Publications by authors named "Dong Li"

2,843 Publications

  • Page 1 of 1

A Yeast BiFC-seq Method for Genome-wide Interactome Mapping.

Genomics Proteomics Bioinformatics 2021 Jul 24. Epub 2021 Jul 24.

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China. Electronic address:

Genome-wide physical protein-protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we report a high-efficiency yeast bimolecular fluorescence complementation method coupled with next-generation DNA sequencing (BiFC-seq) for interactome mapping. We applied this technique to systematically investigate an intraviral network of the Ebola virus (EBOV). Two-thirds (9/13) of known interactions of EBOV were recaptured, and five novel interactions were discovered. Next, we used the BiFC-seq method to map the interactome of the tumor protein p53. We identified 97 interactors of p53, more than three-quarters of which were novel. Furthermore, in a more complex background, we screened potential interactors by pooling two BiFC libraries together and revealed a network of 229 interactions among 205 proteins. These results show that BiFC-seq is a highly sensitive, rapid, and economical method for genome-wide interactome mapping.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gpb.2021.02.008DOI Listing
July 2021

Rapidly Thermoreversible and Biodegradable Polypeptide Hydrogels with Sol-Gel-Sol Transition Dependent on Subtle Manipulation of Side Groups.

Biomacromolecules 2021 Jul 23. Epub 2021 Jul 23.

CAS Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.

Thermoreversible hydrogels are attractive materials for biomedical applications, but their applications are still limited by nonbiodegradability and/or slow temperature-dependent gel-to-sol transition rates. In this research, we prepared a range of amphiphilic diblock, triblock, and four-armed star block copolymers composed of poly(ethylene glycol) (PEG) and poly(γ-(2-(2-ethoxyethoxy)ethyl)-l-glutamate) (P(EEOLG)) segments, which can form rapidly thermoreversible hydrogels at physiological temperature. Intriguingly, the obtained hydrogels can transform from gel to sol within 10-70 s in response to the temperature decrease from 37 to 0 °C. The thermosensitive sol-gel-sol transitions are markedly faster than previously reported thermoreversible PEG-poly(l-glutamate) derivative hydrogels with subtle differences in the side groups and a widely studied poly(d,l-lactide--glycolide)--PEG--poly(d,l-lactide--glycolide) (PLGA-PEG-PLGA) hydrogel that required a much longer time of 40∼150 min. Further investigation of the relationship between the hydrogel property and polymer structure is performed, and the self-assembly mechanisms of different copolymers are proposed. Cytotoxicity assays and subcutaneous degradation experiments reveal that the PEG/P(EEOLG) block copolymers are biocompatible and biodegradable. The polypeptide hydrogel can therefore be used as a three-dimensional platform for facile cell culture and collection by regulating the temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biomac.1c00583DOI Listing
July 2021

Chiral enantiomers of the plant growth regulator paclobutrazol selectively affect community structure and diversity of soil microorganisms.

Sci Total Environ 2021 Jul 16;797:148942. Epub 2021 Jul 16.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Paclobutrazol is a triazole plant growth regulator with a wide range of applications in crop and fruit tree production. Paclobutrazol is used as a racemic mixture in agriculture. However, the effects of paclobutrazol enantiomers on soil microbial community structure and diversity are unclear. In the present study, Illumina high-throughput sequencing was used to study the enantioselective effects of two paclobutrazol enantiomers on soil microbial community. S-paclobutrazol was more persistent than R-paclobutrazol. The half-lives of the S- and R-isomers were 80 d and 50 d, respectively. No interconversion between the two isomers occurred in soils. In addition, the enantiomers had significant enantiomeric effects on soil microbial community and the paclobutrazol degradation was probably attributed to the presence of Pseudomonas and Mycobacterium. Notably, the relative abundance of Fusarium, a genus of filamentous fungi producing gibberellins, could be enantioselectively affected by the chiral enantiomers. Paclobutrazol enantiomers exhibited greater effects on the fungal community structure than bacterial community structure due to the fungicidal activity of paclobutrazol. Finally, R-paclobutrazol had a significant effect on the microbial networks. The findings of the present study suggest that the use of S-paclobutrazol may accomplish both plant growth regulation and the minimization of effects of paclobutrazol on soil microbial communities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148942DOI Listing
July 2021

[Effects of Different Nitrite Generation on the Short-cut Nitrification Denitrifying Phosphorus Removal Granules System].

Huan Jing Ke Xue 2021 Aug;42(8):3858-3865

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.

To explore the effects of nitrite generation on the system of short-cut nitrification denitrifying phosphorus removal granules, nitrite was produced continuously and intermittently, under continuous and intermittent aeration, in two groups of SBR reactors of the same size. The effects of nitrogen and phosphorus removal, physical characteristics of the sludge, and microbial community structure were investigated. Nitrite was consumed immediately after intermittent production, with better and more stable nitrogen and phosphorus removal performance. In particular, the average rate of TN removal was 92.07% after 72 days. The utilization efficiency of the carbon source (by P/COD) was concentrated at 0.21-0.22 mg ·mg, to ensure full utilization of the carbon source and to further promote denitrification and phosphorus removal. Particle sizes were uniform and showed concentrated distribution, with particles exhibiting regular shapes and clear boundaries. Microbial community analysis showed that the abundance and diversity of microbial communities were higher in the intermittent nitrite system and more enriched in DPAOs genera ( and ). The combination of DPAOs genera and resulted in a dynamic balance and stable operation of the short-cut nitrification and denitrifying phosphorus removal system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202101242DOI Listing
August 2021

Application of biomarkers in the diagnosis of uncertain samples of core needle biopsy of thyroid nodules.

Virchows Arch 2021 Jul 26. Epub 2021 Jul 26.

Department of Pathology, Peking University First Hospital, 7 Xishiku Street, Xicheng District, Beijing, 100034, China.

Core needle biopsy (CNB) is now more frequently used for the preoperative diagnosis of thyroid nodules. Based on morphology alone, 5-20% of CNB samples cannot be determined as malignant or benign. Compared to fine-needle biopsy (FNB), samples collected by CNB are more accessible for various tests. Therefore, studying biomarkers' application in distinguishing uncertain CNB samples of thyroid nodules is a practical need. Patients of thyroid nodules with both CNB and matched resected specimens were reviewed. Cases classified as indeterminate lesions, follicular neoplasms, and suspicious for malignancy were retrieved. All CNB samples were stained by immunohistochemistry (IHC) using antibodies against CK19, galectin-3, HBME-1, and CD56 and detected by next-generation sequencing (NGS) using an OncoAim® thyroid cancer multigene assay kit (Singlera Genomics) that detected 26 genes. Taking the resected specimens' classification as the gold standard, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy of a single biomarker, and various combinations for discriminating malignancy from benignity were calculated. The sensitivity, specificity, PPV, NPV, and accuracy for preoperative malignancy evaluation were as follows. In the cohort of non-follicular-neoplasm-lesions (non-FN-lesion), they were 95.16%, 53.85%, 90.77%, 70.00%, and 88.00% for CK19; 95.16%, 38.46%, 88.06%, 62.50%, and 85.33% for galectin-3; 77.42%, 76.92%, 94.12%, 41.67%, and 58.00% for HBME-1; 66.13%, 100.00%, 100.00%, 38.24%, and 72.00% for CD56; 90.32%, 92.31%, 98.25%, 66.67%, and 90.67% for NGS; and 88.71%, 92.30%, 98.21%, 63.16%, and 89.33% for integrated IHC. In the cohort of follicular neoplasms (FN), they were 30.43%, 77.77%, 77.77%, 30.43%, and 43.75% for CK19; 73.91%, 66.67%, 85.00%, 50.00%, and 71.88% for galectin-3; 26.09%, 88.89%, 85.71%, 32.00%, and 43.75% for HBME-1; 26.09%, 100.00%, 100.00%, 34.62%, and 46.88% for CD56; 52.17%, 88.89%, 92.31%, 42.11%, and 62.50% for NGS; 82.61%, 66.67%, 86.36%, 60.00%, and 78.13% for integrated IHC; and 100%, 66.67%, 88.46%, 100%, and 90.63% for integrated IHC-NGS. The application of biomarkers in distinguishing uncertain CNB samples of thyroid nodules is available and capable. CD56 negative or NGS positive suggests malignancy strongly for both FN and non-FN-lesion, which may be used as a "rule in" tool. The negative predictive value of the integrated IHC and the integrated IHC-NGS implies a high possibility to be benign for non-FN-lesion and FN separately, which can work as a "rule out" tool. Considering the balance of specificity and sensitivity, NGS is the best for non-FN-lesion and the integrated IHC-NGS is the best for FN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00428-021-03161-yDOI Listing
July 2021

-Related Mitochondrial Fission Defects Presenting as Encephalopathy: A Case Report and Literature Review.

Front Pediatr 2021 8;9:626657. Epub 2021 Jul 8.

Department of Pediatric Neurology, Tianjin Children's Hospital/Tianjin University Children's Hospital, Tianjin, China.

Mitochondrial dynamics, including mitochondrial fission and fusion, transport and distribution, biogenesis and degradation, are critical to neuronal function. The dynamin-1 like gene encodes dynamin-related protein 1 (DRP1/DLP1), which is an evolutionarily conserved member of the dynamin family and is responsible for mitochondrial division. variants can lead to mitochondrial fission dysfunction and neurological disorders. We report a case of -related mitochondrial disease admitted to Tianjin Children's Hospital. We searched for similar reported cases in the PubMed database using the terms "" and "mitochondrial," reviewed recent literature to summarize the clinical and genetic characteristics, and analyzed genotype-phenotype correlations. The patient presented with psychomotor retardation, motor disturbance (muscle weakness with paroxysmal hypermyotonia), and a variant (c.116G>A, g.22229G>A, p.S39N) in the GTPase domain of (reference sequence NM_012062), which has not previously been reported in the literature. This case was combined with an additional 35 cases identified in 20 relevant references in order to analyze a total of 36 patients. The male-to-female ratio was 1:1.06, and the median age of onset was 6 months (range, neonatal period to 9 years). The cardinal symptoms included psychomotor retardation in 77.8% (28/36), limb paralysis in 66.7% (18/27), dystonia in 82.8% (24/29), and epilepsy in 59.4% (19/32). The clinical manifestations of variants in the GTPase domain of DRP1 were milder than those identified in the middle domain. This case report describes a new variant of the gene, and summarizes previously reported cases. Furthermore, the clinical phenotype and the genotype of gene-associated mitochondrial disease was analyzed to improve the understanding of this disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2021.626657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295552PMC
July 2021

Sociodemographic Correlates of Human Papillomavirus Vaccine Uptake: Opportunistic and Catch-Up Vaccination in Norway.

Cancers (Basel) 2021 Jul 12;13(14). Epub 2021 Jul 12.

Department of Research, Cancer Registry of Norway, 0304 Oslo, Norway.

Achieving equity in human papillomavirus (HPV) vaccination has high priority. In this nationwide registry-based study, we aimed to investigate sociodemographic correlates of HPV vaccine uptake among women who were vaccinated opportunistically at their own initiative and cost during October 2006-June 2018, and among women who were vaccinated free-of-charge in a catch-up vaccination program during November 2016-June 2018. For 840,328 female residents born in Norway between 1975 and 1996, we retrieved HPV vaccination and sociodemographic data from national registries. We used separate models to analyze the sociodemographic correlates of the initiation and completion of HPV vaccination in opportunistic and catch-up vaccination settings. Overall initiation rate for opportunistic HPV vaccination was 2.2%. Uptake increased consistently with birth year, maternal education level, and household income. Having two immigrant parents or a mother working in a lower prestige occupation was strongly associated with low opportunistic vaccination uptake. Similar but weaker inequities were observed in catch-up HPV vaccination. Initiation rate during the first 20 months of the catch-up program was 46.2%. Completion rate was 72.1% and 73.0% for opportunistic or catch-up vaccination, respectively, with small inequities. In conclusion, HPV vaccine uptake was strongly associated with sociodemographic background both in opportunistic and catch-up vaccination settings, with particularly low uptake associated with having two immigrant parents and low household income.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers13143483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307029PMC
July 2021

The synergistic effect of rumen cellulolytic bacteria and activated carbon on thermophilic digestion of cornstalk.

Bioresour Technol 2021 Jul 16;338:125566. Epub 2021 Jul 16.

CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; College of Engineering, Northeast Agricultural University, No. 600, Changjiang Road, Xiangfang District, Harbin, Heilongjiang, 150030, PR China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

To explore the bioaugmentation of rumen cellulolytic bacteria (RCB) and activated carbon (AC) on thermophilic digestion of cornstalk, biochemical methane potential tests were carried out. Adding RCB or AC can improve methane production, while simultaneous existence of AC (10 g/L) and RCB (5%) obtained the best performance. The maximum cellulose degradation rate, methane production rate and methane yield were 66.92%, 32.2 L/(kgVS·d), and 144.9 L/kgVS, which increased by 30.23%, 51.17%, and 20.35% compared with control group. The cellulolytic and fermentative bacteria (Hydrogenispora), syntrophic acetate-oxidizing bacteria (norank_o_MBA03), and hydrogenotrophic Methanothermobacter were crucial for thermophilic digestion of cornstalk. The enhancement of AC was due to the enrichment of Hydrogenispora and Methanothermobacter, while RCB can increase the abundance of cellulolytic bacteria (Halocella and norank_o_M55-D21) and mixotrophic Methanosarcina. The synergetic effect of AC and RCB owing to the enriched cellulolytic bacteria, the enhanced syntrophic acetate oxidation and the concentrated carbon metabolic flow to methane.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.125566DOI Listing
July 2021

Cleft palate morphology, genetic etiology, and risk of mortality in infants with Robin sequence.

Am J Med Genet A 2021 Jul 22. Epub 2021 Jul 22.

Division of Craniofacial Medicine, Seattle Children's Hospital, Seattle, WA, USA.

Robin sequence (RS) has many genetic and nongenetic causes, including isolated Robin sequence (iRS), Stickler syndrome (SS), and other syndromes (SyndRS). The purpose of this study was to determine if the presence and type of cleft palate varies between etiologic groups. A secondary endpoint was to determine the relationship of etiologic group, cleft type, and mortality. Retrospective chart review of patients with RS at two high-volume craniofacial centers. 295 patients with RS identified. CP was identified in 97% with iRS, 95% with SS, and 70% of those with SyndRS (p < .0001). U-shaped CP was seen in 86% of iRS, 82% with SS, but only 27% with SyndRS (p < .0001). At one institution, 12 children (6%) with RS died, all from the SyndRS group (p < .0001). All died due to medical comorbidities related to their syndrome. Only 25% of children who died had a U-shaped CP. The most common palatal morphology among those who died was an intact palate. U-shaped CP was most strongly associated with iRS and SS, and with a lower risk of mortality. RS with submucous CP, cleft lip and palate or intact palate was strongly suggestive of an underlying genetic syndrome and higher risk of mortality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ajmg.a.62430DOI Listing
July 2021

Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth disease virus.

J Gen Virol 2021 Jul;102(7)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou 730046, PR China.

Pigs are susceptible to foot-and-mouth disease virus (FMDV), and the humoral immune response plays an essential role in protection against FMDV infection. However, little information is available about FMDV-specific mAbs derived from single B cells of pigs. This study aimed to determine the antigenic features of FMDV that are recognized by antibodies from pigs. Therefore, a panel of pig-derived mAbs against FMDV were developed using fluorescence-based single B cell antibody technology. Western blotting revealed that three of the antibodies (1C6, P2-7E and P2-8G) recognized conserved antigen epitopes on capsid protein VP2, and exhibited broad reactivity against both FMDV serotypes A and O. An alanine-substitution scanning assay and sequence conservation analysis elucidated that these porcine mAbs recognized two conserved epitopes on VP2: a linear epitope (KKTEETTLL) in the N terminus and a conformational epitope involving residues K63, H65, L66, F67, D68 and L81 on two β-sheets (B-sheet and C-sheet) that depended on the integrity of VP2. Random parings of heavy and light chains of the IgGs confirmed that the heavy chain is predominantly involved in binding to antigen. The light chain of porcine IgG contributes to the binding affinity toward an antigen and may function as a support platform for antibody stability. In summary, this study is the first to reveal the conserved antigenic profile of FMDV recognized by porcine B cells and provides a novel method for analysing the antibody response against FMDV in its natural hosts (i.e. pigs) at the clonal level.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/jgv.0.001608DOI Listing
July 2021

Palladium-Catalyzed Asymmetric Trifluoromethylated Allylic Alkylation of Pyrazolones Enabled by α-(Trifluoromethyl)alkenyl Acetates.

Org Lett 2021 Jul 19. Epub 2021 Jul 19.

State Key Laboratory of Fine Chemicals, Department of Pharmaceutical Sciences, School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, Dalian 116024, China.

The first asymmetric trifluoromethylated allylic alkylation of pyrazolones using α-(trifluoromethyl)alkenyl acetates as a novel trifluoromethylated allylation reagent is described, affording various functionalized chiral pyrazolones containing a trifluoromethylated allyl substituent in high yields with excellent regio-/enantio-/diastereoselectivities. Mechanistically, the double-bond migration of α-(trifluoromethyl)alkenyl acetates in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene is initial and interesting step. More importantly, this study is of significance in providing a novel and widely applicable trifluoromethyl-containing allylation reagent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c01957DOI Listing
July 2021

miR‑367‑3p downregulates Rab23 expression and inhibits Hedgehog signaling resulting in the inhibition of the proliferation, migration, and invasion of prostate cancer cells.

Oncol Rep 2021 Sep 19;46(3). Epub 2021 Jul 19.

Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China.

MicroRNAs play an important role in tumor cell proliferation, invasion, and Rab23 is a member of the Ras‑related small GTPase family and plays a critical role in the progression of may types of tumors. The present study was designed to investigate the inhibitory effect of microRNA (miR)‑367‑3p on the proliferation, invasion, and metastasis of prostate cancer cells. qRT‑PCR was used to detect the expression of miR‑367‑3p in prostate cancer and adjacent tissues. Cell proliferation, scratch, and Transwell assays were performed to verify the inhibitory effect of miR‑367‑3p overexpression or Ras‑related protein Rab 23 () knockdown on prostate cancer. Double luciferase reporter assay was utilized to verify whether miR‑367‑3p could target the Rab23 3'‑untranslated region (UTR). The expression levels of Rab23, Gli1, and Gli2 in prostate cancer cells transfected with the miR‑367‑3p mimic were detected via qRT‑PCR analysis. miR‑367‑3p expression in the prostate cancer tissues was downregulated compared with that in the para‑cancer control tissues. miR‑367‑3p expression in DU145 and PC3 cells was also downregulated compared with that in the human prostate epithelial cell line RWPE‑1. The overexpression of miR‑367‑3p or the knockdown of Rab23 inhibited the proliferation, invasion, and metastasis of prostate cancer cells. The results of the luciferase reporter assay confirmed that Rab23 was a target gene that was regulated by miR‑367‑3p. miR‑367‑3p specifically bound to the 3'‑UTR of Rab23 mRNA. The overexpression of miR‑367‑3p inhibited Rab23 expression and the Hedgehog pathway. Cell function experiments confirmed that the overexpression of Rab23 reversed the anticancer effect of miR‑367‑3p. miR‑367‑3p was able to inhibit the Hedgehog pathway by targeting the expression of the Rab23 gene, thus inhibiting the proliferation, invasion, and metastasis of prostate cancer cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/or.2021.8143DOI Listing
September 2021

Association Between Gamma-Glutamyl Transferase, Total Bilirubin and Systemic Lupus Erythematosus in Chinese Women.

Front Immunol 2021 29;12:682400. Epub 2021 Jun 29.

Department of Epidemiology and Health Statistics, School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, China.

Background: Systemic lupus erythematosus (SLE) affects many organs and systems of the human organism, at present, its specific pathogenesis is not completely clear, but inflammation is considered to be an important factor involved in the pathogenesis and progression of SLE. Gamma-glutamyl transpeptidase (GGT) and total bilirubin (TBIL) have different effects on inflammation: GGT has pro-inflammatory effects, on the contrary, TBIL has anti-inflammatory effects. Study has found that GGT and TBIL play opposite roles in metabolic diseases. However, the roles of them in SLE are unknown. Meanwhile, the relationship between GGT and SLE also remains unexplored.

Method: We recruited 341 SLE patients and 332 healthy individuals in Liaocheng People's Hospital from August 2018 to May 2019. We diagnosed SLE using 2019 revised American College of Rheumatology (ACR) SLE criteria, and modeled the study outcomes using logistic regression to explore the respective relationship between GGT, TBIL and SLE. We also analyzed the interaction of GGT and TBIL in the progression of SLE.

Results: We found that the levels of CRP, IL-6 and TNF-α in the aggravated group were significantly higher than those in the unaggravated group, the levels of C3 and C4 in the aggravated group were significantly lower than those in the unaggravated group. According to Spearman correlation analysis, GGT is proportional to CRP (r=0.417) and IL-6 (r=0.412), inversely proportional to C3 (r=-0.177) and C4 (r=0.-132). TBIL was inversely proportional to CRP (r=-0.328) and TNF(r=-0.360), and positively proportional to C3 (r=0.174) and C4 (r=0.172). In the fully adjusted model, compared to the lowest quartile, the highest quartile of GGT exhibited a positive association with the risk of SLE aggravation (OR=2.99, 95% CI: 1.42-6.31, <0.001). At the same time, compared to the highest quartile, the quartile lowest of TBIL exhibited a positive association with the risk of SLE aggravation (OR=2.66, 95% CI: 1.27-5.59, <0.001) in the fully adjusted model. Through interaction analysis, we found that women with high GGT levels had an increased risk of SLE aggravation when they had a low level of TBIL (OR=3.68, 95% CI: 1.51-9.01, for women with Q1 TBIL and Q4 GGT compared to women with Q2-Q4 TBIL and Q1-Q3 GGT, for interaction <0.001), the combined AUC value (AUC=0.711) of high GGT level and TBIL were higher than their respective values (AUC=0.612, AUC=0.614).

Conclusion: We found that the effects of GGT and TBIL in the progression of SLE are opposite. High GGT level might be a risk factor for SLE aggravation, as GGT levels increased, so did the risk of SLE aggravation. At the same time, we found that low TBIL level might be a risk factor for SLE aggravation. Moreover, high GGT level and low TBIL level had a subadditive effect on the increased risk of SLE aggravation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.682400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277571PMC
June 2021

A novel biliopancreatic duct endoscope combined optical coherence tomography with intraductal ultrasonography for exploring the bile duct: a diagnostic study in a porcine model.

Gastrointest Endosc 2021 Jul 15. Epub 2021 Jul 15.

Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China; Clinical Center and Key Lab of Intestinal and Colorectal Diseases of Hubei Province 430071, Wuhan, China. Electronic address:

Background And Aims: There are many deficiencies of existing biliopancreatic duct endoscopy in the examination of early biliary and pancreatic tumors. This study aimed to evaluate the usefulness and feasibility of a novel ultrafine separable biliopancreatic duct endoscopy device with dual modalities of intraductal ultrasonography (IDUS) and optical coherence tomography (OCT) for the in vivo assessment of biliopancreatic duct system during ERCP.

Methods: Five Bama miniature pigs were selected to probe their common bile duct and branches by using this novel equipment during ERCP. The feasibility of the procedure was evaluated by clear, clinically interpretable images obtained by using spiral scanning with pull-back method. The clinical usefulness of the novel product was evaluated by postoperative choledochoscopy and assessment of the animal's general condition.

Results: One hundred forty-one pairs of images from 5 Bama miniature pigs were acquired. Both OCT and IDUS's visualization of the bile duct was characterized by a differentiated 3-layer architecture, whereas IDUS shows with poor clarity than OCT. Postoperative choledochoscopy showed there was no local lesion in the bile duct wall, and the general condition of animals was normal.

Conclusions: This prospective evaluation indicates the feasibility and potential clinical value of the novel ultrafine separable biliopancreatic duct endoscopy device. The fusion of the 2 imaging modalities can shed light on the early diagnosis of biliary and pancreatic tumors. Further studies shall be carried out to establish the diagnosis criteria with the dual-modality imaging using animal pathological model and human clinic study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gie.2021.07.009DOI Listing
July 2021

Action Video Gaming Experience Related to Altered Resting-State EEG Temporal and Spatial Complexity.

Front Hum Neurosci 2021 29;15:640329. Epub 2021 Jun 29.

MOE Key Lab for Neuroinformation, The Clinical Hospital of Chengdu Brain Science Institute, University of Electronic Science and Technology of China, Chengdu, China.

Action video gaming (AVG) places sustained cognitive load on various behavioral systems, thus offering new insights into learning-related neural plasticity. This study aims to determine whether AVG experience is associated with resting-state electroencephalogram (rs-EEG) temporal and spatial complexity, and if so, whether this effect is observable across AVG subgenres. Two AVG games - League of Legends (LOL) and Player Unknown's Battle Grounds (PUBG) that represent two major AVG subgenres - were examined. We compared rs-EEG microstate and omega complexity between LOL experts and non-experts (Experiment 1) and between PUBG experts and non-experts (Experiment 2). We found that the experts and non-experts had different rs-EEG activities in both experiments, thus revealing the adaptive effect of AVG experience on brain development. Furthermore, we also found certain subgenre-specific complexity changes, supporting the recent proposal that AVG should be categorized based on the gaming mechanics of a specific game rather than a generic genre designation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2021.640329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275975PMC
June 2021

Validation of a Chin Retrusion Scale for Chinese Subjects.

J Craniofac Surg 2021 Jul 13. Epub 2021 Jul 13.

Peking University International Hospital Tsinghua University Affiliated Beijing Tsinghua Changgung Hospital Beijing Hospital Allergan Aesthetics, an AbbVie Company, Beijing, China Allergan Aesthetics, an AbbVie Company, Irvine, CA Beijing University Third Hospital, Beijing Shi, China.

Abstract: Chin augmentation procedures are gaining in popularity. The purpose of this study was to validate the China (Allergan) Chin Retrusion Scale (CACRS) and to evaluate the reliability of the scale. A team of 10 physicians based in Beijing, China, consisting of 1 principal investigator and 9 independent raters, assessed a pool of subject images. Using standardized equipment to capture 2-dimensional images, 100 subjects were photographed from a left, lateral, 90° view. Two measures were used to assess the validity of the scale: rater evaluations of clinically significant differences in 52 pairs of images and rater assessment of chin retrusion using the CACRS to grade the 100 images. The CACRS demonstrated almost perfect inter-rater agreement during 2 validation sessions, with intraclass correlation coefficients of 0.89 and 0.90 at sessions 1 and 2, respectively. Overall inter-rater agreement for all 9 raters was almost perfect, with a weighted kappa of 0.82. Based on inter- and intra-rater agreement, the CACRS was validated for physician ratings of chin retrusion in Chinese subjects. Results from clinical significance evaluations indicated that a 1-grade difference between image pairs was considered clinically meaningful for the CACRS. The CACRS is a validated and reliable photonumeric scale for the assessment of chin retrusion in Chinese men and women. The scale is a clinically significant reference tool for evaluating the effect of chin augmentation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SCS.0000000000007849DOI Listing
July 2021

circPUM1 Activates the PI3K/AKT Signaling Pathway by Sponging to Promote to Promote the Proliferation, Invasion and Glycolysis of Pancreatic Cancer.

Curr Pharm Biotechnol 2021 Jul 13. Epub 2021 Jul 13.

Department of General Surgery, The Seventh Affiliated Hospital, Sen Yat-sen University, ShenZhen, Guangdong 518017, China.

Background: Our study seeks to obtain data to assess the impacts of circPUM1 on pancreatic cancer (PC) and its mechanism.

Methods: The expression of circPUM1 and miR-200c-3p in PC and normal tissues and PC cell lines was collected and detected. Subsequently, dual-luciferase assay-based verification of the binding site of the two was carried out. After interfering with circPUM1 expression in MIAPaCa-2 and PANC-1 cells, cell proliferation, viability, apoptosis rate, invasion ability, glucose consumption, and lactate production were measured by MTT, colony formation, flow cytometry, Transwell assays, and glucose and lactate assay kits. Additionally, western blot was utilized for assessing PI3K/AKT signaling pathway-related proteins. From the results, highly expressed circPUM1 and miR-200c-3p in PC tissues and cells were proved.

Results: Down-regulation of circPUM1 expression significantly inhibited cell proliferation, cell viability, invasion, and glycolysis, while increasing the apoptosis rate. Down-regulated circPUM1 led to the inhibition of the PI3K/AKT signaling pathway activity in PC cells, while up-regulated circPUM1 increased its activity. Further experiments revealed that down-regulation of miR-200c-3p expression reversed the inhibitory effect of lowly expressed circPUM1 on PC cells.

Conclusion: In summary, circPUM1 activates PI3K/AKT signaling pathway by sponging miR-200c-3p and promotes PC progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1389201022666210713152457DOI Listing
July 2021

Scutellarin improves the radiosensitivity of non-small cell lung cancer cells to iodine-125 seeds via downregulating the AKT/mTOR pathway.

Thorac Cancer 2021 Jul 13. Epub 2021 Jul 13.

Department of Interventional Medicine, The Second Hospital of Shandong University, Jinan, China.

Background: In our previous study, we indicated that scutellarin (SCU) induced an anticancer effect in A549 cells. However, whether SCU regulates the radiosensitivity of non-small cell lung cancer (NSCLC) and its related mechanism is still unclear.

Methods: In this study, we explored the anticancer effect induced by iodine-125 ( I) and SCU at a sensitizing concentration in A549 and H1975 cells. Cellular apoptosis and proliferation were detected by flow cytometry, Bcl-2/Bax expression level, cell cycle, CCK-8, and EdU staining. A tumor model using nude mice was also carried out to investigate the combined effect of I and SCU in vivo. In addition, the expression level of AKT/mTOR pathway was detected to investigate whether it is linked to the anticancer effect of I and SCU.

Results: SCU at a sensitizing concentration promoted the I-induced apoptosis and antiproliferative effect in A549 and H1975 cells. Moreover, the same results were obtained in vivo. Based on our findings, the AKT/mTOR pathway was significantly downregulated after combined treatment with I and SCU.

Conclusions: The results of our study suggested that SCU promotes the anticancer effects induced by I in NSCLC cells by downregulating the AKT/mTOR pathway and lays a foundation for future application of this combined treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1759-7714.14077DOI Listing
July 2021

Effects of TRIM59 on RAW264.7 macrophage gene expression and function.

Immunobiology 2021 Jul 30;226(4):152109. Epub 2021 Jun 30.

Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province, China. Electronic address:

Macrophages have a variety of functions, such as secreting cytokines, phagocytosis, et al. Tripartite motif containing 59 (TRIM59) protein is highly expressed in tumor cells. It can regulate proliferation of tumor cells and promote tumor progression. Recent studies shown that the expression of TRIM59 was different in macrophages when stimulated by different stimuli, however, the effects of TRIM59 on macrophage gene expression profiles and functions are still unknown. In our study, we constructed RAW264.7 macrophages with high and low expression of TRIM59, and used next generation sequencing to explore the effects of TRIM59 on macrophage gene expression profiles. Results showed that TRIM59 affected an abundant number of genes, and may affect phagocytosis and cell cycles. We also examined the expression of surface molecules, secretion of cytokines, phagocytosis, proliferation, and apoptosis of macrophages, and confirmed that TRIM59 increased the expression of FcγRs CD16/32, CD64 and the secretion of TNF-α and IL-10, promoted phagocytosis and proliferation of RAW264.7 cells, inhibited the expression of complement receptor CD11b and antigen presentation related receptors (MHCII, CD80), but TRIM59 had no significant effect on apoptosis. Our study explored the effect of TRIM59 on the gene expression and function of macrophages comprehensively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.imbio.2021.152109DOI Listing
July 2021

THAN: task-driven hierarchical attention network for the diagnosis of mild cognitive impairment and Alzheimer's disease.

Quant Imaging Med Surg 2021 Jul;11(7):3338-3354

Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.

Background: To assist doctors to diagnose mild cognitive impairment (MCI) and Alzheimer's disease (AD) early and accurately, convolutional neural networks based on structural magnetic resonance imaging (sMRI) images have been developed and shown excellent performance. However, they are still limited in their capacity in extracting discriminative features because of large sMRI image volumes yet small lesion regions and the small number of sMRI images.

Methods: We proposed a task-driven hierarchical attention network (THAN) taking advantage of the merits of patch-based and attention-based convolutional neural networks for MCI and AD diagnosis. THAN consists of an information sub-network and a hierarchical attention sub-network. In the information sub-network, an information map extractor, a patch-assistant module, and a mutual-boosting loss function are designed to generate a task-driven information map, which automatically highlights disease-related regions and their importance for final classification. In the hierarchical attention sub-network, a visual attention module and a semantic attention module are devised based on the information map to extract discriminative features for disease diagnosis.

Results: Extensive experiments were conducted for four classification tasks: MCI versus () normal controls (NC), AD NC, AD MCI, and AD MCI NC. Results demonstrated that THAN attained the accuracy of 81.6% for MCI NC, 93.5% for AD NC, 80.8% for AD MCI, and 62.9% for AD MCI NC. It outperformed advanced attention-based and patch-based methods. Moreover, information maps generated by the information sub-network could highlight the potential biomarkers of MCI and AD, such as the hippocampus and ventricles. Furthermore, when the visual and semantic attention modules were combined, the performance of the four tasks was highly improved.

Conclusions: The information sub-network can automatically highlight the disease-related regions. The hierarchical attention sub-network can extract discriminative visual and semantic features. Through the two sub-networks, THAN fully exploits the visual and semantic features of disease-related regions and meanwhile considers global features of sMRI images, which finally facilitate the diagnosis of MCI and AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/qims-21-91DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249997PMC
July 2021

Bather Shedding as a Source of Human Fecal Markers to a Recreational Beach.

Front Microbiol 2021 25;12:673190. Epub 2021 Jun 25.

Bren School of Environmental Science and Management, University of California, Santa Barbara, Santa Barbara, CA, United States.

Microbial source tracking (MST) can identify and locate surf zone fecal indicator bacteria (FIB) sources. However, DNA-based fecal marker results may raise new questions, since FIB and DNA marker sources can differ. Here, during 2 years of summertime (dry season) MST for a Goleta, California recreational beach, surf zone FIB were mainly from gulls, yet low level human-associated DNA-based fecal marker (HF183) was detected in 25 and 14% of surf zone water samples, respectively. Watershed sources were hypothesized because dry weather creek waters had elevated FIB, and runoff-generating rain events mobilized human (and dog) fecal markers and spp. into creeks, with human marker HF183 detected in 40 and 50% of creek water samples, dog markers detected in 70 and 50% of samples, and spp. in 40 and 33.3% of samples, respectively over 2 years. However, the dry weather estuary outlet was bermed in the first study year; simultaneously, creek fecal markers and pathogens were lower or similar to surf zone results. Although the berm breached in the second year, surf zone fecal markers stayed low. Watershed sediments, intertidal beach sands, and nearshore sediments were devoid of HF183 and dog-associated DNA markers. Based on dye tests and groundwater sampling, beach sanitary sewers were not leaking; groundwater was also devoid of HF183. Offshore sources appeared unlikely, since FIB and fecal markers decreased along a spatial gradient from the surf zone toward nearshore and offshore ocean waters. Further, like other regional beaches, surf zone HF183 corresponded significantly to bather counts, especially in the afternoons when there were more swimmers. However, morning detections of surf zone HF183 when there were few swimmers raised the possibility that the wastewater treatment plant (WWTP) offshore outfall discharged HF183 overnight which transported to the surf zone. These findings support that there may be lowest achievable limits of surf zone HF183 owing to several chronic and permanent, perhaps diurnal, low concentration sources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.673190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269448PMC
June 2021

Sources of Low Level Human Fecal Markers in Recreational Waters of Two Santa Barbara, CA Beaches: Roles of WWTP Outfalls and Swimmers.

Water Res 2021 Jun 20;202:117378. Epub 2021 Jun 20.

Bren School of Environmental Science & Management, University of California, Santa Barbara, United states. Electronic address:

Worldwide, fecal indicator bacteria (FIB) evidence coastal water contamination for which sources are unknown. Here, for two FIB-impacted Santa Barbara recreational beaches, hypothesized fecal sources were investigated over three dry seasons (summers) using nearly 2000 field samples of water (ocean, creek, groundwater), sand, sediments, effluent and fecal sources. In years 1 and 2, gull and dog feces were identified as the probable main FIB sources to surf zone waters, yet HF183 human fecal markers were consistently detected. Determining HF183 sources was therefore prioritized, via year 3 sub-studies. In lower watersheds, human and dog wastes were mobilized by small storms into creeks, but no storm drain outfalls or creeks discharged into surf zones. Beach area bathrooms, sewers, and a septic system were not sources: dye tracing discounted hydraulic connections, and shallow groundwater was uncontaminated. Sediments from coastal creeks and downstream scour ponds, nearshore marine sediments, and sands from inter- and supratidal zones contained neither HF183 nor pathogens. Two nearby wastewater treatment plant (WWTP) outfalls discharged HF183 into plumes that were either deep or distant with uncertain onshore transport. Regardless, local sources were evidenced, as surf zone HF183 detection rates mostly exceeded those offshore and nearshore (around boat anchorages). The presence of swimmers was associated with surf zone HF183, as swimmer counts (on weekdays, holidays, weekends, and during races) significantly correlated (p<0.05, n = 196) to HF183 detections. Besides comprehensively assessing all possible fecal sources, this study provides new explanations of chronic low-level human markers in recreational beach surf zones, suggesting likely lowest achievable HF183 thresholds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2021.117378DOI Listing
June 2021

The prognostic value of myocardial injury in COVID-19 patients and associated characteristics.

Immun Inflamm Dis 2021 Jul 9. Epub 2021 Jul 9.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Since December 2019, coronavirus disease 2019 (COVID-19) has emerged as an international pandemic. COVID-19 patients with myocardial injury might need special attention. However, an understanding on this aspect remains unclear. This study aimed to illustrate clinical characteristics and the prognostic value of myocardial injury to COVID-19 patients.

Methods: This retrospective, single-center study finally included 304 hospitalized COVID-19 cases confirmed by real-time reverse-transcriptase polymerase chain reaction from January 11 to March 25, 2020. Myocardial injury was determined by serum high-sensitivity troponin I (Hs-TnI). The primary endpoint was COVID-19-associated mortality.

Results: Of 304 COVID-19 patients (median age, 65 years; 52.6% males), 88 patients (27.3%) died (61 patients with myocardial injury, 27 patients without myocardial injury on admission). COVID-19 patients with myocardial injury had more comorbidities (hypertension, chronic obstructive pulmonary disease, cardiovascular disease, and cerebrovascular disease); lower lymphocyte counts, higher C-reactive protein (CRP; median, 84.9 vs. 28.5 mg/L; p < .001), procalcitonin levels (median, 0.29 vs. 0.06 ng/ml; p < .001), inflammatory and immune response markers; more frequent need for noninvasive ventilation, invasive mechanical ventilation; and was associated with higher mortality incidence (hazard ratio [HR] = 7.02; 95% confidence interval [CI], 4.45-11.08; p < .001) than those without myocardial injury. Myocardial injury (HR = 4.55; 95% CI, 2.49-8.31; p < .001), senior age, CRP levels, and novel coronavirus pneumonia types on admission were independent predictors to mortality in COVID-19 patients.

Conclusions: COVID-19 patients with myocardial injury on admission is associated with more severe clinical presentation and biomarkers. Myocardial injury and higher Hs-TnI are both strongest independent predictors to COVID-19-related mortality after adjusting confounding factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/iid3.484DOI Listing
July 2021

Deciphering variation of 239 elite japonica rice genomes for whole genome sequences-enabled breeding.

Genomics 2021 Jul 6;113(5):3083-3091. Epub 2021 Jul 6.

Huazhi Biotech Co. Ltd., Changsha, Hunan 410125, China; China Southern Regional Collaborative Innovation Center for Cereal and Oil Crops, College of Agriculture, Hunan Agricultural University, Changsha, Hunan 410125, China. Electronic address:

Revealing genomic variation of representative and diverse germplasm is the cornerstone of deploying genomics information into genetic improvement programs of species of agricultural importance. Here we report the re-sequencing of 239 japonica rice elites representing the genetic diversity of japonica germplasm in China, Japan and Korea. A total of 4.8 million SNPs and PAV of 35,634 genes were identified. The elites from Japan and Korea are closely related and relatively less diverse than those from China. A japonica rice pan-genome was constructed, and 35 Mb non-redundant novel sequences were identified, from which 1131 novel genes were predicted. Strong selection signals of genomic regions were detected on most of the chromosomes. The heading date genes Hd1 and Hd3a have been artificially selected during the breeding process. The results from this study lay the foundation for future whole genome sequences-enabled breeding in rice and provide a paradigm for other species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2021.07.002DOI Listing
July 2021

Optimal Pre-Filtering for Improving Facebook Shared Images.

IEEE Trans Image Process 2021 12;30:6292-6306. Epub 2021 Jul 12.

Online Social Networks (OSNs) have attracted a huge number of users, who store and share various images on a daily basis. As a well-known fact, most OSN platforms apply a series of lossy operations on the uploaded images, which could severely degrade the quality of the shared images, negatively affecting the user experiences. In this work, we consider the problem of significantly improving OSN-shared images through applying an optimal pre-filtering prior to image sharing, without any cooperation from the OSN platform itself. Facebook, as one of the most popular and representative OSNs, is chosen as the platform to present our designed pre-filtering strategy. We first treat Facebook as a black box, and thoroughly recover its mechanism of processing color images. Based on the precise knowledge on the image processing pipeline on Facebook, we design the pre-filter under an optimization framework, minimizing the end-to-end distortion between the shared image and the original one. Compared with the directly shared images, our proposed pre-filtering-then-sharing strategy brings significant improvements in terms of both quantitative and qualitative metrics. Extensive experimental results are provided to show the superiority of our proposed method. Finally, we discuss the strategy on how to extend our proposed technique to other OSN platforms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TIP.2021.3093794DOI Listing
July 2021

Additive printing of recyclable anti-counterfeiting patterns with sol-gel cellulose nanocrystal inks.

Nanoscale 2021 Jul;13(27):11808-11816

College of Chemistry and Molecular Sciences, Engineering Research Center of Natural Polymer-based Medical Materials in Hubei Province, and Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, 430072, China.

The assembly of cellulose nanocrystals (CNCs) that produce attractive structural color shows great potential in anti-counterfeiting application, but their processability and recyclability remain unsatisfactory due to the strong hydrogen bonds between CNCs. For the first time, optical anti-counterfeiting patterns are obtained by additive printing of surface-functionalized CNC inks (CNC-DC5700-NPES). The surface-functionalized CNC inks are prepared by sequential modification of CNCs with organosilane (DC5700) and polyoxyethylene ether (NPES), which show good flowability under shearing force and transform into a gel-like phase rapidly after printing, making possible ink-jet printing without additives. The printed patterns are transparent under natural light but show vivid interference color, showing anti-counterfeiting features between crossed polarizers. The texture and optical properties of the printed patterns can be facilely controlled by tuning the printing parameters, such as nozzle diameter, writing angle, and filling width. Moreover, the CNC-DC5700-NPES patterns with a core-shell structure could be collected in various solvents and reprinted after removing solvents. This work provided a new pathway for the preparation of optical anti-counterfeiting patterns from biomass resources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1nr01777bDOI Listing
July 2021

Antioxidant activity of novel casein-derived peptides with microbial proteases as characterized via Keap1-Nrf2 pathway in HepG2 cells.

J Microbiol Biotechnol 2021 Jun 28;31. Epub 2021 Jun 28.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.

Casein-derived antioxidant peptides by using microbial proteases have gained increasing attention. Combination of two microbial proteases, Protin SD-NY10 and Protease A "Amano" 2SD, was employed to hydrolyze casein to obtain potential antioxidant peptides that were identified by LC-MS/MS, chemically synthesized and characterized in a oxidatively damaged HepG2 cell model. Four peptides, YQLD, FSDIPNPIGSEN, FSDIPNPIGSE, YFYP were found to possess high 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability. Evaluation with HepG2 cells showed that the 4 peptides at low concentrations (< 1.0 mg/mL) protected the cells against oxidative damage. The 4 peptides exhibited different levels of antioxidant activity by stimulating mRNA and protein expression of the antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), as well as nuclear factor erythroid-2-related factor 2 (Nrf2), but decreasing the mRNA expression of Kelch-like ECH-associated protein 1 (Keap1). Furthermore, these peptides decreased production of reactive oxygen species (ROS) and malondialdehyde (MDA), but increased glutathione (GSH) production in HepG2 cells. Therefore, the 4 casein-derived peptides obtained by using microbial proteases exhibited different antioxidant activity by activating the Keap1-Nrf2 signaling pathway, and they could serve as potential antioxidant agents in functional foods or pharmaceutic preparation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4014/jmb.2104.04013DOI Listing
June 2021

Depression-like behavior associated with E/I imbalance of mPFC and amygdala without TRPC channels in mice of knockout IL-10 from microglia.

Brain Behav Immun 2021 Jul 2. Epub 2021 Jul 2.

Medical School, Nankai University, Tianjin, China; Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Tianjin, China. Electronic address:

Depression has a growing impact on public health. Accumulating evidence supports an association between depression and increased immune system activity. IL-10 is a key cytokine that inhibits excessive inflammatory responses and is related to the anti-inflammatory and protective functions of the central nervous system (CNS). Cx3cr1IL-10 mice were used in our study. We aimed to identify the role of IL-10 in microglia in depression and anxiety-like behavior. We performed a series of behavioral tests on the mice; the Cx3cr1IL-10 male mice showed depression- and anxiety-like behavior compared with the littermates. The expression of transient receptor potential canonical 5 (TRPC5) decreased in both the medial prefrontal cortex (mPFC) and amygdala regions. The cytokines IL-1β and IL-6 increased, and IL-10 was decreased by western blotting. The knockout mice showed different trends in the effects of synaptic proteins. In the mPFC, IL-10 knockout induced a decrease in NR2B and synaptophysin; in the amygdala region, there was a significant increase in NR2B and PSD95. IL-10 knockout from microglia induced a decrease in GAD67 and parvalbumin (Pv) in the mPFC, but not in the amygdala. Our results showed enhanced depression and anxiety-like behavior in the Cx3cr1 IL-10 mice, which could be related to an imbalance in local excitatory and inhibitory transmission, as well as neuroinflammation in the mPFC and amygdala. This imbalance was associated with increased local inflammation. Although many studies have demonstrated the role of TRPC channels in emotional responses, our study showed that TRPC was not involved in this process in Cx3cr1IL-10 mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbi.2021.06.015DOI Listing
July 2021

Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis.

Front Microbiol 2021 18;12:663019. Epub 2021 Jun 18.

Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, China.

The outbreaks of enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) have emerged as an emergency of global health due to its association with fatal encephalitis and subsequent neurogenic pulmonary edema; however, the molecular characteristics and pathological features underlying EV71-associated encephalitis and pulmonary edema remain largely unknown. In this study, we performed a proteomic analysis of fresh brain and lung tissues from EV71-infected mice at 7 days post infection. We detected a perturbed expression of 148 proteins in the brain and 78 proteins in the lung after EV71 expression. Further analysis showed that the dysregulated proteins in the brain are involved in a variety of fundamental biological pathways, including complement and coagulation cascades, innate and adaptive immune responses, platelet activation, and nitrogen metabolism, and those proteins in the lung participate in innate and adaptive immune responses, phagosome, arginine biosynthesis, and hypoxia-inducible factor 1 signaling pathway. Our results suggested that immune activation, complement and coagulation dysfunction, platelet activation, imbalance of nitrogen metabolism, and hypoxia could be involved in the pathogenesis of EV71, which explains the major clinical manifestation of hyperinflammatory status of severe HFMD cases. Our study provides further understanding of the molecular basis of EV71 pathogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.663019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249819PMC
June 2021
-->