SaCas9. Importantly, we generated a SlugCas9-SaCas9 chimeric nuclease, which has both high specificity and high activity. We finally engineered SlugCas9 with mutations to generate a high-fidelity variant that maintains high specificity without compromising on-target editing efficiency. Our study offers important minimal Cas9 tools that are ideal for both basic research and clinical applications.
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http://dx.doi.org/10.1093/nar/gkab148DOI Listing
March 2021

Characterization of PMI-5011 on the Regulation of Deubiquitinating Enzyme Activity in Multiple Myeloma Cell Extracts.

Biochem Eng J 2021 Feb 4;166. Epub 2020 Nov 4.

Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, 70803.

Deubiquitinating enzyme (DUB)-targeted therapeutics have shown promise in recent years as alternative cancer therapeutics, especially when coupled with proteasome-based inhibitors. While a majority of DUB-based therapeutics function by inhibiting DUB enzymes, studies show that positive regulation of these enzymes can stabilize levels of protein degradation. Unfortunately, there are currently no clinically available therapeutics for this purpose. The goal of this work was to understand the effect of a botanical extract from called PMI-5011 on DUB activity in cancer cells. Through a series of kinetic analyses and mathematical modeling, it was found that PMI-5011 positively regulated DUB activity in two model multiple myeloma cells line (OPM2 and MM.1S). This suggests that PMI-5011 interacts with the active domains of DUBs to enhance their activity directly or indirectly, without apparently affecting cellular viability. Similar kinetic profiles of DUB activity were observed with three bioactive compounds in PMI-5011 (DMC-1, DMC-2, davidigenin). Interestingly, a differential cell line-independent trend was observed at higher concentrations which suggested variances in inherent gene expressions of UCHL1, UCHL5, USP7, USP15, USP14, and Rpn11 in OPM2 and MM.1S cell lines. These findings highlight the therapeutic potential of PMI-5011 and its selected bioactive compounds in cancer.
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http://dx.doi.org/10.1016/j.bej.2020.107834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949241PMC
February 2021

Potential mechanisms underlying the protective effects of Tricholoma matsutake singer peptides against LPS-induced inflammation in RAW264.7 macrophages.

Food Chem 2021 Mar 3;353:129452. Epub 2021 Mar 3.

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

This study aimed to investigate the protective effects of a < 3 kDa Tricholoma matsutake Singer peptide (TMWP) on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages. The results showed that TMWP significantly upregulated superoxide dismutase (SOD) activity and reduced reactive oxygen species (ROS) generation in RAW264.7 macrophages. Western blotting and immunofluorescence analysis indicated that TMWP inhibited the activation of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways, thereby reducing the secretion of IL-1β and IL-6 and the expression of TNF-α, COX-2, and iNOS. Additionally, TMWP improved mitochondrial respiration in LPS-stressed macrophages, counteracting the harmful effects of LPS treatment on mitochondrial function. Three peptides (SDIKHFPF, SDLKHFPF, and WFNNAGP) with the highest predicted scores for potential anti-inflammatory activity were identified using nano-HPLC-MS/MS. These data indicated that T. matsutake peptides could be an attractive natural ingredient for developing novel functional foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.129452DOI Listing
March 2021

Digestive Tract Injuries Caused by Ingested Foreign Bodies Containing Magnets.

Indian Pediatr 2021 Mar;58(3):280-281

Department of Pathology, School of Basic Medical Sciences, Ningxia Medical University; Yinchuan, China.

We report on 16 children with ingestion of magnetic foreign bodies, who were identified by a medical record review of our hospital data for the time period between January, 2017 and May, 2018. Digestive tract wall was sandwiched in 13 (75%) children and 11 (74%) had gaptic intestinal perforation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005281PMC
March 2021

Root Developmental Responses to Phosphorus Nutrition.

Authors:
Dong Liu

J Integr Plant Biol 2021 Mar 12. Epub 2021 Mar 12.

MOE Key laboratory of Bioinformatics, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Phosphorus is an essential macronutrient for plant growth and development. Root system architecture (RSA) affects a plant's ability to obtain phosphate, the major form of phosphorus that plants uptake. In this review, I first consider the relationship between RSA and plant phosphorus-acquisition efficiency, describe how external phosphorus conditions both induce and impose changes in the RSA of major crops and of the model plant Arabidopsis, and discuss whether shoot phosphorus status affects RSA and whether there is a universal root developmental response across all plant species. I then summarize the current understanding of the molecular mechanisms governing root developmental responses to phosphorus deficiency. I also explore the possible reasons for the inconsistent results reported by different research groups and comment on the relevance of some studies performed under laboratory conditions to what occurs in natural environments. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13090DOI Listing
March 2021

Dissipative Floquet Majorana Modes in Proximity-Induced Topological Superconductors.

Phys Rev Lett 2021 Feb;126(8):086801

State Key Laboratory of Low Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China.

We study a realistic Floquet topological superconductor, a periodically driven nanowire proximitized to an equilibrium s-wave superconductor. Because of the strong energy and density fluctuations caused by the superconducting proximity effect, the Floquet Majorana wire becomes dissipative. We show that the Floquet band structure is still preserved in this dissipative system. In particular, we find that the Floquet Majorana zero and π modes can no longer be simply described by the Floquet topological band theory. We also propose an effective model to simplify the calculation of the lifetime of these Floquet Majoranas and find that the lifetime can be engineered by the external driving field.
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http://dx.doi.org/10.1103/PhysRevLett.126.086801DOI Listing
February 2021

Defect-engineered three-dimensional vanadium diselenide microflowers/nanosheets on carbon cloth by chemical vapor deposition for high-performance hydrogen evolution reaction.

Nanotechnology 2021 Apr 13;32(26). Epub 2021 Apr 13.

School of Physics, School of Microelectronics, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, People's Republic of China.

In the past decades, defect engineering has become an effective strategy to significantly improve the hydrogen evolution reaction (HER) efficiency of electrocatalysts. In this work, a facile chemical vapor deposition (CVD) method is firstly adopted to demonstrate defect engineering in high-efficiency HER electrocatalysts of vanadium diselenide nanostructures. For practical applications, the conductive substrate of carbon cloth (CC) is selected as the growth substrate. By using a four-time CVD method, uniform three-dimensional microflowers with defect-rich small nanosheets on the surface are prepared directly on the CC substrate, displaying a stable HER performance with a low Tafel slope value of 125 mV decand low overpotential voltage of 295 mV at a current density of 10 mA cmin alkaline electrolyte. Based on the results of x-ray photoelectron spectra and density functional theory calculations, the impressive HER performance originates from the Se vacancy-related active sites of small nanosheets, while the microflower/nanosheet homoepitaxy structure facilitates the carrier flow between the active sites and conductive substrate. All the results present a new route to achieve defect engineering using the facile CVD technique, and pave a novel way to prepare high-activity layered electrocatalysts directly on a conductive substrate.
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http://dx.doi.org/10.1088/1361-6528/abecb8DOI Listing
April 2021

Microwave-assisted synthesis of fluorescent silicon quantum dots for ratiometric sensing of Hg (II) based on the regulation of energy transfer.

Talanta 2021 May 11;226:122093. Epub 2021 Jan 11.

Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, 212013, China. Electronic address:

The rapid and sensitive detection of Hg is highly required to protect the environmental safety and human healthy. In the present work, a ratiometric fluorescent sensing platform, consisting of silicon quantum dots (SiQDs), Rox-labelled DNA (Rox-DNA), and Exonuclease III (Exo III), is developed for the accurate detection of Hg. As for fluorescent probe, we report the first use of glutathione as reduction reagent for the microwave synthesis of SiQDs, achieving the facile (using a house-hold microwave oven) and rapid (within 8 min) synthesis. Such SiQDs show pH-independent spectra and reversible fluorescent behavior with temperature. Moreover, experimental results revealed that the electrostatic interaction-induced aggregation of Rox-DNA and SiQDs facilitated the occurring of energy transfer (ET). And detection principle based on the regulation of ET between Rox and SiQDs with Exo III was designed for analysis. ET effect resulted in the fluorescent fading of Rox while that of SiQDs kept stable. For analysis, the addition of Hg led to the formation of double-stranded Rox-DNA via T-Hg-T. Exo III would cut these double-stranded DNA to release Rox and Hg, thereby impeding the ET effect and recovering the fluorescent of Rox. Such SiQDs/Rox-DNA/Exo III ratiometric fluorescent sensing platform exhibited a linear response concentration range of 0.02 nM-10 nM with a detection limit of 0.01 nM. It was successfully used to analyze the water and soil samples. The reliability was validated by ICP-MS. Our work should promote the practical application of ratiometric fluorescent assay.
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http://dx.doi.org/10.1016/j.talanta.2021.122093DOI Listing
May 2021

A Retrospective Study of Lung Transplantation in Patients With Lymphangioleiomyomatosis: Challenges and Outcomes.

Front Med (Lausanne) 2021 16;8:584826. Epub 2021 Feb 16.

Wuxi Lung Transplant Center, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.

Lymphangioleiomyomatosis (LAM) is a rare systemic disease that generally leads to a progressive decline in pulmonary function. Experience, especially from the Asian population, including combined drug therapy before and after lung transplantation (LT) in LAM, is still limited. This study aimed to summarize the clinical data from patients with pulmonary LAM who underwent LT at centers in China. A retrospective review of all patients with LAM undergoing LT at the two largest centers in China between 2010 and 2018 was conducted. Pre- and posttransplant data were assessed and analyzed. Overall, 25 patients with LAM underwent bilateral LT. The mean age was 35.0 ± 8.6 years at diagnosis and 36.8 ± 9.3 years at the time of transplant. Before LT, only six patients could complete pulmonary function test; the reachable mean forced expiratory volume in one second (FEV) before LT was 15.9 ± 6.9%. Twenty-one patients (84%) had a recurrent pneumothorax, four (16.0%) of which required pleurodesis. Eight patients (32%) were treated with sirolimus pretransplant for 3.9 years (1-9 years). The average intra-surgery bleeding volume was 1,280 ± 730 ml in need of a transfusion of 1,316 ± 874 ml due to moderate-to-severe adhesion and pretransplant pleurodesis. The causes of death of four patients (16%) included primary graft dysfunction, bronchial dehiscence with long-term use of sirolimus, and uncontrollable infections. The median follow-up time from LT was 41.1 ± 25.0 months. LT for LAM patients from the Asian population has been reinforced from the data that we presented. Peri-transplantation use of sirolimus and LAM-related complications should be further defined and under constant surveillance.
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http://dx.doi.org/10.3389/fmed.2021.584826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924661PMC
February 2021