Publications by authors named "Dong Jiang"

415 Publications

An All-Inside Repair Technique for Unstable Type-IV Lateral Meniscal Posterior Root Tear.

Arthrosc Tech 2022 Jul 21;11(7):e1317-e1320. Epub 2022 Jun 21.

Sports Medicine Department, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, Beijing, China.

Lateral meniscal posterior root tears are defined as tears of meniscus within 9 mm from the bony root attachment. Unrepaired root tears significantly compromise the biomechanical functions of the meniscus, resulting in early and accelerated osteoarthritis. Several authors have described various techniques to reattach the posterior root of the lateral meniscus, and decent short-term results have been observed. Although most previous techniques are capable of repairing radial root tears, difficulties are encountered when repairing oblique type IV tears. In this technique note, the author describes an efficient side-to-side repair technique using the FAST-FIX system, which is practical in repairing the type IV tears of the lateral meniscus. This technique includes certain sequence of the stitches and skills of controlling the root remnant, which makes it easy to penetrate the meniscus and to achieve anatomic repair.
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http://dx.doi.org/10.1016/j.eats.2022.03.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353532PMC
July 2022

A Rare Case of Acute Infectious Purpura Fulminans Caused by and Human Herpesvirus Type 5.

J Inflamm Res 2022 26;15:4251-4260. Epub 2022 Jul 26.

Laboratory Bacteria Room, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, People's Republic of China.

Background: Purpura fulminans (PF), a rare, life-threatening disorder, is a hematological emergency in which there is skin necrosis, disseminated intravascular coagulation (DIC), and protein C deficiency. In PF, the skin necrosis and DIC are secondary to protein C deficiency. This may progress rapidly to multiorgan failure caused by the thrombotic occlusion of small- and medium-sized blood vessels.

Case Report: This article presents the case of a 22-year-old male with fever as well as necrotic and purpuric skin lesions. The ultrasound and computed tomography scans revealed infections in the skin wounds as well as venous microthrombosis and thrombosis in multiple intracranial and pulmonary vessels. The laboratory tests showed signs of sepsis, thrombocytopenia, an abnormal decrease in protein C and antithrombin III, DIC, multiple organ and system failures, gastric varices, and gastrointestinal hemorrhage. The blood, sputum, and secretions under the skin lesions were cultured and were positive for . The results of the high-throughput genetic testing of the pathogenic microorganism DNA were consistent. In addition, human herpesvirus type 5 was detected. The histopathological examination of the skin lesions revealed pathological features consistent with PF. After successful treatment by the departments of Dermatology, Emergency Critical Care Medicine, and the Intensive Care Unit, the patient was discharged after 67 days of hospitalization.

Conclusion: Adults with acquired protein C and/or S deficiency states, including certain bacterial and viral infections, who drink alcohol and take varieties of non-steroidal anti-inflammatory analgesics at the same time, may develop acute infectious PF. Clinicians should be aware of this for early diagnosis and treatment.
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http://dx.doi.org/10.2147/JIR.S369986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340174PMC
July 2022

Vision-Based Detection of Bolt Loosening Using YOLOv5.

Sensors (Basel) 2022 Jul 11;22(14). Epub 2022 Jul 11.

School of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China.

Bolted connections have been widely applied in engineering structures, loosening will happen when bolted connections are subjected to continuous cyclic load, and a significant rotation between the nut and the bolt can be observed. Combining deep learning with machine vision, a bolt loosening detection method based on the fifth version of You Only Look Once (YOLOv5) is proposed, and the rotation of the nut is identified to detect the bolt loosening. Two different circular markers are added to the bolt and the nut separately, and then YOLOv5 is used to identify the circular markers, and the rotation angle of the nut against the bolt is calculated according to the center coordinate of each predicted box. A bolted connection structure is adopted to illustrate the effectiveness of the method. First, 200 images containing bolts and circular markers are collected to make the dataset, which is divided into a training set, verification set and test set. Second, YOLOv5 is used to train the model; the precision rate and recall rate are respectively 99.8% and 100%. Finally, the robustness of the proposed method in different shooting environments is verified by changing the shooting distance, shooting angle and light condition. When using this method to detect the bolt loosening angle, the minimum identifiable angle is 1°, and the maximum detection error is 5.91% when the camera is tilted 45°. The experimental results show that the proposed method can detect the loosening angle of the bolted connection with high accuracy; especially, the tiny angle of bolt loosening can be identified. Even under some difficult shooting conditions, the method still works. The early stage of bolt loosening can be detected by measuring the rotation angle of the nut against the bolt.
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http://dx.doi.org/10.3390/s22145184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319482PMC
July 2022

Optimizing Nitrogen and Seed Rate Combination for Improving Grain Yield and Nitrogen Uptake Efficiency in Winter Wheat.

Plants (Basel) 2022 Jun 30;11(13). Epub 2022 Jun 30.

National Technique Innovation Centre for Regional Wheat Production, Key Laboratory of Crop and Ecophysiology in Southern China, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China.

Nitrogen (N) supply and seed rate (SR) are two essential factors that affect the accumulation and partitioning of N and dry matter (DM) and, therefore, grain yield (GY) and N use efficiency (NUE). The objective of this experiment was to optimize N application and SR to regulate wheat growth and increase both GY and NU. The results revealed that net photosynthetic rate (Pn), stomatal conductance (Gs), chlorophyll content, and activities of metabolic enzymes (NR and GS) significantly increased with increasing of N levels while decreasing SR. Plant tillers, GY, DM before anthesis, and N translocation, N agronomic efficiency (NA), N recovery efficiency (NR), and N uptake efficiency (NUP) were highest in a combined treatment of N and SR. However, N levels beyond 235 kg ha significantly decreased NA, NR, and NUP. By increasing SR from 135 to 180 kg ha an increase of 12.9 % and 9.1% GY and NUPE, respectively, was observed. Based on this result, we estimate that 1 kg N ha might be replaced by an increase of approximately 0.6 kg ha SR. Our study suggested that using a combination of N and SR (N + SR) could attain maximum GY and improve NU parameters.
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http://dx.doi.org/10.3390/plants11131745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269081PMC
June 2022

P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.

Cancers (Basel) 2022 Jul 1;14(13). Epub 2022 Jul 1.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215000, China.

Lung cancer is associated with the greatest number of cancer-related deaths worldwide. Lung adenocarcinoma (LUAD) accounts for 85% of all cases of lung cancer. Despite recent advances in treatment, the 5-year survival rate remains less than 15%. Thus, the diagnostic and therapeutic role of LUAD remain to be further studied. The prolyl 3-hydroxylase family member 4 (P3H4) is involved in various cancers, but little is known about its role in LUAD. Our study demonstrated that the P3H4 gene was upregulated in LUAD. Clinically, the expression of P3H4 was positively correlated with an advanced TNM stage and shorter survival. Functionally, P3H4 plays a significant role in the metastasis and proliferation of LUAD both in vitro and in vivo. Mechanistically, P3H4 might interact with EGFR to regulate the metabolic substances. Our study indicated that P3H4 is a critical gene in the malignant progression of LUAD and represents a potential biomarker and therapeutic target.
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http://dx.doi.org/10.3390/cancers14133243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264976PMC
July 2022

Comparison of the diagnostic value of measurements of transverse syndesmotic interval and 'lambda sign' in distinguishing latent syndesmotic diastasis in chronic lateral ankle instability: a cross-sectional study of 188 cases.

Arch Orthop Trauma Surg 2022 Jun 21. Epub 2022 Jun 21.

Radiology Department, Peking University Third Hospital, No.49 North Garden Road, Haidian, Beijing, 100191, China.

Introduction: Chronic lateral ankle instability (CLAI) could accompany with latent syndesmotic diastasis (LSD), which is difficult to distinguish before surgery. Tibiofibular interval width and extravasation of joint fluid ('lambda sign') on MRI are widely used in the diagnosis of syndesmotic injury, but the reliability of these methods in distinguishing the associated LSD in CLAI was rarely studied. Our objective was to compare the diagnostic value of the measurement of the transverse tibiofibular interval and 'lambda sign' on MRI in distinguishing LSD in CLAI and to investigate the radiological predictor that best matched the intraoperatively measured syndesmotic width.

Methods: 138 CLAI patients undergoing arthroscopy in our institute from March 2017 to June 2020 were enrolled (CLAI group). Anterior space width (ASW) and posterior space width (PSW) at 10 mm layer above tibial articular and fluid height above tibial articular surface (FH) were measured on preoperative MRI. The same parameters were measured on MRI of 50 healthy volunteers as control group. At arthroscopy, syndesmotic width was measured and the patients were divided into arthroscopic widening (AW) and arthroscopic normal (AN) subgroup taking 2 mm as critical value. The CLAI group was compared with the control group to explore the interval changes related to CLAI. The AW and AN subgroups were compared to explore the potential diagnostic indicators and reference values for the LSD.

Results: All parameters showed significant difference between CLAI group and control group (p < 0.05), but only PSW (p = 0.004) showed significant difference between AW and AN subgroups other than FH (p = 0.461). Only PSW was involved in formula of multiple-factor analysis (p = 0.005; OR, 1.819; 95%CI, 1.196-2.767). ROC analysis showed critical value of PSW was 3.8 mm (sensitivity, 66%; specificity, 66%; accuracy, 66.7%), while accuracy of lambda sign was 41.3%.

Conclusions: Transverse tibiofibular interval measurements were more reliable than the 'lambda sign' in distinguishing associated LSD in CLAI patients. The PSW ≥ 3.8 mm could be a predictor of syndesmotic diastasis.
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http://dx.doi.org/10.1007/s00402-022-04500-7DOI Listing
June 2022

Simple and rapid separation of haematococcus pluvialis and ciliate based on the dean-coupled inertial microfluidics.

J Sep Sci 2022 Jun 16. Epub 2022 Jun 16.

Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing, 100083, China.

Astaxanthin with high antioxidant activity is of great practical value and Haematococcus pluvialis is recognized as the best natural astaxanthin producers. The yield of Haematococcus pluvialis were often affected by the ciliate during its production, however the use of biochemical pesticides might have great impact on Haematococcus pluvialis. Therefore, a simple microfluidic chip with the spiral microchannel was developed for continuous-flow physical separation of ∼10 μm ciliate from ∼30 μm Haematococcus pluvialis since their different sizes resulted in different equilibrium positions in the channel due to the Dean-coupled inertial migration. First, a spiral microchannel with a width of 700 μm and a height of 130 μm in the microfluidic chip was developed using 3D printing and verified to completely separate polystyrene particles of 10 μm from those of 30 μm. Then, this microfluidic chip was used to separate actual sample, and experimental results showed that ∼80% of ciliate was continuously separated from Haematococcus pluvialis at a flow rate of 2.8 mL/min. More importantly, no additional biochemical reagents were used and the activity of Haematococcus pluvialis was not affected. This microfluidic chip featured with simple design, automatic operation and small size is promising for purification and breeding of Haematococcus pluvialis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jssc.202200308DOI Listing
June 2022

Anatomic reconstruction using the autologous gracilis tendon achieved less sprain recurrence than the Broström-Gould procedure but delayed recovery in chronic lateral ankle instability.

Knee Surg Sports Traumatol Arthrosc 2022 Jun 8. Epub 2022 Jun 8.

Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, No.49 North Garden Road, Beijing, 100191, China.

Purpose: To compare the return-to-activity and long-term clinical outcomes between anatomic lateral ligament reconstruction using the autologous gracilis tendon and modified Broström-Gould (MBG) procedure in chronic lateral ankle instability (CLAI). It was hypothesised that there was no difference between the two techniques.

Methods: From 2013 to 2018, 30 CLAI patients with grade III joint instability confirmed by anterior drawer test underwent anatomic reconstruction of lateral ankle ligament with the autologous gracilis tendon (reconstruction group) in our institute. Another 30 patients undergoing MBG procedure (MBG group) were matched in a 1:1 ratio based on demographic parameters. The post-operative American Orthopaedic Foot and Ankle Society (AOFAS) score, visual analogue scale (VAS) pain score, Tegner activity score, Karlsson-Peterson score, surgical complications, return-to-activities and work were retrospectively evaluated and compared between the two groups.

Results: All subjective scores significantly improved after the operation (all with p < 0.001) without difference between the two groups (all n.s.). The MBG group showed a significantly higher proportion of postoperative sprain recurrence than the reconstruction group (26.7% vs. 0, p = 0.002). The reconstruction group showed a significantly longer period to start walking with full weight-bearing (10.5 ± 6.9 vs. 7.0 ± 3.1 weeks, p = 0.015), jogging (17.1 ± 8.9 vs. 12.7 ± 6.9 weeks, p = 0.043) and return-to-work (13.5 ± 12.6 vs. 8.0 ± 4.7 weeks, p = 0.039) than the MBG group.

Conclusions: Both anatomic reconstruction using the autologous gracilis tendon and MBG procedure could equally achieved reliable long-term clinical outcomes and the tendon reconstruction showed a relatively lower incidence of postoperative sprain recurrence but delayed recovery to walking, jogging and return-to-work. The MBG procedure was still the first choice with rapid recovery but the tendon reconstruction was recommended for patients with higher strength demand.

Level Of Evidence: III.
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http://dx.doi.org/10.1007/s00167-022-07011-5DOI Listing
June 2022

Factors Influencing Trace Element Levels in the Blood of Tin Smelting Workers.

J Occup Environ Med 2022 Jul 8;64(7):e403-e408. Epub 2022 Jun 8.

From the Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Control and Prevention (Drs L. Zhang, Wang, H. Zhang, X. Liu, Zhong, D. Liu, Jiang, Y. Zhu, B. Zhu, and Han); and Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University (Drs L. Zhang, Wang, and B. Zhu), Nanjing; Kunshan Municipal Center for Disease Prevention and Control (Dr Shen); Center for Global Health, Nanjing Medical University (Dr B. Zhu); and Jiangsu Province Engineering Research Center of Public Health Emergency (Dr B. Zhu), China.

Background: This study is to assess the correlation between blood concentration ranges of eight elements of tin smelting workers from Guangxi Liuzhou and their job type, working years, age, and sex.

Methods: We collected blood samples of 218 tin smelting workers from a Chinese tin smelting factory and determined the levels of elements by inductively coupled plasma mass spectrometry.

Results: Within the blood concentrations of eight metal elements of the objects, the blood concentration of copper and zinc is affected by the job type of comprehensive work; that of arsenic and mercury is affected by refining; and that of chromium, cadmium, and lead is affected by primary smelting.

Conclusions: We present the remarkable influence of four job types on the blood concentration of seven trace elements.
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http://dx.doi.org/10.1097/JOM.0000000000002554DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301975PMC
July 2022

Evaluation of an intelligent artificial climate chamber for high-throughput crop phenotyping in wheat.

Plant Methods 2022 Jun 7;18(1):77. Epub 2022 Jun 7.

College of Engineering, Nanjing Agricultural University, Nanjing, 210031, China.

Background: The superposition of COVID-19 and climate change has brought great challenges to global food security. As a major economic crop in the world, studying its phenotype to cultivate high-quality wheat varieties is an important way to increase grain yield. However, most of the existing phenotyping platforms have the disadvantages of high construction and maintenance costs, immobile and limited in use by climatic factors, while the traditional climate chambers lack phenotypic data acquisition, which makes crop phenotyping research and development difficult. Crop breeding progress is slow. At present, there is an urgent need to develop a low-cost, easy-to-promote, climate- and site-independent facility that combines the functions of crop cultivation and phenotype acquisition. We propose a movable cabin-type intelligent artificial climate chamber, and build an environmental control system, a crop phenotype monitoring system, and a crop phenotype acquisition system.

Result: We selected two wheat varieties with different early vigor to carry out the cultivation experiments and phenotype acquisition of wheat under different nitrogen fertilizer application rates in an intelligent artificial climate chamber. With the help of the crop phenotype acquisition system, images of wheat at the trefoil stage, pre-tillering stage, late tillering stage and jointing stage were collected, and then the phenotypic information including wheat leaf area, plant height, and canopy temperature were extracted by the crop type acquisition system. We compared systematic and manual measurements of crop phenotypes for wheat phenotypes. The results of the analysis showed that the systematic measurements of leaf area, plant height and canopy temperature of wheat in four growth periods were highly correlated with the artificial measurements. The correlation coefficient (r) is positive, and the determination coefficient (R) is greater than 0.7156. The root mean square error (RSME) is less than 2.42. Among them, the crop phenotype-based collection system has the smallest measurement error for the phenotypic characteristics of wheat trefoil stage. The canopy temperature RSME is only 0.261. The systematic measurement values of wheat phenotypic characteristics were significantly positively correlated with the artificial measurement values, the fitting degree was good, and the errors were all within the acceptable range. The experiment showed that the phenotypic data obtained with the intelligent artificial climate chamber has high accuracy. We verified the feasibility of wheat cultivation and phenotype acquisition based on intelligent artificial climate chamber.

Conclusion: It is feasible to study wheat cultivation and canopy phenotype with the help of intelligent artificial climate chamber. Based on a variety of environmental monitoring sensors and environmental regulation equipment, the growth environment factors of crops can be adjusted. Based on high-precision mechanical transmission and multi-dimensional imaging sensors, crop images can be collected to extract crop phenotype information. Its use is not limited by environmental and climatic factors. Therefore, the intelligent artificial climate chamber is expected to be a powerful tool for breeders to develop excellent germplasm varieties.
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http://dx.doi.org/10.1186/s13007-022-00916-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170875PMC
June 2022

Proximal and remote sensing in plant phenomics: 20 years of progress, challenges, and perspectives.

Plant Commun 2022 Jun 2:100344. Epub 2022 Jun 2.

Plant Phenomics Research Centre, Academy for Advanced Interdisciplinary Studies, National Engineering and Technology Center for Information Agriculture, Collaborative Innovation Centre for Modern Crop Production co-sponsored by Province and Ministry, Nanjing Agricultural University, Address: No. 1 Weigang, Xuanwu District, Nanjing 210095, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China; Sanya Research Institute of Nanjing Agriculture University, Sanya 572024, China; Jiangsu Provincial Key Laboratory of Geographic Information Science and Technology, International Institute for Earth System Sciences, Nanjing University, Nanjing, Jiangsu 210023, China. Electronic address:

Plant phenomics (PP) has been recognized as a bottleneck in studying the interactions of genomics and environment on plants, limiting the progress of smart breeding and precise cultivation. High-throughput plant phenotyping is challenging owing to the spatio-temporal dynamics of traits. Proximal and remote sensing (PRS) techniques are increasingly used for plant phenotyping because of their advantages in multi-dimensional data acquisition and analysis. Substantial progress of PRS applications in PP has been observed over the last two decades and is analyzed here from an interdisciplinary perspective based on 2972 publications. This progress covers most aspects of PRS application in PP, including patterns of global spatial distribution and temporal dynamics, specific PRS technologies, phenotypic research fields, working environments, species, and traits. Subsequently, we demonstrate how to link PRS to multi-omics studies, including how to achieve multi-dimensional PRS data acquisition and processing, how to systematically integrate all kinds of phenotypic information and derive phenotypic knowledge with biological significance, and how to link PP to multi-omics association analysis. Finally, we identify three future perspectives for PRS-based PP: (1) strengthening the spatial and temporal consistency of PRS data, (2) exploring novel phenotypic traits, and (3) facilitating multi-omics communication.
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http://dx.doi.org/10.1016/j.xplc.2022.100344DOI Listing
June 2022

Value of 5-Hydroxymethylcytosine in HBV-Carrying High-Risk Hepatocellular Carcinoma Population: An Evaluation Based on Differential Analysis.

Comput Math Methods Med 2022 23;2022:4835417. Epub 2022 May 23.

Department of Disease Prevention and Control, The Third Affiliated Hospital of Naval Military Medical University, Shanghai 200000, China.

Objective: To clarify the application value of 5-hydroxymethylcytosine (5hmC) in evaluating the progression of chronic hepatitis B (CHB) to hepatocellular carcinoma (HCC) based on difference analysis.

Methods: A total of 180 patients were enrolled. Among them, 84 patients with chronic hepatitis B virus (HBV) infection while no progression to hepatocellular carcinoma (HCC) were included in the control group (CG), and 96 patients with HCC developed from HBV infection were included in the research group (RG). Two-thirds of the samples were used in the training set and 1/3 samples in the validation set to detect the level of 5hmC in both groups based on the modified nano-hmC-Seal technique. The expression levels of 5hmC-related genes TET2 and TET3 were quantified by qPCR, and the correlation between TET3 and 5hmC was analyzed by Pearson's correlation coefficients. Receiver operating characteristic (ROC) curves were drawn to evaluate the application value of the TET3-based 5hmC prediction model in the early diagnosis of HCC.

Results: (i) The expression of 5hmC in RG was lower than that in CG, no matter in the training set or the validation set. (ii) 5hmC was significantly enriched in the region between the transcription initiation site and the transcription end site but was depleted in the flanking region. (iii) 5hmC-related genes TET2 and TET3 were significantly downregulated in HCC patients, whether in the training set or the validation set. (iv) In both the training and validation sets, TET3 showed a positive association with 5hmC. (v) ROC analysis results showed that the 5hmC prediction model could be used to predict the progression of CHB to HCC (training set: AUC = 0.81, 0.729-0.893; validation set: AUC = 0.84, 0.739-0.936).

Conclusions: TET3 expression based on 5hmC sequencing is a landmark molecule for evaluating the progression of HCC in CHB patients, which is worthy of further study and promotion.
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http://dx.doi.org/10.1155/2022/4835417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150989PMC
June 2022

Cancer-Secreted Exosomal MiR-620 Inhibits ESCC Aerobic Glycolysis FOXM1/HER2 Pathway and Promotes Metastasis.

Front Oncol 2022 16;12:756109. Epub 2022 May 16.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Esophageal squamous cell carcinoma (ESCC) is a leading cause of cancer death worldwide. MicroRNAs (MiRNAs) have been reported to regulate cell functions through exosomes. Through the Gene Expression Omnibus (GEO) database, miR-620 was selected as a serum miRNA highly expressed in ESCC, but its detailed role in ESCC has not been explored. Tumor-secreted miRNAs have been reported to promote cancer metastasis through reprogramming the aerobic glycolysis of lung fibroblasts. Therefore, we intended to verify whether exosomal miR-620 secreted in ESCC cells may regulate the aerobic glycolysis of lung fibroblasts.

Methods: The effect of miR-620 on the aerobic glycolysis of ESCC cells was firstly verified through bioinformatics prediction and mechanism assays. Exosomes secreted from ESCC cells was detected, and the influence of exosomal miR-620 in regulating the aerobic glycolysis of lung fibroblasts was then verified both and .

Results: MiR-620 inhibited ESCC malignancy and suppressed the aerobic glycolysis of ESCC cells targeting Forkhead box M1 (FOXM1) and human epidermal growth factor receptor 2 (HER2). Moreover, exosomal miR-620 was highly secreted in ESCC and could regulate HFL1 aerobic glycolysis FOXM1/HER2 signaling. Furthermore, exosomal miR-620 could promote ESCC metastasis by reprogramming the aerobic glycolysis of lung fibroblasts (HFL1).

Conclusion: Exosomal miR-620 secreted by ESCC cells inhibited the aerobic glycolysis FOXM1/HER2 axis and promoted cancer metastasis.
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http://dx.doi.org/10.3389/fonc.2022.756109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148961PMC
May 2022

Midterm Outcomes of Talocalcaneal Coalition Arthroscopic Resection in Adults.

Foot Ankle Int 2022 Aug 26;43(8):1062-1069. Epub 2022 May 26.

Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Beijing, China.

Background: Arthroscopic resection of the talocalcaneal coalition (TCC) has been reported to be associated with good short-term outcomes. However, the midterm outcomes of this approach remain uncertain.

Methods: We performed a retrospective study of patients who underwent arthroscopic resection for symptomatic TCC. The patients were divided into 2 groups (group P, patients with isolated posterior facet coalition; and group MP, patients with both middle and posterior facet coalition). The preoperative and postoperative visual analog scale (VAS) scores for pain and American Orthopaedic Foot & Ankle Society (AOFAS) scale scores were calculated. The postoperative AOFAS and VAS scores between the 2 groups were analyzed. Patient satisfaction was also assessed.

Results: Thirty-two patients were included in this study. The mean age at the time of surgery was 26.0±8.5 years, and the mean follow-up period was 56.9±18.0 months. Thirteen (41%) patients were in group P, whereas 19 (59%) patients were in group MP. Postoperative VAS and AOFAS scores improved more significantly than preoperative scores. At the final follow-up, excellent and good subjective outcomes were attained in 26 patients (81%), fair and poor outcomes in 6 patients (19%). There were no statistical differences in the postoperative AOFAS (91.0±7.0 vs 85.8±10.8, = .532) and VAS score (2.1±1.7 vs 4.0±2.6, = .537) between patients with the ratio of coalition/posterior facet more than or less than 50%. There were no statistical differences in postoperative VAS score (1.8±1.3 vs 2.6±2.2, = .236) and AOFAS score (92.5±5.6 vs 89.2±8.7, = .297) between group P (n=13) and group MP (n=19), either. Three patients (9.4%) had complications, including these notable findings: 1 patient complained of restricted dorsal flexion, 1 with computed tomography-proven coalition recurrence, and 1 with partial tibial nerve injury.

Conclusion: We found that TCC arthroscopic resection was generally associated with reasonable outcomes at midterm follow-up.

Level Of Evidence: Level IV, case series.
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http://dx.doi.org/10.1177/10711007221092756DOI Listing
August 2022

Simultaneous Treatment of Osteochondral Lesion Does Not Affect the Mid- to Long-Term Outcomes of Ligament Repair for Acute Ankle Sprain: A Retrospective Comparative Study with a 3-11-Year Follow-up.

Front Surg 2022 9;9:816669. Epub 2022 May 9.

Department of Sports Medicine, Peking University Third Hospital, Beijing, China.

Purpose: This study aims to evaluate the mid- to long-term outcome of concurrent arthroscopic treatment of osteochondral lesion (OCL) and open anatomical repair of lateral ankle ligaments for severe acute ankle sprain patients and compare them to the outcome of those without OCL.

Methods: A total of 166 patients with grade III acute lateral ankle ligament injuries underwent concurrent ankle arthroscopy and open anatomic ligament repair. Forty-three patients (group A) with OCL underwent arthroscopic treatment followed by open ligament repair. A total of 105 patients (group B) without OCL were followed up as the control. The evaluation parameters included sports recovery, postoperative visual analog scale (VAS) pain score, American Orthopaedic Foot and Ankle Society (AOFAS) score, Tegner score, sprain recurrence, satisfaction, and range of motion. Patients in group A were then subgroup-analyzed according to age, sex, body mass index, injury side, OCL location, and stage (Ferkel and Cheng's staging system).

Results: The postoperative exercise level of the two groups recovered to more than 90% of the normal level (91.2% ± 11.2% in group A and 90.9% ± 13.3% in group B, n.s.). The average time of group A and group B to return to preinjury sports activity was respectively 4.4 ± 1.0 months and 4.4 ± 1.2 months with no significant difference ( = 0.716). No significant differences were found in the preoperation VAS pain score, AOFAS score, and Tegner score between the two groups. The postoperative VAS pain score in group A was significantly higher than that in group B (0.8 ± 1.7 vs. 0.3 ± 0.8,  = 0.027), but the difference was not clinically important. The postoperative VAS pain score of patients with stage D-F lesions was significantly higher than that of patients with stage B-C lesions (1.3 ± 2.1 vs. 0.3 ± 0.9, = 0.038).

Conclusions: For the severe acute ankle sprain combined with OCL, the simultaneous arthroscopic treatment and open lateral ankle ligament repair achieved good mid- to long-term outcomes. Except that the pain was more pronounced than in the control group, there were no differences in other outcomes. Postoperative pain was positively correlated with the grade of OCL.
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http://dx.doi.org/10.3389/fsurg.2022.816669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124844PMC
May 2022

Metal-Organic Framework-Derived Graphene Mesh: a Robust Scaffold for Highly Exposed Fe-N Active Sites toward an Excellent Oxygen Reduction Catalyst in Acid Media.

J Am Chem Soc 2022 Jun 23;144(21):9280-9291. Epub 2022 May 23.

Kagami Memorial Institute for Materials Science and Engineering, Waseda University, Nishi-Waseda 2-8-26, Shinjuku-ku, Tokyo 169-0051, Japan.

This study demonstrates a special ultrathin N-doped graphene nanomesh (NGM) as a robust scaffold for highly exposed Fe-N active sites. Significantly, the pore sizes of the NGM can be elaborately regulated by adjusting the thermal exfoliation conditions to simultaneously disperse and anchor Fe-N moieties, ultimately leading to highly loaded Fe single-atom catalysts (SA-Fe-NGM) and a highly exposed morphology. The SA-Fe-NGM is found to deliver a superior oxygen reduction reaction (ORR) activity in acidic media (half-wave potential = 0.83 V RHE) and a high power density of 634 mW cm in the H/O fuel cell test. First-principles calculations further elucidate the possible catalytic mechanism for ORR based on the identified Fe-N active sites and the pore size distribution analysis. This work provides a novel strategy for constructing highly exposed transition metals and nitrogen co-doped carbon materials (M-N-C) catalysts for extended electrocatalytic and energy storage applications.
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http://dx.doi.org/10.1021/jacs.2c00719DOI Listing
June 2022

Modelling armed conflict risk under climate change with machine learning and time-series data.

Nat Commun 2022 05 20;13(1):2839. Epub 2022 May 20.

Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, 6150, Perth, WA, Australia.

Understanding the risk of armed conflict is essential for promoting peace. Although the relationship between climate variability and armed conflict has been studied by the research community for decades with quantitative and qualitative methods at different spatial and temporal scales, causal linkages at a global scale remain poorly understood. Here we adopt a quantitative modelling framework based on machine learning to infer potential causal linkages from high-frequency time-series data and simulate the risk of armed conflict worldwide from 2000-2015. Our results reveal that the risk of armed conflict is primarily influenced by stable background contexts with complex patterns, followed by climate deviations related covariates. The inferred patterns show that positive temperature deviations or precipitation extremes are associated with increased risk of armed conflict worldwide. Our findings indicate that a better understanding of climate-conflict linkages at the global scale enhances the spatiotemporal modelling capacity for the risk of armed conflict.
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http://dx.doi.org/10.1038/s41467-022-30356-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123163PMC
May 2022

Preoperative diagnosis and prediction of microvascular invasion in hepatocellularcarcinoma by ultrasound elastography.

BMC Med Imaging 2022 05 13;22(1):88. Epub 2022 May 13.

Department of Ultrasound, Eastern Hepatobiliary Surgery Hospital Affiliated to Naval Medical University, Shanghai, China.

Background: To assess the values of two elastography techniques combined with serological examination and clinical features in preoperative diagnosis of microvascular invasion in HCC patients.

Methods: A total of 74 patients with single Hepatocellular carcinoma (HCC) were included in this study. Shear wave measurement and real-time tissue elastography were used to evaluate the hardness of tumor-adjacent tissues and tumor tissues, as well as the strain rate ratio per lesion before surgery. According to the pathological results, the ultrasound parameters and clinical laboratory indicators related to microvascular invasion were analyzed, and the effectiveness of each parameter in predicting the occurrence of microvascular invasion was compared.

Results: 33/74 patients exhibited microvascular invasion. Univariate analysis showed that the hardness of tumor-adjacent tissues (P = 0.003), elastic strain rate ratio (P = 0.032), maximum tumor diameter (P < 0.001), and alpha-fetoprotein (AFP) level (P = 0.007) was significantly different in the patients with and without microvascular invasion. The binary logistic regression analysis showed that the maximum tumor diameter (P = 0.001) was an independent risk factor for predicting microvascular invasion, while the hardness of tumor-adjacent tissues (P = 0.028) was a protective factor. The receiver operating characteristic (ROC) curve showed that the area under the curve (AUC) of the hardness of tumor-adjacent tissues, the maximum diameter of the tumor, and the predictive model Logit(P) in predicting the occurrence of MVI was 0.718, 0.775 and 0.806, respectively.

Conclusion: The hardness of tumor-adjacent tissues, maximum tumor diameter, and the preoperative prediction model predict the occurrence of MVI in HCC patients.
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http://dx.doi.org/10.1186/s12880-022-00819-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107229PMC
May 2022

Reliability analysis of exonic-breakpoint fusions identified by DNA sequencing for predicting the efficacy of targeted therapy in non-small cell lung cancer.

BMC Med 2022 05 10;20(1):160. Epub 2022 May 10.

Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.17 Panjiayuan Nanli, Beijing, 100021, China.

Background: Diverse genomic breakpoints of fusions that localize to intronic, exonic, or intergenic regions have been identified by DNA next-generation sequencing (NGS), but the role of exonic breakpoints remains elusive. We investigated whether exonic-breakpoint fusions could predict matched targeted therapy efficacy in non-small cell lung cancer (NSCLC).

Methods: NSCLC samples were analyzed by DNA NGS, RNA NGS, immunohistochemistry (IHC), and fluorescence in situ hybridization.

Results: Using DNA NGS, kinase fusions were identified in 685 of 7148 (9.6%) NSCLCs, with 74 harboring exonic-breakpoint fusions, mostly anaplastic lymphoma kinase (ALK) fusions. RNA NGS and IHC revealed that 11 of 55 (20%) exonic-breakpoint fusions generated no aberrant transcript/protein, possibly due to open reading frame disruption or different gene transcriptional orientations. Four cases of genomic-positive but RNA/protein-negative fusions were treated with matched targeted therapy, but progressive disease developed within 2 months. Nevertheless, 44 of 55 (80%) exonic-breakpoint fusions produced chimeric transcripts/proteins, possibly owing to various alternative splicing patterns, including exon skipping, alternative splice site selection, and intron retention. Most of these genomic- and RNA/protein-positive fusion cases showed a clinical response to matched targeted therapy. Particularly, there were no differences in objective response rate (P = 0.714) or median progression-free survival (P = 0.500) between intronic-breakpoint (n = 56) and exonic-breakpoint ALK fusion subtypes (n = 11) among ALK RNA/protein-validated patients who received first-line crizotinib.

Conclusions: Exonic-breakpoint fusions may generate in-frame fusion transcripts/proteins or not, and thus are unreliable for predicting the efficacy of targeted therapy, which highlights the necessity of implementing RNA or protein assays for functional validation in exonic-breakpoint fusion cases.
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http://dx.doi.org/10.1186/s12916-022-02362-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087946PMC
May 2022

Ultrasonic Methods for Brain Imaging: Techniques and Implications.

IEEE Trans Biomed Eng 2022 May 6;PP. Epub 2022 May 6.

Brain imaging technology is widely used in the diagnosis of brain diseases. Computed tomography and magnetic resonance imaging are the most common imaging modalities used for clinical brain imaging, whereas ultrasound is rarely used because the skull substantially reduces the incident energy of ultrasonic waves to levels too low for imaging. However, remarkable developments of novel technologies in ultrasound brain imaging have been achieved recently, including Doppler-based imaging, contrast agent imaging, ultrasound elastography, and phase compensation imaging. Doppler-based imaging, including ultrafast Doppler imaging and functional ultrasound, is able to obtain reliable blood flow information and has the best penetration depth and highest temporal resolution. Contrast agent brain imaging, including ultrasound localization microscopy, has the best spatial resolution, and its temporal resolution can be maintained within a few milliseconds. Ultrasound elastography reflects the stiffness of brain tissues. Phase correction imaging, such as time reversal mirror and spatiotemporal inverse filter, aims at focusing smoothly in the skull. These methods have been widely performed on animal models, newborn children, and adults in preclinical studies, with results demonstrating great potential in the diagnosis and treatment of brain diseases. This review discusses the ultrasound methods developed in recent years for brain imaging and highlights the promising future they hold.
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http://dx.doi.org/10.1109/TBME.2022.3173035DOI Listing
May 2022

Retractosomes: small extracellular vesicles generated from broken-off retraction fibers.

Cell Res 2022 May 4. Epub 2022 May 4.

State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing, China.

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http://dx.doi.org/10.1038/s41422-022-00666-2DOI Listing
May 2022

The immediate meniscal allograft transplantation achieved better chondroprotection and less meniscus degeneration than the conventional delayed transplantation in the long-term.

Knee Surg Sports Traumatol Arthrosc 2022 Apr 28. Epub 2022 Apr 28.

Sports Medicine Department, Beijing Key Laboratory of Sports Injuries, Peking University Third Hospital, 49 North Garden Rd., Haidian District, Beijing, 100191, People's Republic of China.

Purpose: The purpose of this study was to compare the long-term clinical and radiological outcomes between the immediate and delayed meniscus allograft transplantation (MAT).

Methods: Nine menisci were transplanted immediately after total meniscectomy (immediate group, IM), and 10 menisci were delayed transplanted in patients with the median of 35 months (range 9-92 months) after total meniscectomy (delayed group, DE). Patient's subjective clinical outcomes including VAS, IKDC, Lysholm and Tegner scores as well as muscle strength measures were compared. Joint degeneration was evaluated by both radiographs to assess joint space width narrowing, Kellegren-Lawrence (KL) grade and MRI with T2 mapping sequences to quantitatively analyze both cartilage and meniscal allograft degeneration.

Results: The median follow-up time was 10.8 years (range 10-14 years). The IKDC (IM vs DE, 89.8 vs 80.9, n.s.) and Lysholm scores (IM vs DE, 87.7 vs 78.0, n.s.) were close in two groups, while the IM group showed slightly lower VAS (IM vs DE, 0.2 vs 1.5, p = 0.031), higher Tegner score (IM vs DE, 7 vs 3.5 p = 0.021) and better quadriceps muscle strength. The IM group had less joint space narrowing (IM vs DE, 0.35 mm vs 0.71 mm, n.s.), less KL grade progression (IM vs DE, 0.6 vs 1.7, p = 0.041) on radiographs and less chondral lesions development on MRIs (Cartilage Degeneration Index, IM vs DE, 252 vs 2038, p = 0.025). All meniscal grafts exhibited degeneration by showing grade 3 signal on MRI, and 4 (4/9) in the IM group and 8 (8/10) cases in the DE group. The T2 value of cartilage and meniscal allograft in the IM group was close to that of the healthy control and was significantly lower than that of the DE group.

Conclusion: Compared to the conventional delayed MAT, the immediate MAT achieved better cartilage and meniscus protection in the long-term, while its superiority in patient-reported outcomes was limited.

Level Of Evidence: IV.
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http://dx.doi.org/10.1007/s00167-022-06982-9DOI Listing
April 2022

Advances in the Application of Three-dimensional Printing for the Clinical Treatment of Osteoarticular Defects.

Curr Med Sci 2022 Jun 22;42(3):467-473. Epub 2022 Apr 22.

Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Beijing, 100191, China.

As a promising manufacturing technology, three-dimensional (3D) printing technology is widely used in the medical field. In the treatment of osteoarticular defects, the emergence of 3D printing technology provides a new option for the reconstruction of functional articular surfaces. At present, 3D printing technology has been used in clinical applications such as models, patient-specific instruments (PSIs), and customized implants to treat joint defects caused by trauma, sports injury, and tumors. This review summarizes the application status of 3D printing technology in the treatment of osteoarticular defects and discusses its advantages, disadvantages, and possible future research strategies.
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http://dx.doi.org/10.1007/s11596-022-2565-9DOI Listing
June 2022

Quantifying risk factors and potential geographic extent of African swine fever across the world.

PLoS One 2022 21;17(4):e0267128. Epub 2022 Apr 21.

School of Veterinary Medicine, Centre for Biosecurity and One Health, Murdoch University, Perth, Australia.

African swine fever (ASF) has spread to many countries in Africa, Europe and Asia in the past decades. However, the potential geographic extent of ASF infection is unknown. Here we combined a modeling framework with the assembled contemporary records of ASF cases and multiple covariates to predict the risk distribution of ASF at a global scale. Local spatial variations in ASF risk derived from domestic pigs is influenced strongly by livestock factors, while the risk of having ASF in wild boars is mainly associated with natural habitat covariates. The risk maps show that ASF is to be ubiquitous in many areas, with a higher risk in areas in the northern hemisphere. Nearly half of the world's domestic pigs (1.388 billion) are in the high-risk zones. Our results provide a better understanding of the potential distribution beyond the current geographical scope of the disease.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267128PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022809PMC
April 2022

Simultaneous Prediction of Wheat Yield and Grain Protein Content Using Multitask Deep Learning from Time-Series Proximal Sensing.

Plant Phenomics 2022 29;2022:9757948. Epub 2022 Mar 29.

Plant Phenomics Research Centre, Academy for Advanced Interdisciplinary Studies, Regional Technique Innovation Center for Wheat Production, Ministry of Agriculture, Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Collaborative Innovation Centre for Modern Crop Production Co-Sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing 210095, China.

Wheat yield and grain protein content (GPC) are two main optimization targets for breeding and cultivation. Remote sensing provides nondestructive and early predictions of yield and GPC, respectively. However, whether it is possible to simultaneously predict yield and GPC in one model and the accuracy and influencing factors are still unclear. In this study, we made a systematic comparison of different deep learning models in terms of data fusion, time-series feature extraction, and multitask learning. The results showed that time-series data fusion significantly improved yield and GPC prediction accuracy with values of 0.817 and 0.809. Multitask learning achieved simultaneous prediction of yield and GPC with comparable accuracy to the single-task model. We further proposed a two-to-two model that combines data fusion (two kinds of data sources for input) and multitask learning (two outputs) and compared different feature extraction layers, including RNN (recurrent neural network), LSTM (long short-term memory), CNN (convolutional neural network), and attention module. The two-to-two model with the attention module achieved the best prediction accuracy for yield ( = 0.833) and GPC ( = 0.846). The temporal distribution of feature importance was visualized based on the attention feature values. Although the temporal patterns of structural traits and spectral traits were inconsistent, the overall importance of both structural traits and spectral traits at the postanthesis stage was more important than that at the preanthesis stage. This study provides new insights into the simultaneous prediction of yield and GPC using deep learning from time-series proximal sensing, which may contribute to the accurate and efficient predictions of agricultural production.
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http://dx.doi.org/10.34133/2022/9757948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8988204PMC
March 2022

Arthroscopic modified Broström procedure achieves faster return to sports than open procedure for chronic ankle instability.

Knee Surg Sports Traumatol Arthrosc 2022 Apr 13. Epub 2022 Apr 13.

Department of Sports Medicine of Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, No.49 North Garden Road, Haidian, Beijing, 100191, China.

Purpose: To compare the clinical outcomes, rate of return to sports, postural control, and muscle strength between the arthroscopic and open modified Broström procedure for chronic lateral ankle instability (CLAI) patients.

Methods: From September 2018 to April 2019, 70 patients diagnosed with CLAI were prospectively included with arthroscopic modified Broström procedure (n = 36) and open modified Broström procedure (n = 34). They were evaluated at five time points (preoperation and 3 months, 6 months, 1 year and 2 years postoperatively). The main results examined the rate of return to sports, American Orthopaedic Foot and Ankle Society Score (AOFAS), Foot and Ankle Ability Measure (FAAM), visual analogue scale (VAS), centre of pressure (COP) excursion velocity, time to boundary (TTB), plantar pressure, isokinetic muscle strength and complications.

Results: Compared with the open group, the arthroscopic group demonstrated a significantly shorter period of return to the preinjury sport (13.2 ± 2.4 weeks vs. 18.7 ± 3.1 weeks, P = 0.023) and a higher early sport ratio (80.6 vs. 61.8%, P = 0.011) combined with better FAAM sports and AOFAS at 3 months and 6 months postoperatively and VAS at 3 months postoperatively. In addition, better anterior-posterior postural control stability, less time to peak force under lateral hindfoot and better dorsiflexion strength were shown in the arthroscopic group at 6 months postoperatively. No significant difference was found in clinical scores, posture control or muscle strength at the 1- or 2-year follow-up between the two groups.

Conclusions: Shorter period and higher rates of return to sport activities and better clinical scores, posture control and muscle strength were achieved in the arthroscopic group at 6 months postoperatively, and no clinical differences were found between arthroscopic and open modified Broström procedure 1 year or 2 years postoperatively. Arthroscopic modified Broström procedure is a reliable procedure for CLAI injuries with the demand for fast exercise recovery.

Clinical Registration: ChiCTR1900023999.

Level Of Evidence: II.
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http://dx.doi.org/10.1007/s00167-022-06961-0DOI Listing
April 2022

Relationship of Starch Pasting Properties and Dough Rheology, and the Role of Starch in Determining Quality of Short Biscuit.

Front Plant Sci 2022 28;13:829229. Epub 2022 Mar 28.

College of Agriculture, Nanjing Agricultural University, Nanjing, China.

Starch plays an important role in food industry. In this study, three wheat cultivars with different protein contents were used to investigate the different ratios of starch addition on starch pasting properties, starch thermal performance, dough rheology, biscuit quality, and their relationships. Results showed that with the increase in starch content, gluten, protein and glutenin macropolymer (GMP), lactic acid solvent retention capacity (SRC), sucrose SRC, and onset temperature (To) decreased, while most pasting parameters and gelatinization enthalpy (ΔH) increased. Viscosity parameters were significantly negatively correlated with dough stability time, farinograph quality number (FQN), and sucrose SRC. Biscuit quality was improved by starch addition, indicated by lower thickness and hardness, higher diameter, spread ratio, and sensory score. Viscosity parameters were positively correlated to diameter, spread ratio, and sensory score of biscuit, while negatively correlated to hardness and thickness of biscuit. Image analysis showed that the crumbs of biscuit were improved as shown by bigger pores in the bottom side. The results provide useful information for the clarification of the role of starch in determining biscuit quality and the inter-relationships of flour, dough, and biscuit.
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http://dx.doi.org/10.3389/fpls.2022.829229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8996254PMC
March 2022

Arabidopsis HOPS subunit VPS41 carries out plant-specific roles in vacuolar transport and vegetative growth.

Plant Physiol 2022 06;189(3):1416-1434

Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, School of Life Sciences, Institute of Plant and Food Science, Southern University of Science and Technology, Shenzhen 518055, China.

The homotypic fusion and protein sorting (HOPS) complex is a conserved, multi-subunit tethering complex in eukaryotic cells. In yeast and mammalian cells, the HOPS subunit vacuolar protein sorting-associated protein 41 (VPS41) is recruited to late endosomes after Ras-related protein 7 (Rab7) activation and is essential for vacuole fusion. However, whether VPS41 plays conserved roles in plants is not clear. Here, we demonstrate that in the model plant Arabidopsis (Arabidopsis thaliana), VPS41 localizes to distinct condensates in root cells in addition to its reported localization at the tonoplast. The formation of condensates does not rely on the known upstream regulators but depends on VPS41 self-interaction and is essential for vegetative growth regulation. Genetic evidence indicates that VPS41 is required for both homotypic vacuole fusion and cargo sorting from the adaptor protein complex 3, Rab5, and Golgi-independent pathways but is dispensable for the Rab7 cargo inositol transporter 1. We also show that VPS41 has HOPS-independent functions in vacuolar transport. Taken together, our findings indicate that Arabidopsis VPS41 is a unique subunit of the HOPS complex that carries out plant-specific roles in both vacuolar transport and developmental regulation.
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http://dx.doi.org/10.1093/plphys/kiac167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237685PMC
June 2022

Integrated Analysis of lncRNA and mRNA Expression Profiles Indicates Age-Related Changes in Meniscus.

Front Cell Dev Biol 2022 10;10:844555. Epub 2022 Mar 10.

Department of Sports Medicine, Peking University Third Hospital, Beijing, China.

Little has been known about the role of long non-coding RNA (lncRNA) involves in change of aged meniscus. Microarray analyses were performed to identify lncRNAs and mRNAs expression profiles of meniscus in young and aging adults and apple bioinformatics methods to analyse their potential roles. The differentially expressed (DE) lncRNAs and mRNAs were confirmed by qRT-PCR. A total of 1608 DE lncRNAs and 1809 DE mRNAs were identified. Functional and pathway enrichment analyses of all DE mRNAs showed that DE mRNAs were mainly involved in the TGF-beta, Wnt, Hippo, PI3K-Akt signaling pathway. The expressions of TNFRSF11B and BMP2 were significantly upregulated in aging group. LASSO logistic regression analysis of the DE lncRNAs revealed four lncRNAs (AC124312.5, HCG11, POC1B-AS1, and AP001011.1) that were associated with meniscus degradation. CNC analysis demonstrated that AP001011 inhibited the expression of TNFRSF11B and AC1243125 upregulated the expression of TNFRSF11B. CeRNA analysis suggested that POC1B-AS1 regulates the expression of BMP2 by sponging miR 130a-3p, miR136-5p, miR 18a-3p, and miR 608. Furthermore, subcellular localization and mA modification sites prediction analysis of these four lncRNAs was performed. These data lay a foundation for extensive studies on the role of lncRNAs in change of aged meniscus.
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http://dx.doi.org/10.3389/fcell.2022.844555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960627PMC
March 2022

Distal insertion rupture of lateral ankle ligament as a predictor of weakened and delayed sports recovery after acute ligament repair: mid-term outcomes of 117 cases.

BMC Musculoskelet Disord 2022 Mar 28;23(1):294. Epub 2022 Mar 28.

Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing Key Laboratory of Sports Injuries, Beijing, 100191, China.

Background: For lateral ankle ligament rupture, surgical repair has been considered for patients requiring return to high-demanding sports. However, there is a lack of systematic research regarding arthroscopic treatment followed by ligament repair for severe acute ankle sprain. The purpose of this study was to analyze the mid-term outcomes of arthroscopy followed by open anatomic lateral ankle ligament repair surgery for acute lateral ankle sprain and the impact of ligament rupture site on the outcomes.

Methods: Professional or amateur athletes with clinically- and radiologically-confirmed grade III acute lateral ankle ligament injuries undergoing ankle arthroscopy followed by open anatomic ligament repair between June 2007 and May 2017 were reviewed. Intra-articular lesions and the location of rupture were first examined under arthroscopy. Simple suture repair was performed for mid- substance ligament rupture (middle group), while suture repair with anchors were used for the ruptures near the ligament attachment site on the fibular (proximal group), talar or the calcaneal side (distal group). Outcomes were evaluated at final follow-up, including visual analog scale (VAS) pain score, American Orthopaedic Foot & Ankle Society (AOFAS) score, Tegner score, time to return to sports, resumption of pre-injury sports level, sprain recurrence and range of motion (ROM).

Results: A total of 117 patients were included for analysis, with a mean follow-up duration of 46.4 ± 16.1 months. There were 48 (41%) cases in the proximal group, 41 (35%) cases in the middle group and 28 (24%) cases in the distal group respectively. At final follow-up, all of the VAS score, AOFAS score and the Tegner score were significantly improved from the pre-operative level (p < 0.001). 12 (10%) patients complained of sprain recurrence during follow-up. 14 (12%) patients reported mild ROM restriction and 7 (6%) patients experienced transient skin numbness. The average time to return to pre-injury sports was 4.34 ± 1.11 months. The smallest proportion (86% ± 13%) of resumption of pre-injury sports level was reported from the distal group, compared with 93% ± 12% for the proximal group and 89% ± 14% for the middle group (p = 0.037). Time to return to sports was significantly longer for the distal group, with an average of 4.59 ± 1.27 months compared to 3.99 ± 1.09 months for the proximal group and 4.58 ± 0.90 months for the middle group (p = 0.009).

Conclusions: Ankle arthroscopy followed by open anatomic ligament repair is a reliable procedure for patients requiring return to high demanding sports after severe acute ankle sprains. Distal rupture near the talar or calcaneal end was associated with delayed return-to-sport and inferior performance in resuming pre-injury sports level.
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http://dx.doi.org/10.1186/s12891-022-05260-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961918PMC
March 2022
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